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Molecular chaperones such as Hsp40 and Hsp70 hold the androgen receptor (AR) in an inactive conformation. They are released in the presence of androgens, enabling transactivation and causing the receptor to become aggregation-prone. Here we show that these molecular chaperones recognize a region of the AR N-terminal domain (NTD), including a FQNLF motif, that interacts with the AR ligand-binding domain (LBD) upon activation. This suggests that competition between molecular chaperones and the LBD for the FQNLF motif regulates AR activation. We also show that, while the free NTD oligomerizes, binding to Hsp70 increases its solubility. Stabilizing the NTD-Hsp70 interaction with small molecules reduces AR aggregation and promotes its degradation in cellular and mouse models of the neuromuscular disorder spinal bulbar muscular atrophy. These results help resolve the mechanisms by which molecular chaperones regulate the balance between AR aggregation, activation and quality control.

SBAS-InSAR technology is effective in obtaining surface deformation information and is widely used in monitoring landslides and mining subsidence. However, SBAS-InSAR technology is susceptible to various errors, including atmospheric, orbital, and phase unwrapping errors. These multiple errors pose significant challenges to precise deformation monitoring over large areas. This paper examines the spatial characteristics of these errors and introduces a spatially constrained SBAS-InSAR method, termed SSBAS-InSAR, which enhances the accuracy of wide-area surface deformation monitoring. The method employs multiple stable ground points to create a control network that limits the propagation of multiple types of errors in the interferometric unwrapped data, thereby reducing the impact of long-wavelength signals on local deformation measurements. The proposed method was applied to Sentinel-1 data from parts of Jining, China. The results indicate that, compared to the traditional SBAS-InSAR method, the SSBAS-InSAR method significantly reduced phase closure errors, deformation rate standard deviations, and phase residues, improved temporal coherence, and provided a clearer representation of deformation in time-series curves. This is crucial for studying surface deformation trends and patterns and for preventing related disasters. [ABSTRACT FROM AUTHOR]

Since 2008, annual outbreaks of green tides in the Yellow Sea have had severe impacts on tourism, fisheries, water sports, and marine ecology, necessitating effective interception and removal measures. Satellite remote sensing has emerged as a promising tool for monitoring large-scale green tides due to its wide coverage and instantaneous imaging capabilities. Additionally, drift prediction techniques can forecast the location of future green tides based on remote sensing monitoring information. This monitoring and prediction information is crucial for developing an effective plan to intercept and remove green tides. One key aspect of this monitoring information is the green tide distribution envelope, which can be generated automatically and quickly using buffer analysis methods. However, this method produces a large number of envelope vertices, resulting in significant computational burden during prediction calculations. To address this issue, this paper proposes a simplification method based on azimuth difference and side length (SM-ADSL). Compared to the isometric and Douglas–Peucker methods with the same simplification rate, SM-ADSL exhibits better performance in preserving shape and area. The simplified distribution envelope can shorten prediction times and enhance the efficiency of emergency decision-making for green tide disasters. [ABSTRACT FROM AUTHOR]

Lutein is widely used in medicine, health care, and food processing due to its antioxidant effects; however, it is difficult for the traditional extraction of lutein using marigolds to meet the increasing market demand for lutein. To achieve high-efficiency lutein production, we investigated the effects of different conditions on the biomass accumulation and lutein yield of Chlorella vulgaris. The optimized cultivation conditions include mixotrophic cultivation using sodium nitrate as a nitrogen source, maintaining a total-organic-carbon-to-total-nitrogen ratio of 12:1, a total-nitrogen-to-total-phosphorus ratio of 10:1, and lighting duration of 24 h. The results of the study indicated that under these specific conditions, Chlorella vulgaris attained a final biomass concentration, biomass productivity, and growth yield of 6.08 g·L−1, 1.00 g·L−1·d−1, and 1.67 g biomass/g TOC, respectively. Additionally, the concentrations of total chlorophyll, carotenoid, lutein, and protein reached 139.20 mg·L−1, 31.87 mg·L−1, 15.02 mg·L−1, and 2.17 g·L−1, respectively, and the content of lutein reached 2.47 mg·g−1. This study supplies a theoretical basis for the industrial application of lutein production using Chlorella vulgaris. [ABSTRACT FROM AUTHOR]

Purpose: Periductal mastitis (PDM) is a chronic inflammatory lesion of the breast with an unknown etiology, and it is difficult for clinicians to differentiate it from granulomatous lobular mastitis (GLM), although they have different treatment strategies and prognosis. This study aimed to investigate the differences in their clinicopathologic features to inform treatment strategies. Patients and Methods: Between 2011 and 2020, 121 patients diagnosed with PDM and 57 patients with GLM were retrospective analysis. Patient data were extracted on demographics, clinical presentation, pathologic characteristics, treatments and clinical response. Histopathological evaluations were performed on core needle biopsy specimens. Immunohistochemical stains using antibodies against CD3, CD4, CD8, CD20, and CD138 was performed to define immune cell infiltration. Results: PDM patients had a higher median age compared to GLM patients (38 vs 32, p< 0.001). PDM was primarily located in the areolar area, while GLM predominantly affected the peripheral quadrant of the breast (56.20% vs 75.44%, p< 0.001). Histopathologically, more ductal dilatation (90.08% vs 3.51%, p< 0.001), ductal wall thickening (47.93% vs 1.75%, p< 0.001), and ductal rupture (44.63% vs 5.26%, p< 0.001) were observed in PDM. GLM presented with significantly more granuloma (94.74% vs 10.74%, p< 0.001), microabscess (68.42% vs 28.93%, p< 0.001), and lipid vacuole (40.35% vs 8.26%, p< 0.001) formation than PDM. Immunohistochemical analysis revealed a significant presence of CD20+ B lymphocytes in PDM and a higher prevalence of CD8+ T lymphocytes in GLM, indicating differing immune responses. Treatment outcomes varied, with PDM patients responding well to surgery and anti-mycobacterial therapy, while GLM patients showed favorable responses to steroid therapy. Conclusion: PDM is a specific entity with a similar clinical presentation but distinct histopathological features and immune profiles to GLM. Further research is needed to elucidate the pathogenesis and optimize therapeutic approaches for these breast inflammatory conditions. [ABSTRACT FROM AUTHOR]