25 results on '"STAT protein genetics"'
Search Results
2. Interferon-α Up-Regulates the Expression of PD-L1 Molecules on Immune Cells Through STAT3 and p38 Signaling.
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Bazhin, Alexandr V., von Ahn, Katharina, Fritz, Jasmin, Werner, Jens, and Karakhanova, Svetlana
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INTERFERONS ,PROGRAMMED cell death 1 receptors ,STAT protein genetics ,DENDRITIC cells ,IMMUNOSUPPRESSION ,CANCER immunotherapy - Abstract
Interferon-α (IFNα) has one of the longest histories of use amongst cytokines in clinical oncology and has been applied for the treatment of many types of cancers. Due to its immune-activating properties, IFNα is also an attractive candidate for combinatory anti-cancer therapies. Despite its extensive use in animal tumor models as well as in several clinical trials, the differentmechanisms underlying patient responses and affecting desirable clinical benefits are still under investigation. Here we show that in addition to its immune-activating properties, IFNα induces the expression of a key negative regulator, immunosuppressive PD-L1molecule, in themajority of the specific immune cell populations, particularly in the dendritic cells (DC). DC can modulate immune responses by a variety of mechanisms, including expression of T-cell regulatory molecules and cytokines. Our results showed that treatment of DC with IFNα-2b led to pronounced up-regulation of surface expression of PD-L1 molecules, increased IL-6 and decreased IL-12 production. Moreover, we present evidence that IFNα-treated DC exhibited a reduced capacity to stimulate interferon-γ production in T cells compared to control DC. This T-cell response after treatment of DC with IFNα was recovered by a pre-treatment with an anti-PD-L1 blocking antibody. Further analyses revealed that IFNα regulated PD-L1 expression through the STAT3 and p38 signaling pathways, since blocking of STAT3 and p38 activation with specific inhibitors prevented PD-L1 up-regulation. Our findings underline the important roles of p38 and STAT3 in the regulation of PD-L1 expression and prove that IFNα induces STAT3/p38-mediated expression of PD-L1 and thereby a reduced stimulatory ability of DC. The augmentation of PD-L1 expression in immune cells through IFNα treatment should be considered by use of IFNα in an anti-cancer therapy. [ABSTRACT FROM AUTHOR]
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- 2018
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3. STAT5-mediated chromatin interactions in superenhancers activate IL-2 highly inducible genes: Functional dissection of the Il2ra gene locus.
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Peng Li, Mitra, Suman, Spolski, Rosanne, Jangsuk Oh, Wei Liao, Zhonghui Tang, Fei Mo, Xingwang Li, West, Erin E., Gromer, Daniel, Jian-Xin Lin, Chengyu Liu, Yijun Ruan, and Leonard, Warren J.
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CYTOKINES , *STAT protein genetics , *T cells , *CHROMATIN , *INTERLEUKIN-2 - Abstract
Cytokines critically control immune responses, but how regulatory programs are altered to allow T cells to differentially respond to distinct cytokine stimuli remains poorly understood. Here, we have globally analyzed enhancer elements bound by IL-2-activated STAT5 and IL-21-activated STAT3 in T cells and identified Il2ra as the top-ranked gene regulated by an IL-2-activated STAT5-bound superenhancer and one of the top genes regulated by STAT3-bound superenhancers. Moreover, we found that STAT5 binding was rapidly superenriched at genes highly induced by IL-2 and that IL-2-activated STAT5 binding induces new and augmented chromatin interactions within superenhancer-containing genes. Based on chromatin interaction analysis by paired-end tag (ChIA-PET) sequencing data, we used CRISPR-Cas9 gene editing to target three of the STAT5 binding sites within the Il2ra superenhancer in mice. Each mutation decreased STAT5 binding and altered IL-2-induced Il2ra gene expression, revealing that individual elements within the superenhancer were not functionally redundant and that all were required for normal gene expression. Thus, we demonstrate cooperative utilization of superenhancer elements to optimize gene expression and show that STAT5 mediates IL-2-induced chromatin looping at superenhancers to preferentially regulate highly inducible genes, thereby providing new insights into the mechanisms underlying cytokine-dependent superenhancer function. [ABSTRACT FROM AUTHOR]
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- 2017
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4. Molecular cloning, transcriptional profiling, and subcellular localization of signal transducer and activator of transcription 2 (STAT2) ortholog from rock bream, Oplegnathus fasciatus.
