32 results on '"Tomasi, Nicola"'
Search Results
2. Degradation of citrate promotes copper co-precipitation within aluminium-(hydr)oxides in calcareous soils
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Terzano, Roberto, Cuccovillo, Giovanni, Pascazio, Silvia, Crecchio, Carmine, Lettino, Antonio, Fiore, Saverio, Tomasi, Nicola, Pinton, Roberto, Mimmo, Tanja, and Cesco, Stefano
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- 2017
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3. Cadmium inhibits the induction of high-affinity nitrate uptake in maize (Zea mays L.) roots
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Rizzardo, Cecilia, Tomasi, Nicola, Monte, Rossella, Varanini, Zeno, Nocito, Fabio F., Cesco, Stefano, and Pinton, Roberto
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- 2012
4. Combined effect of organic acids and flavonoids on the mobilization of major and trace elements from soil
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Terzano, Roberto, Cuccovillo, Giovanni, Gattullo, Concetta Eliana, Medici, Luca, Tomasi, Nicola, Pinton, Roberto, Mimmo, Tanja, and Cesco, Stefano
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- 2015
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5. Release of plant-borne flavonoids into the rhizosphere and their role in plant nutrition
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Cesco, Stefano, Neumann, Guenter, Tomasi, Nicola, Pinton, Roberto, and Weisskopf, Laure
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- 2010
6. Evaluation of 59 Fe-lignosulfonates complexes as Fe-sources for plants
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Rodríguez-Lucena, Patricia, Tomasi, Nicola, Pinton, Roberto, Hernández-Apaolaza, Lourdes, Lucena, Juan J., and Cesco, Stefano
- Published
- 2009
7. Micro-analytical, physiological and molecular aspects of Fe acquisition in leaves of Fe-deficient tomato plants re-supplied with natural Fe-complexes in nutrient solution
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Tomasi, Nicola, Rizzardo, Cecilia, Monte, Rossella, Gottardi, Stefano, Jelali, Nahida, Terzano, Roberto, Vekemans, Bart, De Nobili, Maria, Varanini, Zeno, Pinton, Roberto, and Cesco, Stefano
- Published
- 2009
8. Nutrient accumulation in leaves of Fe-deficient cucumber plants treated with natural Fe complexes
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Tomasi, Nicola, Mimmo, Tanja, Terzano, Roberto, Alfeld, Matthias, Janssens, Koen, Zanin, Laura, Pinton, Roberto, Varanini, Zeno, and Cesco, Stefano
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- 2014
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9. Secretion activity of white lupin's cluster roots influences bacterial abundance, function and community structure
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Weisskopf, Laure, Fromin, Nathalie, Tomasi, Nicola, Aragno, Michel, and Martinoia, Enrico
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- 2005
10. Transcriptomic Analysis Highlights Reciprocal Interactions of Urea and Nitrate for Nitrogen Acquisition by Maize Roots
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Zanin, Laura, Zamboni, Anita, Monte, Rossella, Tomasi, Nicola, Varanini, Zeno, Cesco, Stefano, and Pinton, Roberto
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- 2015
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11. Iron (Fe) speciation in xylem sap by XANES at a high brilliant synchrotron X-ray source: opportunities and limitations
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Terzano, Roberto, Mimmo, Tanja, Vekemans, Bart, Vincze, Laszlo, Falkenberg, Gerald, Tomasi, Nicola, Schnell Ramos, Magali, Pinton, Roberto, and Cesco, Stefano
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- 2013
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12. Spatially resolved (semi)quantitative determination of iron (Fe) in plants by means of synchrotron micro X-ray fluorescence
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Terzano, Roberto, Alfeld, Matthias, Janssens, Koen, Vekemans, Bart, Schoonjans, Tom, Vincze, Laszlo, Tomasi, Nicola, Pinton, Roberto, and Cesco, Stefano
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- 2013
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13. Physiological and molecular characterization of Fe acquisition by tomato plants from natural Fe complexes
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Tomasi, Nicola, De Nobili, Maria, Gottardi, Stefano, Zanin, Laura, Mimmo, Tanja, Varanini, Zeno, Römheld, Volker, Pinton, Roberto, and Cesco, Stefano
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- 2013
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14. Evaluation of 59Fe-lignosulfonates complexes as Fe-sources for plants
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Rodríguez-Lucena, Patricia, Tomasi, Nicola, Pinton, Roberto, Hernández-Apaolaza, Lourdes, Lucena, Juan J., and Cesco, Stefano
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- 2009
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15. Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium - Fabaceae Symbiotic Interaction.
