15 results on '"Wei-Wei Du"'
Search Results
2. Comparative effectiveness and safety of intravenous methylprednisolone and tacrolimus monotherapy in ocular myasthenia gravis with unsatisfactory prednisone responses: a retrospective study
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Kai-Yue Zhang, Wei-Wei Duan, Yue-Bei Luo, Yi Li, Jue Hu, and Huan Yang
- Subjects
Ocular myasthenia gravis ,Intravenous methylprednisolone ,Tacrolimus ,Monotherapy ,Clinical effectiveness ,Initial exacerbation ,Medicine - Abstract
Abstract Background Oral prednisone has been recognized as the first-line therapy for the treatment of ocular myasthenia gravis (OMG). However, its long-term use is complicated by numerous adverse effects and is ineffective for some OMG patients in reaching remission. This study aimed to evaluate the effectiveness and safety of intravenous methylprednisolone (IVMP) and tacrolimus monotherapy for OMG patients with unsatisfactory responses to conventional prednisone therapy. Methods We retrospectively reviewed 57 OMG patients who had not achieved satisfactory improvement after prednisone therapy and thereby received IVMP or tacrolimus monotherapy for at least 6 months. Ocular symptoms were evaluated by the ocular-quantitative MG (QMG) score at each time point. A ≥ 2-point fall in ocular QMG score was defined as the cut-off point to indicate clinical improvement. Logistic regression analysis was performed to identify factors associated with the efficacy of IVMP at discharge. Adverse events were recorded. Results Both IVMP and tacrolimus monotherapy demonstrated significant clinical efficacy, with no statistical differences observed at the study endpoint. The proportions of patients who reached the cut-off point for efficacy evaluation were higher in the IVMP group than in the tacrolimus group (1, 3, and 6 months: 51.7% (15/29) vs 12.0% (3/25), p = 0.002; 69.0% (20/29) vs 40.0% (10/25), p = 0.033; 69.0% (20/29) vs 46.4% (13/28), p = 0.085, respectively). Multivariate logistics analysis showed that high ocular QMG scores at baseline indicated favourable responses to IVMP treatment (OR = 1.781; 95% CI 1.066–2.975; p = 0.028). All the adverse events were transient and tolerable. Conclusion Our findings suggest that both IVMP and tacrolimus monotherapy hold promise as viable treatment options for OMG patients with unsatisfactory responses to oral prednisone. The study supports the safety and effectiveness of both therapies, with IVMP exhibiting faster improvement and favourable efficacy in patients with high ocular QMG scores.
- Published
- 2024
- Full Text
- View/download PDF
3. Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4
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Xiaolu Li, Li-Xiao Xu, Xiaolan Chen, Wei-Wei Du, Guang-Hui Qian, Chun Yang, Yi Wu, Jian Wang, Shaoyan Hu, He Zhao, Jian Pan, Fang Fang, Jun Lu, Xin Ding, Junli Ren, Zhi-Heng Li, Mei Li, Yunyun Xu, and Yan-Fang Tao
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Adult ,Male ,0301 basic medicine ,Cancer Research ,Cell cycle checkpoint ,Cell Survival ,MAP Kinase Signaling System ,Cell ,Biology ,neuroblastoma ,03 medical and health sciences ,Cell Line, Tumor ,Neuroblastoma ,medicine ,Humans ,Pyrroles ,Protein Kinase Inhibitors ,Cell Proliferation ,Neoplasm Staging ,Oncogene ,Kinase ,Cell Cycle ,apoptosis ,Articles ,General Medicine ,Cell cycle ,medicine.disease ,Up-Regulation ,Cell biology ,Gene Expression Regulation, Neoplastic ,PF-3758309 ,growth arrest ,030104 developmental biology ,medicine.anatomical_structure ,p21-Activated Kinases ,Oncology ,PAK4 ,Apoptosis ,Cancer cell ,Cancer research ,Pyrazoles ,Female - Abstract
Neuroblastoma is the most common extracranial solid childhood tumor. Despite the availability of advanced multimodal therapy, high-risk patients still have low survival rates. p21-activated kinase 4 (PAK4) has been shown to regulate many cellular processes in cancer cells, including migration, polarization and proliferation. However, the role of PAK4 in neuroblastoma remains unclear. In the present study, we demonstrated that PAK4 was overexpressed in neuroblastoma tissues and was correlated with tumor malignance and prognosis. To investigate the function of PAK4 in neuroblastoma, we used a small-molecule inhibitor that targets PAK4, that is, PF-3758309. Our results showed that PF-3758309 significantly induced cell cycle arrest at the G1 phase and apoptosis in neuroblastoma cell lines. Meanwhile, the inhibition of PAK4 by PF-3758309 increased the expression of CDKN1A, BAD and BAK1 and decreased the expression of Bcl-2 and Bax. In addition, we screened the target genes of PAK4 by PCR array and found that 23 genes were upregulated (including TP53I3, TBX3, EEF1A2, CDKN1A, IFNB1 and MAPK8IP2) and 20 genes were downregulated (including TNFSF8, Bcl2-A1, Bcl2L1, SOCS3, BIRC3 and NFKB1) after PAK4 inhibition by PF-3758309. Moreover, PAK4 was found to regulate the cell cycle and apoptosis via the ERK signaling pathway. In conclusion, the present study demonstrated, for the first time, the expression and function of PAK4 in neuroblastomas and the inhibitory effect of PF-3758309, which deserves further investigation as an alternative strategy for neuroblastoma treatment.
