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1. Bendable non-silicon RISC-V microprocessor

Author

Ozer, Emre, Kufel, Jedrzej, Prakash, Shvetank, Raisiardali, Alireza, Kindgren, Olof, Wong, Ronald, Ng, Nelson, Jausseran, Damien, Alkhalil, Feras, Kong, David, Hills, Gage, Price, Richard, and Reddi, Vijay Janapa

Published
2024
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2. Binary Black Hole Coalescence Phenomenology from Numerical Relativity

Author

Price, Richard H., Bachhar, Ritesh, and Khanna, Gaurav

Subjects
General Relativity and Quantum Cosmology
Abstract

The major source of ground-based gravitational wave detectors, the inspiral and merger of comparable mass binary black holes (BBH), consists of a slow quasicircular inspiral, a merger to form a single remnant hole, and the quasinormal ringing of that remnant. The first and last of these epochs are amenable to well developed and familiar approximations: Newtonian or post-Newtonian for the first, and BH perturbation methods for the last. Ironically, the middle epoch, the merger, generates most of the GW emission, yet has been accessible so far only to numerical relativity. Here we add the close-limit approximation for the phenomenology of the merger. With the completed set of methods (Newtonian/post-Newtonian; close limit; BH perturbation theory) we show that not only can we understand the results of BBH coalescence, but - with reasonable accuracy - we can {\it predict} the resulting radiation and remnant., Comment: 10 pages, 12 figures, 2 tables

Published
2023

3. Bookshelf 2023

Author

Price, Richard and Price, Sally

Published
2024

6. Angular Momentum for Black Hole Binaries in Numerical Relativity

Author

Bachhar, Ritesh, Price, Richard, and Khanna, Gaurav

Subjects
General Relativity and Quantum Cosmology
Abstract

The extensive catalog of waveforms, with details of binary black hole inspiral and merger, offer an opportunity to understand black hole interactions beyond the large separation regime. We envision a research program that focuses on the transfer of angular momentum from spin of the individual holes to the orbital angular momentum and the role of tidal coupling in the process. That analysis will require the formulation of an expression for the orbital angular momentum of a binary, an expression that is useful at small separations, since that regime is well out of the range of Newtonian approximations and is where tidal coupling should be most interesting. We report here such an expression, a binary orbital angular momentum based on numerical relativity results for quasi-circular orbits, that agrees remarkably well with a similar quantity constructed with particle-perturbation techniques for the Kerr geometry., Comment: 8 pages, 5 figures, 3 tables

Published
2023

9. Rebound HIV-1 in cerebrospinal fluid after antiviral therapy interruption is mainly clonally amplified R5 T cell-tropic virus

Author

Kincer, Laura P, Joseph, Sarah Beth, Gilleece, Maria M, Hauser, Blake M, Sizemore, Sabrina, Zhou, Shuntai, Di Germanio, Clara, Zetterberg, Henrik, Fuchs, Dietmar, Deeks, Steven G, Spudich, Serena, Gisslen, Magnus, Price, Richard W, and Swanstrom, Ronald

Subjects
Microbiology, Biological Sciences, Neurosciences, Infectious Diseases, HIV/AIDS, Pediatric AIDS, Pediatric, 2.2 Factors relating to the physical environment, Aetiology, Infection, Good Health and Well Being, Humans, T-Lymphocytes, HIV-1, HIV Infections, Central Nervous System, Antiviral Agents, Medical Microbiology
Abstract

HIV-1 persists as a latent reservoir in people receiving suppressive antiretroviral therapy (ART). When ART is interrupted (treatment interruption/TI), rebound virus re-initiates systemic infection in the lymphoid system. During TI, HIV-1 is also detected in cerebrospinal fluid (CSF), although the source of this rebound virus is unknown. To investigate whether there is a distinct HIV-1 reservoir in the central nervous system (CNS), we compared rebound virus after TI in the blood and CSF of 11 participants. Peak rebound CSF viral loads vary and we show that high viral loads and the appearance of clonally amplified viral lineages in the CSF are correlated with the transient influx of white blood cells. We found no evidence of rebound macrophage-tropic virus in the CSF, even in one individual who had macrophage-tropic HIV-1 in the CSF pre-therapy. We propose a model in which R5 T cell-tropic virus is released from infected T cells that enter the CNS from the blood (or are resident in the CNS during therapy), with clonal amplification of infected T cells and virus replication occurring in the CNS during TI.

Published
2023

10. Issues in the comparison of particle perturbations and numerical relativity for binary black hole mergers

Author

Price, Richard and Khanna, Gaurav

Subjects
General Relativity and Quantum Cosmology
Abstract

Recent work on improved efficiency of calculations for extreme mass ratio inspirals has produced the useful byproduct of comparisons of inspirals of comparable mass by particle perturbation (PP) methods and by numerical relativty (NR). Here we point out: (1) In choosing the rescaling of the masses, consideration must be given to the differences in the PP and NR methods even in the earliest, least nonlinear regime; in particular barycenter effects must be addressed. (2) Care must be given to the comparison of the nonspinning remnant in PP and the rapidly spinning remnant in NR., Comment: 6 pages, 2 figures, 2 tables (accepted for publication in PRD)

Published
2022

11. Alumni Networks Reimagined: Innovations Expanding Alumni Connections to Improve Postsecondary Pathways

Author

Clayton Christensen Institute for Disruptive Innovation, Fisher, Julia Freeland, and Price, Richard

Abstract

Alumni networks are part of the bill of goods that colleges and universities sell to students. But according to a Strada-Gallup Alumni Survey, only 9% of college graduates reported that their alumni network was helpful or very helpful in the job market. That startling statistic says a lot about how poorly most institutions actually perform when it comes to systematically connecting alumni and students in reliable, scalable ways. Endowment-supported institutions have long-held, elaborate strategies in place to "engage" alumni to mine their financial capital. But if the Strada-Gallup alumni survey is any clue, they--and their peer institutions with smaller or no endowments--could be doing more to successfully activate alumni's social capital. That social capital has immense potential to drive improvements in students' experiences while on campus, and to bolster graduates' long-term return on investment (ROI) in a degree. With the right tools and models, postsecondary institutions could enlist alumni to deliberately equip college-goers with career skills and professional networks. This report highlights innovative companies and models that are emerging to help postsecondary players harness their alumni networks in new ways, and provides an analysis of five ways that rethinking alumni connections could fulfill postsecondary education's promise. The tools and models highlighted in this paper reflect the fact that it's networks--not just degrees--that lead to jobs. Alumni networks are a critical resource as postsecondary programs look to enrich the student experience, particularly with an eye towards boosting graduates' ROI.

