196 results on '"Sang Woo Han"'
Search Results
2. Productive efficiency and trade opportunities for Kazakhstan dairy farms
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Sang Woo Han, Song Soo Lim, and Aida Balkibayeva
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data envelopment analysis ,production efficiency ,kazakhstan dairy industry ,belt and road initiative ,Agriculture - Abstract
This study analyses the productive efficiency of dairy farms in Kazakhstan and suggests export implications by the expansion of trade networks and the participation of global value chains. As the world’s largest landlocked country and ninth largest area in the world, Kazakhstan has often been considered to have vast potential to produce and export dairy products. The greater openness of markets and improved geostrategic circumstances with the latest rail link between China and Europe are expected to strengthen Kazakhstan’s trade opportunities with the rest of the world. Despite these positive prospects, few empirical studies have examined the export potential of the country’s dairy products. To bridge this gap, this study surveys 23 dairy farms across nine oblasts in Kazakhstan and performs a data envelopment analysis with milk production as the output variable and feed, labour, and capital as the input variables. The estimation results indicate that Kazakh dairy farms could reduce input use by up to 70% under the most efficient system. A dichotomy of productive efficiency among large and capital-intensive versus small-scale family farms suggests that the country should promote inclusiveness through sharing knowledge and best practices within the industry.
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- 2021
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3. Communicating Tubular Esophageal Duplication Combined with Bronchoesophageal Fistula
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Ju Hwan Kim, Chang-Il Kwon, Ji Young Rho, Sang Woo Han, Ji Su Kim, Suk Pyo Shin, Ga Won Song, and Ki Baik Hahm
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Esophageal duplication ,Bronchoesophageal fistula ,Bronchial fistula ,Esophageal fistula ,Internal medicine ,RC31-1245 ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Esophageal duplication (ED) is rarely diagnosed in adults and is usually asymptomatic. Especially, ED that is connected to the esophagus through a tubular communication and combined with bronchoesophageal fistula (BEF) is extremely rare and has never been reported in the English literature. This condition is very difficult to diagnose. Although some combinations of several modalities, such as upper gastrointestinal endoscopy, esophagography, computed tomography, magnetic resonance imaging, and endoscopic ultrasonography, can be used for the diagnosis, the results might be inconclusive. Here, we report on a patient with communicating tubular ED that was incidentally diagnosed on the basis of endoscopy and esophagography during the postoperational evaluation of BEF.
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- 2016
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4. Miliary Brain Tuberculomas
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Sang Won Ha and Sang Woo Han
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Neurology. Diseases of the nervous system ,RC346-429 - Published
- 2017
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5. Rapidly Progressed Spinal Dural Arteriovenous Fistula Mimics Transverse Myelitis
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Sul Ki Lee, Sang Woo Han, In-Ha Whang, Sang Won Ha, Seung Min Kim, In Joong Kim, and Wan Tae Kim
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Neurology. Diseases of the nervous system ,RC346-429 - Published
- 2015
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6. Depression and self-concept in girls with perception of pubertal onset
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Ji Hyeon Yang, Sang Woo Han, Chan Woo Yeom, Yong Jun Park, Wha Su Choi, Ji Young Seo, and Young Jin Koo
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Puberty ,Sexual development ,Depression ,Self concept ,Pediatrics ,RJ1-570 - Abstract
PurposeEarly pubertal timing in girls is associated with psychological and behavioral problems. This study aimed to evaluate the psychological features of girls who perceived breast development beginning by analyzing their depression levels and self-concept.MethodsFrom March 2007 to December 2012, 93 girls were enrolled and assigned to a pre-8 (younger than 8 years, n=43) or post-8 (8 years and older, n=50) group according to the age at onset of perceived breast development, and their height, body weight, body mass index, bone age (BA), Tanner stage, and luteinizing hormone and follicle-stimulating hormone levels were examined. We investigated their psychological state with the Korean Children's Depression Inventory (CDI) and Piers-Harris Children's Self-Concept Scale (PHCSC) to evaluate depression levels and self-concept, respectively.ResultsThe pre-8 group had a significantly greater height standard deviation score, (0.5±1.01 vs. 0.11±0.86, P = 0.048) and more advanced BA (2.07±1.02 years vs. 1.40±0.98 years, P = 0.004) compared to the post-8 group. There were no statistically significant intergroup differences for the CDI and PHCSC scores; however, the pre-8 group scored higher than the post-8 group in the physical appearance and attributes domain of the PHCSC (9.93±2.57 vs. 8.52±3.03, P = 0.017).ConclusionThe timing of perceived breast development among girls who thought puberty to begin did not affect depression levels and self-concept. There was no correlation between Tanner stage and depression levels and self-concept despite the perception of pubertal onset. The pre-8 group had a more positive view of their physical appearance than the post-8 group.
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- 2013
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7. Chronic Cerebral Paragonimiasis: Neuroimage Findings Provided a Clue for Paragonimiasis
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Sang Woo Han, In Ha Hwang, Seung Min Kim, and Sang Won Ha
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Neurology. Diseases of the nervous system ,RC346-429 - Published
- 2015
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8. Reprogramming biocatalytic futile cycles through computational engineering of stereochemical promiscuity to create an amine racemase
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Sang-Woo Han, Youngho Jang, Jihyun Kook, Jeesu Jang, and Jong-Shik Shin
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Science - Abstract
Abstract Repurposing the intrinsic properties of natural enzymes can offer a viable solution to current synthetic challenges through the development of novel biocatalytic processes. Although amino acid racemases are ubiquitous in living organisms, an amine racemase (AR) has not yet been discovered despite its synthetic potential for producing chiral amines. Here, we report the creation of an AR based on the serendipitous discovery that amine transaminases (ATAs) can perform stereoinversion of 2-aminobutane. Kinetic modeling revealed that the unexpected off-pathway activity results from stereochemically promiscuous futile cycles due to incomplete stereoselectivity for 2-aminobutane. This finding motivated us to engineer an S-selective ATA through in silico alanine scanning and empirical combinatorial mutations, creating an AR with broad substrate specificity. The resulting AR, carrying double point mutations, enables the racemization of both enantiomers of diverse chiral amines in the presence of a cognate ketone. This strategy may be generally applicable to a wide range of transaminases, paving the way for the development of new-to-nature racemases.
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- 2024
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9. Urgent craniectomy for subdural hematoma in the 8-month-old infant with unrecognized hemophilia B
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Jong Bun Kim, Hyun-Ju Jung, Kyong Shil Im, Sang Woo Han, and Sang-Hun Lee
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Anesthesiology ,RD78.3-87.3 - Published
- 2013
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10. Modification of Hybrid Receptor Model for Atmospheric Fine Particles (PM2.5) in 2020 Daejeon, Korea, Using an ACERWT Model
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Sang-woo Han, Hung-soo Joo, Kyoung-chan Kim, Jin-sik Cho, Kwang-joo Moon, and Jin-seok Han
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PM2.5 ,positive matrix factorization ,emission source ,advanced concentration emission and retention time-weighted trajectory ,hybrid receptor model ,Meteorology. Climatology ,QC851-999 - Abstract
Hybrid receptor models overestimate the contribution of background areas (no specific emission sources), like the Yellow Sea in Korea. This study aimed to improve model performances using Advanced Concentration Emission and Retention Time Weighted Trajectory (ACERWT). ACERWT was combined with a positive matrix factorization (PMF), back trajectory, and Regional Emission Inventory in Asia (REAS). The PMF receptor model used one year of data from Korea’s Central Air Environment Research Center. In the PMF receptor model, eight sources (dust/soil, secondary nitrate, biomass burning, vehicles, secondary sulfate, industry, coal combustion and sea salt) influenced PM2.5 pollution at the receptor site (Daejeon, Korea). Secondary sulfate was the most dominant source, followed by secondary nitrate and vehicle sources. ACERWT results showed high contributions from China, Japan, and Korean regions, while the contribution from the Yellow Sea was significantly lower. Several regions, such as the eastern and south-eastern areas of China, the southern area of Taiwan, the western area of Tokyo, and the central area of Korea, showed high contributions due to large-scale emission facilities and industrial complexes. In this study, the ACERWT model significantly improved its performance regarding regional contributions to PM2.5 pollution at the receptor site.
