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Your search keyword '"Van der Hoeven, Gerd"' showing total 11 results
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11 results on '"Van der Hoeven, Gerd"'

6. De Novo Variants in LMNB1 Cause Pronounced Syndromic Microcephaly and Disruption of Nuclear Envelope Integrity

Author

Cristofoli, Francesca, Moss, Tonya, Moore, Hannah W., Devriendt, Koen, Flanagan-Steet, Heather, May, Melanie, Jones, Julie, Roelens, Filip, Fons, Carmen, Fernandez, Anna, Martorell, Loreto, Selicorni, Angelo, Maitz, Silvia, Vitiello, Giuseppina, Van der Hoeven, Gerd, Skinner, Steven A., Bollen, Mathieu, Vermeesch, Joris R., Steet, Richard, and Van Esch, Hilde

Published
2020
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9. Hematopoietic Sphingosine 1-Phosphate Lyase Deficiency Decreases Atherosclerotic Lesion Development in LDL-Receptor Deficient Mice

Author

Bot, Martine, primary, Van Veldhoven, Paul P., additional, de Jager, Saskia C. A., additional, Johnson, Jason, additional, Nijstad, Niels, additional, Van Santbrink, Peter J., additional, Westra, Marijke M., additional, Van Der Hoeven, Gerd, additional, Gijbels, Marion J., additional, Müller-Tidow, Carsten, additional, Varga, Georg, additional, Tietge, Uwe J. F., additional, Kuiper, Johan, additional, Van Berkel, Theo J. C., additional, Nofer, Jerzy-Roch, additional, Bot, Ilze, additional, and Biessen, Erik A. L., additional

Published
2013
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11. A Nonradioactive Fluorimetric SPE-Based Ceramide Kinase Assay Using NBD-C6-Ceramide.

Author

Van Overloop, Helena, Van der Hoeven, Gerd, and Van Veldhoven, Paul P.

Subjects
*CERAMIDES, *FLUORIMETRY, *SOLID phase epitaxial growth, *FENRETINIDE, *FLUORESCENCE, *RADIOACTIVE substances, *IN vitro studies, *THERAPEUTICS
Abstract

Ceramide kinase (CERK) has been implicated in important cellular processes such as inflammation and apoptosis. Its activity is usually measured using radiolabeled ceramide or [γ-32P]-ATP, followed by extraction, thin-layer chromatography, and detection of the formed labeled ceramide-1-phosphate. To eliminate the use of radioactivity, we developed similarly but independently from the approach by Don and Rosen (2008), a fluorescence-based ceramide kinase assay, using N-[7-(4-nitrobenz-2-oxa-1,3- diazole)]-6-aminohexanoyl-sphingenine (NBD-C6-ceramide) as substrate. Its Km value (4 μM) was comparable to that of Nhexanoyl- sphingenine (C6-ceramide). The produced fluorescent NBD-C6-ceramide-1-phosphate was captured by means of solidphase extraction on an aminopropyl phase, resulting in a fast and sensitive CERK measurement. By performing this assay in a 96-well format, it is also suitable for high-throughput screening (HTS) to search for CERK modulators. A limited screen revealed that some protein kinase inhibitors (e.g., U-0126; IC50 4 μM) and ceramide analogues (e.g., fenretinide, AMG-9810; IC50 1.1 μM) affect CERK in vitro. [ABSTRACT FROM AUTHOR]

Published
2012
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