Published
2021

12. Stratified analyses refine association between TLR7 rare variants and severe COVID-19

Author

Rimoldi, Valeria, Paraboschi, Elvezia M., Bandera, Alessandra, Peyvandi, Flora, Grasselli, Giacomo, Blasi, Francesco, Malvestiti, Francesco, Pelusi, Serena, Bianco, Cristiana, Miano, Lorenzo, Lombardi, Angela, Invernizzi, Pietro, Gerussi, Alessio, Citerio, Giuseppe, Biondi, Andrea, Valsecchi, Maria Grazia, Cazzaniga, Marina Elena, Foti, Giuseppe, Beretta, Ilaria, D'Angiò, Mariella, Bettini, Laura Rachele, Farré, Xavier, Iraola-Guzmán, Susana, Kogevinas, Manolis, Castaño-Vinyals, Gemma, Garcia-Etxebarria, Koldo, Nafria, Beatriz, D'Amato, Mauro, Palom, Adriana, Begg, Colin, Clohisey, Sara, Hinds, Charles, Horby, Peter, Knight, Julian, Ling, Lowell, Maslove, David, McAuley, Danny, Millar, Johnny, Montgomery, Hugh, Nichol, Alistair, Openshaw, Peter J.M., Pereira, Alexandre C., Ponting, Chris P., Rowan, Kathy, Semple, Malcolm G., Shankar-Hari, Manu, Summers, Charlotte, Walsh, Timothy, Baillie, J. Kenneth, Aravindan, Latha, Armstrong, Ruth, Biggs, Heather, Boz, Ceilia, Brown, Adam, Clark, Richard, Coutts, Audrey, Coyle, Judy, Cullum, Louise, Das, Sukamal, Day, Nicky, Donnelly, Lorna, Duncan, Esther, Fawkes, Angie, Fineran, Paul, Fourman, Max Head, Furlong, Anita, Furniss, James, Gallagher, Bernadette, Gilchrist, Tammy, Golightly, Ailsa, Griffiths, Fiona, Hafezi, Katarzyna, Hamilton, Debbie, Hendry, Ross, Law, Andy, Law, Dawn, Law, Rachel, Law, Sarah, Lidstone-Scott, Rebecca, Macgillivray, Louise, Maclean, Alan, Mal, Hanning, McCafferty, Sarah, Mcmaster, Ellie, Meikle, Jen, Moore, Shona C., Morrice, Kirstie, Murphy, Lee, Murphy, Sheena, Hellen, Mybaya, Oosthuyzen, Wilna, Zheng, Chenqing, Chen, Jiantao, Parkinson, Nick, Paterson, Trevor, Schon, Katherine, Stenhouse, Andrew, Das, Mihaela, Swets, Maaike, Szoor-McElhinney, Helen, Taneski, Filip, Turtle, Lance, Wackett, Tony, Ward, Mairi, Weaver, Jane, Wrobel, Nicola, Zechner, Marie, Arbane, Gill, Bociek, Aneta, Campos, Sara, Grau, Neus, Jones, Tim Owen, Lim, Rosario, Marotti, Martina, Ostermann, Marlies, Whitton, Christopher, Alldis, Zoe, Astin-Chamberlain, Raine, Bibi, Fatima, Biddle, Jack, Blow, Sarah, Bolton, Matthew, Borra, Catherine, Bowles, Ruth, Burton, Maudrian, Choudhury, Yasmin, Collier, David, Cox, Amber, Easthope, Amy, Ebano, Patrizia, Fotiadis, Stavros, Gurasashvili, Jana, Halls, Rosslyn, Hartridge, Pippa, Kallon, Delordson, Kassam, Jamila, Lancoma-Malcolm, Ivone, Matharu, Maninderpal, May, Peter, Mitchelmore, Oliver, Newman, Tabitha, Patel, Mital, Pheby, Jane, Pinzuti, Irene, Prime, Zoe, Prysyazhna, Oleksandra, Shiel, Julian, Taylor, Melanie, Tierney, Carey, Wood, Suzanne, Zak, Anne, Zongo, Olivier, Bonner, Stephen, Hugill, Keith, Jones, Jessica, Liggett, Steven, Headlam, Evie, Bandla, Nageswar, Gellamucho, Minnie, Davies, Michelle, Thompson, Christopher, Abdelrazik, Marwa, Bakthavatsalam, Dhanalakshmi, Elhassan, Munzir, Ganesan, Arunkumar, Haldeos, Anne, Moreno-Cuesta, Jeronimo, Purohit, Dharam, Vincent, Rachel, Xavier, Kugan, Rohit, Kumar, Alasdair, Frater, Saleem, Malik, David, Carter, Samuel, Jenkins, Lamond, Zoe, Alanna, Wall, Fernandez-Roman, Jaime, Hamilton, David O., Johnson, Emily, Johnston, Brian, Martinez, Maria Lopez, Mulla, Suleman, Shaw, David, Waite, Alicia A.C., Waugh, Victoria, Welters, Ingeborg D., Williams, Karen, Cavazza, Anna, Cockrell, Maeve, Corcoran, Eleanor, Depante, Maria, Finney, Clare, Jerome, Ellen, McPhail, Mark, Nayak, Monalisa, Noble, Harriet, O'Reilly, Kevin, Pappa, Evita, Saha, Rohit, Saha, Sian, Smith, John, Knighton, Abigail, Antcliffe, David, Banach, Dorota, Brett, Stephen, Coghlan, Phoebe, Fernandez, Ziortza, Gordon, Anthony, Rojo, Roceld, Arias, Sonia Sousa, Templeton, Maie, Meredith, Megan, Morris, Lucy, Ryan, Lucy, Clark, Amy, Sampson, Julia, Peters, Cecilia, Dent, Martin, Langley, Margaret, Ashraf, Saima, Wei, Shuying, Andrew, Angela, Bashyal, Archana, Davidson, Neil, Hutton, Paula, McKechnie, Stuart, Wilson, Jean, Baptista, David, Crowe, Rebecca, Fernandes, Rita, Herdman-Grant, Rosaleen, Joseph, Anna, O'Connor, Denise, Allen, Meryem, Loveridge, Adam, McKenley, India, Morino, Eriko, Naranjo, Andres, Simms, Richard, Sollesta, Kathryn, Swain, Andrew, Venkatesh, Harish, Khera, Jacyntha, Fox, Jonathan, Andrew, Gillian, Barclay, Lucy, Callaghan, Marie, Campbell, Rachael, Clark, Sarah, Hope, Dave, Marshall, Lucy, McCulloch, Corrienne, Briton, Kate, Singleton, Jo, Birch, Sohphie, Brimfield, Lutece, Daly, Zoe, Pogson, David, Rose, Steve, Battle, Ceri, Brinkworth, Elaine, Harford, Rachel, Murphy, Carl, Newey, Luke, Rees, Tabitha, Williams, Marie, Arnold, Sophie, Polgarova, Petra, Stroud, Katerina, Meaney, Eoghan, Jones, Megan, Ng, Anthony, Agrawal, Shruti, Pathan, Nazima, White, Deborah, Daubney, Esther, Elston, Kay, Grauslyte, Lina, Hussain, Musarat, Phull, Mandeep, Pogreban, Tatiana, Rosaroso, Lace, Salciute, Erika, Franke, George, Wong, Joanna, George, Aparna, Ortiz-Ruiz de Gordoa, Laura, Peasgood, Emily, Phillips, Claire, Bates, Michelle, Dasgin, Jo, Gill, Jaspret, Nilsson, Annette, Scriven, James, Delgado, Carlos Castro, Dawson, Deborah, Ding, Lijun, Durrant, Georgia, Ezeobu, Obiageri, Farnell-Ward, Sarah, Harrison, Abiola, Kanu, Rebecca, Leaver, Susannah, Maccacari, Elena, Manna, Soumendu, Saluzzio, Romina Pepermans, Queiroz, Joana, Samakomva, Tinashe, Sicat, Christine, Texeira, Joana, Da Gloria, Edna Fernandes, Lisboa, Ana, Rawlins, John, Mathew, Jisha, Kinch, Ashley, Hurt, William James, Shah, Nirav, Clark, Victoria, Thanasi, Maria, Yun, Nikki, Patel, Kamal, Bennett, Sara, Goodwin, Emma, Jackson, Matthew, Kent, Alissa, Tibke, Clare, Woodyatt, Wiesia, Zaki, Ahmed, Abraheem, Azmerelda, Bamford, Peter, Cawley, Kathryn, Dunmore, Charlie, Faulkner, Maria, Girach, Rumanah, Jeffrey, Helen, Jones, Rhianna, London, Emily, Nagra, Imrun, Nasir, Farah, Sainsbury, Hannah, Smedley, Clare, Patel, Tahera, Smith, Matthew, Chukkambotla, Srikanth, Kazi, Aayesha, Hartley, Janice, Dykes, Joseph, Hijazi, Muhammad, Keith, Sarah, Khan, Meherunnisa, Ryan-Smith, Janet, Springle, Philippa, Thomas, Jacqueline, Truman, Nick, Saad, Samuel, Coleman, Dabheoc, Fine, Christopher, Matt, Roseanna, Gay, Bethan, Dalziel, Jack, Ali, Syamlan, Goodchild, Drew, Harling, Rhiannan, Bhatterjee, Ravi, Goddard, Wendy, Davison, Chloe, Duberly, Stephen, Hargreaves, Jeanette, Bolton, Rachel, Davey, Miriam, Golden, David, Seaman, Rebecca, Cherian, Shiney, Cutler, Sean, Heron, Anne Emma, Roynon-Reed, Anna, Szakmany, Tamas, Williams, Gemma, Richards, Owen, Cheema, Yusuf, Brooke, Hollie, Buckley, Sarah, Suarez, Jose Cebrian, Charlesworth, Ruth, Hansson, Karen, Norris, John, Poole, Alice, Rose, Alastair, Sandhu, Rajdeep, Sloan, Brendan, Smithson, Elizabeth, Thirumaran, Muthu, Wagstaff, Veronica, Metcalfe, Alexandra, Brunton, Mark, Caterson, Jess, Coles, Holly, Frise, Matthew, Rai, Sabi Gurung, Jacques, Nicola, Keating, Liza, Tilney, Emma, Bartley, Shauna, Bhuie, Parminder, Gibson, Sian, Lyle, Amanda, McNeela, Fiona, Radhakrishnan, Jayachandran, Hughes, Alistair, Yates, Bryan, Reynolds, Jessica, Campbell, Helen, Thompsom, Maria, Dodds, Steve, Duffy, Stacey, Greer, Sandra, Shuker, Karen, Tridente, Ascanio, Khade, Reena, Sundar, Ashok, Tsinaslanidis, George, Birkinshaw, Isobel, Carter, Joseph, Howard, Kate, Ingham, Joanne, Joy, Rosie, Pearson, Harriet, Roche, Samantha, Scott, Zoe, Bancroft, Hollie, Bellamy, Mary, Carmody, Margaret, Daglish, Jacqueline, Moore, Faye, Rhodes, Joanne, Sangombe, Mirriam, Kadiri, Salma, Croft, Maria, White, Ian, Frost, Victoria, Aquino, Maia, Jha, Rajeev, Krishnamurthy, Vinodh, Lim, Lai, Lim, Li, Combes, Edward, Joefield, Teishel, Monnery, Sonja, Beech, Valerie, Trotman, Sallyanne, Almaden-Boyle, Christine, Austin, Pauline, Cabrelli, Louise, Cole, Stephen, Casey, Matt, Chapman, Susan, Whyte, Clare, Baird, Yolanda, Butler, Aaron, Chadbourn, Indra, Folkes, Linda, Fox, Heather, Gardner, Amy, Gomez, Raquel, Hobden, Gillian, Hodgson, Luke, King, Kirsten, Margarson, Michael, Martindale, Tim, Meadows, Emma, Raynard, Dana, Thirlwall, Yvette, Helm, David, Margalef, Jordi, Criste, Kristine, Cusack, Rebecca, Golder, Kim, Golding, Hannah, Jones, Oliver, Leggett, Samantha, Male, Michelle, Marani, Martyna, Prager, Kirsty, Williams, Toran, Roberts, Belinda, Salmon, Karen, Anderson, Peter, Archer, Katie, Austin, Karen, Davis, Caroline, Durie, Alison, Kelsall, Olivia, Thrush, Jessica, Vigurs, Charlie, Wild, Laura, Wood, Hannah-Louise, Tranter, Helen, Harrison, Alison, Cowley, Nicholas, McAlindon, Michael, Burtenshaw, Andrew, Digby, Stephen, Low, Emma, Morgan, Aled, Cother, Naiara, Rankin, Tobias, Clayton, Sarah, McCurdy, Alex, Ahmed, Cecilia, Baines, Balvinder, Clamp, Sarah, Colley, Julie, Haq, Risna, Hayes, Anne, Hulme, Jonathan, Hussain, Samia, Joseph, Sibet, Kumar, Rita, Maqsood, Zahira, Purewal, Manjit, Benham, Leonie, Bradshaw, Zena, Brown, Joanna, Caswell, Melanie, Cupitt, Jason, Melling, Sarah, Preston, Stephen, Slawson, Nicola, Stoddard, Emma, Warden, Scott, Deacon, Bethan, Lynch, Ceri, Pothecary, Carla, Roche, Lisa, Howe, Gwenllian Sera, Singh, Jayaprakash, Turner, Keri, Ellis, Hannah, Stroud, Natalie, Hunt, Jodie, Dearden, Joy, Dobson, Emma, Drummond, Andy, Mulcahy, Michelle, Munt, Sheila, O'Connor, Grainne, Philbin, Jennifer, Rishton, Chloe, Tully, Redmond, Winnard, Sarah, Cathcart, Susanne, Duffy, Katharine, Puxty, Alex, Puxty, Kathryn, Turner, Lynne, Ireland, Jane, Semple, Gary, Long, Kate, Whiteley, Simon, Wilby, Elizabeth, Ogg, Bethan, Cowton, Amanda, Kay, Andrea, Kent, Melanie, Potts, Kathryn, Wilkinson, Ami, Campbell, Suzanne, Brown, Ellen, Melville, Julie, Naisbitt, Jay, Joseph, Rosane, Lazo, Maria, Walton, Olivia, Neal, Alan, Alexander, Peter, Allen, Schvearn, Bradley-Potts, Joanne, Brantwood, Craig, Egan, Jasmine, Felton, Timothy, Padden, Grace, Ward, Luke, Moss, Stuart, Glasgow, Susannah, Abel, Lynn, Brett, Michael, Digby, Brian, Gemmell, Lisa, Hornsby, James, MacGoey, Patrick, O'Neil, Pauline, Price, Richard, Rodden, Natalie, Rooney, Kevin, Sundaram, Radha, Thomson, Nicola, Hopkins, Bridget, Thrasyvoulou, Laura, Willis, Heather, Clark, Martyn, Coulding, Martina, Jude, Edward, McCormick, Jacqueline, Mercer, Oliver, Potla, Darsh, Rehman, Hafiz, Savill, Heather, Turner, Victoria, Downes, Charlotte, Holding, Kathleen, Riches, Katie, Hilton, Mary, Hayman, Mel, Subramanian, Deepak, Daniel, Priya, Adanini, Oluronke, Bhatia, Nikhil, Msiska, Maines, Collins, Rebecca, Clement, Ian, Patel, Bijal, Gulati, A., Hays, Carole, Webster, K., Hudson, Anne, Webster, Andrea, Stephenson, Elaine, McCormack, Louise, Slater, Victoria, Nixon, Rachel, Hanson, Helen, Fearby, Maggie, Kelly, Sinead, Bridgett, Victoria, Robinson, Philip, Camsooksai, Julie, Humphrey, Charlotte, Jenkins, Sarah, Reschreiter, Henrik, Wadams, Beverley, Death, Yasmin, Bastion, Victoria, Clarke, Daphene, David, Beena, Kent, Harriet, Lorusso, Rachel, Lubimbi, Gamu, Murdoch, Sophie, Penacerrada, Melchizedek, Thomas, Alastair, Valentine, Jennifer, Vochin, Ana, Wulandari, Retno, Djeugam, Brice, Bell, Gillian, English, Katy, Katary, Amro, Wilcox, Louise, Bruce, Michelle, Connolly, Karen, Duncan, Tracy, T-Michael, Helen, Lindergard, Gabriella, Hey, Samuel, Fox, Claire, Alfonso, Jordan, Durrans, Laura Jayne, Guerin, Jacinta, Blackledge, Bethan, Harris, Jade, Hruska, Martin, Eltayeb, Ayaa, Lamb, Thomas, Hodgkiss, Tracey, Cooper, Lisa, Rothwell, Joanne, Allan, Angela, Anderson, Felicity, Kaye, Callum, Liew, Jade, Medhora, Jasmine, Scott, Teresa, Trumper, Erin, Botello, Adriana, Lankester, Liana, Nikitas, Nikitas, Wells, Colin, Stowe, Bethan, Spencer, Kayleigh, Brandwood, Craig, Smith, Lara, Birchall, Katie, Kolakaluri, Laurel, Baines, Deborah, Sukumaran, Anila, Apetri, Elena, Basikolo, Cathrine, Catlow, Laura, Charles, Bethan, Dark, Paul, Doonan, Reece, Harvey, Alice, Horner, Daniel, Knowles, Karen, Lee, Stephanie, Lomas, Diane, Lyons, Chloe, Marsden, Tracy, McLaughlan, Danielle, McMorrow, Liam, Pendlebury, Jessica, Perez, Jane, Poulaka, Maria, Proudfoot, Nicola, Slaughter, Melanie, Slevin, Kathryn, Thomas, Vicky, Walker, Danielle, Michael, Angiy, Collis, Matthew, Cosier, Tracey, Millen, Gemma, Richardson, Neil, Schumacher, Natasha, Weston, Heather, Rand, James, Baxter, Nicola, Henderson, Steven, Kennedy-Hay, Sophie, McParland, Christopher, Rooney, Laura, Sim, Malcolm, McCreath, Gordan, Akeroyd, Louise, Bano, Shereen, Bromley, Matt, Gurr, Lucy, Lawton, Tom, Morgan, James, Sellick, Kirsten, Warren, Deborah, Wilkinson, Brian, McGowan, Janet, Ledgard, Camilla, Stacey, Amelia, Pye, Kate, Bellwood, Ruth, Bentley, Michael, Bewley, Jeremy, Garland, Zoe, Grimmer, Lisa, Gumbrill, Bethany, Johnson, Rebekah, Sweet, Katie, Webster, Denise, Efford, Georgia, Convery, Karen, Fottrell-Gould, Deirdre, Hudig, Lisa, Keshet-Price, Jocelyn, Randell, Georgina, Stammers, Katie, Bokhari, Maria, Linnett, Vanessa, Lucas, Rachael, McCormick, Wendy, Ritzema, Jenny, Sanderson, Amanda, Wild, Helen, Rostron, Anthony, Roy, Alistair, Woods, Lindsey, Cornell, Sarah, Wakinshaw, Fiona, Rogerson, Kimberley, Jarmain, Jordan, Parker, Robert, Reddy, Amie, Turner-Bone, Ian, Wilding, Laura, Harding, Peter, Abernathy, Caroline, Foster, Louise, Gratrix, Andrew, Martinson, Vicky, Parkinson, Priyai, Stones, Elizabeth, Carbral-Ortega, Llucia, Bercades, Georgia, Brealey, David, Hass, Ingrid, MacCallum, Niall, Martir, Gladys, Raith, Eamon, Reyes, Anna, Smyth, Deborah, Zitter, Letizia, Benyon, Sarah, Marriott, Suzie, Park, Linda, Keenan, Samantha, Gordon, Elizabeth, Quinn, Helen, Baines, Kizzy, Cagova, Lenka, Fofano, Adama, Garner, Lucie, Holcombe, Helen, Mepham, Sue, Mitchell, Alice