25 results on '"Wiesenberger G"'
Search Results
2. Analysis of the Saccharomyces cerevisiae mitochondrial COX3 mRNA 5' untranslated leader: translational activation and mRNA processing
- Author
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Wiesenberger, G, primary, Costanzo, M C, additional, and Fox, T D, additional
- Published
- 1995
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3. The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo.
- Author
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Wiesenberger, G, primary, Waldherr, M, additional, and Schweyen, R.J., additional
- Published
- 1992
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4. Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants.
- Author
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Vogel, Jörg, Börner, Thomas, Hess, Wolfgang R., Michel, F., Kim, J.K., Matsuura, M., Holländer, V., Vogel, J., Podar, M., Wiesenberger, G., Waldherr, M., Jenkins, B.D., Hess, W.R., Hübschmann, T., Oda, K., Hagemann, R., Rogers, S., Sexton, T., Boyer, S., and Kössel, H.
- Published
- 1999
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5. Mechanism of Fumonisin Self-Resistance: Fusarium verticillioides Contains Four Fumonisin B 1 -Insensitive-Ceramide Synthases.
- Author
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Krska T, Twaruschek K, Wiesenberger G, Berthiller F, and Adam G
- Subjects
- Drug Resistance, Fungal genetics, Fungal Proteins genetics, Fungal Proteins metabolism, Aspergillus genetics, Aspergillus metabolism, Aspergillus enzymology, Alternaria genetics, Alternaria enzymology, Fumonisins metabolism, Fusarium genetics, Fusarium metabolism, Fusarium enzymology, Oxidoreductases metabolism, Oxidoreductases genetics
- Abstract
Fusarium verticillioides produces fumonisins, which are mycotoxins inhibiting sphingolipid biosynthesis in humans, animals, and other eukaryotes. Fumonisins are presumed virulence factors of plant pathogens, but may also play a role in interactions between competing fungi. We observed higher resistance to added fumonisin B
1 (FB1 ) in fumonisin-producing Fusarium verticillioides than in nonproducing F. graminearum , and likewise between isolates of Aspergillus and Alternaria differing in production of sphinganine-analog toxins. It has been reported that in F. verticillioides , ceramide synthase encoded in the fumonisin biosynthetic gene cluster is responsible for self-resistance. We reinvestigated the role of FUM17 and FUM18 by generating a double mutant strain in a fum1 background. Nearly unchanged resistance to added FB1 was observed compared to the parental fum1 strain. A recently developed fumonisin-sensitive baker's yeast strain allowed for the testing of candidate ceramide synthases by heterologous expression. The overexpression of the yeast LAC1 gene, but not LAG1 , increased fumonisin resistance. High-level resistance was conferred by FUM18 , but not by FUM17 . Likewise, strong resistance to FB1 was caused by overexpression of the presumed F. verticillioides "housekeeping" ceramide synthases CER1 , CER2 , and CER3 , located outside the fumonisin cluster, indicating that F. verticillioides possesses a redundant set of insensitive targets as a self-resistance mechanism.- Published
- 2024
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6. Development of a fumonisin-sensitive Saccharomyces cerevisiae indicator strain and utilization for activity testing of candidate detoxification genes.
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Krska T, Twaruschek K, Valente N, Mitterbauer R, Moll D, Wiesenberger G, Berthiller F, and Adam G
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- Humans, Animals, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Animal Feed, Fumonisins toxicity, Fumonisins metabolism, Fusarium genetics, Fusarium metabolism
- Abstract
Importance: Fumonisins can cause diseases in animals and humans consuming Fusarium -contaminated food or feed. The search for microbes capable of fumonisin degradation, or for enzymes that can detoxify fumonisins, currently relies primarily on chemical detection methods. Our constructed fumonisin B1-sensitive yeast strain can be used to phenotypically detect detoxification activity and should be useful in screening for novel fumonisin resistance genes and to elucidate fumonisin metabolism and resistance mechanisms in fungi and plants, and thereby, in the long term, help to mitigate the threat of fumonisins in feed and food., Competing Interests: There is a conflict of interest due to T. Krska and K. Twaruschek being employed by FFoQSI. The initial stages of this work have also been funded by Biomin Holding GmbH (part of dsm-firmenich, employing Wulf-Dieter Moll) and the final stages by FFoQSI GmbH (Austrian Competence Centre for Feed and Food Quality, Safety & Innovation, project C30-P12-W03: Toxin Inactivation). IP rights have been transferred to FFoQSI, which filed a patent application (PCT/EP2023/062973).
- Published
- 2023
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7. Identification of a UDP-glucosyltransferase conferring deoxynivalenol resistance in Aegilops tauschii and wheat.
