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18 results on '"Zou, Xiangang"'

1. LYVE-1+ macrophages form a collaborative CCR5-dependent perivascular niche that influences chemotherapy responses in murine breast cancer

Author

Anstee, Joanne E., Feehan, Karen T., Opzoomer, James W., Dean, Isaac, Muller, Henrike P., Bahri, Meriem, Cheung, Tik Shing, Liakath-Ali, Kifayathullah, Liu, Ziyan, Choy, Desmond, Caron, Jonathan, Sosnowska, Dominika, Beatson, Richard, Muliaditan, Tamara, An, Zhengwen, Gillett, Cheryl E., Lan, Guocheng, Zou, Xiangang, Watt, Fiona M., Ng, Tony, Burchell, Joy M., Kordasti, Shahram, Withers, David R., Lawrence, Toby, and Arnold, James N.

Published
2023
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2. LYVE-1+ macrophages form a collaborative CCR5-dependent perivascular niche that influences chemotherapy responses in murine breast cancer.

Author

Anstee, Joanne E, Feehan, Karen T, Opzoomer, James W, Dean, Isaac, Muller, Henrike P, Bahri, Meriem, Cheung, Tik Shing, Liakath-Ali, Kifayathullah, Liu, Ziyan, Choy, Desmond, Caron, Jonathan, Sosnowska, Dominika, Beatson, Richard, Muliaditan, Tamara, An, Zhengwen, Gillett, Cheryl E, Lan, Guocheng, Zou, Xiangang, Watt, Fiona M, Ng, Tony, Burchell, Joy M, Kordasti, Shahram, Withers, David R, Lawrence, Toby, Arnold, James N, Anstee, Joanne E, Feehan, Karen T, Opzoomer, James W, Dean, Isaac, Muller, Henrike P, Bahri, Meriem, Cheung, Tik Shing, Liakath-Ali, Kifayathullah, Liu, Ziyan, Choy, Desmond, Caron, Jonathan, Sosnowska, Dominika, Beatson, Richard, Muliaditan, Tamara, An, Zhengwen, Gillett, Cheryl E, Lan, Guocheng, Zou, Xiangang, Watt, Fiona M, Ng, Tony, Burchell, Joy M, Kordasti, Shahram, Withers, David R, Lawrence, Toby, and Arnold, James N

Abstract

Tumor-associated macrophages (TAMs) are a heterogeneous population of cells that facilitate cancer progression. However, our knowledge of the niches of individual TAM subsets and their development and function remain incomplete. Here, we describe a population of lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1)-expressing TAMs, which form coordinated multi-cellular "nest" structures that are heterogeneously distributed proximal to vasculature in tumors of a spontaneous murine model of breast cancer. We demonstrate that LYVE-1 + TAMs develop in response to IL-6, which induces their expression of the immune-suppressive enzyme heme oxygenase-1 and promotes a CCR5-dependent signaling axis, which guides their nest formation. Blocking the development of LYVE-1 + TAMs or their nest structures, using gene-targeted mice, results in an increase in CD8 + T cell recruitment to the tumor and enhanced response to chemotherapy. This study highlights an unappreciated collaboration of a TAM subset to form a coordinated niche linked to immune exclusion and resistance to anti-cancer therapy.

Published
2023

3. LYVE-1+ macrophages form a collaborative CCR5-dependent perivascular niche that influences chemotherapy responses in murine breast cancer.

