41 results on '"Xiu-Mei Wang"'
Search Results
2. Case Report: Cavernous hemangioma of rib: an extremely rare venous malformation but easily misdiagnosed as aggressive tumors
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Shan Liu, Luan Xiang, Fan Ding, Ling Yuan, and Xiu-Mei Wang
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hemangioma ,rib ,computerized tomography ,malignancy tumor ,case report ,venous bony malformation ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Hemangioma is a congenital vascular malformation that occurs commonly in the skin and soft tissues of younger individuals but rarely in the bone. The term hemangioma occurring in bone has been referred to as venous bony malformation also. In these rare cases, vertebral bodies occur more often, followed by the craniofacial skeleton and long bones. Most rib tumors are malignant, and hemangiomas of the rib tend to grow expansively and disrupt the cortex. Venous malformations in ribs are not tumors but can be misdiagnosed as aggressive tumors or infectious processes. In fact, hemangioma of bone is a locally aggressive benign vascular malformation associated with a good prognosis. To date, no more than 50 cases of rib hemangiomas have been described in the English literature. This report presents a case of an asymptomatic 27-year-old female patient who found a quail egg-sized lump on the right side of her chest that was the size of a cocoon 2 months prior. Then, the lump grew rapidly to the size of an egg when she presented to the hospital. Because of its worrisome histomorphologic features and aggressive clinical radiologic findings, it was once misdiagnosed as a malignant tumor by most doctors. However, the pathological results after the operation confirmed rib hemangioma. Therefore, this case report aims to share this particular case so that more doctors can better understand the particularity of this disease’s progression.
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- 2023
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3. Construction and Application of Standardized Postoperative Pain-Management Procedure for Patients With Perianal Abscess: A Retrospective Study
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Xiu-Mei Wang, Wei-Lian Jiang, Li-Fang Ma, Yue Guo, Li-Ping Cui, and Yan-Bin Niu
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perianal abscess ,pain ,standardized procedures ,nursing ,postoperative pain ,Surgery ,RD1-811 - Abstract
ObjectiveThe present study explored the construction and application of a standardized postoperative pain-management procedure for patients with perianal abscess.MethodsTwo study groups (the observation group and the intervention group) were established retrospectively. The observation group comprised 46 patients with perianal abscess who enrolled in this study between June 2019 and June 2020. The intervention group comprised 48 patients who enrolled in the study between July 2020 and July 2021. All patients were enrolled using the convenience sampling method. A pain-management team was established, and standardized procedure management was implemented in the intervention group, while routine pain management was implemented in the observation group. Indices related to the patients' postoperative pain-control satisfaction and rehabilitation were compared between the two groups.ResultsThe patients' pain-control satisfaction, wound edema score, edema disappearance time, urinary retention, and defecation difficulty following intervention were better in the intervention group than in the observation group, and the differences were statistically significant (P
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- 2022
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4. Tanshinone IIA improves functional recovery in spinal cord injury-induced lower urinary tract dysfunction
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Yong-dong Yang, Xing Yu, Xiu-mei Wang, Xiao-hong Mu, and Feng He
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nerve regeneration ,spinal cord injury ,tanshinone IIA ,spinal pathway ,lower urinary tract dysfunction ,neurogenic bladder ,dorsal root ganglion ,detrusor-sphincter dyssynergia ,urodynamics ,neural regeneration ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Tanshinone IIA, extracted from Salvia miltiorrhiza Bunge, exerts neuroprotective effects through its anti-inflammatory, anti-oxidative and anti-apoptotic properties. This study intravenously injected tanshinone IIA 20 mg/kg into rat models of spinal cord injury for 7 consecutive days. Results showed that tanshinone IIA could reduce the inflammation, edema as well as compensatory thickening of the bladder tissue, improve urodynamic parameters, attenuate secondary injury, and promote spinal cord regeneration. The number of hypertrophic and apoptotic dorsal root ganglion (L6–S1) cells was less after treatment with tanshinone IIA. The effects of tanshinone IIA were similar to intravenous injection of 30 mg/kg methylprednisolone. These findings suggested that tanshinone IIA improved functional recovery after spinal cord injury-induced lower urinary tract dysfunction by remodeling the spinal pathway involved in lower urinary tract control.
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- 2017
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5. Mineralized Collagen: Rationale, Current Status, and Clinical Applications
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Zhi-Ye Qiu, Yun Cui, Chun-Sheng Tao, Zi-Qiang Zhang, Pei-Fu Tang, Ke-Ya Mao, Xiu-Mei Wang, and Fu-Zhai Cui
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mineralized collagen ,collagen ,hydroxyapatite ,biomineralization ,self-assembly ,medical device product ,clinical application ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
This paper presents a review of the rationale for the in vitro mineralization process, preparation methods, and clinical applications of mineralized collagen. The rationale for natural mineralized collagen and the related mineralization process has been investigated for decades. Based on the understanding of natural mineralized collagen and its formation process, many attempts have been made to prepare biomimetic materials that resemble natural mineralized collagen in both composition and structure. To date, a number of bone substitute materials have been developed based on the principles of mineralized collagen, and some of them have been commercialized and approved by regulatory agencies. The clinical outcomes of mineralized collagen are of significance to advance the evaluation and improvement of related medical device products. Some representative clinical cases have been reported, and there are more clinical applications and long-term follow-ups that currently being performed by many research groups.
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- 2015
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6. DihydrotanshinoneⅠ promotes apoptosis of human esophageal squamous cell carcinoma cell line EC9706
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WANG Xiu-mei, WANG Kang-kang, CHEN Xiao-yan, LUO Jin-jian
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dihydrotanshinoneⅰ ,esophageal squamous cell carcinoma cell line ec9706 ,wnt/β-catenin signaling pathway ,Medicine - Abstract
Objective To investigate whether dihydrotanshinone Ⅰ could regulate the biological activities of proliferation, migration and apoptosis of esophageal squamous cell carcinoma cell line EC9706 through Wnt/β-catenin signaling pathway. Methods EC9706 cells were cultured in medium containing 0, 10, and 30 μmol/L dihydrotanshinoneⅠ solution, and cell proliferation was examined with a cell counting kit-8, cell migration was checked with scratch test. Western blot was used to detect β-catenin, cyclin D1 and c-myc protein expression in cells. Apoptosis was detected by flow cytometry. Results DihydrotanshinoneⅠ effectively inhibited the proliferation and migration of EC9706 cells. DihydrotanshinoneⅠ down-regulated β-catenin, cyclin D1 and c-myc protein expression in cells (P<0.05). DihydrotanshinoneⅠ promoted apoptosis in a dose-dependent manner. Conclusions DihydrotanshinoneⅠ can inhibit proliferation and migration of EC9706 cells and promote the cells apoptosis.
