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Objective: To investigate the differences in chemical composition between ethanol extracts from Lonicera japonica flos (EELF) and L. japonica leaves (EELL) and their potential anti-aging components and mechanisms. Methods: The chemical compositions of EELF and EELL were determined by ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry (UPLC-QE-orbitrap-MS). The effect of feeding Escherichia coli OP50 culture containing different concentrations of EELF or EELL on the life span, mobility, reproductive capacity and oxidant stress resistance of Caenorhabditis elegans was investigated. The potential anti-aging mechanism of EELF and EELL was explored through network pharmacology. Results: A total of 122 compounds were identified in EELF and EELL, including 35 organic acids, 27 flavonoids, 37 iridoid compounds and 23 other compounds. The chemical compositions of EELF and EELL were roughly the same, and the contents of chlorogenic acid and total phenolic acid were also roughly same. The contents of total flavonoids, isochlorogenic acid C and luteolin in EELL were significantly higher than those in EELF, while the content of isochlorogenic acid A was significantly lower than that in EELF. EELF and EELL at all concentrations improved oxidative and thermal stress resistance, prolonged the life span, enhanced the motility and reproductive capacity of the nematode, and decreased the levels of reactive oxygen species (ROS) and lipofuscin. A total of 16 potential bioactive components from EELF and EELL and 59 potential anti-aging targets for them were identified, and the core targets were signal transducer and activator of transcription 3 (STAT3), serine/threonine kinase 1 (AKT1), epidermal growth factor receptor (EGFR), steroid receptor coactivator (SRC), cysteinyl aspartate specific proteinase 3 (CASP3), and tumor necrosis factor (TNF). The anti-aging effect could be exerted by suppressing cancer, reducing lipid and alleviating atherosclerosis through the regulation of the phosphatidyl-inositol 3-kinase/serine-threonine kinase (PI3K/AKT) signaling pathway. Conclusion: EELF and EELL have similar chemical compositions, exert similar anti-aging effects, and have multi-pathway and multi-target characteristics, which provides a reference for their deep development and utilization.

Since low-light images are of poor quality and carry noise, resulting in insufficient contrast and brightness, unclear details in the image, and the problem of high cost of obtaining paired low-light image datasets, a no-reference low-light image enhancement method for feature map enhancement is proposed under the influence of the Mach band effect of biological vision. Firstly, the model uses the enhanced filter block (EFB) to enhance the feature of images and feature maps, which can suppress noise and enhance the feature details to improve the ability of the network to learn features. Then, skip connection is combined with efficient spatial attention (ESA) module to extract global context information and local regional features by fusing enhanced shallow features and deep features, which effectively retains image color information, avoids detail loss, and improves the generalization ability of the network. Finally, the pixel estimation curve is used to adjust the dynamic range of the pixels in the low-light image and enhance the brightness of the low-light image. Experimental results show that the PSNR, SSIM, LPIPS and NIQE of the image processed by the algorithm reach 17.709 dB, 0.657, 0.239 and 3.486 respectively. Compared with the existing mainstream algorithms, it can better achieve the purpose of image enhancement, and effectively improves the image brightness and detail information, while maintaining the naturalness of the image.

Autologous platelet concentrate (APC) is a platelet concentrate obtained by centrifugation of human or animal blood, mainly includes first-generation platelet concentrate platelet-rich plasma (PRP) and second-generation platelet concentrate platelet-rich fibrin (PRF), which is widely applied in varies fields such as dental implants, bone defect repairing and burn plastic surgery because of its role in wound healing and tissue repair. In recent years, autologous platelet concentrate has been applied to improve ovarian function, promote the repair of endometrial hyperplasia, treat cervical and vulvo-related lesions, and has achieved significant efficacy. However, there is a lack of effective integration of PRP and PRF research results in the field of gynecology. This paper systematically and comprehensively review the preparation, biological properties and applications in various gynecological diseases, in order to provide clinicians with new treatment ideas for gynecological diseases and reference for the further application of APC.

Acute myeloid leukemia (AML) is a myeloid malignancy with generally high mortality. Although recent advances in AML research have revealed that circRNAs play significant roles in AML progression, our understanding of the leukemogenic mechanism of circRNAs remains very limited. In this study, increased expression of hsa_circ_0013880 was observed in bone marrow mononuclear cells (BMNCs) of AML patients. Overexpression of hsa_circ_0013880 promotes AML cell proliferation and migration and reduces cell apoptosis. Mechanistically, hsa_circ_0013880 could elevate the expression of USP32, a deubiquitinating enzyme that is highly expressed in the BMNCs of AML patients. Given the deubiquitination function of USP32, we further hypothesize that USP32 may mediate the malignant behaviors of AML cells by regulating the stability of Ras-related protein (Rap1b). At the molecular level, we find that silencing of USP32 increases ubiquitinated Rap1b. Overexpression of Rap1b restores the effects of USP32 knockdown, which further verifies our hypothesis. In addition, we propose another hypothesis that a potential regulatory network among hsa_circ_0013880, miR-148a-3p/miR-20a-5p and USP32 might exist in the development of AML, according to bioinformatics website predictions and our preliminary experimental verification. Overall, our findings will enrich the understanding of the hsa_circ_0013880/USP32/Rap1b axis in AML development, which may contribute to the development of novel therapeutic strategies for AML.