1. PIP
- Author
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Katherine M, Stefanski, Charles M, Russell, Justin M, Westerfield, Rajan, Lamichhane, and Francisco N, Barrera
- Subjects
Phosphatidylinositol 4,5-Diphosphate ,Protein Conformation ,OCD, oriented circular dichroism ,FRET, Förster resonance energy transfer ,HR, heptad repeat ,juxtamembrane ,JM, juxtamembrane ,TIRF, total internal reflection fluorescence ,Protein Domains ,Humans ,GZ, glycine zipper ,TEM, transmission electron microscopy ,ANOVA, analysis of variance ,SMALP ,Binding Sites ,Receptor, EphA2 ,AKT ,Cell Membrane ,SMALPs, styrene maleic acid lipid particles ,ALS, amyotrophic lateral sclerosis ,PS, phosphatidylserine ,MD, molecular dynamics ,transmembrane ,DTT, dithiothreitol ,HPLC, high-performance liquid chromatography ,receptor tyrosine kinase ,single-molecule ,Protein Multimerization ,MALDI-TOF, matrix-assisted laser desorption ionization time of flight ,RTK, receptor tyrosine kinase ,Protein Binding ,Signal Transduction ,Research Article ,TM, transmembrane - Abstract
The impact of the EphA2 receptor on cancer malignancy hinges on the two different ways it can be activated. EphA2 induces antioncogenic signaling after ligand binding, but ligand-independent activation of EphA2 is pro-oncogenic. It is believed that the transmembrane (TM) domain of EphA2 adopts two alternate conformations in the ligand-dependent and the ligand-independent states. However, it is poorly understood how the difference in TM helical crossing angles found in the two conformations impacts the activity and regulation of EphA2. We devised a method that uses hydrophobic matching to stabilize two conformations of a peptide comprising the EphA2 TM domain and a portion of the intracellular juxtamembrane (JM) segment. The two conformations exhibit different TM crossing angles, resembling the ligand-dependent and ligand-independent states. We developed a single-molecule technique using styrene maleic acid lipid particles to measure dimerization in membranes. We observed that the signaling lipid PIP2 promotes TM dimerization, but only in the small crossing angle state, which we propose corresponds to the ligand-independent conformation. In this state the two TMs are almost parallel, and the positively charged JM segments are expected to be close to each other, causing electrostatic repulsion. The mechanism PIP2 uses to promote dimerization might involve alleviating this repulsion due to its high density of negative charges. Our data reveal a conformational coupling between the TM and JM regions and suggest that PIP2 might directly exert a regulatory effect on EphA2 activation in cells that is specific to the ligand-independent conformation of the receptor.
- Published
- 2020