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- 2020
7. Predictors and occurrence of postoperative cognitive dysfunction in children undergoing noncardiac surgery: A prospective cohort study
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Fang‐Fang Han, Xiu‐Mei Wang, Hai‐Jun Zhang, Jun‐Ze Wang, Zhen‐Xing Bao, and Yu‐Lan Li
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- 2022
8. Arbutin suppresses osteosarcoma progression via miR‐338‐3p /MTHFD1L and inactivation of the AKT/mTOR pathway
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Cheng-Qun Wang, Yuan-Min Zhang, Lei Wang, Xiu-Mei Wang, and Bing-Liang Li
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0301 basic medicine ,Cell Survival ,proliferation ,AKT/mTOR ,Bone Neoplasms ,General Biochemistry, Genetics and Molecular Biology ,Formate-Tetrahydrofolate Ligase ,MTHFD1L ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cyclin D1 ,Aminohydrolases ,Cell Movement ,Multienzyme Complexes ,Cell Line, Tumor ,Humans ,Viability assay ,Protein kinase B ,Research Articles ,miR‐338‐3p ,PI3K/AKT/mTOR pathway ,Methylenetetrahydrofolate Dehydrogenase (NADP) ,Osteosarcoma ,Kinase ,Cell growth ,TOR Serine-Threonine Kinases ,Arbutin ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,chemistry ,Cell culture ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Cancer research ,Proto-Oncogene Proteins c-akt ,Research Article ,Signal Transduction - Abstract
Arbutin, a natural polyphenol, inhibits OS cell viability, invasion and migration by down‐regulating MTHFD1L expression. miR‐338‐3p directly targets MTHFD1L. This regulatory action is associated with the inactivation of the AKT/mTOR signaling pathway., Arbutin, a glycoside extracted from the plant Arctostaphylos uva‐ursi, has been previously reported to possess antioxidant, anti‐inflammatory and anticancer effects. Here, we investigated whether arbutin affects the proliferation of the cells of the osteosarcoma (OS) cell lines MG‐63 and SW1353. Arbutin suppressed OS cell viability in a dose‐ and time‐dependent manner, as shown by Cell Counting Kit‐8 assay. Furthermore, arbutin exposure decreased the protein levels of MTHFD1L, CCND1 and phosphorylated‐protein kinase B (AKT)/phosphorylated‐mammalian target of rapamycin (mTOR). Potential upstream miRNAs of MTHFD1L were predicted using TargetScan, PICTAR5, miRanda and miRWalk. We performed luciferase activity assays to show that miR‐338‐3p directly targets and negatively regulates the expression of MTHFD1L. Knockdown of miR‐338‐3p promoted cell invasion, migration and proliferation in arbutin‐treated OS cells via MTHFD1L. In summary, our data suggest that arbutin inhibits OS cell proliferation, migration and invasion via miR‐338‐3p/MTHFD1L and by inactivating the AKT/mTOR pathway.
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- 2020
9. All-Purpose Nanostrategy Based on Dose Deposition Enhancement, Cell Cycle Arrest, DNA Damage and ROS Production as Prostate Cancer Radiosensitizer for Potential Clinical Translation
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Xiao-xiao Guo, Zhen-hu Guo, Meng Wu, Jing-song Lu, Wen-sheng Xie, Qiu-zi Zhong, Yong-jie Chi, Xiao-dan Sun, Xiu-mei Wang, Jian-ye Wang, Ming Liu, and Lingyun Zhao
- Abstract
Background Radiotherapy (RT) is one of the main treatments for men with prostate cancer (PCa). Yet, to date, with numerous sophisticated nano-formulations as radiosensitizers have been synthesized with inspiring therapeutic effect both in vitro and in vivo, there still lacks the successful clinical translation of such nanosystems. Meanwhile, almost all the attention has been paid on the enhanced dose deposition effect by secondary electrons of nanomaterials with high atomic numbers (Z), despite that cell-cycle arrest, DNA damage and also reactive oxygen species (ROS) production are critical working mechanisms accounting for radiosensitization. Methods Herein, an ‘all-purpose’ nanostrategy based on dose deposition enhancement, cell cycle arrest and ROS production as prostate cancer radiosensitizer for potential clinical translation was proposed. The rather simple structure of docetaxel loaded Au nanoparticles (NPs) with prostate specific membrane antigen (PSMA) ligand conjugation have been successfully synthesized by a rather facile protocol. Results Enhanced cellular uptake achieved via selective internalization of the NPs by PCa cells with positive PSMA expression could guarantee the enhanced dose deposition. Moreover, the as-synthesized nanosystem could arrest cell cycle at G2/M phases, which would reduce the ability of DNA damage repair for more irradiation sensitive of the PCa cells. Meanwhile, G2/M phases arrest would further promote cascade retention and enrichment of the NPs within the cells. Furthermore, ROS generation and double strand breaks greatly promoted by the NPs under irradiation (IR) could also provide an underlying basis for effective radiosensitizers. Conclusions Investigations from in vitro and in vivo confirmed the as-synthesized NPs as an effective nano-radiosensitizer with ideal safety. More importantly, all the moieties within the present nanosystem have been approved by FDA for the purpose of PCa treatment, thus making the it highly attractive for clinical translation.
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- 2021
10. Role and mechanisms of action of microRNA-21 as regards the regulation of the WNT/β-catenin signaling pathway in the pathogenesis of non-alcoholic fatty liver disease
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Xiu‑Mei Wang, Lei Shi, Chang‑Ping Li, Xiao‑Yi Wang, Mu‑Han Lü, Yu‑Mei Huang, and Xia Chen
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0301 basic medicine ,LRP6 ,Choline ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Methionine ,Downregulation and upregulation ,Genetics ,Animals ,Humans ,Protein kinase A ,Transcription factor ,Wnt Signaling Pathway ,beta Catenin ,Glycogen Synthase Kinase 3 beta ,Oncogene ,biology ,Chemistry ,Wnt signaling pathway ,non-alcoholic fatty liver disease ,Antagomirs ,General Medicine ,Articles ,WNT/β-catenin signaling pathway ,Cell biology ,Fatty acid synthase ,Disease Models, Animal ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Low Density Lipoprotein Receptor-Related Protein-6 ,biology.protein ,Signal transduction ,microRNA-21 - Abstract
The aim of the present study was to investigate the role of microRNA-21 (miR-21) in regulating the classical WNT/β-catenin signaling pathway by targeting low-density lipoprotein-related receptor 6 (LRP6) in non-alcoholic fatty liver disease (NAFLD). For this purpose, we established a NAFLD model by feeding C57BL/6J mice a methionine-choline-deficient diet. Antagomir-21 was then injected via the tail vein, and the expression levels of WNT/β-catenin signaling pathway-related proteins, such as LRP6, glycogen synthase kinase-3β (GSK3β), p-β-catenin, β-catenin and the downstream protein, peroxisome proliferator-activated receptor γ (PPAR-γ), and lipid metabolism-related genes, including sterol regulatory element-binding transcription factor 1c (SREBP1c), fatty acid synthase (FAS), carnitine palmitoyl transferase 1α (CPT1α) and adenosine 5-monophosphate (AMP)-activated protein kinase α (AMPKα), were detected. The results revealed that in the NAFLD model, LRP6 expression was negatively associated with miR-21 expression. After antago-nizing the expression of miR-21, the protein level of LRP6 was increased. In addition, the WNT/β-catenin signaling pathway was activated, and lipid accumulation and inflammation were alleviated in the liver. However, the expression of PPAR-γ was not inhibited following the upregulation of the WNT signaling pathway. Taken together, the results of this study demonstrate that the inhibition of miR-21 expression can alleviate NAFLD by targeting LRP6 to activate the WNT/β-catenin signaling pathway.
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- 2019
11. Histological Changes of Cervical Disc Tissue in Patients with Degenerative Ossification
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Yang Xiong, Ying-Li Yang, Yu-Shan Gao, Xiu-Mei Wang, and Xing Yu
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General Neuroscience ,Surgery ,Neurology (clinical) - Abstract
Objective : To explore the histological feature of the cervical disc degeneration in patients with degenerative ossification (DO) and its potential mechanisms.Methods : A total of 96 surgical segments, from cervical disc degenerative disease patients with surgical treatment, were divided into ossification group (group O, n=46) and non-ossification group (group NO, n=50) based on preoperative radiological exams. Samples of disc tissues and osteophytes were harvested during the decompression operation. The hematoxylin-eosin staining, Masson trichrome staining and Safranin O-fast green staining were used to compare the histological differences between the two groups. And the distribution and content of transforming growth factor (TGF)-β1, p-Smad2 and p-Smad3 between the two groups were compared by a semi-quantitative immunohistochemistry (IHC) method.Results : For all the disc tissues, the content of disc cells and collagen fibers decreased gradually from the outer annulus fibrosus (OAF) to the central nucleus pulposus (NP). Compared with group NO, the number of disc cells in group O increased significantly. But for proteoglycan in the inner annulus fibrosus (IAF) and NP, the content in group O decreased significantly. IHC analysis showed that TGF-β1, p-Smad2, and p-Smad3 were detected in all tissues. For group O, the content of TGF-β1 in the OAF and NP was significantly higher than that in group NO. For p-Smad2 in IAF and p-Smad3 in OAF, the content in group O were significantly higher than group NO.Conclusion : Histologically, cervical disc degeneration in patients with DO is more severe than that without DO. Local higher content of TGF-β1, p-Smad2, and p-Smad3 are involved in the disc degeneration with DO. Further studies with multi-approach analyses are needed to better understand the role of TGF-β/Smads signaling pathway in the disc degeneration with DO.
