124 results on '"Xiuying, Yang"'
Search Results
2. Comparison between an SGLT2 inhibitor and insulin in tumor-to-tissue contrasts in 18F-FDG PET imaging of diabetic mice
- Author
-
Meidai, Liang, Yujing, Du, Zhaoyu, Liu, Shanshi, Li, Guangyu, Zhao, Yan, Fan, Xiuying, Yang, and Jianhua, Zhang
- Published
- 2023
- Full Text
- View/download PDF
3. Impact of the COVID-19 lockdown on air pollution in an industrial city in Northeastern China
- Author
-
Xueyan Wang, Yuqing Wang, Yiran Zhao, Meijin Tian, Xinmeng Wu, Yongzhen Zhang, Feng Gao, Junjiao Sun, Xiaoqing Wei, Xiuying Yang, and Xiaohan Qu
- Subjects
novel coronavirus ,air quality index ,spatiotemporal distribution ,lockdown ,particulate matter ,nitrogen dioxide ,Environmental engineering ,TA170-171 - Abstract
Many studies in China investigated how the lockdown following the coronavirus disease 2019 substantially affected air quality; however, few were conducted in Northeastern China. Here, the changes in six criteria air pollutants, including particulate matter (PM10 and PM2.5), nitrogen dioxide (NO2), carbon monoxide (CO), sulfur dioxide (SO2), and ozone (O3), were investigated in Shenyang from January to May 2015–2020. Compared with the pre-lockdown, the mass concentrations of PM2.5, PM10, SO2, NO2, and CO during the lockdown decreased by 40.3% to 48.6%, indicating a positive impact of lockdown policies on reducing pollutant emissions. The responses of PM2.5, PM10, and CO to the lockdown measures in downtown areas were more sensitive than in the suburbs. However, the O3 concentration showed the opposite trend, attributed to the drop in NOx and particulate matters. Compared to the same period in 2015–2019, the proportion of days with good air quality increased from 63.2% to 77.2% during the lockdown and Shenyang experienced no severe pollution. Our results suggest that reducing human activities can improve air quality; however, coordinated control policies of O3, PM2.5, and NO2 are imperative.
- Published
- 2023
- Full Text
- View/download PDF
4. Kaempferol attenuates nonalcoholic fatty liver disease in type 2 diabetic mice via the Sirt1/AMPK signaling pathway
- Author
-
Na Li, Lin Yin, Jiamin Shang, Meidai Liang, Zhaoyu Liu, Haiguang Yang, Guifen Qiang, Guanhua Du, and Xiuying Yang
- Subjects
Kaempferol ,NAFLD ,Lipid accumulation ,Sirt1 ,AMPK ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases with limited treatment options. Moreover, its prevalence is doubled in type 2 diabetes mellitus (T2DM). Kaempferol (KAP) is a flavonoid compound that has been suggested to have beneficial effects on NAFLD, but studies on the mechanism are lacking, especially in the diabetic state. Herein, we investigated the effect of KAP on NAFLD associated with T2DM and its underlying mechanism in vitro and in vivo. The results of in vitro studies indicated that KAP treatment (10−8–10−6 M) significantly reduced lipid accumulation in oleic acid-induced HepG2 cells. Moreover, in the T2DM animal model of db/db mice, we confirmed that KAP (50 mg/kg) significantly reduced lipid accumulation and improved liver injury. Mechanistic studies in vitro and in vivo showed that Sirtuin 1 (Sirt1)/AMP-activated protein kinase (AMPK) signal was involved in KAP regulation of hepatic lipid accumulation. KAP treatment activated Sirt1 and AMPK, upregulated the levels of fatty acid oxidation-related protein proliferator activated receptor gamma coactivator 1α (PGC1α); and downregulated lipid synthesis-related proteins, including acetyl-coA carboxylase (ACC), fatty acid synthase (FASN), and sterol regulatory element-binding protein 1 (SREBP1). Furthermore, the curative effect of KAP on lipid accumulation was abolished by siRNA-mediated knockdown of either Sirt1 or AMPK. Collectively, these findings suggest that KAP may be a potential therapeutic agent for NAFLD associated with T2DM by regulating hepatic lipid accumulation through activation of Sirt1/AMPK signaling.
- Published
- 2023
- Full Text
- View/download PDF
5. Berbamine inhibits RANKL- and M-CSF-mediated osteoclastogenesis and alleviates ovariectomy-induced bone loss
- Author
-
Guobin Qi, Zengxin Jiang, Wei Lu, Defang Li, Weibing Chen, Xiuying Yang, Lei Ding, and Hengfeng Yuan
- Subjects
berbamine ,osteoclastogenesis ,osteoporosis ,NF-κB ,MAPK ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Osteoporosis is a common public health problem characterized by decreased bone mass, increased bone brittleness and damage to the bone microstructure. Excessive bone resorption by osteoclasts is the main target of the currently used drugs or treatment for osteoporosis. Effective antiresorptive drugs without side effects following long-term administration have become a major focus of anti-osteoporotic drugs. In the present study, we investigated the effect of berbamine, a small molecule natural product from Berberis amurensis Rupr, a traditional Chinese medicine, on RANKL-induced osteoclast differentiation in vitro and ovariectomy-induced bone loss in vivo. The results demonstrated that berbamine at a safe and effective dose inhibited osteoclastogenesis and bone resorption function in vitro by suppressing the nuclear factor-κB signaling pathway. In addition, berbamine protected against osteoporosis by inhibiting osteoclastogenesis and bone resorption function without affecting osteogenesis in the ovariectomy mouse model. These findings revealed that berbamine has a protective role against osteoporosis and may represent a novel promising treatment strategy for osteoporosis.
- Published
- 2022
- Full Text
- View/download PDF
6. Omaveloxolone inhibits IL-1β-induced chondrocyte apoptosis through the Nrf2/ARE and NF-κB signalling pathways in vitro and attenuates osteoarthritis in vivo
- Author
-
Zengxin Jiang, Guobin Qi, Wei Lu, Hao Wang, Defang Li, Weibin Chen, Lei Ding, Xiuying Yang, Hengfeng Yuan, and Qingmin Zeng
- Subjects
osteoarthritis ,omaveloxolone ,Nrf2 ,NF-κB ,apoptosis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Osteoarthritis (OA) is a common degenerative joint disease. Effective drugs that can halt or decelerate osteoarthritis progression are still lacking. Omaveloxolone is a semisynthetic oleanane triterpenoid exerting antioxidative and anti-inflammatory effects. The present study aims to determine whether omaveloxolone has a therapeutic effect on OA. Chondrocytes were treated with interleukin (IL)-1β to establish an OA cell model in vitro. Indicators of cell viability, oxidative stress, inflammation, cell apoptosis and extracellular matrix (ECM) degradation were investigated. Proteins related to the Nuclear factor erythroid derived-2-related factor 2 (Nrf2)/antioxidant response element (ARE) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signalling pathways were assessed using Western blotting. A destabilized medial meniscus surgery-induced OA rat model was used in vivo. Gait analysis, microcomputed tomography analysis, and histopathological and immunohistochemical analyses were performed to determine the therapeutic effect of omaveloxolone on attenuating osteoarthritis in vivo. The results showed that omaveloxolone exerts antioxidative, anti-inflammatory, antiapoptotic and anti-ECM degradation effects via activation of the Nrf2/ARE signalling pathway and inhibition of the NF-κB signalling pathway in chondrocytes in vitro and attenuates OA progression in vivo, suggesting that omaveloxolone may be a potential therapeutic agent for OA.
- Published
- 2022
- Full Text
- View/download PDF
7. Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung
- Author
-
Zijing Wang, Peng Ma, Yisa Wang, Biyu Hou, Can Zhou, He Tian, Bowen Li, Guanghou Shui, Xiuying Yang, Guifen Qiang, Chengqian Yin, and Guanhua Du
- Subjects
Medicine ,Science - Abstract
Abstract Endoplasmic reticulum (ER) stress is a cellular state that results from the overload of unfolded/misfolded protein in the ER that, if not resolved properly, can lead to cell death. Both acute lung infections and chronic lung diseases have been found related to ER stress. Yet no study has been presented integrating metabolomic and transcriptomic data from total lung in interpreting the pathogenic state of ER stress. Total mouse lungs were used to perform LC–MS and RNA sequencing in relevance to ER stress. Untargeted metabolomics revealed 16 metabolites of aberrant levels with statistical significance while transcriptomics revealed 1593 genes abnormally expressed. Enrichment results demonstrated the injury ER stress inflicted upon lung through the alteration of multiple critical pathways involving energy expenditure, signal transduction, and redox homeostasis. Ultimately, we have presented p-cresol sulfate (PCS) and trimethylamine N-oxide (TMAO) as two potential ER stress biomarkers. Glutathione metabolism stood out in both omics as a notably altered pathway that believed to take important roles in maintaining the redox homeostasis in the cells critical for the development and relief of ER stress, in consistence with the existing reports.
- Published
- 2021
- Full Text
- View/download PDF
8. Antagonizing urotensin receptor is a novel therapeutic strategy for glucocorticoid‐induced skeletal muscle atrophy
- Author
-
Lin Yin, Na Li, Weihua Jia, Nuoqi Wang, Meidai Liang, Jiamin Shang, Guifen Qiang, Guanhua Du, and Xiuying Yang
- Subjects
Medicine (General) ,R5-920 - Published
- 2022
- Full Text
- View/download PDF
9. Versatile Solid Modifications of Multicomponent Pharmaceutical Salts: Novel Metformin–Rhein Salts Based on Advantage Complementary Strategy Design
- Author
-
Mingchao Yu, Meidai Liang, Qi An, Wenwen Wang, Baoxi Zhang, Shiying Yang, Jian Zhou, Xiuying Yang, Dezhi Yang, Li Zhang, Guanhua Du, and Yang Lu
- Subjects
drug–drug salt ,polymorphism ,solubility ,hygroscopicity ,hypoglycemic activity ,diabetic complications ,Pharmacy and materia medica ,RS1-441 - Abstract
This study aimed to develop an effective treatment for diabetes and diabetic complications, based on the advantage complementary strategy of drug–drug salt, by designing and synthesizing the multicomponent molecular salts containing metformin (MET) and rhein (RHE). Finally, the salts of MET–RHE (1:1), MET–RHE–H2O (1:1:1), MET–RHE–ethanol–H2O (1:1:1:1), and MET–RHE–acetonitrile (2:2:1) were obtained, indicating the polymorphism of salts formed by MET and RHE. The structures were analyzed by the combination of characterization experiments and theoretical calculation, and the formation mechanism of polymorphism was discussed. The obtained results of in vitro evaluation showed that MET–RHE had a similar hygroscopicity with metformin hydrochloride (MET·HCl), and the solubility of the component of RHE increased by approximately 93 times, which laid a foundation for improving the bioavailability of MET and RHE in vivo. The evaluation of hypoglycemic activity in mice (C57BL/6N) indicated that MET–RHE exhibited better hypoglycemic activity than the parent drugs and the physical mixtures of MET and RHE. The above findings demonstrate that this study achieved the complementary advantages of MET and RHE through the multicomponent pharmaceutical salification technique, and provides new possibilities for the treatment of diabetic complications.
