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Chile’s mining industry, a global leader in copper production, faces challenges due to increasing volumes of mining waste, particularly Waste Rock Dumps (WRD) and Leaching Waste Dumps (LWD). The National Service of Geology and Mining (SERNAGEOMIN) requires assessment of the physical stability (PS) of these facilities, but current methods are hindered by data scarcity and resource constraints. This study proposes a simplified evaluation methodology using first-order parameters from open-access data. By integrating Geographic Information Systems (GIS) and Artificial Intelligence (AI)—utilizing models like YOLOv11 and convolutional neural networks—we automate the detection and characterization of WRD and LWD from satellite imagery, extracting critical parameters for PS assessment. This approach reduces analysis time and minimizes human error. Validated in the Antofagasta Region, Chile’s primary mining area, we identified and evaluated 70 WRD and 54 LWD. The results demonstrate the effectiveness of prioritizing deposits based on potential risk, enhancing SERNAGEOMIN’s capacity for supervision. The successful application suggests scalability to other mining regions and adaptability to different facility types, including tailings storage facilities. This work offers a practical tool to improve safety and risk management in the mining industry, addressing critical challenges in PS evaluation under current regulatory constraints.

Abstract Background Recent evidence from both randomized controlled trials and cohort studies in adults suggests that plasma remnant cholesterol (RC) levels predict cardiovascular disease. In children, studies are scarce, although high levels of RC might represent a marker of early atherosclerotic damage. Thus, the aim of this study was to explore the cardiometabolic risk associated with RC, which extends beyond low-density lipoprotein cholesterol (LDL-c) in children. Methods Cardiometabolic risk factors (plasma insulin levels, homeostatic model assessment for insulin resistance, mean arterial blood pressure (MAP), waist circumference (WC), and cardiorespiratory fitness (CRF)) were examined in 3417 Spanish schoolchildren aged 8–11 years. The children were categorized into four subgroups (low vs. high) based on the cutoff of ≥ 110 mg/dL for LDL-c and of ≥ 15 mg/dL for RC to define higher levels, and ANCOVA models were applied to assess the role of both lipid parameters in cardiometabolic risk. Additionally, multilevel mixed-effects generalized linear regression models were used to assess the associations of RC or LDL-c with cardiometabolic risk factors and to examine whether the associations between RC and these factors varied in children with low or high LDL-c levels. Results Children in the high-RC subgroups, specifically those with low LDL-c/high RC and high LDL-c/high RC, presented significantly greater insulin levels and WC than did their peers in the low-RC subgroups. RC was more strongly associated with cardiometabolic risk factors than LDL-c (insulin β = 2.073/ − 0.026; HOMA-IR β = 0.451/ − 0.002; MAP β = 1.214/0.300; WC β = 2.842/1.058; and CRF β = − 0.316/ − 0.194 for RC and LDL-c, respectively). Furthermore, RC exhibited associations even in children with low LDL-c levels: insulin (β = 2.305; p

Abstract Background High-field magnetic resonance imaging (MRI) is a powerful diagnostic tool but can induce unintended physiological effects, such as nystagmus and dizziness, potentially compromising the comfort and safety of individuals undergoing imaging. These effects likely result from the Lorentz force, which arises from the interaction between the MRI's static magnetic field and electrical currents in the inner ear. Yet, the Lorentz force hypothesis fails to explain observed eye movement patterns in healthy adults fully. This study explores these effects and tests whether the Lorentz force hypothesis adequately explains magnetic vestibular stimulation. Methods We developed a mathematical model integrating computational fluid dynamics, fluid-structure interaction solvers, and magnetohydrodynamic equations to simulate the biomechanical response of the cristae ampullares. Using high-resolution micro-CT data of the human membranous labyrinth, we ensured anatomical accuracy. Experimental validation involved measuring horizontal, vertical, and torsional slow-phase eye movements in healthy subjects exposed to varying magnetic field intensities and head positions. Results Our model accurately replicates observed nystagmus patterns, predicting slow-phase eye velocities that match experimental data. Results indicate that Lorentz force-induced stimulation of individual cupulae explains variability in eye movements across different magnetic field intensities and head orientations. Conclusions This study empirically supports the Lorentz force hypothesis as a valid explanation for magnetic vestibular stimulation, offering new insights into the effects of high-field MRI on the vestibular system. These findings provide a foundation for future research and improved clinical practices.

Abstract Background Active learning, on average, increases student performance in STEM courses. Yet, there is also large variation in the effectiveness of these implementations. A consistent goal of active learning is moving students towards becoming active constructors of their knowledge. This emphasis means student engagement is of central importance. Thus, variation in student engagement could help explain variation in outcomes from active learning. In this study, we employ Pekrun’s Control–Value Theory to examine the impact of four aspects of course social and cultural environments on student engagement. This theory posits that social and cultural features of the course environment influence students’ appraisals of their ability to control their academic outcomes from the course and the value they see in those outcomes. Control and value in turn influence the emotions students experience in the course and their behaviors. We selected four features of the course environment suggested in the literature to be important in active learning courses: course goal structure, relevance of course content, students’ trust in their instructor, and perceived course competition. Results We surveyed students in 13 introductory STEM courses. We used structural equation modeling to map how features of the course environment related to control, value, and academic emotions, as well as how control, value, and academic emotions influenced engagement. We found engagement was positively related to control and value as well as the emotion of curiosity. Engagement was negatively related to the emotion of boredom. Importantly, features of the course environment influenced these four variables. All features influenced control: goal structure, relevance, and instructor trust increased it, while competition decreased it. All features except competition were related positively to value. Relevance and instructor trust increased curiosity. Goal structure, relevance, and instructor trust all reduced boredom, while competition increased it. Conclusion Overall, our study suggests that the way instructors structure the social and cultural environment in active learning courses can impact engagement. Building positive instructor–student relationships, reducing course competition, emphasizing mastery and the relevance of the course to students can all increase engagement in course activities.

Abstract This article presents a new crossover operator for problems with an underlying graph structure where edges point to prospective interdependence relationships between decision variables and neighborhoods shape the definition of the global objective function via a sum of different expressions, one for each neighborhood. The main goal of this work is to propose a crossover operator that is broadly applicable, adaptable, and effective across a wide range of problem settings characterized by objective functions that are expressed in terms of graph neighbourhoods with interdependent and related variables. Extensive experimentation has been conducted to compare and evaluate the proposed crossover operator with both classic and specialized crossover operators. More specifically, the crossover operators have been tested under a variety of graph types, which model how variables are involved in interdependencies, different types of expressions in which interdependent variables are combined, and different numbers of decision variables. The results suggest that the new crossover operator is statistically better or at least as good as the best-performing crossover in 75% of the families of problems tested.

The Trait Meta-Mood Scale is widely used to assess individual differences in perceived emotional intelligence. This instrument measures self-reported emotional attention, clarity, and repair. The present study analyzed the psychometric properties including reliability and validity of a Polish version of the TMMS-24 for adolescents including a broad sample of Polish 16 to 22 years old participants (40.3% girls). Results of a confirmatory factor analysis showed that, as its original version, TMMS-24 has a three-factor structure (attention, clarity, and repair), an adequate internal consistency and correlations among the three factors. In terms of relations with other variables, we found evidence of convergent validity with emotional empathy, and discriminant validity with aggressive behavior. These findings suggest that the Polish version of the TMMS-24 for adolescents is a valid instrument for evaluating perceived emotional intelligence in Polish-speaking adolescents that can be used for basic and applied research, and for the evaluation of programs for the promotion of socioemotional competencies.

This study reports for the first time the presence of Rhizoprionodon terraneovae neonates in a coastal lagoon in the southeastern Gulf of Mexico. The observations suggest the presence of neonates was due to the salinity values being similar to those of the coastal zone (32 ppt) and that they may have been attracted by the high presence of penaeid shrimp. The presence of this species in this lagoon supports the knowledge of the biology and ecology of the species, which will contribute to its proper fishing management.