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Biała-Leonhard, Wanda, Zanin, Laura, Gottardi, Stefano, de Brito Francisco, Rita, Venuti, Silvia, Valentinuzzi, Fabio, Mimmo, Tanja, Cesco, Stefano, Bassin, Barbara, Martinoia, Enrico, Pinton, Roberto, Jasiński, Michał, and Tomasi, Nicola
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RHIZOBIUM ,ROOT-tubercles ,MEDICAGO ,ISOFLAVONOIDS ,GENISTEIN ,MALNUTRITION ,LEGUMES ,PLANT exudates - Abstract
Nitrogen (N) as well as Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by, for example, forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates and are thus able to grow without supply of N or P fertilizers. The legume-rhizobial symbiosis starts with root release of isoflavonoids that act as signaling molecules perceived by compatible bacteria. Subsequently, bacteria release nod factors, which induce signaling cascades allowing the formation of functional N-fixing nodules. We report here the identification and functional characterization of a plasma membrane-localized MATE-type transporter (LaMATE2) involved in the release of genistein from white lupin roots. The LaMATE2 expression in the root is upregulated under N deficiency as well as low phosphate availability, two nutritional deficiencies that induce the release of this isoflavonoid. LaMATE2 silencing reduced genistein efflux and even more the formation of symbiotic nodules, supporting the crucial role of LaMATE2 in isoflavonoid release and nodulation. Furthermore, silencing of LaMATE2 limited the P-solubilization activity of lupin root exudates. Transport assays in yeast vesicles demonstrated that LaMATE2 acts as a proton-driven isoflavonoid transporter. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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16. Nitrate transport in cucumber leaves is an inducible process involving an increase in plasma membrane H+-ATPase activity and abundance
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Nikolic Miroslav, Cesco Stefano, Monte Rossella, Tomasi Nicola, Gottardi Stefano, Zamboni Anita, Pinton Roberto, and Varanini Zeno
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Botany ,QK1-989 - Abstract
Abstract Background The mechanisms by which nitrate is transported into the roots have been characterized both at physiological and molecular levels. It has been demonstrated that nitrate is taken up in an energy-dependent way by a four-component uptake machinery involving high- and low- affinity transport systems. In contrast very little is known about the physiology of nitrate transport towards different plant tissues and in particular at the leaf level. Results The mechanism of nitrate uptake in leaves of cucumber (Cucumis sativus L. cv. Chinese long) plants was studied and compared with that of the root. Net nitrate uptake by roots of nitrate-depleted cucumber plants proved to be substrate-inducible and biphasic showing a saturable kinetics with a clear linear non saturable component at an anion concentration higher than 2 mM. Nitrate uptake by leaf discs of cucumber plants showed some similarities with that operating in the roots (e.g. electrogenic H+ dependence via involvement of proton pump, a certain degree of induction). However, it did not exhibit typical biphasic kinetics and was characterized by a higher Km with values out of the range usually recorded in roots of several different plant species. The quantity and activity of plasma membrane (PM) H+-ATPase of the vesicles isolated from leaf tissues of nitrate-treated plants for 12 h (peak of nitrate foliar uptake rate) increased with respect to that observed in the vesicles isolated from N-deprived control plants, thus suggesting an involvement of this enzyme in the leaf nitrate uptake process similar to that described in roots. Molecular analyses suggest the involvement of a specific isoform of PM H+-ATPase (CsHA1) and NRT2 transporter (CsNRT2) in root nitrate uptake. At the leaf level, nitrate treatment modulated the expression of CsHA2, highlighting a main putative role of this isogene in the process. Conclusions Obtained results provide for the first time evidence that a saturable and substrate-inducible nitrate uptake mechanism operates in cucumber leaves. Its activity appears to be related to that of PM H+-ATPase activity and in particular to the induction of CsHA2 isoform. However the question about the molecular entity responsible for the transport of nitrate into leaf cells therefore still remains unresolved.
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- 2012
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17. Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency
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Zamboni Anita, Zanin Laura, Tomasi Nicola, Pezzotti Mario, Pinton Roberto, Varanini Zeno, and Cesco Stefano
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Plants react to iron deficiency stress adopting different kind of adaptive responses. Tomato, a Strategy I plant, improves iron uptake through acidification of rhizosphere, reduction of Fe3+ to Fe2+ and transport of Fe2+ into the cells. Large-scale transcriptional analyses of roots under iron deficiency are only available for a very limited number of plant species with particular emphasis for Arabidopsis thaliana. Regarding tomato, an interesting model species for Strategy I plants and an economically important crop, physiological responses to Fe-deficiency have been thoroughly described and molecular analyses have provided evidence for genes involved in iron uptake mechanisms and their regulation. However, no detailed transcriptome analysis has been described so far. Results A genome-wide transcriptional analysis, performed with a chip that allows to monitor the expression of more than 25,000 tomato transcripts, identified 97 differentially expressed transcripts by comparing roots of Fe-deficient and Fe-sufficient tomato plants. These transcripts are related to the physiological responses of tomato roots to the nutrient stress resulting in an improved iron uptake, including regulatory aspects, translocation, root morphological modification and adaptation in primary metabolic pathways, such as glycolysis and TCA cycle. Other genes play a role in flavonoid biosynthesis and hormonal metabolism. Conclusions The transcriptional characterization confirmed the presence of the previously described mechanisms to adapt to iron starvation in tomato, but also allowed to identify other genes potentially playing a role in this process, thus opening new research perspectives to improve the knowledge on the tomato root response to the nutrient deficiency.