- Published
- 2023
- Full Text
- View/download PDF
10. Mechanical Properties of Fast-Growing Poplar Reinforced with Carbon Fiber
- Author
-
Ying Gao, Liwei Guo, Xiao Zhang, Yuzhuo Wang, Xiuying Yang, ChuanGuo Fu, and Ziqing Liu
- Subjects
Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
This paper presents mechanical properties of fast-growing poplar specimens reinforced with carbon fibers. A total of 90 specimens including 10 contrast specimens were tested to investigate the influence from the following parameters: (a) different carbon fiber ratios (0.167%, 0.251%, 0.334%, 0.401%, and 0.501%) and (b) different fiber locations. The failure mode, compressive strength, elastic modulus, and axial deformation of specimens were analyzed. The test results indicate the following: (1) The compressive strength, elastic modulus, and axial deformation of specimens reinforced with carbon fiber were significantly improved compared with that of fast-growing poplar specimens. The compressive strength, elastic modulus, and axial deformation increased by 54.1–76.03%, 11.58–22.89%, and 24.86–60.06%, respectively. (2) There was little effect on the compressive strength of the specimen with the increase of carbon fiber ratio. With the increase of carbon fiber ratio, the elastic modulus of specimens slightly decreased and the axial deformation increased. The elastic modulus decreased by 1.39–18.69%, and the axial deformation increased by 10%–48%. (3) The different locations of the carbon fiber distribution resulted in a large difference in the compressive strength of the specimens, while the effects on the modulus of elasticity and axial deformation were not significant. Finally, the compressive strength calculation formula was proposed.
- Published
- 2022
- Full Text
- View/download PDF
11. Combination of Docking-Based and Pharmacophore-Based Virtual Screening Identifies Novel Agonists That Target the Urotensin Receptor
- Author
-
Na Li, Lin Yin, Xi Chen, Jiamin Shang, Meidai Liang, Li Gao, Guifen Qiang, Jie Xia, Guanhua Du, and Xiuying Yang
- Subjects
urotensin receptor ,urotensin-II ,docking-based virtual screening ,pharmacophore-based virtual screening ,drug screening ,Organic chemistry ,QD241-441 - Abstract
The urotensin receptor (UT receptor), a G-protein-coupled receptor mediating urotensin-II and urotensin-II-related peptide signaling in the urotensinergic system, has multiple pharmacological activities. However, there is no drug targeting the UT receptor currently in clinical use, and the discovery of new leads is still important. The complete crystal structure of the UT receptor has not yet been resolved and a screening strategy combining multiple methods can improve the accuracy and efficiency of drug screening. This study aimed to identify novel UT receptor agonists using a combination of docking-based, pharmacophore-based, and cell-based drug screening. First, the three-dimensional structures of the UT receptor were constructed through single-template, multi-template homologous modeling and threading strategies. After structure evaluation and ligand enrichment analysis, a model from the threading modeling was selected for docking-based virtual screening based on stepwise filtering, and 1368 positive compounds were obtained from our compound library. Second, the pharmacophore models were constructed using known ligands targeting the UT receptor for pharmacophore-based virtual screening. A model was selected after model validation, and 300 positive compounds were retrieved. Then, after intersecting the results of two different virtual screening methods with 570 compound entities from our primary screening, 14 compounds were obtained. Finally, three hits were obtained after in vitro confirmation. Furthermore, preliminary evaluation of the hits showed that they influenced glucose consumption. In summary, by integrating docking-based, pharmacophore-based, and in vitro drug screening, three new agonists targeting the UT receptor were identified which may serve as promising therapeutic agents for urotensinergic system disorders.
- Published
- 2022
- Full Text
- View/download PDF
12. Puerarin ameliorates skeletal muscle wasting and fiber type transformation in STZ-induced type 1 diabetic rats
- Author
-
Lin Yin, Xi Chen, Na Li, Weihua Jia, Nuoqi Wang, Biyu Hou, Haiguang Yang, Li Zhang, Guifen Qiang, Xiuying Yang, and Guanhua Du
- Subjects
Puerarin ,Type 1 diabetes ,Skeletal muscle wasting ,Rats ,Protein synthesis ,Muscle type transformation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Puerarin is an isoflavonoid extracted from Pueraria lobate with extensive pharmacological effects in traditional Chinese medicine. The evidence implicates that puerarin mitigates hyperglycemia and various relevant complications. Here, the effect of puerarin on skeletal muscle wasting induced by type 1 diabetes (T1D) was explored. Streptozotocin (STZ)-induced T1D male Sprague Dawley (SD) rats were used in this study. Muscle strength, weight and size were measured. L6 rat skeletal muscle cells were applied for in vitro study. Our results showed that eight-week oral puerarin administration (100 mg/kg) increased muscle strengths and weights accompanied by enhanced skeletal muscle cross-sectional areas in diabetic rats. Simultaneously, puerarin also reduced expressions of several muscle wasting marker genes including F-box only protein 32 (Atrogin-1) and muscle-specific RING-finger 1 (Murf-1) in diabetic group both in vitro and in vivo. Transformation from type I fibers (slow muscle) to type II fibers (fast muscle) were also observed under puerarin administration in diabetic rats. Puerarin promoted Akt/mTOR while inhibited LC3/p62 signaling pathway in skeletal muscle cells. In conclusion, our study showed that puerarin mitigated skeletal muscle wasting in T1D rats and closely related with Akt/mTOR activation and autophagy inhibition. Whether this effect in murine applies to humans remains to be determined.
- Published
- 2021
- Full Text
- View/download PDF
13. Teaching Evaluation Algorithm Based on Grey Relational Analysis
- Author
-
Xiaoying Zhang, Xiuying Yang, and Jing Yang
- Subjects
Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Grey correlation analysis uses grey correlations to describe the strength, magnitude, and order of relationships among factors. The data sequence of the drmined data is used as a reference data string, and the data sequence matrix of each influence factor is used as a control data matrix to calculate the correlation between the data sequence and the reference of each factor in the control data matrix. The basic idea of quantitatively measuring the correlation between each factor and the object is to determine the similarity between the reference data sequence and the shape of multiple comparison data sequences and to determine whether the connection is strong. It reflects the degree of correlation between curves. In this paper, we examine the problem of evaluating the quality of undergraduate education by multiple indicators and establish a comprehensive evaluation model of the quality of undergraduate education in the 13 prefecture level cities by using the grey correlation analysis. The correlation coefficient of each index was obtained by the grey correlation analysis, the correlation number of each index was analyzed and ranked, and finally the improvement of the number of “advanced teachers” and the education of 13 prefectures were reduced as much as possible.
- Published
- 2021
- Full Text
- View/download PDF
14. Experimental Study and Numerical Simulation on Mechanical Properties of the Bottom Plate in the Assembled Composite Slab with Additional Steel Trusses
- Author
-
Xiuying Yang, Yuzhuo Wang, Yongxin Liu, and Zhenyu Wei
- Subjects
Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The composite slab with steel trusses is composed of precast bottom plate and cast-in-place concrete. In engineering applications, cracks often appear in the bottom plate before casting the upper concrete, which even leads to the failure of the composite slab. To improve the crack resistance of the slab, a composite slab with additional steel trusses is proposed; that is, on the basis of the original longitudinal steel trusses, the transverse steel trusses are added. Static test and numerical analysis were carried out on the bottom plate of the new type of composite slab with the additional transverse steel trusses. The experimental and analytical results show that the load level of the plate with additional steel trusses can be increased by 33% under the normal service limit state; the deflection of the plate is significantly reduced and the crack development is effectively controlled, which illustrates that the new type of composite slab can improve the bearing capacity, increase the bending stiffness, and enhance the crack resistance effectively.
- Published
- 2021
- Full Text
- View/download PDF
15. Multivalent Aptamer Approach: Designs, Strategies, and Applications
- Author
-
Zhong Wang, Xiuying Yang, Nicholas Zhou Lee, and Xudong Cao
- Subjects
aptamers ,detection ,multivalence ,avidity ,antibodies ,biosensors ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
Aptamers are short and single-stranded DNA or RNA molecules with highly programmable structures that give them the ability to interact specifically with a large variety of targets, including proteins, cells, and small molecules. Multivalent aptamers refer to molecular constructs that combine two or more identical or different types of aptamers. Multivalency increases the avidity of aptamers, a particularly advantageous feature that allows for significantly increased binding affinities in comparison with aptamer monomers. Another advantage of multivalency is increased aptamer stabilities that confer improved performances under physiological conditions for various applications in clinical settings. The current study aims to review the most recent developments in multivalent aptamer research. The review will first discuss structures of multivalent aptamers. This is followed by detailed discussions on design strategies of multivalent aptamer approaches. Finally, recent developments of the multivalent aptamer approach in biosensing and biomedical applications are highlighted.