Abstract A lensless compact arrangement based on digital in-line holography under Gabor’s regime is proposed as a novel contactless method to assess the profile of multifocal intraocular lenses (MIOLs) which are conformed by several diffractive rings. Diffractive MIOLs are a widely adopted ophthalmologic option for the correction of presbyopia in patients undergoing cataract surgery. The MIOL optical design might introduce non-negligible optical performance differences between lenses as well as the introduction of undesirable photic phenomena (such as halos and glare) affecting the vision of users. Therefore, the customized topographic control of each manufactured MIOL model, along with the advancement of optical simulation routines, is increasingly necessary to provide users with optimized performance of these implanted optics, as well as predictable and realistic expectations of their future vision with these solutions. In this manuscript, experimental results of the reconstruction of different smooth and highly edged diffractive profiles from a pair of commercially available MIOLs are presented. Besides, a study evaluating the convergence and robustness of the proposed iterative phase-retrieval routine based on a modified classical Gerchberg-Saxton algorithm is performed. These results provide experimental validation of the proposed technique for accurately measuring the optical profiles of MIOLs.

Abstract Symbolic anchoring is an important topic in robotics, as it enables robots to obtain symbolic knowledge from the perceptual information acquired through their sensors and maintain the link between that knowledge and the sensory data. In cognitive-based robots, this process of transforming sub-symbolic data generated by sensors to obtain and maintain symbolic knowledge is still an open problem. To address this issue, this paper presents SAILOR, a framework for symbolic anchoring integrated into ROS 2. SAILOR aims to maintain the link between symbolic data and perceptual data in real robots over time. It provides a semantic world modeling approach using two deep learning-based sub-symbolic robotic skills: object recognition and matching function. The object recognition skill allows the robot to recognize and identify objects in its environment, while the matching function enables the robot to decide if new perceptual data corresponds to existing symbolic data. This paper describes the proposed method and the development of the framework, as well as its integration in MERLIN2 (a hybrid cognitive architecture fully functional in robots running ROS 2) and the validation of SAILOR using public datasets and a real-world scenario.

Abstract Background Social support is relevant to studying well-being, quality of life, and health during aging, particularly in people over 50. Therefore, brief instruments that allow its measurement within the clinical evaluation and research processes are necessary. The ENRICH Social Support Scale (ESSI) is a brief and easy-to-use instrument that measures the perception of social support; however, its psychometric properties in people over 50 in the Chilean context have yet to be tested. Method This study had a non-experimental, longitudinal panel design in which a sample of 303 people over 50 years of age (M = 55.14 years, SD = 5.19; 52% women) were administered a survey incorporating sociodemographic variables, the ESSI and the Newsom Social Negativity Scale. 277 participants completed the follow-up survey (M = 56.75 years, SD = 5.15; 54% women). Confirmatory factor analysis (CFA), structural equation modeling (SEM), invariance analysis, and internal consistency tests were performed to determine the psychometric properties. Results The six items from the scale load in a single-factor model obtained an excellent fit to the data and reliability coefficients (α = 0.902; ω = 0.904). The SEM analysis evidenced an inverse correlation between the ESSI and Newsom's social negativity scale, demonstrating evidence of construct validity. Furthermore, in the sex invariance analyses, the scalar level was reached, implying that the items’ meanings are the same for men and women. Finally, the residual level was reached in the temporal invariance analyses, which means that the scale items are consistent in time. Conclusions The ESSI is a reliable and valid tool to be used in the Chilean context both in the clinical setting and in research on people over 50. The evidence obtained supports its usability to appropriately measure the perception of social support, which is relevant considering that it has been associated with reports of higher quality of life and lower mortality and morbidity during aging.

Abstract Background The main application of cork is the production of stoppers for wine bottles. Cork sometimes contains 2,4,6-trichloroanisole, a compound that, at a concentration of ng/L, produces an unpleasant musty odor that destroys the organoleptic properties of wine and results in enormous economic losses for wineries and cork industries. Cork can exhibit a defect known as yellow stain, which is associated with high levels of 2,4,6-trichloroanisole. We describe how the microbiota of cork and yellow stain define a novel mechanism that explains the formation of chlorophenols and chloroanisoles (including 2,4,6-trichloroanisole) from p-hydroxybenzoate produced during lignin and/or suberin breakdown. Results Electron microscopy revealed that cork affected by yellow stain exhibited significant structural degradation. This deterioration was attributed to the presence of higher microbial populations compared to those found in standard cork. Cork microbiota is rich in filamentous fungi able to metabolize lignin. A metataxonomic analysis confirmed that yellow stain contained significantly greater populations of fungal species belonging to Absidia, Geomyces, Mortierella, Mucor, Penicillium, Pseudogymnoascus, Talaromyces, and Umbelopsis. It also contained significantly greater amounts of bacteria belonging to Enterobacterales, Streptosporangiales, Tepidisphaerales, Pseudomonas, and several members of Burkholderiaceae, particularly species of the Burkholderia-Caballeronia-Paraburkholderia group. The extraction of aromatic compounds from cork samples allowed the identification of several compounds typically observed following lignin depolymerization. Notably, p-hydroxybenzoic acid and phenol were detected. Two strains of the genus Streptomyces isolated from yellow stain were able to biotransform p-hydroxybenzoate into phenol in resting cell assays. Phenol could be efficiently chlorinated in vitro to produce 2,4,6-trichlorophenol by a fungal chloroperoxidase, an enzymatic activity commonly found in filamentous fungi isolated from cork. Finally, as has been widely demonstrated before, 2,4,6-trichlorophenol can be efficiently O-methylated to 2,4,6-trichloroanisole by many of fungi that inhabit cork. Conclusions Chlorophenols and chloroanisoles can be produced de novo in cork from p-hydroxybenzoate generated by the microbial biodegradation of lignin and/or suberin through the participation of different types of microorganisms present in cork. The natural origin of these compounds, which are of great interest for the chlorine cycle and represent a new source of environmental contamination that differs from that caused by human activity, is described. Video Abstract

Takayasu's Arteritis (TA) is a rare, chronic large-vessel vasculitis that can lead to severe cardiac complications and life-threatening outcomes. Early diagnosis is essential for improving patient prognosis, but its nonspecific clinical presentation and laboratory findings often cause delays. We present a 34-year-old woman with a history of heart murmur who presented with chest pain but no additional symptoms. Imaging revealed aortic regurgitation, ventricular septal defect, myocardial ischemia, pericarditis, aortic wall thickening, and multivessel stenoses, leading to a diagnosis of Takayasu's Arteritis, treated with coronary bypass and aortic tube graft surgery. Takayasu's Arteritis should be included in the differential diagnosis of patients presenting with atypical clinical features and cardiac involvement, particularly in cases with valvular disease. This case highlights the essential role of multimodal imaging in the detection and management of TA.