- Author
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Kirana RP, Gaurav K, Arora S, Wiesenberger G, Doppler M, Michel S, Zimmerl S, Matic M, Eze CE, Kumar M, Topuz A, Lemmens M, Schuhmacher R, Adam G, Wulff BBH, Buerstmayr H, and Steiner B
- Subjects
- Triticum genetics, Triticum metabolism, Glucosyltransferases genetics, Uridine Diphosphate, Plant Breeding, Plant Diseases genetics, Disease Resistance genetics, Aegilops, Fusarium
- Abstract
Aegilops tauschii is the diploid progenitor of the wheat D subgenome and a valuable resource for wheat breeding, yet, genetic analysis of resistance against Fusarium head blight (FHB) and the major Fusarium mycotoxin deoxynivalenol (DON) is lacking. We treated a panel of 147 Ae. tauschii accessions with either Fusarium graminearum spores or DON solution and recorded the associated disease spread or toxin-induced bleaching. A k-mer-based association mapping pipeline dissected the genetic basis of resistance and identified candidate genes. After DON infiltration nine accessions revealed severe bleaching symptoms concomitant with lower conversion rates of DON into the non-toxic DON-3-O-glucoside. We identified the gene AET5Gv20385300 on chromosome 5D encoding a uridine diphosphate (UDP)-glucosyltransferase (UGT) as the causal variant and the mutant allele resulting in a truncated protein was only found in the nine susceptible accessions. This UGT is also polymorphic in hexaploid wheat and when expressed in Saccharomyces cerevisiae only the full-length gene conferred resistance against DON. Analysing the D subgenome helped to elucidate the genetic control of FHB resistance and identified a UGT involved in DON detoxification in Ae. tauschii and hexaploid wheat. This resistance mechanism is highly conserved since the UGT is orthologous to the barley UGT HvUGT13248 indicating descent from a common ancestor of wheat and barley., (© 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
- Published
- 2023
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8. Gramiketides, Novel Polyketide Derivatives of Fusarium graminearum , Are Produced during the Infection of Wheat.
- Author
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Seidl B, Rehak K, Bueschl C, Parich A, Buathong R, Wolf B, Doppler M, Mitterbauer R, Adam G, Khewkhom N, Wiesenberger G, and Schuhmacher R
- Abstract
The plant pathogen Fusarium graminearum is a proficient producer of mycotoxins and other in part still unknown secondary metabolites, some of which might act as virulence factors on wheat. The PKS15 gene is expressed only in planta , so far hampering the identification of an associated metabolite. Here we combined the activation of silent gene clusters by chromatin manipulation ( kmt6 ) with blocking the metabolic flow into the competing biosynthesis of the two major mycotoxins deoxynivalenol and zearalenone. Using an untargeted metabolomics approach, two closely related metabolites were found in triple mutants ( kmt6 tri5 pks4,13) deficient in production of the major mycotoxins deoxynivalenol and zearalenone, but not in strains with an additional deletion in PKS15 (kmt6 tri5 pks4,13 pks15) . Characterization of the metabolites, by LC-HRMS/MS in combination with a stable isotope-assisted tracer approach, revealed that they are likely hybrid polyketides comprising a polyketide part consisting of malonate-derived acetate units and a structurally deviating part. We propose the names gramiketide A and B for the two metabolites. In a biological experiment, both gramiketides were formed during infection of wheat ears with wild-type but not with pks15 mutants. The formation of the two gramiketides during infection correlated with that of the well-known virulence factor deoxynivalenol, suggesting that they might play a role in virulence.
- Published
- 2022
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9. Identification and Functional Characterisation of Two Oat UDP-Glucosyltransferases Involved in Deoxynivalenol Detoxification.
- Author
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Khairullina A, Tsardakas Renhuldt N, Wiesenberger G, Bentzer J, Collinge DB, Adam G, and Bülow L
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- Avena metabolism, Glucosyltransferases genetics, Glucosyltransferases metabolism, Plant Proteins metabolism, Trichothecenes, Uridine Diphosphate metabolism, Fusarium metabolism, Mycotoxins metabolism
- Abstract
Oat is susceptible to several Fusarium species that cause contamination with different trichothecene mycotoxins. The molecular mechanisms behind Fusarium resistance in oat have yet to be elucidated. In the present work, we identified and characterised two oat UDP-glucosyltransferases orthologous to barley HvUGT13248. Overexpression of the latter in wheat had been shown previously to increase resistance to deoxynivalenol (DON) and nivalenol (NIV) and to decrease disease the severity of both Fusarium head blight and Fusarium crown rot. Both oat genes are highly inducible by the application of DON and during infection with Fusarium graminearum . Heterologous expression of these genes in a toxin-sensitive strain of Saccharomyces cerevisiae conferred high levels of resistance to DON, NIV and HT-2 toxins, but not C4-acetylated trichothecenes (T-2, diacetoxyscirpenol). Recombinant enzymes AsUGT1 and AsUGT2 expressed in Escherichia coli rapidly lost activity upon purification, but the treatment of whole cells with the toxin clearly demonstrated the ability to convert DON into DON-3-O-glucoside. The two UGTs could therefore play an important role in counteracting the Fusarium virulence factor DON in oat.
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- 2022
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10. Zearalenone and ß-Zearalenol But Not Their Glucosides Inhibit Heat Shock Protein 90 ATPase Activity.