Michael, Mwaura, Lucy, Praman, Krithivasan, Vuylsteke, Alain, Zamikula, Julie, Purewal, Bally, Rivers, Vanessa, Bell, Stephanie, Blakemore, Hayley, Borislavova, Borislava, Faulkner, Beverley, Gendall, Emma, Goff, Elizabeth, Hayes, Kati, Thomas, Matt, Worner, Ruth, Smith, Kerry, Stephens, Deanna, Mew, Louise, Mwaura, Esther, Stewart, Richard, Williams, Felicity, Wren, Lynn, Sutherland, Sara-Beth, Bevan, Emily, Martin, Jane, Trodd, Dawn, Watson, Geoff, Brown, Caroline Wrey, Collins, Amy, Khaliq, Waqas, Gude, Estefania Treus, Akinkugbe, Olugbenga, Bamford, Alasdair, Beech, Emily, Belfield, Holly, Bell, Michael, Davies, Charlene, Jones, Gareth A.L., McHugh, Tara, Meghari, Hamza, O'Neill, Lauran, Peters, Mark J., Ray, Samiran, Tomas, Ana Luisa, Burn, Iona, Hambrook, Geraldine, Manso, Katarina, Penn, Ruth, Shanmugasundaram, Pradeep, Tebbutt, Julie, Thornton, Danielle, Cole, Jade, Davies, Rhys, Duffin, Donna, Hill, Helen, Player, Ben, Thomas, Emma, Williams, Angharad, Griffin, Denise, Muchenje, Nycola, Mupudzi, Mcdonald, Partridge, Richard, Conyngham, Jo-Anna, Thomas, Rachel, Wright, Mary, Corral, Maria Alvarez, Jacob, Reni, Jones, Cathy, Denmade, Craig, Beavis, Sarah, Dale, Katie, Gascoyne, Rachel, Hawes, Joanne, Pritchard, Kelly, Stevenson, Lesley, Whileman, Amanda, Doble, Patricia, Hutter, Joanne, Pawley, Corinne, Shovelton, Charmaine, Vaida, Marius, Butcher, Deborah, O'Sullivan, Susie, Butterworth-Cowin, Nicola, Ahmad, Norfaizan, Barker, Joann, Bauchmuller, Kris, Bird, Sarah, Cawthron, Kay, Harrington, Kate, Jackson, Yvonne, Kibutu, Faith, Lenagh, Becky, Masuko, Shamiso, Mills, Gary H., Raithatha, Ajay, Wiles, Matthew, Willson, Jayne, Newell, Helen, Lye, Alison, Nwafor, Lorenza, Jarman, Claire, Rowland-Jones, Sarah, Foote, David, Cole, Joby, Thompson, Roger, Watson, James, Hesseldon, Lisa, Macharia, Irene, Chetam, Luke, Smith, Jacqui, Ford, Amber, Anderson, Samantha, Birchall, Kathryn, Housley, Kay, Walker, Sara, Milner, Leanne, Hanratty, Helena, Trower, Helen, Phillips, Patrick, Oxspring, Simon, Donne, Ben, Jardine, Catherine, Williams, Dewi, Hay, Alasdair, Flanagan, Rebecca, Hughes, Gareth, Latham, Scott, McKenna, Emma, Anderson, Jennifer, Hull, Robert, Rhead, Kat, Cruz, Carina, Pattison, Natalie, Charnock, Rob, McFarland, Denise, Cosgrove, Denise, Ahmed, Ashar, Morris, Anna, Jakkula, Srinivas, Ali, Asifa, Brady, Megan, Dale, Sam, Dance, Annalisa, Gledhill, Lisa, Greig, Jill, Hanson, Kathryn, Holdroyd, Kelly, Home, Marie, Kelly, Diane, Kitson, Ross, Matapure, Lear, Melia, Deborah, Mellor, Samantha, Nortcliffe, Tonicha, Pinnell, Jez, Robinson, Matthew, Shaw, Lisa, Shaw, Ryan, Thomis, Lesley, Wilson, Alison, Wood, Tracy, Bayo, Lee-Ann, Merwaha, Ekta, Ishaq, Tahira, Hanley, Sarah, Hibbert, Meg, Tetla, Dariusz, Woodford, Chrsitopher, Durga, Latha, Kennard-Holden, Gareth, Branney, Debbie, Frankham, Jordan, Pitts, Sally, White, Nigel, Laha, Shondipon, Verlander, Mark, Williams, Alexandra, Altabaibeh, Abdelhakim, Alvaro, Ana, Gilbert, Kayleigh, Ma, Louise, Mostoles, Loreta, Parmar, Chetan, Simpson, Kathryn, Jetha, Champa, Booker, Lauren, Pratley, Anezka, Adams, Colene, Agasou, Anita, Arden, Tracie, Bowes, Amy, Boyle, Pauline, Beekes, Mandy, Button, Heather, Capps, Nigel, Carnahan, Mandy, Carter, Anne, Childs, Danielle, Donaldson, Denise, Hard, Kelly, Hurford, Fran, Hussain, Yasmin, Javaid, Ayesha, Jones, James, Jose, Sanal, Leigh, Michael, Martin, Terry, Millward, Helen, Motherwell, Nichola, Rikunenko, Rachel, Stickley, Jo, Summers, Julie, Ting, Louise, Tivenan, Helen, Tonks, Louise, Wilcox, Rebecca, Holland, Maureen, Keenan, Natalie, Lyons, Marc, Wassall, Helen, Marsh, Chris, Mahenthran, Mervin, Carter, Emma, Kong, Thomas, Blackman, Helen, Creagh-Brown, Ben, Donlon, Sinead, Michalak-Glinska, Natalia, Mtuwa, Sheila, Pristopan, Veronika, Salberg, Armorel, Smith, Eleanor, Stone, Sarah, Piercy, Charles, Verula, Jerik, Burda, Dorota, Montaser, Rugia, Harden, Lesley, Mayangao, Irving, Marriott, Cheryl, Bradley, Paul, Harris, Celia, Anderson, Susan, Andrews, Eleanor, Birch, Janine, Collins, Emma, Hammerton, Kate, O'Leary, Ryan, Clark, Michele, Purvis, Sarah, Barber, Russell, Hewitt, Claire, Hilldrith, Annette, Jackson-Lawrence, Karen, Shepardson, Sarah, Wills, Maryanne, Butler, Susan, Tavares, Silvia, Cunningham, Amy, Hindale, Julia, Arif, Sarwat, Bean, Sarah, Burt, Karen, Spivey, Michael, Demetriou, Carrie, Eckbad, Charlotte, Hierons, Sarah, Howie, Lucy, Mitchard, Sarah, Ramos, Lidia, Serrano-Ruiz, Alfredo, White, Katie, Kelly, Fiona, Cristiano, Daniele, Dormand, Natalie, Farzad, Zohreh, Gummadi, Mahitha, Liyanage, Kamal, Patel, Brijesh, Salmi, Sara, Sloane, Geraldine, Thwaites, Vicky, Varghese, Mathew, Zborowski, Anelise C., Allan, John, Geary, Tim, Houston, Gordon, Meikle, Alistair, O'Brien, Peter, Forsey, Miranda, Kaliappan, Agilan, Nicholson, Anne, Riches, Joanne, Vertue, Mark, Allan, Elizabeth, Darlington, Kate, Davies, Ffyon, Easton, Jack, Kumar, Sumit, Lean, Richard, Menzies, Daniel, Pugh, Richard, Qiu, Xinyi, Davies, Llinos, Williams, Hannah, Scanlon, Jeremy, Davies, Gwyneth, Mackay, Callum, Lewis, Joannne, Rees, Stephanie, Oblak, Metod, Popescu, Monica, Thankachen, Mini, Higham, Andrew, Simpson, Kerry, Craig, Jayne, Baruah, Rosie, Morris, Sheila, Ferguson, Susie, Shepherd, Amy, Prockter Moore, Luke Stephen, Vizcaychipi, Marcela Paola, Gomes de Almeida Martins, Laura, Carungcong, Jaime, Mohamed Ali, Inthakab Ali, Beaumont, Karen, Blunt, Mark, Coton, Zoe, Curgenven, Hollie, Elsaadany, Mohamed, Fernandes, Kay, Ally, Sameena Mohamed, Rangarajan, Harini, Sarathy, Varun, Selvanayagam, Sivarupan, Vedage, Dave, White, Matthew, Gill, Mandy, Paul, Paul, Ratnam, Valli, Shelton, Sarah, Wynter, Inez, Carmody, Siobhain, Page, Valerie Joan, Beith, Claire Marie, Black, Karen, Clements, Suzanne, Morrison, Alan, Strachan, Dominic, Taylor, Margaret, Clarkson, Michelle, D'Sylva, Stuart, Norman, Kathryn, Auld, Fiona, Donnachie, Joanne, Edmond, Ian, Prentice, Lynn, Runciman, Nikole, Salutous, Dario, Symon, Lesley, Todd, Anne, Turner, Patricia, Short, Abigail, Sweeney, Laura, Murdoch, Euan, Senaratne, Dhaneesha, Hill, Michaela, Kannan, Thogulava, Laura, Wild, Crawley, Rikki, Crew, Abigail, Cunningham, Mishell, Daniels, Allison, Harrison, Laura, Hope, Susan, Inweregbu, Ken, Jones, Sian, Lancaster, Nicola, Matthews, Jamie, Nicholson, Alice, Wray, Gemma, Langton, Helen, Prout, Rachel, Watters, Malcolm, Novis, Catherine, Barron, Anthony, Collins, Ciara, Kaul, Sundeep, Passmore, Heather, Prendergast, Claire, Reed, Anna, Rogers, Paula, Shokkar, Rajvinder, Woodruff, Meriel, Middleton, Hayley, Polgar, Oliver, Nolan, Claire, Mahay, Kanta, Collier, Dawn, Hormis, Anil, Maynard, Victoria, Graham, Cheryl, Walker, Rachel, Knights, Ellen, Price, Alicia, Thomas, Alice, Thorpe, Chris, Behan, Teresa, Burnett, Caroline, Hatton, Jonathan, Heeney, Elaine, Mitra, Atideb, Newton, Maria, Pollard, Rachel, Stead, Rachael, Amin, Vishal, Anastasescu, Elena, Anumakonda, Vikram, Karthik, Komala, Kausar, Rizwana, Reid, Karen, Smith, Jacqueline, Imeson-Wood, Janet, Skinner, Denise, Gaylard, Jane, Mullan, Dee, Newman, Julie, Brown, Alison, Crickmore, Vikki, Debreceni, Gabor, Wilkins, Joy, Nicol, Liz, Reece-Anthony, Rosie, Birt, Mark, Ghosh, Alison, Williams, Emma, Allen, Louise, Beranova, Eva, Crisp, Nikki, Deery, Joanne, Hazelton, Tracy, Knight, Alicia, Price, Carly, Tilbey, Sorrell, Turki, Salah, Turney, Sharon, Cooper, Joshua, Finch, Cheryl, Liderth, Sarah, Quinn, Alison, Waddington, Natalia, Coventry, Tina, Fowler, Susan, MacMahon, Michael, McGregor, Amanda, Cowley, Anne, Highgate, Judith, Gregory, Jane, O'Connell, Susan, Smith, Tim, Barberis, Luigi, Gopal, Shameer, Harris, Nichola, Lake, Victoria, Metherell, Stella, Radford, Elizabeth, Daniel, Amelia, Finn, Joanne, Saha, Rajnish, White, Nikki, Donnison, Phil, Trim, Fiona, Eapen, Beena, Birch, Jenny, Bough, Laura, Goodsell, Josie, Tutton, Rebecca, Williams, Patricia, Williams, Sarah, Winter-Goodwin, Barbara, Nichol, Ailstair, Brickell, Kathy, Smyth, Michelle, Murphy, Lorna, Coetzee, Samantha, Gales, Alistair, Otahal, Igor, Raj, Meena, Sell, Craig, Hilltout, Paula, Evitts, Jayne, Tyler, Amanda, Waldron, Joanne, Beesley, Kate, Board, Sarah, Kubisz-Pudelko, Agnieszka, Lewis, Alison, Perry, Jess, Pippard, Lucy, Wood, Di, Buckley, Clare, Barry, Peter, Flint, Neil, Rekha, Patel, Hales, Dawn, Bunni, Lara, Jennings, Claire, Latif, Monica, Marshall, Rebecca, Subramanian, Gayathri, McGuigan, Peter J., Wasson, Christopher, Finn, Stephanie, Green, Jackie, Collins, Erin, King, Bernadette, Campbell, Andy, Smuts, Sara, Duffield, Joseph, Smith, Oliver, Mallon, Lewis, Claire, Watkins, Botfield, Liam, Butler, Joanna, Dexter, Catherine, Fletcher, Jo, Garg, Atul, Kuravi, Aditya, Ranga, Poonam, Virgilio, Emma, Belagodu, Zakaula, Fuller, Bridget, Gherman, Anca, Olufuwa, Olumide, Paramsothy, Remi, Stuart, Carmel, Oakley, Naomi, Kamundi, Charlotte, Tyl, David, Collins, Katy, Silva, Pedro, Taylor, June, King, Laura, Coates, Charlotte, Crowley, Maria, Wakefield, Phillipa, Beadle, Jane, Johnson, Laura, Sargeant, Janet, Anderson, Madeleine, Brady, Ailbhe, Chan, Rebekah, Little, Jeff, McIvor, Shane, Prady, Helena, Whittle, Helen, Mathew, Bijoy, Attwood, Ben, Parsons, Penny, Ward, Geraldine, Bremmer, Pamela, Joe, West, Tracy, Baird, Jim, Ruddy, Davies, Ellie, Sathe, Sonia, Dennis, Catherine, McGregor, Alastair, Parris, Victoria, Srikaran, Sinduya, Sukha, Anisha, Clarke, Noreen, Whiteside, Jonathan, Mascarenhas, Mairi, Donaldson, Avril, Matheson, Joanna, Barrett, Fiona, O'Hara, Marianne, Okeefe, Laura, Bradley, Clare, Eastgate-Jackson, Christine, Filipe, Helder, Martin, Daniel, Maharajh, Amitaa, Garcia, Sara Mingo, Pakou, Glykeria, De Neef, Mark, Dent, Kathy, Horsley, Elizabeth, Akhtar, Muhmmad Nauman, Pearson, Sandra, Potoczna, Dorota, Spencer, Sue, Clapham, Melanie, Harper, Rosemary, Poultney, Una, Rice, Polly, Mutch, Rachel, Armstrong, Lisa, Bates, Hayley, Dooks, Emma, Farquhar, Fiona, Hairsine, Brigid, McParland, Chantal, Packham, Sophie, Bi, Rehana, Scholefield, Barney, Ashton, Lydia, George, Linsha, Twiss, Sophie, Wright, David, Chablani, Manish, Kirkby, Amy, Netherton, Kimberley, Davies, Kim, O'Brien, Linda, Omar, Zohra, Perkins, Emma, Lewis, Tracy, Sutherland, Isobel, Burns, Karen, Ben Chandler, Dr, Elliott, Kerry, Mallinson, Janine, Turnbull, Alison, Gondo, Prisca, Hadebe, Bernard, Kayani, Abdul, Masunda, Bridgett, Anderson, Taya, Hawcutt, Dan, O'Malley, Laura, Rad, Laura, Rogers, Naomi, Saunderson, Paula, Allison, Kathryn Sian, Afolabi, Deborah, Whitbread, Jennifer, Jones, Dawn, Dore, Rachael, Halkes, Matthew, Mercer, Pauline, Thornton, Lorraine, Dawson, Joy, Garrioch, Sweyn, Tolson, Melanie, Aldridge, Jonathan, Kapoor, Ritoo, Loader, David, Castle, Karen, Humphreys, Sally, Tampsett, Ruth, Mackintosh, Katherine, Ayers, Amanda, Harrison, Wendy, North, Julie, Allibone, Suzanne, Genetu, Roman, Kasipandian, Vidya, Patel, Amit, Mac, Ainhi, Murphy, Anthony, Mahjoob, Parisa, Nazari, Roonak, Worsley, Lucy, Fagan, Andrew, Bemand, Thomas, Black, Ethel, Dela Rosa, Arnold, Howle, Ryan, Jhanji, Shaman, Baikady, Ravishankar Rao, Tatham, Kate Colette, Thomas, Benjamin, Bell, Dina, Boyle, Rosalind, Douglas, Katie, Glass, Lynn, Lee, Emma, Lennon, Liz, Rattray, Austin, Taylor, Abigail, Hughes, Rachel Anne, Thomas, Helen, Rees, Alun, Duskova, Michaela, Phipps, Janet, Brooks, Suzanne, Edwards, Michelle, Quaid, Sheena, Watson, Ekaterina, Brayne, Adam, Fisher, Emma, Hunt, Jane, Jackson, Peter, Kaye, Duncan, Love, Nicholas, Parkin, Juliet, Tuckey, Victoria, Van Koutrik, Lynne, Carter, Sasha, Andrew, Benedict, Findlay, Louise, Adams, Katie, Service, Jen, Williams, Alison, Cheyne, Claire, Saunderson, Anne, Moultrie, Sam, Odam, Miranda, Hall, Kathryn, Mapfunde, Isheunesu, Willis, Charlotte, Lyon, Alex, Sri-Chandana, Chunda, Scherewode, Joslan, Stephenson, Lorraine, Marsh, Sarah, Hardy, John, Houlden, Henry, Moncur, Eleanor, Tariq, Ambreen, Tucci, Arianna, Hobrok, Maria, Loosley, Ronda, McGuinness, Heather, Tench, Helen, Wolf-Roberts, Rebecca, Irvine, Val, Shelley, Benjamin, Gorman, Claire, Gupta, Abhinav, Timlick, Elizabeth, Brady, Rebecca, Milligan, Barry, Bellini, Arianna, Bryant, Jade, Mayer, Anton, Pickard, Amy, Roe, Nicholas, Sowter, Jason, Howlett, Alex, Fidler, Katy, Tagliavini, Emma, Donnelly, Kevin, Boos, Jannik, van der Made, Caspar I., Ramakrishnan, Gayatri, Coughlan, Eamon, Asselta, Rosanna, Löscher, Britt-Sabina, Valenti, Luca V.C., de Cid, Rafael, Bujanda, Luis, Julià, Antonio, Pairo-Castineira, Erola, May, Sandra, Zametica, Berina, Heggemann, Julia, Albillos, Agustín, Banales, Jesus M., Barretina, Jordi, Blay, Natalia, Bonfanti, Paolo, Buti, Maria, Fernandez, Javier, Marsal, Sara, Prati, Daniele, Ronzoni, Luisa, Sacchi, Nicoletta, Schultze, Joachim L., Riess, Olaf, Franke, Andre, Rawlik, Konrad, Ellinghaus, David, Hoischen, Alexander, Schmidt, Axel, and Ludwig, Kerstin U.