- Published
- 2022
- Full Text
- View/download PDF
16. Comprehensive Lipidome Profiling of the Kidney in Early-Stage Diabetic Nephropathy
- Author
-
Biyu Hou, Ping He, Peng Ma, Xinyu Yang, Chunyang Xu, Sin Man Lam, Guanghou Shui, Xiuying Yang, Li Zhang, Guifen Qiang, and Guanhua Du
- Subjects
diabetic nephropathy ,lipidomics ,glomerular filtration barrier ,lipotoxicity ,sphingolipids ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Metabolic changes associated with diabetes are reported to lead to the onset of early-stage diabetic nephropathy (DN). Furthermore, lipotoxicity is implicated in renal dysfunction. Most studies of DN have focused on a single or limited number of lipids, and the lipidome of the kidney during early-stage DN remains to be elucidated. In the present study, we aimed to comprehensively identify lipid abnormalities during early-stage DN; to this end, we established an early-stage DN rat model by feeding a high-sucrose and high-fat diet combined with administration of low-dose streptozotocin. Using a high-coverage, targeted lipidomic approach, we established the lipid profile, comprising 437 lipid species and 25 lipid classes, of the kidney cortex in normal rats and the DN rat model. Our findings additionally confirmed that the DN rat model had been successfully established. We observed distinct lipidomic signatures in the DN kidney, with characteristic alterations in side chain composition and degree of unsaturation. Glyceride lipids, especially cholesteryl esters, showed a significant increase in the DN kidney cortex. The levels of most phospholipids exhibited a decline, except those of phospholipids with side chain of 36:1. Furthermore, the levels of lyso-phospholipids and sphingolipids, including ceramide and its derivatives, were dramatically elevated in the present DN rat model. Our findings, which provide a comprehensive lipidome of the kidney cortex in rats with DN, are expected to be useful for the identification of pathologically relevant lipid species in DN. Furthermore, the results represent novel insights into the mechanistic basis of DN.
- Published
- 2020
- Full Text
- View/download PDF
17. Phylogenetic, comparative genomic and structural analyses of human Streptococcus agalactiae ST485 in China
- Author
-
Rui Wang, Liping Li, Ting Huang, Yan Huang, Weiyi Huang, Xiuying Yang, Aiying Lei, and Ming Chen
- Subjects
Group B Streptococcus ,CC103 ,ST485 ,Lac.2 ,CadDX ,Cross infection ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Streptococcus agalactiae (Group B Streptococcus, GBS) is a common bacteria species infecting both human and bovine. Previous studies have shown that the GBS isolated from human and bovine are mostly unrelated and belong to separate populations. However, recently, the bovine GBS CC103 has become the dominant epidemic strain and frequently isolated from human patients. In particular, the ST485 GBS, a member of CC103, has become the new dominant ST in China and exhibited very high pathogenicity. This phenomenon is not consistent with the established understanding about the relationship between bovine and human GBS, which needs to be re-investigated. Results The genome-based phylogenetic analysis showed that the human and bovine GBS CC103 strains had very close genetic relationship and they were alternately distributed on the evolutionary tree. CC103 strains evolved into several branches, including the ST485, which exhibited high pathogenicity and specifically infected human. Compared to other CC103 strains, the ST485 lacked Lac.2 gene structure and acquired the CadDX gene structure in their genomes. Conclusions Our results indicate that GBS CC103 could propagate across human and bovine, and GBS ST485 might evolve from the ST103 that could infect both human and bovine. Moreover, the recombination of Lac.2 and CadDX gene structures might play an important role in the formation of highly pathogenic ST485 in China.
- Published
- 2018
- Full Text
- View/download PDF
18. Salvianolic Acid A Protects Against Diabetic Nephropathy through Ameliorating Glomerular Endothelial Dysfunction via Inhibiting AGE-RAGE Signaling
- Author
-
Biyu Hou, Guifen Qiang, Yuerong Zhao, Xiuying Yang, Xi Chen, Yu Yan, Xiaobo Wang, Chenge Liu, Li Zhang, and Guanhua Du
- Subjects
Salvianolic acid A ,Advanced glycation end products ,Diabetic nephropathy ,Glomerular endothelia dysfunction ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Glomerular endothelium dysfunction leads to the progression of renal architectonic and functional abnormalities in early-stage diabetic nephropathy (DN). Advanced glycation end products (AGEs) and receptor for AGEs (RAGE) are proved to play important roles in diabetic nephropathy. This study investigated the role of Salvianolic acid A (SalA) on early-stage DN and its possible underlying mechanism. Methods: In vitro AGEs formation and breaking rate were measured to illustrate the effect of SalA on AGEs. Type 2 diabetic nephropathy rats were induced by high-fat diet and low-dose streptozocin (STZ). After eight-week treatment with SalA 1 mg/kg/day, 24h-urine protein, creatinine clearance was tested and renal structural injury was assessed by PAS and PASM staining. Primary glomerular endothelial cell permeability was evaluated after exposed to AGEs. AGEs-induced RhoA/ROCK and subsequently activated disarrange of cytoskeleton were assessed by western blot and immunofluorescence. Results: Biochemical assay and histological examination demonstrated that SalA markedly reduced endothelium loss and glomerular hyperfiltration, suppressed glomerular hypertrophy and mesangial matrix expansion, eventually reduced urinary albumin and ameliorated renal function. Further investigation suggested that SalA exerted its renoprotective effects through inhibiting AGE-RAGE signaling. It not only inhibited formation of AGEs and increased its breaking in vitro, but also reduced AGEs accumulation in vivo and downregulated RAGE expression. SalA restored glomerular endothelial permeability through suppressing AGEs-induced rearrangement of actin cytoskeleton via AGE-RAGE-RhoA/ ROCK pathway. Moreover, SalA attenuated oxidative stress induced by AGEs, subsequently alleviated inflammation and restored the disturbed autophagy in glomerular endothelial cell and diabetic rats via AGE-RAGE-Nox4 axis. Conclusion: Our study indicated that SalA restored glomerular endothelial function and alleviated renal structural deterioration through inhibiting AGE-RAGE, thus effectively ameliorated early-stage diabetic nephropathy. SalA might be a promising therapeutic agent for the treatment of diabetic nephropathy.
- Published
- 2017
- Full Text
- View/download PDF
19. Applying Active Learning Strategies to Develop the Professional Teaching Competency of Chinese College Student Teachers in the Context of Geography Education
- Author
-
Xiuying Yang and Pengfei Chen
- Subjects
Education - Abstract
Developing the professional teaching competence of college student teachers is a vital task in initial teacher education institutions. The aim of this research, based on a quasi-experimental design, was to apply active learning strategies to develop the professional teaching competence of Chinese college geography student teachers. In total, 96 geography student teachers in China participated in this research; 50 of them were placed in the experimental group, and the remaining 46 were placed in the control group. The intervention on active learning strategies was based on Bloom’s taxonomy and the Standards for The Professional Competence of Teachers majoring in Secondary Education for Student Teachers. The experimental group received the experimental intervention for 16 lessons, while the control group was exposed to the traditional teaching in the same teaching schedule. The results of the one-way ANCOVA undertaken show that the intervention consisting of active learning strategies significantly improved the professional competence of the Chinese college student geography teachers in the experimental group. This study contributes empirically to the universities, educators, and researchers involved in initial teacher education programmes in the geography field.
- Published
- 2022
20. Comparison between an SGLT2 inhibitor and insulin in tumor-to-tissue contrasts in 18F-FDG PET imaging of diabetic mice.
- Author
-
Meidai, Liang, Yujing, Du, Zhaoyu, Liu, Shanshi, Li, Guangyu, Zhao, Yan, Fan, Xiuying, Yang, and Jianhua, Zhang
- Subjects
SODIUM-glucose cotransporter 2 inhibitors ,SODIUM-glucose cotransporters ,POSITRON emission tomography ,INSULIN ,TUMOR diagnosis ,MICE ,SKELETAL muscle ,DAPAGLIFLOZIN - Abstract
18 F-fluorodeoxyglucose positron emission tomography (18 F-FDG PET) has been widely utilized for tumor diagnosis. Hyperglycemia affects the18 F-FDG uptake and reduces tumor-to-tissue contrasts, however, ideal hypoglycemic drugs are lacking. This study compared the role of insulin with the novel widely used hypoglycemic drug, sodium-glucose cotransporter 2 (SGLT2) inhibitor, on18 F-FDG PET imaging in diabetic conditions. The streptozotocin (STZ)-induced diabetic C57BL/6N mice were inoculated with B16 (mouse melanoma) cells to establish the xenograft tumor model. After the mice had been administrated with dapagliflozin (30 mg/kg, IG) or insulin (0.75 U/kg, IP) for one hour, 9.25 MBq/10 g18 F-FDG was injected. Biodistributions were detected by gamma counting and microPET imaging. The results showed dapagliflozin did not significantly affect the18 F-FDG uptake in tumors but reduced uptake in reference tissues, resulting in a significant increase in the tumor-to-skeletal muscle ratio. Conversely, insulin increased18 F-FDG uptake in tumors without significant reduction in uptake in reference tissues; Although there was an observable improvement in tumor imaging, it did not reach significantly statistical differences. This study suggests that insulin and SGLT2 inhibitor yield comparable effects on the quality of18 F-FDG PET imaging in diabetic patients. Nevertheless, SGLT2 inhibitors would be more suitable when skeletal muscle is used as reference tissue. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
21. The obesity-induced transcriptional regulator TRIP-Br2 mediates visceral fat endoplasmic reticulum stress-induced inflammation
- Author
-
Guifen Qiang, Hyerim Whang Kong, Difeng Fang, Maximilian McCann, Xiuying Yang, Guanhua Du, Matthias Blüher, Jinfang Zhu, and Chong Wee Liew
- Subjects
Science - Abstract
Visceral and subcutaneous fat are associated with different metabolic risk, but mediators of such depot specific effects are not very well known. Here the authors identify the transcriptional regulator, TRIP-Br2, as a regulator of endoplasmic reticulum (ER) stress-induced inflammatory responses specifically in visceral fat.
- Published
- 2016
- Full Text
- View/download PDF
22. High Incidence of Pathogenic Streptococcus agalactiae ST485 Strain in Pregnant/Puerperal Women and Isolation of Hyper-Virulent Human CC67 Strain
- Author
-
Liping Li, Rui Wang, Yan Huang, Ting Huang, Fuguang Luo, Weiyi Huang, Xiuying Yang, Aiying Lei, Ming Chen, and Xi Gan
- Subjects
group B streptococcus ,epidemiology ,clonal complex (CC) ,Tilapia ,ST485 ,Microbiology ,QR1-502 - Abstract
Group B streptococcus (GBS) is the major pathogen causing diseases in neonates, pregnant/puerperal women, cows and fish. Recent studies have shown that GBS may be infectious across hosts and some fish GBS strain might originate from human. The purpose of this study is to investigate the genetic relationship of CC103 strains that recently emerged in cows and humans, and explore the pathogenicity of clinical GBS isolates from human to tilapia. Ninety-two pathogenic GBS isolates were identified from 19 patients with different diseases and their evolution and pathogenicity to tilapia were analyzed. The multilocus sequence typing revealed that clonal complex (CC) 103 strain was isolated from 21.74% (20/92) of patients and ST485 strain was from 14.13% (13/92) patients with multiple diseases including neonates. Genomic evolution analysis showed that both bovine and human CC103 strains alternately form independent evolutionary branches. Three CC67 isolates carried gbs2018-C gene and formed one evolutionary branch with ST61 and ST67 strains that specifically infect dairy cows. Studies of interspecies transmission to tilapia found that 21/92 (22.83%) isolates including all ST23 isolates were highly pathogenic to tilapia and demonstrated that streptococci could break through the blood-brain barrier into brain tissue. In conclusions, CC103 strains are highly prevalent among pathogenic GBS from humans and have evolved into the highly pathogenic ST485 strains specifically infecting humans. The CC67 strains isolated from cows are able to infect humans through evolutionary events of acquiring CC17-specific type C gbs2018 gene and others. Human-derived ST23 pathogenic GBS strains are highly pathogenic to tilapia.