- Author
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Torres Acosta JA, Michlmayr H, Shams M, Schweiger W, Wiesenberger G, Mitterbauer R, Werner U, Merz D, Hauser MT, Hametner C, Varga E, Krska R, Berthiller F, and Adam G
- Abstract
The mycotoxin zearalenone (ZEN) is produced by many plant pathogenic Fusarium species. It is well known for its estrogenic activity in humans and animals, but whether ZEN has a role in plant-pathogen interaction and which process it is targeting in planta was so far unclear. We found that treatment of Arabidopsis thaliana seedlings with ZEN induced transcription of the AtHSP90.1 gene. This heat shock protein (HSP) plays an important role in plant-pathogen interaction, assisting in stability and functionality of various disease resistance gene products. Inhibition of HSP90 ATPase activity impairs functionality. Because HSP90 inhibitors are known to induce HSP90 gene expression and due to the structural similarity with the known HSP90 inhibitor radicicol (RAD), we tested whether ZEN and its phase I metabolites α- and ß-zearalenol are also HSP90 ATPase inhibitors. Indeed, At HSP90.1 and wheat Ta HSP90-2 were inhibited by ZEN and ß-zearalenol, while α-zearalenol was almost inactive. Plants can efficiently glycosylate ZEN and α/ß-zearalenol. We therefore tested whether glucosylation has an effect on the inhibitory activity of these metabolites. Expression of the A. thaliana glucosyltransferase UGT73C6 conferred RAD resistance to a sensitive yeast strain. Glucosylation of RAD, ZEN, and α/ß-zearalenol abolished the in vitro inhibitory activity with recombinant HSP90 purified from Escherichia coli . In conclusion, the mycotoxin ZEN has a very prominent target in plants, HSP90, but it can be inactivated by glycosylation. This may explain why there is little evidence for a virulence function of ZEN in host plants., (Copyright © 2019 Torres Acosta, Michlmayr, Shams, Schweiger, Wiesenberger, Mitterbauer, Werner, Merz, Hauser, Hametner, Varga, Krska, Berthiller and Adam.)
- Published
- 2019
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11. Biochemical Characterization of the Fusarium graminearum Candidate ACC-Deaminases and Virulence Testing of Knockout Mutant Strains.
- Author
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Svoboda T, Parich A, Güldener U, Schöfbeck D, Twaruschek K, Václavíková M, Hellinger R, Wiesenberger G, Schuhmacher R, and Adam G
- Abstract
Fusarium graminearum is a plant pathogenic fungus which is able to infect wheat and other economically important cereal crop species. The role of ethylene in the interaction with host plants is unclear and controversial. We have analyzed the inventory of genes with a putative function in ethylene production or degradation of the ethylene precursor 1-aminocyclopropane carboxylic acid (ACC). F. graminearum , in contrast to other species, does not contain a candidate gene encoding ethylene-forming enzyme. Three genes with similarity to ACC synthases exist; heterologous expression of these did not reveal enzymatic activity. The F. graminearum genome contains in addition two ACC deaminase candidate genes. We have expressed both genes in E. coli and characterized the enzymatic properties of the affinity-purified products. One of the proteins had indeed ACC deaminase activity, with kinetic properties similar to ethylene-stress reducing enzymes of plant growth promoting bacteria. The other candidate was inactive with ACC but turned out to be a d-cysteine desulfhydrase. Since it had been reported that ethylene insensitivity in transgenic wheat increased Fusarium resistance and reduced the content of the mycotoxin deoxynivalenol (DON) in infected wheat, we generated single and double knockout mutants of both genes in the F. graminearum strain PH-1. No statistically significant effect of the gene disruptions on fungal spread or mycotoxin content was detected, indicating that the ability of the fungus to manipulate the production of the gaseous plant hormones ethylene and H
2 S is dispensable for full virulence.- Published
- 2019
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12. New Plasmids for Fusarium Transformation Allowing Positive-Negative Selection and Efficient Cre- loxP Mediated Marker Recycling.
- Author
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Twaruschek K, Spörhase P, Michlmayr H, Wiesenberger G, and Adam G
- Abstract
In filamentous fungi such as Fusarium graminearum , disruption of multiple genes of interest in the same strain (e.g., to test for redundant gene function) is a difficult task due to the limited availability of reliable selection markers. We have created a series of transformation vectors that allow antibiotic-based selection of transformants and subsequent negative selection for marker removal using thymidine kinase fusions combined with the Cre -loxP system. The fusion genes contain commonly used C-terminal drug resistance markers, either nptII (G418), nat1 (nourseothricin), or hph (hygromycin B). These resistance genes are fused to the sequence encoding Herpes simplex virus thymidine kinase (HSVtk). Despite the presence of the 1 kb HSVtk gene (about ∼30% increase in total marker size), there is only a slight reduction in transformation efficiency on a molar basis. The fusion genes expressed under the Trichoderma pyruvate kinase (PKI) promoter also confer antibiotic resistance in Escherichia coli , allowing straightforward construction of disruption plasmids. For removal of the loxP flanked resistance cassettes, protoplasts of transformants are directly treated with purified Cre recombinase protein. Loss of the HSVtk containing cassette is selected by restoration of resistance to 5-fluoro-2-deoxyuridine (FdU). As a proof of principle, we demonstrated the efficiency of the HSVtk-based marker removal in Fusarium by reversing the disruption phenotype of the gene responsible for production of the red pigment aurofusarin. We first disrupted the FgPKS12 gene via integration of the loxP -flanked HSVtk -nptII cassette into the promoter or the first intron, thereby generating transformants with a white mycelium phenotype. Using Cre recombinase and FdU, the selection marker was subsequently removed, and the resulting transformants regained red pigmentation despite the remaining loxP site. We also found that it is possible to remove several unselected loxP -flanked cassettes with a single Cre protein treatment, as long as one of them contains a negative selectable HSVtk cassette. The negative selection system can also be used to introduce allele swaps into strains without leaving marker sequences, by first disrupting the gene of interest and then complementing the deletion in situ with genomic DNA containing a different allele.
- Published
- 2018
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13. A barley UDP-glucosyltransferase inactivates nivalenol and provides Fusarium Head Blight resistance in transgenic wheat.