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- 2012
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18. Biostimulant Action of Dissolved Humic Substances From a Conventionally and an Organically Managed Soil on Nitrate Acquisition in Maize Plants.
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Vujinović, Tihana, Zanin, Laura, Venuti, Silvia, Contin, Marco, Ceccon, Paolo, Tomasi, Nicola, Pinton, Roberto, Cesco, Stefano, and De Nobili, Maria
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DISSOLVED organic matter ,NITRITE reductase ,NITRATE reductase ,CROPS ,NITRATES - Abstract
Conversion of conventional farming (CF) to organic farming (OF) is claimed to allow a sustainable management of soil resources, but information on changes induced on dissolved organic matter (DOM) are scarce. Among DOM components, dissolved humic substances (DHS) were shown to possess stimulatory effects on plant growth. DHS were isolated from CF and OF soil leacheates collected from soil monolith columns: first in November (bare soils) and then in April and June (bare and planted soils). DHS caused an enhancement of nitrate uptake rates in maize roots and modulated several genes involved in nitrogen acquisition. The DHS from OF soil exerted a stronger biostimulant action on the nitrate uptake system, but the first assimilatory step of nitrate was mainly activated by DHS derived from CF soil. To validate the physiological response of plants to DHS exposure, real-time RT-PCR analyses were performed on those genes most involved in nitrate acquisition, such as ZmNRT2.1 , ZmNRT2.2 , ZmMHA2 (coding for two high-affinity nitrate transporters and a PM H
+ -proton pump), ZmNADH:NR , ZmNADPH:NR , and ZmNiR (coding for nitrate reductases and nitrite reductase). All tested DHS fractions induced the upregulation of nitrate reductase (NR), and in particular the OF2 DHS stimulated the expression of both tested transcripts encoding for two NR isoforms. Characteristics of DHS varied during the experiment in both OF and CF soils: a decrease of high molecular weight fractions in the OF soil, a general increase in the carboxylic groups content, as well as diverse structural modifications in OF vs. CF soils were observed. These changes were accelerated in planted soils. Similarity of chemical properties of DHS with the more easily obtainable water-soluble humic substance extracted from peat (WEHS) and the correspondence of their biostimulant actions confirm the validity of studies which employ WEHS as an easily available source of DHS to investigate biostimulant actions on agricultural crops. [ABSTRACT FROM AUTHOR]- Published
- 2020
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19. Humic Substances Contribute to Plant Iron Nutrition Acting as Chelators and Biostimulants.
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Zanin, Laura, Tomasi, Nicola, Cesco, Stefano, Varanini, Zeno, and Pinton, Roberto
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HUMUS ,PLANT nutrition ,PLANT root morphology ,PLANT physiology ,PLANT membranes ,RHIZOSPHERE - Abstract
Improvement of plant iron nutrition as a consequence of metal complexation by humic substances (HS) extracted from different sources has been widely reported. The presence of humified fractions of the organic matter in soil sediments and solutions would contribute, depending on the solubility and the molecular size of HS, to build up a reservoir of Fe available for plants which exude metal ligands and to provide Fe-HS complexes directly usable by plant Fe uptake mechanisms. It has also been shown that HS can promote the physiological mechanisms involved in Fe acquisition acting at the transcriptional and post-transcriptional level. Furthermore, the distribution and allocation of Fe within the plant could be modified when plants were supplied with water soluble Fe-HS complexes as compared with other natural or synthetic chelates. These effects are in line with previous observations showing that treatments with HS were able to induce changes in root morphology and modulate plant membrane activities related to nutrient acquisition, pathways of primary and secondary metabolism, hormonal and reactive oxygen balance. The multifaceted action of HS indicates that soluble Fe-HS complexes, either naturally present in the soil or exogenously supplied to the plants, can promote Fe acquisition in a complex way by providing a readily available iron form in the rhizosphere and by directly affecting plant physiology. Furthermore, the possibility to use Fe-HS of different sources, size and solubility may be considered as an environmental-friendly tool for Fe fertilization of crops. [ABSTRACT FROM AUTHOR]
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- 2019
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20. Transcriptional and physiological analyses of Fe deficiency response in maize reveal the presence of Strategy I components and Fe/P interactions.