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- 2024
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11. Advancements in the Application of Ribosomally Synthesized and Post-Translationally Modified Peptides (RiPPs)
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Sang-Woo Han and Hyung-Sik Won
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bioactive peptides ,genetic engineering ,heterologous expression ,high-throughput screening ,RiPPs ,synthetic biology ,Microbiology ,QR1-502 - Abstract
Ribosomally synthesized and post-translationally modified peptides (RiPPs) represent a significant potential for novel therapeutic applications because of their bioactive properties, stability, and specificity. RiPPs are synthesized on ribosomes, followed by intricate post-translational modifications (PTMs), crucial for their diverse structures and functions. PTMs, such as cyclization, methylation, and proteolysis, play crucial roles in enhancing RiPP stability and bioactivity. Advances in synthetic biology and bioinformatics have significantly advanced the field, introducing new methods for RiPP production and engineering. These methods encompass strategies for heterologous expression, genetic refactoring, and exploiting the substrate tolerance of tailoring enzymes to create novel RiPP analogs with improved or entirely new functions. Furthermore, the introduction and implementation of cutting-edge screening methods, including mRNA display, surface display, and two-hybrid systems, have expedited the identification of RiPPs with significant pharmaceutical potential. This comprehensive review not only discusses the current advancements in RiPP research but also the promising opportunities that leveraging these bioactive peptides for therapeutic applications presents, illustrating the synergy between traditional biochemistry and contemporary synthetic biology and genetic engineering approaches.
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- 2024
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12. Emission Characteristics of Fine Particles in Relation to Precursor Gases in Agricultural Emission Sources: A Case Study of Dairy Barns
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Hung-Soo Joo, Sang-Woo Han, Jin-Seok Han, and Pius M. Ndegwa
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fine particle formation ,gaseous precursor ,emission rate ,loading rate ,correlation analysis ,Meteorology. Climatology ,QC851-999 - Abstract
Recently, precursor gases such as ammonia have sparked a growing interest in the secondary formation of particulate matter (PM). Most studies focus on urban areas and scientific data. Studies on precursor gases and PM emitted from agricultural sources are insufficient; thus, this paper presents a field monitoring study conducted from agricultural sources. To estimate the effect of precursor gases for PM2.5 from naturally ventilated dairy barns, correlation analyses were conducted using real-time monitoring data on the mass concentrations of PM2.5, NH3, SO2, NO2, and H2S and meteorological data. In addition to mass concentration, the emission and loading rates were used to closely analyze pollution status. The mass concentrations of PM2.5 and gaseous compounds did not correlate well, whereas the mass emission rates for PM2.5 and gaseous compounds (SO2, NH3, and NO2) correlated well because the unit of the emission rate reflected the ventilation factor. The correlation coefficients between PM2.5 and precursor gas emission rates ranged from 0.72 to 0.89 (R), with the SO2 emission rate exhibiting the highest correlation coefficient (R = 0.89). This correlation implies that SO2 from dairy farms is a dominant species among the gaseous precursors influencing the secondary formation of PM2.5; alternatively, SO2 and PM2.5 are produced from the same sources. The ambient PM2.5 loading rate and barn PM2.5 emission rate—estimated by multivariate linear regression using the gaseous independent variables NH3, SO2, and NO2—revealed high-correlation coefficients (0.60 and 0.92, respectively) with the measured data. At present, most studies investigating the precursor gases of PM in agricultural fields have focused on NH3; however, this study suggests that SO2 is a key factor in PM2.5 pollution. To elucidate the secondary formation of PM from precursor gases in agricultural sources, particulate ammonium, sulfate, nitrate, and chloride, which were not measured in this study, as well as oxidants and intermediates, should be considered in future research.
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- 2023
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13. GMA process development for pore-free zinc-coated steel sheet welding in automotive industry
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Sang-Woo Han, Hansol Kim, Geonho Lee, Seungcheol Shin, Jongho Jeon, Sangjun Han, Gyuyeol Bae, and Jungho Cho
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Control and Systems Engineering ,Mechanical Engineering ,Industrial and Manufacturing Engineering ,Software ,Computer Science Applications - Published
- 2023
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14. Orbital floor defect caused by invasive aspergillosis: a case report and literature review.
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Sang Woo Han, Min Woo Park, Sug Won Kim, Minseob Eom, Dong Hwan Kwon, Eun Jung Lee, and Jiye Kim
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ASPERGILLOSIS , *MYCOSES , *VORICONAZOLE - Abstract
Fungal sinusitis is relatively rare, but it has become more common in recent years. When fungal sinusitis invades the orbit, it can cause proptosis, chemosis, ophthalmoplegia, retroorbital pain, and vision impairment. We present a case of an extensive orbital floor defect due to invasive fungal sinusitis. A 62-year-old man with hypertension and a history of lung adenocarcinoma, presented with right-side facial pain and swelling. On admission, the serum glucose level was 347 mg/dL, and hemoglobin A1c was 11.4%. A computed tomography scan and a Waters’ view X-ray showed right maxillary sinusitis with an orbital floor defect. On hospital day 3, functional endoscopic sinus surgery was performed by the otorhinolaryngology team, and an aspergilloma in necrotic inflammatory exudate obtained during exploration. On hospital day 7, orbital floor reconstruction with a Medpor Titan surgical implant was done. In principle, the management of invasive sino-orbital fungal infection often begins with surgical debridement and local irrigation with an antifungal agent. Exceptionally, in this case, debridement and immediate orbital floor reconstruction were performed to prevent enophthalmos caused by the extensive orbital floor defect. The patient underwent orbital floor reconstruction and received intravenous and oral voriconazole. Despite orbital invasion, there were no ophthalmic symptoms or sequelae. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Simultaneous Analysis of Soluble and Insoluble Oxygen Contents in Al-Killed Steels of Various C Contents and Supersaturation Phenomena in the Steel
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Yong-Min Cho, Dong-Jun Lee, Hyun-Jin Cho, Wan-Yi Kim, Sang-Woo Han, and Youn-Bae Kang
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Mechanics of Materials ,Mechanical Engineering ,Materials Chemistry ,Metals and Alloys - Published
- 2022
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16. High-Temperature Static and Dynamic Characteristics of 4.2-kV GaN Super-Heterojunction p-n Diodes
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Mansura Sadek, Sang-Woo Han, Jianan Song, James C. Gallagher, Travis J. Anderson, and Rongming Chu
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Electrical and Electronic Engineering ,Electronic, Optical and Magnetic Materials - Published
- 2022
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17. 12.5 kV GaN Super-Heterojunction Schottky Barrier Diodes
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Suzanne E. Mohney, Mona A. Ebrish, Alex Molina, Rongming Chu, Mansura Sadek, Jianan Song, Sang-Woo Han, and Travis J. Anderson
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Materials science ,business.industry ,Schottky barrier ,Contact resistance ,Schottky diode ,Heterojunction ,Cathode ,Electronic, Optical and Magnetic Materials ,law.invention ,law ,Etching (microfabrication) ,Optoelectronics ,Breakdown voltage ,Electrical and Electronic Engineering ,business ,Diode - Abstract
This article reports GaN super-heterojunction Schottky barrier diodes (SHJ-SBDs) with substantially improved performance. Metal-2DEG sidewall n-ohmic contacts were deployed to achieve low contact resistance of $0.75~\Omega ~ \cdot ~mm$ , avoiding the risk of abnormally high contact resistance caused by inaccurate etch depth control. A pGaN notch formed near the cathode successfully eliminated excessive hole conduction caused by the sidewall n-ohmic contact. Isolation was improved by a high-energy Al implantation step. The resulting SHJ-SBD exhibited a breakdown voltage (BV) of ~12.5 kV and a specific resistance of 100.8 $\text{m}\Omega ~ \cdot ~cm^{2}$ .