Abstract Background Patients with steatotic liver disease may show mild cognitive impairment. Rats with mild liver damage reproduce this cognitive impairment, which is mediated by neuroinflammation that alters glutamate neurotransmission in the hippocampus. Treatment with extracellular vesicles (EV) from mesenchymal stem cells (MSC) reduces neuroinflammation and improves cognitive impairment in different animal models of neurological diseases. TGFβ in these EVs seems to be involved in its beneficial effects. The aim of this work was to assess if MSCs-EVs may improve cognitive impairment in rats with mild liver damage and to analyze the underlying mechanisms, assessing the effects on hippocampal neuroinflammation and neurotransmission. We also aimed to analyze the role of TGFβ in the in vivo effects of MSCs-EVs. Methods Male Wistar rats with CCl4-induced mild liver damage were treated with EVs from unmodified MSC or with EVs derived from TGFβ—silenced MSCs and its effects on cognitive function and on neuroinflammation and altered neurotransmission in the hippocampus were analysed. Results Unmodified MSC-EVs reversed microglia activation and TNFα content, restoring membrane expression of NR2 subunit of NMDA receptor and improved object location memory. In contrast, EVs derived from TGFβ—silenced MSCs did not induce these effects but reversed astrocyte activation, IL-1β content and altered GluA2 AMPA receptor subunit membrane expression leading to improvement of learning and working memory in the radial maze. Conclusions EVs from MSCs with TGFβ silenced induce different effects on behavior, neuroinflammation and neurotransmitter receptors alterations than unmodified MSC-EVs, indicating that the modification of TGFβ in the MSC-EVs has a notable effect on the consequences of the treatment. This work shows that treatment with MSC-EVs improves learning and memory in a model of mild liver damage and MHE in rats, suggesting that MSC-EVs may be a good therapeutic option to reverse cognitive impairment in patients with steatotic liver disease.

Abstract Background Animal-borne sensors (‘bio-loggers’) can record a suite of kinematic and environmental data, which are used to elucidate animal ecophysiology and improve conservation efforts. Machine learning techniques are used for interpreting the large amounts of data recorded by bio-loggers, but there exists no common framework for comparing the different machine learning techniques in this domain. This makes it difficult to, for example, identify patterns in what works well for machine learning-based analysis of bio-logger data. It also makes it difficult to evaluate the effectiveness of novel methods developed by the machine learning community. Methods To address this, we present the Bio-logger Ethogram Benchmark (BEBE), a collection of datasets with behavioral annotations, as well as a modeling task and evaluation metrics. BEBE is to date the largest, most taxonomically diverse, publicly available benchmark of this type, and includes 1654 h of data collected from 149 individuals across nine taxa. Using BEBE, we compare the performance of deep and classical machine learning methods for identifying animal behaviors based on bio-logger data. As an example usage of BEBE, we test an approach based on self-supervised learning. To apply this approach to animal behavior classification, we adapt a deep neural network pre-trained with 700,000 h of data collected from human wrist-worn accelerometers. Results We find that deep neural networks out-perform the classical machine learning methods we tested across all nine datasets in BEBE. We additionally find that the approach based on self-supervised learning out-performs the alternatives we tested, especially in settings when there is a low amount of training data available. Conclusions In light of these results, we are able to make concrete suggestions for designing studies that rely on machine learning to infer behavior from bio-logger data. Therefore, we expect that BEBE will be useful for making similar suggestions in the future, as additional hypotheses about machine learning techniques are tested. Datasets, models, and evaluation code are made publicly available at https://github.com/earthspecies/BEBE , to enable community use of BEBE.

The Hen Harrier (Circus cyaneus) is a medium-sized raptor with a broad distribution across the Palearctic. In Spain, Hen Harrier behaviour is diverse due to being at the southern limit of its distribution, and the margins of distributions tend to show greater variability in the strategies and behaviours of animals. This study focused on juvenile dispersal, using GPS/GSM data from seven individuals to define movement patterns, compare variables between sexes, and analyse differences between the first and second years of dispersal. To analyse the movements during each annual period, six variables were considered, namely the mean distance from nest location, maximum distance from nest location, mean daily distance travelled, total distance travelled, 95% weekly kernel, and 95% total kernel. In their first year after leaving the nest, the Hen Harriers began dispersal movements on 21 August ± 34.41 days, with highly variable distances and durations among individuals. They travelled an average total of 6774.66 ± 5360.46 km over the two first years, with some significant differences between sexes and periods in terms of movement patterns, particularly in the daily and total distances travelled. Overall, the maximum distance from nest location and the total distance travelled were greater in the first year of dispersal than in the second year, indicating, as expected, an improved understanding of their environment and more efficient movements. The juvenile dispersal behaviour of the Hen Harrier is highly variable and represents the most vulnerable season for survival as the birds navigate new and unexplored territories.

This paper analyses the market entry strategy of the Spanish wineries and their destination markets. For this purpose, channel adjustment was analysed. The novel aspect of this research is the use of exports on a winery level, basing the explanation of their export performance on a combination of internal decisions of the companies, and characteristics of the wine destination markets, in accordance with the purchasing patterns of their consumers. The results revealed the importance of strategic adjustment between winery and market destination, even though the importance of adjustment depends on the level of exports. Thus, channel adjustment is important for the probability of export and export intensity.

Abstract We present novel fluorescent cholesteryl probes (CNDs) with a modular design based on the solvatochromic 1,8-phthalimide scaffold. We have explored how different modules—linkers and head groups—affect the ability of these probes to integrate into lipid membranes and how they distribute intracellularly in mouse astrocytes and fibroblasts targeting lysosomes and lipid droplets. Each compound was assessed for its solvatochromic behavior in organic solvents and model membranes. Molecular dynamics simulations and lipid partitioning using giant unilamellar vesicles showed how these analogs behave in model membranes compared to cholesterol. Live-cell imaging demonstrated distinct staining patterns and cellular uptake behaviors, further validating the utility of these probes in biological systems. We compared the empirical results with those of BODIPY-cholesterol, a well-regarded fluorescent cholesterol analog. The internalization efficiency of fluorescent CND probes varies in different cell types and is affected mainly by the head groups. Our results demonstrate that the modular design significantly simplifies the creation of fluorescent cholesteryl probes bearing distinct spectral, biophysical, and cellular targeting features. It is a valuable toolkit for imaging in live cells, measuring cellular membrane dynamics, and studying cholesterol-related processes.

The first case of xanthochromism in a female common snook, Centropomus undecimalis (Bloch, 1792), in the southern Gulf of Mexico is described. The specimen supporting this record was caught off the coast of Tabasco, Mexico and it represents the first fish case of this abnormal coloration in the Gulf of Mexico. The analyzed specimen measured 774 mm in total length. Its body was entirely orange with a lighter shade of orange on the belly, while the normal coloration of this fish species is dark to opaque grey with yellow to green tints on the dorsal part, silvery lateral side, white belly, and black lateral line. It is uncertain what caused this abnormality, but it is hypothesized that multiple environmental stressors, natural and anthropogenic, were responsible for the appearance of this particular abnormality and other similar cases in the area. Additional sampling and long-term monitoring are needed to determine the possible causes and their ecological impacts.

Abstract Objective To assess antiseizure effects of cenobamate, a new antiseizure medication with at least two mechanisms of action, in the rare, highly pharmacoresistant and progressive epilepsy syndrome related to Rasmussen's encephalitis. Methods Three patients from the epilepsy centers in Freiburg, Kork, and Valencia are reported with focal epilepsy which had been pharmacoresistant to more than 10 prior treatment regimens. Assessment included at least 1 year of follow‐up after cenobamate introduction and included seizure frequency, seizure severity (in particular status epilepticus) and changes in co‐medication. Results In the three patients, cenobamate add on treatment proved superior to all prior antiseizure and immunomodulatory treatments which had been individually applied. Not only were focal to bilateral tonic–clonic seizure completely controlled, but also focal motor status epilepticus no longer occurred. Co‐medication could be reduced in all patients. Significance This case series in a rare and highly pharmacoresistant epilepsy syndrome suggests high efficacy of cenobamate add‐on treatment for seizure control. This may be a valuable information in epilepsy related to Rasmussen encephalitis and calls for further elucidation of the mechanism involved in superior seizure control also compared to prior treatments including sodium channel blockers and benzodiazepines. Plain Language Summary Rasmussen's encephalitis is a rare type of epilepsy that gets worse over time and doesn't respond well to most seizure medications. We describe three patients who tried many treatments without much success, but when they added cenobamate to their treatment, it worked better than the other medications. This also let them lower the overall amount of medication they were taking.