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Bathige, S.D.N.K., Priyathilaka, Thanthrige Thiunuwan, Thulasitha, William Shanthakumar, Jayasinghe, J.D.H.E., Wan, Qiang, Lee, Jehee, Umasuthan, Navaneethaiyer, and Nam, Bo-Hye
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STAT protein genetics , *FISH genetics , *MOLECULAR cloning , *GENE expression profiling , *GENOMICS , *IMMUNE response , *ANTIVIRAL agent analysis , *INTERLEUKIN-10 , *GENETICS - Abstract
Signal transducer and activator of transcription 2 (STAT2) is a key element that transduces signals from the cell membrane to the nucleus via the type I interferon-signaling pathway. Although the structural and functional aspects of STAT proteins are well studied in mammals, information on teleostean STATs is very limited. In this study, a STAT paralog, which is highly homologous to the STAT2 members, was identified from a commercially important fish species called rock bream and designated as RbSTAT2. The RbSTAT2 gene was characterized at complementary DNA (cDNA) and genomic sequence levels, and was found to possess structural features common with its mammalian counterparts. The complete cDNA sequence was distributed into 24 exons in the genomic sequence. The promoter proximal region was analyzed and found to contain potential transcription factor binding sites to regulate the transcription of RbSTAT2 . Phylogenetic studies and comparative genomic structure organization revealed the distinguishable evolution for fish and other vertebrate STAT2 orthologs. Transcriptional quantification was performed by SYBR Green quantitative real-time PCR (qPCR) and the ubiquitous expression of RbSTAT2 transcripts was observed in all tissues analyzed from healthy fish, with a remarkably high expression in blood cells. Significantly ( P < 0.05) altered transcription of RbSTAT2 was detected after immune challenge experiments with viral (rock bream irido virus; RBIV), bacterial ( Edwardsiella tarda and Streptococcus iniae ), and immune stimulants (poly I:C and LPS). Antiviral potential was further confirmed by WST-1 assay, by measuring the viability of rock bream heart cells treated with RBIV. In addition, results of an in vitro challenge experiment signified the influence of rock bream interleukin-10 (RbIL-10) on transcription of RbSTAT2 . Subcellular localization studies by transfection of pEGFP-N1/RbSTAT2 into rock bream heart cells revealed that the RbSTAT2 was usually located in the cytoplasm and translocated near to the nucleus upon poly I:C administration. Altogether, these findings suggest that RbSTAT2 is involved in various biologically crucial mechanisms, and provides immune protection to the rock bream. [ABSTRACT FROM AUTHOR]
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- 2017
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5. Associations Between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 Polymorphisms and Fertility in Holstein-Friesian Heifers.
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ÖNER, Yasemin, YILMAZ, Onur, OKUT, Hayrettin, ATA, Nezih, YILMAZBAŞ-MECİTOĞLU, Gülnaz, and KESKİN, Abdulkadir
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HOLSTEIN-Friesian cattle , *GENETIC polymorphisms , *ARTIFICIAL insemination of cattle , *STAT protein genetics , *GENOTYPES , *REPRODUCTION - Abstract
In this study, it was aimed to investigate polymorphisms in seven genes (GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2) related to reproductive traits in dairy heifers. Frequency distributions of the genotypes between fertile and repeat breeder heifers groups were investigated. Allele effects on fertility were also analyzed. Blood samples were taken from a total of 160 Holstein-Friesian heifers and they were divided into two groups according to their artificial insemination numbers (AI). The heifers becoming pregnant after the first AI were used as the fertile heifers (FH, n=80) and the heifers with 3 or more equal AIs were accepted as the repeat breeder heifers (RBH, n=80). All the animals were genotyped by the PCR-RFLP method for seven genes and the association works were performed for 145 animals (RBH, n=79; FH n=66). For all loci investigated, two alleles and three genotypes were found for overall population with the exception that PRL locus had two alleles and two genotypes. The chi-square test (Χ²) revealed that the whole population and the two groups separately were at Hardy-Weinberg equilibrium. The genotype distributions of PIT-1 and STAT5A conspicuously differed between the FH and the RBH groups; however, these differences were not found significant. Association of GH-AB genotype was found significant on AI number for the first pregnancy. Mixed effect logistic regression model was used to investigate the allele effects on fertility. No linkage disequilibrium was detected between the investigated loci. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Identification of novel small molecules that inhibit STAT3-dependent transcription and function.
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Kolosenko, Iryna, Yu, Yasmin, Busker, Sander, Dyczynski, Matheus, Palm Apergi, Caroline, Tamm, Katja Pokrovskaja, Grander, Dan, Liu, Jianping, Haraldsson, Martin, Helleday, Thomas, and Page, Brent D. G.
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STAT protein genetics , *STAT proteins , *CANCER diagnosis , *GENE therapy , *CANCER treatment - Abstract
Activation of Signal Transducer and Activator of Transcription 3 (STAT3) has been linked to several processes that are critical for oncogenic transformation, cancer progression, cancer cell proliferation, survival, drug resistance and metastasis. Inhibition of STAT3 signaling has shown a striking ability to inhibit cancer cell growth and therefore, STAT3 has become a promising target for anti-cancer drug development. The aim of this study was to identify novel inhibitors of STAT-dependent gene transcription. A cellular reporter-based system for monitoring STAT3 transcriptional activity was developed which was suitable for high-throughput screening (Z’ = 0,8). This system was used to screen a library of 28,000 compounds (the ENAMINE Drug-Like Diversity Set). Following counter-screenings and toxicity studies, we identified four hit compounds that were subjected to detailed biological characterization. Of the four hits, KI16 stood out as the most promising compound, inhibiting STAT3 phosphorylation and transcriptional activity in response to IL6 stimulation. In silico docking studies showed that KI16 had favorable interactions with the STAT3 SH2 domain, however, no inhibitory activity could be observed in the STAT3 fluorescence polarization assay. KI16 inhibited cell viability preferentially in STAT3-dependent cell lines. Taken together, using a targeted, cell-based approach, novel inhibitors of STAT-driven transcriptional activity were discovered which are interesting leads to pursue further for the development of anti-cancer therapeutic agents. [ABSTRACT FROM AUTHOR]
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- 2017
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7. Dual regulation of Stat1 and Stat3 by the tumor suppressor protein PML contributes to interferon α-mediated inhibition of angiogenesis.