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- 2021
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18. Sensory change and recovery of infraorbital area after zygomaticomaxillary and orbital floor fractures
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Sang Woo Han, Jeong Ho Kim, Sug Won Kim, Sung Hwa Kim, Dae Ryong Kang, and Jiye Kim
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Otorhinolaryngology ,Surgery - Abstract
Background: To compare the sensory change and recovery of infraorbital area associated with zygomaticomaxillary and orbital floor fractures and their recoveries and investigate the factors that affect them.Methods: We retrospectively reviewed 652 patients diagnosed with zygomaticomaxillary (n= 430) or orbital floor (n= 222) fractures in a single center between January 2016 and January 2021. Patient data, including age, sex, medical history, injury mechanism, Knight and North classification (in zygomaticomaxillary fracture cases), injury indication for surgery (in orbital floor cases), combined injury, sensory change, and recovery period, were reviewed. The chi-square test was used for statistical analysis.Results: Orbital floor fractures occurred more frequently in younger patients than zygomaticomaxillary fractures (p< 0.001). High-energy injuries were more likely to be associated with zygomaticomaxillary fractures (p< 0.001), whereas low-energy injuries were more likely to be associated with orbital floor fractures (p< 0.001). The sensory changes associated with orbital floor and zygomaticomaxillary fractures were not significantly different (p= 0.773). Sensory recovery was more rapid and better after orbital floor than after zygomaticomaxillary fractures; however, the difference was not significantly different. Additionally, the low-energy group showed a higher incidence of sensory changes than the high-energy group, but the difference was not statistically significant (p= 0.512). Permanent sensory changes were more frequent in the high-energy group, the difference was statistically significant (p= 0.043).Conclusion: The study found no significant difference in the incidence of sensory changes associated with orbital floor and zygomaticomaxillary fractures. In case of orbital floor fractures and high-energy injuries, the risk of permanent sensory impairment should be considered.
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- 2022
19. Hydrogen Isotopes Recovery Using Pd Membrane and Process Simulation
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Sang-Woo Han, Woo-Chan Jung, Hyeon-Gon Lee, Jong-Hwan Park, and Min-Ho Jang
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Membrane ,Materials science ,Isotope ,Hydrogen ,chemistry ,Inorganic chemistry ,chemistry.chemical_element ,Process simulation - Published
- 2021
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20. Intramuscular epidermal cyst in the masticator space: a case report.
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Sang Woo Han, Jiye Kim, Sug Won Kim, Minseob Eom, and Chae Eun Yang
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EPIDERMAL cyst , *DISEASE prevalence , *MAGNETIC resonance imaging - Abstract
An epidermal cyst, also known as an epidermoid cyst or epidermal inclusion cyst, is the most prevalent type of cutaneous cyst. This noncancerous lesion can appear anywhere on the body, typically presenting as an asymptomatic dermal nodule with a visible central punctum. In the case presented herein, an epidermal cyst with uncommon features was misdiagnosed as a lymphatic malformation based on preoperative magnetic resonance imaging (MRI). A 61-year-old man came to us with a swollen left cheek that had been present for 11 months. The preoperative MRI revealed a 3× 3.8× 4.6 cm lobulated cystic lesion with thin rim enhancement in the left masticator space. The initial differential diagnosis pointed toward a lymphatic malformation. We proceeded with surgical excision of the lesion via an intraoral approach, and the specimen was sent to the pathology department. The pathological diagnosis revealed a ruptured epidermal cyst, indicating that the initial diagnosis of a lymphatic malformation based on preoperative MRI was incorrect. Epidermal cysts located under the muscle with no visible central punctum are uncommon, but should be considered if a patient presents with facial swelling. Abbreviations: MRI, magnetic resonance imaging. [ABSTRACT FROM AUTHOR]
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- 2023
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21. Evolutionary origin and functional diversification of aminotransferases
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Kaan Koper, Sang-Woo Han, Delia Casas Pastor, Yasuo Yoshikuni, and Hiroshi A. Maeda
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amino acids ,Biochemistry & Molecular Biology ,enzyme evolution ,Nitrogen ,aminotransferases ,Cell Biology ,Biological Sciences ,Biochemistry ,Biological Evolution ,Medical and Health Sciences ,nitrogen metabolism ,Substrate Specificity ,Structure-Activity Relationship ,PLP-dependent enzymes ,Pyridoxal Phosphate ,Chemical Sciences ,Molecular Biology ,Transaminases - Abstract
Aminotransferases (ATs) are pyridoxal 5'-phosphate-dependent enzymes that catalyze the transamination reactions between amino acid donor and keto acid acceptor substrates. Modern AT enzymes constitute ∼2% of all classified enzymatic activities, play central roles in nitrogen metabolism, and generate multitude of primary and secondary metabolites. ATs likely diverged into four distinct AT classes before the appearance of the last universal common ancestor and further expanded to a large and diverse enzyme family. Although the AT family underwent an extensive functional specialization, many AT enzymes retained considerable substrate promiscuity and multifunctionality because of their inherent mechanistic, structural, and functional constraints. This review summarizes the evolutionary history, diverse metabolic roles, reaction mechanisms, and structure-function relationships of the AT family enzymes, with a special emphasis on their substrate promiscuity and multifunctionality. Comprehensive characterization of AT substrate specificity is still needed to reveal their true metabolic functions in interconnecting various branches of the nitrogen metabolic network in different organisms.
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- 2022
22. Investigation of phase evolution within ZnO–Bi2O3 varistors utilizing thin film prototypes
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Rongming Chu, Sang Woo Han, Chris B. DiAntonio, Kevin Ferri, Jon Paul Maria, and Elizabeth A. Paisley
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Materials science ,Dopant ,Condensed matter physics ,020502 materials ,Mechanical Engineering ,Doping ,Varistor ,Schottky diode ,02 engineering and technology ,0205 materials engineering ,Mechanics of Materials ,Phase (matter) ,General Materials Science ,Grain boundary ,Thin film ,Diffusion (business) - Abstract
Varistors are technologically important for their large energy handling capabilities and highly nonlinear electrical behavior when voltages above a characteristic switch field are applied. It is generally accepted that the prototypical ZnO–Bi2O3 varistor system forms electrostatic Schottky barriers at grain boundaries in response to residual Bi and other dopants left at grain surfaces during Bi2O3 segregation. While barrier heights can be modulated with formulation and defect chemistry, mechanisms by which dopant locations, defect compensation, and local phases determine varistor behavior are not completely understood. Bulk studies are challenging due to random grain boundary formation and difficulties studying individual boundaries. To circumvent these challenges in the ZnO–Bi2O3 varistor system, we use as-deposited and post-heat-treated thin film ZnO–Bi2O3 prototypes to simulate bulk varistor grain boundary phase formation and investigate resulting defect chemistry. Characterizing interactions between Bi2O3 films deposited on thin film and single-crystal ZnO by XRD and TEM-EDS revealed primarily Zn-out diffusion, resulting in two (Bi2O3)1−x(ZnO)x, or BZO, phases. Using these results, we present a saturated front model correlating changes in Bi2O3 thickness to phase evolution. We subsequently explore the influence of MnO doping leading to substantial changes in phase evolution for post-heat-treated (Mn:ZnO)–Bi2O3 stacks. Dopant-controlled Bi2O3 phase formations yield a 12 × difference, on average, between nonlinear coefficients for γ*- and β*-BZO.