Author

Afd Pharmacology, Pharmacology, Anstee, Joanne E, Feehan, Karen T, Opzoomer, James W, Dean, Isaac, Muller, Henrike P, Bahri, Meriem, Cheung, Tik Shing, Liakath-Ali, Kifayathullah, Liu, Ziyan, Choy, Desmond, Caron, Jonathan, Sosnowska, Dominika, Beatson, Richard, Muliaditan, Tamara, An, Zhengwen, Gillett, Cheryl E, Lan, Guocheng, Zou, Xiangang, Watt, Fiona M, Ng, Tony, Burchell, Joy M, Kordasti, Shahram, Withers, David R, Lawrence, Toby, Arnold, James N, Afd Pharmacology, Pharmacology, Anstee, Joanne E, Feehan, Karen T, Opzoomer, James W, Dean, Isaac, Muller, Henrike P, Bahri, Meriem, Cheung, Tik Shing, Liakath-Ali, Kifayathullah, Liu, Ziyan, Choy, Desmond, Caron, Jonathan, Sosnowska, Dominika, Beatson, Richard, Muliaditan, Tamara, An, Zhengwen, Gillett, Cheryl E, Lan, Guocheng, Zou, Xiangang, Watt, Fiona M, Ng, Tony, Burchell, Joy M, Kordasti, Shahram, Withers, David R, Lawrence, Toby, and Arnold, James N

Published
2023

4. Establishment of mouse expanded potential stem cells

Author

Yang, Jian, Ryan, David J., Wang, Wei, Tsang, Jason Cheuk-Ho, Lan, Guocheng, Masaki, Hideki, Gao, Xuefei, Antunes, Liliana, Yu, Yong, Zhu, Zhexin, Wang, Juexuan, Kolodziejczyk, Aleksandra A., Campos, Lia S., Wang, Cui, Yang, Fengtang, Zhong, Zhen, Fu, Beiyuan, Eckersley-Maslin, Melanie A., Woods, Michael, Tanaka, Yosuke, Chen, Xi, Wilkinson, Adam C., Bussell, James, White, Jacqui, Ramirez-Solis, Ramiro, Reik, Wolf, Göttgens, Berthold, Teichmann, Sarah A., Tam, Patrick P. L., Nakauchi, Hiromitsu, Zou, Xiangang, Lu, Liming, and Liu, Pentao

Subjects
Stem cells -- Health aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Jian Yang [1]; David J. Ryan [1]; Wei Wang [1]; Jason Cheuk-Ho Tsang [1]; Guocheng Lan [2]; Hideki Masaki [3]; Xuefei Gao [1]; Liliana Antunes [1]; Yong Yu [1]; [...]

Published
2017
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8. Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming

Author

Yang, Jian, Rajan, Sandeep S, Friedrich, Mathias J, Lan, Guocheng, Zou, Xiangang, Ponstingl, Hannes, Garyfallos, Dimitrios A, Liu, Pentao, Bradley, Allan, Metzakopian, Emmanouil, Bradley, Allan [0000-0002-2349-8839], and Apollo - University of Cambridge Repository

Subjects
Pluripotent Stem Cells, epiblast stem cells, induced pluripotent stem cells, genome-wide screen, Gene Dosage, Article, CRISPR screen, Cell Line, Mice, Animals, Humans, Clustered Regularly Interspaced Short Palindromic Repeats, lcsh:QH301-705.5, CRISPR/Cas9, reprogramming pathways, lcsh:R5-920, gain-of-function, CRISPR activation, activation screen, reprogramming, Cellular Reprogramming, lcsh:Biology (General), embryonic structures, CRISPR-Cas Systems, lcsh:Medicine (General), Octamer Transcription Factor-3, Biomarkers, Germ Layers, Genome-Wide Association Study, Transcription Factors
Abstract

