1. Fast blue B functionalized silica-polymer composite to evaluate 3,5-dihy-droxyhydrocinnamic acid as biomarker of gluten intake
- Author
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Carmen Ribes-Koninckx, M. Fuster-Garcia, Yolanda Moliner-Martínez, Carmen Molins-Legua, L. Hakobyan, M.C. Prieto-Blanco, María Roca Llorens, and Pilar Campíns-Falcó
- Subjects
alkylresorcinols ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,fast blue B ,Materials Chemistry ,Electrical and Electronic Engineering ,Instrumentation ,Fast blue ,Volume concentration ,chemistry.chemical_classification ,Chromatography ,Metals and Alloys ,biomarkers ,Gluten intake ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Gluten ,capillary liquid chromatography ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry ,Reagent ,Food products ,PDMS composites ,Polymer composites ,Biomarker (medicine) ,0210 nano-technology ,celiac disease - Abstract
Celiac disease is an immune-mediated systemic disorder elicited by gluten and related prolamines in genetically susceptible individuals. The current treatment is a strict and lifelong gluten-free diet. However, compliance with the gluten-free diet is not always adequate and many food products contain low concentrations of gluten. The determination of dietary transgressions is a challenge for patients, physicians and dietitians. Alkylresorcinols (AR) have been proposed as sensitive and specific biomarkers of gluten consumption. In this work silica-polymer composites doped with fast blue B reagent (FB) have been used to estimate alkylresorcinols in biological samples. The proposed colorimetric device was synthetized by immobilizing FB into polydimethylsiloxane-tetraethylortosilicate composite. The assay was based on the spontaneous release of FB to the solution con-taining AR (3,5-dihydroxyhydrocinnamic acid, DHCA) and the azocomplexe formation (520 nm). The response was evaluated with UV-vis spectroscopy and chromatography (in-tube SPME-Capillary LC-DAD) to isolate DHCA signal. The spectroscopic analysis can be used as a screening tool to differentiate positive and negative samples. Meanwhile, the chromatographic assay was necessary to isolate DHCA response in positive samples. LOD was 60 ng mL-1 by adding a SPE step. Precision provided adequate results (RSD < 15%). Preliminary studies in urine samples displayed successful results, showing DHCA can be used as biomarker of gluten intake. The here pro-posed methodology clearly simplifies the dietary transgression evaluation thanks to the development of a pre -screening tool before the chromatographic analysis.
- Published
- 2021