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1. Overview of the Third International Workshop on Swine Leukocyte Differentiation Antigens

Author

Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Álvarez, Belén, Alonso, Fernando, Bailey, M., Bianchi, A. T. J., Boersma, W. J. A., Chen, Zhang, Davis, William, Domínguez, Javier, Engelhardt, H., Ezquerra Martínez, Ángel, Grosmaire, L. S., Hamilton, M. J., Hollemweguer, E., Huang, C. A., Khanna, K. V., Kuebart, G., Lackovic, G., Ledbetter, J. A., Lee, Rogan, Llanes, D., Lunney, J. K., McCullough, Kenneth C., Molitor, T., Nielsen, Jens, Niewold, T. A., Pescovitz, M. D., Pérez de Lastra, José Manuel, Rehakova, Z., Salmon, H., Schnitzlein, W. M., Seebach, J., Simón, Alicia, Sinkora, J., Sinkora, M., Stokes, Christopher, Summerfield, A., Sver, L., Thacker, E., Valpotic, I., Yang, Huaizhi, Zuckermann, F., Zwart, R. J., Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Álvarez, Belén, Alonso, Fernando, Bailey, M., Bianchi, A. T. J., Boersma, W. J. A., Chen, Zhang, Davis, William, Domínguez, Javier, Engelhardt, H., Ezquerra Martínez, Ángel, Grosmaire, L. S., Hamilton, M. J., Hollemweguer, E., Huang, C. A., Khanna, K. V., Kuebart, G., Lackovic, G., Ledbetter, J. A., Lee, Rogan, Llanes, D., Lunney, J. K., McCullough, Kenneth C., Molitor, T., Nielsen, Jens, Niewold, T. A., Pescovitz, M. D., Pérez de Lastra, José Manuel, Rehakova, Z., Salmon, H., Schnitzlein, W. M., Seebach, J., Simón, Alicia, Sinkora, J., Sinkora, M., Stokes, Christopher, Summerfield, A., Sver, L., Thacker, E., Valpotic, I., Yang, Huaizhi, Zuckermann, F., and Zwart, R. J.

Abstract

The aim of the Third International Workshop on Swine Leukocyte Differentiation Antigens (CD workshop), supported by the Veterinary Immunology Committee (VIC) of the International Union of Immunological Societies (IUIS), was to standardize the assignment of monoclonal antibodies (mAb) reactive with porcine leukocyte differentiation antigens and to define new antibody clusters, using nomenclature in accordance with human and ruminant CD nomenclature, as agreed at the summary meeting of the Second International Swine CD Workshop in Davis, 1995 only mAb with proven reactivity for the orthologous porcine gene product or cross-reactivity for the human gene products, were given the full CD nomenclature, all other allocations were prefixed with 'w'.As in previous workshops, the overall organization was entrusted to the chair and first author, with support by the chair of the previous workshop and second author.In addition to the existing 26 pig leukocyte CD/SWC determinants established in previous workshops, this workshop established/confirmed another 11 CDs for pig leukocytes, identified by a total of 21 mAb CD11R1 (2 mAb), CD11R2 (1 mAb), CD11R3 (4 mAb), wCD40 (1 mAb), wCD46 (4 mAb), wCD47 (3 mAb), wCD49d (1 mAb), CD61 (1 mAb), wCD92 (1 mAb), wCD93 (1 mAb) and CD163 (2 mAb). Copyright © 2001 Elsevier Science B.V.