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Zanin, Laura, Venuti, Silvia, Zamboni, Anita, Varanini, Zeno, Tomasi, Nicola, and Pinton, Roberto
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CORN diseases ,IRON deficiency diseases ,PHYTOSIDEROPHORES ,GENETIC transcription in plants ,PLANT molecular biology ,PLANTS - Abstract
Background: Under limited iron (Fe) availability maize, a Strategy II plant, improves Fe acquisition through the release of phytosiderophores (PS) into the rhizosphere and the subsequent uptake of Fe-PS complexes into root cells. Occurrence of Strategy-I-like components and interactions with phosphorous (P) nutrition has been hypothesized based on molecular and physiological studies in grasses. Results: In this report transcriptomic analysis (NimbleGen microarray) of Fe deficiency response revealed that maize roots modulated the expression levels of 724 genes (508 up- and 216 down-regulated, respectively). As expected, roots of Fe-deficient maize plants overexpressed genes involved in the synthesis and release of 2'-deoxymugineic acid (the main PS released by maize roots). A strong modulation of genes involved in regulatory aspects, Fe translocation, root morphological modification, primary metabolic pathways and hormonal metabolism was induced by the nutritional stress. Genes encoding transporters for Fe2+ (ZmNRAMP1) and P (ZmPHT1;7 and ZmPHO1) were also up-regulated under Fe deficiency. Fe-deficient maize plants accumulated higher amounts of P than the Fe-sufficient ones, both in roots and shoots. The supply of 1 µM 59Fe, as soluble (Fe-Citrate and Fe-PS) or sparingly soluble (Ferrihydrite) sources to deficient plants, caused a rapid down-regulation of genes coding for PS and Fe(III)-PS transport, as well as of ZmNRAMP1 and ZmPHT1;7. Levels of 32P absorption essentially followed the rates of 59Fe uptake in Fe-deficient plants during Fe resupply, suggesting that P accumulation might be regulated by Fe uptake in maize plants. Conclusions: The transcriptional response to Fe-deficiency in maize roots confirmed the modulation of known genes involved in the Strategy II and revealed the presence of Strategy I components usually described in dicots. Moreover, data here presented provide evidence of a close relationship between two essential nutrients for plants, Fe and P, and highlight a key role played by Fe and P transporters to preserve the homeostasis of these two nutrients in maize plants. [ABSTRACT FROM AUTHOR]
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- 2017
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21. Short-Term Treatment with the Urease Inhibitor N-(n-Butyl) Thiophosphoric Triamide (NBPT) Alters Urea Assimilation and Modulates Transcriptional Profiles of Genes Involved in Primary and Secondary Metabolism in Maize Seedlings.
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Zanin, Laura, Venuti, Silvia, Tomasi, Nicola, Zamboni, Anita, De Brito Francisco, Rita M., Varanini, Zeno, and Pinton, Roberto
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GENE expression ,NITROGEN fertilizers ,UREA metabolism - Abstract
To limit nitrogen (N) losses from the soil, it has been suggested to provide urea to crops in conjunction with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). However, recent studies reported that NBPT affects urea uptake and urease activity in plants. To shed light on these latter aspects, the effects of NBPT were studied analysing transcriptomic and metabolic changes occurring in urea-fed maize seedlings after a short-term exposure to the inhibitor. We provide evidence that NBPT treatment led to a wide reprogramming of plant metabolism. NBPT inhibited the activity of endogenous urease limiting the release and assimilation of ureic-ammonium, with a simultaneous accumulation of urea in plant tissues. Furthermore, NBPT determined changes in the glutamine, glutamate, and asparagine contents. Microarray data indicate that NBPT affects ureic-N assimilation and primary metabolism, such as glycolysis, TCA cycle, and electron transport chain, while activates the phenylalanine/tyrosine-derivative pathway. Moreover, the expression of genes relating to the transport and complexation of divalent metals was strongly modulated by NBPT. Data here presented suggest that when NBPT is provided in conjunction with urea an imbalance between C and N compounds might occur in plant cells. Under this condition, root cells also seem to activate a response to maintain the homeostasis of some micronutrients. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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22. Both Free Indole-3-Acetic Acid and Photosynthetic Performance are Important Players in the Response of Medicago truncatula to Urea and Ammonium Nutrition Under Axenic Conditions.
