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Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a new human disease with few effective treatments.sup.1. Convalescent plasma, donated by persons who have recovered from COVID-19, is the acellular component of blood that contains antibodies, including those that specifically recognize SARS-CoV-2. These antibodies, when transfused into patients infected with SARS-CoV-2, are thought to exert an antiviral effect, suppressing virus replication before patients have mounted their own humoral immune responses.sup.2,3. Virus-specific antibodies from recovered persons are often the first available therapy for an emerging infectious disease, a stopgap treatment while new antivirals and vaccines are being developed.sup.1,2. This retrospective, propensity score-matched case-control study assessed the effectiveness of convalescent plasma therapy in 39 patients with severe or life-threatening COVID-19 at The Mount Sinai Hospital in New York City. Oxygen requirements on day 14 after transfusion worsened in 17.9% of plasma recipients versus 28.2% of propensity score-matched controls who were hospitalized with COVID-19 (adjusted odds ratio (OR), 0.86; 95% confidence interval (CI), 0.75-0.98; chi-square test P value = 0.025). Survival also improved in plasma recipients (adjusted hazard ratio (HR), 0.34; 95% CI, 0.13-0.89; chi-square test P = 0.027). Convalescent plasma is potentially effective against COVID-19, but adequately powered, randomized controlled trials are needed. Convalescent plasma for treatment of hospitalized patients with COVID-19 is associated with improved survival in a retrospective comparison with matched controls, supporting further study in randomized controlled trials., Author(s): Sean T. H. Liu [sup.1] [sup.2] , Hung-Mo Lin [sup.3] , Ian Baine [sup.4] , Ania Wajnberg [sup.5] , Jeffrey P. Gumprecht [sup.1] , Farah Rahman [sup.1] , Denise [...]

This study sought to determine whether deficient Igf1 expression in osteocytes would affect loading-induced osteogenic response. Tibias of osteocyte Igf1 conditional knockout (KO) mice (generated by cross-breeding Igf1 floxed mice with Dmp1-Cre transgenic mice) and wild-type (WT) littermates were subjected to four-point bending for 2 wk. Microcomputed tomography confirmed that the size of tibias of conditional mutants was smaller. Loading with an equivalent loading strain increased periosteal woven bone and endosteal lamellar bone formation in WT mice but not in conditional KO mice. Consistent with the lack of an osteogenic response, the loading failed to upregulate expression of early mechanoresponsive genes (Igf1, Cox-2, c-fos) or osteogenic genes (Cbfa-1, and osteocalcin) in conditional KO bones. The lack of osteogenic response was not due to reduced osteocyte density or insufficient loading strain. Deficient osteocyte Igf1 expression reduced the loading-induced upregulation of expression of canonical Wnt signaling genes (Wnt10b, Lrp5, Dkk1, sFrp2). The loading also reduced (by 40%) Sost expression in WT mice, but the loading not only did not reduce but upregulated (~1.5-fold) Sost expression in conditional KO mice. Conditional disruption of Igf1 in osteocytes also abolished the loading-induced increase in the bone β-catenin protein level. These findings suggest an impaired response in the loading-induced upregulation of the Wnt signaling in conditional KO mice. In summary, conditional disruption of Igf1 in osteocytes abolished the loading-induced activation of the Wnt signaling and the corresponding osteogenic response. In conclusion, osteocyte-derived IGF-I plays a key determining role in bone mechanosensitivity. [ABSTRACT FROM AUTHOR]

This study was conducted to investigate the salt tolerance of five cotton genotypes [three Gossypium hirsutum L. (DN 1, DP 491, and FM 989) and two G. barbadense L. (Cobalt and Pima S-7)] under NaCl or Na2SO4 salinity conditions at similar osmotic potentials (100 mM NaCl vs. 70 mM Na2SO4 and 150 mM NaCl vs. 111 Na2SO4). To investigate whether the addition of calcium sulfate could alleviate the deleterious salinity effect, two more treatments were prepared by adding 10 mM CaSO4 to 150 mM NaCl and 111 mM Na2SO4 solutions. All genotypes had significant growth reduction in all salt treatments as compared to their respective controls. Whereas Upland and Pima cotton did not differ in response to salt, DP 491 had lower growth reduction as compared to other genotypes and was therefore more salt tolerant. Salt type did not affect the growth of FM 989 and Pima S-7; however, dry weight (DW) of all organs were reduced to a greater extent by NaCl than by Na2SO4 in most organs in Pima Cobalt, shoot and total DW in DP 491, and root DW in DN 1. The addition of CaSO4 alleviated some detrimental effects in DN 1 caused by NaCl and in Pima Cobalt caused by Na2SO4. The five genotypes also responded to salt treatments differently in that DN 1 and DP 491 had higher Na+ and Cl- concentrations and higher leaf osmotic potentials than the other three genotypes except for higher Cl- concentrations in Cobalt. These results indicated that diversity of salt-tolerant mechanisms existed among the five cotton genotypes. [ABSTRACT FROM AUTHOR]