Published
2024
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13. Reply to ‘Cognitive criteria in HIV: greater consensus is needed’

Author

Nightingale, Sam, Cinque, Paola, Dravid, Ameet, Dreyer, Anna J., Gisslén, Magnus, Joska, John A., Kwasa, Judith, Meyer, Ana-Claire, Mpongo, Nombeko, Nakasujja, Noeline, Pebody, Roger, Pozniak, Anton, Price, Richard W., Saylor, Deanna, Thomas, Kevin G. F., Underwood, Jonathan, Vera, Jaime H., and Winston, Alan

Published
2024
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15. Creating Seamless Credit Transfer: A Parallel Higher Ed System to Support America through and beyond the Recession

Author

Clayton Christensen Institute for Disruptive Innovation, Horn, Michael B., and Price, Richard

Abstract

As COVID-19 wreaks havoc on institutions and the students they serve, ushering in a recession that could have existential implications for all, helping learners earn the credentials they need from the providers best suited to serve them will be a critical issue in determining how well and robustly the nation recovers. The federal government has already provided increased financial support to postsecondary students and institutions. More stimulus funds are likely on the way, offering a rare opportunity to not just shore up existing models, but also to provide a much-needed jolt to the calcified collegiate system, and to rethink how its players interact. Directing additional funds toward interoperability issues could go a long way in alleviating credit transfer challenges. In order to tackle higher education's credit transfer challenge, the Department of Education, along with state departments of higher education, should foster a parallel higher education system in which they support third-party credentialing entities that validate industry-valued skills. Regulators would use additional dollars that are focused on training students for in-demand jobs in this time of rampant unemployment, such as expanded Pell grants, to fund these efforts. This move away from an institution-centered posture to one in which third-party bodies are the assessors of quality and gatekeepers of credentials would skirt the debates about whether learning at one institution is equivalent to that of another. Such a learner-centered approach, focused on the accumulation of knowledge and skills, would facilitate seamless transfer without credit loss, shift significant portions of higher education from seat-time to competency-based learning, and help learners transition expeditiously back into the workforce.

Published
2020

17. Bookshelf 2022

Author

Price, Richard and Price, Sally

Published
2023

18. Elevated Cerebrospinal Fluid Anti-CD4 Autoantibody Levels in HIV Associate with Neuroinflammation

Author

Cheng, Da, Luo, Zhenwu, Fu, Xiaoyu, Stephenson, Sophie, Di Germanio, Clara, Norris, Philip J, Fuchs, Dietmar, Ndhlovu, Lishomwa C, Li, Quan-zhen, Zetterberg, Henrik, Gisslen, Magnus, Price, Richard W, Peng, Shifang, and Jiang, Wei

Subjects
Neurosciences, HIV/AIDS, Clinical Research, Aetiology, 2.1 Biological and endogenous factors, Infection, Adult, Autoantigens, Biomarkers, CD4 Antigens, Central Nervous System, Cytokines, Female, HIV Infections, Humans, Immunoglobulin G, Male, Middle Aged, Neurofilament Proteins, Neuroinflammatory Diseases, HIV-1 infection, neuroinflammation, anti-CD4 IgG, cerebrospinal fluid, HIV, anti-CD4 autoantibody
Abstract

The mechanisms of persistent central nervous system (CNS) inflammation in people with HIV (PWH) despite effective antiretroviral therapy (ART) are not fully understood. We have recently shown that plasma anti-CD4 IgGs contribute to poor CD4+ T cell recovery during suppressive ART via antibody-mediated cytotoxicity (ADCC) against CD4+ T cells, and that plasma anti-CD4 IgG levels are associated with worse cognitive performance and specific brain area atrophy. However, the role of anti-CD4 IgGs in neuroinflammation remains unclear. In the current study, plasma and cerebrospinal fluid (CSF) samples from 31 ART-naive and 26 treated, virologically suppressed PWH, along with 16 HIV-seronegative controls, were evaluated for CSF levels of anti-CD4 IgG, white blood cell (WBC) counts, soluble biomarkers of neuroinflammation, and neurofilament light chain (NfL). We found that 37% of the PWH exhibited elevated CSF anti-CD4 IgG levels, but few or none of the PWH were observed with elevated CSF anti-CD4 IgM, anti-CD8 IgG, or anti-double-strand DNA IgG. CSF anti-CD4 IgG levels in PWH were directly correlated with neuroinflammation (WBC counts, neopterin, and markers of myeloid cell activation), but not with CSF NfL levels. Using cells from one immune nonresponder to ART, we generated a pathogenic anti-CD4 monoclonal IgG (JF19) presenting with ADCC activity; JF19 induced the production of soluble CD14 (sCD14) and interleukin-8 (IL-8) in human primary monocyte-derived macrophages via CD4 binding in vitro. This study demonstrates for the first time that elevated CSF anti-CD4 IgG levels present in a subgroup of PWH which may play a role in neuroinflammation in HIV. IMPORTANCE This study reports that an autoantibody presents in the CNS of HIV patients and that its levels in the CSF correlate with some markers of neuroinflammation.

