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Gitishree Das,1 Sujin Seo,2 In-Jun Yang,3 Ly Thi Huong Nguyen,3 Han-Seung Shin,2 Jayanta Kumar Patra1 1Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Goyangsi, Republic of Korea; 2Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi, Republic of Korea; 3Department of Physiology, College of Korean Medicine, Dongguk University, Gyeongju, Republic of KoreaCorrespondence: Jayanta Kumar Patra, Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Goyangsi, 10326, Republic of Korea, Tel +82-31-961-5625, Email jkpatra@dongguk.eduIntroduction: A number of biological wastes and factory waste materials have been tested recently for the eco-friendly biosynthesis of nanoparticles. Sericin protein (SSP) is usually removed from the silk cocoon during the degumming process in the process of making the silk, and this sericin protein is normally thrown away by the sericulture industries as waste materials. It is found that this sericin protein possesses a number of biological properties.Methods: Considering this, in the present study, an effort has been made to biosynthesize gold nanoparticles (SSP-AuNPs) using the waste sericin solution as the reducing and capping agent and investigate its biopotential in terms of its wound healing, antioxidant and antibacterial activities.Results: The synthesis of SSP-AuNPs was perceived by the visual color change and confirmed by UV-Vis spectroscopy with absorption maxima at 522 nm. Further characterization of SSP-AuNPs was done by TEM, EDS, XRD, FTIR, DLS, zeta potential, TGA, AFM, etc. The size of SSP-AuNPs was found out to be 54.82 nm as per the particle size analyzer and the zeta potential is − 19.8 mV. The SSP-AuNPs displayed promising wound healing potential of 70.96 and 69.76% wound closure rate at 5 and 10 μg/mL respectively as compared to 74.91% by the Centella asiatica taken as a positive control. It also exhibited promising antioxidant p

Gitishree Das,1,2 Han-Seung Shin,3 Jayanta Kumar Patra1,2 1Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Gyeonggi-do, Republic of Korea; 2Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Gyeonggi-do, Republic of Korea; 3Department of Food Science and Biotechnology, Dongguk University‐Seoul, Gyeonggi‐do, Republic of KoreaCorrespondence: Jayanta Kumar Patra Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Gyeonggi-do, 10326, Republic of Korea Tel +82-31-961-5625 Email jkpatra.cet@gmail.comPurpose: Due to the biomedical applications universally, the Ag nanoparticles are one of the most commonly investigated nanoparticles (NPs). Curly kale (BroL) leaves contain numerous beneficial nutrients and phytochemicals. The aim of the current study is the fabrication of the Ag nanoparticles using the extracts of curly kale and to investigate their biological potentials.Methods: The characterization of the generated BroLAgNPs was done through UV-Vis spectro study, Fourier-transform infrared spectro study, scanning electron microscope analysis, energy-dispersive X-ray study, distribution of size and zeta potential investigation, and X-ray powder diffraction study, and their biological effects were evaluated by antidiabetic, antioxidant, antibacterial and cytotoxicity effect.Results: BroL-Ag nanoparticle displayed surface plasmon resonance at 432 nm. The Zeta potential of BroL (− 26.6) AgNPs displayed a highly negative charge. In antidiabetic assay, BroL-AgNPs was highly effective with IC50 value 2.29 μg/mL at 1.0 μg/mL concentration. In cytotoxicity assay, BroL-AgNPs displayed strong activity at 10.0 μg/mL concentration. It showed inhibitory action against three food-borne pathogenic bacteria (9.29– 11.44 mm inhibition zone) and displayed moderate antioxidant potential.Conclusion: This study as a whole report an eco-friendly green synthesis of AgNPs using leafy vegetable

Gitishree Das,1 Han-Seung Shin,2 Jayanta Kumar Patra1 1Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Gyeonggi-do, 10326, Republic of Korea; 2Department of Food Science and Biotechnology, Dongguk University‐Seoul, Gyeonggi‐do, 10326, Republic of KoreaCorrespondence: Jayanta Kumar Patra, Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Gyeonggi-do, 10326, Republic of Korea, Email jkpatra@dongguk.eduIntroduction: Nowadays, in nanotechnology and material science, biosynthesis of the metal nanoparticle is a promising approach.Methods: In the current research, the extract of the Korean Ueong dry root (BdkR), which belongs to the Asteraceae family, was used as a reducing and capping agent, for the green synthesis of the BdkR-Ag nanoparticles in a cost-effective and highly efficient manner. In this study for the reaction measures, UV-Vis spectroscopy was applied. SEM, EDX, FTIR, XRD, mean size distribution, and zeta potential were used for the characterization of the green synthesized BdkR-AgNPs. In the beginning, the primary phytochemical screening of BdkR extract was estimated and the cytotoxicity, antidiabetic, antioxidant, and antibacterial activities of the green synthesized BdkR-AgNPs were evaluated.Results: According to the results, the BdkR extract is rich in various phytochemicals and the generated AgNPs were crystalline in nature. The surface plasmon resonance value of the BdkR-AgNPs was 444 nm confirming the synthesis of AgNPs. The BdkR-AgNPs displayed four clear diffraction peaks at 2 theta angles (38.22); (46.15); (64.88); (76.83), respectively, which are equivalent to (111), (200), (220) and (311). The obtained nanoparticles have a zeta potential of − 17.0 mV. Furthermore, the generated BdkR-AgNPs exhibited considerable antidiabetic effect in terms of the inhibition of α-glucosidase with a maximum inhibition value of 95.41% at 5.0 μg/mL and more than 86% inhibition at 2.5 μg/mL and the estimated I