- Published
- 2018
- Full Text
- View/download PDF
23. Experimental Study and Numerical Simulation on Mechanical Properties of the Bottom Plate in the Assembled Composite Slab with Additional Steel Trusses
- Author
-
Zhenyu Wei, Yuzhuo Wang, Xiuying Yang, and Yongxin Liu
- Subjects
Materials science ,Article Subject ,business.industry ,Truss ,Structural engineering ,Engineering (General). Civil engineering (General) ,Deflection (engineering) ,Casting (metalworking) ,Precast concrete ,Bending stiffness ,Slab ,Limit state design ,Bearing capacity ,TA1-2040 ,business ,Civil and Structural Engineering - Abstract
The composite slab with steel trusses is composed of precast bottom plate and cast-in-place concrete. In engineering applications, cracks often appear in the bottom plate before casting the upper concrete, which even leads to the failure of the composite slab. To improve the crack resistance of the slab, a composite slab with additional steel trusses is proposed; that is, on the basis of the original longitudinal steel trusses, the transverse steel trusses are added. Static test and numerical analysis were carried out on the bottom plate of the new type of composite slab with the additional transverse steel trusses. The experimental and analytical results show that the load level of the plate with additional steel trusses can be increased by 33% under the normal service limit state; the deflection of the plate is significantly reduced and the crack development is effectively controlled, which illustrates that the new type of composite slab can improve the bearing capacity, increase the bending stiffness, and enhance the crack resistance effectively.
- Published
- 2021
24. Antidiabetic Effect of Salvianolic Acid A on Diabetic Animal Models via AMPK Activation and Mitochondrial Regulation
- Author
-
Guifen Qiang, Xiuying Yang, Lili Shi, Hengai Zhang, Bainian Chen, Yan Zhao, Mian Zu, Dan Zhou, Jing Guo, Haiguang Yang, Li Zhang, and Guanhua Du
- Subjects
Salvianolic acid A ,Diabetes mellitus ,Antidiabetic ,Mitochondria ,AMPK ,ATP ,Mitochondrial membrane potential ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Diabetes mellitus (DM) characterized by hyperglycemia contributes to macrovascular and microvascular complications. Salvianolic acid A (SalA) is a polyphenolic compound isolated from the root of Salvia miltiorrhiza Bunge, which is a traditional Chinese medicine widely used to treat cardiovascular diseases. However, little is known about its antidiabetic effect. Our study aimed to investigate the in vivo and in vitro antidiabetic effect of SalA and the underlying mechanisms. Methods: Alloxan-induced type 1 diabetic mice and high-fat diet (HFD) and low-dose streptozotocin (STZ)-induced type 2 diabetic rats received SalA treatment. Blood glucose, oral glucose tolerance test (OGTT), 24-h food and water intake were monitored. In vitro, glucose consumption and uptake were measured in HepG2 cells and L6 myotubes. Mitochondrial function was detected in hepatic and skeletal muscle mitochondria. AMP-activated protein kinase (AMPK) and Akt were analyzed by western blot. Results: In both type 1 and type 2 diabetic animals, SalA lowered fasting blood glucose (FBG) and fed blood glucose in dose-dependent manner, as well as reduced 24-h food and water intake. In vitro, SalA caused dose-dependent increase in glucose consumption and enhanced glucose uptake. SalA significantly increased ATP production from 10 min to 12 h in HepG2 cells and L6 myotubes. Interestingly, SalA decreased mitochondrial membrane potential (MMP) in HepG2 cells. Furthermore, SalA improved hepatic and skeletal muscle mitochondrial function, increased ATP production, and concurrently decreased MMP. In particularly, SalA activated AMPK phosphorylation through Ca2+/calmodulin-dependent protein kinase kinase β (CaMKKβ)/AMPK signaling pathway, independent of liver kinase 1 (LKB1)/AMPK pathway. However, SalA didn't show any effect on insulin secretagogue and activation of PI3K/Akt signaling pathway. Conclusion: SalA exhibits the antidiabetic effects in diabetic animal models through improving mitochondrial function, increasing ATP production, and decreasing MMP via CaMKKβ/AMPK signaling pathway.
- Published
- 2015
- Full Text
- View/download PDF
25. Urban trees in university campus: structure, function, and ecological values
- Author
-
Xiaohan Qu, Bing Huang, Junjiao Sun, Xiuying Yang, Zeming Li, Xueyan Wang, Xiaoqing Wei, and Yanlin Wang
- Subjects
Air Pollutants ,Universities ,biology ,Ecology ,Health, Toxicology and Mutagenesis ,Diameter at breast height ,Acer ,General Medicine ,010501 environmental sciences ,biology.organism_classification ,01 natural sciences ,Pollution ,Tree (graph theory) ,Trees ,Rainwater harvesting ,Ecosystem services ,Salix babylonica ,Geography ,Catalpa ovata ,Rhus typhina ,Humans ,Environmental Chemistry ,Interception ,Ecosystem ,0105 earth and related environmental sciences - Abstract
University campuses usually have more trees and can provide various ecosystem services. However, there are few reports on tree ecosystem services of Chinese university campuses, especially in northern China. This study investigated the trees in the campus of Shenyang Institute of Technology and analyzed its ecological benefits and monetary value through i-Tree Streets. The campus trees contained a total of 5193 trees of 66 species, of which Catalpa ovata G. Don, Acer mono Maxim., Rhus typhina Nutt, and Salix babylonica L. accounted for 59.7% of the total number. The age structure of the trees in the campus was not ideal, with 71.5% of young trees, 24.0% of maturing trees, 4.5% of mature trees, and only 0.04% of old trees. The trees in the campus provided more energy saving benefits ($60,850, $11.7/tree), carbon reduction benefits ($34,318, $6.6/tree) and aesthetic benefits ($30,150, $5.8/tree). The benefits resulted from air pollutant removal ($12,889, $2.5/tree) and rainwater runoff interception ($15,534, $3.0/tree) were smaller. In addition, tree species with more maturing trees and mature trees (i.e., with larger diameter at breast height) and large leaf area in the campus contributed significantly to ecosystem services. Our results can provide suggestions and certain insights for Chinese campus greening managers in tree species selection and tree management.
- Published
- 2021
26. Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
- Author
-
Ping He, Biyu Hou, Yanliang Li, Chunyang Xu, Peng Ma, Sin Man Lam, Victoria Gil, Xinyu Yang, Xiuying Yang, Li Zhang, Guanghou Shui, Junke Song, Guifen Qiang, Chong Wee Liew, and Guanhua Du
- Subjects
lipid profiling ,white adipose tissue ,browning heterogeneity ,visceral adipose tissue ,subcutaneous adipose tissue ,Microbiology ,QR1-502 - Abstract
Background: White adipose tissue (WAT) browning confers beneficial effects on metabolic diseases. However, visceral adipose tissue (VAT) is not as susceptible to browning as subcutaneous adipose tissue (SAT). Aim: Interpreting the heterogeneity of VAT and SAT in brown remodeling and provide promising lipid targets to promote WAT browning. Methods: We first investigated the effects of β3-adrenergic stimulation by CL316,243 on systemic metabolism. Then, high-coverage targeted lipidomics approach with multiple reaction monitoring (MRM) was utilized to provide extensive detection of lipid metabolites in VAT and SAT. Results: CL316,243 notably ameliorated the systemic metabolism and induced brown remodeling of SAT but browning resistance of VAT. Comprehensive lipidomics analysis revealed browning heterogeneity of VAT and SAT with more dramatic alteration of lipid classes and species in VAT rather than SAT, though VAT is resistant to browning. Adrenergic stimulation differentially affected glycerides content in VAT and SAT and boosted the abundance of more glycerophospholipids species in VAT than in SAT. Besides, CL316,243 increased sphingolipids in VAT without changes in SAT, meanwhile, elevated cardiolipin species more prominently in VAT than in SAT. Conclusions: We demonstrated the browning heterogeneity of WAT and identified potential lipid biomarkers which may provide lipid targets for overcoming VAT browning resistance.
- Published
- 2019
- Full Text
- View/download PDF
27. Ratiometric fluorescence probe of Cu2+ and biothiols by using carbon dots and copper nanoclusters
- Author
-
Zheng Huang, Xiuying Yang, Longshan Zhao, Jianqiang Song, Ning Zhao, and Yousheng Wang
- Subjects
inorganic chemicals ,Detection limit ,Absorption (pharmacology) ,General Chemical Engineering ,General Chemistry ,Glutathione ,Photochemistry ,Fluorescence ,Photoinduced electron transfer ,chemistry.chemical_compound ,Förster resonance energy transfer ,chemistry ,Chelation ,Cysteine - Abstract
A novel dual-emission ratiometric fluorescent probe based on N-doped yellow fluorescent carbon dots (y-CDs) and blue fluorescent copper nanoclusters (CuNCs) was established for quantitative determination of Cu2+ and biothiols. In this work, the Cu2+-(y-CDs) complexes formed by the chelation of y-CDs with Cu2+, showed an absorption peak at 430 nm that not only enhanced the fluorescence of y-CDs through inhibiting photoinduced electron transfer (PET) but also effectively quenched the fluorescence of CuNCs due to Forster resonance energy transfer (FRET). In addition, the chelation of y-CDs with Cu2+ could be inhibited by biothiols that prevented the fluorescence of y-CDs from being enhanced and the fluorescence of CuNCs from being quenched. On account of the changes of ratiometric signal, a dual-emission fluorescence probe for Cu2+ and biothiols determination was achieved. The proposed method exhibited high sensitivity for Cu2+ and biothiols in the ranges of 0.5–100 μM and 0.8–50 μM and the limits of detection (LODs) of Cu2+, glutathione (GSH), cysteine (Cys) and homocysteine (Hcy) were 0.21 μM, 0.33 μM, 0.39 μM and 0.46 μM, respectively. Subsequently, the established strategy presented an application prospect for the detection of Cu2+ and biothiols in real samples.