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Kuo-Sheng Hsu, Xuan Zhao, Xiwen Cheng, Dongyin Guan, Mahabeleshwar, Ganapati H., Yu Liu, Borden, Ernest, Jain, Mukesh K., and Hung-Ying Kao
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NEOVASCULARIZATION , *NEOVASCULARIZATION inhibitors , *STAT protein genetics , *TUMOR suppressor proteins , *INTERFERONS , *CELLULAR signal transduction , *THERAPEUTICS - Abstract
IFNs are effective in inhibiting angiogenesis in preclinical models and in treating several angioproliferative disorders. However, the detailed mechanisms of IFNα-mediated anti-angiogenesis are not completely understood. Stat1/2/3 and PML are IFNα downstream effectors and are pivotal regulators of angiogenesis. Here, we investigated PML's role in the regulation of Stat1/2/3 activity. In Pml knock-out (KO) mice, ablation of Pml largely reduces IFNα angiostatic ability in Matrigel plug assays. This suggested an essential role for PML in IFNα's antiangiogenic function. We also demonstrated that PML shared a large cohort of regulatory genes with Stat1 and Stat3, indicating an important role of PML in regulating Stat1 and Stat3 activity. Using molecular tools and primary endothelial cells, we demonstrated that PML positively regulates Stat1 and Stat2 isgylation, a ubiquitination-like protein modification. Accordingly, manipulation of the isgylation system by knocking down USP18 altered IFNα-PML axis-mediated inhibition of endothelial cell migration and network formation. Furthermore, PML promotes turnover of nuclear Stat3, and knockdown of PML mitigates the effect of LLL12, a selective Stat3 inhibitor, on IFNα-mediated anti-angiogenic activity. Taken together, we elucidated an unappreciated mechanism in which PML, an IFNα-inducible effector, possess potent angiostatic activity, doing so in part by forming a positive feedforward loop with Stat1/2 and a negative feedback loop with Stat3. The interplay between PML, Stat1/Stat2, and Stat3 contributes to IFNα-mediated inhibition of angiogenesis, and disruption of this network results in aberrant IFNα signaling and altered angiostatic activity. [ABSTRACT FROM AUTHOR]
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- 2017
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8. Volume Contents.
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ADRENALECTOMY , *STAT protein genetics , *TRNA-guanine transglycosylase - Published
- 2016
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9. Identification, gene expression and immune function of the novel Bm-STAT gene in virus-infected Bombyx mori.
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Zhang, Xiaoli, Guo, Rui, Kumar, Dhiraj, Ma, Huanyan, Liu, Jiabin, Hu, Xiaolong, Cao, Guangli, Xue, Renyu, and Gong, Chengliang
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STAT protein genetics , *SILKWORM diseases , *GENE expression , *INSECT genetics , *IMMUNITY , *INSECT virus diseases , *OPEN reading frames (Genetics) , *INSECTS - Abstract
Genes in the signal transducer and activator of transcription (STAT) family are vital for activities including gene expression and immune response. To investigate the functions of the silkworm Bombyx mori STAT ( Bm-STAT ) gene in antiviral immunity, two Bm-STAT gene isoforms, Bm-STAT -L for long form and Bm-STAT- S for short form, were cloned. Sequencing showed that the open reading frames were 2313 bp encoding 770 amino acid residues for Bm-STAT -L and 2202 bp encoding 734 amino acid residues for Bm-STAT- S. The C-terminal 42 amino acid residues of Bm-STAT-L were different from the last 7 amino acid residues of Bm-STAT-S. Immunofluorescence showed that Bm-STAT was primarily distributed in the nucleus. Transcription levels of Bm-STAT in different tissues were determined by quantitative PCR, and the results revealed Bm-STAT was mainly expressed in testes. Western blots showed two bands with molecular weights of 70 kDa and 130 kDa in testes, but no bands were detected in ovaries by using anti-Bm-STAT antibody as the primary antibody. Expression of Bm-STAT in hemolymph at 48 h post infection with B. mori macula-like virus (BmMLV) was slightly enhanced compared with controls, suggesting a weak response induced by infection with BmMLV. Hemocyte immunofluorescence showed that Bm-STAT expression was elevated in B. mori nucleopolyhedrovirus (BmNPV)-infected cells. Moreover, resistance of BmN cells to BmNPV was reduced by downregulation of Bm-STAT expression and increased by upregulation. Resistance of BmN cells to BmCPV was not significantly improved by upregulating Bm-STAT expression. Therefore, we concluded that Bm-STAT is a newly identified insect gene of the STAT family. The JAK-STAT pathway has a more specialized role in antiviral defense in silkworms, but JAK-STAT pathway is not triggered in response to all viruses. [ABSTRACT FROM AUTHOR]
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- 2016
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10. Expression of STAT5, COX-2 and PIAS3 in Correlation with NSCLC Histhopathological Features.
