Search

Your search keyword '"Université libre de Bruxelles (ULB)"' showing total 5,602 results
Start Over Author "Université libre de Bruxelles (ULB)" Search Limiters Available in Library Collection
5,602 results on '"Université libre de Bruxelles (ULB)"'

1. A statistical analysis of time trends in atmospheric ethane

Author

Friedrich, Marina, Beutner, Eric, Reuvers, Hanno, Smeekes, Stephan, Urbain, Jean-Pierre, Bader, Whitney, Franco, Bruno, Lejeune, Bernard, Mahieu, Emmanuel, Department of Econometrics and Data Science, Vrije Universiteit Amsterdam, 1081HV Amsterdam, The Netherlands, Department of Econometrics, Erasmus University, 3062PA Rotterdam, The Netherlands, Department of Quantitative Economics, Maastricht University, 6200MD Maastricht, The Netherlands, Agence Wallone de l’Air et du Climat, Avenue Prince de Liège, Jambes, Belgium, Spectroscopy, Quantum Chemistry and Atmospheric Remote Sensing (SQUARES), Université libre de Bruxelles (ULB), Brussels, Belgium, Institute of Astrophysics and Geophysics, University of Liège, Liège, Belgium, Econometrics, Econometrics and Data Science, QE Econometrics, RS: GSBE Theme Data-Driven Decision-Making, and RS: FSE DACS Mathematics Centre Maastricht

Subjects
FOS: Computer and information sciences, Atmospheric Science, Heteroscedasticity, 010504 meteorology & atmospheric sciences, Econometrics (econ.EM), BANDWIDTH, Atmospheric sciences, Statistics - Applications, 01 natural sciences, INCREASE, TROPOSPHERE, FOS: Economics and business, Environnement et pollution, Troposphere, 010104 statistics & probability, chemistry.chemical_compound, Break point estimation, Trend analysis, BOOTSTRAP, Phénomènes atmosphériques, REGRESSION, SDG 13 - Climate Action, SPECTRA, Applications (stat.AP), Tropospheric ozone, 0101 mathematics, Economics - Econometrics, 0105 earth and related environmental sciences, Global and Planetary Change, SPECTROSCOPY, Autocorrelation, SERIES, Atmospheric ethane, ddc:551.5, Bootstrapping (electronics), Autoregressive model, chemistry, 13. Climate action, Greenhouse gas, TESTS, Environmental science, Bootstrapping, BURDEN
Abstract

Ethane is the most abundant non-methane hydrocarbon in the Earth’s atmosphere and an important precursor of tropospheric ozone through various chemical pathways. Ethane is also an indirect greenhouse gas (global warming potential), influencing the atmospheric lifetime of methane through the consumption of the hydroxyl radical (OH). Understanding the development of trends and identifying trend reversals in atmospheric ethane is therefore crucial. Our dataset consists of four series of daily ethane columns. As with many other decadal time series, our data are characterized by autocorrelation, heteroskedasticity, and seasonal effects. Additionally, missing observations due to instrument failure or unfavorable measurement conditions are common in such series. The goal of this paper is therefore to analyze trends in atmospheric ethane with statistical tools that correctly address these data features. We present selected methods designed for the analysis of time trends and trend reversals. We consider bootstrap inference on broken linear trends and smoothly varying nonlinear trends. In particular, for the broken trend model, we propose a bootstrap method for inference on the break location and the corresponding changes in slope. For the smooth trend model, we construct simultaneous confidence bands around the nonparametrically estimated trend. Our autoregressive wild bootstrap approach, combined with a seasonal filter, is able to handle all issues mentioned above (we provide R code for all proposed methods on https://www.stephansmeekes.nl/code.)., Horizon 2020 https://doi.org/10.13039/501100007601, Fonds De La Recherche Scientifique - FNRS https://doi.org/10.13039/501100002661

Published
2020

2. Les droits intellectuels, entre autres droits fondamentaux :La Cour de justice à la recherche d’un « juste équilibre » en droit d’auteur

Author

Conférence Les droits intellectuels, entre autres droits :intersections, interactions et interrogations organisée par J. CABAY et A. STROWEL dans le cycle UB3 organisé en partenariat entre l’Université Libre de Bruxelles (ULB), l’Université Saint-Louis – Bruxelles (USL-B) et le Barreau de Bruxelles (16 décembre 2019: Bruxelles, Université Saint-Louis – Bruxelles (USL-B)), Cabay, Julien, Lambrecht, Maxime, Conférence Les droits intellectuels, entre autres droits :intersections, interactions et interrogations organisée par J. CABAY et A. STROWEL dans le cycle UB3 organisé en partenariat entre l’Université Libre de Bruxelles (ULB), l’Université Saint-Louis – Bruxelles (USL-B) et le Barreau de Bruxelles (16 décembre 2019: Bruxelles, Université Saint-Louis – Bruxelles (USL-B)), Cabay, Julien, and Lambrecht, Maxime

Abstract

info:eu-repo/semantics/nonPublished

Published
2019

3. Role of curvature sensing/inducing BAR domain proteins in clathrin-independent endocytosis

Author

Renard, Henri-François, Seminar of the \\'Institut de Biologie et de Médecine Moléculaires\\' (IBMM) from the \\'Université Libre de Bruxelles\\' (ULB), and UCL - SST/ISV - Institut des sciences de la vie

Abstract

Endocytosis is an essential cellular process required for uptake of nutrients from cell environment and turnover of plasma membrane components. Clathrin-mediated endocytosis is by far the best characterized endocytic process. Since the mid-90's, the existence of endocytic routes independent of clathrin emerged in mammalian cells. Therefore, the most challenging question in membrane biology rose: how can the plasma membrane be deformed in the absence of an organized clathrin coat? Until today, this question has not been fully solved. First attempts to shed light into this topic demonstrated the requirement of glycosphingolipids for membrane deformation in lectin-driven endocytosis (for instance, the endocytosis of the bacterial lectin Shiga toxin). Recently, BAR domain proteins (where BAR stands for Bin/Amphiphysin/Rvs) have been described as crucial for clathrin-independent endocytic routes. BAR domain proteins interact with membranes and act as curvature sensors/inducers. The function of many BAR domain proteins remains unclear in the landscape of clathrin-independent endocytosis, and especially regarding their role in the endocytic pit formation. The main part of my talk will be focused on the implication of a specific BAR domain protein, endophilin-A2, in the clathrin-independent endocytosis of Shiga toxin in mammalian cells (Renard et al. 2015. Nature, 517(7535):493-496). I will also say a few words about what I am doing now at the UCLouvain, and more particularly about the hypothesis according to which BAR domain proteins constitute a protein family that define cargo specificity and is able to deform plasma membrane in clathrin-independent endocytic processes.

Published
2017

4. Role of curvature sensing/inducing BAR domain proteins in clathrin-independent endocytosis

Author

UCL - SST/ISV - Institut des sciences de la vie, Renard, Henri-François, Seminar of the "Institut de Biologie et de Médecine Moléculaires" (IBMM) from the "Université Libre de Bruxelles" (ULB), UCL - SST/ISV - Institut des sciences de la vie, Renard, Henri-François, and Seminar of the "Institut de Biologie et de Médecine Moléculaires" (IBMM) from the "Université Libre de Bruxelles" (ULB)

Abstract

Endocytosis is an essential cellular process required for uptake of nutrients from cell environment and turnover of plasma membrane components. Clathrin-mediated endocytosis is by far the best characterized endocytic process. Since the mid-90's, the existence of endocytic routes independent of clathrin emerged in mammalian cells. Therefore, the most challenging question in membrane biology rose: how can the plasma membrane be deformed in the absence of an organized clathrin coat? Until today, this question has not been fully solved. First attempts to shed light into this topic demonstrated the requirement of glycosphingolipids for membrane deformation in lectin-driven endocytosis (for instance, the endocytosis of the bacterial lectin Shiga toxin). Recently, BAR domain proteins (where BAR stands for Bin/Amphiphysin/Rvs) have been described as crucial for clathrin-independent endocytic routes. BAR domain proteins interact with membranes and act as curvature sensors/inducers. The function of many BAR domain proteins remains unclear in the landscape of clathrin-independent endocytosis, and especially regarding their role in the endocytic pit formation. The main part of my talk will be focused on the implication of a specific BAR domain protein, endophilin-A2, in the clathrin-independent endocytosis of Shiga toxin in mammalian cells (Renard et al. 2015. Nature, 517(7535):493-496). I will also say a few words about what I am doing now at the UCLouvain, and more particularly about the hypothesis according to which BAR domain proteins constitute a protein family that define cargo specificity and is able to deform plasma membrane in clathrin-independent endocytic processes.

Published
2017

5. Projet de recherche: Rhétorique & Robotique (Poster)

Author

Journée des Doctorants (23 mai 2017: Université Libre de Bruxelles (ULB)), Pieters, Céline, Journée des Doctorants (23 mai 2017: Université Libre de Bruxelles (ULB)), and Pieters, Céline

Abstract

info:eu-repo/semantics/nonPublished

Published
2017

6. Grève de la faim des sans-papiers :technique de corps pour forcer l'égalité ?

Author

Séminaire inter-universitaire : « Le corps en question. Réflexion épistémologique, conceptuelle et méthodologique sur la corporéité dans les sciences sociales (9 mai 2014: Ecole Doctorale Thématique en Sciences Sociales (www.edtss.be), Université libre de Bruxelles (ULB)), Vertongen, Youri Lou, Séminaire inter-universitaire : « Le corps en question. Réflexion épistémologique, conceptuelle et méthodologique sur la corporéité dans les sciences sociales (9 mai 2014: Ecole Doctorale Thématique en Sciences Sociales (www.edtss.be), Université libre de Bruxelles (ULB)), and Vertongen, Youri Lou

Abstract

info:eu-repo/semantics/nonPublished

Published
2014

7. La médiation au cœur des dispositifs de l’Etat social. Le cas des conseillers-emplois en Région de Bruxelles-Capitale.