Published
2022

20. In situ tumour arrays reveal early environmental control of cancer immunity

Author

Ortiz-Muñoz, Guadalupe, Brown, Markus, Carbone, Catherine B., Pechuan-Jorge, Ximo, Rouilly, Vincent, Lindberg, Henrik, Ritter, Alex T., Raghupathi, Gautham, Sun, Qianbo, Nicotra, Tess, Mantri, Shreya R., Yang, Angela, Doerr, Jonas, Nagarkar, Deepti, Darmanis, Spyros, Haley, Benjamin, Mariathasan, Sanjeev, Wang, Yulei, Gomez-Roca, Carlos, de Andrea, Carlos Eduardo, Spigel, David, Wu, Thomas, Delamarre, Lelia, Schöneberg, Johannes, Modrusan, Zora, Price, Richard, Turley, Shannon J., Mellman, Ira, and Moussion, Christine

Published
2023
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21. Unlocking the Potential of Income Share Agreements (ISAs) to Tackle the Student Debt Crisis

Author

Clayton Christensen Institute for Disruptive Innovation and Price, Richard

Abstract

With one million defaults on student loans every year, and $1.6 trillion in outstanding student debt, it's clear the US is in desperate need of innovative funding models in higher education. Income Share Agreements, or ISAs, stand to provide a promising alternative to high-risk student loans, as they better align the interests of students, schools, and lenders. In an ISA, students pay no tuition upfront, only repaying the education provider once employed--in essence, funding today's educational opportunities for a fixed percentage of tomorrow's income, within a set window of time. In other words, an ISA is an equity investment, whereas a student loan is a debt burden. ISAs thus serve as a viable choice for students seeking to finance their education, and in many cases, increase access to higher education by helping students afford programs not eligible for federal financial aid. Despite the potential of the ISA model, a lack of regulatory clarity has limited its adoption. To reach that potential, the ISA market needs guardrails that both protect students and encourage investment in the space. In this policy brief, the authors provide an overview of ISAs and examples of ISAs currently in operation. They then explore guardrails that are essential to effective ISA legislation and analyze the ramifications of these provisions for students, education providers, and other ISA market participants. In so doing, the authors hope to empower policymakers as they unlock the power of a financing tool that has the potential to revolutionize higher education. [Contributions to this report were made by Alana Dunagan.]

Published
2019

22. Betting on Bootcamps: How Short-Course Training Programs Could Change the Landscape of Higher Ed

Author

Clayton Christensen Institute for Disruptive Innovation, Price, Richard, and Dunagan, Alana

Abstract

Whirlwind growth in the technology sector has led to heightened demand for workers with specialized skills in coding and computer science. Projections for continued expansion of the sector feed a persistent fear that traditional educational offerings won't generate enough graduates with the skills the economy demands. Bootcamps focused on coding and computer science have emerged as an important pipeline for tech talent. Studying the bootcamp model through the lens of Disruption Theory highlights its disruptive potential relative to traditional higher education. Bootcamps are addressing nonconsumption and overserved demographics with a product that is arguably inferior to traditional degrees--but one that is also simpler and cheaper. Bootcamps are leveraging technology for skills-based signaling, expanding their online presence, and seeing little response from traditional institutions. But, whether bootcamps disrupt higher education depends on whether and how federal funds enter the market, and on bootcamps' ability to expand into lifelong learning and beyond the technology sector. The authors identified five scenarios for how the future of bootcamps could play out: (1) Bootcamps get stuck and fail to disrupt higher education; (2) Federal funds could open up access to bootcamps--or destroy the model entirely; (3) Bootcamps expand into lifelong learning; (4) Bootcamps expand into industries beyond tech; and (5) Bootcamps achieve breadth and depth, and widespread disruption. Traditional institutions can integrate professional and technical skills into their programs, but this will not protect them from disruption. A more foolproof way to address this disruptive threat is to invest in the bootcamp business model through an autonomous unit. Successfully pushing into new industries and new training contexts will require bootcamps to continuously innovate. But if they take on that innovation challenge successfully, bootcamp models could disrupt higher education and dramatically and permanently change the landscape of education and training.

Published
2019

24. Cerebrospinal Fluid and Plasma Lipopolysaccharide Levels in Human Immunodeficiency Virus Type 1 Infection and Associations With Inflammation, Blood-Brain Barrier Permeability, and Neuronal Injury

Author

Jiang, Wei, Luo, Zhenwu, Stephenson, Sophie, Li, Hong, Di Germanio, Clara, Norris, Philip J, Fuchs, Dietmar, Zetterberg, Henrik, Gisslen, Magnus, and Price, Richard W

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Infectious Diseases, Acquired Cognitive Impairment, Neurosciences, HIV/AIDS, Brain Disorders, 2.1 Biological and endogenous factors, Aetiology, Infection, Neurological, Biomarkers, Blood-Brain Barrier, HIV Infections, HIV-1, Humans, Inflammation, Lipopolysaccharides, Neuroinflammatory Diseases, Permeability, cerebrospinal fluid, lipopolysaccharide, HIV-1 infection, neuroinflammation, blood-brain barrier permeability, neuronal injury, Biological Sciences, Medical and Health Sciences, Microbiology, Biological sciences, Biomedical and clinical sciences, Health sciences
Abstract

Human immunodeficiency virus (HIV) infection is associated with increased systemic microbial translocation, neuroinflammation, and occasionally, neuronal injury. Whether systemic lipopolysaccharide (LPS) penetrates into the brain and contributes to neuroinflammation remain unknown in HIV. Here, we measured plasma and cerebrospinal fluid (CSF) LPS levels along with biomarkers of neuroinflammation (white blood cell counts and 40 soluble markers) and neurofilament light chain (NfL). Notably, CSF LPS was undetectable in all samples, including 3 HIV-infected individuals with dementia. Increased plasma LPS, neuroinflammation, and blood-brain barrier (BBB) dysfunction were found in untreated HIV-infected individuals, but not in healthy or treated HIV-infected individuals. Plasma LPS levels were directly correlated with various markers of inflammation in both plasma and CSF, as well as with degree of BBB permeability but not with CSF NfL in HIV-infected subjects. These results suggest that the magnitude of microbial translocation associates with neuroinflammation and BBB permeability in HIV without direct penetration into the central nervous system.

Published
2021

25. SIMPLIFYING THE DISTINCTION BETWEEN LIABILITIES AND EQUITY: The proposed Earned Capital Approach classifies liabilities and equity by distinguishing between a business's external and earned capital

Author

Hill, Mary, Ruch, George W., and Price, Richard A., III

Subjects
Banking, finance and accounting industries, Business, general, Business, Financial Accounting Standards Board -- Powers and duties
Abstract

Developing a clear distinction between liabilities and equity has long been the object of significant amounts of time, energy, and attention in the accounting profession. The Financial Accounting Standards Board [...]

Published
2023

26. Compartmentalization of cerebrospinal fluid inflammation across the spectrum of untreated HIV-1 infection, central nervous system injury and viral suppression

Author

Gisslen, Magnus, Keating, Sheila M, Spudich, Serena, Arechiga, Victor, Stephenson, Sophie, Zetterberg, Henrik, Di Germanio, Clara, Blennow, Kaj, Fuchs, Dietmar, Hagberg, Lars, Norris, Philip J, Peterson, Julia, Shacklett, Barbara L, Yiannoutsos, Constantin T, and Price, Richard W

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, HIV/AIDS, Neurosciences, Clinical Research, Pediatric, Acquired Cognitive Impairment, Pediatric AIDS, Brain Disorders, Infectious Diseases, Evaluation of treatments and therapeutic interventions, 2.1 Biological and endogenous factors, Aetiology, 6.1 Pharmaceuticals, Inflammatory and immune system, Infection, Good Health and Well Being, Adult, Anti-Retroviral Agents, Antiretroviral Therapy, Highly Active, Biomarkers, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Central Nervous System, Cross-Sectional Studies, Female, HIV Infections, HIV-1, Humans, Inflammation, Leukocyte Count, Male, Middle Aged, Neurofilament Proteins, RNA, Viral, Serum Albumin, Sustained Virologic Response, General Science & Technology
Abstract

ObjectiveTo characterize the evolution of central nervous system (CNS) inflammation in HIV-1 infection applying a panel of cerebrospinal fluid (CSF) inflammatory biomarkers to grouped subjects representing a broad spectrum of systemic HIV-1 immune suppression, CNS injury and viral control.MethodsThis is a cross-sectional analysis of archived CSF and blood samples, assessing concentrations of 10 functionally diverse soluble inflammatory biomarkers by immunoassays in 143 HIV-1-infected subjects divided into 8 groups: untreated primary HIV-1 infection (PHI); four untreated groups defined by their blood CD4+ T lymphocyte counts; untreated patients presenting with subacute HIV-associated dementia (HAD); antiretroviral-treated subjects with ≥1 years of plasma viral suppression; and untreated elite controllers. Twenty HIV-1-uninfected controls were included for comparison. Background biomarkers included blood CD4+ and CD8+ T lymphocytes, CSF and blood HIV-1 RNA, CSF white blood cell (WBC) count, CSF/blood albumin ratio, CSF neurofilament light chain (NfL), and CSF t-tau.FindingsHIV-1 infection was associated with a broad compartmentalized CSF inflammatory response that developed early in its course and changed with systemic disease progression, development of neurological injury, and viral suppression. CSF inflammation in untreated individuals without overt HAD exhibited at least two overall patterns of inflammation as blood CD4+ T lymphocytes decreased: one that peaked at 200-350 blood CD4+ T cells/μL and associated with lymphocytic CSF inflammation and HIV-1 RNA concentrations; and a second that steadily increased through the full range of CD4+ T cell decline and associated with macrophage responses and increasing CNS injury. Subacute HAD was distinguished by a third inflammatory profile with increased blood-brain barrier permeability and robust combined lymphocytic and macrophage CSF inflammation. Suppression of CSF and blood HIV-1 infections by antiretroviral treatment and elite viral control were associated with reduced CSF inflammation, though not fully to levels found in HIV-1 seronegative controls.

Published
2021

27. Diffusion Histology Imaging Combining Diffusion Basis Spectrum Imaging (DBSI) and Machine Learning Improves Detection and Classification of Glioblastoma Pathology.

Author

Ye, Zezhong, Price, Richard, Liu, Xiran, Lin, Joshua, Yang, Qingsong, Sun, Peng, Wu, Anthony, Wang, Liang, Han, Rowland, Song, Chunyu, Yang, Ruimeng, Gary, Sam, Mao, Diane, Wallendorf, Michael, Campian, Jian, Li, Jr-Shin, Dahiya, Sonika, Kim, Albert, and Song, Sheng-Kwei

Subjects
Adult, Aged, Algorithms, Diffusion Magnetic Resonance Imaging, Female, Glioblastoma, Humans, Machine Learning, Male, Middle Aged
Abstract

PURPOSE: Glioblastoma (GBM) is one of the deadliest cancers with no cure. While conventional MRI has been widely adopted to examine GBM clinically, accurate neuroimaging assessment of tumor histopathology for improved diagnosis, surgical planning, and treatment evaluation remains an unmet need in the clinical management of GBMs. EXPERIMENTAL DESIGN: We employ a novel diffusion histology imaging (DHI) approach, combining diffusion basis spectrum imaging (DBSI) and machine learning, to detect, differentiate, and quantify areas of high cellularity, tumor necrosis, and tumor infiltration in GBM. RESULTS: Gadolinium-enhanced T1-weighted or hyperintense fluid-attenuated inversion recovery failed to reflect the morphologic complexity underlying tumor in patients with GBM. Contrary to the conventional wisdom that apparent diffusion coefficient (ADC) negatively correlates with increased tumor cellularity, we demonstrate disagreement between ADC and histologically confirmed tumor cellularity in GBM specimens, whereas DBSI-derived restricted isotropic diffusion fraction positively correlated with tumor cellularity in the same specimens. By incorporating DBSI metrics as classifiers for a supervised machine learning algorithm, we accurately predicted high tumor cellularity, tumor necrosis, and tumor infiltration with 87.5%, 89.0%, and 93.4% accuracy, respectively. CONCLUSIONS: Our results suggest that DHI could serve as a favorable alternative to current neuroimaging techniques in guiding biopsy or surgery as well as monitoring therapeutic response in the treatment of GBM.

Published
2020

28. Neurochemical evidence of astrocytic and neuronal injury commonly found in COVID-19

Author

Kanberg, Nelly, Ashton, Nicholas J, Andersson, Lars-Magnus, Yilmaz, Aylin, Lindh, Magnus, Nilsson, Staffan, Price, Richard W, Blennow, Kaj, Zetterberg, Henrik, and Gisslén, Magnus

Subjects
Neurodegenerative, Neurosciences, Clinical Research, Neurological, Good Health and Well Being, Adult, Age Factors, Aged, Astrocytes, Betacoronavirus, Biomarkers, COVID-19, Case-Control Studies, Coronavirus Infections, Female, Follow-Up Studies, Glial Fibrillary Acidic Protein, Humans, Male, Middle Aged, Neurofilament Proteins, Neurons, Pandemics, Pneumonia, Viral, SARS-CoV-2, Severity of Illness Index, Single Molecule Imaging, Clinical Sciences, Cognitive Sciences, Neurology & Neurosurgery
Abstract

ObjectiveTo test the hypothesis that coronavirus disease 2019 (COVID-19) has an impact on the CNS by measuring plasma biomarkers of CNS injury.MethodsWe recruited 47 patients with mild (n = 20), moderate (n = 9), or severe (n = 18) COVID-19 and measured 2 plasma biomarkers of CNS injury by single molecule array, neurofilament light chain protein (NfL; a marker of intra-axonal neuronal injury) and glial fibrillary acidic protein (GFAp; a marker of astrocytic activation/injury), in samples collected at presentation and again in a subset after a mean of 11.4 days. Cross-sectional results were compared with results from 33 age-matched controls derived from an independent cohort.ResultsThe patients with severe COVID-19 had higher plasma concentrations of GFAp (p = 0.001) and NfL (p < 0.001) than controls, while GFAp was also increased in patients with moderate disease (p = 0.03). In patients with severe disease, an early peak in plasma GFAp decreased on follow-up (p < 0.01), while NfL showed a sustained increase from first to last follow-up (p < 0.01), perhaps reflecting a sequence of early astrocytic response and more delayed axonal injury.ConclusionWe show neurochemical evidence of neuronal injury and glial activation in patients with moderate and severe COVID-19. Further studies are needed to clarify the frequency and nature of COVID-19-related CNS damage and its relation to both clinically defined CNS events such as hypoxic and ischemic events and mechanisms more closely linked to systemic severe acute respiratory syndrome coronavirus 2 infection and consequent immune activation, as well as to evaluate the clinical utility of monitoring plasma NfL and GFAp in the management of this group of patients.