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Pastuszak-Lewandoska, Dorota, Domańska, Daria, Czarnecka, Karolina H., Kordiak, Jacek, Migdalska-Sęk, Monika, Nawrot, Ewa, Kiszałkiewicz, Justyna, Antczak, Adam, Górski, Paweł, and Brzeziańska, Ewa
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STAT protein genetics , *CYCLOOXYGENASE 2 , *LUNG cancer , *HISTOPATHOLOGY , *GENE expression , *MESSENGER RNA , *METASTASIS - Abstract
Signal transducers and activators of transcription (STATs), their inhibitors and cyclooxygenase-2 (COX-2) participate in transformations of many various types of cancers. The aim of the present study was to evaluate the relationship between STAT5A/B, COX-2, and PIAS3 mRNA expression and tumor staging, metastasis status, and histopathological subtype in 71 patients with confirmed non-small cell lung cancer (NSCLC) diagnosis. Total RNA was isolated from NSCLC tissue samples and the expression of the studied genes was assessed using TaqMan probes in real-time PCR assay. The expression levels of STAT5A, STAT5B, and COX-2 genes were increased in 69%, 79%, and 71% NSCLC samples respectively, while PIAS3 expression was decreased in the majority (69%) of the studied tissues. Statistically significant differences were observed between STAT5 isoforms (P = 0.0008), with higher expression of STAT5B. We found statistically significant positive correlation between STAT5B and COX-2 (rho = 0.045), and significant negative correlation between STAT5B and PIAS3 (rho = −0.049). The negative correlation between STAT5B and PIAS3 (rho = −0.43) was also observed in T2a+T2b tumor group. Additionally, STAT5B and COX-2 expression levels were significantly different between T1a+T1b and T2a+T2b tumors (P = 0.002 and P = 0.041, respectively), with higher expression of both genes in T2 tumor stage. PIAS3 expression was significantly lower in NSCC subtype as compared with SCC subtype (P = 0.017). Also, STAT5A and STAT5B immunoexpression was assessed, and the results indicated significantly higher protein levels in NSCLC patients as compared with controls (P = 0.048 and P = 0.034, respectively). High STAT5B immunoexpression was positively correlated with STAT5B gene expression in tumors (rho = 0.755). STAT5B protein level was also significantly higher in T2a+T2b tumors, reflecting high STAT5B gene expression in this group. There was no statistically significant association between mRNA and protein expression levels of the studied genes and patients' characteristics: age, gender, smoking. The obtained results highlight the importance of the genes STAT5B and COX-2 in lung cancer progression. [ABSTRACT FROM AUTHOR]
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- 2014
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11. The role of STAT-6 as a key transcription regulator in HeLa cell death induced by IFN-γ/TNF-α co-immobilized on nanoparticles.
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Li, Zhibin, Guan, Yan-Qing, and Liu, Jun-Ming
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STAT protein genetics , *GENETIC transcription , *GENETIC regulation , *HELA cells , *CELL death , *TUMOR necrosis factors , *CELLULAR signal transduction , *INTERFERONS - Abstract
Abstract: Based on the fact that the transcription of STAT-1 plus its Serine 727 and Tyrosine 701 phosphorylation is not the pre-requisite for the cell death signal transduction in the IFN-γ signaling pathway induced by co-immobilized IFN-γ/TNF-α, we investigate both in vitro and in vivo the key transcription regulators to promote the signal transduction of HeLa cells. It is found that IFN-γ R2 is the important death signal receptor in the HeLa cell death by RNA interference. Checking the expression of the whole transcription (STAT) protein family reveals that STAT-6 is highly expressed in comparison with the other STAT proteins. The gene silence of IFN-γ R2 leads to the down-regulation of STAT-6 and phosphorylation-STAT-6 (p-STAT-6) expressions. The successful gene silence of STAT-6 results in the reduction of HeLa cell programmed death and the expression of several important key factors related to programmed cell death (p53, Bcl-2, and Bax). More importantly, our in vivo experiments by injecting nanoparticle drug carriers with the co-immobilized IFN-γ/TNF-α into nude mice model confirm the high expression of STAT-6 and p-STAT-6. It is thus concluded that, in response to IFN-γ, the co-immobilized IFN-γ/TNF-α unusually promotes the activation of STAT-6 rather than STAT-1, resulting in the enhanced cell programmed death in HeLa. The present work reveals the gene-level molecular mechanism of IFN-γ/TNF-α co-immobilized on biomaterials as a potentially effective therapy against cancer cells. [Copyright &y& Elsevier]
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- 2014
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12. PTTG acts as a STAT3 target gene for colorectal cancer cell growth and motility.