Author

Secteur non marchand, milieux associatifs, organismes communautaires :des mondes en recomposition (2010-10-27/28: Université libre de Bruxelles (ULB)), Jacques, Wels, Secteur non marchand, milieux associatifs, organismes communautaires :des mondes en recomposition (2010-10-27/28: Université libre de Bruxelles (ULB)), and Jacques, Wels

Abstract

info:eu-repo/semantics/nonPublished

Published
2014

8. Extracting ANCs from elastic-scattering data

Author

ATHENA Brussels workshop on astrophysics (Jan. 27-28, 2014: Université libre de Bruxelles (ULB), Brussels, Belgium), Ramirez Suarez, Oscar Leonardo, Sparenberg, Jean-Marc, ATHENA Brussels workshop on astrophysics (Jan. 27-28, 2014: Université libre de Bruxelles (ULB), Brussels, Belgium), Ramirez Suarez, Oscar Leonardo, and Sparenberg, Jean-Marc

Abstract

Oral presentation by Oscar Ramírez, info:eu-repo/semantics/nonPublished

Published
2014

10. Antioxidant and anticancer activities of polyphenolic compounds from three Acanthaceae medicinal species from Burkina Faso

Author

Nabèrè, Ouattara; University of Ouagadougou, Samson, Guenné; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Adama, Hilou; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Moussa, Compaoré; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Eric, Sombié P.A.D.; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Aminata, Nacoulma P.; Laboratory of Toxicology, Institute of Pharmacy, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, B-1050 Brussels, Belgium., Jeanne, Millogo-Rasolodimby; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biology and plants Ecology of University of Ouagadougou, Germaine, Nacoulma Odile; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry (LABIOCA) Director, Nabèrè, Ouattara; University of Ouagadougou, Samson, Guenné; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Adama, Hilou; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Moussa, Compaoré; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Eric, Sombié P.A.D.; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry, Aminata, Nacoulma P.; Laboratory of Toxicology, Institute of Pharmacy, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, B-1050 Brussels, Belgium., Jeanne, Millogo-Rasolodimby; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biology and plants Ecology of University of Ouagadougou, and Germaine, Nacoulma Odile; University of Ouagadougou (Burkina Faso) UFR/SVT, 09 BP 848 Ouagadougou 09 Burkina Faso. Laboratory of Biochemistry and Applied Chemistry (LABIOCA) Director

Abstract

Phytochemical screening and biological activities of methanol extracts from three Acanthaceae species namely Hygrophila auriculata (H. auriculata), Nelsonia canescens (N. canescens) and Peristrophe bicalyculata (P. bicalyculata), widely used in local ethnomedicine, were carried out. Phenolic content quantification indicated that H. auriculata extract possessed the highest polyphenolics content followed by N. canescens and P. bicalyculata. Biological activities assessment showed that H. auriculata extract has the best antioxidant (through DPPH, FRAP and ABTS methods) capacity. Acetylcholinesterase and Lipoxygenase inhibition tests revealed interesting activities from P. bicalyculata. Testing cancer cells antiproliferation, it was showed that H. auriculata and N. canescens) were of good interest (27.00±1.55 and 29.70±3.11 µg/mL).These results showed that the phenolic compounds of these plants could justify their local traditional use to treat inflammatory and tumoral diseases.

Published
2013

11. La rétroaction négative en classe de grammaire. Enjeux démocratiques.

Author

"Si j'aurais sur, j'aurais pas venu." - Linguistique des formes exclues. (19-22 juin 2013: Université libre de Bruxelles (ULB)), Meinertzhagen, Lionel, "Si j'aurais sur, j'aurais pas venu." - Linguistique des formes exclues. (19-22 juin 2013: Université libre de Bruxelles (ULB)), and Meinertzhagen, Lionel

Abstract

Nous souhaitons montrer, sans généralisation, que le cours de français de primaire peine parfois - même souvent - à rencontrer la mission de l’école. En effet, on est en droit de craindre que les activités que l’on y rencontre et les rétroactions négatives qu’elles impliquent – elles dont l’objectif primaire est la correction et la remise face aux objectifs – ne participent pas à l’autonomisation des élèves et à la construction de leur esprit critique., info:eu-repo/semantics/nonPublished

Published
2013

13. Literary Journalism and History Panel

Author

6th Annual Conference of the International Association of Literary Journalism Studies (IALJS): Theoria, Poiesis and Praxis (12-14 mai 2011: Université libre de Bruxelles (ULB)), Meuret, Isabelle, Sims, Norman, 6th Annual Conference of the International Association of Literary Journalism Studies (IALJS): Theoria, Poiesis and Praxis (12-14 mai 2011: Université libre de Bruxelles (ULB)), Meuret, Isabelle, and Sims, Norman

Abstract

info:eu-repo/semantics/nonPublished

Published
2011

14. Le Journalisme littéraire à l’aube du XXe siècle: Regards croisés entre mondes anglophone et francophone

Author

Journalisme et littérature :problématiques de la longue durée et recherches en cours (26-27 mai 2011: Université libre de Bruxelles (ULB)), Meuret, Isabelle, Journalisme et littérature :problématiques de la longue durée et recherches en cours (26-27 mai 2011: Université libre de Bruxelles (ULB)), and Meuret, Isabelle

Abstract

info:eu-repo/semantics/nonPublished

Published
2011

15. Literary Journalism in Europe: Alternative Voices Narrating History

Author

Kobe University Brussels Days, Roles of Culture on the Foundation of European Integration (5 mars 2011: Université libre de Bruxelles (ULB)), Meuret, Isabelle, Kobe University Brussels Days, Roles of Culture on the Foundation of European Integration (5 mars 2011: Université libre de Bruxelles (ULB)), and Meuret, Isabelle

Abstract

info:eu-repo/semantics/nonPublished

Published
2011

16. Photooxidation of guanine by a ruthenium dipyridophenazine complex intercalated in a double-stranded polynucleotide monitored directly by picosecond visible and infrared transient absorption spectroscopy

Author

Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, University of Dublin, Trinity College (Ireland) - Chemistry and Physics, University of Nottingham (U.K.) - Chemistry, Rutherford Appleton Laboratory (Didcot - U.K.) - Central Laser Facility, Elias, Benjamin, Creely, Caitriona, Doorley, Gerard W., Feeney, Martin M., Moucheron, Cecile, Kirsch-DeMesmaeker, Andree, Dyer, Joanne, Grills, David C., George, Michael W., Matousek, Pavel, Parker, Anthony W., Towrie, Michael, Kelly, John M., Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, University of Dublin, Trinity College (Ireland) - Chemistry and Physics, University of Nottingham (U.K.) - Chemistry, Rutherford Appleton Laboratory (Didcot - U.K.) - Central Laser Facility, Elias, Benjamin, Creely, Caitriona, Doorley, Gerard W., Feeney, Martin M., Moucheron, Cecile, Kirsch-DeMesmaeker, Andree, Dyer, Joanne, Grills, David C., George, Michael W., Matousek, Pavel, Parker, Anthony W., Towrie, Michael, and Kelly, John M.

Abstract

Transient species formed by photoexcitation (400 nm) of [Ru(dppz)(tap)(2)](2+) (1) (dppz = dipyrido[3,2-a:2',3'-c]phenazine; tap = 1,4,5,8-tetraazaphenanthrene) in aqueous solution and when intercalated into a double-stranded synthetic polynucleotide, [poly(dG-dC)](2), have been observed on a picosecond timescale by both visible transient absorption (allowing monitoring of the metal complex intermediates) and transient infrared (IR) absorption spectroscopy (allowing direct study of the DNA nucleobases). By contrast with its behavior when free in aqueous solution, excitation of 1 when bound to [poly(dGdC), causes a strong increase in absorbance at 515 nm due to formation of the reduced complex [Ru(dppz)(tap)(2)](+) (rate constant = (2.0 +/- 0.2) x 10(9) s(-1)). The subsequent reformation of 1 proceeds with a rate constant of (1.1 +/- 0.2) x 10(8) s(-1). When the process is carried out in D2O, the rates of forma-tion and removal of [Ru(dppz)(tap)(2)](+) are reduced (rate constants (1.5 +/- 0.3) x 10(9) and (0.7 +/- 0.2) x 10(8) s(-1) respectively) consistent with proton-coupled electron transfer processes. Picosecond transient IR measurements in the 1540-1720 cm(-1) region in D2O solution confirm that the reduction of 1 intercalated into [poly(dG-dC)](2) is accompanied by bleaching of IR ground-state bands of guanine (1690 cm(-1)) and cytosine (1656 cm(-1)), each with similar rate constants.

Published
2008

17. Do women gain or lose from becoming mothers? A comparative wage analysis in 25 European countries

Author

“Parentalité et emploi – Un défi pour les politiques publiques”, conference organised by the Groupement de recherche européen du CNRS – Marché du travail et genre en Europe (MAGE) in collaboration with the Département d’économie appliquée of the Université Libre de Bruxelles (DULBEA) and the Unit Politiques et Services à l’Enfance of the Université Libre de Bruxelles (ULB-ESP) (18 December, 2008: Brussels, Belgium), O'Dorchai, Sile Padraigin, “Parentalité et emploi – Un défi pour les politiques publiques”, conference organised by the Groupement de recherche européen du CNRS – Marché du travail et genre en Europe (MAGE) in collaboration with the Département d’économie appliquée of the Université Libre de Bruxelles (DULBEA) and the Unit Politiques et Services à l’Enfance of the Université Libre de Bruxelles (ULB-ESP) (18 December, 2008: Brussels, Belgium), and O'Dorchai, Sile Padraigin

Abstract

info:eu-repo/semantics/nonPublished

Published
2008

18. Amino acids and proteins in photochemistry

Author

Université Paul Sabatier (Toulouse - France) - Laboratoire des IMRCP, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, UCL - Autre, Vicendo, Patricia, Bijeire, Laurent, Elias, Benjamin, Jouvet, Christophe, Kirsch-De Mesmaeker, Andree, Moucheron, Cecile, Université Paul Sabatier (Toulouse - France) - Laboratoire des IMRCP, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, UCL - Autre, Vicendo, Patricia, Bijeire, Laurent, Elias, Benjamin, Jouvet, Christophe, Kirsch-De Mesmaeker, Andree, and Moucheron, Cecile

Abstract

Photooxidation of proteins may result from a direct reactivity of the amino acids such as tyrosin or tryptophane with UV radiation. Recent data highlight the major role of the dissociative states of protonated aromatic amino acids in photofragmentation. This process can result from the loss of a hydrogen atom with formation of a radical cation. This will be a powerful tool to cleave selectively peptides. Proteins as DNA may also be the target of photosensitizers such as polyazaaromatic Ru-11 complexes. An important property of these compounds with very oxidative ligands is their very high oxidation power in the (MLCT)-M-3 (metal-to-ligand charge transfer) state. At the excited state, they are able to abstract an electron from electron donors such as the tryptophane. This reactivity can give rise to the formation of covalent photoadducts with the tryptophane. The formation of such damage displays a potent interest for the photodynamic therapy. Moreover, complex electron transfer reactions may also occur between ruthenium compounds under illumination and a metallo-protein such the superoxyde dismutase Cu/Zn leading to an inhibition of the enzymatic activity.

Published
2007

19. Individualiser la gestion des fins de carrière ? Du « vieillissement actif » à la généralisation de l’outplacement

Author

UCL - SSH/IACS - Institute of Analysis of Change in Contemporary and Historical Societies, Moulaert, Thibauld, Colloque organisé par le Centre de Recherche en Science Politique (CRESPO) des Facultés Universitaires Saint-Louis (FUSL), le Groupe de Recherche sur l'Action Publique (GRAP) de l'Université Libre de Bruxelles (ULB) et le Groupe de Sociologie Politique et Morale (GSPM) de l'École des Hautes Études en Sciences Sociales (EHESS) « Les approches pragmatiques de l’action publique », UCL - SSH/IACS - Institute of Analysis of Change in Contemporary and Historical Societies, Moulaert, Thibauld, and Colloque organisé par le Centre de Recherche en Science Politique (CRESPO) des Facultés Universitaires Saint-Louis (FUSL), le Groupe de Recherche sur l'Action Publique (GRAP) de l'Université Libre de Bruxelles (ULB) et le Groupe de Sociologie Politique et Morale (GSPM) de l'École des Hautes Études en Sciences Sociales (EHESS) « Les approches pragmatiques de l’action publique »

Published
2007

20. Dinuclear Ru(II)PHEHAT and -TPAC complexes: Effects of the second Ru-II center on their spectroelectrochemical properties

Author

Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Elias, Benjamin, Herman, Leslie, Moucheron, Cecile, Mesmaeker, Andree Kirsch-De, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Elias, Benjamin, Herman, Leslie, Moucheron, Cecile, and Mesmaeker, Andree Kirsch-De

Abstract

Four novel dinuclear Ru-II compounds and, for comparison purposes, two corresponding mononuclear complexes containing the PHEHAT or TPAC ligand (PHEHAT = 1,10-phenanthrolino[5,6-b]-1,4,5,8,9,12-hexaazatriphenylene and TPAC = tetrapyrido[3,2-a:2',3'-c:3" ,2"-h:2''',3'''-j]acridine) have been synthesized and characterized. Conclusions on the effects of dinucleation of these two bridging ligands can be drawn only for the compounds for which the results demonstrate that the bridging ligand is involved in the first electrochemical reduction and lowest emission energy. The behavior of these complexes, which is not always predictable, is discussed, and the differences are highlighted in this work. Interestingly, all of the compounds are luminescent except one dinuclear species, [(phen)(2)Ru-mu-PHEHAT-Ru(TAP)(2)](4+), which does not luminesce in MeCN and BuCN at room temperature.