- Author
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Li X, Michlmayr H, Schweiger W, Malachova A, Shin S, Huang Y, Dong Y, Wiesenberger G, McCormick S, Lemmens M, Fruhmann P, Hametner C, Berthiller F, Adam G, and Muehlbauer GJ
- Subjects
- Disease Resistance genetics, Glucosyltransferases metabolism, Hordeum enzymology, Hordeum microbiology, Host-Pathogen Interactions, Plant Diseases microbiology, Plant Proteins metabolism, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, Plants, Genetically Modified microbiology, Triticum genetics, Triticum metabolism, Triticum microbiology, Fusarium metabolism, Glucosyltransferases genetics, Hordeum genetics, Plant Diseases genetics, Plant Proteins genetics, Trichothecenes metabolism
- Abstract
Fusarium Head Blight is a disease of cereal crops that causes severe yield losses and mycotoxin contamination of grain. The main causal pathogen, Fusarium graminearum, produces the trichothecene toxins deoxynivalenol or nivalenol as virulence factors. Nivalenol-producing isolates are most prevalent in Asia but co-exist with deoxynivalenol producers in lower frequency in North America and Europe. Previous studies identified a barley UDP-glucosyltransferase, HvUGT13248, that efficiently detoxifies deoxynivalenol, and when expressed in transgenic wheat results in high levels of type II resistance against deoxynivalenol-producing F. graminearum. Here we show that HvUGT13248 is also capable of converting nivalenol into the non-toxic nivalenol-3-O-β-d-glucoside. We describe the enzymatic preparation of a nivalenol-glucoside standard and its use in development of an analytical method to detect the nivalenol-glucoside conjugate. Recombinant Escherichia coli expressing HvUGT13248 glycosylates nivalenol more efficiently than deoxynivalenol. Overexpression in yeast, Arabidopsis thaliana, and wheat leads to increased nivalenol resistance. Increased ability to convert nivalenol to nivalenol-glucoside was observed in transgenic wheat, which also exhibits type II resistance to a nivalenol-producing F. graminearum strain. Our results demonstrate the HvUGT13248 can act to detoxify deoxynivalenol and nivalenol and provide resistance to deoxynivalenol- and nivalenol-producing Fusarium., (© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Published
- 2017
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14. Pentahydroxyscirpene-Producing Strains, Formation In Planta, and Natural Occurrence.
- Author
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Varga E, Wiesenberger G, Fruhmann P, Malachová A, Svoboda T, Lemmens M, Adam G, and Berthiller F
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- DNA, Fungal analysis, Food Contamination analysis, Fusarium genetics, Hordeum chemistry, Hordeum microbiology, Oryza chemistry, Ribosomes drug effects, Trichothecenes toxicity, Triticum chemistry, Fusarium metabolism, Oryza microbiology, Trichothecenes metabolism, Triticum microbiology
- Abstract
Trichothecenes are a class of structurally diverse mycotoxins with more than 200 naturally occurring compounds. Previously, a new compound, pentahydroxyscirpene (PHS), was reported as a byproduct of a nivalenol producing Fusarium strain, IFA189. PHS contains a hydroxy group at C-8 instead of the keto group of type B trichothecenes. In this work, we demonstrate that IFA189 belongs to the species Fusarium kyushuense using molecular tools. Production of PHS in vitro was also observed for several isolates of other Fusarium species producing nivalenol. Furthermore, we report the formation of 4-acetyl-PHS by F. kyushuense on inoculated rice. Wheat ears of the variety Remus were infected with IFA189 and the in planta production of PHS was confirmed. Natural occurrence of PHS was verified in barley samples from the Czech Republic using a liquid chromatographic-tandem mass spectrometric method validated for this purpose. Toxicity of PHS to wheat ribosomes was evaluated with a coupled in vitro transcription and translation assay, which showed that PHS inhibits protein biosynthesis slightly less than nivalenol and deoxynivalenol., Competing Interests: The authors declare no conflict of interest.
- Published
- 2016
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15. Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells.
- Author
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Warth B, Del Favero G, Wiesenberger G, Puntscher H, Woelflingseder L, Fruhmann P, Sarkanj B, Krska R, Schuhmacher R, Adam G, and Marko D
- Abstract
The mycotoxin deoxynivalenol (DON) is an abundant contaminant of cereal based food and a severe issue for global food safety. We report the discovery of DON-3-sulfate as a novel human metabolite and potential new biomarker of DON exposure. The conjugate was detectable in 70% of urine samples obtained from pregnant women in Croatia. For the measurement of urinary metabolites, a highly sensitive and selective LC-MS/MS method was developed and validated. The method was also used to investigate samples from a duplicate diet survey for studying the toxicokinetics of DON-3-sulfate. To get a preliminary insight into the biological relevance of the newly discovered DON-sulfates, in vitroexperiments were performed. In contrast to DON, sulfate conjugates lacked potency to suppress protein translation. However, surprisingly we found that DON-sulfates enhanced proliferation of human HT-29 colon carcinoma cells, primary human colon epithelial cells (HCEC-1CT) and, to some extent, also T24 bladder cancer cells. A proliferative stimulus, especially in tumorigenic cells raises concern on the potential impact of DON-sulfates on consumer health. Thus, a further characterization of their toxicological relevance should be of high priority.
- Published
- 2016
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16. Identification and Characterization of Carboxylesterases from Brachypodium distachyon Deacetylating Trichothecene Mycotoxins.