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Zhou, C, Tong, Y, Wawrowsky, K, and Melmed, S
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GENETICS of colon cancer , *STAT protein genetics , *GENE targeting , *CANCER cell motility , *CANCER cell growth , *PITUITARY tumors , *TUMOR growth , *CANCER invasiveness - Abstract
Pituitary tumor-transforming gene (PTTG), the index mammalian securin, is abundantly expressed in several tumors and regulates tumor growth and progression. Molecular mechanisms elucidating PTTG regulation and actions remain elusive. Here, we provide evidence that PTTG acts as a signal transducer and activator of transcription factor 3 (STAT3) target gene. Total STAT3 and Tyr705 phosphorylated STAT3 were concordantly expressed with PTTG in human colorectal tumors (n=97 and n=95, respectively, P<0.001). STAT3 specifically bound the human PTTG promoter and induced PTTG transcriptional activity (twofold) as assessed by chromatin immunoprecipitation and luciferase reporter assays. STAT3 transfection increased PTTG mRNA and protein abundance twofold in HCT116 human colon cancer cells, and induction was further enhanced (threefold) by constitutively active STAT3 (STAT3-C), whereas strongly abrogated by dominant-negative STAT3 (STAT3-DN). Attenuating PTTG expression by siRNA in STAT3 HCT116 stable transfectants suppressed cell growth and colony formation in vitro, and PTTG cell knockout also constrained activated STAT3-induced explanted murine tumor growth in vivo. STAT3 increased HCT116 cell migration and invasion up to fivefold, whereas cell mobility was abolished by STAT3-DN (>85%). Impairing PTTG expression by siRNA also strongly suppressed STAT3-faciliated cell migration and invasion by up to 90%. Knocking out PTTG in STAT3-C HCT116 stable transfectants strongly decreased tumor metastases in nude mice, indicating the requirement of PTTG for STAT3-promoted metastasis. These results elucidate a mechanism for tumor cell PTTG regulation, whereby STAT3 induces PTTG expression to facilitate tumor growth and metastasis, and further support the rationale for targeting PTTG to abrogate colorectal cancer growth. [ABSTRACT FROM AUTHOR]
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- 2014
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13. Replication study of STAT4 rs7574865 G/T polymorphism and risk of rheumatoid arthritis in a Chinese population.
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Shen, Li, Liu, Ruiping, Zhang, Hui, Huang, Yong, Sun, Rongbin, and Tang, Peifu
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STAT protein genetics , *GENETIC polymorphisms , *GUANINE , *RHEUMATOID arthritis risk factors , *ANKYLOSING spondylitis , *GENETICS of autoimmune diseases , *INTERLEUKIN-12 genetics , *SINGLE nucleotide polymorphisms , *CHINESE people , *DISEASES - Abstract
Abstract: Aim: Rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are common systemic autoimmune diseases with genetic and environmental predisposing factors. Signal transducer and activator of transcription 4 (STAT4) transmits signals induced by interleukin-12, interleukin-23 and interferon-γ, which are key cytokines and play important roles in the development of autoimmune diseases. Previous studies confirmed the STAT4 rs7574865 G/T locus to be associated with RA. Thus we conducted a replication study to investigate STAT4 rs7574865 G/T polymorphism and RA/AS susceptibility in a Chinese population. Methods: We studied STAT4 rs7574865 G/T gene polymorphism in 520 patients with RA, 100 AS patients and 520 controls in a Chinese population. Genotyping was done using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results: When the STAT4 rs7574865 GG homozygote genotype was used as the reference group, the GT or GT/TT genotypes were associated with the risk for RA. After stratification analyses, a significantly increased risk for RA associated with the STAT4 rs7574865 GT genotype was evident among the rheumatoid factor (RF)-positive patients, patients with higher erythrocyte sedimentation rate (ESR) level and patients with higher RA disease activity score (DAS28) compared with the STAT4 rs7574865 GG genotype. A significantly increased risk for RA associated with the STAT4 rs7574865 TT genotype was evident among older patients and RF-negative patients compared with the STAT4 rs7574865 GG genotype. STAT4 rs7574865 G/T was not associated with susceptibility to AS. Conclusion: This replication study confirmed that STAT4 rs7574865 G/T polymorphism was associated with the risk of RA. Condensed abstract: STAT4 polymorphisms are associated with rheumatoid arthritis risk. [Copyright &y& Elsevier]
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- 2013
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14. Bioinformatics Method to Analyze the Mechanism of Pancreatic Cancer Disorder.