Published
2007

21. Effects of protonation on the spectroscopic properties of tetrapyridoacridine (TPAC) mono- and dinuclear Ru(II) complexes in their ground and (MLCT)-M-3 excited states

Author

Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Herman, Leslie, Elias, Benjamin, Pierard, Frederic, Moucheron, Cecile, Mesmaeker, Andree Kirsch-De, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Herman, Leslie, Elias, Benjamin, Pierard, Frederic, Moucheron, Cecile, and Mesmaeker, Andree Kirsch-De

Abstract

The spectroscopic behavior of mono- and dinuclear Ru(II)) complexes (P, T, PP and TT, Figure 1) that contain the extended planar ligand tetrapyrido[3,2-a:2',3'-c:3 '',2 ''-h:2"',3"'-j]lacridine (TPAC) and either 1,10-phenanthroline (phen) or 1,4,5,8-tetraazaphenanthrene (tap) as ancillary ligands is examined in water and as a function of the pH. These four complexes luminesce in aqueous solution. The analyses of the data in absorption lead to the pK(a), values in the ground state, and the data in emission show that the excited (MLCT)-M-3 states are much more basic than the ground state. When the complex contains tap ligands (T and TT), a decrease in pH transforms the luminescent excited basic form into another luminescent excited protonated species, which emits more bathochromically. In contrast, with phen ancillary ligands (P and PP), the protonated excited state does not luminesce. The rate constant of first protonation of the (MLCT)-M-3 state is diffusion controlled, except for the dinuclear PP complex, whose protonation takes place on the nitrogen of the acridine motif. For P, in which the protonation process is the fastest, it would take place on the nitrogen atoms of the nonchelated phen moiety of the TPAC ligand. These results allow also us to gain information on the localization of the excited electron in the (MLCT)-M-3 state populated upon absorption as well as in the relaxed 3MLCT emissive state. Moreover as these complexes are interesting for their study with DNA, it can be concluded from these data that a portion of the excited species in interaction with DNA will be protonated.

Published
2007

22. Audiences and users: Conceptual shift, points of convergence and mutual enrichments

Author

USL-B - Pôle de Recherches sur la Communication et les Médias (PReCoM), Université libre de Bruxelles (ULB) - Département SIC, Patriarche, Geoffroy, IAMCR conference ‘Media, Communication, Information: Celebrating 50 Years of Theories and Practices’, USL-B - Pôle de Recherches sur la Communication et les Médias (PReCoM), Université libre de Bruxelles (ULB) - Département SIC, Patriarche, Geoffroy, and IAMCR conference ‘Media, Communication, Information: Celebrating 50 Years of Theories and Practices’

Abstract

Several studies have already focused on the concepts of audience (e.g. Carpentier, 2004) and public (e.g. Dayan, 2001), and the relation between them (e.g. Livingstone, 2005). The present study extends these investigations to the concept of user and examines how it is or can be theoretically positioned towards the ‘audience’. The first part of this paper asks why ‘users’ tends to substitute for ‘audience’. It is argued that the conceptual shift is driven by theoretical positions as well as technological innovations. The concept of use is indeed widely constructed in diverse research traditions raising the issue of what people do with or trough media objects. Some of these traditions belong to mass communication research (e.g. uses and gratifications approach, ethnographic studies of domestic audiences) while others have but a loose connection with the field (e.g. French sociology of uses). In any case, the concept of user brings into the focus issues which are not directly addressed by the concept of audience. Technological innovations are also responsible for the redefinition of the audience as users. In fact several dimensions of new media objects put into question the fundamental presumptions of what is an audience. This paper focuses on computerisation (i.e. one have to deal with the considerable technological part of new media), selection (i.e. selective access to information), interaction (i.e. computer-mediated communication between individuals) and co-production (i.e. participation of ordinary individuals in content production and description), and examines for each of these four dimensions the arguments which may speak in favour of ‘users’ and against ‘audience’. The second part of this study demonstrates that despite their specificities, the two concepts are being crossed by similar epistemological, theoretical and ideological debates, especially on the active vs. passive nature of audiences or users, and the micro vs. macro level of analysis. Moreover, it

Published
2007

23. Réalités et illusions: réformer sans tabou

Author

UCL - EUEN/CORE - Center for operations research and econometrics, Université Libre de Bruxelles (ULB) - ECARES: the European Centre for Advanced Research in Economics and Statistics, Hindriks, Jean, Castanheira, Micael, UCL - EUEN/CORE - Center for operations research and econometrics, Université Libre de Bruxelles (ULB) - ECARES: the European Centre for Advanced Research in Economics and Statistics, Hindriks, Jean, and Castanheira, Micael

Published
2006

24. Photo-reduction pf polyazaaromatic Ru(II) complexes by biomolecules and possible applications

Author

Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Elias, Benjamin, Kirsch-De Mesmaeker, Andrée, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Elias, Benjamin, and Kirsch-De Mesmaeker, Andrée

Abstract

An important property of the Ru(II) complexes that contain polyazaaromatic ligands such as TAP (1,4,5,8-tetraazaphenanthrene) and HAT (1,4,5,8,9,12-hexaazatriphenylene) is the very high oxidation potential of the 3MLCT (Metal to Ligand Charge Transfer) state. Therefore, under illumination, they are able to abstract an electron even from rather poor electron donors. In this work, we show how it is possible to take advantage of this property for applications of photo-induced electron transfer between these excited complexes and biopolymers such as DNA, oligonucletotides and oligopeptites. One of the consequences of the charge transfer processes is photocleavage of plasmid DNA, demonstrated some years ago by agarose gel electrophoresis and more recently detected and quantified by AFM. Another effect of the photo-induced electron transfer is the formation of photo-adduct(s) of the metal complex with the guanine units of oligonucleotides or with the tryptophan moieties of oligopeptides. In the photo-adducts with the guanine nucleobases, a covalent bond is formed between one of the polyazaaromatic ligands and the guanine base without destruction of the ligands sphere around the metal ion. Interestingly, the formation of these photo-adducts can be exploited in order to photo-crosslink two single-stranded oligonucleotides. Such photo-crosslinking via the metal complexes could lead to applications in biological diagnostic tests and possibly in vivo in the area of gene phototherapy.

Published
2006

25. Photoelectron transfer processes with ruthenium(II) polypyridyl complexes and Cu/Zn superoxide dismutase

Author

Université Paul Sabatier (Toulouse - France) - Laboratoire des IMRCP, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Bijeire, Laurent, Elias, Benjamin, Souchard, Jean-Pierre, Gicquel, Etienne, Moucheron, Cécile, Kirsch- De Mesmaeker, Andrée, Vicendo, Patricia, Université Paul Sabatier (Toulouse - France) - Laboratoire des IMRCP, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Bijeire, Laurent, Elias, Benjamin, Souchard, Jean-Pierre, Gicquel, Etienne, Moucheron, Cécile, Kirsch- De Mesmaeker, Andrée, and Vicendo, Patricia

Abstract

The processes that are photoinduced by [Ru(bpz)(3)](2+)(bpz) 2,2 '- bipyrazyl) in the presence of Cu/Zn superoxide dismutase (Cu/Zn SOD) are investigated by laser flash photolysis and electron paramagnetic resonance (EPR) spectroscopy; they are compared to those of the system [Ru(bpy)(3)(2+)-Cu/Zn SOD]. Although the mechanism is complicated, primary and secondary reactions can be evidenced. First, the excited [Ru(bpz)(3)](2+) complex is quenched reductively by Cu/Zn SOD with the production of a reduced complex and an oxidized enzyme. The oxidation site of Cu/Zn SOD is proposed to correspond to amino acids located on the surface of the protein. Afterward and only when this reductive electron transfer to the excited complex has produced enough oxidized protein, another electron-transfer process can be evidenced. In this case, however, the charge-transfer process takes place in the other direction, i.e., from the excited complex to the Cu-II center of the SOD with the formation of Ru-III and Cu-I species. This proposed mechanism is supported by the fact that [Ru(bpy)(3)](2+), which is less photo-oxidizing than [Ru(bpz)(3)](2+), exhibits no photoreaction with Cu/Zn SOD. Because Ru-III species are generated as intermediates with [Ru( bpz)(3)](2+), they are proposed to be responsible for the enhancement of [poly(dGdC)](2) and [poly(dA-dT)](2) oxidation observed when Cu/Zn SOD is added to the [ Ru( bpz)(3)](2+)- DNA system.

Published
2006

26. Le siècle

Author

Sécularisations (24/11/2006: Université Libre de Bruxelles (ULB), Belgique), Nobilio, Fabien, Sécularisations (24/11/2006: Université Libre de Bruxelles (ULB), Belgique), and Nobilio, Fabien

Abstract

Journée d'étude du Groupe de contact FNRS "Penser le religieux en Europe"., info:eu-repo/semantics/nonPublished

Published
2006

27. Dendritic tetranuclear Ru(II) complexes based on the nonsymmetrical PHEHAT bridging ligand and their building blocks: Synthesis, characterization, and electrochemical and photophysical properties

Author

Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Leveque, Julien, Elias, Benjamin, Moucheron, Cécile, Kirsch-De Mesmaeker, Andrée, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Leveque, Julien, Elias, Benjamin, Moucheron, Cécile, and Kirsch-De Mesmaeker, Andrée

Abstract

Dinuclear and tetranuclear Ru(II) compounds 1, 2, 3, and 4 based on the PHEHAT ligand (PHEHAT = 1,10-phenanthrolino[5,6-b]-1,4,5,8,9,12-hexaazatriphenylene) are prepared and characterized on the basis of the data for other related mononuclear species. Their electrochemical and spectroscopic behaviors are discussed. The nonspectroelectrochemical correlation obtained for 1, 2, 3, and 4 is explained on the basis of these data. From the behavior in emission, it is concluded that the internal energy transfer takes place from the core to the peripheral metallic units in 3 and 4.

Published
2005

28. The concept of audience in the age of the Internet. A meta-analysis

Author

USL-B - Autre, Université libre de Bruxelles (ULB) - Département SIC, Patriarche, Geoffroy, First European Communication Conference (ECREA). ‘50 years of communication research: Past and future’, USL-B - Autre, Université libre de Bruxelles (ULB) - Département SIC, Patriarche, Geoffroy, and First European Communication Conference (ECREA). ‘50 years of communication research: Past and future’

Abstract

This study consists in a meta-analysis of the ‘audience’ in communication research about the internet. The typology of audiences on which the meta-analysis is based combines two dimensions: the audience as collective actor and the audience as attending actor. The corpus is composed of 186 research papers about the internet published between 1994 and 2002 in six European and American communication journals. Results demonstrate that while the audience as collective actor is studied in a rather innovative way, the audience as attending actor is examined along traditional lines. Moreover, it appears that the ‘internet audience’ is not an international concept as European and American scholars study different kinds of audiences and internet objects. These findings point to several avenues of research for internet scholars in general and European ones in particular.