- Published
- 2021
28. EETs mediate cardioprotection of salvianolic acids through MAPK signaling pathway
- Author
-
Shoubao Wang, Weiku Zhang, Xiaobin Pang, Li Li, Guorong He, Xiuying Yang, Lianhua Fang, Juntian Zhang, and Guanhua Du
- Subjects
Salvianolic acids ,Acute myocardial infarction ,EETs/sEH ,MAPK ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Salvianolic acids, including salvianolic acid A (SAA) and salvianolic acid B (SAB), are the main water-soluble bioactive compounds isolated from the Chinese medicinal herb Danshen and have been shown to exert in vitro and in vivo cardiovascular protection. Recent studies suggest that epoxyeicosatrienoic acids (EETs), the primary cytochrome P450 2J (CYP2J) epoxygenase metabolites of arachidonic acid, are involved in the progression of ischemic injury in diverse organs. Here, we investigated the relation between the protective effects of salvianolic acids and EETs/sEH as well as MAPK signaling pathway. In the present study, the rat acute myocardial infarction (AMI) model was established by the left anterior descending coronary artery occlusion. Our results showed that salvianolic acids significantly reduced ST-segment elevation and serum levels of CK-MB, LDH, and ALT in AMI rats, and significantly attenuated the caspase 3 expression and reduced the ratio of Bax/Bcl-2. ELISA measurement showed that salvianolic acids significantly increased the 14,15-EET levels in blood and heart, and attenuated hydrolase activity of sEH in heart of AMI rat. Western blotting analysis suggested that salvianolic acids significantly attenuated the phosphorylation of JNK and p38, and increased phosphorylation of ERK in heart. In conclusion, these results indicate that EETs/sEH and MAPK signaling pathways are important processes in cardioprotection of salvianolic acids.
- Published
- 2013
- Full Text
- View/download PDF
29. Enhanced Thermal Performance and Impact Strength of UHMWPE/Recycled-PA6 Blends Synthesized via a Melting Extrusion Route
- Author
-
Xiuying Yang, Junye Cheng, Guangping Zheng, and Deqing Zhang
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
The blends of ultra-high molecular weight polyethylene (UHMWPE) and recycled-polyamide 6 (R-PA6) were prepared via a melting extrusion route using high-density polyethylene-graft-maleic anhydride (HDPE-g-MAH) as the compatibilizer. The morphologies and distributions of the chemical components of the blends were characterized by scanning electron microscopy and synchrotron Fourier transform infrared microspectroscopy. The effects of R-PA6 content on the Vicat softening temperature (VST), heat distortion temperature (HDT), and impact strength of the blends were studied. Remarkably, in comparison with those of UHMWPE, the VST and HDT of UHMWPE/R-PA6 blends with 44 wt% R-PA6 were increased to 165.1 and 98.4°C, respectively, and the Charpy impact strength and Izod impact strength of the blends were enhanced to 33.9 and 16.2 kJ/m2, respectively. In addition, it was found that the blending system containing 44 wt% R-PA6 and 48 wt% UHMWPE exhibited the best compatibility when it was prepared using 8 wt% HDPE-g-MAH. The distribution of the phases of UHMWPE and R-PA6 was uniform, and no obvious phase separation was observed in the blends.
- Published
- 2016
- Full Text
- View/download PDF
30. From 'S' to 'O': experimental and theoretical insights into the atmospheric degradation mechanism of dithiophosphinic acids
- Author
-
Zhipeng Wang, Xueyu Wang, Songdong Ding, Jingjing Liu, Jun Li, Yixiang Zhang, Chao Xu, Lianjun Song, and Xiuying Yang
- Subjects
Lanthanide ,Ligand ,General Chemical Engineering ,chemistry.chemical_element ,General Chemistry ,Nickel ,chemistry.chemical_compound ,chemistry ,Computational chemistry ,Molecular orbital ,Hydroxyl radical ,Chemical stability ,Titration ,Selectivity - Abstract
Dithiophosphinic acids (DPAHs, expressed as R1R2PSSH) are a type of sulfur-donor ligand that have been vastly applied in hydrometallurgy. In particular, DPAHs have shown great potential in highly efficient trivalent actinide/lanthanide separation, which is one of the most challenging tasks in separation science and is of great importance for the development of an advanced fuel cycle in nuclear industry. However, DPAHs have been found liable to undergo oxidative degradation in the air, leading to significant reduction in the selectivity of actinide/lanthanide separation. In this work, the atmospheric degradation of five representative DPAH ligands was investigated for the first time over a sufficiently long period (180 days). The oxidative degradation process of DPAHs elucidated by ESI-MS, 31P NMR, and FT-IR analyses is R1R2PSSH → R1R2PSOH → R1R2POOH → R1R2POO–OOPR1R2, R1R2PSSH → R1R2PSS–SSPR1R2, and R1R2PSSH → R1R2PSOH → R1R2POS–SOPR1R2. Meanwhile, the determination of pKa values through pH titration and oxidation product by PXRD further confirms the S → O transformation in the process of DPAH deterioration. DFT calculations suggest that the hydroxyl radical plays the dominant role in the oxidation process of DPAHs and the order in which the oxidation products formed is closely related to the reaction energy barrier. Moreover, nickel salts of DPAHs have shown much higher chemical stability than DPAHs, which was also elaborated through molecular orbital (MO) and adaptive natural density portioning (AdNDP) analyses. This work unambiguously reveals the atmospheric degradation mechanism of DPAHs through both experimental and theoretical approaches. At the application level, the results not only provide an effective way to preserve DPAHs but could also guide the design of more stable sulfur-donor ligands in the future.
- Published
- 2020
31. Evaluation of the Development Potential of Hainan Free Trade Port Industrial Chain Driven by the Internet of Things Distribution Mechanism
- Author
-
Jing Yang, Xiaoying Zhang, and Xiuying Yang
- Subjects
China ,General Computer Science ,Article Subject ,General Mathematics ,General Neuroscience ,Internet of Things ,Industry ,Economic Development ,General Medicine ,Cities - Abstract
This paper starts from the industrial structure and characteristics of the Internet of Things and compares and analyzes the development ideas of the Internet of Things industry in the market economy under different systems. We also discuss IoT business model innovations and analyze the inadequacies of current IoT business models. We use the cost-benefit analysis method to analyze, from the aspects of social benefits, economic benefits, environmental benefits, and externalities. In this paper, factor analysis and quantitative statistics are used to calculate the weight of influencing factors, and the multilevel fuzzy evaluation method is used to score the influencing factor indicators to calculate the final score result, so as to judge the applicability of the multilevel fuzzy evaluation method to judge the profit model. In order to verify whether the establishment of economic regions plays a positive role in regional economic development, a double-difference model is established. Two sets of data from the Free Trade Zone and Hainan Free Trade Zone are selected for regression analysis, and robustness tests of various methods are carried out. This paper regresses various samples of the Hainan Free Trade Zone by region and city level to explore their characteristics in different regions. From the empirical results, it can be seen that the establishment of the Hainan Free Trade Zone has indeed promoted the economic development of the region where it is located, and this result is still significant after repeated robustness tests. Judging from the regression results by region, the establishment of the Hainan Free Trade Zone has a greater role in promoting the economic development of the eastern and western regions and it has a stronger pulling effect on the western regions than in the western regions. The policy effect of establishing a free trade zone may not be significant due to too few samples and short establishment time.
- Published
- 2022
- Full Text
- View/download PDF
32. Nursing Effect and Prognosis Analysis of Self-Management Education Model Based on Protective Motivation Theory on Patients with Hematological Tumor after PICC Catheterization
- Author
-
Lili Jiang, Xiuying Yang, Lu Xi, and Chaolang Zhang
- Subjects
Catheterization, Central Venous ,Motivation ,General Immunology and Microbiology ,Applied Mathematics ,Modeling and Simulation ,Hematologic Neoplasms ,Self-Management ,Humans ,General Medicine ,Prognosis ,General Biochemistry, Genetics and Molecular Biology - Abstract
Objective. To analyze the nursing effect and prognostic value of self-management education model based on protective motivation theory on patients with hematological malignancies (HM) after peripheral venous catheterization (PICC). Methods. A total of 90 patients with HMs treated with PICC catheterization in our hospital from October 2019 to October 2021 were included in the study. The patients were randomly assigned into experimental group ( n = 45 ) and control group ( n = 45 ). On the basis of the control group, the experimental group implemented the self-management education model based on the theory of protective motivation, and the control group implemented routine nursing health education measures. The self-nursing ability scale (ESCA), Hamilton anxiety scale (HAMA), the average cost of PICC catheter maintenance, the incidence of PICC-related complications, and the score of Newcastle nursing satisfaction scale (NSNS) were observed 1 month before and after nursing. Results. After one month of nursing, there was no significant difference in ESCA self-concept ( p > 0.05 ). After one month of nursing, the scores of self-care responsibility, self-nursing skills, and health knowledge in the experimental group were significantly higher compared to those before nursing ( p < 0.05 ) and higher compared to those in the control group ( p < 0.05 ). After one month of nursing, the score of HAMA scale was significantly lower compared to that before nursing ( p < 0.05 ), and the score of HAMA scale in the experimental group was lower compared to the control group ( p < 0.05 ). After one month of nursing, the average cost of PICC pipeline maintenance in the test group was lower than that in the control group ( p < 0.05 ). One month after nursing, the incidence of PICC-related complications in the experimental group was lower than that in the control group ( p < 0.05 ). One month after nursing, the score of NSNS scale was significantly higher than that before nursing ( p < 0.05 ). After one month of nursing, the score of NSNS scale in the experimental group was higher than that in the control group. Conclusion. The self-management education model based on protective motivation theory is effective in nursing patients with peripheral venous catheterization and PICC catheterization of HMs. It is more beneficial to promote patients’ self-nursing management ability, reduce anxiety, reduce the average cost of PICC pipeline maintenance, reduce the incidence of PICC-related complications, and enhance nursing satisfaction.