Abstract Objective This study investigated the effectiveness and tolerability of brivaracetam (BRV) monotherapy in a large series of patients with epilepsy. Method This was a multicenter, retrospective, observational, non‐interventional study in 24 hospitals across Spain. Patients aged ≥18 years who started on BRV monotherapy, either as first‐line or following conversion, at least 1 year before database closure were included. Patients were evaluated at baseline and at 3, 6 and 12 months after initiation of BRV monotherapy, in accordance with usual clinical practice at these centers. Data were collected retrospectively from patients' individual charts by participating physicians. The primary effectiveness and safety endpoints were the percentage of seizure‐free patients 1 year after initiation of BRV monotherapy and the proportion of patients reporting adverse events (AEs) over the complete follow‐up period. Retention rates and subpopulation analysis (levetiracetam switchers, elderly and different etiologies) were also investigated. Results A total of 276 patients were included (48 with BRV as first‐line monotherapy and 228 who converted to BRV monotherapy). The overall retention rate in monotherapy at 12 months was 89.9% (87.5% for first‐line monotherapy group; 90.4% for conversion‐to‐monotherapy group). Seizure‐freedom rates at 12 months were 77.8% (75% for first‐line monotherapy group; 78.4% for conversion‐to‐monotherapy group). AEs occurred in 39.5% of patients at 12 months (35.4% for first‐line monotherapy group; 40.4% for conversion‐to‐monotherapy group). Most AEs were mild‐to‐moderate. The most frequent AEs were irritability (12.3%) and dizziness (10.1%). The most frequent AEs leading to BRV withdrawal were dizziness (1.8%) and memory problems (1.4%). Similar outcomes in terms of effectiveness and tolerability of BRV monotherapy were observed in patients switching from levetiracetam, those with different epilepsy etiologies, and elderly patients. Significance BRV was effective and well tolerated both as first‐line monotherapy and following conversion to monotherapy in a real‐world setting of patients with epilepsy. Plain Language Summary The goal of the medical treatment of epilepsy is to ensure best possible patient quality of life, by maximizing seizure control and minimizing medication toxicity. Brivaracetam (BRV) is a new‐generation epilepsy treatment that is well tolerated by patients. In our study, monotherapy with BRV reduced seizures in patients who had not received other treatments and in patients who switched from a previous treatment to BRV monotherapy. BRV was well tolerated and also effective in sensitive patients (i.e., the elderly and those who had epilepsy caused by a brain tumor or a brain injury).

Abstract Status epilepticus (SE) is a life‐threatening emergency with high morbidity and mortality. In people with epilepsy, the management of SE is focused on early medical treatment. Stiripentol is a third‐generation antiseizure medication (ASM) approved for refractory generalized tonic–clonic seizures in Dravet syndrome. The aim of this systematic review was to evaluate the effectiveness and safety of stiripentol in reducing the incidence of SE in patients with Dravet syndrome or any epilepsy characterized by recurrent SE. The PubMed and Cochrane databases were systematically searched, and gray literature was hand‐searched. Search results were screened by title and abstract; studies with data on the effect of stiripentol on SE outcomes, including the cessation of SE, reduction in number of SE episodes, or reduction in hospitalizations, were included. Of 66 records identified, 17 studies were eligible for inclusion, of which 15 were human studies (n = 474; aged 1.1–78 years), and two were animal experiments. Results of retrospective or prospective observational studies showed that stiripentol as add‐on therapy to ASMs such as clobazam or valproate reduced the incidence of SE in patients with Dravet syndrome or other developmental and epileptic encephalopathies (DEEs). A mean of 68% of patients (range 41%–100%) had a ≥50% reduction in SE episodes from baseline, and 26%–100% of patients (mean 77%) became SE‐free after stiripentol initiation. Moreover, this review found stiripentol, used as acute treatment, may also be effective for the cessation of super‐refractory SE, but data are limited to three retrospective case series. Stiripentol was generally well‐tolerated. In conclusion, stiripentol reduces the incidence of SE episodes in patients with Dravet syndrome and potentially other DEEs, and it promotes cessation of super‐refractory SE in patients with and without a history of seizures. Plain Language Summary Status epilepticus (SE) is a life‐threatening, long‐lasting seizure occurring in patients with/without epilepsy. This article analyzed 15 published studies that investigated the effects and safety of the anti‐seizure medication stiripentol for preventing SE in epilepsy patients (prevention) or stopping an SE episode (cessation), and two animal studies that investigated how stiripentol works. In epilepsy patients, stiripentol halved the number of SE episodes in 41–100% of patients, 26–100% of patients became SE‐free, and stiripentol was considered to be well tolerated. In patients with/without epilepsy, stiripentol may stop the SE episode after other drugs like anesthetics have not worked.

Abstract Avian photopic curves show energy peaks at different wavelengths than humans, particularly in the ultraviolet, yellow, and red range. Therefore, an illumination system with a spectrum tailored for laying hens, encompassing the entire visible spectrum, can enhance performance and welfare. The primary contribution of this study was the development of two Spectral Power Distributions (SPDs) specifically designed for laying hens, with different spectral proportions (S1 and S2), and the evaluation of their effects on productive performance, egg quality, and hormonal levels, compared to conventional white lighting at 3000 K (C). The SPD with a higher emission of red light to increased egg production and egg mass. It also had a lower melatonin concentration, suggesting an inverse relationship with the egg production rate. Regarding egg quality, SPDs specifically designed for laying hens resulted in eggs with greater weight (S1), shell strength (S2), and yolk height (S1 and S2) and diameter (S2). The study’s results indicate that lighting emitting wavelengths within the spectrum visible to poultry, with higher emissions at long wavelengths, appears to be more favorable for laying hens than conventional lighting. A spectrum with higher emissions at shorter wavelengths appears to impair the productive performance of laying hens.

Abstract The commercial importance of cephalopods has increased considerably, being an important fishing resource. However, during the preparation for commercialization of those species, they suffer the process known as “finning” which includes removing and separating the head, arm, skin or even having the body structure cut into rings, which ends up making it difficult or often prevents the identification of the species, which can lead to replacements. In this sense, the present study aimed to use the large ribosomal region, rrnL (16S rDNA) to genetically identify cephalopod species sold in markets and fairs in Latin America. Whole and processed samples were collected from supermarkets and directly from local fishers the approximate collection location. Each generated sequence was submitted to the blastn portal for molecular comparison and included in the database for subsequent genetic identification. Our results indicate labeling errors in samples from the State of Pará that contained the species Dosidicus gigas found only in the Pacific Ocean and were generically labeled as “National Lula”. No type of substitution was found among the samples that were being sold at fairs and markets, only labeling errors. Thus, our results demonstrate the effectiveness of the rrnL for identifying species and evaluating labeling errors.

PurposeTo determine the relationship between self-reported physical activity and the components of premorbid metabolic syndrome in patients treated in primary care according to sex.MethodsCross-sectional descriptive study conducted on a sample of 2,359 patients without cardiovascular disease or diabetes, included in the cohort of the IBERICAN study. Using ANOVA models and adjusting for age, economic status, employment situation, level of education, adherence to a Mediterranean diet, tobacco use and alcohol consumption, we estimated the association of the variables blood pressure, triglycerides, HDL cholesterol, blood glucose and waist circumference with the self-reported level of physical activity (sedentary, moderate, high, very high). The analyses were performed stratifying by sex.ResultsA total of 854 men and 1,505 women with no identified diseases were included. Women were more sedentary than men (pConclusionsPatients served in primary care clinics without diabetes or cardiovascular disease and with high levels of physical activity showed better metabolic syndrome profiles. Given that women are more sedentary, gender approaches are needed in the promotion of physical activity to prevent metabolic syndrome and cardiovascular disease.