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- 2021
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23. Efficacy analysis of virtual reality-based training for activities of daily living and functional task training in stroke patients: A single-subject study
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Lan-Ju Lee, Seong-Youl Choi, Hye-Sun Lee, and Sang-Woo Han
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General Medicine - Published
- 2023
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24. Effective removal of hazardous organic contaminant using integrated photocatalytic adsorbents: Ternary zinc oxide/zeolite-coal fly ash/reduced graphene oxide nanocomposites
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Chatchai Rodwihok, Mayulee Suwannakaew, Sang Woo Han, Ye Jee Lim, Sung Yoon Park, Sang Woon Woo, Jae Wan Choe, Duangmanee Wongratanaphisan, and Han S. Kim
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Colloid and Surface Chemistry - Published
- 2023
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25. Aromatic L-amino acid decarboxylases: mechanistic features and microbial applications
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Sang-Woo Han and Jong-Shik Shin
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Amino Acids, Aromatic ,Serotonin ,Aromatic-L-Amino-Acid Decarboxylases ,Carboxy-Lyases ,Animals ,Tyramine ,General Medicine ,Applied Microbiology and Biotechnology ,Biotechnology - Abstract
Aromatic L-amino acid decarboxylases (AADCs) catalyze the conversion of aromatic L-amino acids into aromatic monoamines that play diverse physiological and biosynthetic roles in living organisms. For example, dopamine and serotonin serve as major neurotransmitters in animals, whereas tryptamine and tyramine are essential building blocks for synthesizing a myriad of secondary metabolites in plants. In contrast to the vital biological roles of AADCs in higher organisms, microbial AADCs are found in rather a limited range of microorganisms. For example, lactic acid bacteria are known to employ AADCs to achieve intracellular pH homeostasis and engender accumulation of tyramine, causing a toxic effect in fermented foods. Owing to the crucial pharmaceutical implications of aromatic monoamines and their derivatives, synthetic applications of AADCs have attracted growing attention. Besides, recent studies have uncovered that AADCs of human gut microbes influence host physiology and are involved in drug availability of Parkinson's disease medication. These findings bring the bacterial AADCs into a new arena of extensive research for biomedical applications. Here, we review catalytic features of AADCs and present microbial applications and challenges for biotechnological exploitation of AADCs. KEY POINTS: • Aromatic monoamines and their derivatives are increasingly important in the drug industry. • Aromatic L-amino acid decarboxylases are the only enzyme for synthesizing aromatic monoamines. • Microbial applications of aromatic L-amino acid decarboxylases have drawn growing attention.
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- 2022
26. Experimental Demonstration of Charge- Balanced GaN Super-Heterojunction Schottky Barrier Diode Capable of 2.8 kV Switching
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Robert M. Lavelle, David W. Snyder, Jianan Song, Rongming Chu, Sang-Woo Han, Thomas N. Jackson, Joan M. Redwing, Sang Ha Yoo, and Ziguang Ma
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010302 applied physics ,Materials science ,business.industry ,Schottky barrier ,Doping ,Schottky diode ,Gallium nitride ,Heterojunction ,01 natural sciences ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,chemistry ,Etching ,0103 physical sciences ,Breakdown voltage ,Optoelectronics ,Electrical and Electronic Engineering ,business ,Diode - Abstract
This letter reports an experimental demonstration of charge-balanced GaN super-heterojunction Schottky barrier diodes (SHJ-SBDs). Charge balance between the n-type delta-doping and the p-type doping was achieved by adjusting the thickness of the pGaN. This device structure enabled scaling of breakdown voltage to over 3 kV, and dynamic switching up to 2.8 kV without using any field-plate.
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- 2020
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27. A Layout Design System of Hard Defense and Soft Defense Methods for Coastal Erosion Mitigation
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Jung Lyul Lee, Uk Han Lee, and Sang Woo Han
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Page layout ,Environmental science ,computer.software_genre ,Civil engineering ,computer ,Coastal erosion - Published
- 2020
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28. Multitoken-Based Power Management for NAND Flash Storage Devices
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Young Min Park, Hyuk-Jun Lee, Taehee You, Sang-Woo Han, and Eui-Young Chung
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Power management ,business.industry ,Computer science ,Nand flash memory ,NAND gate ,Deadlock ,Security token ,Computer Graphics and Computer-Aided Design ,Power budget ,Embedded system ,Key (cryptography) ,Overhead (computing) ,Electrical and Electronic Engineering ,business ,Throughput (business) ,Software - Abstract
NAND flash-based storage devices (NFSDs) have been widely employed in various systems, including cloud servers as well as mobile devices. The core component of NFSDs is NAND flash memory (NFM) which has several advantages over the conventional hard disk drives (HDDs). An NFSD typically adopts a bunch of NFMs which are operated in parallel for maximizing the I/O throughput. However, optimizing for performance may not be desirable from the power budget (PB) perspective. In other words, concurrent operations of NFMs often drain inordinate current, which leads to the violation of the PB allocated for a storage device. In this article, we propose a novel power management scheme which maximizes concurrent operations of NFMs under the given power constraint. The proposed method quantizes the given power constraint of an NFSD. A quantum also called token is the basic unit of power management. The proposed power management scheme allocates tokens to NFMs and only the NFMs having enough tokens can perform their operations. We call this method multitoken-based power management (MTPM). The critical issue of MTPM is a deadlock which is resolved with the key allocation scheme. Furthermore, we enhance MTPM to improve performance. The extended method called keyless MTPM (KMTPM) improves the overall performance by relaxing the key acquisition requirement and allowing subatomic operations. In the experimental results, we confirm that the proposed methods always meet the given power constraint. The proposed KMTPM improves throughput by 22.85% compared to state of the art technique. In addition, KMTPM only incurs 3.8% of performance overhead and 0.015% of area overhead.
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- 2020
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29. A study on the influence of temperature and relative humidity and the concentrations calibration for H2S and NH3 sensors
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Chun Sang Lee, Sun Hyang Lim, Kyoung Chan Kim, Hung Soo Joo, Sang Woo Han, Hyeon Seop Jang, and Jin Seok Han
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Materials science ,Calibration (statistics) ,Analytical chemistry ,Relative humidity - Published
- 2020
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30. Structure‐based design of peptides that trigger Streptococcus pneumoniae cell death
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Chenglong Jin, Do-Hee Kim, Sung Jean Park, Sung-Min Kang, Sang Woo Han, and Bong-Jin Lee
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0301 basic medicine ,Protein Conformation, alpha-Helical ,Cell Membrane Permeability ,Protein Data Bank (RCSB PDB) ,Gene Expression ,Peptide binding ,medicine.disease_cause ,Crystallography, X-Ray ,Protein Engineering ,Biochemistry ,Cell membrane ,0302 clinical medicine ,Protein structure ,Drug Discovery ,Transcriptional regulation ,Cloning, Molecular ,Chemistry ,Toxin-Antitoxin Systems ,Toxin-antitoxin system ,Recombinant Proteins ,Molecular Docking Simulation ,medicine.anatomical_structure ,Streptococcus pneumoniae ,antibacterial strategy ,030220 oncology & carcinogenesis ,Original Article ,Antitoxin ,ribonuclease ,Allosteric Site ,Protein Binding ,Bacterial Toxins ,Genetic Vectors ,Microbial Sensitivity Tests ,03 medical and health sciences ,Structure-Activity Relationship ,Allosteric Regulation ,medicine ,Escherichia coli ,Protein Interaction Domains and Motifs ,Molecular Biology ,toxin–antitoxin system ,Cell Biology ,Original Articles ,030104 developmental biology ,DNA‐binding ,Drug Design ,Protein Conformation, beta-Strand ,Antitoxins ,Antimicrobial Cationic Peptides - Abstract
Toxin–antitoxin (TA) systems regulate key cellular functions in bacteria. Here, we report a unique structure of the Streptococcus pneumoniae HigBA system and a novel antimicrobial agent that activates HigB toxin, which results in mRNA degradation as an antibacterial strategy. In this study, protein structure‐based peptides were designed and successfully penetrated the S. pneumoniae cell membrane and exerted bactericidal activity. This result represents the time during which inhibitors triggered S. pneumoniae cell death via the TA system. This discovery is a remarkable milestone in the treatment of antibiotic‐resistant S. pneumoniae, and the mechanism of bactericidal activity is completely different from those of current antibiotics. Furthermore, we found that the HigBA complex shows a crossed‐scissor interface with two intermolecular β‐sheets at both the N and C termini of the HigA antitoxin. Our biochemical and structural studies provided valuable information regarding the transcriptional regulation mechanisms associated with the structural variability of HigAs. Our in vivo study also revealed the potential catalytic residues of HigB and their functional relationships. An inhibition study with peptides additionally proved that peptide binding may allosterically inhibit HigB activity. Overall, our results provide insights into the molecular basis of HigBA TA systems in S. pneumoniae, which can be applied for the development of new antibacterial strategies. Databases Structural data are available in the PDB database under the accession number 6AF4., HigBA complex shows a crossed‐scissor interface with two intermolecular β‐sheets at both the N and C termini of the HigA antitoxin. The crossed‐scissor constitutes a new type of interface between HigB and HigA. We report a unique structure of the Streptococcus pneumoniae HigBA system and a novel antimicrobial agent that activates HigB toxin, which results in mRNA degradation as an antibacterial strategy.