Two greenhouse experiments were conducted to quantify growth responses of Jatropha curcas to a range of salt and drought stresses. Typical symptoms of salinity stress such as leaf edge yellowing were observed in all elevated salinity treatments and the degree of the foliar salt damage increased with the salinity of irrigation water. Total dry weight (DW) of Jatropha plants was reduced by 30%, 30%, and 50%, respectively, when irrigated with saline solutions at electrical conductivity of 3.0, 6.0, and 9.0 dS m-1 compared to that in the control. Leaf Na+ concentration was much higher than that observed in most glycophytes. Leaf Cl- concentrations were also high. In the drought stress experiment, plants were irrigated daily with nutrient solution at 100%, 70%, 50%, or 30% daily water use (DWU). Deficit irrigation reduced plant growth and leaf development. The DW of leaves, roots, and total were reduced in the 70%, 50%, and 30% DWU compared to the 100% DWU control treatment. In summary, salinity stress and deficit irrigation significantly reduced the growth and leaf development of greenhouse-grown Jatropha plants. [ABSTRACT FROM AUTHOR]

The growth and physiological responses of four maize inbred lines (CUBA1, B73, B5C2, and BR1) and four sorghum hybrids (SS304, NK7829, Sordan 79, and KS585) to salinity were determined. Fifteen days after sowing, seedlings were irrigated with nutrient solution (control) at electrical conductivity (EC) of 1.5 dSm-1 or saline solution at EC of 8.0 dSm-1 (salt treatment) for 40 days. Dry weight of shoots in maize was reduced by 58%, 65%, 62%, and 69% in CUBA1, B73, B5C2, and BR1, respectively, while that of sorghum was reduced by 51%, 56%, 56%, and 76% in SS304, NK7829, Sordan79, and KS585, respectively, in the salt treatment compared to their respective control. Salinity stress reduced all or some of the gas exchange parameters, leaf transpiration (E), stomatal conductance (gs), and net photosynthetic rate (Pn) in the late part of the experiment for both crops. Salinity treatment greatly increased Na+ uptake in allmaize genotypes but did not affect the Na+ uptake in sorghum, regardless of genotype. Inmaize, CUBA1 was slightly more resistant to salt stress, while BR1 was more sensitive to salt stress. In sorghum, Sordan79 was the most tolerant genotype, and KS585 was the least tolerant genotype. [ABSTRACT FROM AUTHOR]

High soil salinity often results in poor stand establishment, reduced plant growth, and reduced yield of many horticultural crops such as peppers (Capsicum annuum). We investigated the effects of soil salinity and soil type on seedling emergence and growth of four commercial peppers ('NuMex Joe E. Parker', 'NuMex Nematador', 'NuMex Primavera', and 'Jupiter') in greenhouse experiments. Seeds were sown in either a loamy sand or a silt loam soil in pots and irrigated with saline solutions at electrical conductivity of 0.9 (tap water), 3.0, or 6.0 dS.m&#sup1; (Expt. 1) or at 0.0 [reverse osmosis (RO) water], 0.9, or 1.5 dS.m&#sup1; (Expt. 2). No seedling emergence was observed in treatments irrigated with 3.0 or 6.0 dS.m&#sup1; solutions. The salinity at the top soil layer increased linearly with time when subirrigated with tap and saline solutions in both soil types, whereas no substantial increase in soil salinity was found when subirrigated with RO water or overhead irrigation with tap water. Salt accumulation at the top soil layer was greater in loamy sand than in silt loam. Seedling emergence percent subirrigated with RO water ranged from 70% to 80% in loamy sand and 45% to 70% in silt loam, depending on pepper cultivars. When subirrigated with tap water and saline solutions, the emergence percent ranged from 0% to 60%, depending on pepper and soil types. In Expt. 3, seedlings were germinated in commercial potting mix and grown in 1.8-L pots containing commercial potting mix. Saline solution treatments of 1.4 (control, nutrient solution), 2. 1, 2.9, 3.5, or 4.2 dSm&#sup1; were initiated when seedlings had 11 to 13 leaves. Five weeks after initiating saline water irrigation, the reduction in shoot dry weight was greater in 'Jupiter' and 'NuMex Primavera' as compared with 'NuMex Joe E. Parker' and 'NuMex Nematador', but the differences were small. [ABSTRACT FROM AUTHOR]