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- 2021
12. Novel combined shield design for eye and face protection from COVID-19
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Xiu-Mei Wang, Xiu-Ling Huang, Yu-Xiang Sun, Shui-Miao Du, Zi-Kai Hua, Yi-Wen Chen, and Jin-Rong Yang
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Face shield ,business.product_category ,Coronavirus disease 2019 (COVID-19) ,Polymers and Plastics ,Three-dimensional (3D) printing ,Mechanical Engineering ,Face (sociological concept) ,COVID-19 ,Computer security ,computer.software_genre ,World health ,Article ,Industrial and Manufacturing Engineering ,Crowds ,Mechanics of Materials ,Shield ,Business ,Goggles ,Inefficiency ,Personal protective equipment ,computer - Abstract
The World Health Organization emphasized the importance of goggles and face shields for protection of medical personnel at the outbreak of the COVID-19 pandemic. Unsurprisingly, almost all countries suffered from a critical supply shortage of goggles and face shields, as well as many other types of personal protective equipment (PPE), for a long period, owing to the lack of key medical material supplies and the inefficiency of existing fabrication methods arising from the need to avoid crowds during the outbreak of COVID-19. In this paper, we propose a novel combined shield design for eye and face protection that can be rapidly fabricated using three-dimensional printing technology. The designed prototype eye-face shield is accessible to the general public, offering more possibilities for yield improvement in PPE during emergent infectious disease events such as COVID-19.
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- 2021
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13. A Novel Combinatorial Shield Design for Eye and Face Protection in COVID-19
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Xiu-Mei Wang, Jin-Rong Yang, Yu-Xiang Sun, Xiu-Ling Huang, Yi-Wen Chen, Zi-Kai Hua, and Shui-Miao Du
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Coronavirus disease 2019 (COVID-19) ,business.industry ,Computer science ,Shield ,Face (geometry) ,Computer vision ,Artificial intelligence ,business - Abstract
The World Health Organization emphasized the importance of goggles and face shields for protection of medical personnel at the outbreak of the COVID-19 pandemic. Unsurprisingly, almost all countries suffered from a critical supply shortage of goggles and face shields, as well as many other types of personal protective equipment (PPE), for a long period, owing to the lack of key medical material supplies and the inefficiency of existing fabrication methods arising from the need to avoid crowds during the outbreak of COVID-19. In this context, we propose a novel combined shield design for eye and face protection that can be rapidly fabricated using three-dimensional printing technology. The designed prototype eye–face shield is accessible to the general public , offering more possibilities for yield improvement in PPE during emergent infectious disease events such as COVID-19.
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- 2020
14. Histological Changes of Cervical Disc Tissue in Patients with Degenerative Ossification.
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Yang Xiong, Ying-Li Yang, Yu-Shan Gao, Xiu-Mei Wang, and Xing Yu
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INTERVERTEBRAL disk ,OSSIFICATION ,TRANSFORMING growth factors ,NUCLEUS pulposus ,SURGICAL therapeutics - Abstract
Objective: To explore the histological feature of the cervical disc degeneration in patients with degenerative ossification (DO) and its potential mechanisms. Methods: A total of 96 surgical segments, from cervical disc degenerative disease patients with surgical treatment, were divided into ossification group (group O, n=46) and non-ossification group (group NO, n=50) based on preoperative radiological exams. Samples of disc tissues and osteophytes were harvested during the decompression operation. The hematoxylin-eosin staining, Masson trichrome staining and Safranin O-fast green staining were used to compare the histological differences between the two groups. And the distribution and content of transforming growth factor (TGF)-β1, p-Smad2 and p-Smad3 between the two groups were compared by a semi-quantitative immunohistochemistry (IHC) method. Results: For all the disc tissues, the content of disc cells and collagen fibers decreased gradually from the outer annulus fibrosus (OAF) to the central nucleus pulposus (NP). Compared with group NO, the number of disc cells in group O increased significantly. But for proteoglycan in the inner annulus fibrosus (IAF) and NP, the content in group O decreased significantly. IHC analysis showed that TGF-β1, p-Smad2, and p-Smad3 were detected in all tissues. For group O, the content of TGF-β1 in the OAF and NP was significantly higher than that in group NO. For p-Smad2 in IAF and p-Smad3 in OAF, the content in group O were significantly higher than group NO. Conclusion: Histologically, cervical disc degeneration in patients with DO is more severe than that without DO. Local higher content of TGF-β1, p-Smad2, and p-Smad3 are involved in the disc degeneration with DO. Further studies with multi-approach analyses are needed to better understand the role of TGF-β/Smads signaling pathway in the disc degeneration with DO. [ABSTRACT FROM AUTHOR]
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- 2022
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15. Analysis of the levels of in vivo immunological indicators in patients with different types of malignanat lymphoma
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Xiu-Mei Wang, Lin Wang, and Yong-Mei Liao
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lcsh:R ,lcsh:Medicine ,Malignant lymphoma Cellular immunity Humoral immunity T lymphocyte Immunoglobulin Complement - Abstract
Objective: To analyze the levels of in vivo immunological indicators in patients with different types of malignanat lymphoma. Methods: A total of 69 patients with different types of malignanat lymphoma who were admitted in our hospital were included in the study, among which 31 had primary extranodular malignant lymphoma (extranodular group), and 38 had primary nodular malignant lymphoma (nodular group). Moreover, 34 patients with pure lymphnoditis were served as the control group. FCM and full-automatic speed scattering turbidimetry were used to detect T lymphocyte subsets proportion, IgG, IgA, IgM, C3, and C4 in the three groups. Results: The comparison of CD3+, CD4+, CD8+, NK cell proportion, and CD4+/CD8+ among the three groups was significantly different. When compared with the control group, CD3+, CD4+, CD8+, NK cell proportion, and CD4+/CD8+ in the extranodular group and nodular group were significantly reduced; moreover, CD3+, CD4+, CD8+, and CD4+/ CD8+ in the extranodular group were significantly lower than those in the nodular group, while NK cell in the extranodular group was significantly higher than that in the nodular group. The comparison of IgG, IgA, and C3 levels among the three groups was statistically significant. When compared with the control group, IgG and IgA levels in the extranodular group and nodular group were significantly elevated, while C3 level was significantly reduced, and the comparison between the extranodular group and nodular group between the two groups was statistically significant. IgM level in the extranodular group and nodular group was elevated, C4 level was reduced, but the comparison with the control group was not statistically significant. Spearman correlation analysis showed that CD3+ and CD4+ were significantly correlated with IgA, IgG, and C3. Conclusions: With the increasing of severity degree of lymphoma, the immunological level is reduced, while CD3+ and CD4+ are significantly correlated with IgA, IgG, and C3, indicating that detection of CD3+ and CD4+ proportion or IgA, IgG, and C3 contents in combined with pathological characteristics can evaluate the severity degree of malignant lymphoma in order to provide guidance for the clinical diagnosis and treatment.
