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1. IMPLEMENTING GENOMIC SELECTION IN THE IMB: CHALLENGES AND OPPORTUNITIES.

Author

Biffani, Stefano, Gómez, Mayra, Cimmino, Roberta, Rossi, Dario, Zullo, Gianluigi, Negrini, Riccardo, Cesarani, Alberto, Campanile, Giuseppe, and Neglia, Gianluca

Subjects
WATER buffalo, GENOTYPES, MOZZARELLA cheese
Abstract

Copyright of Revista Cientifica de la Facultade de Veterinaria is the property of Universidad del Zulia, Facultad de Ciencias Veterinarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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7. Natural antioxidants during in vitro culture improve embryo quality in cattle

Author

Zullo, Gianluigi and Zullo, Gianluigi

Abstract

In cattle, in vitro-produced (IVP) bovine embryos have been around in large numbers for over two decades. It is probably fair to say that their main use has been in research, as a means of understanding what happens during normal in vivo embryo development in cattle, as stepping stones to other technologies, such as nuclear transfer, and as a tool for examining what goes wrong in development following such procedures, and as models from which to extrapolate findings to other species. The high incidence of developmental failure in the in vitro embryo production system has been also attributed to suboptimal culture conditions that induce oxidative stress. Oxidative stress has been implicated in many different types of cell injuries, including membrane lipids peroxidation, oxidation of amino acids and nucleic acids, apoptosis and necrosis which may subsequently decrease the viability of IVP embryos. To overcome this, exogenous antioxidant compounds have been frequently used to increase antioxidant capacity of embryos via increasing intracellular levels of reactive oxygen species (ROS) scavengers. Development of accurate laboratory methods to assess embryo quality will improve the efficiency of embryo production from in-vitro culture systems. Currently, the techniques of TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL) for the detection of apoptosis, and differential staining for determining the ratio of inner cell mass (ICM) to trophectoderm (TE) cells, are used separately to assess embryo quality in a range of different species. Furthermore, the ability to cryopreserve embryos, without critical loss of viability, has a profound effect on the success of assisted reproductive techniques. The survival of cryopreserved in vitro produced (IVP) embryos, as measured either by post-warming survival in culture or by established pregnancies after embryo transfer, has lagged behind that of in vivo-derived embryos. Even if the background of thi

Published
2015

8. Early Prediction of Corpus Luteum Functionality Using an Imaging Software

Author

Francesca Licitra, M. Russo, G. Zullo, Gerardo Fatone, Giuseppe Campanile, Angela Salzano, Giuseppe Anglani, Alessio Cotticelli, Salzano, Angela, Russo, Marco, Anglani, Giuseppe, Licitra, Francesca, Zullo, Gianluigi, Cotticelli, Alessio, Fatone, Gerardo, and Campanile, Giuseppe

Subjects
blood flow area, 040301 veterinary sciences, medicine.medical_treatment, Luteal phase, progesterone, corpus luteum, buffaloes, blood flow area, progesterone, pregnancy diagnosis, 0403 veterinary science, corpus luteum, 03 medical and health sciences, Animal science, pregnancy diagnosis, buffaloes, Medicine, 030304 developmental biology, Original Research, Estrous cycle, 0303 health sciences, Pregnancy, lcsh:Veterinary medicine, General Veterinary, business.industry, Artificial insemination, Repeated measures design, 04 agricultural and veterinary sciences, Blood flow, medicine.disease, medicine.anatomical_structure, lcsh:SF600-1100, Veterinary Science, Analysis of variance, business, Corpus luteum
Abstract

The present study aimed to assess the applicability of luteal blood flow data acquired through the use of color Doppler ultrasonography and a post-processing analysis tool (ImageJ) for predicting pregnancy in buffaloes (Bubalus bubalis). The experiment was carried out on 59 multiparous Italian Mediterranean buffaloes that underwent synchronization of estrus and fixed-time artificial insemination (TAI). Corpus luteum features (size: CLS and blood flow: BFA) were taken from Day 5 to 10 after TAI and retrospectively measured with ImageJ. In the same period, blood samples were taken to assess progesterone (P4) concentrations. Pregnancy diagnosis was carried out on Day 45 by ultrasound and confirmed on Day 70 post-TAI. Differences in CLS, BFA, and P4 concentrations from Day 5 to 10 after TAI measured between groups were analyzed by ANOVA repeated measures as were differences within each day of measuring. Buffaloes that established a pregnancy (n = 29; 55%) had larger CLS (2.2 ± 0.1 vs. 1.9 ± 0.1 cm2; P < 0.01), higher BFA (0.6 ± 0.0 vs. 0.4 ± 0.0 cm2; P < 0.01), and higher P4 blood level (1.8 ± 0.1 vs. 1.4 ± 0.1; P < 0.01) during Day 5–10 as compared to not-pregnant buffaloes (n = 22). Throughout the entire period, the first feature that changed between groups was P4 blood concentration at Day 7 (1.7 ± 0.1 vs. 1.2 ± 0.1; P < 0.05) followed by BFA at Day 8 (0.6 ± 0.0 vs. 0.5 ± 0.0; P < 0.05), respectively, in pregnant and not-pregnant animals. The ROC analyses indicated that P4 was able to predict pregnancy since Day 5 (P < 0.05) although a more reliable result could be obtained from Day 8 (P < 0.01). At Day 10, it was possible to set a cutoff value for every parameter taken into account. The logistic regression analysis showed that pregnancy was positively influenced by P4 concentration (odds ratio 534.127; P < 0.01) and BFA (odds ratio 744.893; P < 0.01). In conclusion, the use of color Doppler ultrasonography, together with ImageJ, identified different patterns of BFA between pregnant and not-pregnant buffaloes starting from Day 8 post-TAI.

Published
2020

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