Published
2020

29. Cerebrospinal fluid soluble CD30 elevation despite suppressive antiretroviral therapy in individuals living with HIV-1.

Author

Peluso, Michael J, Thanh, Cassandra, Prator, Cecilia A, Hogan, Louise E, Arechiga, Victor M, Stephenson, Sophie, Norris, Philip J, Di Germanio, Clara, Fuchs, Dietmar, Zetterberg, Henrik, Deeks, Steven G, Gisslén, Magnus, Price, Richard W, and Henrich, Timothy J

Subjects
CD30, HIV-1, central nervous system, cerebrospinal fluid, reservoir
Abstract

ObjectivesThe aim of this study was to assess soluble CD30 (sCD30), a protein that colocalises with HIV-1 RNA and DNA in lymphoid cells and tissues, in cerebrospinal fluid (CSF) as a marker of HIV-1 infection in the central nervous system (CNS).MethodsThis was a cross-sectional study using archived samples from two clinical cohorts. Soluble CD30 concentrations were measured in paired CSF and plasma from untreated viraemic individuals (n=52), individuals on suppressive antiretroviral therapy (ART) (n=33), HIV-1 controllers (n=10), participants with CSF HIV-1 'escape' (n=11) and controls without HIV-1 infection (n=16). Nonparametric tests were used to compare levels across groups and evaluate correlations with HIV-1 RNA, CSF neurofilament light chain protein (NFL) and neopterin.ResultsCompared with controls (median 30 ng/mL, interquartile range [IRQ] 23-50), plasma sCD30 levels were elevated in viraemic participants (75 ng/mL, 52-116; P

Published
2020

30. Herpes zoster in HIV-1 infection: The role of CSF pleocytosis in secondary CSF escape and discordance.

Author

Hagberg, Lars, Price, Richard W, Zetterberg, Henrik, Fuchs, Dietmar, and Gisslén, Magnus

Subjects
General Science & Technology
Abstract

HIV cerebrospinal fluid (CSF) escape is defined by a concentration of HIV-1 RNA in CSF above the lower limit of quantification of the employed assay and equal to or greater than the plasma HIV-1 RNA level in the presence of treatment-related plasma viral suppression, while CSF discordance is similarly defined by equal or higher CSF than plasma HIV-1 RNA in untreated individuals. During secondary CSF escape or discordance, disproportionate CSF HIV-1 RNA develops in relation to another infection in addition to HIV-1. We performed a retrospective review of people living with HIV receiving clinical care at Sahlgrenska Infectious Diseases Clinic in Gothenburg, Sweden who developed uncomplicated herpes zoster (HZ) and underwent a research lumbar puncture (LP) within the ensuing 150 days. Based on treatment status and the relationship between CSF and plasma HIV-1 RNA concentrations, they were divided into 4 groups: i) antiretroviral treated with CSF escape (N = 4), ii) treated without CSF escape (N = 5), iii) untreated with CSF discordance (N = 8), and iv) untreated without CSF discordance (N = 8). We augmented these with two additional cases of secondary CSF escape related to neuroborreliosis and HSV-2 encephalitis and analyzed these two non-HZ cases for factors contributing to CSF HIV-1 RNA concentrations. HIV-1 CSF escape and discordance were associated with higher CSF white blood cell (WBC) counts than their non-escape (P = 0.0087) and non-discordant (P = 0.0017) counterparts, and the CSF WBC counts correlated with the CSF HIV-1 RNA levels in both the treated (P = 0.0047) and untreated (P = 0.002) group pairs. Moreover, the CSF WBC counts correlated with the CSF:plasma HIV-1 RNA ratios of the entire group of 27 subjects (P =

Published
2020

31. Exosomal MicroRNAs Associate With Neuropsychological Performance in Individuals With HIV Infection on Antiretroviral Therapy.

Author

OʼMeara, Tess, Kong, Yong, Chiarella, Jennifer, Price, Richard W, Chaudhury, Rabib, Liu, Xinran, Spudich, Serena, Robertson, Kevin, Emu, Brinda, and Lu, Lingeng

Subjects
HIV/AIDS, Neurosciences, Biotechnology, Mental Health, Genetics, Infectious Diseases, Infection, Adult, Anti-HIV Agents, Cross-Sectional Studies, Drug Therapy, Combination, Exosomes, Female, HIV Infections, Humans, Male, MicroRNAs, Middle Aged, Neurocognitive Disorders, Neuropsychological Tests, Signal Transduction, exosomes, HIV-associated neurocognitive dysfunction, microRNA, axon guidance, inflammation, Clinical Sciences, Public Health and Health Services, Virology
Abstract

BackgroundNeurocognitive dysfunction remains prevalent among people living with HIV (PLWH), even after viral suppression on combination antiretroviral therapy (cART). We investigated associations between neuropsychological performance (NP) and patterns of circulating exosomal microRNA (exo-miRNA) expression in PLWH on cART.SettingA cross-sectional examination of plasma exo-miRNA among PLWH on cART with systemic viral suppression and volunteers without HIV infection.MethodsThirty-one PLWH who started cART during early infection (n = 19) or chronic infection (n = 12) participated in phlebotomy and an 11-test neuropsychological battery after >1 year on treatment. NP higher- or lower-performing participants were categorized based on normalized neuropsychological scores. Total RNA was extracted from purified exosomes of 31 PLWH and 5 volunteers without HIV and subject to small RNA sequencing. Differential expression of exo-miRNAs was examined and biological functions were predicted.ResultsEleven exo-miRNAs were up-regulated in NP lower-performing (n = 18) relative to higher-performing PLWH (n = 13). A high proportion of the differentiating exo-miRNA target the axon guidance KEGG pathway and neurotrophin tyrosine receptor kinase signaling Gene Ontology pathway. Differential expression analysis of exo-miRNAs between NP lower- (n = 7) and higher-performing (n = 12) PLWH within the early infection group alone confirmed largely consistent findings.ConclusionsPlasma exo-miRNA content differed between NP higher- and lower-performing PLWH. Several differentially expressed exo-miRNAs were predicted to be involved in inflammation and neurodegeneration pathways. Exo-miRNA in plasma may indicate cross-talk between the circulation and central nervous system and thus may be clinically relevant for neurocognitive dysfunction in PLWH.

Published
2019

32. Bookshelf 2021

Author

Price, Richard and Price, Sally

Published
2022

34. Human Immunodeficiency Virus Type 1 RNA Detected in the Central Nervous System (CNS) After Years of Suppressive Antiretroviral Therapy Can Originate from a Replicating CNS Reservoir or Clonally Expanded Cells.

Author

Joseph, Sarah B, Kincer, Laura P, Bowman, Natalie M, Evans, Chris, Vinikoor, Michael J, Lippincott, Christopher K, Gisslén, Magnus, Spudich, Serena, Menezes, Prema, Robertson, Kevin, Archin, Nancie, Kashuba, Angela, Eron, Joseph J, Price, Richard W, and Swanstrom, Ronald

Subjects
Mental Health, HIV/AIDS, Neurosciences, Infectious Diseases, Aetiology, 2.2 Factors relating to the physical environment, Infection, Adult, Anti-Retroviral Agents, Asymptomatic Diseases, Central Nervous System, Cerebrospinal Fluid, Cohort Studies, Cross-Sectional Studies, Drug Resistance, Viral, Female, HIV Infections, HIV-1, Humans, Longitudinal Studies, Male, Middle Aged, Plasma, RNA, Viral, T-Lymphocytes, Viral Load, CNS, CSF escape, HIV reservoirs, drug resistance, persistence, Biological Sciences, Medical and Health Sciences, Microbiology
Abstract

BACKGROUND:Human immunodeficiency virus type 1 (HIV-1) populations are detected in cerebrospinal fluid (CSF) of some people on suppressive antiretroviral therapy (ART). Detailed analysis of these populations may reveal whether they are produced by central nervous system (CNS) reservoirs. METHODS:We performed a study of 101 asymptomatic participants on stable ART. HIV-1 RNA concentrations were cross-sectionally measured in CSF and plasma. In participants with CSF HIV-1 RNA concentrations sufficient for analysis, viral populations were genetically and phenotypically characterized over multiple time points. RESULTS:For 6% of participants (6 of 101), the concentration of HIV-1 RNA in their CSF was ≥0.5 log copies/mL above that of plasma (ie, CSF escape). We generated viral envelope sequences from CSF of 3 participants. One had a persistent CSF escape population that was macrophage-tropic, partially drug resistant, genetically diverse, and closely related to a minor macrophage-tropic lineage present in the blood prior to viral suppression and enriched for after ART. Two participants (1 suppressed and 1 not) had transient CSF escape populations that were R5 T cell-tropic with little genetic diversity. CONCLUSIONS:Extensive analysis of viral populations in 1 participant revealed that CSF escape was from a persistently replicating population, likely in macrophages/microglia, present in the CNS over 3 years of ART. CSF escape in 2 other participants was likely produced by trafficking and transient expansion of infected T cells in the CNS. Our results show that CNS reservoirs can persist during ART and that CSF escape is not exclusively produced by replicating CNS reservoirs.

Published
2019

35. Human Immunodeficiency Virus Type 1 and Tuberculosis Coinfection in Multinational, Resource-limited Settings: Increased Neurological Dysfunction

Author

Robertson, Kevin R, Oladeji, Bibilola, Jiang, Hongyu, Kumwenda, Johnstone, Supparatpinyo, Khuanchai, Campbell, Thomas B, Hakim, James, Tripathy, Srikanth, Hosseinipour, Mina C, Marra, Christina M, Kumarasamy, Nagalingeswaran, Evans, Scott, Vecchio, Alyssa, La Rosa, Alberto, Santos, Breno, Silva, Marcus T, Montano, Sylvia, Kanyama, Cecilia, Firnhaber, Cindy, Price, Richard, Marcus, Cheryl, Berzins, Baida, Masih, Reena, Lalloo, Umesh, Sanne, Ian, Yosief, Sarah, Walawander, Ann, Nair, Aspara, Sacktor, Ned, Hall, Colin, and Group, 5199 Study Team and the AIDS Clinical Trials

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Neurosciences, Infectious Diseases, Lung, Tuberculosis, Emerging Infectious Diseases, Rare Diseases, HIV/AIDS, Clinical Research, Infection, Good Health and Well Being, Adult, Cognitive Dysfunction, Coinfection, Female, HIV Infections, HIV-1, Health Resources, Humans, Internationality, Longitudinal Studies, Male, Motor Skills, Nervous System Diseases, Neuropsychological Tests, Prospective Studies, Quality of Life, HIV, tuberculosis, resource-limited, cognitive impairment, neuropsychological functioning, Study Team, and the AIDS Clinical Trials Group, Biological Sciences, Medical and Health Sciences, Microbiology, Clinical sciences
Abstract

BackgroundAIDS Clinical Trial Group 5199 compared neurological and neuropsychological test performance of human immunodeficiency virus type 1 (HIV-1)-infected participants in resource-limited settings treated with 3 World Health Organization-recommended antiretroviral (ART) regimens. We investigated the impact of tuberculosis (TB) on neurological and neuropsychological outcomes.MethodsStandardized neurological and neuropsychological examinations were administered every 24 weeks. Generalized estimating equation models assessed the association between TB and neurological/neuropsychological performance.ResultsCharacteristics of the 860 participants at baseline were as follows: 53% female, 49% African; median age, 34 years; CD4 count, 173 cells/μL; and plasma HIV-1 RNA, 5.0 log copies/mL. At baseline, there were 36 cases of pulmonary, 9 cases of extrapulmonary, and 1 case of central nervous system (CNS) TB. Over the 192 weeks of follow-up, there were 55 observations of pulmonary TB in 52 persons, 26 observations of extrapulmonary TB in 25 persons, and 3 observations of CNS TB in 2 persons. Prevalence of TB decreased with ART initiation and follow-up. Those with TB coinfection had significantly poorer performance on grooved pegboard (P < .001) and fingertapping nondominant hand (P < .01). TB was associated with diffuse CNS disease (P < .05). Furthermore, those with TB had 9.27 times (P < .001) higher odds of reporting decreased quality of life, and had 8.02 times (P = .0005) higher odds of loss of productivity.ConclusionsTB coinfection was associated with poorer neuropsychological functioning, particularly the fine motor skills, and had a substantial impact on functional ability and quality of life.Clinical trials registrationNCT00096824.