- Published
- 2017
16. Naringin protects human adipose-derived mesenchymal stem cells against hydrogen peroxide-induced inhibition of osteogenic differentiation
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Yu-ge Zhang, Xiu-Mei Wang, Yuan-Min Zhang, Long-Fei Ma, and Lei Wang
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medicine.medical_specialty ,Cell Survival ,Cellular differentiation ,Toxicology ,medicine.disease_cause ,chemistry.chemical_compound ,Osteogenesis ,Internal medicine ,medicine ,Humans ,Wnt Signaling Pathway ,Naringin ,Osteoblasts ,Mesenchymal stem cell ,Wnt signaling pathway ,Cell Differentiation ,Mesenchymal Stem Cells ,Hydrogen Peroxide ,General Medicine ,Cytoprotection ,Cell biology ,RUNX2 ,Oxidative Stress ,Endocrinology ,Adipose Tissue ,chemistry ,Flavanones ,Alkaline phosphatase ,Oxidative stress - Abstract
Extensive evidence indicates that oxidative stress plays a pivotal role in the development of osteoporosis. We show that naringin, a natural antioxidant and anti-inflammatory compound, effectively protects human adipose-derived mesenchymal stem cells (hADMSCs) against hydrogen peroxide (H2O2)-induced inhibition of osteogenic differentiation. Naringin increased viability of hAMDSCs and attenuated H2O2-induced cytotoxicity. Naringin also reversed H2O2-induced oxidative stress. Oxidative stress induced by H2O2 inhibits osteogenic differentiation by decreasing alkaline phosphatase (ALP) activity, calcium content and mRNA expression levels of osteogenesis marker genes RUNX2 and OSX in hADMSCs. However, addition of naringin leads to a significant recovery, suggesting the protective effects of naringin against H2O2-induced inhibition of osteogenic differentiation. Furthermore, the H2O2-induced decrease of protein expressions of β-catenin and clyclin D1, two important transcriptional regulators of Wnt-signaling, was successfully rescued by naringin treatment. Also, in the presence of Wnt inhibitor DKK-1, naringin is no longer effective in stimulating ALP activity, increasing calcium content and mRNA expression levels of RUNX2 and OSX in H2O2-exposed hADMSCs. These data clearly demonstrates that naringin protects hADMSCs against oxidative stress-induced inhibition of osteogenic differentiation, which may involve Wnt signaling pathway. Our work suggests that naringin may be a useful addition to the treatment armamentarium for osteoporosis and activation of Wnt signaling may represent attractive therapeutic strategy for the treatment of degenerative disease of bone tissue.
- Published
- 2015
17. Correction to: Is cell transplantation a reliable therapeutic strategy for spinal cord injury in clinical practice? A systematic review and meta-analysis from 22 clinical controlled trials
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He Zhao, Qing-Ling Sun, Li-Jun Duan, Yong-Dong Yang, Yu-Shan Gao, Ding-Yan Zhao, Yang Xiong, He-Jun Wang, Jia-Wei Song, Kai-Tan Yang, Xiu-Mei Wang, and Xing Yu
- Subjects
Orthopedics and Sports Medicine ,Surgery - Published
- 2020
18. Advances in the surface modification techniques of bone-related implants for last 10 years
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Zhi-Ye Qiu, Xiu-Mei Wang, Cen Chen, and In-Seop Lee
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Plasma surface ,Materials science ,Biocompatibility ,Alloy ,ion beam processing ,technology, industry, and agriculture ,Reviews ,surface patterning ,bone-related materials ,chemistry.chemical_element ,Nanotechnology ,radiation grafting ,Polyethylene ,engineering.material ,Biomaterials ,chemistry.chemical_compound ,plasma surface engineering ,chemistry ,Coating ,Ion beam processing ,physicochemical coating ,engineering ,Surface modification ,surface modification ,Titanium - Abstract
At the time of implanting bone-related implants into human body, a variety of biological responses to the material surface occur with respect to surface chemistry and physical state. The commonly used biomaterials (e.g. titanium and its alloy, Co-Cr alloy, stainless steel, polyetheretherketone, ultra-high molecular weight polyethylene and various calcium phosphates) have many drawbacks such as lack of biocompatibility and improper mechanical properties. As surface modification is very promising technology to overcome such problems, a variety of surface modification techniques have been being investigated. This review paper covers recent advances in surface modification techniques of bone-related materials including physicochemical coating, radiation grafting, plasma surface engineering, ion beam processing and surface patterning techniques. The contents are organized with different types of techniques to applicable materials, and typical examples are also described.
- Published
- 2014
19. Plasmids of Diverse Inc Groups Disseminate the Fosfomycin Resistance Gene fosA3 among Escherichia coli Isolates from Pigs, Chickens, and Dairy Cows in Northeast China
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Xin Hua, Xiu-Mei Wang, Yao Zhu, Wanjiang Zhang, Stefan Schwarz, Zhimin Dong, Yanhe Zhang, and Siguo Liu
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0301 basic medicine ,Pharmacology ,030106 microbiology ,Biology ,Fosfomycin ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,Infectious Diseases ,Plasmid ,Antibiotic resistance ,medicine ,Pharmacology (medical) ,Gene ,Escherichia coli ,medicine.drug - Abstract
Thirty-nine fosfomycin-resistant Escherichia coli isolates carrying fosA3 were obtained from pigs, chickens, dairy cows, and staff in four northeastern provinces of China between June 2015 and April 2016. The fosA3 gene was colocated with bla CTX-M genes on conjugative plasmids of the incompatibility groups IncN ( n = 12), IncN-F33:A − :B − ( n = 2), IncF33:A − :B − ( n = 14), IncF14:A − :B − ( n = 2), and IncI1/sequence type 136 (ST136) ( n = 9). Four different genetic contexts of fosA3 were detected among the 39 E. coli isolates. Three potential epidemic plasmids circulated among E. coli strains from this region.
- Published
- 2017
20. Plasmids of Diverse Inc Groups Disseminate the Fosfomycin Resistance Gene
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Xiu-Mei, Wang, Zhimin, Dong, Stefan, Schwarz, Yao, Zhu, Xin, Hua, Yanhe, Zhang, Siguo, Liu, and Wan-Jiang, Zhang
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China ,Fosfomycin ,Swine ,Mechanisms of Resistance ,Escherichia coli Proteins ,Drug Resistance, Bacterial ,Escherichia coli ,Animals ,Cattle ,Microbial Sensitivity Tests ,Chickens ,Escherichia coli Infections ,Plasmids - Abstract
Thirty-nine fosfomycin-resistant Escherichia coli isolates carrying fosA3 were obtained from pigs, chickens, dairy cows, and staff in four northeastern provinces of China between June 2015 and April 2016. The fosA3 gene was colocated with blaCTX-M genes on conjugative plasmids of the incompatibility groups IncN (n = 12), IncN-F33:A−:B−(n = 2), IncF33:A−:B−(n = 14), IncF14:A−:B−(n = 2), and IncI1/sequence type 136 (ST136) (n = 9). Four different genetic contexts of fosA3 were detected among the 39 E. coli isolates. Three potential epidemic plasmids circulated among E. coli strains from this region.