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Wang, Cong-Jun, Xu, Rong-Hua, Yuan, Qiong-Ying, Wang, Yong-Kun, Shen, Dong-Wei, Wang, Xu-Jing, Gao, Wei, Zhang, Hui, and Jiang, Hua
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PANCREATIC cancer genetics , *TRANSFORMING growth factors-beta , *STAT protein genetics , *GENE expression profiling , *GENETIC regulation , *FOCAL adhesion kinase , *COMPUTER simulation of biological systems - Abstract
Pancreatic cancer is an aggressive malignancy with a five-year mortality of 97-98% due to widespread metastatic disease. A better understanding of the molecular mechanism of pancreatic cancer is beneficial for the development of novel approaches for early detection and monitoring of pancreatic cancer. We aim to comprehensively identify the gene expression profile in pancreatic cancer and explore the molecular pathway of pancreatic cancer disorder. Using GSE15471 datasets downloaded from Gene Expression Omnibus data, we first screened the differentially expressed genes in pancreatic cancer using packages in R language. The key pathways of differentially expressed genes were investigated with the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and synergetic network construction based on weighted Jaccard index. A total of 13,211 differentially expressed genes were identified, and they were enriched in several pathways, such as mitogen-activated protein kinase (MAPK) signaling pathway, transforming growth factor (TGF)-beta signaling pathway, Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathway, and calcium signaling pathway, as well as cell cycle, focal adhesion, complement and coagulation cascades, and leukocyte transendothelial migration. Synergetic pathway network analysis revealed that cytokine-cytokine receptor interaction pathway, calcium signaling pathway, and focal adhesion pathway were three important pathways in the development of pancreatic cancer. The method introduced here is helpful to screen the key pathways for controlling pancreatic cancer progression and provide potential therapeutic targets in the treatment of pancreatic cancer. [ABSTRACT FROM AUTHOR]
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- 2013
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15. JAK-STAT pathway and myogenic differentiation.
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You-Na Jang and Eun Joo Baik
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STAT protein genetics , *KINASE genetics , *MYOBLASTS , *REGENERATION (Biology) , *IMMUNOSUPPRESSION , *CYTOKINES - Abstract
Myogenic differentiation plays an important role in muscle regeneration and is regulated by two transcription factor families, MRFs and MEF2, which induce differentiation of myoblasts through expression of the muscle-specific gene, myogenin. In addition, many intracellular signaling pathways are also involved in myogenic differentiation, including p38 MAPK, ERK/MAPK and PI3K/AKT. The JAK-STAT pathway is activated by various cytokines and positively or negatively regulates the differentiation of myoblasts. JAK1 plays a notable role in proliferation; whereas, JAK2 and JAK3 function mainly in differentiation. The STATs, molecules downstream of JAK, regulate myogenesis. With JAK1, STAT1 promotes proliferation, while STAT3 has a dual effect on proliferation and differentiation. The JAK-STAT negative regulator, SOCS, is also associated with myogenesis; although, its role is controversial. In this review, we will discuss the role of the JAK-STAT pathway on myogenic differentiation. [ABSTRACT FROM AUTHOR]
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- 2013
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16. Anti-STAT6 CTL activity in Stat6-/- mice A cautionary tale.
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Kaplan, Mark H., Cundiff, Judy K., Stader Smith, Jill, and Aldrich, Carla J.
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GERM cells , *STAT protein genetics , *KINASE genetics , *IMMUNOREGULATION , *THYMIC hormones , *T cells - Abstract
The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8+ T cells from Stat6-/- mice displayed "autoreactivity" to STAT6- expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6-/- mice, or where adoptive transfer of Stat6-/- lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice. [ABSTRACT FROM AUTHOR]
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- 2013
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17. STAT3 and the Hyper-IgE syndrome Clinical presentation, genetic origin, pathogenesis, novel findings and remaining uncertainties.
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Mogensen, Trine H.
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STAT protein genetics , *IMMUNODEFICIENCY , *KINASE genetics , *CARCINOGENESIS , *IMMUNOGLOBULIN E - Abstract
During recent years a number of primary immunodeficiencies resulting from impaired function of JAK-STAT molecules have been described. One of these is the Hyper-IgE syndrome (HIES) characterized by elevated IgE levels, eczema, recurrent staphylococcal skin and pulmonary infections and pleiotropic somatic manifestations. In 2007 the genetic basis of HIES was revealed by identification of dominant negative STAT3 mutations in HIES patients. Subsequently impaired function of Tyk2 and DOCK8 have been implicated in milder forms of HIES. Since STAT3 acts as a central transcription factor downstream of multiple cytokine and growth factor receptors and thus regulates antimicrobial responses and cell survival, impaired STAT3 function results in immunodeficiency and in some cases tumorigenesis. However, as the immunological and molecular basis of HIES is being unraveled, important biological and immunological insight into JAK-STAT signaling is emerging that may have implications for our understanding of the pathogenesis and clinical management of patients with HIES. [ABSTRACT FROM AUTHOR]
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- 2013
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18. STAT3 suppresses transcription of proapoptotic genes in cancer cells with the involvement of its N-terminal domain.