Published
2019

36. Predicting Efavirenz Concentrations in the Brain Tissue of HIV‐Infected Individuals and Exploring their Relationship to Neurocognitive Impairment

Author

Srinivas, Nithya, Joseph, Sarah Beth, Robertson, Kevin, Kincer, Laura P, Menezes, Prema, Adamson, Lourdes, Schauer, Amanda P, Blake, Kimberly H, White, Nicole, Sykes, Craig, Luciw, Paul, Eron, Joseph J, Forrest, Alan, Price, Richard W, Spudich, Serena, Swanstrom, Ronald, and Kashuba, Angela DM

Subjects
Pharmacology and Pharmaceutical Sciences, Biomedical and Clinical Sciences, Cardiovascular Medicine and Haematology, Brain Disorders, Acquired Cognitive Impairment, Neurosciences, Mental Health, HIV/AIDS, Pediatric AIDS, Pediatric, Infectious Diseases, Infection, Adult, Alkynes, Animals, Benzoxazines, Brain, Cognition Disorders, Cyclopropanes, Female, HIV Infections, Humans, Macaca mulatta, Male, Middle Aged, Young Adult, Cardiorespiratory Medicine and Haematology, Oncology and Carcinogenesis, Other Medical and Health Sciences, General Clinical Medicine, Cardiovascular medicine and haematology, Pharmacology and pharmaceutical sciences
Abstract

Sparse data exist on the penetration of antiretrovirals into brain tissue. In this work, we present a framework to use efavirenz (EFV) pharmacokinetic (PK) data in plasma, cerebrospinal fluid (CSF), and brain tissue of eight rhesus macaques to predict brain tissue concentrations in HIV-infected individuals. We then perform exposure-response analysis with the model-predicted EFV area under the concentration-time curve (AUC) and neurocognitive scores collected from a group of 24 HIV-infected participants. Adult rhesus macaques were dosed daily with 200 mg EFV (as part of a four-drug regimen) for 10 days. Plasma was collected at 8 time points over 10 days and at necropsy, whereas CSF and brain tissue were collected at necropsy. In the clinical study, data were obtained from one paired plasma and CSF sample of participants prescribed EFV, and neuropsychological test evaluations were administered across 15 domains. PK modeling was performed using ADAPT version 5.0 Biomedical Simulation Resource, Los Angeles, CA) with the iterative two-stage estimation method. An eight-compartment model best described EFV distribution across the plasma, CSF, and brain tissue of rhesus macaques and humans. Model-predicted median brain tissue concentrations in humans were 31 and 8,000 ng/mL, respectively. Model-predicted brain tissue AUC was highly correlated with plasma AUC (γ = 0.99, P  0.05). This analysis provides an approach to estimate PK the brain tissue in order to perform PK/pharmacodynamic analyses at the target site.

Published
2019

37. Elevated cerebrospinal fluid Galectin-9 is associated with central nervous system immune activation and poor cognitive performance in older HIV-infected individuals

Author

Premeaux, Thomas A, D’Antoni, Michelle L, Abdel-Mohsen, Mohamed, Pillai, Satish K, Kallianpur, Kalpana J, Nakamoto, Beau K, Agsalda-Garcia, Melissa, Shiramizu, Bruce, Shikuma, Cecilia M, Gisslén, Magnus, Price, Richard W, Valcour, Victor, and Ndhlovu, Lishomwa C

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Pediatric AIDS, Neurosciences, Aging, Pediatric, HIV/AIDS, Clinical Research, Brain Disorders, Infectious Diseases, Acquired Cognitive Impairment, Aetiology, 2.1 Biological and endogenous factors, Neurological, Infection, Adult, Age Factors, Anti-HIV Agents, Antigens, CD, Antigens, Differentiation, Myelomonocytic, Antiretroviral Therapy, Highly Active, Biomarkers, Case-Control Studies, Central Nervous System, Cognition, Female, Galectins, HIV Infections, HIV-1, Humans, Male, Middle Aged, Neopterin, Neuropsychological Tests, RNA, Viral, Receptors, Cell Surface, Viremia, Galectin-9, HIV, Neuroinflammation, Cognitive disorders, Clinical Sciences, Virology, Clinical sciences, Medical microbiology
Abstract

We previously reported that galectin-9 (Gal-9), a soluble lectin with immunomodulatory properties, is elevated in plasma during HIV infection and induces HIV transcription. The link between Gal-9 and compromised neuronal function is becoming increasingly evident; however, the association with neuroHIV remains unknown. We measured Gal-9 levels by ELISA in cerebrospinal fluid (CSF) and plasma of 70 HIV-infected (HIV+) adults stratified by age (older > 40 years and younger

Published
2019

38. Cerebrospinal Fluid Concentrations of the Synaptic Marker Neurogranin in Neuro-HIV and Other Neurological Disorders.

Author

Yilmaz, Aylin, Fuchs, Dietmar, Price, Richard W, Spudich, Serena, Blennow, Kaj, Zetterberg, Henrik, and Gisslén, Magnus

Subjects
Frontal Lobe, Humans, HIV Infections, AIDS Dementia Complex, Lewy Body Disease, Parkinson Disease, Alzheimer Disease, Interleukin-8, Adult, Middle Aged, Female, Male, Neurogranin, Interleukin-1beta, Frontotemporal Dementia, Biomarkers, Cerebrospinal fluid, HIV, Virology, Immunology, Medical Microbiology
Abstract

Purpose of reviewThe aim of this study was to examine the synaptic biomarker neurogranin in cerebrospinal fluid (CSF) in different stages of HIV infection and in relation to what is known about CSF neurogranin in other neurodegenerative diseases.Recent findingsCSF concentrations of neurogranin are increased in Alzheimer's disease, but not in other neurodegenerative disorder such as Parkinson's disease, frontotemporal dementia, and Lewy body dementia. Adults with HIV-associated dementia have been found to have decreased levels of neurogranin in the frontal cortex, which at least to some extent, may be mediated by the proinflammatory cytokines IL-1β and IL-8. CSF neurogranin concentrations were in the same range for all groups of HIV-infected individuals and uninfected controls. This either indicates that synaptic injury is not an important part of HIV neuropathogenesis or that CSF neurogranin is not sensitive to the type of synaptic impairment present in HIV-associated neurocognitive disorders.

Published
2019

39. CSF concentrations of soluble TREM2 as a marker of microglial activation in HIV-1 infection

Author

Gisslén, Magnus, Heslegrave, Amanda, Veleva, Elena, Yilmaz, Aylin, Andersson, Lars-Magnus, Hagberg, Lars, Spudich, Serena, Fuchs, Dietmar, Price, Richard W, and Zetterberg, Henrik

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Clinical Sciences, Immunology, Infectious Diseases, Acquired Cognitive Impairment, Neurosciences, Brain Disorders, Clinical Research, HIV/AIDS, 6.1 Pharmaceuticals, Evaluation of treatments and therapeutic interventions, 2.1 Biological and endogenous factors, Aetiology, Infection, Good Health and Well Being, AIDS Dementia Complex, Adult, Aged, Biomarkers, Cross-Sectional Studies, Female, HIV Infections, HIV-1, Humans, Macrophage Activation, Male, Membrane Glycoproteins, Microglia, Middle Aged, Receptors, Immunologic, Retrospective Studies, Young Adult
Abstract

ObjectiveTo explore changes in CSF sTREM2 concentrations in the evolving course of HIV-1 infection.MethodsIn this retrospective cross-sectional study, we measured concentrations of the macrophage/microglial activation marker sTREM2 in CSF samples from 121 HIV-1-infected adults and 11 HIV-negative controls and examined their correlations with other CSF and blood biomarkers of infection, inflammation, and neuronal injury.ResultsCSF sTREM2 increased with systemic and CNS HIV-1 disease severity, with the highest levels found in patients with HIV-associated dementia (HAD). In untreated HIV-1-infected patients without an HAD diagnosis, levels of CSF sTREM2 increased with decreasing CD4+ T-cell counts. CSF concentrations of both sTREM2 and the neuronal injury marker neurofilament light protein (NFL) were significantly associated with age. CSF sTREM2 levels were also independently correlated with CSF NFL. Notably, this association was also observed in HIV-negative controls with normal CSF NFL. HIV-infected patients on suppressive antiretroviral treatment had CSF sTREM2 levels comparable to healthy controls.ConclusionsElevations in CSF sTREM2 levels, an indicator of macrophage/microglial activation, are a common feature of untreated HIV-1 infection that increases with CD4+ T-cell loss and reaches highest levels in HAD. The strong and independent association between CSF sTREM2 and CSF NFL suggests a linkage between microglial activation and neuronal injury in HIV-1 infection. CSF sTREM2 has the potential of being a useful biomarker of innate CNS immune activation in different stages of untreated and treated HIV-1 infection.

Published
2019

40. Plasma concentration of neurofilament light chain protein decreases after switching from tenofovir disoproxil fumarate to tenofovir alafenamide fumarate

Author

Hermansson, Linn, Yilmaz, Aylin, Price, Richard W, Nilsson, Staffan, McCallister, Scott, Makadzange, Tariro, Das, Moupali, Zetterberg, Henrik, Blennow, Kaj, and Gisslen, Magnus

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Clinical Trials and Supportive Activities, Neurosciences, Clinical Research, Adenine, Adult, Alanine, Anti-HIV Agents, Central Nervous System Diseases, Creatinine, Drug Administration Schedule, Female, HIV Infections, Humans, Male, Middle Aged, Neurofilament Proteins, Tenofovir, General Science & Technology
Abstract

BackgroundBecause tenofovir alafenamide (TAF) leads to significantly lower plasma tenofovir concentrations than tenofovir disoproxil fumarate (TDF) and is a stronger substrate for P-glycoprotein (P-gp) than TDF, TAF could lead to decreased central nervous system (CNS) tenofovir exposure than TDF. We aimed to determine if switching from TDF to TAF increases the risk of neuronal injury, by quantifying plasma levels of neurofilament light protein (NfL), a sensitive marker of neuronal injury in HIV CNS infection.MethodsPlasma NfL concentration was measured at baseline, week 24, and week 84 in stored plasma samples from 416 participants (272 switching to elvitegravir (E)/cobicistat (C)/emtricitabine (F)/TAF and 144 continuing E/C/F/TDF) enrolled in the randomized, active-controlled, multicenter, open-label, noninferiority Gilead GS-US-292-0109 trial.ResultsWhile plasma NfL levels in both groups were within the normal range, we found a small but significant decrease in the E/C/F/TAF arm after 84 weeks from a geometric mean of 9.3 to 8.8 pg/mL (5.4% decline, 95% CI 2.0-8.4, p = 0.002). This change was significantly different (p = 0.001) from that of the E/C/F/TDF arm, in which plasma NfL concentration changed from 9.7 pg/mL at baseline to 10.2 pg/mL at week 84 (5.8% increase, 95% CI -0.8-12.9, p = 0.085). This increase is in line with what could be expected in normal ageing. Plasma NfL concentrations significantly correlated with age. No correlation was found between plasma NfL and serum creatinine.ConclusionsWe found no biomarker evidence of CNS injury when switching from TDF to TAF. It is unclear whether the small decrease in plasma NfL found after switch to TAF is of any clinical relevance, particularly with plasma NfL levels in both arms remaining within the limits found in HIV-negative controls. These results indicate that switching from TDF to TAF appears safe with regard to neuronal injury.