- Published
- 2022
- Full Text
- View/download PDF
33. Impact of Al nanoparticles upon the microstructure and wear properties of Ni-Cr-Al nanocomposite coatings
- Author
-
Xiuying Yang, Yuebo Zhou, Mingyu Zhao, Pai Liu, Hualong Qi, and Yufang Yang
- Subjects
Inorganic Chemistry ,Organic Chemistry ,Drug Discovery ,Electrochemistry ,Physical and Theoretical Chemistry - Published
- 2023
34. Salvianolic Acid A Protects the Peripheral Nerve Function in Diabetic Rats through Regulation of the AMPK-PGC1α-Sirt3 Axis
- Author
-
Guanhua Du, Lili Shi, Hengai Zhang, Zhonglin Huang, Huakang Huang, Li Zhang, Xiuying Yang, and Xiaoyan Yu
- Subjects
salvianolic acid A ,diabetic neuropathy ,AMP-activated protein kinase ,Organic chemistry ,QD241-441 - Abstract
Salvianolic acid A (SalA) is one of the main efficacious, water-soluble constituents of Salvia miltiorrhiza Bunge. This study investigated the protective effects of SalA on peripheral nerve in diabetic rats. Administration of SalA (0.3, 1 and 3 mg/kg, ig) was started from the 5th week after strepotozotocin (STZ60 mg/kg) intraperitoneal injection and continued for 8 weeks. Paw withdrawal mechanical threshold (PWMT) and motor nerve conduction velocity (MNCV) were used to assess peripheral nerve function. The western blot methods were employed to test the expression levels of serine-threonine liver kinase B1 (LKB1), AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1α), silent information regulator protein3 (sirtuin 3/Sirt3) and neuronal nitric oxide synthase (nNOS) in sciatic nerve. Results showed that SalA administration could increase PWMT and MNCV in diabetic rats; reduce the deterioration of sciatic nerve pathology; increase AMPK phosphorylation level, up-regulate PGC-1α, Sirt3 and nNOS expression, but had no influence on LKB1. These results suggest that SalA has protective effects against diabetic neuropathy. The beneficial effects of SalA on peripheral nerve function in diabetic rats might be attributed to improvements in glucose metabolism through regulation of the AMPK-PGC1α-Sirt3 axis.
- Published
- 2012
- Full Text
- View/download PDF
35. Muscle-generated BDNF (brain derived neurotrophic factor) maintains mitochondrial quality control in female mice
- Author
-
Chi Wai Lee, Suchaorn Saengnipanthkul, Chun Fai Ng, Wing Suen Chan, Xiuying Yang, Margaret Chui Ling Tse, Keqiang Ye, Jason K. Kim, Hye Lim Noh, Baowei Jiao, Daniel Brobst, Hiu-Lam Rachel Kwan, Oana Herlea-Pana, Brian Pak Shing Pang, Chi Bun Chan, Xinyi Bi, Palak Ahuja, and Guanhua Du
- Subjects
Brain-derived neurotrophic factor ,Mitochondrial fission factor ,Brain-Derived Neurotrophic Factor ,Fatty Acids ,PINK1 ,Cell Biology ,Tropomyosin receptor kinase B ,Biology ,AMP-Activated Protein Kinases ,Cell biology ,Mitochondria, Muscle ,DNM1L ,Mice ,Autophagy ,Animals ,Mitochondrial fission ,Female ,Protein kinase A ,Muscle, Skeletal ,Molecular Biology ,CAMKK2 ,Research Paper - Abstract
Mitochondrial remodeling is dysregulated in metabolic diseases but the underlying mechanism is not fully understood. We report here that BDNF (brain derived neurotrophic factor) provokes mitochondrial fission and clearance in skeletal muscle via the PRKAA/AMPK-PINK1-PRKN/Parkin and PRKAA-DNM1L/DRP1-MFF pathways. Depleting Bdnf expression in myotubes reduced fatty acid-induced mitofission and mitophagy, which was associated with mitochondrial elongation and impaired lipid handling. Muscle-specific bdnf knockout (MBKO) mice displayed defective mitofission and mitophagy, and accumulation of dysfunctional mitochondria in the muscle when they were fed with a high-fat diet (HFD). These animals also have exacerbated body weight gain, increased intramyocellular lipid deposition, reduced energy expenditure, poor metabolic flexibility, and more insulin resistance. In contrast, consuming a BDNF mimetic (7,8-dihydroxyflavone) increased mitochondrial content, and enhanced mitofission and mitophagy in the skeletal muscles. Hence, BDNF is an essential myokine to maintain mitochondrial quality and function, and its repression in obesity might contribute to impaired metabolism. Abbreviations: 7,8-DHF: 7,8-dihydroxyflavone; ACACA/ACC: acetyl Coenzyme A carboxylase alpha; ACAD: acyl-Coenzyme A dehydrogenase family; ACADVL: acyl-Coenzyme A dehydrogenase, very long chain; ACOT: acyl-CoA thioesterase; CAMKK2: calcium/calmodulin-dependent protein kinase kinase 2, beta; BDNF: brain derived neurotrophic factor; BNIP3: BCL2/adenovirus E1B interacting protein 3; BNIP3L/NIX: BCL2/adenovirus E1B interacting protein 3-like; CCL2/MCP-1: chemokine (C-C motif) ligand 2; CCL5: chemokine (C-C motif) ligand 5; CNS: central nervous system; CPT1B: carnitine palmitoyltransferase 1b, muscle; Cpt2: carnitine palmitoyltransferase 2; CREB: cAMP responsive element binding protein; DNM1L/DRP1: dynamin 1-like; E2: estrogen; EHHADH: enoyl-CoenzymeA hydratase/3-hydroxyacyl CoenzymeA dehydrogenase; ESR1/ER-alpha: estrogen receptor 1 (alpha); FA: fatty acid; FAO: fatty acid oxidation; FCCP: carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone; FFA: free fatty acids; FGF21: fibroblast growth factor 21; FUNDC1: FUN14 domain containing 1; HADHA: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha; HFD: high-fat diet; iWAT: inguinal white adipose tissues; MAP1LC3A/LC3A: microtubule-associated protein 1 light chain 3 alpha; MBKO; muscle-specific bdnf knockout; IL6/IL-6: interleukin 6; MCEE: methylmalonyl CoA epimerase; MFF: mitochondrial fission factor; NTRK2/TRKB: neurotrophic tyrosine kinase, receptor, type 2; OPTN: optineurin; PA: palmitic acid; PARL: presenilin associated, rhomboid-like; PDH: pyruvate dehydrogenase; PINK1: PTEN induced putative kinase 1; PPARGC1A/PGC-1α: peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; PRKAA/AMPK: protein kinase, AMP-activated, alpha 2 catalytic subunit; ROS: reactive oxygen species; TBK1: TANK-binding kinase 1; TG: triacylglycerides; TNF/TNFα: tumor necrosis factor; TOMM20: translocase of outer mitochondrial membrane 20; ULK1: unc-51 like kinase 1
- Published
- 2021
36. Ratiometric fluorescence probe of Cu
- Author
-
Ning, Zhao, Jianqiang, Song, Zheng, Huang, Xiuying, Yang, Yousheng, Wang, and Longshan, Zhao
- Abstract
A novel dual-emission ratiometric fluorescent probe based on N-doped yellow fluorescent carbon dots (y-CDs) and blue fluorescent copper nanoclusters (CuNCs) was established for quantitative determination of Cu
- Published
- 2021
37. Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung
- Author
-
Can Zhou, Guifen Qiang, Bowen Li, He Tian, Peng Ma, Xiuying Yang, Zijing Wang, Guanghou Shui, Yisa Wang, Guanhua Du, Biyu Hou, and Chengqian Yin
- Subjects
Male ,0301 basic medicine ,Programmed cell death ,Science ,Sulfuric Acid Esters ,Biochemistry ,Article ,Transcriptome ,Cresols ,Methylamines ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Metabolomics ,Animals ,Lung ,Gene ,Multidisciplinary ,Chemistry ,Gene Expression Profiling ,Endoplasmic reticulum ,RNA ,Lung Injury ,Endoplasmic Reticulum Stress ,Glutathione ,Computational biology and bioinformatics ,Cell biology ,Mice, Inbred C57BL ,Oxidative Stress ,030104 developmental biology ,030220 oncology & carcinogenesis ,Unfolded Protein Response ,Unfolded protein response ,Medicine ,Signal transduction ,Biomarkers - Abstract
Endoplasmic reticulum (ER) stress is a cellular state that results from the overload of unfolded/misfolded protein in the ER that, if not resolved properly, can lead to cell death. Both acute lung infections and chronic lung diseases have been found related to ER stress. Yet no study has been presented integrating metabolomic and transcriptomic data from total lung in interpreting the pathogenic state of ER stress. Total mouse lungs were used to perform LC–MS and RNA sequencing in relevance to ER stress. Untargeted metabolomics revealed 16 metabolites of aberrant levels with statistical significance while transcriptomics revealed 1593 genes abnormally expressed. Enrichment results demonstrated the injury ER stress inflicted upon lung through the alteration of multiple critical pathways involving energy expenditure, signal transduction, and redox homeostasis. Ultimately, we have presented p-cresol sulfate (PCS) and trimethylamine N-oxide (TMAO) as two potential ER stress biomarkers. Glutathione metabolism stood out in both omics as a notably altered pathway that believed to take important roles in maintaining the redox homeostasis in the cells critical for the development and relief of ER stress, in consistence with the existing reports.
- Published
- 2021
38. Controllable synthesis and characterization of tungsten disulfide nanosheets as promising nanomaterials for electronic devices
- Author
-
Shuang Liang, Junye Cheng, Tingting Liu, Guangping Zheng, Deqing Zhang, Jixing Chai, Mao-Sheng Cao, Xiuying Yang, and Hao Wang
- Subjects
Materials science ,Process Chemistry and Technology ,Tungsten disulfide ,chemistry.chemical_element ,Tungsten ,equipment and supplies ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Nanomaterials ,chemistry.chemical_compound ,chemistry ,X-ray photoelectron spectroscopy ,Chemical engineering ,Materials Chemistry ,Ceramics and Composites ,Tungsten hexachloride ,High-resolution transmission electron microscopy ,Sodium diethyldithiocarbamate ,Nanosheet - Abstract
The hydrothermal method was utilized to synthesize flat sheet-like tungsten disulfide with a single-crystal structure, using sodium diethyldithiocarbamate, tungsten hexachloride and cetyltrimethylammonium bromide as a reducing agent and a sulfur source, a tungsten source and a surfactant, respectively. The controllable synthesis of the tungsten disulfide nanosheet was realized by controlling the types of surfactants, which was characterized by XRD, FTIR, XPS, SEM, TEM, HRTEM, BET, UV-vis and PL. Due to the unique flat sheet morphology and unique electronic characteristic of the synthesized tungsten disulfide nanosheets, they could be promising nanomaterials for electronic devices.