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Timofeeva, Olga A., Tarasova, Nadya I., Xueping Zhang, Chasovskikh, Sergey, Cheema, Amrita K., Honghe Wang, Brown, Milton L., and Dritschilo, Anatoly
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STAT protein genetics , *APOPTOTIC protease-activating factor 1 , *CANCER cell physiology , *GENETIC regulation , *NEOVASCULARIZATION inhibitors , *MOLECULAR structure of chromatin - Abstract
Activation of STAT3 in cancers leads to gene expression promoting cell proliferation and resistance to apoptosis, as well as tumor angiogenesis, invasion, and migration. In the characterization of effects of ST3-H2A2, a selective inhibitor of the STAT3 N-terminal domain (ND), we observed that the compound induced apoptotic death in cancer cells associated with robust activation of proapoptotic genes. Using ChIP and tiling human promoter arrays, we found that activation of gene expression in response to ST3-H2A2 is accompanied by altered STAT3 chromatin binding. Using inhibitors of STAT3 phosphorylation and a dominant-negative STAT3 mutant, we found that the unphosphorylated form of STAT3 binds to regulatory regions of proapoptotic genes and prevents their expression in tumor cells but not normal cells. siRNA knockdown confirmed the effects of ST3-HA2A on gene expression and chromatin binding to be STAT3 dependent. The STAT3-binding region of the C/EBP-homologous protein (CHOP) promoter was found to be localized in DNaseI hypersensitive site of chromatin in cancer cells but not in nontransformed cells, suggesting that STAT3 binding and suppressive action can be chromatin structure dependent. These data demonstrate a suppressive role for the STAT3 ND in the regulation of proapoptotic gene expression in cancer cells, providing further support for targeting STAT3 ND for cancer therapy. [ABSTRACT FROM AUTHOR]
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- 2013
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19. STAT heterodimers in immunity.
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Delgoffe, Greg M. and Vignali, Dario A. A.
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CYTOKINES , *IMMUNE response , *CELLULAR immunity , *STAT protein genetics , *CELL physiology - Abstract
Cytokine signals are essential for generating a robust and specialized immune response. These signals are typically transmitted via canonical STAT homodimers. However, the number of STAT molecules utilized by cytokine signaling cascades within immune cells are limited, and so the mechanism used to deliver complex signals remains elusive. Heterodimerization of STAT proteins is one potential mechanism for signals to be modified downstream of the receptor and may play an important role in dictating the targets of specific cytokine signaling. In this review, we discuss our current understanding of the prevalence of STAT heterodimers, how they are formed and what their physiologic role may be in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
20. STAT3 mutations unify the pathogenesis of chronic lymphoproliferative disorders of NK cells and T-cell large granular lymphocyte leukemia.
- Author
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Jerez, Andres, Clemente, Michael J., Makishima, Hideki, Koskela, Hanna, LeBlanc, Francis, Ng, Kwok Peng, Olson, Thomas, Przychodzen, Bartlomiej, Afable, Manuel, Gomez-Segui, Ines, Guinta, Kathryn, Durkin, Lisa, Hsi, Eric D., McGraw, Kathy, Zhang, Dan, Wlodarski, Marcin W., Porkka, Kimmo, Sekeres, Mikkael A., List, Alan, and Mustjoki, Satu
- Subjects
- *
LYMPHOPROLIFERATIVE disorders , *KILLER cells , *T-cell lymphoma , *LYMPHOCYTIC leukemia , *CYTOTOXIC T cells , *STAT protein genetics , *GENETIC load - Abstract
Chronic lymphoproliferative disorders of natural killer cells (CLPD-NKs) and T-cell large granular lymphocytic leukemias (T-LGLs) are clonal lymphoprollferations arising from either natural killer cells or cytotoxic T lymphocytes (CTLs). We have investigated for distribution and functional significance of mutations in 50 CLPD-NKs and 120 T-LGL patients by direct sequenc-ing, allele-specific PCR, and microarray analysis. STAT3 gene mutations are pres-ent in both T and NK diseases: approxi-mately one-third of patients with each type of disorder convey these mutations. Mutations were found in exons 21 and 20, encoding the Src homology 2 domain. Patients with mutations are characterized by symptomatic disease (75%), history of multiple treatments, and a specific pat-tern of STAT3 activation and gene deregu-lation, including increased expression of genes activated by STAT3. Many of these features are also found in patients with wild-type STAT3, Indicating that other mechanisms of STAT3 activation can be operative in these chronic lymphoprolif-erative disorders. Treatment with STAT3 Inhibitors, both in wild-type and mutant cases, resulted in accelerated apoptosis. STAT3 mutations are frequent in large granular lymphocytes suggesting a simi-lar molecular dysregulation in malignant chronic expansions of NK and CTL origin. STAT3 mutations may distinguish truly malignant lymphoproliferations Involving T and NK cells from reactive expansions. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