Published
2001

2. Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens

Author

Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Chen, Zhang, Huang, C. A., Simón, Alicia, Seebach, J., Boersma, W. J. A., Zwart, R. J., Niewold, T. A., Thacker, E., Llanes, D., Pérez de Lastra, José Manuel, Engelhardt, H., Ezquerra Martínez, Ángel, Alonso, Fernando, Domínguez, Javier, Ledbetter, J. A., Grosmaire, L. S., Lee, Rogan, Nielsen, Jens, Salmon, H., Valpotic, I., Sver, L., Lackovic, G., Summerfield, A., Khanna, K. V., Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Chen, Zhang, Huang, C. A., Simón, Alicia, Seebach, J., Boersma, W. J. A., Zwart, R. J., Niewold, T. A., Thacker, E., Llanes, D., Pérez de Lastra, José Manuel, Engelhardt, H., Ezquerra Martínez, Ángel, Alonso, Fernando, Domínguez, Javier, Ledbetter, J. A., Grosmaire, L. S., Lee, Rogan, Nielsen, Jens, Salmon, H., Valpotic, I., Sver, L., Lackovic, G., Summerfield, A., and Khanna, K. V.

Abstract

The reactivity of 155 monoclonal antibodies submitted to the Third International Workshop on Swine Leukocyte Differentiation Antigens, together with 41 internal standards, was analysed by flow cytometry on 29 different pig cell targets as well as two human cell targets as a means of establishing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without further gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in two-colour flow cytometry. The CD or SWC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leucocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specificity of known standards which clustered in a group. Copyright © 2001 Elsevier Science B.V.

Published
2001

3. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens:results from the 2. International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., Saalmüller, A., Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., and Saalmüller, A.

Published
1998

4. Summary of workshop findings for antibodies reacting with porcine T- cells and activation antigens Results from the Second International Swine CD Workshop

Author

Lee, Rogan [0000-0001-8364-9515], Pescovitz, M. D., Book, B. K., Aasted, B., Domínguez, Javier, Ezquerra Martínez, Ángel, Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., Zuckermann, F., Saalmüller, A., Lee, Rogan [0000-0001-8364-9515], Pescovitz, M. D., Book, B. K., Aasted, B., Domínguez, Javier, Ezquerra Martínez, Ángel, Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., Zuckermann, F., and Saalmüller, A.

Abstract

After initial evaluation of the 176 new and 19 control monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mAb were further analyzed using flow cytometry on whole blood lymphocytes, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, broncho-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4, and CD8. Finally, the molecular weights of the target antigens were characterized when possible. As a result of these analyses, 23 mAb were distributed into 7 CD clusters. Newly confirmed mAb assigments included two CD2; one CD4; two CD5; one 7 CD and one wCD25. Three new mAb were found that reacted with wCD8, one of which defined a new epitope, wCD8c. For the first time, mAb against porcine CD3 were identified, including 6 mAb that reacted with three different epitopes. Several new mAb reacted with antigens whose expression varied depending on the activation state of the test cell. These will require further characterization in order to assign a CD number.

Published
1998

5. First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm.

Author

Bošnjak I, Bielen A, Babić S, Sver L, Popović NT, Strunjak-Perović I, Což-Rakovac R, and Klobučar RS

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 antagonists & inhibitors, ATP Binding Cassette Transporter, Subfamily B, Member 1 chemistry, Amino Acid Sequence, Animals, Base Sequence, DNA, Complementary chemistry, Gene Expression, Molecular Sequence Data, Oligochaeta chemistry, Rhodamine 123 analysis, Rhodamines chemistry, ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics, Drug Resistance, Multiple genetics, Oligochaeta genetics, Proteins chemistry, Xenobiotics pharmacology
Abstract

Multixenobiotic resistance (MXR) is an important mechanism of cellular efflux mediated by ATP binding cassette (ABC) transporters that bind and actively remove toxic substrates from the cell. This study was the first to identify ABC transporter P-glycoprotein (P-gp/ABCB1) as a representative of the MXR phenotype in earthworm (Eisenia fetida). The identified partial cDNA sequence of ABCB1 overlapped with ABCB1 homologues of other organisms from 58.5 % to 72.5 %. We also studied the effect of five modulators (verapamil, cyclosporine A, MK571, probenecid, and orthovanadate) on the earthworm's MXR activity by measuring the accumulation of model substrates rhodamine B and rhodamine 123 in whole body tissue of the adult earthworm. MK571, orthovanadate, and verapamil significantly inhibited MXR activity, and rhodamine 123 turned out to better reflect MXR activity in that species than rhodamine B. Our results show that E. fetida can serve well as a test organism for environmental pollutants that inhibit MXR activity.