Published
2005

29. [Ru(TAP)2dppz]2+ : a DNA intercalating complex, which luminescences strongly in water and undergoes photo-induced proton-coupled electron transfer with guanosine-5'-monophosphate

Author

Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, University of Dublin, Trinity College (Ireland) - Chemistry, UCL - Autre, Ortmans, Isabelle, Elias, Benjamin, Kelly, John M., Moucheron, Cécile, Kirsch-De Mesmaeker, Andrée, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, University of Dublin, Trinity College (Ireland) - Chemistry, UCL - Autre, Ortmans, Isabelle, Elias, Benjamin, Kelly, John M., Moucheron, Cécile, and Kirsch-De Mesmaeker, Andrée

Abstract

The lowest excited state of [Ru(TAP)2(dppz)]2+ (TAP = 1,4,5,8-tetraazaphenanthrene, dppz = dipyridol[3,2-a:2',3'-c]phenazine) is strongly luminescent, even in water, and very oxidizing. Therefore it is able to oxidise not only guanosine-5'-monophosphate (GMP), as demonstrated by laser flash photolysis, but also guanine-containing polynucleotides such as calf thymus DNA and [poly(dG-dC)]2. The luminescence quenching was found to be faster in H2O than in D2O as is the back reaction, indicating that both process probably proceed by proton-coupled electron transfer. These properties, that are controlled by the triplet MLCT state in which the charge has been transferred from the Ru to a TAP ligand, contrast with those of the well known [Ru(phen)2(dppz)]2+.

Published
2004

31. Ultrafast transient absorption studies of ruthenium and rhenium dipyridophenazine complexes bound to DNA and polynucleotides

Author

University of Dublin, Trinity College (Ireland) - Physics, University of Dublin, Trinity College (Ireland) - Chemistry, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Rutherford Appleton Laboratory (Didcot - U.K.) - Central Laser Facility, University of Nottingham (U.K.) - Chemistry, Queens University Belfast (Northern Ireland) - Chemistry, Creely, Caitriona M., Kelly, J. M., Feeney, M. M., Hudson, S., Penedo, J. C., Blau, W. J., Elias, Benjamin, Kirsch-De Mesmaeker, Andrée, Matousek, P., Towrie, M., Parker, A. W., Dyer, J. S., George, M. W., Coates, C. G., Mc Garvey, J. J., University of Dublin, Trinity College (Ireland) - Physics, University of Dublin, Trinity College (Ireland) - Chemistry, Université Libre de Bruxelles (ULB) - Chimie Organique et Photochimie, Rutherford Appleton Laboratory (Didcot - U.K.) - Central Laser Facility, University of Nottingham (U.K.) - Chemistry, Queens University Belfast (Northern Ireland) - Chemistry, Creely, Caitriona M., Kelly, J. M., Feeney, M. M., Hudson, S., Penedo, J. C., Blau, W. J., Elias, Benjamin, Kirsch-De Mesmaeker, Andrée, Matousek, P., Towrie, M., Parker, A. W., Dyer, J. S., George, M. W., Coates, C. G., and Mc Garvey, J. J.

Abstract

We report on ultrafast pump and probe studies of biological systems, in the form of polynucleotide and calf thymus DNA complexes. Molecules for study are bound to the polynucleotides and probed in the visible region to observe changes in the absorption over time. Various dipyridophenazine metal complexes are studied alone and complexed with DNA or synthetic polynucleotides to investigate changes occurring in their excited states upon interacting with nucleobases. Transient absorption measurements are performed pumping at 400nm and probing from 450-700nm with pulse duration of 400fs.

Published
2003

32. Une lecture piagétienne de la pratique des jeux vidéo

Author

USL-B - Autre, Université libre de Bruxelles (ULB) - Département des sciences de l'information et de la communication, Patriarche, Geoffroy, USL-B - Autre, Université libre de Bruxelles (ULB) - Département des sciences de l'information et de la communication, and Patriarche, Geoffroy

Published
2003

33. Picosecond studies of ruthenium and rhenium dipyridophenazine complexes in solution and when bound to polynucleotides

Author

University of Dublin, Trinity College (Ireland) - Chemistry, University of Dublin, Trinity College (Ireland) - Physics, Université Libre de Bruxelles (ULB) - Chimie Organique Physique, Rutherford Appleton Laboratory (Didcot - U.K.) - Central Laser Facility, Kelly, J. M., Creely, C. M., Feeney, M. M., Hudson, S., Blau, W. J., Elias, Benjamin, Kirsh-De Mesmaeker, Andrée, Matousek, P., Towrie, M., Parker, A. W., University of Dublin, Trinity College (Ireland) - Chemistry, University of Dublin, Trinity College (Ireland) - Physics, Université Libre de Bruxelles (ULB) - Chimie Organique Physique, Rutherford Appleton Laboratory (Didcot - U.K.) - Central Laser Facility, Kelly, J. M., Creely, C. M., Feeney, M. M., Hudson, S., Blau, W. J., Elias, Benjamin, Kirsh-De Mesmaeker, Andrée, Matousek, P., Towrie, M., and Parker, A. W.

Published
2002

34. Les prises de risque en matière de SIDA sur un site universitaire

Author

La journée de prévention du SIDA de l’Université Libre de Bruxelles (ULB) (18-11-1996: Université Libre de Bruxelles, Bruxelles), Libert, Yves, La journée de prévention du SIDA de l’Université Libre de Bruxelles (ULB) (18-11-1996: Université Libre de Bruxelles, Bruxelles), and Libert, Yves

Abstract

info:eu-repo/semantics/nonPublished

Published
1996

35. Determining PD-L1 Status in Patients With Triple-Negative Breast Cancer: Lessons Learned From IMpassion130

Author

Jary, Marine, Liu, Wen-Wei, Yan, Dongyao, Bai, Isaac, Muranyi, Andrea, Colle, Elise, Brocheriou, Isabelle, Turpin, Anthony, Radosevic-Robin, Nina, Bourgoin, Pierre, Penault-Llorca, Frédérique, Cohen, Romain, Vernerey, Dewi, André, Thierry, Borg, Christophe, Shanmugam, Kandavel, Svrcek, Magali, Liu, Wen‐wei, Cooperator Multidisciplinary Oncology Group (GERCOR), CHU Clermont-Ferrand, Ventana Medical Systems, Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service d'oncologie médicale (CHRU Lille), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), CHU Saint-Antoine [AP-HP], Service d'Oncologie Médicale [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC), Breast Cancer Translational Research Laboratory, Institut Jules Bordet [Bruxelles], Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB)-Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Translational Cancer Research Unit [Antwerp], Philipps Universität Marburg = Philipps University of Marburg, Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Division of Pathology and Laboratory Medicine, Università degli Studi di Milano = University of Milan (UNIMI)-European Institute of Oncology [Milan] (ESMO), University of the Sunshine Coast (USC), Erasmus University Medical Center [Rotterdam] (Erasmus MC), Department of Pathology, Aberdeen University Medical School, Hospital Universitario Ramón y Cajal [Madrid], Universidad de Alcalá - University of Alcalá (UAH), VU Medical Center, Cell Biology and Biotherapy Unit, INT-Fondazione Pascale, Department of Oncology and Metabolism [Sheffield, UK], The University of Sheffield [Sheffield, U.K.], Institute for Surgical Pathology, University hospital of Zurich [Zurich], Service de pathologie [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Pathologie [CHU Saint-Antoine], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Service d'Oncologie Médicale [CHU Saint -Antoine], Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Service d'Anatomie et cytologie pathologiques = Service de Pathologie [CHU Pitié-Salpêtrière] (ACP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service d'Oncologie Médicale [CHU Saint-Antoine], HAL-SU, Gestionnaire, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), and Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)

Subjects
Cancer Research, Biopsy, Best practice, BluePrint 80-gene signature, Predictive, DNA Mismatch Repair, pMMR, B7-H1 Antigen, Cohort Studies, Breast cancer, MESH: Tumor Microenvironment, Tumor Microenvironment, MESH: B7-H1 Antigen, Prospective Studies, MESH: Lymphocytes, Tumor-Infiltrating, MESH: Cohort Studies, Oligometastatic colorectal cancer, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, high/low risk, immune profile, General Medicine, Immunohistochemistry, External quality assessment, 3. Good health, MESH: DNA Mismatch Repair, Oncology, Cytological techniques, Colonic Neoplasms, Molecular Medicine, HER2-low, Colorectal Neoplasms, PD-L1, Histology, Serum proteins, T lymphocytes, [SDV.CAN]Life Sciences [q-bio]/Cancer, Prognostic, Non-small cell lung carcinoma, Lymphocytes, Tumor-Infiltrating, Molecular diagnostics, Genetics, Humans, early breast cancer, MESH: Colonic Neoplasms, MESH: Humans, Liquid biopsy, gene expression profiles, MammaPrint 70-gene signature, Plasma proteins, Biomarker, MESH: Prospective Studies, digestive system diseases, Next-generation sequencing, Trastuzumab-deruxtecan, MESH: Tissue Fixation, hallmarks of cancer, MESH: Colorectal Neoplasms, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; In the era of immune checkpoint inhibitors, understanding the metastatic microenvironment of proficient mismatch repair/microsatellite stable (pMMR/MSS) colorectal cancer (CRC) is of paramount importance to both prognostication and the development of more effective novel therapies. In this study, primary and paired metastasis tissue samples were collected from patients with resectable metastatic CRC treated with adjuvant FOLFOX or peri-operative chemotherapy in the MIROX phase III prospective study. In total, 74 cancer tissues were stained for CD3, CD8, Forkhead box protein 3 (FOXP3), programmed cell death protein-1 (PD-1, invasive front, stromal, intra-epithelial compartments), and programmed death-ligand 1 (PD-L1, tumor, immune cells). The immune profiling of primary CRC had a limited value to predict the immune context of paired metastases for all markers but CD3+. The expression of CD8 and PD-L1 was higher in metastases after neoadjuvant FOLFOX. In metastases, both CD3 T cells at the invasive front and PD-L1 expressions on immune cells were predictive of better disease-free survival. These results show that the effect of FOLFOX on modifying the immune microenvironment in resected CRC metastases and measurement of PD-L1 expression and tumor-infiltrating CD8 T cells in pMMR/MSS metastatic tissue samples could improve treatment strategies of metastatic CRC patients.

