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The present study was aimed to develop a novel, simple, accurate, precise, robust and economic UV spectrophotometric method for the detection and quantification of vancomycin in bulk and its formulation. Quantification was carried at absorption maxima 280.50 nm using 0.01M Sodium hydroxide as diluent. The developed method was validated with accordance to ICH Q2 (R1) guidelines. Beer’s law was obeyed in the concentration range of 40-240 μg/mL with correlation coefficient of 0.9991. The sensitivity was checked as the limit of detection and limit of quantification which were found to be 1.6 and 4.8 μg/mL respectively. Further studies of the accuracy and precision of the proposed method were performed using standard addition method; the mean quantitative recovery of such studies were found to be in the range 99.39 ± 0.41 to 101.24 ± 0.89 with RSD ≤ 2.0%. From the results it can be concluded that the developed method was specific, selective, precise and robust. This method could be successfully applied for analysis of vancomycin in its formulation.

Objective: This study was aimed to develop a novel, selective, and sensitive reverse phase high performance liquid chromatography method for the detection and quantification of aripiprazole in pure form as well as in its pharmaceutical formulation.Methods: The chromatography was carried out on C18 Phenomenex Luna (250 × 4.6 mm × 5 μm) column using a mobile phase of 0.1% v/v orthophosphoric acid (pH 3.0), acetonitrile and methanol in the ratio of 40:50:10 (% v/v). The isocratic elution program was adjusted to 1.0 ml/minutes flow rate with 20 μl injection volume. The eluted components were monitored at 254 nm. The ambient column oven temperature was maintained.Results: The developed method was validated statistically with respect to linearity, range, precision, accuracy, specificity, robustness, ruggedness, detection and quantification limits and also subjected to various stress conditions such as acidic and alkaline hydrolysis, oxidation, photolysis, and thermal degradation. The method showed linearity across the concentration range of 10.0-60 μg/ml.Conclusion: The developed method is specific, precise, accurate, robust and stability indicating which can be successfully applied for routine analysis, quality control analysis and also suitable for stability analysis of assay of aripiprazole in pure form and its formulation as per the regulatory requirements.Keywords: Aripiprazole, Reverse phase high performance liquid chromatography, Stress condition.