- Published
- 2020
31. Design of GaN/AlGaN/GaN Super-Heterojunction Schottky Diode
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Rongming Chu, Jianan Song, and Sang-Woo Han
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010302 applied physics ,Materials science ,business.industry ,Doping ,Schottky diode ,Heterojunction ,Gallium nitride ,Electron ,Epitaxy ,01 natural sciences ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,chemistry ,0103 physical sciences ,Optoelectronics ,Breakdown voltage ,Electrical and Electronic Engineering ,Fermi gas ,business - Abstract
We present a systematic study on the design of a novel GaN/AlGaN/GaN super-heterojunction Schottky diode. Through physics-based TCAD simulation, we discuss three important design aspects: 1) how to design a GaN/AlGaN/GaN structure to form a high-density 2-D electron gas and to scale it to multiple vertically stacked channels with less risk in reaching the critical thickness limited by the strain in epitaxy; 2) how to reach charge balance and how sensitive is the breakdown voltage with respect to the doping imbalance; and 3) how to ensure that the processes of depleting and accumulating electrons and holes in the structure are fast enough for practical power switching applications.
- Published
- 2020
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32. Microbiome engineering for sustainable agriculture: using synthetic biology to enhance nitrogen metabolism in plant-associated microbes
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Sang-Woo Han and Yasuo Yoshikuni
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Microbiology (medical) ,Crops, Agricultural ,Infectious Diseases ,Nitrogen ,Microbiota ,Agriculture ,Synthetic Biology ,Prospective Studies ,Microbiology - Abstract
Plants benefit from symbiotic relationships with their microbiomes. Modifying these microbiomes to further promote plant growth and improve stress tolerance in crops is a promising strategy. However, such efforts have had limited success, perhaps because the original microbiomes quickly re-establish. Since the complex biological networks involved are little understood, progress through conventional means is time-consuming. Synthetic biology, with its practical successes in multiple industries, could speed up this research considerably. Some fascinating candidates for production by synthetic microbiomes are organic nitrogen metabolites and related pyridoxal-5'-phosphate-dependent enzymes, which have pivotal roles in microbe-microbe and plant-microbe interactions. This review summarizes recent studies of these metabolites and enzymes and discusses prospective synthetic biology platforms for sustainable agriculture.
- Published
- 2022
33. Coupled simulation of thermal-metallurgical-mechanical behavior in laser keyhole welding of AH36 steel
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Sang-Woo Han, Suck-Joo Na, Linjie Zhang, and Won-Ik Cho
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Materials science ,Vacuum ,business.industry ,CFD-FEM combined simulation ,Mechanical Engineering ,Metallurgy ,Welding ,Numerical simulation ,Computational fluid dynamics ,Thermal conduction ,Phase transformation ,Finite element method ,Laser keyhole welding ,law.invention ,Mechanics of Materials ,law ,Residual stress ,Vickers hardness test ,TA401-492 ,Stochastic ray tracing ,General Materials Science ,business ,Material properties ,Keyhole ,Materials of engineering and construction. Mechanics of materials - Abstract
A computational fluid dynamics (CFD) simulation of the molten pool in laser keyhole welding was utilized to acquire temperature data for further metallurgical and mechanical calculations. For the CFD simulation, the governing equations were solved, and the scattering and absorption of the laser beam in the plume were modeled at both the standard atmospheric condition (101,325 Pa) and a vacuum condition (3,000 Pa). A stochastic ray-tracing algorithm was adopted to effectively implement the transmission and scattering of laser bundles of rays. The temperature data from the CFD simulation were then imported to a finite element method (FEM)-based heat conduction analysis to simulate the thermal-metallurgical-mechanical behavior during the cooling phase of the weldment. The strain, residual stress, and distortion were calculated using an elastoplastic model based on the phase transformation-dependent material properties. An element deactivation scheme was used to take care of the zero-strength condition of the elements in the molten pool and keyhole region. The Vickers hardness and the residual stress were measured to verify the simulation model, and the experimental and simulation results had a similar tendency.
- Published
- 2021
34. Source Apportionment of PM2.5 in Daejeon Metropolitan Region during January and May to June 2021 in Korea Using a Hybrid Receptor Model
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Sang-Woo Han, Hung-Soo Joo, Hui-Jun Song, Su-Bin Lee, and Jin-Seok Han
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Atmospheric Science ,Environmental Science (miscellaneous) ,PM2.5 ,positive matrix factorization ,emission source ,modified concentration weight trajectory ,hybrid receptor model - Abstract
We tried to estimate anthropogenic emission sources, including the contributions of neighboring regions, that affect the fine particle concentration (PM2.5) in Daejeon using positive matrix factorization (PMF), concentration weight trajectory (CWT), and modified concentration weight trajectory (MCWT) models in a manner that might overcome the limitations of widely applied hybrid receptor models. Fractions of ion, carbonaceous compound and elements in PM2.5 were 58%, 17%, and 3.6% during January and 49%, 17%, and 14.9% during May to June, respectively. The fraction of ions was higher during winter season, while the fraction of elements was higher during the other season. From the PMF model, seven factors were determined, including dust/soil, sea salt, secondary nitrate/chloride, secondary sulfate, industry, coal combustion, and vehicle sources. Secondary sulfate showed the highest contribution followed by secondary nitrate/chloride and vehicle sources. The MCWT model significantly improved the performance of regional contributions of the CWT model, which had shown a high contribution from the Yellow Sea where there are no emission sources. According to the MCWT results, regional contributions to PM2.5 in the Daejeon metropolitan region were highest from eastern and southern China, followed by Russia, northeastern China, and Manchuria. We conclude that the MCWT model is more useful than the CWT model to estimate the regional influence of the PM2.5 concentrations. This approach can be used as a reference tool for studies to further improve on the limitations of hybrid receptor models.
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- 2022
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35. High-${Q}$ GaN Varactors for mm-Wave Applications: A Physics-Based Simulation Study
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Jianan Song, Sang-Woo Han, and Rongming Chu
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010302 applied physics ,Materials science ,Equivalent series resistance ,business.industry ,Schottky diode ,Gallium nitride ,Heterojunction ,01 natural sciences ,Capacitance ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,chemistry ,Q factor ,0103 physical sciences ,Optoelectronics ,Electrical and Electronic Engineering ,business ,Varicap ,Voltage - Abstract
Varactors, with its capacitance tunable by the applied voltage, are enabling components for reconfigurable RF systems. The performance of a varactor could be characterized by its ${Q}$ -factor and the capacitance tuning ratio. In this article, we seek to develop a new varactor with very high ${Q}$ -factor and large tuning ratio, enabling reconfigurable RF systems with large bandwidth. There were two main innovations in varactor diode design. One was the use of the fin structure, in which the electrodes were placed on two opposite sidewalls of the mesa structure instead of one on top and the other at the bottom or next to the mesa. This design eliminates the extrinsic series resistance in the conventional varactor diode designs, leading to substantially increased ${Q}$ -factor. The other was the use of a multichannel structure. The structure was composed of multiple AlGaN/GaN heterojunctions with high-mobility 2-D electron gas. The high-mobility electron gas helped lowering the intrinsic series resistance, thereby further improving the ${Q}$ -factor.
- Published
- 2019
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36. Ultrafast Electron Microscopy Visualizes Acoustic Vibrations of Plasmonic Nanorods at the Interfaces
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Oh-Hoon Kwon, Sang Woo Han, Hayoon Jung, and Ye-Jin Kim
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Materials science ,business.industry ,Detector ,Physics::Optics ,Electron ,Photoexcitation ,Picosecond ,Physics::Atomic and Molecular Clusters ,Cathode ray ,Optoelectronics ,General Materials Science ,Nanorod ,Physics::Chemical Physics ,business ,Ultrashort pulse ,Plasmon - Abstract
Summary By integrating a direct electron detector with ultrafast electron microscopy and controlling the quality of the pulsed electron beam, unprecedented spatiotemporal resolution in real-space imaging was achieved under practical pump-probe measurements. As a proof-of-principle demonstration of this new technique, the acoustic vibrations of plasmonic nanorods that were one nanometer amplitude or less and picosecond period upon femtosecond-pulsed photoexcitation were studied at the frictional interfaces. The subnanometer sensitivity in visualizing individual mechanical movement captures new details about acoustic vibrations such as the initial launching of the vibrations and the suppression and release of the vibration at the interfaces.
