1. Modification of Canadian Food and Drug Directorate Lipoxidase Method for Polyunsaturated Fatty Acid Determination
- Author
-
A. R. Prosser, Willard D. Hubbard, and Alan J. Sheppard
- Subjects
Drug ,chemistry.chemical_classification ,Chemistry ,media_common.quotation_subject ,General Chemistry ,Food science ,media_common ,Polyunsaturated fatty acid - Abstract
The Canadian FA-59 enzymatic method for determining cis,cis-methylene interrupted polyunsaturated fatty acids has been modified to meet the needs of the cholesterol and fatty acid regulations of the U.S. Food and Drug Administration. A centrifugation step has been introduced to remove palmitate and stearate interference with absorbance readings for samples containing low amounts of cis,cis-methylene interrupted polyunsaturated fatty acids. The data are calculated by using a standard curve and an equation which yields g trilinolein/100 g product rather than % linoleic acid in a given weight of sample. The standard curve was linear for 1–12 μg trilinolein. Recovery of added trilinolein was 99.5%, with 4% coefficient of variation. The modified procedure was successfully applied to a wide variety of foodstuffs.
- Published
- 1977