Abstract Background Despite considerable evidence for the efficacy and safety of stiripentol in Dravet syndrome (DS), some aspects of stiripentol use remain challenging in clinical practice, such as dose titration and the adjustment of concomitant antiseizure medications (ASMs) to prevent potential adverse effects. Aim To (1) provide practical recommendations on the initiation of stiripentol treatment in patients with DS, (2) evaluate its effectiveness in the patient, and (3) guide the management of drug interactions and other aspects of treatment monitoring. Methods Six Spanish neurologists (the authors) with expertise in the management of pediatric and adult patients with DS held a meeting in early 2024 to develop expert recommendations regarding the use of stiripentol in DS, based on a review of the literature and their common clinical experience. Results According to these recommendations, stiripentol can be administered to patients with DS of any age, although its initiation and titration vary according to age group. Individualized adjustment of concomitant ASMs, such as valproic acid and clobazam or drugs specifically for DS (i.e., fenfluramine), at initiation and during stiripentol treatment, can mitigate drug interactions, thereby increasing the long-term tolerability of stiripentol treatment. In specific cases, stiripentol doses of > 50 mg/kg/day may be contemplated, and acute stiripentol administration may be considered to control refractory status epilepticus. Blood tests should be performed before starting stiripentol, at 3, 6, and 12 months after starting treatment, and then annually, except in the event of adverse effects, when additional testing may be necessary. Most adverse effects can be adequately managed by adjusting concomitant medications. Conclusion These practical recommendations may be easily adapted for use in different countries, and should increase physicians’ confidence in the initiation and monitoring of stiripentol treatment, thus facilitating effective management of patients with DS and improving clinical outcomes.

On the surface of the Cave of Altamira’s prehistoric paintings, a series of active deterioration processes are evident, leading to significant alterations of this invaluable heritage. This study proposes a comprehensive methodology for the systematic recording and management of these alterations. To achieve this, advanced microphotogrammetric monitoring techniques are employed, allowing for the acquisition of very high-resolution images that provide objective and quantifiable data that let us determine the evolution of the alterations. By comparing these images with those from earlier campaigns, the study tracks changes. The data collected through this protocol has helped with the development of new research avenues to understand, among the many alteration processes that impact paintings, the dynamics of water and fluid mechanics affecting the conservation of Cave of Altamira. These investigations help clarify how, why, and at what rate degradation processes such as pigment migration, washing, and bacterial colonization occur. The insights gained from these techniques inform indirect conservation measures aimed at reducing the deterioration of the cave art, located both on the Polychrome ceiling and throughout the rest of the Cave of Altamira. The results underline the importance of regular monitoring and the application of precise, non-invasive techniques to protect rock art from continued degradation. This research provides a model for similar conservation initiatives at other vulnerable heritage sites.

Abstract Patients with liver cirrhosis may show minimal hepatic encephalopathy (MHE) with motor incoordination which is reproduced in hyperammonemic rats. Hyperammonemia induces peripheral inflammation which triggers neuroinflammation and enhanced GABAergic neurotransmission in cerebellum and motor incoordination. The mechanisms involved remain unknown. The aims were to assess if the early increase of peripheral IL-17 triggers motor incoordination in hyperammonemic rats and to identify some underlying mechanisms. We assessed if blocking peripheral IL-17 with anti-IL-17 at 2–4 days of hyperammonemia prevents motor incoordination and analyzed underlying mechanisms. Hyperammonemia induces a transient blood IL-17 increase at days 3–4. This is associated with increased IL-17 receptor membrane expression and activation in cerebellum, leading to NADPH oxidase activation, increased superoxide production and MLCK that induce blood–brain barrier (BBB) permeabilization by reducing occludin and ZO-1. BBB permeabilization facilitates the entry of IL-17, which increases in cerebellum and activates microglia. This increases TNFα and the TNFR1-S1PR2-CCL2-BDNF-TrkB pathway. This enhances GABAergic neurotransmission which impairs motor coordination. Blocking peripheral IL-17 with anti-IL-17 prevents all the above process and prevents motor incoordination. Early treatment to reduce blood IL-17 may be a useful treatment to reverse motor incoordination in patients with MHE. Graphical abstract

Abstract The redirection of T lymphocytes against tumor-associated or tumor-specific antigens, using bispecific antibodies or chimeric antigen receptors (CAR), has shown therapeutic success against certain hematological malignancies. However, this strategy has not been effective against solid tumors. Here, we describe the development of CAR T cells targeting p95HER2, a tumor-specific antigen found in HER2-amplified solid tumors. These CAR T cells display robust activity against p95HER2-expressing cell lines but demonstrate limited efficacy against patient-derived xenografts. As p95HER2 is invariably detectable on tumor cells that overexpress HER2, but not those that express HER2 at normal levels, we arm p95HER2-specific CAR T cells with affinity-tuned bispecific antibodies against HER2 and CD3 in order to redirect them only to HER2-amplified cells. The combination of p95HER2.CAR T cells and HER2 x CD3 bispecific antibodies lead to a complete regression in three HER2-positive, patient-derived mouse xenografts tumor models. This combination represents a promising strategy to redirect T cells against a subset of HER2-positive tumors.

Abstract The crystalline lens of the human eye possesses a distinct convex shape, a subject that has garnered significant attention in optical research. In our study, we delve into the methodologies for quantifying the convexity of these lenses. Utilizing lens maps as our foundational model, we demonstrate that these mappings exhibit convexity of order β > 0 $\beta >0$ and are starlike of order α > 0 $\alpha >0$ . Furthermore, we establish a direct correlation between these orders and the opening angle of the lens map.

Abstract Background The herd age structure, i.e., distribution of sows within a farm based on their parity number, and its management are essential to optimizing farm reproductive efficiency. The objective of this study is to define different types of herd age structure using data from 623 Spanish commercial sow farms. Additionally, this study aims to determine which type of herd age structure can enhance reproductive efficiency at the farm level. Results Farms are classified into three groups according to the quadratic function fitted to the percentage of sows by parities. This classification unveils three types of herd structures: type 1 (HS1) exhibits a concave-downward trend, with a higher percentage of sows in intermediate parities (mean of 45.5% sows between the 3rd to 5th parity); type 2 (HS2) presents a trend curve that is close to a straight line, with a gradual decrease in the percentage of sows per parity (approximately 2% loss of sows census per parity); and type 3 (HS3) shows an upward concave trend curve, with an increase in the percentage of sows in later parities (19.0% of sows between 7th and ≥ 8th parity). Parametric tests assess productivity differences between the three types of herd structures (p

Abstract Machine learning for financial risk prediction has garnered substantial interest in recent decades. However, the class imbalance problem and the dilemma of accuracy gain by loss interpretability have yet to be widely studied. Symbolic classifiers have emerged as a promising solution for forecasting banking failures and estimating creditworthiness as it addresses class imbalance while maintaining both accuracy and interpretability. This paper aims to evaluate the effectiveness of REMED, a symbolic classifier, in the context of financial risk management, and focuses on its ability to handle class imbalance and provide interpretable decision rules. Through empirical analysis of a real-world imbalanced financial dataset from the Federal Deposit Insurance Corporation, we demonstrate that REMED effectively handles class imbalance, improving performance accuracy metrics while ensuring interpretability through a concise and easily understandable rule system. A comparative analysis is conducted against two well-known rule-generating approaches, J48 and JRip. The findings suggest that, with further development and validation, REMED can be implemented as a competitive approach to improve predictive accuracy on imbalanced financial datasets without compromising model interpretability.