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- 2017
4. Inhibiting PLK1 induces autophagy of acute myeloid leukemia cells via mammalian target of rapamycin pathway dephosphorylation
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Yanhong Li, Guang-Hui Qian, Jian Wang, Shaoyan Hu, Xing Feng, Wei-Qi He, Li-Xiao Xu, Xie Yi, Jian Pan, Mei Li, Yan-Fang Tao, Zhi-Heng Li, Xiaolu Li, Wei-Wei Du, Yi-Ping Li, Gang Li, Fang Fang, Jun Lu, Junli Ren, and Yi Wu
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Male ,0301 basic medicine ,Cancer Research ,Cell Cycle Proteins ,Mice ,0302 clinical medicine ,hemic and lymphatic diseases ,Tumor Cells, Cultured ,Phosphorylation ,RNA, Small Interfering ,Child ,Reverse Transcriptase Polymerase Chain Reaction ,TOR Serine-Threonine Kinases ,RO3280 ,Myeloid leukemia ,Articles ,General Medicine ,Cell cycle ,Prognosis ,Cell biology ,Survival Rate ,Leukemia, Myeloid, Acute ,mTOR phosphorylation ,Oncology ,030220 oncology & carcinogenesis ,polo-like kinase 1 ,Female ,Signal Transduction ,autophagy ,Programmed cell death ,Blotting, Western ,Protein Serine-Threonine Kinases ,Biology ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Proto-Oncogene Proteins ,Biomarkers, Tumor ,Animals ,Humans ,RNA, Messenger ,Protein kinase A ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Neoplasm Staging ,BI2536 ,Autophagy ,pediatric acute myeloid leukemia ,Xenograft Model Antitumor Assays ,030104 developmental biology ,Cancer research ,K562 cells - Abstract
Decreased autophagy is accompanied by the development of a myeloproliferative state or acute myeloid leukemia (AML). AML cells are often sensitive to autophagy‑inducing stimuli, prompting the idea that targeting autophagy can be useful in AML cytotoxic therapy. AML NB4 cells overexpressing microtubule-associated protein 1 light chain 3-green fluorescent protein were screened with 69 inhibitors to analyze autophagy activity. AML cells were treated with the polo-like kinase 1 (PLK1) inhibitors RO3280 and BI2536 before autophagy analysis. Cleaved LC3 (LC3-II) and the phosphorylation of mammalian target of rapamycin (mTOR), adenosine monophosphate-activated protein kinase, and Unc-51-like kinase 1 during autophagy was detected with western blotting. Autophagosomes were detected using transmission electron microscopy. Several inhibitors had promising autophagy inducer effects: BI2536, MLN0905, SK1-I, SBE13 HCL and RO3280. Moreover, these inhibitors all targeted PLK1. Autophagy activity was increased in the NB4 cells treated with RO3280 and BI2536. Inhibition of PLK1 expression in NB4, K562 and HL-60 leukemia cells with RNA interference increased LC3-II and autophagy activity. The phosphorylation of mTOR was reduced significantly in NB4 cells treated with RO3280 and BI2536, and was also reduced significantly when PLK1 expression was downregulated in the NB4, K562 and HL-60 cells. We demonstrate that PLK1 inhibition induces AML cell autophagy and that it results in mTOR dephosphorylation. These results may provide new insights into the molecular mechanism of PLK1 in regulating autophagy.