Published
2022

36. Multivariate Expectiles, Expectile Depth and Multiple-Output Expectile Regression

Author

Daouia, Abdelaati, Paindaveine, Davy, Daouia, Abdelaati, Régression extrême avec applications à l'économétrie, l'environnement et à la finance - - ExtremReg2019 - ANR-19-CE40-0013 - AAPG2019 - VALID, Groupe de recherche en économie mathématique et quantitative (GREMAQ), Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-École des hautes études en sciences sociales (EHESS)-Centre National de la Recherche Scientifique (CNRS), Département de Mathématique [Bruxelles] (ULB), Faculté des Sciences [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), ANR, and ANR-19-CE40-0013,ExtremReg,Régression extrême avec applications à l'économétrie, l'environnement et à la finance(2019)

Subjects
Centrality regions, Multivariate expectiles, [STAT.ME] Statistics [stat]/Methodology [stat.ME], [STAT.TH] Statistics [stat]/Statistics Theory [stat.TH], [STAT.TH]Statistics [stat]/Statistics Theory [stat.TH], Statistical depth, [STAT.ME]Statistics [stat]/Methodology [stat.ME], B- ECONOMIE ET FINANCE, Multiple-output regression
Abstract

Despite the importance of expectiles in fields such as econometrics, risk management, and extreme value theory, expectile regression—or, more generally, M-quantile regression—unfortunately remains limited to single-output problems. To improve on this, we define hyperplane-valued multivariate M-quantiles that show strong advantages over their point-valued competitors. Our M-quantiles are directional in nature and provide centrality regions when all directions are considered. These regions define new statistical depths, the halfspace M-depths, that include the celebrated Tukey depth as a particular case. We study thoroughly the proposed M-quantiles, halfspace M-depths, and corresponding regions. M-depths not only provide a general framework to consider Tukey depth, expectile depth, Lr-depths, etc., but are also of interest on their own. However, since our original motivation was to consider multiple-output expectile regression, we pay more attention to the expectile case and show that expectile depth and multivariate expectiles enjoy distinctive properties that will be of primary interest to practitioners: expectile depth is maximized at the mean vector, is smoother than the Tukey depth, and exhibits surprising monotonicity properties that are key for computational purposes. Finally, our multivariate expectiles allow defining multiple-output expectile regression methods, that, in riskoriented applications in particular, are preferable to their analogs based on standard quantiles.

Published
2023

37. Post-cardiac surgery fungal mediastinitis: clinical features, pathogens and outcome

Author

Geoffroy Hariri, Mathieu Genoud, Vincent Bruckert, Samuel Chosidow, Emmanuel Guérot, Antoine Kimmoun, Nicolas Nesseler, Emmanuel Besnier, Fabrice Daviaud, David Lagier, Julien Imbault, David Grimaldi, Adrien Bouglé, Nicolas Mongardon, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Groupe de Recherche Clinique en Anesthésie Réanimation médecine PEriopératoire (GRC 29 - ARPE), Sorbonne Université (SU), Hôpitaux Universitaires de Genève (HUG), Centre Hospitalier Universitaire de Nice (CHU Nice), CHU Henri Mondor [Créteil], Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), CHU Pontchaillou [Rennes], Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CHU Rouen, Normandie Université (NU), Endothélium, valvulopathies et insuffisance cardiaque (EnVI), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre cardiologique du Nord (CCN), CHU Marseille, CHU Pessac, Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases, Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), IMRB - GEIC2O/'Genetic and Environmental Interactions in COPD, Cystic fibrosis and Other (rare) respiratory diseases' [Créteil] (U955 Inserm - UPEC), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), and None

Subjects
Mediastinitis, Fungus, Nosocomial infection, Aspergillus, Trichosporon, [SDV]Life Sciences [q-bio], Cardiac surgery, Healthcare-associated infection, Critical Care and Intensive Care Medicine, Candida
Abstract

Objectives The occurrence of mediastinitis after cardiac surgery remains a rare and severe complication associated with poor outcomes. Whereas bacterial mediastinitis have been largely described, little is known about their fungal etiologies. We report incidence, characteristics and outcome of post-cardiac surgery fungal mediastinitis. Methods Multicenter retrospective study among 10 intensive care units (ICU) in France and Belgium of proven cases of fungal mediastinitis after cardiac surgery (2009–2019). Results Among 73,688 cardiac surgery procedures, 40 patients developed fungal mediastinitis. Five were supported with left ventricular assist device and five with veno-arterial extracorporeal membrane oxygenation before initial surgery. Twelve patients received prior heart transplantation. Interval between initial surgery and mediastinitis was 38 [17–61] days. Only half of the patients showed local signs of infection. Septic shock was uncommon at diagnosis (12.5%). Forty-three fungal strains were identified: Candida spp. (34 patients), Trichosporon spp. (5 patients) and Aspergillus spp. (4 patients). Hospital mortality was 58%. Survivors were younger (59 [43–65] vs. 65 [61–73] yo; p = 0.013), had lower body mass index (24 [20–26] vs. 30 [24–32] kg/m2; p = 0.028) and lower Simplified Acute Physiology Score II score at ICU admission (37 [28–40] vs. 54 [34–61]; p = 0.012). Conclusion Fungal mediastinitis is a very rare complication after cardiac surgery, associated with a high mortality rate. This entity should be suspected in patients with a smoldering infectious postoperative course, especially those supported with short- or long-term invasive cardiac support devices, or following heart transplantation.

Published
2023

38. Involvement of chemerin and CMKLR1 in the progesterone decrease by PCOS granulosa cells

Author

Ingrid Langer, F. Guerif, Pascal Froment, Alice Bongrani, Claire Petit, Anthony Estienne, Namya Mellouk, Joëlle Dupont, Christelle Ramé, Ingrid Plotton, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Institut de Recherche Interdisciplinaire en Biologie Humaine et moléculaire = Insitute of Interdisciplinary Research [Bruxelles, Belgique] (IRIBHM), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)

Subjects
Infertility, Embryology, medicine.medical_specialty, endocrine system diseases, [SDV]Life Sciences [q-bio], CMKLR1, Mice, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, In vivo, Internal medicine, medicine, Animals, Humans, Chemerin, Receptor, Progesterone, 030304 developmental biology, 0303 health sciences, Granulosa Cells, 030219 obstetrics & reproductive medicine, biology, business.industry, Obstetrics and Gynecology, Cell Biology, Progesterone secretion, medicine.disease, Follicular fluid, female genital diseases and pregnancy complications, In vitro, Follicular Fluid, Reproductive Medicine, biology.protein, Intercellular Signaling Peptides and Proteins, Female, Receptors, Chemokine, Chemokines, business, Polycystic Ovary Syndrome
Abstract

Polycystic ovarian syndrome (PCOS) is the main cause of infertility in women. It is frequently associated with reduced progesterone production by human luteinised granulosa cells (hlGCs). However, the molecular mechanisms involved in these steroidogenesis alterations in PCOS patients are unclear. In a dihydrotestosterone-induced PCOS mouse model, steroid production is maintained in the setting of chemokine-like receptor 1 (Cmklr1) knockout. Thus, chemerin and chemerin receptors in terms of expression and progesterone regulation could be different in control and PCOS hlGCs. We first confirmed that progesterone levels in both plasma (P < 0.0001) and follicular fluid (FF) (P < 0.0001) were significantly reduced in PCOS normal weight women compared to control women. These data were associated with a lower STAR mRNA expression in both in vivo (P < 0.0001) and in vitro (P < 0.0001) hlGCs from PCOS women. Secondly, chemerin FF levels (P < 0.0001) and RARRES2 (P < 0.05) and CMKLR1 (P < 0.0001) mRNA levels in GCs were higher in PCOS normal weight patients. Thirdly, treatment of hlGCs with a specific nanobody (the VHH CA4910) targeting the human receptor for CMKLR1 leading to its inactivation abolished chemerin-induced progesterone inhibition, suggesting the involvement of CMKLR1 in this process. Furthermore, the inhibition of progesterone secretion induced by chemerin was two-fold higher in PCOS hlGCs (P < 0.05). Moreover, the VHH CA4910 reinstated a normal progesterone secretion with lower concentrations in PCOS hlGCs, suggesting a different chemerin sensitivity between PCOS and control hlGCs. Thus, chemerin, through CMKLR1, could be involved in the steroidogenesis alterations in PCOS hlGCs.

Published
2021

39. Expert opinion on NSCLC small specimen biomarker testing — Part 2: Analysis, reporting, and quality assessment

Author

Frédérique Penault-Llorca, Keith M. Kerr, Pilar Garrido, Erik Thunnissen, Elisabeth Dequeker, Nicola Normanno, Simon J. Patton, Jenni Fairley, Joshua Kapp, Daniëlle de Ridder, Aleš Ryška, Holger Moch, Breast Cancer Translational Research Laboratory, Institut Jules Bordet [Bruxelles], Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB)-Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Translational Cancer Research Unit [Antwerp], Charité, Institute of Pathology, Translational Tumorpathology Unit, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Division of Pathology and Laboratory Medicine, Università degli Studi di Milano = University of Milan (UNIMI)-European Institute of Oncology [Milan] (ESMO), University of the Sunshine Coast (USC), Erasmus University Medical Center [Rotterdam] (Erasmus MC), University of Zurich, and Moch, Holger

Subjects
MESH: Expert Testimony, Lung Neoplasms, MESH: Mutation, CELL LUNG-CANCER, 610 Medicine & health, Best practice, [SDV.CAN]Life Sciences [q-bio]/Cancer, MOLECULAR-PATHOLOGY, RECOMMENDATIONS, Pathology and Forensic Medicine, Non-small cell lung carcinoma, 1307 Cell Biology, Breast cancer, Carcinoma, Non-Small-Cell Lung, 10049 Institute of Pathology and Molecular Pathology, RET FUSIONS, Pathology, 1312 Molecular Biology, Molecular diagnostics, Humans, Expert Testimony, Molecular Biology, MESH: High-Throughput Nucleotide Sequencing, OUTCOMES, Science & Technology, MESH: Humans, Liquid biopsy, High-Throughput Nucleotide Sequencing, Cell Biology, General Medicine, MUTATION STATUS, DNA, Biomarker, IN-SITU HYBRIDIZATION, ONCOLOGY, Immunohistochemistry, External quality assessment, MESH: Lung Neoplasms, 2734 Pathology and Forensic Medicine, MESH: Reproducibility of Results, Mutation, Trastuzumab-deruxtecan, Next-generation sequencing, MESH: Biomarkers, HER2-low, Life Sciences & Biomedicine, Biomarkers, MESH: Carcinoma, Non-Small-Cell Lung
Abstract

The diagnostic work-up for non-small cell lung cancer (NSCLC) requires biomarker testing to guide therapy choices. This article is the second of a two-part series. In Part 1, we summarised evidence-based recommendations for obtaining and processing small specimen samples (i.e. pre-analytical steps) from patients with advanced NSCLC. Here, in Part 2, we summarise evidence-based recommendations relating to analytical steps of biomarker testing (and associated reporting and quality assessment) of small specimen samples in NSCLC. As the number of biomarkers for actionable (genetic) targets and approved targeted therapies continues to increase, simultaneous testing of multiple actionable oncogenic drivers using next-generation sequencing (NGS) becomes imperative, as set forth in European Society for Medical Oncology guidelines. This is particularly relevant in advanced NSCLC, where tissue specimens are typically limited and NGS may help avoid tissue exhaustion compared with sequential biomarker testing. Despite guideline recommendations, significant discrepancies in access to NGS persist across Europe, primarily due to reimbursement constraints. The use of increasingly complex testing methods also has implications for the reporting of results. Molecular testing reports should include clinical interpretation with additional commentary on sample adequacy as appropriate. Molecular tumour boards are recommended to facilitate the interpretation of complex genetic information arising from NGS, and to collaboratively determine the optimal treatment for patients with NSCLC. Finally, whichever testing modality is employed, it is essential that adequate internal and external validation and quality control measures are implemented. ispartof: VIRCHOWS ARCHIV vol:481 issue:3 pages:351-366 ispartof: location:Germany status: published

Published
2022

40. Transaction fees optimization in the Ethereum blockchain

Author

Arnaud Laurent, Luce Brotcorne, Bernard Fortz, Integrated Optimization with Complex Structure (INOCS), Inria Lille - Nord Europe, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université libre de Bruxelles (ULB)-Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 (CRIStAL), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Département d'Informatique [Bruxelles] (ULB), Faculté des Sciences [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), and Institut National de Recherche en Informatique et en Automatique (Inria)

Subjects
Optimization, Ethereum, Blockchain, General Engineering, Gasprice, General Earth and Planetary Sciences, [INFO.INFO-RO]Computer Science [cs]/Operations Research [cs.RO], Monte-Carlo, Ether, ComputingMilieux_MISCELLANEOUS, Pricing, General Environmental Science
Abstract

International audience; In blockchains, transaction fees are fixed by the users. The probability for a transaction to be processed quickly increases with the fee level. In this paper, we study the transaction fee optimization problem in the Ethereum blockchain. This problem consists of determining the minimum price a user should pay so that its transaction is processed with a given probability in a given amount of time. To reach this goal, we define a new solution method based on a Monte Carlo approach to predict the probability that a transaction will be mined within a given time limit. Numerical results on real data highlight the quality of the results.