- Published
- 2017
21. ISOTHERMAL ADSORPTION CHARACTERISTICS OF BIORETENTION MEDIA FOR FECAL Escherichia Coli.
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Ying MEI, Hang ZHOU, Xiao-Han ZHU, Peng ZHANG, Xiao-Fei LI, Yi-Ming ZUO, Xiu-Mei WANG, and Jun-Jiang BAO
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ESCHERICHIA coli ,FLY ash ,PATHOGENIC bacteria ,LANGMUIR isotherms ,ADSORPTION isotherms ,SOIL absorption & adsorption ,COLIFORMS ,RUNOFF - Abstract
Rainfall runoff contains a huge number of pathogenic bacteria that seriously deteriorate water quality. Bioretention is an effective approach to removal of pathogenic bacteria from rainwater. This study uses sandy soil, fly ash, slag, sandy soil+5% fly ash, and sandy soil+5% slag as media to evaluate the adsorption of fecal Escherichia coli. The mechanisms of the five media conform to the Langmuir's isotherm adsorption equation, and a pseudo-first-order kinetic model is adopted to reveal the absorption kinetics. [ABSTRACT FROM AUTHOR]
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- 2020
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22. Characterization of the IncA/C plasmid pSCEC2 from Escherichia coli of swine origin that harbours the multiresistance gene cfr
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Xiu-Mei Wang, Wanjiang Zhang, Xing-Ran Xu, Siguo Liu, Lei Dai, Hua-Jun Zheng, and Stefan Schwarz
- Subjects
Microbiology (medical) ,Swine ,Context (language use) ,Biology ,medicine.disease_cause ,Plasmid ,Bacterial Proteins ,Drug Resistance, Multiple, Bacterial ,Escherichia coli ,medicine ,Animals ,Pharmacology (medical) ,Insertion sequence ,Gene ,Original Research ,Pharmacology ,Genetics ,Escherichia coli Proteins ,Nucleic acid sequence ,Methyltransferases ,Amplicon ,Phosphoproteins ,Open reading frame ,Infectious Diseases ,DNA Transposable Elements ,Plasmids - Abstract
Objectives: To determine the complete nucleotide sequence of the multidrug resistance plasmid pSCEC2, isolated from a porcine Escherichia coli strain, and to analyse it with particular reference to the cfr gene region. Methods: Plasmid pSCEC2 was purified from its E. coli J53 transconjugant and then sequenced using the 454 GSFLX System. After draft assembly, predicted gaps were closed by PCR with subsequent sequencing of the amplicons. Results: Plasmid pSCEC2 is 135615 bp in size and contains 200 open reading frames for proteins of ≥100 amino acids. Analysis of the sequence of pSCEC2 revealed two resistance gene segments. The 4.4 kb cfr-containing segment is flanked by two IS256 elements in the same orientation, which are believed to be involved in the dissemination of the rRNA methylase genecfr. The other segment harbours the resistance genesfloR,tet(A)-tetR,strA/strB andsul2, which have previously been found on other IncA/C plasmids. Except for these two resistance gene regions, the pSCEC2 backbone displayed .99% nucleotide sequence identity to that of other IncA/C family plasmids isolated in France, Chile and the USA. Conclusions: The cfr gene was identified on an IncA/C plasmid, which is well known for its broad host range and transfer and maintenance properties. The location on such a plasmid will further accelerate the dissemination of cfr and co-located resistance genes among different Gram-negative bacteria. The genetic context of cfr on plasmid pSCEC2 underlines the complexity of cfr transfer events and confirms the role that insertion sequences play in the spread of cfr.
- Published
- 2013
23. An inhibitor of cathepsin K, icariin suppresses cartilage and bone degradation in mice of collagen-induced arthritis
- Author
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Xiaoxia Deng, Wei Si, Xiu-Mei Wang, Yonggang Liu, Liping Chen, Peijiao Sun, Xianglian Yuan, Siguo Liu, Defeng Wu, Shenye Yu, Nasang Dai, Chunmei Yu, and Hai Pang
- Subjects
Deoxypyridinoline ,medicine.medical_specialty ,Cathepsin K ,Type II collagen ,Pharmaceutical Science ,Arthritis ,Cartilage Oligomeric Matrix Protein ,Bone and Bones ,Collagen Type I ,Arthritis, Rheumatoid ,Mice ,chemistry.chemical_compound ,N-terminal telopeptide ,Internal medicine ,Drug Discovery ,medicine ,Animals ,Amino Acids ,Collagen Type II ,Epimedium ,Flavonoids ,Pharmacology ,Cartilage oligomeric matrix protein ,Mice, Inbred BALB C ,biology ,Chemistry ,medicine.disease ,Arthritis, Experimental ,Cartilage ,Endocrinology ,Complementary and alternative medicine ,biology.protein ,Molecular Medicine ,Female ,Peptides ,Icariin ,Type I collagen ,Drugs, Chinese Herbal ,Phytotherapy - Abstract
The collagenase cathepsin K has been shown important in the pathogenesis of rheumatoid arthritis (RA). Icariin is the major pharmacologically active flavonol diglycoside of Herba Epimedii, an herb used in Chinese traditional medicine to treat arthritis. We investigated whether icariin can inhibit the protease activity of cathepsin K and its effects on a murine model of collagen-induced arthritis (CIA). Six-week old female BALB/C mice were immunized with type II collagen and treated with vehicle alone icariin (25mg/kg) for 21 days; a control remained untreated. Serum concentrations of type I collagen C-terminal telopeptide (CTX-I) and cartilage oligomeric matrix protein (COMP) and urinary concentrations of deoxypyridinoline (DPD) were measured, and disease severity was assessed. Compared with immunized, untreated mice, immunized icariin-treated mice had significantly lower urinary DPD (~25%, p
- Published
- 2013
24. Exploration on the safety assessment of nanomaterials in China
- Author
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Kun Hu, Xiu-Mei Wang, Xin-Li Shi, and Qiangbin Wang
- Subjects
business.industry ,Biomedical Engineering ,Biophysics ,Chinese market ,Bioengineering ,Articles ,Biochemistry ,Biomaterials ,Food and drug administration ,Risk analysis (engineering) ,Medicine ,business ,China ,Biotechnology - Abstract
More and more applications of nanomaterials have been achieved in the biomedicine field. Numerous nanomedical devices, such as bone grafts with nano-hydroxyapatite and the silver-based anti-bacteria products, have been developed and have been trying to enter into the Chinese market. The State Food and Drug Administration of China (SFDA) is facing a critical challenge of how to explore and supervise the safety assessment of the nanomedical products. This paper briefly introduces the approval status of nanomedical products and the current advances of the safety assessment of nanomaterials in China.
- Published
- 2012
25. Antimicrobial resistance, virulence genes, and phylogenetic background inEscherichia coli isolates from diseased pigs
- Author
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Rong Xiang, Hong-Xia Jiang, Jian-Hua Liu, Wan-Jiang Zhang, Xiu-Mei Wang, Ya-Hong Liu, Hao Zhang, Dian-Hong Lü, Xiao-Ping Liao, and Zhi-Gang Jiang
- Subjects
China ,Swine ,Virulence Factors ,Virulence ,Microbial Sensitivity Tests ,Drug resistance ,Biology ,medicine.disease_cause ,Microbiology ,Antibiotic resistance ,Drug Resistance, Bacterial ,Escherichia coli ,Genetics ,medicine ,Animals ,Cluster Analysis ,Molecular Biology ,Escherichia coli Infections ,Phylogeny ,Swine Diseases ,Molecular Epidemiology ,Antiinfective agent ,Polymorphism, Genetic ,Escherichia coli Proteins ,Sulfamethoxazole ,Kanamycin ,Virology ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Multiple drug resistance ,medicine.drug - Abstract
Escherichia coli isolates from diseased pigs were examined for antimicrobial susceptibility to 12 antimicrobials and possession of virulence genes (VGs), and then grouped according to the phylogenetic background and genetic relatedness. Associations between antimicrobial resistance (AMR) and VGs and between AMR and phylogenetic group were subsequently assessed. The results showed that most isolates (91%) were epidemiologically unrelated. Multiple antimicrobial-resistant phenotypes (or=5 antimicrobials) were observed in 89% of E. coli strains and the most frequent types of resistance were to sulfamethoxazole (95%), tetracycline (94%), chloramphenicol (89%), and streptomycin (84%). The majority of isolates belonged to phylogenetic group A (84%). The most prevalent VG was EAST1 (64%), followed by Stx2e (63%) and eae (47%). Resistance to ceftiofur was associated with the presence of certain VGs, whereas resistance to doxycycline and kanamycin was associated with the absence of certain VGs. These findings suggest that multidrug resistance phenotypes, a variety of VGs, and the clear associations between resistance and VGs are commonly present in E. coli strains from diseased pigs. These results indicate that there is a great need for surveillance programs in China to monitor AMR in pathogenic E. coli strains.