Summary Primed epiblast stem cells (EpiSCs) can be reverted to a pluripotent embryonic stem cell (ESC)-like state by expression of single reprogramming factor. We used CRISPR activation to perform a genome-scale, reprogramming screen in EpiSCs and identified 142 candidate genes. Our screen validated a total of 50 genes, previously not known to contribute to reprogramming, of which we chose Sall1 for further investigation. We show that Sall1 augments reprogramming of mouse EpiSCs and embryonic fibroblasts and that these induced pluripotent stem cells are indeed fully pluripotent including formation of chimeric mice. We also demonstrate that Sall1 synergizes with Nanog in reprogramming and that overexpression in ESCs delays their conversion back to EpiSCs. Lastly, using RNA sequencing, we identify and validate Klf5 and Fam189a2 as new downstream targets of Sall1 and Nanog. In summary, our work demonstrates the power of using CRISPR technology in understanding molecular mechanisms that mediate complex cellular processes such as reprogramming., Graphical Abstract, Highlights • Genome-scale CRISPRa screen in mouse EpiSCs identifies novel reprogramming factors • 50 novel genes, including Sall1 and Fam189a2, identified to mediate reprogramming • Sall1 synergizes with Nanog to increase reprogramming efficiency in EpiSCs and MEFs • RNA-seq provides insight into downstream pathways of Sall1 and Nanog-mediated reprogramming, In this study, Metzakopian and colleagues use CRISPR activation to perform a genome-scale reprogramming screen on mouse epiblast stem cells identifying 50 novel candidates. In addition, the authors provide evidence of Sall1 being a potent reprogramming gene, capable of synergizing with Nanog. Lastly, using RNA-seq, the authors provide insight into potential downstream targets of Sall1 and Nanog.

Published
2019

9. Establishment of porcine and human expanded potential stem cells

Author

Gao, Xuefei, Nowak-imialek, Monika, Chen, Xi, Chen, Dongsheng, Herrmann, Doris, Ruan, Degong, Chen, Andy Chun Hang, Eckersley-maslin, Melanie A., Ahmad, Shakil, Lee, Yin Lau, Kobayashi, Toshihiro, Ryan, David, Zhong, Jixing, Zhu, Jiacheng, Wu, Jian, Lan, Guocheng, Petkov, Stoyan, Yang, Jian, Antunes, Liliana, Campos, Lia S., Fu, Beiyuan, Wang, Shengpeng, Yong, Yu, Wang, Xiaomin, Xue, Song-guo, Ge, Liangpeng, Liu, Zuohua, Huang, Yong, Nie, Tao, Li, Peng, Wu, Donghai, Pei, Duanqing, Zhang, Yi, Lu, Liming, Yang, Fengtang, Kimber, Susan J., Reik, Wolf, Zou, Xiangang, Shang, Zhouchun, Lai, Liangxue, Surani, Azim, Tam, Patrick P. L., Ahmed, Asif, Yeung, William Shu Biu, Teichmann, Sarah A., Niemann, Heiner, Liu, Pentao, Gao, Xuefei, Nowak-imialek, Monika, Chen, Xi, Chen, Dongsheng, Herrmann, Doris, Ruan, Degong, Chen, Andy Chun Hang, Eckersley-maslin, Melanie A., Ahmad, Shakil, Lee, Yin Lau, Kobayashi, Toshihiro, Ryan, David, Zhong, Jixing, Zhu, Jiacheng, Wu, Jian, Lan, Guocheng, Petkov, Stoyan, Yang, Jian, Antunes, Liliana, Campos, Lia S., Fu, Beiyuan, Wang, Shengpeng, Yong, Yu, Wang, Xiaomin, Xue, Song-guo, Ge, Liangpeng, Liu, Zuohua, Huang, Yong, Nie, Tao, Li, Peng, Wu, Donghai, Pei, Duanqing, Zhang, Yi, Lu, Liming, Yang, Fengtang, Kimber, Susan J., Reik, Wolf, Zou, Xiangang, Shang, Zhouchun, Lai, Liangxue, Surani, Azim, Tam, Patrick P. L., Ahmed, Asif, Yeung, William Shu Biu, Teichmann, Sarah A., Niemann, Heiner, and Liu, Pentao

Abstract

We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.