- Author
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Schmeitzl C, Varga E, Warth B, Kugler KG, Malachová A, Michlmayr H, Wiesenberger G, Mayer KF, Mewes HW, Krska R, Schuhmacher R, Berthiller F, and Adam G
- Subjects
- Acetylation, Brachypodium enzymology, Genes, Plant, Saccharomyces cerevisiae genetics, Trichothecenes chemistry, Trichothecenes toxicity, Brachypodium genetics, Carboxylic Ester Hydrolases genetics, Carboxylic Ester Hydrolases metabolism, Plant Proteins genetics, Plant Proteins metabolism, Trichothecenes metabolism
- Abstract
Increasing frequencies of 3-acetyl-deoxynivalenol (3-ADON)-producing strains of Fusarium graminearum (3-ADON chemotype) have been reported in North America and Asia. 3-ADON is nearly nontoxic at the level of the ribosomal target and has to be deacetylated to cause inhibition of protein biosynthesis. Plant cells can efficiently remove the acetyl groups of 3-ADON, but the underlying genes are yet unknown. We therefore performed a study of the family of candidate carboxylesterases (CXE) genes of the monocot model plant Brachypodium distachyon. We report the identification and characterization of the first plant enzymes responsible for deacetylation of trichothecene toxins. The product of the BdCXE29 gene efficiently deacetylates T-2 toxin to HT-2 toxin, NX-2 to NX-3, both 3-ADON and 15-acetyl-deoxynivalenol (15-ADON) into deoxynivalenol and, to a lesser degree, also fusarenon X into nivalenol. The BdCXE52 esterase showed lower activity than BdCXE29 when expressed in yeast and accepts 3-ADON, NX-2, 15-ADON and, to a limited extent, fusarenon X as substrates. Expression of these Brachypodium genes in yeast increases the toxicity of 3-ADON, suggesting that highly similar genes existing in crop plants may act as susceptibility factors in Fusarium head blight disease.
- Published
- 2015
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17. Metabolism of deoxynivalenol and deepoxy-deoxynivalenol in broiler chickens, pullets, roosters and turkeys.
- Author
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Schwartz-Zimmermann HE, Fruhmann P, Dänicke S, Wiesenberger G, Caha S, Weber J, and Berthiller F
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- Animals, Biotransformation, Feces chemistry, Female, Jejunum chemistry, Jejunum metabolism, Magnetic Resonance Spectroscopy, Male, Mycotoxins chemical synthesis, Mycotoxins toxicity, Reproducibility of Results, Sulfates metabolism, Tissue Distribution, Trichothecenes chemical synthesis, Trichothecenes toxicity, Chickens metabolism, Mycotoxins pharmacokinetics, Trichothecenes pharmacokinetics, Turkeys metabolism
- Abstract
Recently, deoxynivalenol-3-sulfate (DON-3-sulfate) was proposed as a major DON metabolite in poultry. In the present work, the first LC-MS/MS based method for determination of DON-3-sulfate, deepoxy-DON-3-sulfate (DOM-3-sulfate), DON, DOM, DON sulfonates 1, 2, 3, and DOM sulfonate 2 in excreta samples of chickens and turkeys was developed and validated. To this end, DOM-3-sulfate was chemically synthesized and characterized by NMR and LC-HR-MS/MS measurements. Application of the method to excreta and chyme samples of four feeding trials with turkeys, chickens, pullets, and roosters confirmed DON-3-sulfate as the major DON metabolite in all poultry species studied. Analogously to DON-3-sulfate, DOM-3-sulfate was formed after oral administration of DOM both in turkeys and in chickens. In addition, pullets and roosters metabolized DON into DOM-3-sulfate. In vitro transcription/translation assays revealed DOM-3-sulfate to be 2000 times less toxic on the ribosome than DON. Biological recoveries of DON and DOM orally administered to broiler chickens, turkeys, and pullets were 74%-106% (chickens), 51%-72% (roosters), and 131%-151% (pullets). In pullets, DON-3-sulfate concentrations increased from jejunum chyme samples to excreta samples by a factor of 60. This result, put into context with earlier studies, indicates fast and efficient absorption of DON between crop and jejunum, conversion to DON-3-sulfate in intestinal mucosa, liver, and possibly kidney, and rapid elimination into excreta via bile and urine.
- Published
- 2015
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18. Deoxynivalenol-sulfates: identification and quantification of novel conjugated (masked) mycotoxins in wheat.
- Author
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Warth B, Fruhmann P, Wiesenberger G, Kluger B, Sarkanj B, Lemmens M, Hametner C, Fröhlich J, Adam G, Krska R, and Schuhmacher R
- Subjects
- Calibration, Chromatography, Liquid, Molecular Structure, Mycotoxins toxicity, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Trichothecenes toxicity, Triticum microbiology, Food Contamination analysis, Fusarium metabolism, Mycotoxins analysis, Trichothecenes analysis, Triticum chemistry
- Abstract
We report the identification of deoxynivalenol-3-sulfate and deoxynivalenol-15-sulfate as two novel metabolites of the trichothecene mycotoxin deoxynivalenol in wheat. Wheat ears which were either artificially infected with Fusarium graminearum or directly treated with the major Fusarium toxin deoxynivalenol (DON) were sampled 96 h after treatment. Reference standards, which have been chemically synthesized and confirmed by NMR, were used to establish a liquid chromatography-electrospray ionization (LC-ESI)-MS/MS-based "dilute and shoot" method for the detection, unambiguous identification, and quantification of both sulfate conjugates in wheat extracts. Using this approach, detection limits of 0.003 mg/kg for deoxynivalenol-3-sulfate and 0.002 mg/kg for deoxynivalenol-15-sulfate were achieved. Matrix-matched calibration was used for the quantification of DON-sulfates in the investigated samples. In DON-treated samples, DON-3-sulfate was detected in the range of 0.29-1.4 mg/kg fresh weight while DON-15-sulfate concentrations were significantly lower (range 0.015-0.061 mg/kg fresh weight). In Fusarium-infected wheat samples, DON-3-sulfate was the only detected sulfate conjugate (range 0.022-0.059 mg/kg fresh weight). These results clearly demonstrate the potential of wheat to form sulfate conjugates of DON. In order to test whether sulfation is a detoxification reaction in planta, we determined the ability of the sulfated DON derivatives to inhibit in vitro protein synthesis of wheat ribosomes. The results demonstrate that both DON-sulfates can be regarded as detoxification products. DON-15-sulfate was about 44× less inhibitory than the native toxin, and no toxicity was observed for DON-3-sulfate in the tested range.