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Esteban, Raquel, Royo, Beatriz, Urarte, Estibaliz, Moran, Jose F., Zamarreño, Ángel M., Garcia-Mina, José M., Tomasi, Nicola, and Komives, Tamas Adam
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AMMONIUM ,AUXIN ,MEDICAGO truncatula - Abstract
We aimed to identify the early stress response and plant performance of Medicago truncatula growing in axenic medium with ammonium or urea as the sole source of nitrogen, with respect to nitrate-based nutrition. Biomass measurements, auxin content analyses, root system architecture (RSA) response analyses, and physiological parameters were determined. Both ammonium and ureic nutrition severely affected the RSA, resulting in changes in the main elongation rate, lateral root development, and insert position from the root base. The auxin content decreased in both urea- and ammonium-treated roots; however, only the ammonium-treated plants were affected at the shoot level. The analysis of chlorophyll a fluorescence transients showed that ammonium affected photosystem II, but urea did not impair photosynthetic activity. Superoxide dismutase isoenzymes in the plastids were moderately affected by urea and ammonium in the roots. Overall, our results showed that low N doses from different sources had no remarkable effects on M. truncatula, with the exception of the differential phenotypic root response. High doses of both ammonium and urea caused great changes in plant length, auxin contents and physiological measurements. Interesting correlations were found between the shoot auxin pool and both plant length and the "performance index" parameter, which is obtained from measurements of the kinetics of chlorophyll a fluorescence. Taken together, these data demonstrate that both the indole-3-acetic acid pool and performance index are important components of the response of M. truncatula under ammonium or urea as the sole N source. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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23. Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent.
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Zamboni, Anita, Zanin, Laura, Tomasi, Nicola, Avesani, Linda, Pinton, Roberto, Varanini, Zeno, and Cesco, Stefano
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IRON content of plants ,IRON deficiency diseases ,CHELATING agents ,TOMATOES ,RHIZOSPHERE ,PLANT roots ,PLANTS - Abstract
Background: It is well known that in the rhizosphere soluble Fe sources available for plants are mainly represented by a mixture of complexes between the micronutrient and organic ligands such as carboxylates and phytosiderophores (PS) released by roots, as well as fractions of humified organic matter. The use by roots of these three natural Fe sources (Fe-citrate, Fe-PS and Fe complexed to water-extractable humic substances, Fe-WEHS) have been already studied at physiological level but the knowledge about the transcriptomic aspects is still lacking. Results: The
59 Fe concentration recorded after 24 h in tissues of tomato Fe-deficient plants supplied with59 Fe complexed to WEHS reached values about 2 times higher than those measured in response to the supply with Fe-citrate and Fe-PS. However, after 1 h no differences among the three Fe-chelates were observed considering the59 Fe concentration and the root Fe(III) reduction activity. A large-scale transcriptional analysis of root tissue after 1 h of Fe supply showed that Fe-WEHS modulated only two transcripts leaving the transcriptome substantially identical to Fe-deficient plants. On the other hand, Fe-citrate and Fe-PS affected 728 and 408 transcripts, respectively, having 289 a similar transcriptional behaviour in response to both Fe sources. Conclusions: The root transcriptional response to the Fe supply depends on the nature of chelating agents (WEHS, citrate and PS). The supply of Fe-citrate and Fe-PS showed not only a fast back regulation of molecular mechanisms modulated by Fe deficiency but also specific responses due to the uptake of the chelating molecule. Plants fed with Fe-WEHS did not show relevant changes in the root transcriptome with respect to the Fe-deficient plants, indicating that roots did not sense the restored cellular Fe accumulation. [ABSTRACT FROM AUTHOR]- Published
- 2016
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24. The Urease Inhibitor NBPT Negatively Affects DUR3-mediated Uptake and Assimilation of Urea in Maize Roots.
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Zanin, Laura, Tomasi, Nicola, Zamboni, Anita, Varanini, Zeno, and Pinton, Roberto
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UREASE ,UREA as fertilizer ,CORN development - Abstract
Despite the widespread use of urease inhibitors in agriculture, little information is available on their effect on nitrogen (N) uptake and assimilation. Aim of this work was to study, at physiological and transcriptional level, the effects of N-(n-butyl) thiophosphoric triamide (NBPT) on urea nutrition in hydroponically grown maize plants. Presence of NBPT in the nutrient solution limited the capacity of plants to utilize urea as a N-source; this was shown by a decrease in urea uptake rate and
15 N accumulation. Noteworthy, these negative effects were evident only when plants were fed with urea, as NBPT did not alter15 N accumulation in nitrate-fed plants. NBPT also impaired the growth of Arabidopsis plants when urea was used as N-source, while having no effect on plants grown with nitrate or ammonium. This response was related, at least in part, to a direct effect of NBPT on the high affinity urea transport system. Impact of NBPT on urea uptake was further evaluated using lines of Arabidopsis overexpressing ZmDUR3 and dur3-knockout; results suggest that not only transport but also urea assimilation could be compromised by the inhibitor. This hypothesis was reinforced by an over-accumulation of urea and a decrease in ammonium concentration in NBPTtreated plants. Furthermore, transcriptional analyses showed that in maize roots NBPT treatment severely impaired the expression of genes involved in the cytosolic pathway of ureic-N assimilation and ammonium transport. NBPT also limited the expression of a gene coding for a transcription factor highly induced by urea and possibly playing a crucial role in the regulation of its acquisition. This work provides evidence that NBPT can heavily interfere with urea nutrition in maize plants, limiting influx as well as the following assimilation pathway. [ABSTRACT FROM AUTHOR]- Published
- 2015
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25. Influence of different trap solutions on the determination of root exudates in Lupinus albus L.