Published
2022

41. Review of Clinical and Technological Consideration for MRI-Guided Robotic Prostate Brachytherapy

Author

Nick Reynaert, Taha Chettibi, Wojciech Polak, Sarah Wilby, Antony L. Palmer, Sepaldeep Singh Dhaliwal, Rochdi Merzouki, Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 (CRIStAL), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université Saâd Dahlab Blida 1 (UB1), Portsmouth Hospitals NHS Trust, Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB), Institut Jules Bordet [Bruxelles], Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), This work was supported by the European regional development Fund under EU Interreg 2 Seas, CoBra - Cooperative Brachytherapy, European Project: 2S04-022,CoBra, and Université de Saâd Dahlab [Blida] (USDB )

Subjects
medicine.medical_specialty, Computer science, medicine.medical_treatment, Brachytherapy, Image registration, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, Prostate intervention, Robotized Prostate Brachytherapy, Intraoperative MRI, [SPI]Engineering Sciences [physics], Focal therapy, MRI Robot, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, medicine, Medical imaging, [INFO.INFO-RB]Computer Science [cs]/Robotics [cs.RO], [INFO]Computer Science [cs], Medical physics, Prostate cancer, medicine.diagnostic_test, Low-Dose Rate Brachytherapy, 3. Good health, Transrectal ultrasonography, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Prostate brachytherapy
Abstract

International audience; Low Dose Rate Brachytherapy (LDR-BT) is a technique for treating localized prostate cancer by implanting radioactive seeds. In conventional practice, the delivery of seeds is performed using transrectal ultrasonography (TRUS) imaging for implant guidance and checked using computed-tomography for post-implant dosimetry. In the case of TRUS, accuracy can be compromised due to sub-optimal imaging. Magnetic Resonance Imaging (MRI), however, is known to provide better soft-tissue contrast, therefore, increasing the ability to detect small lesions; for that reason, the integration of intraoperative MRI in BT workflows has been investigated over the last two decades. The fusion of preoperative MR-images during TRUS-brachytherapy is possible. However, the image registration process introduces a source of uncertainty. Manual, real-time intra-operative LDR-BT is challenging under MRI due to confined space and procedural workflows. This motivates the development of MRI-compatible robots for prostate BT, with potential advantages of improved source placement accuracy and final dosimetry. In this paper, the state-of-art of technological components in MRI compatible robots, especially for LDR-BT, has been presented. This systematic review helps us to position an ongoing Cooperative Brachytherapy project, developing a real-time MRI-guided robot for adaptive LDR-BT. The design approach includes integrating separate modules: imaging, dose planning, needles, and robot.

Published
2021

42. Development of fluorizoline analogues as prohibitin ligands that modulate C-RAF signaling, p21 expression and melanogenesis

Author

Nora Chouha, Hussein Abou-Hamdan, Hajime Yurugi, Riku Yoshii, Hiromi Ii, Ahmad Najem, Ghanem E. Ghanem, Susumu Nakata, Krishnaraj Rajalingam, Yu Peng, Dong Wang, Canan G. Nebigil, Laurent Désaubry, Nanomédecine Régénérative (NanoRegMed), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Biomédecine de Strasbourg (CRBS), Laboratoire de Chimie et Chimie de l'Environnement (LCCE), Université Hadj Lakhdar Batna 1, University Medical Center [Mainz], Kyoto University, Institut Jules Bordet [Bruxelles], Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Université libre de Bruxelles (ULB), Tianjin University of Science and Technology (TUST), Xinjiang University, and univOAK, Archive ouverte

Subjects
Cyclin-Dependent Kinase Inhibitor p21, Melanins, Pharmacology, Melanogenesis, p21, [CHIM.THER] Chemical Sciences/Medicinal Chemistry, Organic Chemistry, Apoptosis, General Medicine, Heterocycles, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Protein Serine-Threonine Kinases, Ligands, Proto-Oncogene Proteins c-raf, Repressor Proteins, MAP kinases, Proto-Oncogene Proteins, Drug Discovery, Prohibitins, Humans, Chimie/Chimie thérapeutique, HeLa Cells, Transcription Factors, Cancer
Abstract

Fluorizoline is a cytotoxic trifluorothiazoline that targets the scaffold proteins prohibitins-1 and -2 (PHB1/2) to inhibit the kinase C-RAF and promote the expression of the cyclin-dependent kinase inhibitor p21 to induce cancer cell death. In melanocytes, fluorizoline also induces the synthesis of melanin. Herein we report the first structural requirement of fluorizoline analogues for these activities. We identified in particular some compounds that display enhanced anti-C-RAF and anti-MEK activities, and a higher cytotoxicity in HeLa cells compared to fluorizoline. These results provide a foundation for further optimization of PHB ligands for the treatment of cancers. We also discovered an analogue of fluorizoline that displays pharmacological effects opposed to those of fluorizoline and that can be used as a chemical tool to explore PHB signaling in cancers and other diseases.

Published
2022

43. Exercise in lung Cancer, the healthcare providers opinion (E.C.H.O.): Results of the EORTC lung cancer Group (LCG) survey

Author

Sara Pilotto, Alice Avancini, Jessica Menis, Isabella Sperduti, Matteo Giaj Levra, Thierry Berghmans, Paolo Bironzo, Mariana Brandão, Dirk De Ruysscher, John Edwards, Corinne Faivre-Finn, Nicolas Girard, Laurent Greillier, Lizza Hendriks, Sylvie Lantuejoul, Murielle Mauer, Silvia Novello, Mary O'Brien, Martin Reck, Noemi Reguart, Jordi Remon, Jan von der Thüsen, Anne-Marie C. Dingemans, Benjamin Besse, Michele Milella, Centre Hospitalier Universitaire [Grenoble] (CHU), Institut Jules Bordet [Bruxelles], Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto = University of Porto, Maastricht University Medical Centre (MUMC), Maastricht University [Maastricht], Royal Holloway [University of London] (RHUL), University of Manchester [Manchester], Méthodes computationnelles pour la prise en charge thérapeutique en oncologie : Optimisation des stratégies par modélisation mécaniste et statistique (COMPO), Inria Sophia Antipolis - Méditerranée (CRISAM), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Multidisciplinary Oncology and Therapeutic Innovations Unit, Hôpital Nord [CHU - APHM], Département d'hématologie [Gustave Roussy], Institut Gustave Roussy (IGR), School for Oncology and Developmental Biology [Maastricht] (GROW), Maastricht University [Maastricht]-Maastricht University Medical Centre (MUMC), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), European Organisation for Research and Treatment of Cancer [Bruxelles] (EORTC), European Cancer Organisation [Bruxelles] (ECCO), Università degli studi di Torino = University of Turin (UNITO), German Center for Lung Research, August Pi i Sunyer Biomedical Research Institute - IDIBAPS [Barcelona, Spain], Centro Integral Oncologico Clara Campal, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, Radiotherapie, Pulmonologie, Pathology, and Pulmonary Medicine

Subjects
Pulmonary and Respiratory Medicine, REHABILITATION, Cancer Research, Lung Neoplasms, Facilitators, Clinicians, Health Personnel, [SDV.CAN]Life Sciences [q-bio]/Cancer, AMERICAN-COLLEGE, GUIDELINES, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, MESH: Referral and Consultation, SDG 3 - Good Health and Well-being, QUALITY-OF-LIFE, Surveys and Questionnaires, Humans, Barriers, Current practice, Exercise, Lung cancer, Oncologist, Physical activity, Recommendation, Referral and Consultation, MESH: Surveys and Questionnaires, MEDICINE ROUND-TABLE, MESH: Humans, RANDOMIZED CONTROLLED-TRIAL, CHEMOTHERAPY, MESH: Lung Neoplasms, PHYSICAL-ACTIVITY, Oncology, MESH: Exercise, MESH: Health Personnel, INTERVENTION
Abstract

Objectives: Exercise has been reported to alleviate disease as well as treatment impact in patients with lung cancer. Nevertheless, there is limited information available regarding the perception of lung cancer dedicated healthcare professionals’ and their advice on exercise. Materials and Methods: An online survey exploring healthcare professionals’ practice patterns, perceptions, barriers, and facilitators of exercise in patients with lung cancer was conducted within members of the EORTC Lung Cancer Group (LCG). Results: One hundred forty-one healthcare providers completed the survey, mainly medical and radiation oncologists. Overall, 63% of the study participants declared that they frequently assessed exercise level in their patients, and 43% of them reinforced the importance of exercise. However, only 10% referred patients to an exercise program or specialist. Although the majority of the respondents had a positive perception regarding the benefits and safety of exercise (even in patients with advanced disease and/or bone metastasis), two-thirds of clinicians reported not having adequate training about exercise counselling. Moreover, 53% reported to lack of knowledge of guidelines referring to exercise in patients with cancer. Several obstacles and facilitators to improve exercise promotion in lung cancer care were identified. Conclusion: Healthcare providers recognize the relevance and feasibility of exercise as part of cancer treatment intervention, but specific pathways to do the referral are frequently missing. Future structured and well-designed strategies and initiatives are needed to support an effective referral in order to implement exercise interventions routinely in clinical practice.

