1. Expression patterns of members of the ethylene signaling–related gene families in response to dehydration stresses in cassava
- Author
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Hou Rui Shi, Xiao Guan, Xi Yan Zhang, Tian Yan Yun, Yin Dong Zhang, Jingyi Wang, Cheng Liang Li, Ren Jun Feng, Yan Jun Chen, Jiang Hui Xie, Ming Peng, Heng Zhang, Li Fang Lu, Peng He, and Meng Yun Ren
- Subjects
0106 biological sciences ,0301 basic medicine ,Leaves ,Manihot ,Time Factors ,lcsh:Medicine ,Gene Expression ,Plant Science ,Disaccharides ,01 natural sciences ,Biochemistry ,Plant Roots ,chemistry.chemical_compound ,Plant Resistance to Abiotic Stress ,Gene expression ,Plant Hormones ,lcsh:Science ,Phylogeny ,Plant Proteins ,Abiotic component ,Multidisciplinary ,Ecology ,Dehydration ,Organic Compounds ,Plant Biochemistry ,Plant Anatomy ,Plant physiology ,food and beverages ,Plants ,Chemistry ,Experimental Organism Systems ,Plant Physiology ,Physical Sciences ,Research Article ,Signal Transduction ,Proline ,Arabidopsis Thaliana ,Drought tolerance ,Carbohydrates ,Brassica ,Biology ,Research and Analysis Methods ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Ethylene ,Model Organisms ,Plant and Algal Models ,Stress, Physiological ,Plant-Environment Interactions ,Botany ,Osmotic Shock ,DNA-binding proteins ,Genetics ,Gene Regulation ,Plant Defenses ,Computer Simulation ,Gene ,Cassava ,Abiotic stress ,Plant Ecology ,Gene Expression Profiling ,lcsh:R ,fungi ,Organic Chemistry ,Ecology and Environmental Sciences ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,Trehalose ,Cell Biology ,Plant Pathology ,Ethylenes ,Hormones ,Regulatory Proteins ,Gene expression profiling ,Plant Leaves ,030104 developmental biology ,chemistry ,lcsh:Q ,Shrubs ,010606 plant biology & botany ,Transcription Factors - Abstract
Drought is the one of the most important environment stresses that restricts crop yield worldwide. Cassava (Manihot esculenta Crantz) is an important food and energy crop that has many desirable traits such as drought, heat and low nutrients tolerance. However, the mechanisms underlying drought tolerance in cassava are unclear. Ethylene signaling pathway, from the upstream receptors to the downstream transcription factors, plays important roles in environmental stress responses during plant growth and development. In this study, we used bioinformatics approaches to identify and characterize candidate Manihot esculenta ethylene receptor genes and transcription factor genes. Using computational methods, we localized these genes on cassava chromosomes, constructed phylogenetic trees and identified stress-responsive cis-elements within their 5' upstream regions. Additionally, we measured the trehalose and proline contents in cassava fresh leaves after drought, osmotic, and salt stress treatments, and then it was found that the regulation patterns of contents of proline and trehalose in response to various dehydration stresses were differential, or even the opposite, which shows that plant may take different coping strategies to deal with different stresses, when stresses come. Furthermore, expression profiles of these genes in different organs and tissues under non-stress and abiotic stress were investigated through quantitative real-time PCR (qRT-PCR) analyses in cassava. Expression profiles exhibited clear differences among different tissues under non-stress and various dehydration stress conditions. We found that the leaf and tuberous root tissues had the greatest and least responses, respectively, to drought stress through the ethylene signaling pathway in cassava. Moreover, tuber and root tissues had the greatest and least reponses to osmotic and salt stresses through ethylene signaling in cassava, respectively. These results show that these plant tissues had differential expression levels of genes involved in ethylene signaling in response to the stresses tested. Moreover, after several gene duplication events, the spatiotemporally differential expression pattern of homologous genes in response to abiotic and biotic stresses may imply their functional diversity as a mechanism for adapting to the environment. Our data provide a framework for further research on the molecular mechanisms of cassava resistance to drought stress and provide a foundation for breeding drought-resistant new cultivars.
- Published
- 2017