21. MiR-135a functions as a selective killer of malignant glioma.
- Author
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Wu, S, Lin, Y, Xu, D, Chen, J, Shu, M, Zhou, Y, Zhu, W, Su, X, Qiu, P, and Yan, G
- Subjects
- *
BRAIN tumor treatment , *MICRORNA , *STAT protein genetics , *GLIOMA treatment , *GENETIC regulation , *CELLULAR signal transduction , *NEUROGLIA , *ETIOLOGY of cancer - Abstract
Glioma is the most common and fatal primary brain tumor. Thus far, therapeutic strategies to efficiently and specifically antagonize glioma are limited and poorly developed. Here we report that glia-enriched miR-135a, a microRNA that is dramatically downregulated in malignant glioma and correlated with the pathological grading, is capable of inducing mitochondria-dependent apoptosis of malignant glioma by regulating various genes including STAT6, SMAD5 and BMPR2, as well as affecting the signaling pathway downstream. Moreover, this lethal effect is selectively towards malignant glioma cells, but not neurons and glial cells, through a novel mechanism. Our findings suggest an important role of miR-135a in glioma etiology and provide a potential candidate for malignant glioma therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
22. Advances in innate immune signaling: new activators and regulators.
- Author
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Juan Liu and Xuetao Cao
- Subjects
- *
GENETIC regulation , *IMMUNOLOGIC diseases , *STAT protein genetics , *GENETICS - Abstract
The article discusses research on advances in the identification and functional characterization of new activators and regulators of innate immune signaling pathways.
- Published
- 2016
- Full Text
- View/download PDF
23. A STAT3-based gene signature stratifies glioma patients for targeted therapy.
- Author
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Chong, Yuk Kien, Lim, See Wee, Tan, Melanie Si Yan, Koh, Lynnette Wei Hsien, Sandanaraj, Edwin, Tang, Carol, Tan, Nguan Soon, Ang, Beng Ti, Ng, Wai Hoe, and Tan, Patrick
- Subjects
GLIOMAS ,GLIOMA treatment ,STAT protein genetics ,MICE ,GENETIC transcription - Abstract
Intratumoral heterogeneity is a hallmark of glioblastoma (GBM) tumors, thought to negatively influence therapeutic outcome. Previous studies showed that mesenchymal tumors have a worse outcome than the proneural subtype. Here we focus on STAT3 as its activation precedes the proneural-mesenchymal transition. We first establish a STAT3 gene signature that stratifies GBM patients into STAT3-high and -low cohorts. STAT3 inhibitor treatment selectively mitigates STAT3-high cell viability and tumorigenicity in orthotopic mouse xenograft models. We show the mechanism underlying resistance in STAT3-low cells by combining STAT3 signature analysis with kinome screen data on STAT3 inhibitor-treated cells. This allows us to draw connections between kinases affected by STAT3 inhibitors, their associated transcription factors and target genes. We demonstrate that dual inhibition of IGF-1R and STAT3 sensitizes STAT3-low cells and improves survival in mice. Our study underscores the importance of serially profiling tumors so as to accurately target individuals who may demonstrate molecular subtype switching. STAT3 activates distinct transcriptional programmes within cancer cells. In this study, the authors find that, in glioma, a STAT3-mediated expression signature can stratify patients for targeted precision therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
24. Role of STAT3 in lung cancer.
- Author
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Dutta, Pranabananda, Li, Jinghong, Li, Willis X, and Sabri, Nafiseh
- Subjects
- *
LUNG cancer , *STAT protein genetics , *HUMAN stem cells , *STEM cell research , *TUMOR suppressor proteins - Abstract
Lung cancer remains a challenging disease. It is responsible for the high cancer mortality rates in the US and worldwide. Elucidation of the molecular mechanisms operative in lung cancer is an important first step in developing effective therapies. Accumulating evidence over the last 2 decades suggests a critical role for Signal Transducer and Activator of Transcription 3 (STAT3) as a point of convergence for various signaling pathways that are dysregulated in the disease. In this review, we discuss possible molecular mechanisms involving STAT3 in lung tumorigenesis based on recent literature. We consider possible roles of STAT3 in cancer cell proliferation and survival, in the tumor immune environment, and in epigenetic regulation and interaction of STAT3 with other transcription factors. We also discuss the potential role of STAT3 in tumor suppression, which complicates strategies of targeting STAT3 in cancer therapy. [ABSTRACT FROM PUBLISHER]
- Published
- 2014
- Full Text
- View/download PDF
25. 1083-191 Biphasic regulation of STAT1 alpha expression in vascular smooth muscle cells by oxidized low-density lipoprotein.
- Author
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Ono, Koichi, Sukhanov, Sergiy, Hua Song, Yao, and Delafontaine, Patrick
- Subjects
- *
STAT protein genetics , *VASCULAR smooth muscle , *LOW density lipoproteins , *GENETIC regulation , *OXIDATION of proteins , *CARDIAC research - Published
- 2004
- Full Text
- View/download PDF
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