Abstract Metabolic syndrome has a multifactorial origin; however, epidemiological data correspond to populations located at sea level. It has been reported that the altitude can affected the prevalence due to physiological changes. The aim of this study is to show the global prevalence of metabolic syndrome at altitude and its components. We use four databases, all studies published up to November 2023. The prevalences from studies were meta-analyzed using a random-effects model. To assess sources of heterogeneity, subgroup analyses were performed. We included 28 studies. The number of participants was 29 195. The prevalence of metabolic syndrome was 30.3% (95% CI 22.8–38.4%). According to the altitude level, at 1500–2500 was 36.5%, 2500–3500 (21.8%), and > 3500 (30.9%), also it was higher in women (35.5%) that men (26.8%). It was observed that there is an inverse relationship between higher altitude and the prevalence of metabolic syndrome. Among its components, abdominal obesity and low HDL were present in more than 40.0%, while high blood pressure, high triglycerides and impaired glucose were present in less than 30.0%. We recommend that our results be considered for future research in populations living at altitude since they have different characteristics from populations at sea level.

Abstract Congenital heart disease (CHD) is the most common birth defect in newborns, often requiring cardiac surgery with concomitant thymectomy that is known to increase disease susceptibility later in life. Studies of γδ T cells, which are one of the dominant T cells in the early fetal human thymus, are rare. Here, we provide a comprehensive analysis of the γδ T cell compartment via flow cytometry and next-generation sequencing in children and infants with CHD, who underwent cardiac surgery shortly after birth. A perturbation of the γδ T cell repertoire is evident, and Vδ1 T cell numbers are reduced. However, those cells that are present, do retain cytotoxicity. In contrast, GZMA+CD28+CD161hi innate effector Vγ9Vδ2 T cells are found in higher proportions. TCR-seq identifies an increase in TRDJ3+ γδ T cell clones in children with CHD, but not in a confirmatory group of neonates prior to CHD surgery, which overall points to a persistence of fetal-derived effector γδ T cells in children with CHD.

Abstract Background Despite its proven prognostic value in different contexts, the precise implications of left atrial strain (LAS) assessment throughout different phases of the atrial cycle remain uncertain. A direct correlation between left atrial reservoir strain (LARS) and left ventricular global longitudinal strain (GLS) has been consistently demonstrated in several studies involving patients with various heart diseases. The objective of our study is to identify factors directly associated with LARS, left atrial conduction strain (LACS) and left atrial booster strain (LABS) in patients without cardiovascular (CV) disease. Methods Transthoracic echocardiographic examinations in patients without CV disease were prospectively selected in two tertiary hospitals echocardiography labs for clinical purposes. LAS, maximal and minimal left atrial (LA) volumes and left atrial ejection fraction (LAEF) were measured using the two-dimensional strain analysis package provided by the EchoPAC Plugging workstation (AFI LA). Results A total of 196 cases were included, median age of 54 (45–62) with 85 (43%) being men. The mean left ventricular ejection fraction (LVEF) was 61% ± 5, and the median GLS was − 18% (-17 to -20). Median indexed maximum volume of left atrium (LAVI) was 27 ml/m2 (22–31), and LAEF was 64% (58–70). The mean LARS biplane was 35,1% ± 8. Notably, LARS was greater in the 2-chamber view (36,1% ± 10) compared to the 4-chamber view (34,1% ± 8 p

Abstract During COVID-19 pandemic, international pharmaceutical companies put effort to build global manufacturing networks for vaccines. Soberana Plus vaccine, a recombinant protein based vaccine (RBD dimer), with the trade name of PastoCovac Plus in Iran, is based on a protein subunit platform in Cuba and completed preclinical and toxicological assessments. This study aimed at presenting the steps of vaccine technology transfer from Cuba to Iran. This study provides the first practical comparability results in Iran to ensure the quality, safety and efficacy of a protein subunit vaccine against COVID-19 after a successful technology transfer from Cuba. PastoCovac Plus was transferred to Iran at the formulation stage. The assessment of the active ingredient pharmaceutical (API) was achieved through physicochemical and clinical data collection and tests to assure if there was any adverse impact on the vaccination results. In order to assess the quality of the vaccine product after technology transfer, we sought different properties including regulatory features, physicochemical quality, vaccine potency and stability as well as its immunogenicity and safety. Following the evaluation of the clinical quality attributes (CQAs) based on the standard protocols, the results showed that the two vaccines are highly similar and comparable, with no considerable effect on safety or efficacy profiles. The CQAs were all in the acceptance limits in terms of safety and efficacy as well as clinical evaluation results. The immunogenicity evaluation also confirmed no significant differences between the vaccines regarding reinfection (P = 0.199) or vaccine breakthrough (P = 0.176). Furthermore, the level of anti-spike and neutralizing antibodies in the both vaccine groups was not significantly different indicating the equality of performance between the two vaccines. According to the results of the quality and clinical assessment of this study, we achieved an acceptable quality attributes and acceptant limits in terms of safety and efficacy of the vaccines pre and post technology transfer.

Dielectric resonator antennas have been demonstrated to be an efficient radiator, especially with the possibility of fabricating it at low cost based on 3-D printing technologies. However, they have two main limitations which are: their relatively small gain (about 5dBi) and narrow bandwidth. To enhance the gain of DRA, higher-order mode excitation can be utilized; however, typically this has a limit of a few dB increments besides its negative effects on the bandwidth. This study employs a cavity-backed slot to provide an appropriate feeding method for a cylindrical dielectric resonator antenna (CDRA). This design effectively facilitates the excitation of higher-order modes, resulting in a significant gain of up to 15 dBi, which is essential for many wireless systems that are unable to support larger aperture sizes through the standard use of arrays. Additionally, two modes are excited to keep a relatively broadband of about 15 %. The proposed design consists of a cylindrical dielectric placed on top of a cavity-backed slot that is excited by a coaxial probe soldered to the top patch of the cavity. With this feeding, a good matching is achieved for different dielectric resonator heights, hence, the gain can be adjusted easily without the need of reoptimizing the antenna or the feed structures. The design process is explained in addition to performing a sensitivity analysis of fabrication tolerances which confirms that the 3-D printer provides enough accuracy with a negligible effect on the antenna’s performance. Then a prototype with a medium length is fabricated and measured to provide high realized gain and impedance bandwidth of 11 dBi and 14.5 %, respectively. The measured data demonstrate the good performance of the antenna with great matching compared to the simulated ones. Furthermore, the antenna presents a good performance in terms of sidelobes and polarization. This proposed design outperforms other DRA designs based on higher-order modes.

Purpose: Our objective was to synthesize and determine whether there are sex differences in physical function following exercise interventions in older adults. Materials and Methods: A systematic search was conducted in four databases from inception to July 8th, 2023 searching for prospective trials that conducted exercise interventions in older adults and results for physical function were reported by sex. Pooled standardized mean differences (SMDs) with their 95% confidence intervals (CIs) were estimated using a randomeffects method. The Sidik-Jonkman estimator was used to calculate the variance of heterogeneity (I2). Results: A total of 19 studies involving 20,133 older adults (mean age ≥60 years, 33.7% female) were included. After exercise interventions, males reported significantly greater pre-post changes compared to females for upper body strength (SMD=- 0.40, 95% CI: -0.71 to -0.09; I2=75.6%; n=8), lower body strength (SMD=-0.32, 95% CI: -0.55 to -0.10; I2=52.0%; n=11), and cardiorespiratory fitness (SMD=-0.29, 95% CI: -0.48 to -0.10; I2=89.1%; n=12). Conversely, the pooled SMDs showed a significant effect favoring females for motor fitness (SMD=0.21, 95% CI: 0.03 to 0.39; I2=0%; n=7). Limited and inconsistent results were observed for flexibility. Conclusions: Our study suggests the existence of sex-related differences on physical function after an exercise intervention in the older population.