- Published
- 2017
5. Practice of and Reflection on Medical Consortium by County-Level Hospitals Trusted to Provincial-Level Leading Hospitals
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Wei-Wei Du, Jin Chen, Hong Hu, and Jia-Long Zhu
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Reflection (computer programming) ,business.industry ,Service level ,Referral system ,Business ,Internal governance ,Public relations ,County level ,health care economics and organizations ,Municipal level ,Management - Abstract
Building new regional medical consortium in the model of trusting county-level hospitals to provincial-level large-scale public third-level grade-A hospitals can optimize the allocation of medical resources, promote two-way referral system and rational shunting of patients, and drive multi-sited practice of physicians. Furthermore, it can constantly improve regional medical service level, and achieve the goal of medical reform—comprehensive reform of county-level public hospitals. By introducing general situation where two county-level hospitals are trusted to Hubei Provincial People’s Hospital, this study analyses its operation performance and existing problems, and then puts forwards developmental countermeasures and recommendations for provincial-level hospitals to hold county-level hospitals in trust to build a medical consortium, including driving reorganization of medical resources, making clear roles of all the parties concerned, clarifying internal governance structure of hospitals under trust, reinforcing policy guidance, enhancing internal developmental force of trusteeship, intensifying supporting, and improving functions of medical consortium incrementally.
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- 2017
6. Practice and Reflections on 'Direct-billing Service' of Commercial Health Insurance in Hospitals: Experience of a Provincial-level Hospital in China
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Jia-Long Zhu, Jin Chen, Hong Hu, and Wei-Wei Du
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Service (business) ,Finance ,Actuarial science ,Health management system ,business.industry ,media_common.quotation_subject ,Self-insurance ,Big data ,010501 environmental sciences ,Payment ,01 natural sciences ,Municipal level ,03 medical and health sciences ,0302 clinical medicine ,Health insurance ,030212 general & internal medicine ,Business ,China ,health care economics and organizations ,0105 earth and related environmental sciences ,media_common - Abstract
The direct-billing collaboration between medical institutions and commercial health insurance institutions is an internationally applied method of commercial health insurance claims. This paper discusses the reasons why the direct-billing service of medical institutions is currently limited, including the trivial size of commercial health insurance and hospitals’ concern on cost control, by taking the practice of direct-billing service in a certain hospital in Wuhan as an example. It also summarizes the problems encountered in the operation, including the refusal to medical payment, the risks in excessive medical care, the increasing costs of medical institutions and acting against the equalization principle of medical service. The author then proposes several advices on the direct-billing service operated by hospitals, in terms of strengthening the risk control, taking part into the “Big Data” health management, reforming HIS system united with commercial insurance institutions and adjusting the pricing system of direct-billing service.
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- 2017
7. Molecular mechanism of G1 arrest and cellular senescence induced by LEE011, a novel CDK4/CDK6 inhibitor, in leukemia cells
- Author
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Jian Pan, Jian Wang, Na-Na Wang, Xing Feng, Yan-Fang Tao, Zhi-Heng Li, Yunyun Xu, Yi Wu, Guang-Hui Qian, Li-Xiao Xu, Mei Li, Xiaolu Li, Jun Lu, Junli Ren, Wei-Wei Du, Wei-Qi He, Fang Fang, Yanhong Li, Yi-Ping Li, and Shaoyan Hu
- Subjects
0301 basic medicine ,Senescence ,Cancer Research ,Cell ,Biology ,Cellular senescence ,Arraystar Human LncRNA array ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Genetics ,CDK4/6 ,LEE011 ,Propidium iodide ,Acute leukemia ,Leukemia ,Cell growth ,Cell cycle ,medicine.disease ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,Cyclin-dependent kinase 6 ,Primary Research - Abstract
Background Overexpression of cyclin D1 dependent kinases 4 and 6 (CDK4/6) is a common feature of many human cancers including leukemia. LEE011 is a novel inhibitor of both CDK4 and 6. To date, the molecular function of LEE011 in leukemia remains unclear. Methods Leukemia cell growth and apoptosis following LEE011 treatment was assessed through CCK-8 and annexin V/propidium iodide staining assays. Cell senescence was assessed by β-galactosidase staining and p16INK4a expression analysis. Gene expression profiles of LEE011 treated HL-60 cells were investigated using an Arraystar Human LncRNA array. Gene ontology and KEGG pathway analysis were then used to analyze the differentially expressed genes from the cluster analysis. Results Our studies demonstrated that LEE011 inhibited proliferation of leukemia cells and could induce apoptosis. Hoechst 33,342 staining analysis showed DNA fragmentation and distortion of nuclear structures following LEE011 treatment. Cell cycle analysis showed LEE011 significantly induced cell cycle G1 arrest in seven of eight acute leukemia cells lines, the exception being THP-1 cells. β-Galactosidase staining analysis and p16INK4a expression analysis showed that LEE011 treatment can induce cell senescence of leukemia cells. LncRNA microarray analysis showed 2083 differentially expressed mRNAs and 3224 differentially expressed lncRNAs in LEE011-treated HL-60 cells compared with controls. Molecular function analysis showed that LEE011 induced senescence in leukemia cells partially through downregulation of the transcriptional expression of MYBL2. Conclusions We demonstrate for the first time that LEE011 treatment results in inhibition of cell proliferation and induction of G1 arrest and cellular senescence in leukemia cells. LncRNA microarray analysis showed differentially expressed mRNAs and lncRNAs in LEE011-treated HL-60 cells and we demonstrated that LEE011 induces cellular senescence partially through downregulation of the expression of MYBL2. These results may open new lines of investigation regarding the molecular mechanism of LEE011 induced cellular senescence. Electronic supplementary material The online version of this article (doi:10.1186/s12935-017-0405-y) contains supplementary material, which is available to authorized users.