- Published
- 2019
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37. A Locality-Aware Compression Scheme for Highly Reliable Embedded Systems
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Eui-Young Chung, Jeongbin Kim, Sang-Woo Han, and Juhyung Hong
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Dynamic random-access memory ,Hardware_MEMORYSTRUCTURES ,Computer science ,business.industry ,Locality ,FLAGS register ,02 engineering and technology ,DIMM ,Computer Graphics and Computer-Aided Design ,Memory controller ,020202 computer hardware & architecture ,law.invention ,Memory module ,law ,Embedded system ,0202 electrical engineering, electronic engineering, information engineering ,Electrical and Electronic Engineering ,business ,Software ,Access time ,Dram ,Data compression - Abstract
Dynamic random access memory (DRAM) reliability has become one of the critical issues in embedded systems, as DRAM process technology advances with the increase in bit error probability. Unfortunately, redundant error-correction code (ECC) chips cannot be applied to embedded systems since cores and DRAMs are tightly coupled without a dual in-line memory module (DIMM) slot to account for the form factor, cost, and limited pin count. Therefore, ECC parities are typically placed in the same physical array where the user and system data reside. This coexistence eventually deteriorates data locality, which could be the critical factor in DRAM performance degradation. To address this issue, we propose an ECC scheme called locality-aware compression (LoComp) which integrates a compression algorithm, DRAM data layout, and memory controller especially optimized for embedded systems. We focus on the locality of the dataset and its corresponding metadata, as well as spatial data locality in the design of DRAM data layout, which reduces the number of row activations. The major feature in a compression algorithm is adjusting the misalignment of data streams caused by the data packing in many embedded systems. Moreover, we specialize the memory controller to reduce DRAM access for ECC parities and compression flags. The core technologies for the memory controller are the adoption of a set of small caches for metadata and the support of partial write operation without changing the DRAM interface. LoComp+, an enhanced version of LoComp, further reduces DRAM access for metadata by placing the metadata close to the corresponding data. In the experiment, previous works increase the DRAM access time from 68% to over twice the value compared to ECC DIMM. Whereas, LoComp and LoComp+ show reduced performance degradation by 33% and 48%, respectively. In other words, LoComp and LoComp+ substantially improved performance from between 13% and 33% compared to previous embedded ECC schemes.
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- 2019
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38. 3D Structural Determination of Core-shell Nanoparticles
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Hyesung Jo, Dae Han Wi, Taegu Lee, Chaehwa Jeong, Juhyeok Lee, Hionsuck Baik, Alexander J Pattison, Wolfgang Theis, Colin Ophus, Peter Ercius, Seunghwa Ryu, Sang Woo Han, and Yongsoo Yang
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Instrumentation - Published
- 2022
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39. Pd–Ag Bimetallic Catalysts with Core–Shell Engineering for Efficient Hydrogen Production from Formic Acid Decomposition
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Bon Seung Goo and Sang Woo Han
- Abstract
Fine control of the ligand and strain effects of secondary elements on the catalytic function of primary elements is critical for developing high-performance bimetallic catalysts. In this paper, we describe a method for making Pd–Ag bimetallic core–shell nanocatalysts with synergistic ligand and Ag strain effects. (PdAg alloy core)@(ultrathin Pd shell) nanocrystals with regulated core compositions and shell thicknesses, as well as a well-defined octahedral shape, could be achieved through precision core–shell engineering. The produced octahedral PdAg@Pd core–shell nanocrystals showed excellent catalytic activity in the creation of hydrogen from the breakdown of formic acid. The highest catalytic activity was attained using PdAg@Pd nanocrystals made up of PdAg alloy cores with an average Pd/Ag atomic ratio of 3.5:1 and a 1.1 atomic layer of Pd shells, which set a new record for catalytic activity.
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- 2022
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40. One-Pot Production of Pt–Ni Alloy Nanodendrites on Ceria Nanosheet Supporting Materials with High Electrocatalytic Performance Toward Methanol Oxidation and Oxygen Reduction
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Yongmin Kwon, Eun Jin Lee, and Sang Woo Han
- Abstract
To create better fuel cell catalysts, effective approaches to generate hybrid materials comprised of intimately connected metal nanocatalysts and nonstoichiometric metal oxides with finely regulated topologies are very desirable. The production of closely connected metal (PtNi alloy)–metal oxide (CeOx, ceria) hybrid nanostructures is reported here using a simple one-pot synthesis technique. Heating a mixed metal (Pt, Ni, and Ce) precursor solution in oleylamine/oleic acid resulted in well-defined PtNi/ceria hybrids, in which PtNi alloy nanodendrites with multiple branches are evenly spread over small-grained ceria nanosheets. The ceria nanosheets in the hybrids have a large number of oxygen vacancies and a high conductivity due to their small grain size. In comparison to carbon-supported counterparts and a commercial catalyst, the prepared PtNi/ceria hybrids exhibited outstanding electrocatalytic activity and stability for both methanol oxidation and oxygen reduction reactions, which can be attributed to the synergistic effects of the Ni component and ceria on the enhancement of the electrocatalytic function. The current technique will be used to create the best multicomponent catalysts for specific processes.
- Published
- 2022
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41. Nanogap-tailored Au nanoparticles fabricated by pulsed laser ablation for surface-enhanced Raman scattering
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Hyeyeon Lee, Yonghyeon Kim, Myong Yong Choi, Sang Woo Han, Seung Jun Lee, Yiseul Yu, Talshyn Begildayeva, Jayaraman Theerthagiri, and Young Wook Lee
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Materials science ,Biomedical Engineering ,Biophysics ,Nanoparticle ,Metal Nanoparticles ,General Medicine ,Biosensing Techniques ,Spectrum Analysis, Raman ,Pulsed laser ablation ,Solvent ,symbols.namesake ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Colloidal gold ,Electrochemistry ,symbols ,Graphitic carbon ,Molecule ,Gold ,Laser Therapy ,Acetonitrile ,Raman scattering ,Biotechnology - Abstract
Herein, gold nanoparticles (Au NPs) were synthesized by pulsed laser ablation (PLA) in a mixed-phase solvent of acetonitrile and water. The size of Au NPs and the number of graphitic carbon (GC) layers were controlled by varying the ratio of the solvent mixture. The surface-enhanced Raman scattering (SERS) of the Au NPs was investigated using 10−3 M 4-aminobenzenethiol and 10−4 M 4-nitrobenzenethiol as probe molecules. The SERS activity strongly depended on the nanogaps between particles owing to the formation of hot spots. In the present work, the nanogaps were controlled by changing the amount of GC layers. No GC layers were produced in water, resulting low SERS intensity. In contrast, Au NPs prepared in 30 vol% of acetonitrile showed significant SERS enhancement, which was attributed to the optimal size of the GC-coated NPs and a reasonable gap between them. The obtained results revealed that Au NPs produced by PLA in liquid could be applied in SERS-based microsensors.