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Valentinuzzi, Fabio, Cesco, Stefano, Tomasi, Nicola, and Mimmo, Tanja
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PLANT exudates ,LUPINES ,PLANT roots ,EXUDATION (Botany) ,FLAVONOIDS - Abstract
White lupin is very often used as a model plant for root exudation studies due to its capability to release huge amounts of organic acids and flavonoids. The complex nature of these organic compounds makes not only their analytical determination difficult but also their extraction from soil samples. For these reasons simplified approaches, as hydroponic-based systems are widely used to study the root exudation. Therefore, the composition of a trap solution is crucial to limit artefacts causing over/underestimation of exudation rates and/or a biased molecular composition of the collected compounds. The present study was aimed at assessing the influence of different trap solutions and collection times on the quali- and quantitative root exudation pattern of white lupin ( Lupinus albus L.) grown under phosphorus (P) and iron (Fe) deficiency. Our results suggest that, in works aimed at studying root exudation processes, water is the most effective trap solution to collect the exudates like organic acids and flavonoids, especially in short time (e.g. 2 h). For longer times, low concentrations of Ca could be helpful to limit osmotic stress and possible passive leakage and/or diffusion. The use of bacteriostatic compounds as NaN and Micropur bias the results, due to interferences either with the metabolism or inhibition of the exudation processes, especially in the case of flavonoids such as quercetin. Also, the use of a pH buffer solution like 2-(N-morpholino)ethanesulfonic acid (MES) should be avoided for its undesired interferences with the release. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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26. Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review.
- Author
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Pii, Youry, Mimmo, Tanja, Tomasi, Nicola, Terzano, Roberto, Cesco, Stefano, and Crecchio, Carmine
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RHIZOSPHERE ,PLANT growth ,RHIZOBACTERIA ,SOIL microbiology ,PHYSIOLOGICAL control systems ,BIOAVAILABILITY ,MICRONUTRIENTS - Abstract
Plant growth-promoting rhizobacteria (PGPR) are soil bacteria that are able to colonize rhizosphere and to enhance plant growth by means of a wide variety of mechanisms like organic matter mineralization, biological control against soil-borne pathogens, biological nitrogen fixation, and root growth promotion. A very interesting feature of PGPR is their ability of enhancing nutrient bioavailability. Several bacterial species have been characterized as P-solubilizing microorganisms while other species have been shown to increase the solubility of micronutrients, like those that produce siderophores for Fe chelation. The enhanced amount of soluble macro- and micronutrients in the close proximity of the soil-root interface has indeed a positive effect on plant nutrition. Furthermore, several pieces of evidence highlight that the inoculation of plants with PGPR can have considerable effects on plant at both physiological and molecular levels (e.g., induction of rhizosphere acidification, up- and downregulation of genes involved in ion uptake, and translocation), suggesting the possibility that soil biota could stimulate plants being more efficient in retrieving nutrients from soil and coping with abiotic stresses. However, the molecular mechanisms underlying these phenomena, the signals involved as well as the potential applications in a sustainable agriculture approach, and the biotechnological aspects for possible rhizosphere engineering are still matters of discussion. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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27. Isolation and functional characterization of a high affinity urea transporter from roots of Zea mays.