- Published
- 2010
26. Novel plasmid-borne multidrug resistance gene cluster including lsa(E) from a linezolid-resistant Enterococcus faecium isolate of swine origin
- Author
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Zhimin Dong, Xin Hua, Shengbo Chu, Stefan Schwarz, Hongbin Si, Wanjiang Zhang, Xiu-Mei Wang, Siguo Liu, and Lei Dai
- Subjects
Streptogramins ,Spectinomycin ,medicine.drug_class ,Swine ,Enterococcus faecium ,Microbial Sensitivity Tests ,Microbiology ,Mice ,Plasmid ,Mechanisms of Resistance ,Drug Resistance, Multiple, Bacterial ,Gene cluster ,medicine ,Animals ,Pharmacology (medical) ,Polycyclic Compounds ,Lincosamides ,Pharmacology ,biology ,Linezolid ,Kanamycin ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Anti-Bacterial Agents ,Infectious Diseases ,Streptomycin ,Macrolides ,Diterpenes ,medicine.drug ,Plasmids - Abstract
A novel nonconjugative plasmid of 28,489 bp from a porcine linezolid-resistant Enterococcus faecium isolate was completely sequenced. This plasmid harbored a novel type of multiresistance gene cluster that comprised the resistance genes lnu (B), lsa (E), spw , aadE , aphA3 , and two copies of erm (B), which account for resistance to macrolides, lincosamides, streptogramins, pleuromutilins, streptomycin, spectinomycin, and kanamycin/neomycin. Structural comparisons suggested that this plasmid might have developed from other enterococcal plasmids by insertion element (IS)-mediated interplasmid recombination processes.
- Published
- 2015
27. Novel conjugative plasmid from Escherichia coli of swine origin that coharbors the multiresistance gene cfr and the extended-spectrum-β-lactamase gene blaCTX-M-14b
- Author
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Xin Hua, Lei Dai, Xiu-Mei Wang, Siguo Liu, Wanjiang Zhang, Zhimin Dong, and Stefan Schwarz
- Subjects
Pharmacology ,Genetics ,Swine ,Conjugative plasmid ,Molecular Sequence Data ,Swine origin ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,beta-Lactamases ,Microbiology ,Infectious Diseases ,Plasmid ,Mechanisms of Resistance ,medicine ,Escherichia coli ,Animals ,Pharmacology (medical) ,Gene ,Antimicrobial Cationic Peptides ,Plasmids - Abstract
Two porcine Escherichia coli isolates harbored the cfr gene on conjugative plasmids of 38,405 bp (pGXEC6) and 41,646 bp (pGXEC3). In these two plasmids, the cfr gene was located within a 4,612-bp region containing a tnpA -IS 26-cfr -IS 26 -Δ hyp element. Plasmid pGXEC3 was almost identical to pGXEC6 except for a 3,235-bp IS Ecp1-bla CTX-M-14b insertion. The colocation of the multiresistance cfr gene with an extended-spectrum-β-lactamase gene on a conjugative plasmid may support the dissemination of these genes by coselection.
- Published
- 2014
28. Methicillin-resistant Staphylococcus aureus ST9 from a case of bovine mastitis carries the genes cfr and erm(A) on a small plasmid
- Author
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Siguo Liu, Huifang Liu, Wanjiang Zhang, Stefan Schwarz, Shenye Yu, Wei Si, Xiu-Mei Wang, and Rong-Min Zhang
- Subjects
DNA, Bacterial ,Methicillin-Resistant Staphylococcus aureus ,Microbiology (medical) ,Sequence analysis ,Molecular Sequence Data ,Biology ,medicine.disease_cause ,Microbiology ,Bacterial genetics ,chemistry.chemical_compound ,Plasmid ,Bacterial Proteins ,medicine ,Animals ,Pharmacology (medical) ,Mastitis, Bovine ,Gene ,Pharmacology ,Methyltransferases ,Sequence Analysis, DNA ,medicine.disease ,Methicillin-resistant Staphylococcus aureus ,Virology ,Mastitis ,Molecular Typing ,Infectious Diseases ,chemistry ,Horizontal gene transfer ,Cattle ,Female ,DNA ,Plasmids - Published
- 2012
29. Complete sequence of the bla(NDM-1)-carrying plasmid pNDM-AB from Acinetobacter baumannii of food animal origin
- Author
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Liping Chen, Rong-Min Zhang, Wanjiang Zhang, Zongji Lu, Wei Si, Xiu-Mei Wang, Stefan Schwarz, Siguo Liu, and Shenye Yu
- Subjects
Microbiology (medical) ,Acinetobacter baumannii ,DNA, Bacterial ,Swine ,Food animal ,Molecular Sequence Data ,Microbial Sensitivity Tests ,beta-Lactams ,Synteny ,beta-Lactam Resistance ,beta-Lactamases ,Microbiology ,Beta-lactam ,chemistry.chemical_compound ,Acinetobacter infections ,Complete sequence ,Plasmid ,Bacterial Proteins ,Gene Order ,Animals ,Pharmacology (medical) ,Carbapenem resistance ,Pharmacology ,Swine Diseases ,biology ,Sequence Analysis, DNA ,biology.organism_classification ,Infectious Diseases ,chemistry ,Carbapenems ,Acinetobacter Infections ,Plasmids - Published
- 2013
30. Validated HPLC–MS/MS Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma
- Author
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Xiu-Mei Wang, Jian Yang, Qi-Zhi Zhang, Wen-Xing Peng, Jun Zhang, Rong-Hua Zhu, and Li-Jing Cai
- Subjects
Detection limit ,Chromatography ,Chemistry ,Coefficient of variation ,Electrospray ionization ,Selected reaction monitoring ,Pharmaceutical Science ,Mass spectrometry ,High-performance liquid chromatography ,Curcumin, Piperine ,HPLC-MS/MS, Simultaneous determination ,Standard curve ,chemistry.chemical_compound ,Piperine ,Pharmacology (medical) - Abstract
Purpose: To develop a sensitive and rapid method for the simultaneous determination of curcumin and piperine in human plasma.Methods: The method was based on high-performance liquid chromatography (HPLC) with electrospray ionization tandem mass spectrometer (ESI-MS/MS) detection in positive ionization mode. The analytes and internal standard were isolated from acidified plasma using liquid–liquid extraction (LLE). The organic extracts were evaporated, reconstituted in mobile phase and injected into the HPLC-MS/MS system. The analytes were chromatographed on a XB-C8 analytical column and MS-MS detection was performed on an electrospray ionization tandem mass spectrometer operated in multiple reaction monitoring (MRM) mode. Precursor→product combinations of m/z 369.9→177.0, 286.3→201.1 and 285.6→193.1 were used to quantify curcumin, piperine and the internal standard (IS), respectively. The assay was validated in the concentration range of 1.0 – 100.0 ng/ml for curcumin and 0.5 – 800.0 ng/ml for piperine using 0.5 ml of plasma.Results: The lowest limit of quantification (LLOQ) for curcumin and piperine was 1.00 and 0.50 ng/ml, respectively. The precision of the assay (expressed as coefficient of variation, CV) was < 12.6 % at all concentrations within the standard curve range, with adequate assay accuracy. Stability data revealed that the drugs were stable in plasma under various test conditions.Conclusion: The method is highly selective and rugged for the estimation of curcumin and piperine in human plasma and would be applicable to toxicokinetic, pharmacokinetic, bioavailability, and bioequivalence studies.Keywords: Curcumin, Piperine; HPLC-MS/MS, Simultaneous determination