- Published
- 2017
8. A novel sphingosine kinase 1 inhibitor (SKI-5C) induces cell death of Wilms' tumor cells
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Zhi-Heng, Li, Yan-Fang, Tao, Li-Xiao, Xu, He, Zhao, Xiao-Lu, Li, Fang, Fang, Yi, Wu, Jun, Lu, Yan-Hong, Li, Wei-Wei, Du, Jun-Li, Ren, Yi-Ping, Li, Yun-Yun, Xu, Xing, Feng, Jian, Wang, Wei-Qi, He, and Jian, Pan
- Subjects
Original Article ,human activities - Abstract
Sphingosine kinase 1 (SphK1) is over-expressed in many cancers and therefore serves as a biomarker for cancer prognosis. SKI-5C is a new SphK1 inhibitor, and until now its molecular function in Wilms’ tumor cells remained unknown. Here, using CCK-8 and nude mice experiments we assessed cell growth in Wilms’ tumor cell lines (SK-NEP-1 and G401) in vitro and in vivo. We demonstrated that SphK1 is highly expressed in SK-NEP-1 and G401 cells, and through annexin V/propidium iodide staining and flow cytometry analysis, we detected cell apoptosis. Treatment with SKI-5C inhibited proliferation and induced apoptosis of SK-NEP-1 and G401 cells in a dose-dependent manner. Moreover, SKI-5C treatment inhibited the growth of SK-NEP-1 xenograft tumors in nude mice, with few side effects. Our microarray analysis revealed that SKI-5C-treated SK-NEP-1 cells mostly downregulated PRKACA and significantly inhibited phosphorylation of ERK1/2 and NF-κB p65. These results imply that SKI-5C induces apoptosis of SK-NEP-1 cells through the PRKACA/MAPK/NF-κB pathway. While, further research is required to determine the underlying details, these results provide new clues for the molecular mechanism of cell death induced by SKI-5C and suggest that SKI-5C may act as new candidate drug for Wilms’ tumor.
- Published
- 2016
9. Bilayer metallic nanofilms as broadband antireflection coatings in terahertz optical systems
- Author
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Xiao Li Tang, Huai Wu Zhang, Wei en Lai, Wei Wei Du, Yao hua Zhu, and Qi ye Wen
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Materials science ,business.industry ,Terahertz radiation ,Bilayer ,Physics::Optics ,Metal Nanoparticles ,Optical Devices ,Membranes, Artificial ,Atomic and Molecular Physics, and Optics ,Terahertz spectroscopy and technology ,Reflection (mathematics) ,Optical coating ,Optics ,Absorption, Physicochemical ,Attenuation coefficient ,Broadband ,Scattering, Radiation ,business ,Refractive index ,Terahertz Radiation ,Lenses - Abstract
We present the potential of ultrathin bilayer metallic nanofilms for use as broadband antireflection coatings in the terahertz frequency range. The metallic layers are modeled using a wave-impedance matching approach. The experimental and theoretical results are in good agreement. Further, a novel method using our broadband antireflection coatings is proposed to eliminate unwanted reflections that interfere with the important reflection from the sample in terahertz reflection measurement. The proposed method significantly improves the calculation of the optical properties of liquid and biological samples.
