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C22H22N4O8Mn, monoclinic, P21/c (no. 14), a = 12.7620(10) Å, b = 14.980(2) Å, c = 13.2910(11) Å, β = 120.070(5)°, V = 2198.9(4) Å3, Z = 4, Rgt(F) = 0.0594, wRref(F2) = 0.1345, T = 293(2) K.

The role of vaccine-induced anti-V2 Abs was tested in three protection experiments in rhesus macaques. In an experiment using immunogens similar to those in the RV144 vaccine trial (Anti-envelope [Env]), nine rhesus macaques were coimmunized with gp16092TH023 DNA and SIV gag and gp120A244 and gp120MN proteins. In two V2-focused experiments (Anti-V2 and Anti-V2 Mucosal), nine macaques in each group were immunized with V1V292TH023 DNA, V1V2A244 and V1V2CasaeA2 proteins, and cyclic V2CaseA2 peptide. DNA and protein immunogens, formulated in Adjuplex, were given at 0, 4, 12, and 20 weeks, followed by intrarectal SHIVBaL.P4 challenges. Peak plasma viral loads (PVL) of 106-107 copies/ml developed in all nine sham controls. Overall, PVL was undetectable in one third of immunized macaques, and two animals tightly controlled the virus with the Anti-V2 Mucosal vaccine strategy. In the Anti-Env study, Abs that captured or neutralized SHIVBaL.P4 inversely correlated with PVL. Conversely, no correlation with PVL was found in the Anti-V2 experiments with nonneutralizing plasma Abs that only captured virus weakly. Titers of Abs against eight V1V2 scaffolds and cyclic V2 peptides were comparable between controllers and noncontrollers as were Ab-dependent cellular cytotoxicity and Ab-dependent cell-mediated virus inhibition activities against SHIV-infected target cells and phagocytosis of gp120-coated beads. The Anti-Env experiment supports the role of vaccine-elicited neutralizing and nonneutralizing Abs in control of PVL. However, the two V2-focused experiments did not support a role for nonneutralizing V2 Abs alone in controlling PVL, as neither Ab-dependent cellular cytotoxicity, Ab-dependent cell-mediated virus inhibition, nor phagocytosis correlated inversely with heterologous SHIVBaL.P4 infection.

An inductively coupled plasma atomic emission spectrometry (ICP - AES) method was developed for the determination of 12 impurity elements (Cd, Mn, Cr, Cu, Fe, Ca, Zn, Al, Co, Nd, Pr and Ce) in nickel plating solution for Nd - Fe - B. The optimum working parameters of the instrument and the analytical spectral lines of each element were determined. By using matrix matching and simple bath dilution, the spectral interference to the elements to be detected caused by a large number of matrix substances in the bath was eliminated. Results showed that the linear relationship of these 12 elements was good, the linear correlation coefficient R > 0.999, the detection limit was 0.005～0.690 μg/mL, the results of experiments of precision and recovery showed that this method had the advantages of simple, rapid and accurate, meeting the requirements of daily detection of impurity elements in Nd - Fe - B nickel plating solution.

Objective: To examine cognitive deficits and associated brain activity in fragile X-associated tremor/ataxia syndrome (FXTAS) patients with parkinsonism (FXTp+), in relation to FXTAS patients without parkinsonism (FXTp-), and normal elderly controls (NC). Methods: Retrospective reviews were performed in 65 FXTAS patients who participated in the event-related brain potential (ERP) study and also had either a videotaped neurological examination or a neurological examination for extrapyramidal signs. Parkinsonism was defined as having bradykinesia with at least one of the following: rest tremor, postural instability, hypermyotonia, or rigidity. Eleven FXTp+ patients were identified and compared to 11 matched FXTp- and 11 NC. Main ERP measures included the N400 congruity effect, N400 repetition effect, and the late positive component (LPC) repetition effect. Results: When compared with FXTp- and NC, the FXTp+ group showed more severe deficits in executive function, cued-recall, recognition memory, along with a significantly reduced N400 repetition effect (thought to index semantic processing and verbal learning/memory) which was correlated with poorer verbal memory. Across all patients, FMR1 mRNA levels were inversely correlated with delayed recall on the California Verbal Learning Test (CVLT). Interpretation: The findings of more prominent executive dysfunction and verbal learning/memory deficits in FXTp+ than FXTp- are consistent with findings in Parkinson's disease (PD), and may indicate that concomitant and/or synergistic pathogenetic mechanisms associated with PD play a role in FXTAS. These results have implications not only for understanding the cognitive impairments associated with the parkinsonism subtype of FXTAS, but also for the development of new interventions for these patients.

Microbial remediation has become a prominent focus in soil pollution control due to its environmental friendliness, cost-effectiveness, and high efficiency. The effectiveness of microbial remediation is rooted in the interactions between microbial metabolic activities and the soil environment. Various microorganisms employ distinct mechanisms for pollutant treatment, including surface adsorption, intracellular accumulation, and biomineralization. Using the Web of Science Core Collection database, tools such as CiteSpace 6.1.R6, VOSviewer 1.6.20, and HistCite Pro were employed to conduct a quantitative analysis of several key aspects: the volume and thematic distribution of research papers on microbial remediation of soils, the cooperative networks between countries and institutions, the leading journals, major research hotspots, and emerging trends. The analysis reveals that utilizing microbial regulatory mechanisms and functions to remediate inorganic pollutants, such as heavy metals, and organic pollutants, such as PAHs, is becoming a significant frontier in future research. This study provides a valuable reference for scholars aiming to understand the current status of microbial research in soil remediation, both domestically and internationally. It also offers guidance for developing efficient, sustainable, and safe remediation strategies while identifying directions for future innovative research. The specific results are as follows: (1) China, the USA, India, and other countries have a high frequency of citations in this field, and the research is more in-depth. (2) More and more attention has been paid to the use of microbial remediation of contaminated soil in the world, mainly in Environmental Sciences. (3) Major publications include Chemosphere, Journal of Hazardous Materials, and Science of The Total Environment. In the key literature, the use of microorganisms to restore the soil environment and the combination of microorganisms and plants to repair soil contaminated by heavy metals occupy a high proportion. (4) The key areas of focus include the application of microorganisms in soil inorganic pollution remediation, the application of microorganisms in remediation of soil organic pollution (crude oil and polycyclic aromatic hydrocarbons (PAHs)), and the contribution of microorganisms to soil pollutant degradation and toxicity assessment systems. The research and development of combined microbial remediation technology is the current research hotspot in the field of soil remediation, focusing on the symbiosis between mycorrhizal fungi and plant roots, the enhancement in the ability of microorganisms to absorb and degrade pollutants and their tolerance, and the interaction mechanism between indigenous microorganisms and plants. [ABSTRACT FROM AUTHOR]