- Published
- 2012
31. Detection of mutations in the gyrA and parC genes in Escherichia coli isolates carrying plasmid-mediated quinolone resistance genes from diseased food-producing animals
- Author
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Bao-Tao Liu, Liang Li, Jian Sun, Liang-Xing Fang, Ya-Hong Liu, Lu-Lu Li, Yu-Rong Yang, Shou-Shen Yang, Dong-Hao Zhao, Xiu-Mei Wang, and Xiao-Ping Liao
- Subjects
Microbiology (medical) ,DNA Topoisomerase IV ,China ,Nalidixic acid ,medicine.drug_class ,Swine ,R Factors ,Drug resistance ,Biology ,Quinolones ,medicine.disease_cause ,Microbiology ,DNA gyrase ,Plasmid ,Drug Resistance, Multiple, Bacterial ,medicine ,Enrofloxacin ,Escherichia coli ,Animals ,Escherichia coli Infections ,Poultry Diseases ,Genetics ,Swine Diseases ,Escherichia coli Proteins ,General Medicine ,Gene Expression Regulation, Bacterial ,biochemical phenomena, metabolism, and nutrition ,Quinolone ,Anti-Bacterial Agents ,Ciprofloxacin ,Ducks ,DNA Gyrase ,Mutation ,Food Microbiology ,Chickens ,medicine.drug - Abstract
In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 Escherichia coli isolates from diseased food-producing animals in Guangdong province, China. The quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analysed for mutations in 55 isolates harbouring only oqxAB and all isolates harbouring other PMQR genes. Overall, 282 (57.0 %) E. coli isolates had at least one PMQR gene. oqxAB was detected in 215 isolates and predominated the PMQR genes, followed by qnrS (63 isolates), aac(6')-Ib-cr (56 isolates), qnrB (39 isolates) and qepA (18 isolates). qnrA, qnrC and qnrD were not found in any of the isolates. The rates of resistance to ciprofloxacin, enrofloxacin, levofloxacin and nalidixic acid were 75.2, 81.0, 70.5 and 97.4 %, respectively, among the 495 isolates. Eight types of mutation in gyrA were detected in 154 PMQR-positive isolates, and 147 isolates were found to have mutations in parC. PFGE analysis indicated that the PMQR-positive E. coli isolates were genetically diverse. This study demonstrated that the number of mutations in QRDRs of gyrA and/or parC was significantly associated with the MICs of quinolones (P
- Published
- 2012
32. Interaction of methylenetetrahydrofolate reductase C677T, cytochrome P4502E1 polymorphism and environment factors in esophageal cancer in Kazakh population
- Author
-
Lin He, Feng Li, Xiu-Mei Wang, Jiang-Mei Qin, Pei-Hua Liao, Bo Chen, and Lei Yang
- Subjects
Male ,Veterinary medicine ,China ,Alcohol Drinking ,Esophageal Neoplasms ,Population ,Kazakh ,Risk Factors ,Genotype ,medicine ,Humans ,Genetic Predisposition to Disease ,education ,Methylenetetrahydrofolate Reductase (NADPH2) ,Aged ,Retrospective Studies ,Genetics ,education.field_of_study ,Polymorphism, Genetic ,biology ,Malnutrition ,Water Pollution ,Gastroenterology ,Case-control study ,Cytochrome P-450 CYP2E1 ,General Medicine ,Esophageal cancer ,Middle Aged ,medicine.disease ,language.human_language ,Methylenetetrahydrofolate reductase ,Case-Control Studies ,biology.protein ,language ,Female ,Gene polymorphism ,Restriction fragment length polymorphism ,Rapid Communication - Abstract
AIM: To evaluate the association and interaction of genetic polymorphisms in methylenetetrahydrofolate reductase (MTHER) and cytochrome P4502E1 (CYP4502E1), environment risk factors with esophageal cancer (EC) in Kazakh, a high EC incidence area of Xinjiang Uygur Autonomous Region, China. METHODS: A 1:2 matched case-control study was conducted with 120 cases of EC and 240 population- or hospital-based controls. The controls were matched for sex, nationality, area of residence and age within a 5-year difference. MTHER and CYP4502E1 genotypes were identified by PCR-based restriction fragment length polymorphism (RFLP). A conditional logistic regression model was established to identify risk factors. The strata method was adopted in interaction analysis. RESULTS: Low consumption of green vegetables and fresh fruits, alcohol drinking, and unsafe water (shallow well, or river) were found to be the risk factors for EC. Individuals with the MTHFR677 (C/T + T/T) genotype had a 2.62-fold (95% CI: 1.61-4.28) risk of developing EC compared with those who carried the C/C genotype. Individuals with the CYP4502E1C1/C1 genotype had a 3.00-fold (95% CI: 1.82-4.96) risk compared with those who carried the CYP4502E1 (C1/C2 + C2/C2) genotype. Gene-environment interaction analysis showed that MTHFR677 gene polymorphism was correlated with consumption of green vegetables and fresh fruit, while CYP4502E1 C1/C1 was correlated with alcohol drinking and unsafe drinking water. MTHFR and CYP4502E1 analysis of gene-gene interaction showed that individuals with the MTHFR677 (C/T + T/T) and CYP4502E1C1/C1 genotypes had a 7.41-fold (95% CI: 3.60-15.25) risk of developing EC compared with those who carried the MTHFR677C/C and CYP4502E1 RsaI C1/C2 + C2/C2 genes, and the interaction rate was higher than that of the two factors alone. CONCLUSION: Low consumption of green vegetables and fresh fruits, alcohol drinking, and unsafe water (shallow well, or river) and polymorphisms in MTHFR and CYP4502E1 genes are important risk factors for EC. There is a synergistic interaction among polymorphisms in MTHFR and CYP4502E1 genes and environment factors. MTHFR and CYP4502E1 genes can be used as biomarkers for prevention of EC in Kazakh, Xinjiang Uygur Autonomous Region, China.
- Published
- 2008
33. Plasmid-mediated quinolone resistance determinants oqxAB and aac(6')-Ib-cr and extended-spectrum -lactamase gene blaCTX-M-24 co-located on the same plasmid in one Escherichia coli strain from China
- Author
-
Xue-Ying Chen, Xiao-Ping Liao, Ya-Hong Liu, Heng-Qian Zhu, Bao-Tao Liu, Jian Sun, and Xiu-Mei Wang
- Subjects
DNA, Bacterial ,Microbiology (medical) ,China ,Quinolones ,beta-Lactams ,medicine.disease_cause ,beta-Lactamases ,Microbiology ,Plasmid ,Drug Resistance, Multiple, Bacterial ,Escherichia coli ,medicine ,Animals ,Pharmacology (medical) ,Gene ,Escherichia coli Infections ,Poultry Diseases ,Antibacterial agent ,Electrophoresis, Agar Gel ,Pharmacology ,biology ,Strain (chemistry) ,biology.organism_classification ,Enterobacteriaceae ,Anti-Bacterial Agents ,Multiple drug resistance ,Infectious Diseases ,Genes, Bacterial ,Chickens ,Bacteria ,Plasmids - Published