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Zanin, Laura, Tomasi, Nicola, Wirdnam, Corina, Meier, Stefan, Komarova, Nataliya Y., Mimmo, Tanja, Cesco, Stefano, Rentsch, Doris, and Pinton, Roberto
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CORN , *UREA transporters , *PLANT roots , *NITROGEN , *MOLECULES - Abstract
Background Despite its extensive use as a nitrogen fertilizer, the role of urea as a directly accessible nitrogen source for crop plants is still poorly understood. So far, the physiological and molecular aspects of urea acquisition have been investigated only in few plant species highlighting the importance of a high-affinity transport system. With respect to maize, a worldwide-cultivated crop requiring high amounts of nitrogen fertilizer, the mechanisms involved in the transport of urea have not yet been identified. The aim of the present work was to characterize the high-affinity urea transport system in maize roots and to identify the high affinity urea transporter. Results Kinetic characterization of urea uptake (<300 μM) demonstrated the presence in maize roots of a high-affinity and saturable transport system; this system is inducible by urea itself showing higher Vmax and Km upon induction. At molecular level, the ORF sequence coding for the urea transporter, ZmDUR3, was isolated and functionally characterized using different heterologous systems: a dur3 yeast mutant strain, tobacco protoplasts and a dur3 Arabidopsis mutant. The expression of the isolated sequence, ZmDUR3-ORF, in dur3 yeast mutant demonstrated the ability of the encoded protein to mediate urea uptake into cells. The subcellular targeting of DUR3/GFP fusion proteins in tobacco protoplasts gave results comparable to the localization of the orthologous transporters of Arabidopsis and rice, suggesting a partial localization at the plasma membrane. Moreover, the overexpression of ZmDUR3 in the atdur3-3 Arabidopsis mutant showed to complement the phenotype, since different ZmDUR3-overexpressing lines showed either comparable or enhanced 15[N]-urea influx than wild-type plants. These data provide a clear evidence in planta for a role of ZmDUR3 in urea acquisition from an extra-radical solution. Conclusions This work highlights the capability of maize plants to take up urea via an inducible and highaffinity transport system. ZmDUR3 is a high-affinity urea transporter mediating the uptake of this molecule into roots. Data may provide a key to better understand the mechanisms involved in urea acquisition and contribute to deepen the knowledge on the overall nitrogenuse efficiency in crop plants. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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28. The Effect of Growth Medium Temperature on Corn Salad [Valerianella locusta (L.) Laterr] Baby Leaf Yield and Quality.
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Costa, Luisa Dalla, Tomasi, Nicola, Gottardi, Stefano, Iacuzzo, Francesco, Cortella, Giovanni, Manzocco, Lara, Pinton, Roberto, Mimmo, Tanja, and Cesco, Stefano
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SOIL temperature , *PLANT productivity , *BIOLOGICAL productivity , *AGRICULTURAL productivity , *NITRATES - Abstract
Soil temperature has a crucial impact on physiological processes and growth of plants with important consequences for plant productivity and food safety including nitrate accumulation in leaf blades of leaf vegetables. Consumer demand for high-quality, fresh-cut vegetables has increased rapidly in the last decades, and temperature modulation can help control nitrate concentration in fresh vegetables, an important trait of product safety. Corn salad plants IValerianella Iocusta (L.) Laterr., cultivar Gala] were grown at three root temperatures (15, 20, and 25 °C) in a floating system. This experimental setup allowed to directly evaluate the effect of root temperature on yield and plant quality excluding the effect on soil processes and properties. Nutrient solution was renewed weekly and kept aerated while air temperature was maintained constant at 20 °C for all treatments during the entire time of experiments. At harvest, plants were collected, the shelf life evaluated, and the nutrient uptake [NO3-, iron (Fe) from 59Fe-o,oEDDHA, and 35SO42-] and mineral content were determined. Results showed that growing conditions at 20 °C of the nutrient solution led to the best plant performance in terms of yield, nitrate content at leaf level, root biomass, leaf area, and greenness with positive effects on postharvest quality, i.e., less rapid leaf loss of greenness and leaf fresh weight (FW) loss during conservation at 4 °C. At this temperature condition of the nutrient solution, it has also been observed an enhanced functionality of mechanisms involved in the acquisition of nutrients like NO3-, Fe, and SO42-, which are known to play an important role in nitrate level in leaf tissues of crops. Plants grown at 15 °C showed minor growth, whereas the nutrient solution at 25 °C caused stress for the plants affecting negatively the quality and yield. Overall, the results obtained showed that root temperature plays a fundamental role in several plant processes that affect yield and its quality; for hydroponic system cultivations, a level of growing-medium temperature close to that of the surrounding air seems suitable. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