- Published
- 2019
39. Catalyst-Free Synthesis of Hollow-Sphere-Like ZnO and Its Photoluminescence Property
- Author
-
Junye Cheng, Xiuying Yang, Hua Tian, Bin Zhao, and Deqing Zhang
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Hollow-sphere-like ZnO was successfully prepared by a facile combustion route at 950°C, and no external catalysts or additives were introduced. The morphology and structure of the hollow-sphere-like ZnO were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), and energy dispersive spectrometer (EDS). The possible growth mechanism was discussed in detail. In addition, the as-obtained hollow-sphere-like ZnO exhibited a strong green emission at 518 nm and a weak UV emission at 385 nm. We believe that the hollow-sphere-like ZnO material may be a good candidate for application in optical devices and catalyst systems.
- Published
- 2014
- Full Text
- View/download PDF
40. Comprehensive Lipidome Profiling of the Kidney in Early-Stage Diabetic Nephropathy
- Author
-
Ping He, Guanghou Shui, Li Zhang, Xiuying Yang, Xinyu Yang, Sin Man Lam, Guanhua Du, Biyu Hou, Peng Ma, Guifen Qiang, and Chunyang Xu
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Ceramide ,Kidney Cortex ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Rats, Sprague-Dawley ,Diabetic nephropathy ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,Lipidomics ,medicine ,Animals ,Diabetic Nephropathies ,Kidney ,sphingolipids ,lcsh:RC648-665 ,medicine.diagnostic_test ,diabetic nephropathy ,Lipidome ,lipotoxicity ,Lipid Metabolism ,medicine.disease ,Sphingolipid ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Lipotoxicity ,glomerular filtration barrier ,lipidomics ,lipids (amino acids, peptides, and proteins) ,Lipid profile - Abstract
Metabolic changes associated with diabetes are reported to lead to the onset of early-stage diabetic nephropathy (DN). Furthermore, lipotoxicity is implicated in renal dysfunction. Most studies of DN have focused on a single or limited number of lipids, and the lipidome of the kidney during early-stage DN remains to be elucidated. In the present study, we aimed to comprehensively identify lipid abnormalities during early-stage DN; to this end, we established an early-stage DN rat model by feeding a high-sucrose and high-fat diet combined with administration of low-dose streptozotocin. Using a high-coverage, targeted lipidomic approach, we established the lipid profile, comprising 437 lipid species and 25 lipid classes, of the kidney cortex in normal rats and the DN rat model. Our findings additionally confirmed that the DN rat model had been successfully established. We observed distinct lipidomic signatures in the DN kidney, with characteristic alterations in side chain composition and degree of unsaturation. Glyceride lipids, especially cholesteryl esters, showed a significant increase in the DN kidney cortex. The levels of most phospholipids exhibited a decline, except those of phospholipids with side chain of 36:1. Furthermore, the levels of lyso-phospholipids and sphingolipids, including ceramide and its derivatives, were dramatically elevated in the present DN rat model. Our findings, which provide a comprehensive lipidome of the kidney cortex in rats with DN, are expected to be useful for the identification of pathologically relevant lipid species in DN. Furthermore, the results represent novel insights into the mechanistic basis of DN.
- Published
- 2020
41. Preparation and mechanism of hydroxyapatite hollow microspheres with different surface charge by biomimetic method
- Author
-
Shangyou Xiao, Kebing Guo, Xiuying Yang, Zhenzhen Tian, Shilei Hao, Jingou Ji, and Ting Lu
- Subjects
Materials science ,Surface Properties ,Scanning electron microscope ,0206 medical engineering ,Biomedical Engineering ,Biophysics ,Nanoparticle ,Bioengineering ,02 engineering and technology ,Polyethylene glycol ,Polyethylene Glycols ,Biomaterials ,chemistry.chemical_compound ,Drug Delivery Systems ,X-Ray Diffraction ,Biomimetics ,Specific surface area ,PEG ratio ,Surface charge ,High-resolution transmission electron microscopy ,technology, industry, and agriculture ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Microspheres ,Durapatite ,chemistry ,Chemical engineering ,Microscopy, Electron, Scanning ,Nanoparticles ,0210 nano-technology ,Powder diffraction - Abstract
To meet the different application requirements in various fields, hydroxyapatite (HA) hollow microspheres with different surface charge were synthesized successfully by biomimetic method using Ca(NO3)2·4H2O and (NH4)2HPO4 in the presence of polyethylene glycol (PEG). Scanning electron microscopy (SEM), High-resolution TEM (HRTEM), X-ray powder diffraction (XRD), and Zeta PALS were used to characterize the obtained samples. The results indicated that the concentration of PEG and temperature significantly affect the morphology of the obtained samples. After incubation for 5 d, the HA hollow microspheres with positive surface charge, HA spherical nanoparticles with surface charge close to zero and calcium deficiency HA (d-HA) hollow microspheres with negative surface charge were obtained respectively in the presence of 5% PEG, 6% PEG and 7% PEG at 15 °C. Brunauer-Emmett-Teller (BET) revealed that the specific surface area of HA hollow microspheres reached 98.50 m2/g, while that of HA spherical nanoparticles were only 4.12 m2/g, hollow microspheres show a better application prospect. The possible formation mechanism was also discussed.
- Published
- 2020
42. Effects of fasting on the expression pattern of FGFs in different skeletal muscle fibre types and sexes in mice
- Author
-
Jin-Hua Wang, Nuoqi Wang, Xiuying Yang, Biyu Hou, Guanhua Du, Guifen Qiang, Wei-hua Jia, Lin Yin, Chi Bun Chan, and Xi Chen
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,FGF21 ,Muscle Fibers, Skeletal ,Fibroblast growth factor ,Skeletal muscle ,lcsh:Medicine ,Biology ,lcsh:Physiology ,Gender Studies ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,FGF8 ,Expression pattern ,In vivo ,Internal medicine ,medicine ,Animals ,Soleus muscle ,Sex Characteristics ,lcsh:QP1-981 ,Research ,lcsh:R ,Computational Biology ,Fasting ,FGF18 ,Fibroblast Growth Factors ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Sex ,Fibre type - Abstract
Fibroblast growth factors (FGFs) belong to a large family comprising 22 FGF polypeptides that are widely expressed in tissues. Most of the FGFs can be secreted and involved in the regulation of skeletal muscle function and structure. However, the role of fasting on FGF expression pattern in skeletal muscles remains unknown. In this study, we combined bioinformatics analysis and in vivo studies to explore the effect of 24-h fasting on the expression of Fgfs in slow-twitch soleus and fast-twitch tibialis anterior (TA) muscle from male and female C57BL/6 mice. We found that fasting significantly affected the expression of many Fgfs in mouse skeletal muscle. Furthermore, skeletal muscle fibre type and sex also influenced Fgf expression and response to fasting. We observed that in both male and female mice fasting reduced Fgf6 and Fgf11 in the TA muscle rather than the soleus. Moreover, fasting reduced Fgf8 expression in the soleus and TA muscles in female mice rather than in male mice. Fasting also increased Fgf21 expression in female soleus muscle and female and male plasma. Fasting reduced Fgf2 and Fgf18 expression levels without fibre-type and sex-dependent effects in mice. We further found that fasting decreased the expression of an FGF activation marker gene—Flrt2 in the TA muscle but not in the soleus muscle in both male and female mice. This study revealed the expression profile of Fgfs in different skeletal muscle fibre types and different sexes and provides clues to the interaction between the skeletal muscle and other organs, which deserves future investigations.
- Published
- 2020
43. Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2
- Author
-
Ping He, Wenlan Li, Biyu Hou, Guifen Qiang, Peng Ma, Chunyang Xu, Guanhua Du, Xiuying Yang, Xinyu Yang, and Li Zhang
- Subjects
Mitochondrial ROS ,Aging ,Article Subject ,NF-E2-Related Factor 2 ,Oxidative phosphorylation ,Pharmacology ,medicine.disease_cause ,Biochemistry ,Neuroprotection ,Cell Line ,Caffeic Acids ,Diabetic Neuropathies ,medicine ,Animals ,Viability assay ,chemistry.chemical_classification ,Inflammation ,Reactive oxygen species ,Gene knockdown ,QH573-671 ,Peripheral Nervous System Diseases ,Cell Biology ,General Medicine ,KEAP1 ,Sciatic Nerve ,Mitochondria ,Rats ,Mice, Inbred C57BL ,Oxidative Stress ,Glucose ,Neuroprotective Agents ,chemistry ,Lactates ,Cytology ,Oxidative stress ,Research Article - Abstract
Oxidative stress has been recognized as the contributor to diabetic peripheral neuropathy (DPN). Antioxidant strategies have been most widely explored; nevertheless, whether antioxidants alone prevent DPN still remains inconclusive. In the present study, we established an in vitro DPN cell model for drug screening using Schwann RSC96 cells under high glucose (HG) stimulation, and we found that salvianolic acid A (SalA) mitigated HG-induced injury evidenced by cell viability and myelination. Mechanistically, SalA exhibited strong antioxidative effects by inhibiting 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing reactive oxygen species (ROS), malondialdehyde (MDA), and oxidized glutathione (GSSG) content, as well as upregulating antioxidative enzyme mRNA expression. In addition, SalA significantly extenuated neuroinflammation with downregulated inflammatory factor mRNA expression. Furthermore, SalA improved the mitochondrial function of HG-injured Schwann cells by scavenging mitochondrial ROS, decreasing mitochondrial membrane potential (MMP), and enhancing ATP production, as well as upregulating oxidative phosphorylation gene expression. More importantly, we identified nuclear factor-E2-related factor 2 (Nrf2) as the upstream regulator which mediated protective effects of SalA on DPN. SalA directly bound to the Kelch domain of Kelch-like ECH-associated protein 1 (Keap1) and thus disrupted the interaction of Nrf2 and Keap1 predicted by LibDock of Discovery Studio. Additionally, SalA significantly inhibited Nrf2 promoter activity and downregulated Nrf2 mRNA expression but without affecting Nrf2 protein expression. Interestingly, SalA upregulated the nuclear Nrf2 expression and promoted Nrf2 nuclear translocation by high content screening assay, which was confirmed to be involved in its antiglucotoxicity effect by the knockdown of Nrf2 in RSC96 cells. In KK-Ay mice, we demonstrated that SalA could effectively improve the abnormal glucose and lipid metabolism and significantly protect against DPN by increasing the mechanical withdrawal threshold and sciatic nerve conduction velocity and restoring the ultrastructural impairment of the injured sciatic nerve induced by diabetes. Hence, SalA protected against DPN by antioxidative stress, attenuating neuroinflammation, and improving mitochondrial function via Nrf2. SalA may be prospective therapeutics for treating DPN.