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- 2014
10. Relation between the Mechanical Behaviour of a High Strength Steel and the Microstructure in Gigacycle Fatigue
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Zhiyong Huang, Danièle Wagner, Claude Bathias, Wei Wei Du, Nanjing University of Aeronautics and Astronautics [Nanjing] (NUAA), Laboratoire Energétique Mécanique Electromagnétisme (LEME), and Université Paris Nanterre (UPN)
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Materials science ,Low alloyed steel ,Stress ratio ,Scanning electron microscope ,Surface treatment ,02 engineering and technology ,Shot peening ,01 natural sciences ,Carburizing ,0103 physical sciences ,General Materials Science ,Microstructure ,ComputingMilieux_MISCELLANEOUS ,010302 applied physics ,Inclusion ,[PHYS]Physics [physics] ,Mechanical Engineering ,Metallurgy ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Fatigue limit ,Piezoelectricity ,Gigacycle fatigue ,Mechanics of Materials ,Fatigue crack initiation ,Fracture (geology) ,0210 nano-technology - Abstract
Many components can reach or exceed 109 cycles in their service time. When fatigue life is beyond 106, the Wöhler S-N curve was always considered to be asymptotic in horizontal axis, but the fatigue behaviour over 106 cycles can not be neglected. It is not usual to carry out a fatigue test beyond 109 cycles due to the conventional fatigue test’s constraints, time consuming and expensive. High strength steel is widely applied in automobile, railway industry after surface treatment in order to improve performance of material in practice. Carburizing process hardens surface to increase wear and fatigue resistance and shot peening has a beneficial effect on the material fatigue strength from the surface residual compressive stresses. A piezoelectric gigacycle fatigue machine is used to do the tests in gigacycle regime on specimens with different surface treatments. The effect of different surface treatments is investigated in gigacycle regime at a frequency of 20KHz with a fixed stress ratio R=0.1 at room temperature. Moreover, Scanning Electron Microscopy (SEM) observations of fracture surfaces are analyzed to evaluate the mechanism of damage related to surface treatments, microstructure scored inclusion size. The role of inclusions and microstructure is emphasized at 109 cycles.
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- 2010
11. Molecular mechanism of G1 arrest and cellular senescence induced by LEE011, a novel CDK4/CDK6 inhibitor, in leukemia cells.
- Author
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Yan-Fang Tao, Na-Na Wang, Li-Xiao Xu, Zhi-Heng Li, Xiao-Lu Li, Yun-Yun Xu, Fang Fang, Mei Li, Guang-Hui Qian, Yan-Hong Li, Yi-Ping Li, Yi Wu, Jun-Li Ren, Wei-Wei Du, Jun Lu, Xing Feng, Jian Wang, Wei-Qi He, Shao-Yan Hu, and Jian Pan
- Subjects
CELLULAR aging ,CYCLIN-dependent kinase inhibitors ,LEUKEMIA ,GENETIC overexpression ,GALACTOSIDASES ,APOPTOSIS - Abstract
Background: Overexpression of cyclin D1 dependent kinases 4 and 6 (CDK4/6) is a common feature of many human cancers including leukemia. LEE011 is a novel inhibitor of both CDK4 and 6. To date, the molecular function of LEE011 in leukemia remains unclear. Methods: Leukemia cell growth and apoptosis following LEE011 treatment was assessed through CCK-8 and annexin V/propidium iodide staining assays. Cell senescence was assessed by β-galactosidase staining and p16INK4a expression analysis. Gene expression profiles of LEE011 treated HL-60 cells were investigated using an Arraystar Human LncRNA array. Gene ontology and KEGG pathway analysis were then used to analyze the differentially expressed genes from the cluster analysis. Results: Our studies demonstrated that LEE011 inhibited proliferation of leukemia cells and could induce apoptosis. Hoechst 33,342 staining analysis showed DNA fragmentation and distortion of nuclear structures following LEE011 treatment. Cell cycle analysis showed LEE011 significantly induced cell cycle G1 arrest in seven of eight acute leukemia cells lines, the exception being THP-1 cells. β-Galactosidase staining analysis and p16INK4a expression analysis showed that LEE011 treatment can induce cell senescence of leukemia cells. LncRNA microarray analysis showed 2083 differentially expressed mRNAs and 3224 differentially expressed lncRNAs in LEE011-treated HL-60 cells compared with controls. Molecular function analysis showed that LEE011 induced senescence in leukemia cells partially through downregulation of the transcriptional expression of MYBL2. Conclusions: We demonstrate for the first time that LEE011 treatment results in inhibition of cell proliferation and induction of G1 arrest and cellular senescence in leukemia cells. LncRNA microarray analysis showed differentially expressed mRNAs and lncRNAs in LEE011-treated HL-60 cells and we demonstrated that LEE011 induces cellular senescence partially through downregulation of the expression of MYBL2. These results may open new lines of investigation regarding the molecular mechanism of LEE011 induced cellular senescence. [ABSTRACT FROM AUTHOR]
