1. Prenyl Pterocarpans from Algerian Bituminaria bituminosa and Their Effects on Neuroblastoma.
- Author
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Benhabrou H, Bitam F, Cristino L, Nicois A, Carbone M, Ammar D, Gavagnin M, and Ciavatta ML
- Subjects
- Humans, Cell Line, Tumor, Cell Proliferation drug effects, Molecular Structure, Plant Extracts chemistry, Plant Extracts pharmacology, Apoptosis drug effects, Neuroblastoma pathology, Neuroblastoma drug therapy, Pterocarpans chemistry, Pterocarpans pharmacology, Pterocarpans isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification
- Abstract
The pterocarpan fraction from aerial parts of Bituminaria bituminosa was investigated for both chemical characterization and biological evaluation. Chemical studies were in accordance with the literature data on Bituminaria genus resulting in the identification of typical 4,8-prenyl pterocarpans. Three new members, bituminarins A-C ( 1 - 3 ), were isolated along with main bitucarpin A ( 4 ), erybraedin C ( 5 ) and erybraedin D ( 6 ) already reported from this plant. Further, biological studies evidenced antiproliferative properties of the most abundant pterocarpans 4 and 5 on neuroblastoma SH-SY5Y cell line, in agreement with previously described antiproliferative activity of these compounds against cancer cell lines other than neuroblastoma. The structure and the stereochemistry of the new molecules was determined by extensive spectroscopic analysis and chemical derivatization methods. The biological investigation was carried out by using an assay platform based on a live-cell imaging system revealing an apoptotic cell death induction.
- Published
- 2024
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