Bedding plants are extensively used in urban landscapes. As high-quality water supply becomes limited in many parts of the world, the use of recycled water with high salt levels for landscape irrigation is being encouraged. Therefore, information on salt tolerance of bedding plants is of increasing importance. Two experiments were conducted, one in a 25% light exclusion shadehouse in summer (Expt. 1) and the other in a greenhouse in winter (Expt. 2). Plants were irrigated with saline solution at electrical conductivities of 0.8, 2.8, 4.0, 5.1, or 7.4 dS·m-1 created by adding NaCI, MgSO4, and CaCI2 to tap water to simulate the composition of local reclaimed water. In Expt. 1, shoot dry weight (DW) at the end of the experiments was reduced in all species at 7.4 dS·m-1 compared with the control (0.8 dS·m-1). The magnitude of reduction varied with species and cultivars. The salinity thresholds of irrigation water in which growth reduction occurred were 4.0 dS·m-1 for angelonia (Angelonia angustifolia) cultivars and ornamental pepper (Capsicum annuum) 'Calico' and 4.0 to 5.1 dS·m-1 for helenium (Helenium amarum), licorice plant (Helichrysum petiolatum), and plumbago (Plumbago auriculata). Shoot DW and growth index of ornamental pepper 'Black Pearl' and vinea (Catharanthus roseus) 'Rose' decreased linearly as salinity increased. All plants survived in Expt. 1 regardless of treatment, except for ornamental pepper 'Purple Flash'. No visual injuries were observed in Expt. 1 regardless of treatment. Leaf sodium (Na) and chlorine (CI) concentrations varied with species and treatments. Ornamental pepper 'Black Pearl' had the highest leaf CI concentrations at higher salinities compared with other species and cultivars. Leaf Na concentrations in licorice plant and plumbago were in the range of 10 to 30 g·kg-1 DW, higher than those in other species. In Expt. 2, shout DW was reduced by salinity, treatments in ornamental pepper 'Black Pearl', plumbago, and angelonia but not in other species. The three ornamental peppers, 'Black Pearl', 'Calico', and 'Purple Flash', exhibited slight follar injuries on some plants in Expt. 2 as a result of high salinity in the root zone in the highest salinity treatment. Ornamental pepper 'Black Pearl' was most sensitive to salinity, stress. In general, the bedding plants tested in this study are moderately tolerant to salt stress and may be irrigated with saline water up to 4.0 dS·m-1 with little reduction in aesthetical appearance. [ABSTRACT FROM AUTHOR]

Use of reclaimed water to irrigate urban landscapes will likely increase because fresh water supply is diminishing and the population continues to grow in the semiarid southwestern United States. Salt tolerance of two native landscape woody ornamentals, Texas mountain laurel (Sophora secundiflora) and Mexican redbud (Cercis canadensis var. mexicana), was investigated in a greenhouse experiment. Seedlings of the two species were grown in two substrates mixed with composted mulch and a commercial potting mix at two ratios and irrigated with saline solutions at three salinity levels: 1.6 (control, nutrient solution), 3.0, or 6.0 dS*m-1 electrical conductivity (EC). There was no interaction between substrate and EC of irrigation water. Foliar salt damages such as leaf drop, leaf curl, and edge burn were observed in Mexican redbud when the plants were irrigated with solutions at EC of 3.0 and 6.0 dS*m-1. No symptoms were observed on Texas mountain laurel plants, although plants irrigated at EC of 3.0 and 6.0 dS*m-1 were smaller compared with controls. Shoot growth and elongation of both species were reduced by the elevated salinity of irrigation water, and the reduction in Mexican redbud was greater than Texas mountain laurel. Leaf photosynthesis rate and leaf stomatal conductance were also reduced in Texas mountain laurel by the elevated salinity of irrigation water. Tissue Na+ and Cl- concentrations were higher in Texas mountain laurel irrigated with water of elevated salinity. [ABSTRACT FROM AUTHOR]