- Published
- 2015
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19. Individual and combined roles of malonichrome, ferricrocin, and TAFC siderophores in Fusarium graminearum pathogenic and sexual development.
- Author
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Oide S, Berthiller F, Wiesenberger G, Adam G, and Turgeon BG
- Abstract
Intra- and extracellular iron-chelating siderophores produced by fungal non-ribosomal peptide synthetases have been shown to be involved in reproductive and pathogenic developmental processes and in iron and oxidative stress management. Here we report individual and combined contributions of three of these metabolites to developmental success of the destructive cereal pathogen Fusarium graminearum. In previous work, we determined that deletion of the NPS2 gene, responsible for intracellular siderophore biosynthesis, results in inability to produce sexual spores when mutants of this homothallic ascomycete are selfed. Deletion of the NPS6 gene, required for extracellular siderophore biosynthesis, does not affect sexual reproduction but results in sensitivity to iron starvation and oxidative stress and leads to reduced virulence to the host. Building on this, we report that double mutants lacking both NPS2 and NPS6 are augmented in all collective phenotypes of single deletion strains (i.e., abnormal sexual and pathogenic development, hypersensitivity to oxidative and iron-depletion stress), which suggests overlap of function. Using comparative biochemical analysis of wild-type and mutant strains, we show that NPS1, a third gene associated with siderophore biosynthesis, is responsible for biosynthesis of a second extracellular siderophore, malonichrome. nps1 mutants fail to produce this metabolite. Phenotypic characterization reveals that, although single nps1 mutants are like wild-type with respect to sexual development, hypersensitivity to ROS and iron-depletion stress, and virulence to the host, triple nps1nps2nps6 deletion strains, lacking all three siderophores, are even more impaired in these attributes than double nps2nps6 strains. Thus, combinatorial mutants lacking key iron-associated genes uncovered malonichrome function. The intimate connection between presence/absence of siderophores and resistance/sensitivity to ROS is central to sexual and pathogenic development.
- Published
- 2015
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20. Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa-5A, identifies novel candidate genes.
- Author
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Schweiger W, Steiner B, Ametz C, Siegwart G, Wiesenberger G, Berthiller F, Lemmens M, Jia H, Adam G, Muehlbauer GJ, Kreil DP, and Buerstmayr H
- Subjects
- Carrier Proteins metabolism, Disease Resistance immunology, Fusarium drug effects, Gene Expression Regulation, Plant drug effects, Genes, Plant genetics, Physical Chromosome Mapping, Plant Diseases genetics, Plant Diseases immunology, Plant Diseases virology, RNA, Messenger genetics, RNA, Messenger metabolism, Transcription, Genetic drug effects, Transcriptome drug effects, Trichothecenes pharmacology, Triticum immunology, Disease Resistance genetics, Fusarium physiology, Genetic Association Studies, Quantitative Trait Loci genetics, Transcriptome genetics, Triticum genetics, Triticum microbiology
- Abstract
Fusarium head blight, caused by Fusarium graminearum, is a devastating disease of wheat. We developed near-isogenic lines (NILs) differing in the two strongest known F. graminearum resistance quantitative trait loci (QTLs), Qfhs.ndsu-3BS (also known as resistance gene Fhb1) and Qfhs.ifa-5A, which are located on the short arm of chromosome 3B and on chromosome 5A, respectively. These NILs showing different levels of resistance were used to identify transcripts that are changed significantly in a QTL-specific manner in response to the pathogen and between mock-inoculated samples. After inoculation with F. graminearum spores, 16 transcripts showed a significantly different response for Fhb1 and 352 for Qfhs.ifa-5A. Notably, we identified a lipid transfer protein which is constitutively at least 50-fold more abundant in plants carrying the resistant allele of Qfhs.ifa-5A. In addition to this candidate gene associated with Qfhs.ifa-5A, we identified a uridine diphosphate (UDP)-glycosyltransferase gene, designated TaUGT12887, exhibiting a positive difference in response to the pathogen in lines harbouring both QTLs relative to lines carrying only the Qfhs.ifa-5A resistance allele, suggesting Fhb1 dependence of this transcript. Yet, this dependence was observed only in the NIL with already higher basal resistance. The complete cDNA of TaUGT12887 was reconstituted from available wheat genomic sequences, and a synthetic recoded gene was expressed in a toxin-sensitive strain of Saccharomyces cerevisiae. This gene conferred deoxynivalenol resistance, albeit much weaker than that observed with the previously characterized barley HvUGT13248., (2013 THE AUTHORS. MOLECULAR PLANT PATHOLOGY PUBLISHED BY JOHN WILEY & SONS LTD AND BSPP.)
- Published
- 2013
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21. Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes.