Published
2019

14. A de novo silencer causes elimination of MITF-M expression and profound hearing loss in pigs

Author

Chen, Lei, primary, Guo, Weiwei, additional, Ren, Lili, additional, Yang, Mingyao, additional, Zhao, Yaofeng, additional, Guo, Zongyi, additional, Yi, Haijin, additional, Li, Mingzhou, additional, Hu, Yiqing, additional, Long, Xi, additional, Sun, Boyuan, additional, Li, Jinxiu, additional, Zhai, Suoqiang, additional, Zhang, Tinghuan, additional, Tian, Shilin, additional, Meng, Qingyong, additional, Yu, Ning, additional, Zhu, Dan, additional, Tang, Guoqing, additional, Tang, Qianzi, additional, Ren, Liming, additional, Liu, Ke, additional, Zhang, Shihua, additional, Che, Tiandong, additional, Yu, Zhengquan, additional, Wu, Nan, additional, Jing, Lan, additional, Zhang, Ran, additional, Cong, Tao, additional, Chen, Siqing, additional, Zhao, Yiqiang, additional, Zhang, Yue, additional, Bai, Xiaoqing, additional, Guo, Ying, additional, Zhao, Lidong, additional, Zhang, Fengming, additional, Zhao, Hui, additional, Zhang, Liang, additional, Hou, Zhaohui, additional, Zhao, Jiugang, additional, Li, Jianan, additional, Zhang, Lijuan, additional, Sun, Wei, additional, Zou, Xiangang, additional, Wang, Tao, additional, Ge, Liangpeng, additional, Liu, Zuohua, additional, Hu, Xiaoxiang, additional, Wang, Jingyong, additional, Yang, Shiming, additional, and Li, Ning, additional

Published
2016
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16. Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells.

Author

Espana-Agusti, Judit, Zou, Xiangang, Wong, Kim, Fu, Beiyuan, Yang, Fengtang, Tuveson, David A., Adams, David J., and Matakidou, Athena

Subjects
*EPITHELIAL cells, *KIDNEY physiology, *GENE targeting, *LABORATORY mice, *SPATIOTEMPORAL processes
Abstract

Genetically relevant mouse models need to recapitulate the hallmarks of human disease by permitting spatiotemporal gene targeting. This is especially important for replicating the biology of complex diseases like cancer, where genetic events occur in a sporadic fashion within developed somatic tissues. Though a number of renal tubule targeting mouse lines have been developed their utility for the study of renal disease is limited by lack of inducibility and specificity. In this study we describe the generation and characterisation of two novel mouse lines directing CreERT2 expression to renal tubular epithelia. The Pax8-CreERT2 transgenic line uses the mouse Pax8 promoter to direct expression of CreERT2 to all renal tubular compartments (proximal and distal tubules as well as collecting ducts) whilst the Slc22a6-CreERT2 knock-in line utilises the endogenous mouse Slc22a6 locus to specifically target the epithelium of proximal renal tubules. Both lines show high organ and tissue specificity with no extrarenal activity detected. To establish the utility of these lines for the study of renal cancer biology, Pax8-CreERT2 and Slc22a6-CreERT2 mice were crossed to conditional Vhl knockout mice to induce long-term renal tubule specific Vhl deletion. These models exhibited renal specific activation of the hypoxia inducible factor pathway (a VHL target). Our results establish Pax8-CreERT2 and Slc22a6-CreERT2 mice as valuable tools for the investigation and modelling of complex renal biology and disease. [ABSTRACT FROM AUTHOR]

Published
2016
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17. Heavy chain–only antibodies are spontaneously produced in light chain–deficient mice

Author

Zou, Xiangang, primary, Osborn, Michael J., additional, Bolland, Daniel J., additional, Smith, Jennifer A., additional, Corcos, Daniel, additional, Hamon, Maureen, additional, Oxley, David, additional, Hutchings, Amanda, additional, Morgan, Geoff, additional, Santos, Fatima, additional, Kilshaw, Peter J., additional, Taussig, Michael J., additional, Corcoran, Anne E., additional, and Brüggemann, Marianne, additional

Published
2007
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