Use of recycled water to irrigate urban landscapes may be inevitable, because the freshwater supply has been diminishing and the population continues to grow in the arid and semiarid southwestern United States. However, little information exists on the performance of landscape plants irrigated with nonpotable water. Two greenhouse studies were conducted during the summer and the fall to characterize the relative salt tolerance of five herbaceous perennials by irrigating the plants with a saline solution at an electrical conductivity (EC) of 0.8 dS·m-1 (tap water), 2.0 dS·m-1, or 4.0 dS·m-1. In the summer study, after 10 weeks of treatment, Achillea millefolium L., Gaillardia aristata Foug., and Salvia coccinea Juss ex J. had an aesthetically acceptable appearance for landscape performance (visual quality scores of 4 points or more), whereas Agastache cana (Hook.) Woot. & Standl. and Echinacea purpurea (L.) Moench had relatively low tolerance to salinity. Dry weight of shoots ofA. miilefolium, A. cana, and (7. arstata was lower at elevated salinity levels. In the fall study, A. millefolium, E. purpurea, {7,. arstata, and S. coccinea had acceptable growth and visual quality at elevated salinity levels, whereas A. cana had lower quality and reduced growth. Dry weight of shoots was lower in G. arstata and A. millefolium at an EC of 2.0 dS·m-1or 4.0 dS·m-1. Leaf osmotic potential of all species in the summer experiment was significantly lower at higher salinity compared with the control. In the fall experiment, leaf osmotic potential in A. millefolium, E. purpurea, and G. aristata at 4 dS·m-1 was lower compared with lower salinity treatment and the control. Leaf osmotic potential in the fall was higher than that of the same species at the same salinity level in the summer experiment, indicating that plants in the fall were less stressed than in the summer. Combined the results from both experiments, the authors concluded that A. millefolium, G. arstata, and S. coccinea had a relatively high salt tolerance (as much as 4 dS·m-1 of irrigation water under greenhouse conditions) among the tested species, whereas A. cana and E. purpurea were not tolerant to salt and should not be irrigated with low-quality water. [ABSTRACT FROM AUTHOR]

The effect of drought on the growth and gas exchange of six bedding plant species--agastache [Agastache urticifolia (Benth.) O. Kuntze 'Honeybee Blue'], dusty miller (Cineraria maritima L. 'Silverdusty'), petunia (Petunia xhybrida 'Wave Purple'), plumbago (Plumbago auriculata Lam. 'Escapade'), ornamental pepper (Capsicum annuum L. 'Black Pearl'), and vinca [Catharanthus roseus (L.) G. Don 'Titan'l--was quantified under greenhouse conditions. Seeds were sown in January and seedlings were grown in the greenhouse until 18 Apr., when two irrigation treatments--drought (D, ≈18% volumetric moisture content at reirrigation) and control (C, ≈25% volumetric moisture content at reirrigation)--were initiated. Leaf net photosynthetic rate (P.), stomatal conductance (gs), and transpiration (E) were determined in response to a range of substrate moisture content (from ≈5% to 30% by volume) and temperature (from 20 °C to 40 °C). Dry weight of agastache, ornamental pepper, and vinca was unaffected by drought, whereas that of other species was reduced. Leaf area of plumbago and height of plumbago and vinca were reduced by drought. As substrate moisture content decreased from 25% to 10%, Pn, E, and gs decreased linearly in all species except petunia and plumbago. Leaf net photosynthetic rate of all species declined as leaf temperature increased from 20 °C to 40 °C. In contrast, E of all species, except petunia, increased as temperature increased. Transpiration rate of petunia increased as temperature increased from 20 °C to 30 °C, and then decreased between 30 °C and 40 °C. Although petunia had the highest Pn among the tested species, its Pn and gs declined more rapidly compared with the other species as temperature increased from 20 °C to 40 °C or as substrate moisture content decreased, indicating that petunia was most sensitive to high temperature and drought. [ABSTRACT FROM AUTHOR]

Use of recycled water to irrigate urban landscapes and nursery plants may be inevitable as fresh water supplies diminish and populations continue to grow in the arid and semiarid southwestern United States. Lupinus havardii Wars. (Big Bend bluebonnet) has potential as a cut flower and Lupinus texensis Hook. (Texas bluebonnet) as a bedding plant, but little information is available on salt tolerance of these species. A greenhouse study was conducted to characterize the growth in response to various salinity levels. Plants were grown in 10-L containers and drip-irrigated with synthesized saline solutions at electrical conductivity levels of 1.6, 3.7, 5.7, 7.6, or 9.4 dS⋅m-1. Although shoot growth of L. texensis was reduced as salinity levels increased, it was visually acceptable (without any visual injury) when irrigated with salinity levels of less than 7.6 dS⋅m-1. All plants survived at 7.6 dS⋅m-1, whereas only 15% did at 9.4 dS⋅m-1. In contrast, L. havardii had leaf injury at 5.7 dS⋅m-1. No plants survived at 9.4 dS⋅m-1, and only 7% plants survived at 7.6 dS⋅m-1. In addition, growth of L. havardii was significantly reduced and plants were shorter at elevated salinity levels. Cut raceme yield of L. havardii decreased at salinity levels greater than 3.7 dS⋅m-1. However, no difference in cut raceme yield was observed between the control and 3.7 dS⋅m-1, although shoot growth was reduced. Overall, L. texensis was more salt-tolerant than L. havardii. [ABSTRACT FROM AUTHOR]