29. Evaluation of 59Fe-lignosulfonates complexes as Fe-sources for plants.
- Author
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Rodríguez-Lucena, Patricia, Tomasi, Nicola, Pinton, Roberto, Hernández-Apaolaza, Lourdes, Lucena, Juan J., and Cesco, Stefano
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LIGNOSULFONATES , *ETHYLENEDIAMINETETRAACETIC acid , *IRON , *MOLECULAR weights , *ACETIC acid , *CHELATES , *HYDROPONICS , *PLANT diseases , *CALCAREOUS soils , *SOILS - Abstract
Iron chlorosis is a wide-spread limiting factor of production in agriculture. To cope with this problem, synthetic chelates (like EDTA or EDDHA) of Fe are used in foliar-spray or in soil treatments; however, these products are very expensive. Therefore paper-production byproducts, like Lignosulfonates (LS), with varying content of carboxylate and sulfonate groups, were tested with respect to their ability to maintain Fe in the solution of soils and to feed plants grown in hydroponics with Fe through foliar sprays or application to the nutrient solution. Results show that LS had a low capability to solubilize 59Fe-hydroxide and that preformed 59Fe(III)-LS complexes had poor mobility through a soil column (pH 7.5) and scarce stability when interacting with soils compared to 59Fe(III)-EDDHA. However when 59Fe(III)-LS were supplied to roots in a hydroponic system, they demonstrated an even higher capability to fed Fe-deficient tomato plants than 59Fe(III)-EDDHA. Hence, data here presented indicate that the low Fe use efficiency from Fe-LS observed in soil-applications is due to interactions of these Fe-sources with soil colloids rather than to the low capability of roots to use them. Foliar application experiments of 59Fe(III)-LS or 59Fe(III)-EDTA to Fe-deficient cucumber plants show that uptake and reduction rates of Fe were similar between all these complexes; on the other hand, when 59Fe(III)-LS were sprayed on Fe-deficient tomato leaves, they showed a lower uptake rate, but a similar reduction rate, than 59Fe(III)-EDTA did. In conclusion, Fe-LS may be a valid, eco-compatible and cheap alternative to synthetic chelates in dealing with Fe chlorosis when applied foliarly or in the nutrient solution of hydroponically grown plants. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
30. Transgenerational Response to Nitrogen Deprivation in Arabidopsis thaliana.
- Author
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Massaro, Monica, De Paoli, Emanuele, Tomasi, Nicola, Morgante, Michele, Pinton, Roberto, and Zanin, Laura
- Subjects
ARABIDOPSIS thaliana ,NITROGEN ,TRANSCRIPTION factors ,ACCLIMATIZATION ,ARABIDOPSIS - Abstract
Nitrogen (N) deficiency is one of the major stresses that crops are exposed to. It is plausible to suppose that a stress condition can induce a memory in plants that might prime the following generations. Here, an experimental setup that considered four successive generations of N-sufficient and N-limited Arabidopsis was used to evaluate the existence of a transgenerational memory. The results demonstrated that the ability to take up high amounts of nitrate is induced more quickly as a result of multigenerational stress exposure. This behavior was paralleled by changes in the expression of nitrate responsive genes. RNAseq analyses revealed the enduring modulation of genes in downstream generations, despite the lack of stress stimulus in these plants. The modulation of signaling and transcription factors, such as NIGTs, NFYA and CIPK23 might indicate that there is a complex network operating to maintain the expression of N-responsive genes, such as NRT2.1, NIA1 and NIR. This behavior indicates a rapid acclimation of plants to changes in N availability. Indeed, when fourth generation plants were exposed to N limitation, they showed a rapid induction of N-deficiency responses. This suggests the possible involvement of a transgenerational memory in Arabidopsis that allows plants to adapt efficiently to the environment and this gives an edge to the next generation that presumably will grow in similar stressful conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
31. Molecular and physiological interactions of urea and nitrate uptake in plants.
- Author
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Pinton, Roberto, Tomasi, Nicola, and Zanin, Laura
- Published
- 2016
- Full Text
- View/download PDF
32. Copper accumulation in vineyard soils: Rhizosphere processes and agronomic practices to limit its toxicity.
- Author
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Brunetto, Gustavo, Bastos de Melo, George Wellington, Terzano, Roberto, Del Buono, Daniele, Astolfi, Stefania, Tomasi, Nicola, Pii, Youry, Mimmo, Tanja, and Cesco, Stefano
- Subjects
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COPPER bioaccumulation , *VINEYARDS , *SOIL testing , *FUNGICIDES , *RHIZOSPHERE , *COPPER poisoning - Abstract
Viticulture represents an important agricultural practice in many countries worldwide. Yet, the continuous use of fungicides has caused copper (Cu) accumulation in soils, which represent a major environmental and toxicological concern. Despite being an important micronutrient, Cu can be a potential toxicant at high concentrations since it may cause morphological, anatomical and physiological changes in plants, decreasing both food productivity and quality. Rhizosphere processes can, however, actively control the uptake and translocation of Cu in plants. In particular, root exudates affecting the chemical, physical and biological characteristics of the rhizosphere, might reduce the availability of Cu in the soil and hence its absorption. In addition, this review will aim at discussing the advantages and disadvantages of agronomic practices, such as liming, the use of pesticides, the application of organic matter, biochar and coal fly ashes, the inoculation with bacteria and/or mycorrhizal fungi and the intercropping, in alleviating Cu toxicity symptoms. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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