Abstract Background Sarcopenia and low areal bone mineral density (aBMD) are prevalent musculoskeletal complications after paediatric cancer treatment. However, their relationship has not been examined in young paediatric cancers survivors. This study aimed to evaluate aBMD differences according to sarcopenia status and the risk of low aBMD Z‐score in young paediatric cancer survivors with sarcopenia confirmed/probable. Methods This cross‐sectional study included 116 paediatric cancer survivors (12.1 ± 3.3 years old; 42.2% female). Handgrip strength was used to assessed muscle strength. Dual‐energy X‐ray absorptiometry estimated aBMD (g/cm2) and appendicular lean mass index (ALMI, kg/m2). ‘No sarcopenia’ was defined when muscle strength was >decile 2. ‘Sarcopenia probable’ was defined when muscle strength was ≤ decile 2 and ALMI Z‐score was > −1.5 standard deviation (SD). ‘Sarcopenia confirmed’ was defined when muscle strength was ≤ decile 2 and ALMI Z‐score ≤ −1.5 SD. Analysis of covariance and logistic regression, adjusted for time from treatment completion, radiotherapy exposure, calcium intake, and physical activity, was used to evaluate aBMD and estimate the odds ratios (ORs) of low aBMD (aBMD Z‐score < −1.0). Results Survivors with sarcopenia confirmed had significantly lower aBMD than those without sarcopenia at total body (−1.2 [95% CI: −1.5 to −0.8] vs. 0.2 [−0.2 to 0.6], P

Abstract Background Mexico reports low follow-up completion rates among women with abnormal cervical cancer screenings. This study aimed to identify barriers and facilitators to follow-up adherence among women with human papillomavirus (HPV) infection and premalignant cervical lesions in Mexico. Methods A mixed-methods study was conducted from February to April 2019. Participants included women undergoing follow-up care for high-risk human papillomavirus (HR-HPV) and premalignant lesions, along with health personnel from the Women’s Healthcare Center (CAPASAM) in Mexico. Quantitative data were obtained from the Women’s Cancer Information System and through a questionnaire about factors affecting follow-up adherence. Additionally, the health personnel involved completed a compliance checklist regarding care regulations. Descriptive statistics were used for analysis. Qualitative data were collected via semi-structured interviews with both groups, followed by a content analysis based on identified categories. The Hazard Analysis and Critical Control Point System confirmed care process risks. Proposals to enhance the Early Detection Program for Prevention and Control of Cervical Cancer were collected from a CAPASAM health personnel nominal group. Results Identified barriers to follow-up included low income among CAPASAM users, family provider roles limiting time for appointments, long waits for testing and results delivery, distant facilities, insufficient service hour communication, inadequate health personnel training, and a lack of systematic counseling. Hesitation toward follow-up was also linked to shame, apprehension, uncertainty, test aversion, fear of positive results, and limited cervical cancer and screening knowledge. Patriarchal attitudes of partners and limited access to the now-discontinued PROSPERA government program further discouraged follow-up. Facilitators comprised respectful treatment by CAPASAM staff, no-cost services, health campaigns, and positive user attitudes. Conclusions The study found more barriers than facilitators to follow-up adherence, highlighting the need for strategies to bolster the Early Detection Program. Future strategies must address the comprehensive array of factors and incorporate stakeholder perspectives.

With the advent of modern Medicine, the connections between emotions and medical conditions have primarily been described through observational studies, where conclusions are drawn from correlations of observations rather than experimental research. Psychoanalytical theory has often been used to explain many common illnesses. This approach remains prevalent today. Interestingly, despite the wealth of information now available on the influence of emotions on health, the current divide between neurology and psychiatry, as well as the separation between mental health services and primary care, combined with the time constraints and physicians’ workload, have led to a fragmented approach to many disorders. In these cases, psychological and emotional factors play an important role but are often inadequately addressed. The objective of this paper is to analyse the existing evidence on the relationship between anger and health through a narrative review. The evidence and data presented point to the need for analytical studies based on scientific methodology to explore the relationship between anger and health, and particularly to adopt an integral health approach taking into account social determinants and the role of empathy among the healthcare professionals and clinical and social care. The prevention and treatment of problems related to the high prevalence and intensity of anger are particularly relevant, given the potential negative impact this emotion can have on patients’ physical and mental health, as well as their social adjustment and relationships.

Abstract Background Type 2 diabetes mellitus (T2DM) is a major risk factor for heart failure with preserved ejection fraction and cardiac arrhythmias. Precursors of these complications, such as diabetic cardiomyopathy, remain incompletely understood and underdiagnosed. Detection of early signs of cardiac deterioration in T2DM patients is critical for prevention. Our goal is to quantify T2DM-driven abnormalities in ECG and cardiac imaging biomarkers leading to cardiovascular disease. Methods We quantified ECG and cardiac magnetic resonance imaging biomarkers in two matched cohorts of 1781 UK Biobank participants, with and without T2DM, and no diagnosed cardiovascular disease at the time of assessment. We performed a pair-matched cross-sectional study to compare cardiac biomarkers in both cohorts, and examined the association between T2DM and these biomarkers. We built multivariate multiple linear regression models sequentially adjusted for socio-demographic, lifestyle, and clinical covariates. Results Participants with T2DM had a higher resting heart rate (66 vs. 61 beats per minute, p

Abstract Juvenile‐onset Huntington's disease (HD) is a rare subset of HD with symptom‐onset before the age of 18. In contrast to the adult population, children present early‐on with behavioral, psychiatric, and cognitive symptoms, in addition to a diverse spectrum of movement disorders. This poses a distinct challenge in diagnosis and management. We here describe the spectrum of movement disorders, accompanied with detailed video recordings, in seven cases of juvenile‐onset HD. Our findings highlight early cognitive and behavioral symptoms, preceding motor symptoms. The diverse movement disorder phenotypes included dystonia, Parkinsonism, myoclonus, and chorea, findings which underscore the heterogeneity of presenting symptoms.

Abstract Titanium alloys are among the widely used biomedical materials due to their biocompatibility and mechanical performance. It is an indisputable fact that the materials used in this area must be antibacterial. Therefore, in this study, the production of antibacterial Titanium alloys using NanoAg particles for use in biomedical applications was investigated. First, Ti-6Al-4 V titanium alloys were produced by the powder metallurgy method, which includes blending, pressing and sintering processes. Nano silver particles were synthesized by a chemical reduction method using silver nitrate and glucose. The resulting solution was subjected to Ag + ions and glucose determinations, pH and turbidity analysis. Nano Ag application was carried out by two different methods; dipping and coating method. The obtained samples were analyzed by scanning electron microscopy (SEM) and optical microscopy. Additionally, as the main purpose of this study, antibacterial analysis against Escherichia Coli and Staphylococcus Aureus was performed by percentage reduction test. While the coating method was seen to be more successful in antibacterial tests, the overall Nano Ag application performance was found to be higher against Escherichia Coli bacteria. Being that the sample containing 5% Nano Ag titanium alloys produced with the coating technique showed 100% killing effect against both Staphylococcus aureus and Escherichia coli.

Introduction: Creatine (Cr) is an effective and safe ergogenic supplement that enhances post-exercise recovery, prevents injuries, supports thermoregulation, and provides benefits in rehabilitation, as well as spinal and cerebral neuroprotection for adults and older individuals. However, the evidence regarding its benefits in the pediatric population across various contexts has not been conclusive. Objective: Analyze the impact of different doses of Cr on physical performance and muscle function in children and adolescents in general through a systematic review of the literature. Materials and methods: This review followed the PRISMA reporting guidelines in the electronic databases of PubMed/Medline and Google Scholar. It included controlled clinical trials conducted between 1997 and May 2023 that assessed the effect of Cr supplementation on muscle function and physical performance in children and adolescents. A total of 20 studies were included. Results: The subjects included young high-performance athletes from different disciplines and children or adolescents diagnosed with pathologies affecting muscle function. Significant effects were found in athletes' physical performance and muscular function at various doses and periods of supplementation, unlike those involving a pathology, which mainly did not present improvements in these variables. Conclusions: Some reports suggest a positive effect on physical performance, specifically in young athletes. However, the Cr doses, posology, and administration protocols vary between studies.