As high-quality water supply becomes limited in many regions of the world, alternative water sources are being used for irrigating urban landscapes. Therefore, salt-tolerant landscape plants are needed. Two greenhouse experiments were conducted to screen the salt tolerance of Zinnia marylandica ('Zahara Coral Rose', 'Zahara Fire', 'Zahara Scarlet', 'Zahara Starlight', 'Zahara White', and 'Zahara Yellow') and Z. maritima 'Solcito'. In Expt. 1, plants were subirrigated with nutrient or saline solutions at electrical conductivity (EC) at 1.4 (base nutrient solution, control), 3.0, 4.2, 6.0, or 8.2 dS·m-1 for 4 weeks, whereas in Expt. 2, plants were surface-irrigated with the same nutrient or saline solutions for 4 weeks. In Expt. 1, all plants, regardless of cultivar, died by the end of the treatment at EC 6.0 and EC 8.2 as a result of high salinity in the root zone. Plants became shorter and more compact as EC of irrigation water increased. Shoot dry weight of all cultivars in EC 4.2 was reduced by 50% to 56% compared with that of the control. Shoot Na+ and Cl- accumulated excessively as salinity increased in the irrigation water, whereas Ca2+, Mg2+, and K+ did not change substantially. In Expt. 2, mortality varied with cultivar and treatment. Similar to Expt. 1, growth reduction resulting from elevated salinity across cultivars was found. Therefore, it is concluded that zinnia cultivars used in this study are sensitive to salinity and should not be planted in areas with high soil salinity or when alternative waters with high salinity may be used for irrigation. [ABSTRACT FROM AUTHOR]

Ornamental chile peppers are popular bedding plants. As high-quality water supply becomes limited in many parts of the world, alternative waters such as municipal reclaimed water is encouraged to be used for landscape irrigation. The purpose of this study was to assess the relative salt tolerance of 10 cultivars of ornamental chile peppers by irrigating the mature plants with saline solutions and germinating seeds in saline substrate in a greenhouse. In the mature plant salt tolerance experiment, plants were irrigated with nutrient solution (no addition of salts, control) or saline solution at electrical conductance (EC) of 4.1 dS⋅m-1 or 8.1 dS⋅m-1 for 8 weeks. Plants in the EC of 4.1 dS⋅m-1 treatment did not have any foliar salt damage regardless of cultivar. At EC of 8.1 dS⋅m-1, 'NuMex Memorial Day' had the most severe foliar salt damage, whereas 'NuMex April Fool's Day', 'NuMex Cinco de Mayo', 'NuMex Thanksgiving', and 'NuMex Twilight' had little or no foliar damage. Shoot dry weight (DW) reduction at EC of 8.1 dS⋅m-1 compared with control was smallest in 'NuMex Thanksgiving' (15%), whereas 'NuMex Memorial Day' had the greatest reduction of 74% followed by 'NuMex Christmas' of 61%. The highest shoot DW reduction in 'NuMex Memorial Day' coincided with lowest visual score, indicating that this cultivar was the least tolerant to salinity. The leaf Na+ and Cl- concentrations increased dramatically with increasing EC of the irrigation water in all cultivars. The highest Na+ concentration of 10.9 mg⋅g-1 DW at EC of 8.1 dS⋅m-1 was observed in 'NuMex Christmas'. The highest Cl- concentration at EC of 8.1 dS⋅m-1 was found in 'NuMex Memorial Day' with 64.8 mg⋅g-1 DW, which was four times higher than the control. In the seedling emergence experiment, seeds of the 10 cultivars were germinated in substrate either moistened with reverse osmosis water (EC ≈0) or saline solution at EC of 17.1 dS⋅m-1 'NuMex Christmas' and 'NuMex Memorial Day' had the lowest relative seedling emergence index, indicating that these two cultivars were the least tolerant to salinity during the seedling emergence stage. 'NuMex Thanksgiving' and 'NuMex Cinco de Mayo' had the highest relative seedling emergence index. Combining the results from both experiments, we concluded that 'NuMex Cinco de Mayo' and 'NuMex Thanksgiving' were the most tolerant cultivars, whereas 'NuMex Christmas' and 'NuMex Memorial Day' were the least tolerant ones. [ABSTRACT FROM AUTHOR]