Integralno pšenično brašno dobijeno mlevenjem celog zrna predstavlja bogat izvor hranljivih materija, prehrambenih vlakana, kompleksnih ugljenih hidrata, proteina, vitamina B grupe, minerala, kao i antioksidanata koji igraju važnu ulogu u prevenciji karcinoma i kardiovaskularnih bolesti. Normalni pšenični skrob se sastoji u proseku od 22–35% amiloze, dok voštani skrob sadrži oko 100% amilopektina. Različiti odnos osnovnih komponenata skroba, amiloze i amilopektina, kao i struktura skrobnih granula mogu uticati na funkcionalna svojstva, fizičko-hemijske karakteristike brašna, kvalitet i ukus krajnjeg proizvoda. U ovom istraživanju ispitivano jeintegralno brašno šestgenotipova pšenice, od toga tri standardnog sadržaja amiloze (22%,24% i 28%), jedan visokoamilozni (36,5% amiloze) i dva voskovca(100% amilopektin).Paramtri ispitivanih uzoraka poređeni su sa komercijalnim rafinisanim brašnom (21,5% amiloze).Nakon određivanja hemijskog sastava, analiziran je kapacitetbrašna na upijanjerazličitih rastvarača (SRC -solvent retention capacity), i izračunat jeindeks učinka glutena (GPI - gluten performance index).Kapacitet apsorpcije vode kreatao se od 72,61 do 84,16%, mlečne kiseline od 77,01 do 91,81%, natrijum karbonata od 79,92 do 97,83%, saharoze od 93,78 do 116,20%, dok je GPI varirao od 0,43 do 0,45. Sadržaj vlakana bio je u negativnoj korelaciji sa ispitivanim parametrima koji utiču na funkcionalna svojstva brašna. Na osnovu vrednosti upijanja različitih rastvarača, kao i indeksa učinka glutena, integralna brašna ispitivanih genotipova pšenice mogu naći primenu prvenstveno u proizvodnji hleba, kao i u konditorskoj industriji. Ova metoda može biti korisna u procesu selekcije genotipova pšenice kada su u fokusu funkcionalna svojstava brašna neophodna za poboljšanje kvaliteta pojedinih pekarskih proizvoda., Wheat flour obtained by grinding the whole grain is a rich source of nutrients, dietary fiber, complex carbohydrates, proteins, B-group vitamins, minerals, and antioxidants that play an important role in the prevention of cancer and cardiovascular diseases. Normal wheat starch consists of an average of 22–35% amylose, while waxy starch contains about 100% amylopectin. The different ratio of the basic components of starch, amylose and amylopectin, as well as the starch granules’structure can affect the functional, physical and chemical properties of flour, quality and taste of the final product. In this research, whole-grain flour of six genotypes of wheat was examined, of which three had standard amylose content (22%, 24% and 28%), one high-amylose (36.5%) and two waxy (100% amylopectin). The parameters of the tested samples were compared with commercial refined flour (21.5% amylose). After determining the chemical composition, the solvent retention capacities of the flour (SRC) were analyzed, and their gluten performance index (GPI) was calculated. The water SRC ranged from 72.61 to 84.16%, lactic acid from 77.01 to 91.81%, sodium carbonate from 79.92 to 97.83%, sucrose from 93.78 to 116.20%, while the GPI varied from 0.43 to 0.45. The fiber content was negatively correlated with the tested parameters that affect the flour’s functional properties. Based on the SRC values of different solvents, and the GPI, whole-grain flour of the tested wheat genotypes can be used primarily in bread production, and in the confectionery industry. This method can be useful in the wheat breeding process with focus on the flour’sfunctional properties necessary for improving the quality of certain bakery products.