- Published
- 2021
42. Toxin-Activating Stapled Peptides Discovered by Structural Analysis Were Identified as New Therapeutic Candidates That Trigger Antibacterial Activity against Mycobacterium tuberculosis in the Mycobacterium smegmatis Model
- Author
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Heejo Moon, Byeong Wook Kim, Do-Hee Kim, Sung-Min Kang, Bong-Jin Lee, Sang Woo Han, and Byeong Moon Kim
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Microbiology (medical) ,Tuberculosis ,toxin-antitoxin system ,medicine.drug_class ,Antibiotics ,Peptide ,Microbiology ,Article ,Mycobacterium tuberculosis ,03 medical and health sciences ,Virology ,vapBC ,medicine ,lcsh:QH301-705.5 ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,biology ,Mycobacterium smegmatis ,030302 biochemistry & molecular biology ,structure-based drug discovery ,stapled peptide ,medicine.disease ,biology.organism_classification ,Toxin-antitoxin system ,Antimicrobial ,lcsh:Biology (General) ,chemistry ,antimicrobial candidate - Abstract
The structure-function relationships of toxin-antitoxin (TA) systems from Mycobacterium tuberculosis have prompted the development of novel and effective antimicrobial agents that selectively target this organism. The artificial activation of toxins by peptide inhibitors can lead to the growth arrest and eventual death of bacterial cells. Optimizing candidate peptides by hydrocarbon α-helix stapling based on structural information from the VapBC TA system and in vitro systematic validation led to V26-SP-8, a VapC26 activator of M. tuberculosis. This compound exhibited highly enhanced activity and cell permeability owing to the stabilizing helical propensity of the peptide. These characteristics will increase its efficacy against multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis. Similar approaches utilizing structural and biochemical information for new antibiotic targets opens a new era for developing TB therapies.
- Published
- 2021
43. Comparison of the Qualitative and the Quantitative Risk Assessment of Hazardous Substances Requiring Management under the Occupational Safety and Health Act in South Korea
- Author
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Sang Hoon Byeon, Sang Woo Han, Hyung Il Moon, and Saemi Shin
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Health, Toxicology and Mutagenesis ,lcsh:Medicine ,qualitative risk assessment ,Risk Assessment ,Occupational safety and health ,Hazardous Substances ,Article ,03 medical and health sciences ,0302 clinical medicine ,Exposure level ,Hazardous waste ,Environmental health ,Occupational Exposure ,Republic of Korea ,Medicine ,030212 general & internal medicine ,Risk management ,Occupational Health ,occupational safety and health act ,business.industry ,Control banding ,lcsh:R ,Public Health, Environmental and Occupational Health ,quantitative risk assessment ,030210 environmental & occupational health ,Hazard ,Chemical hazard ,hazardous substances requiring management ,control banding ,business ,Risk assessment - Abstract
The risk assessment of hazardous substances has become increasingly important for the efficient prevention and management of various diseases or accidents caused by increased amounts of hazardous substances in the workplace. In this study, risk assessment was conducted for 36 kinds of hazardous substances requiring management by using qualitative and quantitative risk assessments. Qualitative risk assessment was performed by multiplying the exposure level class by the hazard class according to the Korea Occupational Safety and Health Agency’s (KOSHA) Chemical Hazard Risk Management (CHARM). The quantitative risk assessment was followed by a four-step risk assessment system presented in the Guidelines for Hazard Risk Assessment of Chemicals (KOSHA GUIDE W-6-2016). In the quantitative assessments, we presented a new method of classifying risk levels into four steps, much like qualitative assessments. In this study, the quantitative risk assessment was considered difficult to predict through qualitative risk assessment. Therefore, it is necessary to perform a quantitative risk assessment after a qualitative risk assessment for a higher level of risk assessment.
- Published
- 2021
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44. Rapid Diagnosis of Coronavirus by RNA-Directed RNA Transcription Using an Engineered RNA-based Platform
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Sun-Wook Jeong, Jong Bum Lee, Yong Jun Choi, Sang Woo Han, Geonhu Lee, and Ohsung Ko
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Letter ,RNA virus ,Transcription, Genetic ,viruses ,Bioengineering ,02 engineering and technology ,medicine.disease_cause ,RNA-dependent RNA polymerase (RdRP) ,chemistry.chemical_compound ,Transcription (biology) ,RNA polymerase ,medicine ,CRISPR ,Humans ,Nanotechnology ,General Materials Science ,Gene ,Pandemics ,Coronavirus ,biology ,SARS-CoV-2 ,Mechanical Engineering ,RNA ,COVID-19 ,General Chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,RNA-Dependent RNA Polymerase ,Condensed Matter Physics ,Virology ,Real-time polymerase chain reaction ,chemistry ,COVID-19 Nucleic Acid Testing ,2019-nCoV ,RNA, Viral ,0210 nano-technology ,Genetic Engineering - Abstract
A coronavirus disease (COVID-19) outbreak associated with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading widely through person-to-person transmission. Various detection approaches have been developed involving quantitative polymerase chain reaction (qPCR) methods, CRISPR-based systems, and direct targeting of specific coronavirus proteins. However, there have only been a few reports on the detection of RNA-dependent RNA polymerase (RdRP), the primer-independent RNA-replicable protein produced by the RNA genes of coronavirus. Here, we introduce a novel diagnostic methodology for COVID-19 using the RNA-directed and de novo RNA replicable function of RdRP. We devised an RNA platform for RdRP-induced transcription (RPRIT) that includes an RNA template that can be directly transcribed by RdRP. By utilizing RPRIT, the presence of RdRP can be readily confirmed within 30 min using isothermal incubation without PCR. This RdRP detection method can provide a new route for rapid diagnosis of RNA virus-infected patients.
- Published
- 2021
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45. Core-Shell Bimetallic Nanoparticle Trimers for Efficient Light-to-Chemical Energy Conversion
- Author
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Johan Grand, Won-Seok Lee, Sang Woo Han, Baptiste Auguié, Emiliano Cortés, Heeyeon Hwang, Seunghoon Lee, Dmitri Scherbarchov, Younghyun Wy, and Dae Han Wi
- Subjects
Materials science ,Energy Engineering and Power Technology ,Nanoparticle ,FOS: Physical sciences ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,7. Clean energy ,Chemical reaction ,Catalysis ,Physics - Chemical Physics ,Materials Chemistry ,Reactivity (chemistry) ,Chemical Physics (physics.chem-ph) ,Aqueous solution ,Renewable Energy, Sustainability and the Environment ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Chemical energy ,Fuel Technology ,Chemical engineering ,Chemistry (miscellaneous) ,Photocatalysis ,0210 nano-technology ,Physics - Optics ,Visible spectrum ,Optics (physics.optics) - Abstract
Incorporation of catalytically active materials into plasmonic metal nanostructures can efficiently merge the reactivity and energy harvesting abilities of both types of materials for visible light photocatalysis. Here we explore the influence of electromagnetic hotspots in the ability of plasmonic core-shell colloidal structures to induce chemical transformations. For this study, we developed a synthetic strategy for the fabrication of Au nanoparticle (NP) trimers in aqueous solution through fine controlled galvanic replacement between Ag nanoprisms and Au precursors. Core-shell Au@M NP trimers with catalytically active metals (M = Pd, Pt) were subsequently synthesized using Au NP trimers as templates. Our experimental and computational results highlight the synergy of geometry and composition in plasmonic catalysts for plasmon-driven chemical reactions.
- Published
- 2021
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46. Evolutionary origin and functional diversification of aminotransferases.
- Author
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Koper, Kaan, Sang-Woo Han, Pastor, Delia Casas, Yasuo Yoshikuni, and Maeda, Hiroshi A.