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Schweiger W, Pasquet JC, Nussbaumer T, Paris MP, Wiesenberger G, Macadré C, Ametz C, Berthiller F, Lemmens M, Saindrenan P, Mewes HW, Mayer KF, Dufresne M, and Adam G
- Subjects
- Amino Acid Sequence, Brachypodium genetics, Fusarium chemistry, Gene Dosage, Gene Expression Regulation, Plant, Gene Order, Glucosides metabolism, Glycosyltransferases genetics, Molecular Sequence Data, Multigene Family, Mutation, Mycotoxins genetics, Mycotoxins metabolism, Oryza enzymology, Oryza genetics, Phylogeny, Plant Proteins genetics, Plant Proteins metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Sorghum enzymology, Sorghum genetics, Species Specificity, Synteny, Brachypodium enzymology, Fusarium pathogenicity, Glycosyltransferases metabolism, Plant Diseases microbiology, Trichothecenes metabolism
- Abstract
Plant small-molecule UDP-glycosyltransferases (UGT) glycosylate a vast number of endogenous substances but also act in detoxification of metabolites produced by plant-pathogenic microorganisms. The ability to inactivate the Fusarium graminearum mycotoxin deoxynivalenol (DON) into DON-3-O-glucoside is crucial for resistance of cereals. We analyzed the UGT gene family of the monocot model species Brachypodium distachyon and functionally characterized two gene clusters containing putative orthologs of previously identified DON-detoxification genes from Arabidopsis thaliana and barley. Analysis of transcription showed that UGT encoded in both clusters are highly inducible by DON and expressed at much higher levels upon infection with a wild-type DON-producing F. graminearum strain compared with infection with a mutant deficient in DON production. Expression of these genes in a toxin-sensitive strain of Saccharomyces cerevisiae revealed that only two B. distachyon UGT encoded by members of a cluster of six genes homologous to the DON-inactivating barley HvUGT13248 were able to convert DON into DON-3-O-glucoside. Also, a single copy gene from Sorghum bicolor orthologous to this cluster and one of three putative orthologs of rice exhibit this ability. Seemingly, the UGT genes undergo rapid evolution and changes in copy number, making it difficult to identify orthologs with conserved substrate specificity.
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- 2013
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22. Yeast mitochondria import ATP through the calcium-dependent ATP-Mg/Pi carrier Sal1p, and are ATP consumers during aerobic growth in glucose.
- Author
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Traba J, Froschauer EM, Wiesenberger G, Satrústegui J, and Del Arco A
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- Aerobiosis, Biological Transport, Luciferases analysis, Luciferases genetics, Luciferases metabolism, Magnesium metabolism, Mitochondria enzymology, Mitochondria genetics, Mitochondrial ADP, ATP Translocases genetics, Phosphates metabolism, Phosphorylation, Saccharomyces cerevisiae enzymology, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Adenosine Triphosphate metabolism, Calcium metabolism, Glucose metabolism, Mitochondria metabolism, Mitochondrial ADP, ATP Translocases metabolism, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Sal1p, a novel Ca2+-dependent ATP-Mg/Pi carrier, is essential in yeast lacking all adenine nucleotide translocases. By targeting luciferase to the mitochondrial matrix to monitor mitochondrial ATP levels, we show in isolated mitochondria that both ATP-Mg and free ADP are taken up by Sal1p with a K(m) of 0.20 +/- 0.03 mM and 0.28 +/- 0.06 mM respectively. Nucleotide transport along Sal1p is strictly Ca2+ dependent. Ca2+ increases the V(max) with a S(0.5) of 15 muM, and no changes in the K(m) for ATP-Mg. Glucose sensing in yeast generates Ca2+ transients involving Ca2+ influx from the external medium. We find that carbon-deprived cells respond to glucose with an immediate increase in mitochondrial ATP levels which is not observed in the presence of EGTA or in Sal1p-deficient cells. Moreover, we now report that during normal aerobic growth on glucose, yeast mitochondria import ATP from the cytosol and hydrolyse it through H+-ATP synthase. We identify two pathways for ATP uptake in mitochondria, the ADP/ATP carriers and Sal1p. Thus, during exponential growth on glucose, mitochondria are ATP consumers, as those from cells growing in anaerobic conditions or deprived of mitochondrial DNA which depend on cytosolic ATP and mitochondrial ATPase working in reverse to generate a mitochondrial membrane potential. In conclusion, the results show that growth on glucose requires ATP hydrolysis in mitochondria and recruits Sal1p as a Ca2+-dependent mechanism to import ATP-Mg from the cytosol. Whether this mechanism is used under similar settings in higher eukaryotes is an open question.
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- 2008
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23. Mg2+ deprivation elicits rapid Ca2+ uptake and activates Ca2+/calcineurin signaling in Saccharomyces cerevisiae.