Published
2019

41. Longitudinal Trajectories of Brain Volume and Cortical Thickness in Treated and Untreated Primary Human Immunodeficiency Virus Infection

Author

Sanford, Ryan, Ances, Beau M, Meyerhoff, Dieter J, Price, Richard W, Fuchs, Dietmar, Zetterberg, Henrik, Spudich, Serena, and Collins, D Louis

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Neurosciences, Pediatric, Behavioral and Social Science, Infectious Diseases, HIV/AIDS, Clinical Research, Sexually Transmitted Infections, Biomedical Imaging, Brain Disorders, Mental Health, 2.1 Biological and endogenous factors, Aetiology, Neurological, Infection, Adult, Anti-Retroviral Agents, Biomarkers, Brain, Cerebral Cortex, Cross-Sectional Studies, Drug Therapy, Combination, Female, HIV Infections, Humans, Longitudinal Studies, Magnetic Resonance Imaging, Male, Middle Aged, Neuroimaging, primary HIV infection, brain morphometry, combination antiretroviral therapy, magnetic resonance imaging, HIV-associated neurocognitive disorders, Biological Sciences, Medical and Health Sciences, Microbiology, Clinical sciences
Abstract

BackgroundHuman immunodeficiency virus (HIV) penetrates the brain in early infection. We used neuroimaging to longitudinally examine the impact of HIV and combination antiretroviral therapy (cART) on the brain in treated and untreated HIV-infected participants, starting in primary HIV infection (PHI).MethodsSixty-five participants, enrolled during PHI, underwent longitudinal magnetic resonance imaging, 30 of whom commenced cART during follow-up. Cross-sectional data from 16 patients with chronic HIV infection (CHI) and 19 HIV-uninfected participants were included for comparison. Brain volume and cortical thickness were estimated using tensor-based morphometry and cortical modeling, respectively. Mixed-effects models longitudinally mapped structural brain changes before and after cART. The relationship between brain morphometry estimates and blood and cerebrospinal fluid (CSF) biomarkers were also tested. Region-of-interest analyses were performed to compare brain morphometry estimates between the groups.ResultsPrior to cART, longer duration of untreated infection in PHI correlated with volume loss in the thalamus, caudate, and cerebellum, and with cortical thinning in the frontal and temporal lobes and cingulate cortex. After cART, no further volume loss was observed. However, small increases of cortical thickness in the frontal and temporal lobe correlated with longer cART duration. No correlations were observed with blood or CSF measures. The PHI group did not have different brain morphometric measures compared to the HIV-uninfected group, but had larger volumes in the thalamus, caudate, putamen, and cortical gray matter compared with CHI participants.ConclusionsSubcortical atrophy and cortical thinning occur during untreated infection but may be arrested by cART. These findings emphasize the importance of early cART.

Published
2018

43. Closed Timelike Curves and 'Effective' Superluminal Travel with Naked Line Singularities

Author

Mallary, Caroline, Khanna, Gaurav, and Price, Richard

Subjects
General Relativity and Quantum Cosmology
Abstract

We examine closed timelike curves (CTCs) and "effective" superluminal travel in a spacetime containing naked line singularities, which we call "wires". Each wire may be straight-line singularity or a ring singularity. The Weak Energy Condition (WEC) is preserved in all well-defined regions of the spacetime. (The singularities themselves are not well-defined, so the WEC is undefined there, but it is never explicitly violated.) Parallel to the wire, "effective" superluminal travel is possible, in that the wire may be used as a shortcut between distant regions of spacetime. Our purpose in presenting the superluminal aspects of the wire is to dispel the commonly held view that explicit WEC violation is necessary for effective superluminal travel, whereas in truth the strictures against superluminal travel are more complicated. We also demonstrate how the existence of such "wires" could create CTCs. We present a model spacetime which contains two wires which are free to move relative to each other. This spacetime is asymptotically flat: It becomes a Minkowski spacetime a finite distance away from each of the wires. The CTCs under investigation do not need to enter the wires' singularities, and can be confined to regions that are weak-field: This means that if these wires were physically possible, they would present causality problems even in nonsingular, energetically realistic regions of the spacetime. We conclude that the Weak Energy Condition alone is not sufficient to prevent superluminal travel in asymptotically flat spacetimes., Comment: 10 pages, 5 figures; animation available on YouTube at: https://youtu.be/ub6PGaygVwA

Published
2017
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44. Scalar Fields in Black Hole Spacetimes

Author

Thuestad, Izak, Khanna, Gaurav, and Price, Richard

Subjects
General Relativity and Quantum Cosmology
Abstract

The time-evolution of matter fields in black hole exterior spacetimes is a well-studied subject, spanning several decades of research. However, the behavior of fields in the black hole interior spacetime, has only relatively recently begun receiving some attention from the research community. In this paper, we numerically study the late-time evolution of scalar fields in both Schwarzschild and Kerr spacetimes, including the black hole interior. We recover the expected late-time power-law "tails" on the exterior (null infinity, time-like infinity and the horizon). In the interior region, we find an interesting oscillatory behavior that is characterized by the multipole index $\ell$ of the scalar field. In addition, we also study the extremal Kerr case and find strong indications of an instability developing at the horizon., Comment: 9 pages, 10 figures; updated references

Published
2017
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45. Gravitational wave sources: reflections and echoes

Author

Price, Richard and Khanna, Gaurav

Subjects
General Relativity and Quantum Cosmology, Astrophysics - High Energy Astrophysical Phenomena
Abstract

The recent detection of gravitational waves has generated interest in alternatives to the black hole interpretation of sources. One set of such alternatives involves a prediction of gravitational wave "echoes". We consider two aspects of possible echoes: First, general features of echoes coming from spacetime reflecting conditions. We find that the detailed nature of such echoes does not bear any clear relationship to quasi-normal frequencies. Second, we point out the pitfalls in the analysis of local reflecting "walls" near the horizon of rapidly rotating black holes., Comment: 8 pages, 4 figures; to appear in Classical & Quantum Gravity (CQG)

Published
2017
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46. Changes in cerebrospinal fluid proteins across the spectrum of untreated and treated chronic HIV-1 infection.

Author

Hu, Zicheng, Cinque, Paola, Dravid, Ameet, Hagberg, Lars, Yilmaz, Aylin, Zetterberg, Henrik, Fuchs, Dietmar, Gostner, Johanna, Blennow, Kaj, Spudich, Serena S., Kincer, Laura, Zhou, Shuntai, Joseph, Sarah Beth, Swanstrom, Ronald, Price, Richard W., and Gisslén, Magnus

Subjects
CENTRAL nervous system injuries, CEREBROSPINAL fluid, ANTIRETROVIRAL agents, HIV, CEREBROSPINAL fluid examination, CYTOPLASMIC filaments
Abstract

Using the Olink Explore 1536 platform, we measured 1,463 unique proteins in 303 cerebrospinal fluid (CSF) specimens from four clinical centers contributed by uninfected controls and 12 groups of people living with HIV-1 infection representing the spectrum of progressive untreated and treated chronic infection. We present three initial analyses of these measurements: an overview of the CSF protein features of the sample; correlations of the CSF proteins with CSF HIV-1 RNA and neurofilament light chain protein (NfL) concentrations; and comparison of CSF proteins in HIV-associated dementia (HAD) and neurosymptomatic CSF escape (NSE). These reveal a complex but coherent picture of CSF protein changes with highest concentrations of many proteins during CNS injury in the HAD and NSE groups and variable protein changes across the course of systemic HIV-1 progression that included two common patterns, designated as lymphoid and myeloid patterns, related to principal involvement of their underlying inflammatory cell lineages. Antiretroviral therapy reduced CSF protein perturbations, though not always to control levels. The dataset of these CSF protein measurements, along with background clinical information, is posted online. Extended studies of this unique dataset will supplement this report to provide more detailed characterization of the dynamic impact of HIV-1 infection on the CSF proteome across the spectrum of HIV-1 infection, advancing the mechanistic understanding of HIV-1-related CNS pathobiology. Author summary: We measured more than 1,400 proteins in 303 cerebrospinal fluid (CSF) specimens from a representative broad range of untreated and treated people living with HIV-1 infection (PLWH) along with uninfected controls who volunteered for our studies. The results defined a complex, but generally coherent set of changes in many immune-inflammatory proteins and several central nervous system injury biomarkers as systemic HIV-1 infection progressed. There was a marked increase in many of these protein biomarkers in individuals with HIV-associated dementia and neurosymptomatic CSF viral escape, two conditions distinguished by overt, and characteristically severe, neurological impairment. The resultant data and initial analyses advance the characterization and understanding of the evolution of HIV-driven neuropathogenesis. The large dataset from this study is posted online and available to other investigators for further analyses, extending the utility of these data and potentially aiding in developing future prevention, diagnosis and mitigation of the deleterious impact of HIV-1 infection on the brain. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
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47. Tissue- and Temporal-Dependent Dynamics of Myeloablation in Response to Gemcitabine Chemotherapy.

Author

Kitelinger, Lydia E., Thim, Eric A., Zipkowitz, Sarah Y., Price, Richard J., and Bullock, Timothy N. J.

Subjects
MYELOID-derived suppressor cells, PROGNOSIS, TRIPLE-negative breast cancer, TUMOR microenvironment, CANCER invasiveness
Abstract

For triple-negative breast cancer (TNBC), the most aggressive subset of breast cancer, immune cell infiltrates have prognostic implications. The presence of myeloid-derived suppressor cells supports tumor progression, while tumor-infiltrating lymphocytes (TILs) correlate with improved survival and responsiveness to immunotherapy. Manipulating the abundance of these populations may enhance tumor immunity. Gemcitabine (GEM), a clinically employed chemotherapeutic, is reported to be systemically myeloablative, and thus it is a potentially useful adjunct therapy for promoting anti-tumor immunity. However, knowledge about the immunological effects of GEM intratumorally is limited. Thus, we directly compared the impact of systemic GEM on immune cell presence and functionality in the tumor microenvironment (TME) to its effects in the periphery. We found that GEM is not myeloablative in the TME; rather, we observed sustained, significant reductions in TILs and dendritic cells—crucial components in initiating an adaptive immune response. We also performed bulk-RNA sequencing to identify immunological alterations transcriptionally induced by GEM. While we found evidence of upregulation in the interferon-gamma (IFN-γ) response pathway, we determined that GEM-mediated growth control is not dependent on IFN-γ. Overall, our findings yield new insights into the tissue- and temporal-dependent immune ablative effects of GEM, contrasting the paradigm that this therapy is specifically myeloablative. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
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48. Bookshelf 2020

Author

Price, Richard and Price, Sally

Published
2021

49. Large Differences in Small RNA Composition Between Human Biofluids.

Author

Godoy, Paula M, Bhakta, Nirav R, Barczak, Andrea J, Cakmak, Hakan, Fisher, Susan, MacKenzie, Tippi C, Patel, Tushar, Price, Richard W, Smith, James F, Woodruff, Prescott G, and Erle, David J

Subjects
Body Fluids, Humans, Amino Acids, MicroRNAs, RNA, Small Interfering, RNA, Transfer, Anticodon, Sequence Analysis, RNA, Adult, Middle Aged, Female, Male, Y RNA, biofluids, extracellular RNA, miRNA, tRNA, Biotechnology, Genetics, Biochemistry and Cell Biology, Medical Physiology
Abstract

Extracellular microRNAs (miRNAs) and other small RNAs are implicated in cellular communication and may be useful as disease biomarkers. We systematically compared small RNAs in 12 human biofluid types using RNA sequencing (RNA-seq). miRNAs and tRNA-derived RNAs (tDRs) accounted for the majority of mapped reads in all biofluids, but the ratio of miRNA to tDR reads varied from 72 in plasma to 0.004 in bile. miRNA levels were highly correlated across all biofluids, but levels of some miRNAs differed markedly between biofluids. tDR populations differed extensively between biofluids. Y RNA fragments were seen in all biofluids and accounted for >10% of reads in blood plasma, serum, and cerebrospinal fluid (CSF). Reads mapping exclusively to Piwi-interacting RNAs (piRNAs) were very rare, except in seminal plasma. These results demonstrate extensive differences in small RNAs between human biofluids and provide a useful resource for investigating extracellular RNA biology and developing biomarkers.

Published
2018

50. Single-cell RNA sequencing reveals microglia-like cells in cerebrospinal fluid during virologically suppressed HIV

Author

Farhadian, Shelli F, Mehta, Sameet S, Zografou, Chrysoula, Robertson, Kevin, Price, Richard W, Pappalardo, Jenna, Chiarella, Jennifer, Hafler, David A, and Spudich, Serena S

Subjects
Infectious Diseases, Neurodegenerative, HIV/AIDS, Neurosciences, Pediatric AIDS, Genetics, Pediatric, Aetiology, 2.1 Biological and endogenous factors, Infection, Neurological, Good Health and Well Being, Adult, Anti-HIV Agents, Antiretroviral Therapy, Highly Active, Blood, Central Nervous System, Cerebrospinal Fluid, Gene Expression, HIV Infections, Humans, Inflammation, Microglia, Myeloid Cells, Neurodegenerative Diseases, Sequence Analysis, RNA, AIDS/HIV, Neurological disorders
Abstract

Central nervous system (CNS) immune activation is an important driver of neuronal injury during several neurodegenerative and neuroinflammatory diseases. During HIV infection, CNS immune activation is associated with high rates of neurocognitive impairment, even during sustained long-term suppressive antiretroviral therapy (ART). However, the cellular subsets that drive immune activation and neuronal damage in the CNS during HIV infection and other neurological conditions remain unknown, in part because CNS cells are difficult to access in living humans. Using single-cell RNA sequencing (scRNA-seq) on cerebrospinal fluid (CSF) and blood from adults with and without HIV, we identified a rare (

Published
2018

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