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Indolocarbazole (ICZ) alkaloid constitutes a group of natural products which manifested powerful biological activity, especially anticancer. However, it was laborious to find ICZs-yield organism when anticancer activity was adopted as selection target. The specificity of 6 primer pairs was detected and the results revealed that primer rebBN1/rebBC1 possessed preferable specificity because indolocarbazole-producers DNA was exclusively amplified and no target fragment was amplified from any of indolocarbazole nonproducers. Therefore, primer rebBN1/rebBC1 could specifically identify indolocarbazole-producers from microorganism strains. Key words: Indolocarbazole, rebBN1/rebBC1, ICZs-producing microorganism screening.

 Actinomycetes represent a valuable source of commercially significant antibiotics. However, the yields of antibiotics in wild Actinomycetes usually could not meet the commercial needs and so antibiotics yield improvement is a constant requirement in the drug industry. Various methods and strategies such as traditional strain breeding methods and fermentation optimization methods have been tried and some new ideas such as gene engineering, mixed culture and quorum sensing along with empirical approaches are still on the way to gain this ultimate goal. This review will focus on the strategies and approaches for Actinomycetes antibiotics augment.Â

The known or suspected hazards of polycyclic aromatic hydrocarbons (PAHs) have provoked enormous concentration and endeavours to relieve or eliminate these precarious compounds from miscellaneous environments including soil, water and air. Among various interventions, biodegradation is an appealing approach for its comparative high efficiency and preferable safety. Microorganisms played crucial role in biodegradation of PAHs. Traditional access mainly including culture-dependent procedures has discovered and isolated PAHs-degrading microorganisms which could be subsequently applied to specific contaminated locus. Although certain progress has been achieved owing to traditional methods, much details in PAHs bioremedation leave pending because of the complexity nature of this process. As the rapid development of biology, molecular techniques such as PCR, fingerprinting technique (mainly DGGE), DNA hybridization technique and gene reporters technique have been intensively applied to gain further insight into the mechanism of PAHs degradation. These techniques not only proved the existence and role of uncultivable microorganisms in the whole population of PAHs degrading related microbials, but also made it possible to revealed the otherwise undetectable complex relationships between multi-microorganism concerned in PAHs biodegradation. Application of such techniques in the field of PAHs biodegradation were reviewed in this article.