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- 2017
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12. Antagonistic effect of early stage zinc on arsenic toxicity induced preterm birth during pregnancy: evidence from a rural Bangladesh birth cohort
- Author
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Yong-Yue Wei, Hui Huang, Yan-Kai Xia, Liang-Min Wei, Xin Chen, Ru-Yang Zhang, Wei-Wei Duan, Li Su, Mohammad L. Rahman, Mahmudur Rahman, Md. Golam Mostofa, Quazi Qamruzzaman, Wen-Hui Guo, Xian Sun, Hao Yu, Hong-Bing Shen, Zhi-Bin Hu, David C. Christiani, Feng Chen, and Yuan-Yuan Ji
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Medicine - Published
- 2021
- Full Text
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13. Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation
- Author
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Xiao-juan LI, Cheng-jin GUO, Wen-jing LU, Wei-wei DUAN, Miao ZHAO, Chun-ying MA, Jun-tao GU, and Kai XIAO
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wheat (Triticum aestivum L.) ,zinc finger protein ,Pi deprivation ,gene expression ,Agriculture (General) ,S1-972 - Abstract
Zinc finger protein (ZFP) genes comprise a large and diverse gene family, and are involved in biotic and abiotic stress responses in plants. In this study, a total of 126 ZFP genes classified into various types in wheat were characterized and subjected to expression pattern analysis under inorganic phosphate (Pi) deprivation. The wheat ZFP genes and their corresponding GenBank numbers were obtained from the information of a 4×44K wheat gene expression microarray chip. They were confirmed by sequence similarity analysis and named based on their homologs in Brachypodium distachyon or Oriza sativa. Expression analysis based on the microarray chip revealed that these ZFP genes are categorized into 11 classes according to their gene expression patterns in a 24-h of Pi deprivation regime. Among them, ten genes were differentially up-regulated, ten genes differentially down-regulated, and two genes both differentially up- and down-regulated by Pi deprivation. The differentially up- or down-regulated genes exhibited significantly more or less transcripts at one, two, or all of the checking time points (1, 6, and 24 h) of Pi stress in comparison with those of normal growth, respectively. The both differentially up- and down-regulated genes exhibited contrasting expression patterns, of these, TaWRKY70;5 showed significantly up-regulated at 1 and 6 h and down-regulated at 24 h whereas TaAN1AN20-8;2 displayed significantly upregulated at 1 h and downregulated at 6 h under deprivation Pi condition. Real time PCR analysis confirmed the expression patterns of the differentially expressed genes obtained by the microarray chip. Our results indicate that numerous ZFP genes in wheat respond to Pi deprivation and have provided further insight into the molecular basis that plants respond to Pi deprivation mediated by the ZFP gene family.
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- 2014
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14. Seven Sections Research Method Based on Engineering.
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Fan Li, Wei Wei Du, Run Qi Li, and Zhi Sen Wang
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- 2018
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15. Application of 4Rs principle in animal experiments for the research of human functions
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CAI Wei-wei, DU Bin, CHEN Jun-liang, TANG Shai-di, QIU Li-ying
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4rs principle ,animal ethics ,medical education ,Medicine - Abstract
Objective To evaluate 4Rs principle applied in animal experiments for research and to optimize training outcomes with animal model. Methods The 4Rs principle was applied in four parts of training process with animal models including “replacing animal experiments with human physiological experiments” “integrating experimental projects, reducing the use of animals” “optimizing experimental process for minimizing animal suffering” and “strengthening ethical concepts, responsibility in research with experimental animals”. After class, feedbacks from students were collected through questionnaire survey and the data was analyzed. Results There were 92.0% medical students showed sympathize for the laboratory animals in the process of animal experiments; took care of the welfare of experimental animals while achieving training objectives. Conclusions The 4Rs principle provides a new idea and method for animal experiment and cultivates learners' animal ethics.
- Published
- 2021
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