- Subjects
- *
AMINOTRANSFERASES , *METABOLITES , *KETONIC acids , *AMINO acids , *FAMILY relations - Abstract
Aminotransferases (ATs) are pyridoxal 50-phosphate-dependent enzymes that catalyze the transamination reactions between amino acid donor and keto acid acceptor substrates. Modern AT enzymes constitute 2% of all classified enzymatic activities, play central roles in nitrogen metabolism, and generate multitude of primary and secondary metabolites. ATs likely diverged into four distinct AT classes before the appearance of the last universal common ancestor and further expanded to a large and diverse enzyme family. Although the AT family underwent an extensive functional specialization, many AT enzymes retained considerable substrate promiscuity and multifunctionality because of their inherent mechanistic, structural, and functional constraints. This review summarizes the evolutionary history, diverse metabolic roles, reaction mechanisms, and structure-function relationships of the AT family enzymes, with a special emphasis on their substrate promiscuity and multifunctionality. Comprehensive characterization of AT substrate specificity is still needed to reveal their true metabolic functions in interconnecting various branches of the nitrogen metabolic network in different organisms. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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47. Biochemical characterization and synthetic application of aromatic L-amino acid decarboxylase from Bacillus atrophaeus
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Jun Sung Kim, Jong Shik Shin, Youngho Jang, Yeri Choi, and Sang-Woo Han
- Subjects
Serotonin ,Stereochemistry ,Decarboxylation ,Bacillus ,Applied Microbiology and Biotechnology ,5-Hydroxytryptophan ,03 medical and health sciences ,chemistry.chemical_compound ,Biogenic amine ,Aromatic amino acids ,Enzyme kinetics ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Aromatic L-amino acid decarboxylase ,biology ,030306 microbiology ,Chemistry ,Tryptophan ,Substrate (chemistry) ,General Medicine ,biology.organism_classification ,Bacillus atrophaeus ,Aromatic-L-Amino-Acid Decarboxylases ,Yield (chemistry) ,Biotechnology - Abstract
Aromatic L-amino acid decarboxylases (AADCs) are ubiquitously found in higher organisms owing to their physiological role in the synthesis of neurotransmitters and alkaloids. However, bacterial AADC has not attracted much attention because of its rather limited availability and narrow substrate range. Here, we examined the biochemical properties of AADC from Bacillus atrophaeus (AADC-BA) and assessed the synthetic feasibility of the enzyme for the preparation of monoamine neurotransmitters. AADC-BA was expressed in Escherichia coli BL21(DE3) and the purified enzyme showed a specific activity of 2.6 ± 0.4 U/mg for 10 mM L-phenylalanine (L-Phe) at 37 °C. AADC-BA showed optimal pH and temperature ranges at 7-8 and 37-45 °C, respectively. The KM and kcat values for L-Phe were 7.2 mM and 7.4 s-1, respectively, at pH 7.0 and 37 °C. Comparison of the kinetic constants at different temperatures revealed that the temperature dependency of the enzyme was mainly determined by catalytic turnover rather than substrate binding. AADC-BA showed a broad substrate scope for various aromatic amino acids, including L-Phe, L-tryptophan (610% relative to L-Phe), L-tyrosine (12%), 3,4-dihydroxyphenyl-L-alanine (24%), 5-hydroxy-L-tryptophan (L-HTP, 71%), 4-chloro-L-phenylalanine (520%), and 4-nitro-L-phenylalanine (450%). Homology modeling and docking simulations were carried out and were consistent with the observed substrate specificity. To demonstrate the synthetic potential of AADC-BA, we carried out the production of serotonin by decarboxylation of L-HTP. The reaction yield of serotonin reached 98% after 1 h at the reaction conditions of 50 mM L-HTP and 4 U/mL AADC-BA. Moreover, we carried out preparative-scale decarboxylation of L-Phe (100 mM in 40-mL reaction mixture) and isolated the resulting 2-phenylethylamine (51% recovery yield). We expect that the broad substrate specificity of AADC-BA can be exploited to produce various aromatic biogenic amines. KEY POINTS: • AADC-BA showed broad substrate specificity for various aromatic amino acids. • The substrate specificity was elucidated by in silico structural modeling. • The synthetic potential of AADC-BA was demonstrated for the production of biogenic amines.
- Published
- 2020
48. GaN based negative capacitance heterojunction field-effect transistors with <30 mV/dec subthreshold slope for steep switching operation
- Author
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Su-Keun Eom, Kwang-Suk Seo, Myoung-Jin Kang, Sang-Woo Han, Hyun-Seop Kim, and Ho-Young Cha
- Subjects
010302 applied physics ,Materials science ,Subthreshold conduction ,business.industry ,Transistor ,General Physics and Astronomy ,Gallium nitride ,Heterojunction ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Subthreshold slope ,lcsh:QC1-999 ,law.invention ,chemistry.chemical_compound ,Atomic layer deposition ,chemistry ,law ,0103 physical sciences ,Optoelectronics ,Field-effect transistor ,0210 nano-technology ,business ,lcsh:Physics ,Negative impedance converter - Abstract
We demonstrated gallium nitride (GaN) based negative capacitance field-effect transistors (NCFETs) that achieve steep switching operation. Ferroelectric phase characteristics were successfully demonstrated using a 10 nm thick undoped-HfO2 ferroelectric thin film fabricated through atomic layer deposition. The HfO2 metal-ferroelectric-metal (MFM) capacitor was connected in series with the gate electrode of a recessed normally-off AlGaN/GaN MOS-HFET. The forward/reverse subthreshold slopes were dramatically reduced from 104/105 to 22/23 mV/dec.
- Published
- 2020
49. Electrical Properties of Horizontal Array Capacitor Using Composite Organic Dielectric Layer of Impregnated Glass-Fiber Epoxy
- Author
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Ho-Young Cha, Sang-Min Lee, Su-Jeong Suh, Hwa-Sun Park, Sang Gweon Kim, Joon-kyun Lee, Sang-Woo Han, Yong-Soo Oh, Young-Il Na, and Jung-Rag Yoon
- Subjects
Materials science ,Polymers and Plastics ,Article Subject ,020502 materials ,General Chemical Engineering ,020208 electrical & electronic engineering ,Organic Chemistry ,02 engineering and technology ,Dielectric ,Capacitance ,law.invention ,Capacitor ,TP1080-1185 ,0205 materials engineering ,law ,Lamination ,Hardware_INTEGRATEDCIRCUITS ,0202 electrical engineering, electronic engineering, information engineering ,Array data structure ,Polymers and polymer manufacture ,Resistor ,Composite material ,Layer (electronics) ,Electrical impedance - Abstract
We introduce a horizontal array capacitor with nine capacitances in a single body using an organic dielectric layer impregnated with glass fiber as a prepreg sheet. An organic solid horizontal array capacitor with a dielectric of prepreg materials of the epoxy type can implement the nine capacitances in a single body via a unique simple lamination and cutting process. We then investigate the basic electrical properties of a horizontal array capacitor. The organic solid array capacitors with five electrodes and four dielectrics are Cu/PPG layer/Cu/PPG layer/Cu/PPG layer/Cu/PPG layer/Cu with a horizontal array structure. The size of a completed array capacitor is 2.85 × 2.85 mm. The height of the fabricated array capacitor in the vertical direction is 0.5 mm, with nine capacitances possessing a series-type structure. The average capacitance value of C1, C2, C3, and C4 is 1.98 nF, and each tolerance has a value within 1% based on the average value. The temperature change rate in the capacitance maintains a nearly linear characteristic, but the rate of change tends to increase finely from 120°C or more. The capacitance values of C5, C6, and C7 with the parallel circuit were measured according to the voltage. Impedance and ESR (equivalent series resistor) of C1 were measured according to frequency and temperature.
- Published
- 2020
- Full Text
- View/download PDF
50. Investigation of Defect Distributions in SiO2/AlGaN/GaN High-Electron-Mobility Transistors by Using Capacitance-Voltage Measurement with Resonant Optical Excitation
- Author
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Ho-Young Cha, Tae-Soo Kim, Yong-Keun Park, Seung-Young Lim, Sang-Woo Han, Gunwoo Jung, and Jung-Hoon Song
- Subjects
010302 applied physics ,Materials science ,business.industry ,Schottky barrier ,Transistor ,General Physics and Astronomy ,02 engineering and technology ,High-electron-mobility transistor ,021001 nanoscience & nanotechnology ,01 natural sciences ,law.invention ,Threshold voltage ,Capacitance voltage ,law ,0103 physical sciences ,Optoelectronics ,0210 nano-technology ,business ,High electron ,Excitation ,Voltage - Abstract
We investigated the distributions and the energy levels of defects in SiO2/AlGaN/GaN highelectron-mobility transistors (HEMTs) by using frequency-dependent (F-D) capacitance-voltage (C-V) measurements with resonant optical excitation. A Schottky barrier (SB) and a metal-oxidesemiconductor (MOS) HEMT were prepared to compare the effects of defects in their respective layers. We also investigated the effects of those layers on the threshold voltage (V th ). A drastic voltage shift in the C-V curve at higher frequencies was caused by the large number of defect levels in the SiO2/GaN interface. A significant shift in V th with additional light illumination was observed due to a charging of the defect states in the SiO2/GaN interface. The voltage shifts were attributed to the detrapping of defect states at the SiO2/GaN interface.
- Published
- 2018
- Full Text
- View/download PDF
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