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目的 研究铁缺乏症患者缺铁程度对口服铁剂吸收的影响。 方法 纳入2018年7月至2020年6月于中国医学科学院血液病医院贫血中心门诊就诊并确诊铁缺乏症的非妊娠期女性患者37例，以13例健康查体女性为正常对照，分析缺铁性贫血（IDA）、缺铁性红细胞生成/储存铁不足（IDE/ID）患者及正常对照者Hepcidin水平并进行口服铁剂吸收试验（OIAT），比较服铁后2 h血清铁（C2）与基线血清铁（C0）差值。 结果 IDA、ID/IDE、正常对照组Hepcidin中位数分别为4.9（2.17～32.86）、26.98（11.02～49.71）、69.89（42.23～138.96）µg/L（P

Anthracnose is one of the most common diseases in strawberry plants. Colletotrichum gloeosporioides is the major cause of anthracnose in China, including Zhejiang Province. Early, specific, reliable, and time-saving detection is urgently needed to prevent the further spread of C. gloeosporioides, guiding farmers to utilize chemicals to control anthracnose. In this study, we showed that the high resistance to pyraclostrobin, caused by a point mutation at codon 143 (GGT→GCT) in the cytochrome b gene of C. gloeosporioides was prevalent in the strawberry growing regions, and we developed a loop-mediated isothermal amplification (LAMP) assay as a detection method. Primer sets S0 and S4 could be used to specifically detect C. gloeosporioides isolates and the G143A mutations, respectively. A detection limit of 10−2 ng (10 pg), which is at least 10-fold more sensitive than conventional polymerase chain reaction, was achieved by the LAMP assay. Here, we utilized lateral-flow devices (LFDs), nitrocellulose membranes that can absorb nucleic acids, to acquire the total genomic DNA of strawberry plants within 2 min. The LFD membranes were used as DNA templates for the LAMP assays to accurately detect strawberry plants infected with C. gloeosporioides. This diagnostic method for strawberry anthracnose was accomplished within 1 h, including the sample preparation and LAMP assays. Collectively, we developed a sensitive and practical method for monitoring C. gloeosporioides and its quinone outside inhibitor–resistant mutants. The LAMP assay for detection of C. gloeosporioides in strawberry plants has great potential for rapid strawberry anthracnose surveillance and will provide farmers with advice on preventing C gloeosporioides at the early stages of strawberry development.

目的 研究Erythroferrone（ERFE）等铁代谢红系调节因子（iron-regulatory erythroid factor）在不同类型红系造血异常疾病中的表达情况。 方法 采用ELISA方法检测2016年1月至2019年11月共47例真性红细胞增多症（PV）、纯红细胞再生障碍（PRCA）、自身免疫性溶血性贫血（AIHA）和骨髓增生异常综合征（MDS）患者血浆ERFE、生长分化因子15（GDF15）、生长分化因子11（GDF11）和扭转原肠胚形成同系物（TWSG1）的表达，分析铁代谢调节因子与红系造血异常类型及旺盛程度（以骨髓有核红细胞比例反映）的适配性。 结果 血浆GDF15表达水平在PV、PRCA、AIHA、MDS各组依次为266.01（112.40,452.37）、110.63（81.41,220.42）、52.11（32.61,171.66）、276.53（132.16,525.70）ng/L，均显著高于正常对照组的37.45（19.65,57.72）ng/L（P值均0.05）。血浆GDF11表达水平仅在PV组患者中明显高于正常对照组[74.75（10.95,121.32）ng/L对36.90（3.38,98.34）ng/L，P0.05）。PV组血浆ERFE水平为129.63（47.02, 170.03）ng/L，AIHA组血浆ERFE水平最高为121.76（68.12,343.11）ng/L，二者均明显高于正常对照组的43.23（35.18,65.41）ng/L（P值均0.05）。骨髓有核红细胞比例与ERFE（r＝0.458，P＝0.001）呈正相关，而与GDF15（r＝−0.163，P＝0.274）、GDF11（r＝0.120，P＝0.421）、TWSG1（r＝−0.166，P＝0.269）无明显相关性。 结论 铁代谢红系调节因子在不同红系造血异常疾病的表达谱不尽一致，ERFE与红系造血旺盛程度相关度最高。

Anthracnose is one of the most common diseases in strawberry plants.