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Wiesenberger G, Steinleitner K, Malli R, Graier WF, Vormann J, Schweyen RJ, and Stadler JA
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- Cytoplasm drug effects, Food Deprivation, Gene Expression Regulation, Fungal drug effects, Genome, Fungal drug effects, Magnesium pharmacology, RNA, Messenger genetics, RNA, Messenger metabolism, Saccharomyces cerevisiae cytology, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Transcription, Genetic drug effects, Calcineurin metabolism, Calcium metabolism, Calcium Signaling drug effects, Magnesium metabolism, Saccharomyces cerevisiae metabolism
- Abstract
To learn about the cellular processes involved in Mg(2+) homeostasis and the mechanisms allowing cells to cope with low Mg(2+) availability, we performed RNA expression-profiling experiments and followed changes in gene activity upon Mg(2+) depletion on a genome-wide scale. A striking portion of genes up-regulated under Mg(2+) depletion are also induced by high Ca(2+) and/or alkalinization. Among the genes significantly up-regulated by Mg(2+) starvation, Ca(2+) stress, and alkalinization are ENA1 (encoding a P-type ATPase sodium pump) and PHO89 (encoding a sodium/phosphate cotransporter). We show that up-regulation of these genes is dependent on the calcineurin/Crz1p (calcineurin-responsive zinc finger protein) signaling pathway. Similarly to Ca(2+) stress, Mg(2+) starvation induces translocation of the transcription factor Crz1p from the cytoplasm into the nucleus. The up-regulation of ENA1 and PHO89 upon Mg(2+) starvation depends on extracellular Ca(2+). Using fluorescence resonance energy transfer microscopy, we demonstrate that removal of Mg(2+) results in an immediate increase in free cytoplasmic Ca(2+). This effect is dependent on external Ca(2+). The results presented indicate that Mg(2+) depletion in yeast cells leads to enhanced cellular Ca(2+) concentrations, which activate the Crz1p/calcineurin pathway. We provide evidence that calcineurin/Crz1p signaling is crucial for yeast cells to cope with Mg(2+) depletion stress.
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- 2007
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24. The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome.
- Author
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Nowikovsky K, Froschauer EM, Zsurka G, Samaj J, Reipert S, Kolisek M, Wiesenberger G, and Schweyen RJ
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- Amino Acid Sequence, Animals, Calcium-Binding Proteins genetics, Cloning, Molecular, DNA, Complementary metabolism, Gene Deletion, Green Fluorescent Proteins, Homeostasis, Humans, Intracellular Membranes metabolism, Luminescent Proteins metabolism, Membrane Potentials, Membrane Proteins genetics, Microscopy, Confocal, Microscopy, Electron, Microscopy, Fluorescence, Mitochondrial Proteins, Molecular Sequence Data, Mutation, Phenotype, Plasmids metabolism, Potassium chemistry, Potassium Acetate pharmacology, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Sequence Homology, Amino Acid, Subcellular Fractions metabolism, Syndrome, Time Factors, Calcium-Binding Proteins metabolism, Membrane Proteins metabolism, Mitochondria metabolism, Multigene Family, Muscular Diseases genetics, Potassium metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
The yeast open reading frames YOL027 and YPR125 and their orthologs in various eukaryotes encode proteins with a single predicted trans-membrane domain ranging in molecular mass from 45 to 85 kDa. Hemizygous deletion of their human homolog LETM1 is likely to contribute to the Wolf-Hirschhorn syndrome phenotype. We show here that in yeast and human cells, these genes encode integral proteins of the inner mitochondrial membrane. Deletion of the yeast YOL027 gene (yol027Delta mutation) results in mitochondrial dysfunction. This mutant phenotype is complemented by the expression of the human LETM1 gene in yeast, indicating a functional conservation of LetM1/Yol027 proteins from yeast to man. Mutant yol027Delta mitochondria have increased cation contents, particularly K+ and low-membrane-potential Deltapsi. They are massively swollen in situ and refractory to potassium acetate-induced swelling in vitro, which is indicative of a defect in K+/H+ exchange activity. Thus, YOL027/LETM1 are the first genes shown to encode factors involved in both K+ homeostasis and organelle volume control.
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- 2004
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25. A specific role of the yeast mitochondrial carriers MRS3/4p in mitochondrial iron acquisition under iron-limiting conditions.
- Author
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Mühlenhoff U, Stadler JA, Richhardt N, Seubert A, Eickhorst T, Schweyen RJ, Lill R, and Wiesenberger G
- Subjects
- Adenosine Triphosphate metabolism, Blotting, Northern, Cytosol metabolism, Gene Deletion, Genome, Fungal, Heme metabolism, Intracellular Membranes metabolism, Membrane Potentials, Mitochondria metabolism, Mitochondrial Proteins, Oligonucleotide Array Sequence Analysis, Plasmids metabolism, Saccharomyces cerevisiae metabolism, Up-Regulation, Carrier Proteins physiology, Cation Transport Proteins, Iron metabolism, Repressor Proteins physiology, Saccharomyces cerevisiae Proteins physiology
- Abstract
The yeast genes MRS3 and MRS4 encode two members of the mitochondrial carrier family with high sequence similarity. To elucidate their function we utilized genome-wide expression profiling and found that both deletion and overexpression of MRS3/4 lead to up-regulation of several genes of the "iron regulon." We therefore analyzed the two major iron-utilizing processes, heme formation and Fe/S protein biosynthesis in vivo, in organello (intact mitochondria), and in vitro (mitochondrial extracts). Radiolabeling of yeast cells with 55Fe revealed a clear correlation between MRS3/4 expression levels and the efficiency of these biosynthetic reactions indicating a role of the carriers in utilization and/or transport of iron in vivo. Similar effects on both heme formation and Fe/S protein biosynthesis were seen in organello using mitochondria isolated from cells grown under iron-limiting conditions. The correlation between MRS3/4 expression levels and the efficiency of the two iron-utilizing processes was lost upon detergent lysis of mitochondria. As no significant changes in the mitochondrial membrane potential were observed upon overexpression or deletion of MRS3/4, our results suggest that Mrs3/4p carriers are directly involved in mitochondrial iron uptake. Mrs3/4p function in mitochondrial iron transport becomes evident under iron-limiting conditions only, indicating that the two carriers do not represent the sole system for mitochondrial iron acquisition.
- Published
- 2003
- Full Text
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