Published
2022

44. Influence of Speech and Cognitive Load on Balance and Timed up and Go

Author

Olivier Van Hove, Romain Pichon, Pauline Pallanca, Ana Maria Cebolla, Sarah Noel, Véronique Feipel, Gaël Deboeck, Bruno Bonnechère, Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Institut de formation en Pédicurie-Podologie, Ergothérapie, Masso-Kinésithérapie (IFPEK), Laboratoire Mouvement Sport Santé (M2S), Université de Rennes (UR)-École normale supérieure - Rennes (ENS Rennes)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université libre de Bruxelles (ULB), and Hasselt University (UHasselt)

Subjects
balance, dual task, cognitive loads, Wii balance board, timed up and go, General Neuroscience, [SDV]Life Sciences [q-bio]
Abstract

International audience; The interaction between oral and/or mental cognitive tasks and postural control and mobility remains unclear. The aim of this study was to analyse the influence of speech production and cognitive load levels on static balance and timed up and go (TUG) during dual-task activities. Thirty healthy young subjects (25 ± 4 years old, 17 women) participated in this study. A control situation and two different cognitive arithmetic tasks were tested: counting backward in increments of 3 and 7 under oral (O) and mental (M) conditions during static balance and the TUG. We evaluated the dual-task cost (DTC) and the effect of speech production (SP) and the level of cognitive load (CL) on these variables. There was a significant increase in the centre of pressure oscillation velocity in static balance when the dual task was performed orally compared to the control situation The DTC was more pronounced for the O than for the M. The SP, but not the CL, had a significant effect on oscillation velocity. There was an increase in TUG associated with the cognitive load, but the mental or oral aspect did not seem to have an influence. Mobility is more affected by SP when the cognitive task is complex. This may be particularly important for the choice of the test and understanding postural control disorders.

Published
2022

45. Integrated Genomic Analysis Identifies Driver Genes and Cisplatin-Resistant Progenitor Phenotype in Pediatric Liver Cancer

Author

Jean-François Deleuze, Léa Meunier, Amélie Roehrig, Emmanuel Jacquemin, Victor Renault, Florent Guérin, Stefano Caruso, Sophie Taque, Isabelle Aerts, Catherine Guettier, Monique Fabre, Emmanuel Gonzales, Jill Pilet, Brice Fresneau, Sandra Rebouissou, Laura Molina, C. Chardot, Eric Letouzé, Benedict J.E. Monteiro, Véronique Laithier, Eric Trepo, Quentin Bayard, Jessica Zucman-Rossi, Guillaume Morcrette, Sophie Branchereau, Laurence Brugières, Théo Z. Hirsch, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), University of Pittsburgh (PITT), Pennsylvania Commonwealth System of Higher Education (PCSHE), Génomique Fonctionnelle des Tumeurs Solides (U1162), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Fondation Jean Dausset CEPH, Centre National du Génotypage [Evry], Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Institut Curie [Paris], CHU Pontchaillou [Rennes], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Hôpital Paul Brousse, Institut Gustave Roussy (IGR), Département de cancérologie de l'enfant et de l'adolescent [Gustave Roussy], Zucman-Rossi, Jessica, and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité)

Subjects
Hepatoblastoma, Male, Carcinoma, Hepatocellular, Adolescent, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Antineoplastic Agents, Locus (genetics), Biology, Article, Pediatric liver cancer, Metastasis, 03 medical and health sciences, 0302 clinical medicine, Integrated genomic analysis, medicine, Humans, Progenitor cell, Child, Gene, 030304 developmental biology, Progenitor, 0303 health sciences, Chemotherapy, Cancer stem cells, Gene Expression Profiling, Liver Neoplasms, Infant, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Genomics, Sciences bio-médicales et agricoles, medicine.disease, Phenotype, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, 3. Good health, [SDV] Life Sciences [q-bio], Oncology, Drug Resistance, Neoplasm, Cell culture, Child, Preschool, 030220 oncology & carcinogenesis, Cancer research, Female, Cisplatin, Neoplasm Recurrence, Local, Chemotherapy resistance
Abstract

Pediatric liver cancers (PLCs) comprise diverse diseases affecting infants, children and adolescents. Despite overall good prognosis, PLCs display heterogeneous response to chemotherapy. Integrated genomic analysis of 126 pediatric liver tumors showed a continuum of driver mechanisms associated with patient age, including new targetable oncogenes. In 10% of hepatoblastoma patients, all before 3 years old, we identified a mosaic premalignant clonal expansion of cells altered at the 11p15.5 locus. Analysis of spatial and longitudinal heterogeneity revealed an important plasticity between 'Hepatocytic', 'Liver Progenitor' and 'Mesenchymal' molecular subgroups of hepatoblastoma. We showed that during chemotherapy, 'Liver Progenitor' cells accumulated massive loads of cisplatin-induced mutations with a specific mutational signature, leading to the development of heavily mutated relapses and metastases. Drug screening in PLC cell lines identified promising targets for cisplatin-resistant progenitor cells, validated in mouse xenograft experiments. These data provide new insights into cisplatin resistance mechanisms in PLC and suggest alternative therapies., info:eu-repo/semantics/published

Published
2021

46. Health-related quality of life and utility outcomes with selinexor in relapsed/refractory diffuse large B-cell lymphoma

Author

Agnes Nagy, Rene-Olivier Casasnovas, Sameer Bakhshi, Sharon Shacham, Juan-Manuel Sancho, Ronit Gurion, Josée M. Zijlstra, Jatin P. Shah, Miklos Egyed, Brian T. Hill, Krzysztof Warzocha, Gabriel Tremblay, Michael W. Schuster, Fritz Offner, Reda Bouabdallah, Dimitrios Tomaras, Paolo Caimi, Andre Goy, Priyanka Samal, Catherine Thieblemont, Joost S.P. Vermaat, Matthew Ku, Ulrich Jäger, John Kuruvilla, Nagesh Kalakonda, George A Follows, Eric Van Den Neste, Miguel Ángel Canales Albendea, Michael Kauffman, Nada Hamad, Fatima De la Cruz, Patrick Daniele, Marie Maerevoet, Theodoros Vasilakopoulos, Federica Cavallo, Sylvain Choquet, Karyopharm Therapeutics Inc., Newton, MA, U918, Service d'Hématologie Clinique (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Institut Jules Bordet [Bruxelles], Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Amsterdam UMC, Addenbrooke's Hospital, Cambridge University NHS Trust, Leiden University Medical Center (LUMC), Institute of Translational Medicine, University of Liverpool, Hackensack University Medical Center [Hackensack], Service d'Hématologie clinique [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Catholique de Louvain = Catholic University of Louvain (UCL), Cleveland Clinic, Service d'Hémato-oncologie [CHU Saint-Louis], Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), University of Turin, Hospital Universitario Virgen del Rocío [Sevilla], University Health Network, St Vincent's Hospital Melbourne [Australia], Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Medizinische Universität Wien = Medical University of Vienna, University Hospitals Case Medical Center, Tel Aviv University [Tel Aviv], Institute of Hematology and Transfusion Medicine[Warsaw, Poland] (IHTM), Germans Trias i Pujol Hospital, Barcelona Autonomous University, Stony Brook University [SUNY] (SBU), State University of New York (SUNY), Ghent University Hospital, National and Kapodistrian University of Athens (NKUA), Semmelweis University [Budapest], Hospital Universitario La Paz, UCL - SSS/DDUV - Institut de Duve, UCL - SSS/DDUV/BCHM - Biochimie-Recherche métabolique, and UCL - (SLuc) Service d'hématologie

Subjects
Male, Oncology, Cancer Research, Health utility, FACT-Lym, patient reported outcomes, MESH: Patient Reported Outcome Measures, MESH: Aged, 80 and over, 0302 clinical medicine, Quality of life, Recurrence, Medicine and Health Sciences, Aged, 80 and over, MESH: Aged, MESH: Middle Aged, health, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, General Medicine, Middle Aged, MESH: Drug Resistance, Neoplasm, 3. Good health, health-related quality of life, Hydrazines, EQ-5D-5L, patient-reported outcomes, 030220 oncology & carcinogenesis, Female, health utility, Lymphoma, Large B-Cell, Diffuse, MESH: Hydrazines, Adult, disutility of adverse events, medicine.medical_specialty, diffuse large B-cell lymphoma, [SDV.CAN]Life Sciences [q-bio]/Cancer, 03 medical and health sciences, Refractory, Internal medicine, medicine, Humans, health state utility, selinexor, Patient Reported Outcome Measures, Aged, Health related quality of life, MESH: Humans, business.industry, MESH: Quality of Life, MESH: Adult, Triazoles, medicine.disease, MESH: Male, MESH: Recurrence, Lymphoma, MESH: Triazoles, utility, Drug Resistance, Neoplasm, Relapsed refractory, Quality of Life, MESH: Lymphoma, Large B-Cell, Diffuse, business, MESH: Female, Diffuse large B-cell lymphoma, Progressive disease, 030215 immunology
Abstract

Aim: Evaluate health-related quality of life (HRQoL) and health utility impact of single-agent selinexor in heavily pretreated patients with relapsed/refractory diffuse large B-cell lymphoma. Patients & methods: Functional Assessment of Cancer Therapy (FACT) - Lymphoma and EuroQoL five-dimensions five-levels data collected in the single-arm Phase IIb trial SADAL (NCT02227251) were analyzed with mixed-effects models. Results: Treatment responders maintained higher FACT - Lymphoma (p = 3 adverse events and serious adverse events were not associated with clinically meaningful negative QoL impacts.

Published
2021

47. Approximate Normal Forms via Floquet–Bloch Theory: Nehorošev Stability for Linear Waves in Quasiperiodic Media

Author

Christopher Shirley, Antoine Gloria, Mitia Duerinckx, Laboratoire de Mathématiques d'Orsay (LMO), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université libre de Bruxelles (ULB), Laboratoire Jacques-Louis Lions (LJLL (UMR_7598)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Département de Mathématique [Bruxelles] (ULB), Faculté des Sciences [Bruxelles] (ULB), and Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB)

Subjects
Floquet theory, FOS: Physical sciences, Lambda, 01 natural sciences, Stability (probability), Mathematics - Spectral Theory, Mathematics - Analysis of PDEs, [MATH.MATH-MP]Mathematics [math]/Mathematical Physics [math-ph], 0103 physical sciences, FOS: Mathematics, [MATH.MATH-AP]Mathematics [math]/Analysis of PDEs [math.AP], Ergodic theory, [MATH]Mathematics [math], 0101 mathematics, Nonlinear Sciences::Pattern Formation and Solitons, Spectral Theory (math.SP), Mathematical Physics, Physique théorique et mathématique, Mathematical physics, Physics, Operator (physics), 010102 general mathematics, Statistical and Nonlinear Physics, Mathematical Physics (math-ph), 16. Peace & justice, Exponential function, Mathématiques, Flow (mathematics), Quasiperiodic function, 010307 mathematical physics, Analyse mathématique, Analysis of PDEs (math.AP), [MATH.MATH-SP]Mathematics [math]/Spectral Theory [math.SP]
Abstract

We study the long-time behavior of the Schrodinger flow in a heterogeneous potential $$\lambda V$$ with small intensity $$00$$ . More precisely, the approximate normal form leads to an accurate long-time description of the Schrodinger flow as an effective unitary correction of the free flow. The approach is robust and generically applies to linear waves. For classical waves, for instance, this allows to extend diffractive geometric optics to quasiperiodically perturbed media.

Published
2021

48. Deciding feasibility of a booking in the European gas market on a cycle is in P for the case of passive networks

Author

Johannes Thürauf, Fränk Plein, Martine Labbé, Martin Schmidt, Integrated Optimization with Complex Structure (INOCS), Inria Lille - Nord Europe, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université libre de Bruxelles (ULB)-Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 (CRIStAL), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Département d'Informatique [Bruxelles] (ULB), Faculté des Sciences [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Trier University, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), and Martine Labbé has been partially supported by the Fonds de la Recherche Scientifique - FNRS under Grant no PDR T0098.18. Fränk Plein thanks the Fonds de la Recherche Scientifique - FNRS for his Aspirant fellowship supporting the research for this publication. He also thanks the Deutsche Forschungsgemeinschaft for their support within the project Z01 in CRC TRR 154. This research has been performed as part of the Energie Campus Nürnberg and is supported by funding of the Bavarian State Government. The third and fourth author also thank the DFG for their support within projects A05, B07, and B08 in CRC TRR 154. Finally, we want to thank Lars Schewe for many fruitful discussions on the topic of this paper.