The effect of natural killer (NK) cell alloreactivity on the outcome of unrelated hematopoietic SCT (HSCT) remains a topic of debate. NK cell alloreactivity after allogeneic HSCT is regulated by killer-cell Ig-like receptors (KIRs). To investigate the influence of KIRs on outcome after unrelated HSCT, we retrospectively analyzed the HLA and KIR genotypes of 116 donor-recipient pairs. We found that missing KIR ligands in recipients were significantly associated with a decreased leukemic relapse risk (P=0.019, HR=0.329), mainly in myeloid disease (P=0.003, HR=0.193). This beneficial effect was seen in AML/myelodysplastic syndrome and also in chronic myeloid leukemia. In myeloid disease, missing KIR ligands also improved 5-year OS (P=0.034, HR=0.430) and disease-free survival (DFS) (P=0.024, HR=0.445). Meanwhile, the presence of donor-activating KIR2DS3 gene was associated with increased relapse risk (P=0.003, HR=5.046), decreased OS (P=0.004, HR=3.181) and DFS (P=0.003, HR=2.919) in myeloid disease. No effect was seen in patients with lymphoid disease. Our study indicated that, in unrelated HSCT for myeloid leukemia, missing KIR ligands in recipients offered a lower relapse risk and a long-term survival advantage. The presence of KIR2DS3 in the donor was an important risk factor for myeloid leukemia.

C17H19CIN2, orthorhombic, P2i2i2i (No. 19), a = 18.0920(1)Â, b = 5.8896(1) A, c = 14.6011(2) A, V= 1555.8 A, Z = 4, Rgt(F) = 0.048, wRKi(F) = 0.114, T= 298 K. Source of material The mixture of p-methylbenzene sulfonyl chloride and dithiolamine was refluxed with stirring for 1 hour, then adding SO2CI2, refluxing for 6 hours. The reaction mixture was treated with l-[(4-chlorophenyl)(phenyl)]methylamine in diisopropylethylamine and refluxed for 6 hours. The mixture was dissolving in HBr-AcOH 30:70 (v/v) to obtained the title compound crystallized from n-BuOH as colorless needles. Experimental details The H atoms were localized from differential Fourier maps and included in the final cycles of refinement in riding model (d(C—H) = 0.95 A 1.04 Â). Discussion A great deal of attention has been devoted to the medicine with chiral center, since they usually have special biological activity. Consequently, much interest has been focused on obtaining compounds with such a feature. The title compound is a chiral intermediate used for the synthesis of cetirizine hydrochloride. It consists of three different rings,two phenyl rings and a piperazine ring. The dihedral angle between two phenyl rings is 116.78(6)°. Futhermore, piperazine ring is a chair-like conformation, this can be seen from the distance of N1 and N2 to the plane of C2-C3-C4-C5 (-0.699 A and 0.597 A, respectively). In addition, through Van der Waals interation, molecules pack into infinite V-type chains, which are oriented along «-axis and c-axis. Table 1. Data collection and handling. Crystal: colorless chunk, size 0.10 χ 0.11 χ 0.29 mm Wavelength: Mo Ka radiation (0.7107 Â) μ: 2.37 cnT Diffractometer, scan mode: Rigaku RAXIS-RAPID, ω 20max: 54.96° N(hkl)measured. N(hkl)unique: 14633,3565 Criterion for 70bs, N(hkl)gt: /obs>2a(/obs), 2251 N(param) refined: 200 Programs: SIR92 [3], ABSCOR [4], CrystalStructure [5] ° Ί Table 2. Atomic coordinates and displacement parameters (in A").

C17H19ClN2, orthorhombic, P212121 (No. 19), a = 18.0920(1) Å, b = 5.8896(1) Å, c = 14.6011(2) Å, V = 1555.8 Å3, Z = 4, Rgt(F) = 0.048, wRref(F2) = 0.114, T = 298 K.