Published
2014
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6. Effects of local administration of propolis and its polyphenolic compounds on tumor formation and growth.

Author

Orsolić N, Terzić S, Mihaljević Z, Sver L, and Basić I

Subjects
Animals, Chromatography, High Pressure Liquid, DNA Replication drug effects, Female, Humans, Male, Mice, Mice, Inbred CBA, Polyphenols, Propolis administration & dosage, Cell Division drug effects, Flavonoids pharmacology, Mammary Neoplasms, Experimental pathology, Phenols pharmacology, Propolis pharmacology
Abstract

Many dietary constituents are chemopreventive in animal models, and experiments with cultured cells are revealing various potential mechanisms of action. Compounds classified as blocking agents can prevent, or greatly reduce, initiation of carcinogenesis, or suppressing agents can act on cell proliferation. Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE), members of the polyphenolic compounds, are present in high concentrations in medicinal plants and propolis, a natural beehive product. A water-soluble extract of propolis (WSDP) and two components of propolis, CA and CAPE were investigated for direct antitumor activity in vivo and in vitro. The local presence of CA and CAPE in the tissue caused a significant delay in tumor formation and increased life span 29.30 to 51.73%, respectively. CA and CAPE, but not WSDP, significantly suppressed human HeLa cervical carcinoma cell proliferation in vitro. Based on these results, we postulate that the antitumor activity of polyphenolic compounds includes direct cytotoxic effects on tumor cells.

Published
2005
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7. Leukocyte subsets and specific antibodies in pigs vaccinated with a classical swine fever subunit (E2) vaccine and the attenuated ORF virus strain D1701.

Author

Terzić S, Jemersić L, Lojkić M, Sver L, Valpotić I, Orsolić N, Humski A, and Cvetnić Z

Subjects
Animals, Antibodies, Viral analysis, Classical Swine Fever Virus classification, Flow Cytometry veterinary, Leukocyte Count veterinary, Lymphocyte Subsets, Orf virus classification, Swine, Treatment Outcome, Vaccines, Attenuated immunology, Classical Swine Fever immunology, Classical Swine Fever prevention & control, Classical Swine Fever Virus immunology, Orf virus immunology, Vaccination veterinary, Viral Vaccines immunology
Abstract

Total white blood cell (WBC) counts and percentages of CD4a+, CD8a+, CD5a+, CD45RA+, CD45RC+, wCD21+ and SWC3a+ cells in the peripheral blood of pigs were analysed in this study. Blood samples were collected before and on days 4, 10, 21 and 28 after vaccination. Group 1 pigs were vaccinated with a subunit E2 vaccine (gp E2 32 microg/dose), and Group 2 received a subunit vaccine combined with an attenuated ORF virus strain D1701 10(6.45) TCID50/dose. Control pigs received a placebo. The total WBC count and percentage of particular cell types were within the normal range in vaccinated and control pigs. Although the mechanism of attenuated ORF virus activity is not clear, changes were observed in CD4a+, CD5a+, CD8a+, CD45RA+ and CD45RC+ cells in pigs that received the combination of a subunit vaccine and ORF virus. However, the percentage of wCD21+ and SWC3a+ did not differ significantly from that recorded in pigs given only the subunit vaccine. At days 4 and 10 the number of pigs positive to E2 antibodies was higher in the group that received the subunit vaccine and ORF virus than in pigs vaccinated with the subunit vaccine only. A higher percentage of memory cells (CD45RC+) as well as Th and Tc lymphocytes in pigs that received the ORF virus and the subunit vaccine could be ascribed to a nonspecific influence of the ORF virus on the development (through cognate interactions between T and B cells) and the duration (presumed according to the finding of the clonal expression of memory cells) of humoral immunity (assessed by a higher number of seropositive pigs in this group). This seems likely since the proportion of these cells was found to be lower in the pigs that received E2 vaccine only.

Published
2004
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