1. Small non-coding RNAome changes during human chondrocyte senescence as potential epigenetic targets in age-related osteoarthritis.
- Author
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Zhang, Qian-Yi, Zhou, Hao, Zhou, Xiao-Xiao, Yu, Feng-bin, Liu, Yu-Yi, Chen, Zhi-Yang, Ma, Yi-Qun, Li, Xi-Lei, and Tian, Bo
- Subjects
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AGING , *JOINTS (Anatomy) , *NON-coding RNA , *OSTEOARTHRITIS , *EPIGENETICS , *CARTILAGE regeneration - Abstract
Chondrocyte senescence is a decisive component of age-related osteoarthritis, however, the function of small noncoding RNAs (sncRNAs) in chondrocyte senescence remains underexplored. Human hip joint cartilage chondrocytes were cultivated up to passage 4 to induce senescence. RNA samples were extracted and then analyzed using small RNA sequencing and qPCR. β-galactosidase staining was used to detect the effect of sncRNA on chondrocyte aging. Results of small RNA sequencing showed that 279 miRNAs, 136 snoRNAs, 30 snRNAs, 102 piRNAs, and 5 rasiRNAs were differentially expressed in senescent chondrocytes. The differential expression of 150 sncRNAs was further validated by qPCR. Transfection of sncRNAs and β-galactosidase staining were also performed to further revealed that hsa-miR-135b-5p, SNORA80B-201, and RNU5E-1-201 have the function to restrain chondrocyte senescence, while has-piR-019102 has the function to promote chondrocyte senescence. Our data suggest that sncRNAs have therapeutic potential as novel epigenetic targets in age-related osteoarthritis. • Our research innovatively explored the sncRNA transcriptome of senescent human hip cartilage samples. • Our research involved the seldom studied piRNA in chondrocytes, revealing its role in chondrocyte senescence and OA. • Transfection of sncRNAs were performed to revealed the function of sncRNAs in chondrocyte senescence. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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