The study analyzes whether the type of shareholding control (dispersed, shared, or dominant) affects agency conflicts by investigating the relationship between shareholding control, ownership concentration, and firm value. The sample is a panel data comprising 1977 firm-year observations from 167 Brazilian firms in the period 2010–2022. Our results show that shared control creates value, whereas dominant control destroys firm value. Voting rights concentration and cash-flow rights reduce the value of firms with dispersed and shared control. However, at firms with dominant control, these firm characteristics increase firm value. Excess voting rights, in turn, destroy the value of firms with shared and dominant control. Our evidence shows that the type of shareholding control influences the nature and magnitude of agency conflicts and the relationship between ownership concentration and firm value. Thus, within an institutional environment, agency conflicts may differ among firms, depending on the type of shareholding control.

Abstract A key aspect of sustainable bioeconomy is the recirculation of renewable, agricultural waste streams as substrates for microbial production of high-value compounds. One approach is the bioconversion of corn stover, an abundant maize crop byproduct, using the fungal maize pathogen Ustilago maydis. U. maydis is already used as a unicellular biocatalyst in the production of several industrially-relevant compounds using plant biomass hydrolysates. In this study, we demonstrate that U. maydis can grow using untreated corn stover as its sole carbon source. We developed a small-scale bioreactor platform to investigate U. maydis processing of corn stover, combining online monitoring of fungal growth and metabolic activity profiles with biochemical analyses of the pre- and post-fermentation residues. Our results reveal that U. maydis primarily utilizes soluble sugars i.e., glucose, sucrose and fructose present in corn stover, with only limited exploitation of the abundant lignocellulosic carbohydrates. Thus, we further explored the biotechnological potential of enhancing U. maydis´ lignocellulosic utilization. Additive performance improvements of up to 120 % were achieved when using a maize mutant with increased biomass digestibility, co-fermentation with a commercial cellulolytic enzyme cocktail, and exploiting engineered fungal strains expressing diverse lignocellulose-degrading enzymes. This work represents a key step towards scaling up the production of sustainable compounds from corn stover using U. maydis and provides a tool for the detailed monitoring of the fungal processing of plant biomass substrates. Graphical abstract

Abstract Background Cardiovascular disease is a major cause of morbidity in an aging HIV population. However, risk estimation with the most frequent equations usually classifies HIV patients as having a low or moderate risk. Several studies have described a very high prevalence of subclinical atherosclerosis in a middle-aged, non-HIV population. There is insufficient body of knowledge to understand if this is the case in people living with HIV (PLWH). We aim to calculate the proportion of patients with subclinical atherosclerosis in a single site cohort of HIV-infected subjects. Methods We have analyzed chronically HIV infected adults (≥ 18 years) who were on active follow-up in an HIV unit specialized in the care of cardiovascular health. The most recent clinical visit and vascular ultrasonography were used to assess the objectives of our research. Our primary objective was to describe the proportion of participants with subclinical atherosclerosis (focal protrusion into the lumen > 0.5 mm or > 50% of the surrounding IMT or a diffuse thickness > 1.5 mm) in a single site cohort of PLWH. Carotid and iliofemoral territories were evaluated. As a secondary objective we have run a multivariate analysis to determine which HIV and non-HIV factors might be related with the presence of atherosclerotic plaques. Findings We included a total of 463 participants between November 2017 to October 2019. Subjects were predominantly male (84.2%) with a mean age of 48.8 years (SD 10.7). Hypercholesterolemia (36%) was the most prevalent comorbidity followed by Hypertension (18%) and Hypertriglyceridemia (16%). Mean duration of HIV infection is 12.3 years. Overall, participants had been receiving cART for a median of 9.5 years. Subclinical atherosclerosis was found in 197 subjects (42.5%; CI 95% [38.0–47.2]). The disease was found more frequently in the femoral arteries (37.8%) than in the carotid vascular bed (18.6%). Despite some HIV factors correlated with the presence of plaques in a univariate analysis (e.g., time with HIV-1 RNA > 50 copies/mL or time from HIV diagnosis), the only two explanatory factors that remained associated with the presence of atherosclerotic plaques in the multivariate analysis were smoking (OR 5.47, 95% CI 3.36 – 8.90) and age (OR 1.13, 95%CI 1.10 – 1.16). Interpretation We have found a very high prevalence of subclinical atherosclerosis among our cohort of PLWH. Despite having analyzed several HIV factors, age and smoking have been found to be the only factors associated with the development of atherosclerotic plaques.

Abstract Advances in omics technologies have enabled the in-depth study of microbial communities and their metabolic profiles from all environments. Here metagenomes were sampled from piranha (Serrasalmus rhombeus) and from river water from the Rio São Benedito (Amazon Basin). Shotgun metagenome sequencing was used to explore diversity and to test whether fish microbiomes are a good proxy for river microbiome studies. The results showed that the fish microbiomes were not significantly different from the river water microbiomes at higher taxonomic ranks. However, at the genus level, fish microbiome alpha diversity decreased, and beta diversity increased. This result repeated for functional gene abundances associated with specific metabolic categories (SEED level 3). A clear delineation between water and fish was seen for beta diversity. The piranha microbiome provides a good and representative subset of its river water microbiome. Variations seen in beta biodiversity were expected and can be explained by temporal variations in the fish microbiome in response to stronger selective forces on its biodiversity. Metagenome assembled genomes construction was better from the fish samples. This study has revealed that the microbiome of a piranha tells us a lot about its river water microbiome and function.

Abstract Background Parasitic neglected tropical diseases (NTDs) or ‘infectious diseases of poverty’ continue to affect the poorest communities in the world, including in the Philippines. Socio-economic conditions contribute to persisting endemicity of these infectious diseases. As such, examining these underlying factors may help identify gaps in implementation of control programs. This study aimed to determine the prevalence of schistosomiasis and soil-transmitted helminthiasis (STH) and investigate the role of socio-economic and risk factors in the persistence of these diseases in endemic communities in the Philippines. Methods This cross-sectional study involving a total of 1,152 individuals from 386 randomly-selected households was conducted in eight municipalities in Mindanao, the Philippines. Participants were asked to submit fecal samples which were processed using the Kato-Katz technique to check for intestinal helminthiases. Moreover, each household head participated in a questionnaire survey investigating household conditions and knowledge, attitude, and practices related to intestinal helminthiases. Associations between questionnaire responses and intestinal helminth infection were assessed. Results Results demonstrated an overall schistosomiasis prevalence of 5.7% and soil-transmitted helminthiasis prevalence of 18.8% in the study population. Further, the household questionnaire revealed high awareness of intestinal helminthiases, but lower understanding of routes of transmission. Potentially risky behaviors such as walking outside barefoot and bathing in rivers were common. There was a strong association between municipality and prevalence of helminth infection. Educational attainment and higher “practice” scores (relating to practices which are effective in controlling intestinal helminths) were inversely associated with soil-transmitted helminth infection. Conclusion Results of the study showed remaining high endemicity of intestinal helminthiases in the area despite ongoing control programs. Poor socio-economic conditions and low awareness about how intestinal helminthiases are transmitted may be among the factors hindering success of intestinal helminth control programs in the provinces of Agusan del Sur and Surigao del Norte. Addressing these sustainability gaps could contribute to the success of alleviating the burden of intestinal helminthiases in endemic areas.