Subjects
[INFO.INFO-CC]Computer Science [cs]/Computational Complexity [cs.CC], Gas networks, Mathematical optimization, Computational complexity theory, Computer Networks and Communications, Computer science, Dimension (graph theory), 0211 other engineering and technologies, System of polynomial equations, Modèles mathématiques d'aide à la décision, 02 engineering and technology, Cycle, Optimisation de réseaux, Informatique mathématique, 0502 economics and business, Real algebraic geometry, Time complexity, European entry-exit market, 050210 logistics & transportation, computational complexity, 021103 operations research, cycle, 05 social sciences, [INFO.INFO-RO]Computer Science [cs]/Operations Research [cs.RO], Maximization, potential-based flows, Potential-based flows, Computational complexity, Nonlinear system, Bookings, Flow (mathematics), bookings, Hardware and Architecture, 90B10, 90C30, 90C35, 90C90, [MATH.MATH-OC]Mathematics [math]/Optimization and Control [math.OC], gas networks, Software, Information Systems
Abstract

We show that the feasibility of a booking in the European entry-exit gas market can be decided in polynomial time on single-cycle networks that are passive, i.e. do not contain controllable elements. The feasibility of a booking can be characterized by solving polynomially many nonlinear potential-based flow models for computing so-called potential-difference maximizing load flow scenarios. We thus analyze the structure of these models and exploit both the cyclic graph structure as well as specific properties of potential-based flows. This enables us to solve the decision variant of the nonlinear potential-difference maximization by reducing it to a system of polynomials of constant dimension that is independent of the cycle's size. This system of fixed dimension can be handled with tools from real algebraic geometry to derive a polynomial-time algorithm. The characterization in terms of potential-difference maximizing load flow scenarios then leads to a polynomial-time algorithm for deciding the feasibility of a booking. Our theoretical results extend the existing knowledge about the complexity of deciding the feasibility of bookings from trees to single-cycle networks., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2021

49. Thermal conductivity of benzothieno-benzothiophene derivatives at the nanoscale

Author

Claudio Melis, Vincent Lemaur, Alexandre Vercouter, Rémy Jouclas, Guillaume Schweicher, Yves Geerts, Stéphane Lenfant, Aleandro Antidormi, David Guerin, Magatte N. Gueye, Jérôme Cornil, Dominique Vuillaume, National Fund for Scientific Research (Belgium), Fonds de la Recherche Scientifique (Fédération Wallonie-Bruxelles), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), University of Mons [Belgium] (UMONS), Université libre de Bruxelles (ULB), Centrale de Micro Nano Fabrication - IEMN (CMNF - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Nanostructures, nanoComponents & Molecules - IEMN (NCM - IEMN), Catalan Institute of Nanoscience and Nanotechnology (ICN2), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Barcelona Institute of Science and Technology (BIST), International Solvay Institutes for Physics and Chemistry [Brussels], Vrije Universiteit Brussel (VUB)-Université libre de Bruxelles (ULB), Dipartimento di Fisica, Universita di Cagliari (Dipartimento di Fisica, Universita di Cagliari), Universita degli Studi di Cagliari [Cagliari], PCMP PCP, Renatech Network, ANR-16-CE05-0029,Harvesters,Récuperer l'energie thermique de l'environnement à l'aide d'un générateur thermoélectrique polymère pour alimenter un capteur autonome(2016), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université libre de Bruxelles (ULB)-Vrije Universiteit Brussel (VUB), and Università degli Studi di Cagliari = University of Cagliari (UniCa)

Subjects
Scanning thermal microscopy, Yield (engineering), Materials science, Thermal resistance, Analytical chemistry, FOS: Physical sciences, 02 engineering and technology, organic thermoelectricity, 010402 general chemistry, 01 natural sciences, [SPI]Engineering Sciences [physics], chemistry.chemical_compound, Molecular dynamics, Thermal conductivity, Thermal conductance, Mesoscale and Nanoscale Physics (cond-mat.mes-hall), Benzothiophene derivatives, Chimie, General Materials Science, [PHYS.COND]Physics [physics]/Condensed Matter [cond-mat], Anisotropy, Nanoscopic scale, [PHYS]Physics [physics], thermal conducHvity, Condensed Matter - Mesoscale and Nanoscale Physics, organic semiconductor, Physique, Approach to equilibrium, Benzothiophene, Crystalline directions, 021001 nanoscience & nanotechnology, scanning thermal microscopy (SThM), Thickness dependence, 0104 chemical sciences, Anisotropy factor, Molecular dynamics calculation, chemistry, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], approach to equilibrium molecular dynamics (AEMD), 0210 nano-technology
Abstract

We study by scanning thermal microscopy the nanoscale thermal conductance of films (40-400 nm thick) of [1]benzothieno[3,2-b][1]benzothiophene (BTBT) and 2,7-dioctyl[1]benzothieno[3,2-b][1]benzothiophene (C8-BTBT-C8). We demonstrate that the out-of-plane thermal conductivity is significant along the interlayer direction, larger for BTBT (0.63 ± 0.12 W m-1 K-1) compared to C8-BTBT-C8 (0.25 ± 0.13 W m-1 K-1). These results are supported by molecular dynamics calculations (approach to equilibrium molecular dynamics method) performed on the corresponding molecular crystals. The calculations point to significant thermal conductivity (3D-like) values along the 3 crystalline directions, with anisotropy factors between the crystalline directions below 1.8 for BTBT and below 2.8 for C8-BTBT-C8, in deep contrast with the charge transport properties featuring a two-dimensional character for these materials. In agreement with the experiments, the calculations yield larger values in BTBT compared to C8-BTBT-C8 (0.6-1.3 W m-1 K-1versus 0.3-0.7 W m-1 K-1, respectively). The weak thickness dependence of the nanoscale thermal resistance is in agreement with a simple analytical model. This journal is, M. G., D. G., S. L. and D. V. thank the ANR for financial support (ANR-16-CE05-0029). Y. G. is thankful to the Belgian National Fund for Scientific Research (FNRS) for financial support through research projects BTBT (# 2.4565.11), Phasetrans (# T.0058.14), Pi-Fast (# T.0072.18), 2D to 3D (# 30489208), and DIFFRA (# U.G001.19). Financial supports from the French Community of Belgian (ARC # 20061) is also acknowledged. G. S. acknowledges postdoctoral fellowship support from the FNRS. The work in the Laboratory for Chemistry of Novel Materials was supported by the Consortium des Équipements de Calcul Intensif (CÉCI), funded by the Fonds de la Recherche Scientifique de Belgique (F.R.S.-FNRS) under grant # 2.5020.11. J.C. and A.V. are FNRS research fellows.

Published
2021

50. Metastatic colorectal cancer cells maintain the TGFβ program and use TGFBI to fuel angiogenesis

Author

Akeila Bellahcene, Roberto Ronca, Guillaume Belthier, Patrick Balaguer, Simon Lacroix, Paola Chiodelli, Gaetan Van Simaeys, Andrei Turtoi, Barbara Chiavarina, Julie Pannequin, Olivier Detry, Guy Jerusalem, Serge Goldman, Stéphanie Gofflot, Arnaud Blomme, Eric Fabbrizio, Sara Rezzola, Gilles Doumont, Ambre Giguelay, Bilguun Erkhem-Ochir, Takehiko Yokobori, Vincent Castronovo, Brunella Costanza, Philippe Delvenne, Vincent Cavaillès, Emmanuel Di Valentin, Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), GIGA [Université Liège], Université de Liège, University of Brescia, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Center for Microscopy and Molecular Imaging (IBMM - CMMI), Université libre de Bruxelles (ULB), Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Gunma University, Centre Hospitalier Universitaire de Liège (CHU-Liège), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and UM, Faculté de Médecine

Subjects
0301 basic medicine, Colorectal Neoplasms -- blood supply -- metabolism -- pathology, Colorectal cancer, Angiogenesis, Endothelial cells, Cell, Medicine (miscellaneous), Apoptosis, alternative TGFβ signaling, Liver Neoplasms -- blood supply -- metabolism -- secondary, Liver metastases, Mice, 0302 clinical medicine, Circulating tumor cell, Cell Movement, Transforming Growth Factor beta, Tumor Cells, Cultured, Medicine, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Extracellular Matrix Proteins, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Neovascularization, Pathologic, Liver Neoplasms, Sciences bio-médicales et agricoles, Prognosis, endothelial cells, 3. Good health, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Neovascularization, Pathologic -- metabolism -- pathology, 030220 oncology & carcinogenesis, Signal transduction, Colorectal Neoplasms, Signal Transduction, Research Paper, Extracellular Matrix Proteins -- genetics -- metabolism, Alternative TGFβ signaling, Stromal cell, 03 medical and health sciences, Settore MED/04 - PATOLOGIA GENERALE, Biomarkers, Tumor, Animals, Humans, Cell Proliferation, business.industry, Transforming Growth Factor beta -- genetics -- metabolism, medicine.disease, Xenograft Model Antitumor Assays, eye diseases, 030104 developmental biology, Cancer cell, Cancer research, Sciences pharmaceutiques, business, liver metastases, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Biomarkers, Tumor -- genetics -- metabolism, TGFBI
Abstract

Colorectal cancer (CRC) cells are traditionally considered unresponsive to TGFβ due to mutations in the receptors and/or downstream signaling molecules. TGFβ influences CRC cells only indirectly via stromal cells, such as cancer-associated fibroblasts. However, CRC cell ability to directly respond to TGFβ currently remains unexplored. This represents a missed opportunity for diagnostic and therapeutic interventions. Methods: We examined whether cancer cells from primary CRC and liver metastases respond to TGFβ by inducing TGFβ-induced protein ig-h3 (TGFBI) expression, and the contribution of canonical and non-canonical TGFβ signaling pathways to this effect. We then investigated in vitro and in vivo TGFBI impact on metastasis formation and angiogenesis. Using patient serum samples and an orthotopic mouse model of CRC liver metastases we assessed the diagnostic/tumor targeting value of novel antibodies against TGFBI. Results: Metastatic CRC cells, such as circulating tumor cells, directly respond to TGFβ. These cells were characterized by the absence of TGFβ receptor mutations and the frequent presence of p53 mutations. The pro-tumorigenic program orchestrated by TGFβ in CRC cells was mediated through TGFBI, the expression of which was positively regulated by non-canonical TGFβ signaling cascades. TGFBI inhibition was sufficient to significantly reduce liver metastasis formation in vivo. Moreover, TGFBI pro-tumorigenic function was linked to its ability to stimulate angiogenesis. TGFBI levels were higher in serum samples from untreated patients with CRC than in patients who were receiving chemotherapy. A radiolabeled anti-TGFBI antibody selectively targeted metastatic lesions in vivo, underscoring its diagnostic and therapeutic potential. Conclusions: TGFβ signaling in CRC cells directly contributes to their metastatic potential and stromal cell-independence. Proteins downstream of activated TGFβ, such as TGFBI, represent novel diagnostic and therapeutic targets for more specific anti-metastatic therapies., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results