- 2011
34. Identification and Cloning of Tillering-Related Genes OsMAX1 in Rice.
- Author
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Xiu-mei, WANG, Yue-yang, LIANG, Ling, LI, Chang-wei, GONG, Hai-peng, WANG, Xiao-xi, HUANG, Shuang-cheng, LI, Qi-ming, DENG, Jun, ZHU, Ai-ping, ZHENG, Ping, LI, and Shi-quan, WANG
- Subjects
MOLECULAR cloning ,RICE genetics ,BIOSYNTHESIS ,TILLERING (Botany) ,PLANT hormones - Abstract
Tillering is an important agronomic trait which has a direct impact on plant type and grain yield. Strigolactones are a class of important phytohormones regulating rice tillering. ATMAX1 is an important gene involved in strigolactone biosynthesis through encoding the protein P450 in Arabidopsis . Based on sequence BLASTp, we identified five homologous genes of ATMAX1 in rice, i.e., OsMAX1a , OsMAX1b , OsMAX1c , OsMAX1d and OsMAX1e . Among them, OsMAX1a and OsMAX1e showed stable and high expression in rice tissues. In addition, we observed that OsMAX1a and OsMAX1e can rescue the branched phenotype and the influences caused by MAX1 mutation in Arabidopsis . Moreover, the expression of OsMAX1a and OsMAX1e can respond to phosphate deficiency and different phytohormones, especially GR24, a strigolactone analogue. Therefore, it is concluded that OsMAX1a and OsMAX1e are involved in the biosynthesis of strigolactones and regulated rice tillering. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
35. A New Classification Algorithm Based on Gaussian Process Latent Variable Model
- Author
-
Xin-Bo Gao, Jie Li, and Xiu-Mei Wang
- Subjects
Computer Networks and Communications ,Hardware and Architecture ,Gaussian process latent variable model ,Computer science ,Computer Graphics and Computer-Aided Design ,Algorithm ,Software - Published
- 2012
36. Relationship of CYP2E1 gene polymorphism and tobacco and alcohol consumption with susceptibility to esophageal cancer in Kazakh
- Author
-
Pei-Hua Liao, Yan-Qing Ma, Feng Li, Xiu-Mei Wang, Ling He, Jiang-Mei Qin, Bo Chen, and Lei Yang
- Subjects
Oncology ,medicine.medical_specialty ,CYP2E1 Gene ,business.industry ,Internal medicine ,language ,Medicine ,Kazakh ,Esophageal cancer ,business ,medicine.disease ,Alcohol consumption ,language.human_language - Published
- 2007
37. Effect of shenfu injection on gastrointestinal microcirculation in rabbits after myocardial ischemia-reperfusion injury
- Author
-
Xiu-Mei Wang, Zan-Gong Zhou, Xue-Juan Zhang, and Li Song
- Subjects
Male ,Mean arterial pressure ,Hemodynamics ,Blood Pressure ,Myocardial Reperfusion Injury ,pCO2 ,Microcirculation ,Heart Rate ,Heart rate ,medicine ,Animals ,Gastrointestinal tract ,Dose-Response Relationship, Drug ,business.industry ,Gastroenterology ,General Medicine ,Hydrogen-Ion Concentration ,medicine.disease ,Gastrointestinal Tract ,Blood pressure ,Gastric Mucosa ,Anesthesia ,Injections, Intravenous ,Female ,Rabbits ,Blood Gas Analysis ,business ,Reperfusion injury ,Rapid Communication ,Drugs, Chinese Herbal - Abstract
AIM: To investigate the effect of shenfu injection on gastrointestinal microcirculation after myocardial ischemic-reperfusion (IR) injury in rabbits and probe into the mechanism. METHODS: Forty healthy flap-eared white rabbits were randomly divided into 4 groups: IR injury control group (group I), shenfu injection 5 mL/kg per h group (group II), shenfu injection 10 mL/kg per h group (group III) and shenfu injection 20 mL/kg per h group (group IV). The four groups were treated with Lactated Ringer’s solution, shenfu injection 5, 10, and 20 mL/ kg per h were infused intravenously 30 min before experiment respectively. The values of hemodynamics [mean arterial pressure (MAP), heart rate (HR), gastric intramucosal partial pressure of carbon dioxide (PCO2), blood gas analysis and pH] were measured and compared with those before myocardial ischemia, 60 min after myocardial ischemia and 60, 90, and 180 min after reperfusion. RESULTS: The MAP, HR and gastric intramucosal pH were (70.50 ± 4.50) kPa, (165 ± 14) beats per min, 7.032 ± 0.024 in groupI 60 min after myocardial ischemia, which were significantly decreased compared with those before myocardial ischemia (88.50 ± 9.75 kPa, 217 ± 18 beats per min, 7.112 ± 0.035, P < 0.05). The MAP, HR and gastric intramucosal pH were significantly decreased in groupI 60, 90, and 180 min after reperfusion (61.50 ± 5.25 kPa, 133 ± 31 beats per min, 6.997 ± 0.025) compared with those before reperfusion respectively (P < 0.05), whereas the values were insignificantly different in groups II, III or IV after reperfusion, compared with those before reperfusion, and there were no significant differences between groups II, III, and IV after reperfusion. CONCLUSION: Pre-infusion of shenfu injection has a protective effect on gastrointestinal microcirculation after myocardial IR injury in rabbits, in a dose independent manner.
- Published
- 2006
38. Activation of the ERK1/2 Signaling Pathway during the Osteogenic Differentiation of Mesenchymal Stem Cells Cultured on Substrates Modified with Various Chemical Groups.
- Author
-
Bing Bai, Jin He, Yan-Shu Li, Xiu-Mei Wang, Hong-Jun Ai, and Fu-Zhai Cui
- Abstract
The current study examined the influence of culture substrates modified with the functional groups -OH, -COOH, -NH
2 , and -CH3 using SAMs technology, in conjunction with TAAB control, on the osteogenic differentiation of rabbit BMSCs. The CCK-8 assay revealed that BMSCs exhibited substrate-dependent cell viability. The cells plated on -NH2 - and -OH-modified substrates were well spread and homogeneous, but those on the -COOH- and -CH3 -modified substrates showed more rounded phenotype. The mRNA expression of BMSCs revealed that -NH2 -modified substrate promoted the mRNA expression and osteogenic differentiation of the BMSCs. The contribution of ERK1/2 signaling pathway to the osteogenic differentiation of BMSCs cultured on the -NH2 -modified substrate was investigated in vitro. The -NH2 -modified substrate promoted the expression of integrins; the activation of FAK and ERK1/2. Inhibition of ERK1/2 activation by PD98059, a specific inhibitor of the ERK signaling pathway, blocked ERK1/2 activation in a dose-dependent manner, as revealed for expression of Cbfα-1 and ALP. Blockade of ERK1/2 phosphorylation in BMSCs by PD98059 suppressed osteogenic differentiation on chemical surfaces. These findings indicate a potential role for ERK in the osteogenic differentiation of BMSCs on surfaces modified by specific chemical functional groups, indicating that the microenvironment affects the differentiation of BMSCs. This observation has important implications for bone tissue engineering. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
39. The mechanical anisotropy on a longitudinal section of human enamel studied by nanoindentation.
- Author
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Zhen-jiang Cheng, Xiu-mei Wang, Jun Ge, Jian-xin Yan, Ning Ji, Li-li Tian, and Fu-zhai Cui
- Subjects
ANISOTROPY ,INDENTATION (Materials science) ,DENTAL enamel ,TEETH ,HYDROXYAPATITE - Abstract
Nanoindentation has been widely used for probing the mechanical properties of tooth, especially for characterizing its complex hierarchical structures. Previous studies have confirmed the anisotropic mechanical behaviors caused by the alternated orientations of enamel rods and the alignment of fibril-like hydroxyapatite crystals, but the longitudinal section of enamel, which was composed of parallel-arranged rods, was regarded as a homogeneous continuum as always. In this study, nanoindentation combined with SEM was carried out with the indenter rotating on the longitudinal section of enamel to evaluate the relativity between the nano-mechanical properties and the orientation of indentation impressions. It has been shown that the enamel presented different elastic modulus and hardness with different angles of indenter on its longitudinal section, and its anisotropy was also confirmed by the remarkable asymmetric morphologies of impressions. We observed that the parallel arrangement of crystal fibrils and enamel rods might trigger the expansion of the micro-cracks in preferred orientation, and result in scalene triangle indentation impressions, altering contact areas as well as inconsistent mechanical behaviors. Consequently, it is considered that the longitudinal sections of enamel should be modeled as anisotropic. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
40. Electronmicroscopic studies on the location of salmonella proliferation in the murine spleen
- Author
-
H. S. Hsu, V. R. Mumaw, Xiu-Mei Wang, Irene Nakoneczna, and F. R. Lin
- Subjects
Microbiology (medical) ,Male ,Salmonella typhimurium ,Salmonella ,Neutrophils ,Virulence ,Spleen ,Biology ,medicine.disease_cause ,Microbiology ,Mice ,medicine ,Extracellular ,Animals ,Murine spleen ,Salmonella Infections, Animal ,Macrophages ,General Medicine ,biology.organism_classification ,medicine.disease ,Mice, Inbred C57BL ,Microscopy, Electron ,medicine.anatomical_structure ,Tissue sections ,Infiltration (medical) ,Bacteria - Abstract
Summary Highly susceptible inbred male C57BL/6 mice were infected intraperitoneally with 2 × 107 cfu of a virulent Salmonella typhimurium strain. Tissue sections were taken from the spleen 2 and 3 days after infection for examination by electronmicroscopy. Rapid infiltration of polymorphs and macrophages was evident at the site of infection. These inflammatory phagocytes displayed avid destructive action against ingested bacteria. Bacterial multiplication occurred primarily in extracellular locations within sinusoids or in lesions containing disintegrating host cells.
- Published
- 1988
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