- Published
- 2020
44. Phylogenetic, comparative genomic and structural analyses of human Streptococcus agalactiae ST485 in China
- Author
-
Ting Huang, Yan Huang, Rui Wang, Xiuying Yang, Weiyi Huang, Aiying Lei, Ming Chen, and Liping Li
- Subjects
0301 basic medicine ,Group B Streptococcus ,China ,Lac.2 ,lcsh:QH426-470 ,lcsh:Biotechnology ,030106 microbiology ,Cattle Diseases ,Genetic relationship ,medicine.disease_cause ,Genome ,Streptococcus agalactiae ,Evolution, Molecular ,03 medical and health sciences ,Cross infection ,Streptococcal Infections ,lcsh:TP248.13-248.65 ,CC103 ,Genetics ,medicine ,Animals ,Humans ,CadDX ,Gene ,Phylogeny ,Recombination, Genetic ,ST485 ,biology ,Phylogenetic tree ,Streptococcus ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,biology.organism_classification ,lcsh:Genetics ,030104 developmental biology ,bacteria ,Cattle ,DNA microarray ,Genome, Bacterial ,Bacteria ,Research Article ,Biotechnology - Abstract
Background Streptococcus agalactiae (Group B Streptococcus, GBS) is a common bacteria species infecting both human and bovine. Previous studies have shown that the GBS isolated from human and bovine are mostly unrelated and belong to separate populations. However, recently, the bovine GBS CC103 has become the dominant epidemic strain and frequently isolated from human patients. In particular, the ST485 GBS, a member of CC103, has become the new dominant ST in China and exhibited very high pathogenicity. This phenomenon is not consistent with the established understanding about the relationship between bovine and human GBS, which needs to be re-investigated. Results The genome-based phylogenetic analysis showed that the human and bovine GBS CC103 strains had very close genetic relationship and they were alternately distributed on the evolutionary tree. CC103 strains evolved into several branches, including the ST485, which exhibited high pathogenicity and specifically infected human. Compared to other CC103 strains, the ST485 lacked Lac.2 gene structure and acquired the CadDX gene structure in their genomes. Conclusions Our results indicate that GBS CC103 could propagate across human and bovine, and GBS ST485 might evolve from the ST103 that could infect both human and bovine. Moreover, the recombination of Lac.2 and CadDX gene structures might play an important role in the formation of highly pathogenic ST485 in China. Electronic supplementary material The online version of this article (10.1186/s12864-018-5084-0) contains supplementary material, which is available to authorized users.
- Published
- 2018
45. High-performance microwave absorption materials based on MoS 2 -graphene isomorphic hetero-structures
- Author
-
Wenjun Zhang, Xiuying Yang, Jia Yixuan, Deqing Zhang, Jixing Chai, Shengmei Chen, Hao Wang, Zhenlong Zhao, Zhiyu Wu, Guangping Zheng, Mao-Sheng Cao, Chen Han, and Junye Cheng
- Subjects
Materials science ,business.industry ,Graphene ,Mechanical Engineering ,Reflection loss ,Metals and Alloys ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Exfoliation joint ,0104 chemical sciences ,Nanomaterials ,law.invention ,Mechanics of Materials ,law ,Electrical resistivity and conductivity ,Specific surface area ,Materials Chemistry ,Optoelectronics ,0210 nano-technology ,business ,Absorption (electromagnetic radiation) ,Microwave - Abstract
Two-dimensional (2D) nanomaterials have become a new class of microwave absorption (MA) materials due to their high specific surface area and peculiar electronic properties. In this study, MoS 2 /graphene 2D hybrid nanosheets with isomorphic hetero-structures are prepared by liquid exfoliation and hydrothermal reaction. Their chemical composition and morphology are characterized and their MA properties are also investigated comprehensively. The minimum reflection loss (RL) value of an absorber containing wax and 20 wt% MoS 2 /graphene hybrid nanosheets (MoS 2 /GN) is −55.3 dB at a thickness of only 1.6 mm. The largest effective absorption bandwidth is up to 5.6 GHz for an absorber containing wax and 15 wt% MoS 2 /GN at a thickness of 2.2 mm. The enhanced MA performance of MoS 2 /GN is attributed to the high electrical conductivity of graphene and the multiple MoS 2 /graphene interfaces in the heterogeneous structures. The results suggest that those light-weight MoS 2 /graphene hybrid nanosheets are very promising materials for electromagnetic wave absorbing, which can exhibit broad effective absorption bandwidth in the absorber containing low loadings of MA materials at a small thickness. Besides, the facile preparation of MoS 2 /graphene hybrid nanosheets reported in this work provides a feasible and effective approach in the synthesis of high-performance 2D MA nanomaterials.
- Published
- 2018
46. Morphology characterization and the phase separation behavior of UHMWPE/recycled-PA6 blends using FTIR imaging and thermomechanical analysis
- Author
-
Junye Cheng, Eryun Yan, Zhihua Yuan, Deqing Zhang, Wenbo Wang, and Xiuying Yang
- Subjects
Materials science ,Morphology (linguistics) ,Polymers and Plastics ,General Chemical Engineering ,Organic Chemistry ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Characterization (materials science) ,Chemical engineering ,Thermomechanical analysis ,Fourier transform infrared spectroscopy ,0210 nano-technology - Published
- 2018
47. Glucose consumption assay discovered a novel compound from natural product with beneficial effects on diabetic mice via AMPK activation
- Author
-
Lili Shi, Guifen Qiang, Lin Yin, Wei-hua Jia, Guanhua Du, Xiuying Yang, Lianhua Fang, Chunyang Xu, Xi Chen, and Li Zhang
- Subjects
Consumption (economics) ,chemistry.chemical_compound ,Natural product ,chemistry ,Applied Mathematics ,General Mathematics ,AMPK ,Diabetic mouse ,Pharmacology ,Beneficial effects - Published
- 2018
48. Isolation of human GBS CC67 strains with high adhesion ability to HBMECs
- Author
-
J. Hu, Y. Huang, Xiuying Yang, M. Chen, T. Huang, Lanjuan Li, Yunning Liu, and R. Wang
- Subjects
Microbiology (medical) ,Isolation (health care) ,Brain ,Endothelial Cells ,General Medicine ,Biology ,Models, Biological ,Bacterial Adhesion ,Streptococcus agalactiae ,Microbiology ,Infectious Diseases ,Streptococcal Infections ,High adhesion ,Humans - Published
- 2019
49. AIE-ESIPT based colorimetric and 'OFF-ON-OFF' fluorescence Schiff base sensor for visual and fluorescent determination of Cu2+ in an aqueous media
- Author
-
Longshan Zhao, Lei Wu, Ning Liang, Yousheng Wang, Wenyue Pan, and Xiuying Yang
- Subjects
Detection limit ,Schiff base ,Aqueous solution ,General Chemical Engineering ,Metal ions in aqueous solution ,General Physics and Astronomy ,General Chemistry ,Photochemistry ,Fluorescence ,chemistry.chemical_compound ,chemistry ,Tap water ,Density functional theory ,Stoichiometry - Abstract
A novel benzimidazole-derived active Schiff base fluorescence probe 1-[[(1H-1,3-benzodiazol-2-yl)imino]methyl]naphthalen-2-ol (HBN) was designed, synthesized and characterized with high selectivity toward Cu2+ based on “OFF-ON-OFF” aggregation-induced emission (AIE) fluorescence strategy. HBN exhibited strong fluorescence enhancement in water. It was used for the selective determination of Cu2+ in aqueous solution, which displayed a significant fluorescence quenching in CH3CH2OH-PBS (v/v = 1:1, pH = 7.0) mixed solution. HBN displayed a good linear relationship with Cu2+ in the range of 0.16–250 μM and the limit of detection was 8.14 × 10−8 M. The binding stoichiometry of HBN with Cu2+ was 2:1 calculating by Job’s plot analysis, FT-IR and density functional theory. Ultimately, in order to verify the paracticability of the synthesized probe, HBN was applied to the detection of Cu2+ in tap water, traditional Chinese medicinal materials and marine products. The above description implied the good application prospect of detecting Cu2+ in the complicated environment, and provided a reference for the identification of metal ions.
- Published
- 2021
50. Effective nondestructive evaluations on UHMWPE/Recycled-PA6 blends using FTIR imaging and dynamic mechanical analysis
- Author
-
Zhihua Yuan, Xiuying Yang, Junye Cheng, Guangping Zheng, Hao Wang, Deqing Zhang, Shuilin Wu, Wenjun Zhang, Liubing Dong, Shengmei Chen, and Wenbo Wang
- Subjects
chemistry.chemical_classification ,Materials science ,Polymers and Plastics ,Organic Chemistry ,02 engineering and technology ,Polymer ,Dynamic mechanical analysis ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Microstructure ,01 natural sciences ,Miscibility ,0104 chemical sciences ,symbols.namesake ,Fourier transform ,chemistry ,Phase (matter) ,symbols ,Polymer blend ,Fourier transform infrared spectroscopy ,Composite material ,0210 nano-technology - Abstract
The noninvasive and nondestructive Fourier transform infrared (FTIR) imaging and dynamic mechanical analysis (DMA) are developed in this work to evaluate the microstructure-related properties of UHMWPE/Recycled-PA6 blends. FTIR imaging which is of in-situ and visualizing capabilities is shown to be extremely valuable in determining the phase structure of a multiphase system. It is found that small quantities of the HDPE-g-MAH compatibilizer could significantly improve the miscibility of the two immiscible polymers. It is further proved that in the UHMWPE/R-PA6 blends the R-PA6 phase distributes continuously, while the UHMWPE phase disperses in a discontinuous manner. Moreover, the blends with 44 wt% R-PA6 is found to exhibit an optimal miscibility behavior. This work demonstrates that FTIR imaging is a direct method in visualizing the miscibility of polymer blends. The combined FTIR imaging and DMA testing offers a new approach for qualitative and quantitative investigations on polymer blends with complex microstructures.
- Published
- 2017
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.