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2. Report of the European DNA profiling group (EDNAP) - an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656.

Author

Gill, Peter, D'Aloja, E., Dupuy, B., Eriksen, B., Jangblad, M., Johnsson, V., Kloosterman, A.D., Kratzer, A., Lareu, Maria-Victoria, Mevag, B., Morling, N., Phillips, C., Pfitzinger, H., Rand, S., Sabatier, M., Scheithauer, R., Schmitter, H., Schneider, Peter M., Skitsa, I., and Vide, M. Conceicao

Subjects
DNA testing -- Research
Published
1998

5. Report on the third EDNAP collaborative STR exercise.

Author

Andersen, Julia F., Martin, Peter, Carracedo, A., Dobosz, M., Eriksen, B., Johnsson, V., Kimpton, C.P., Kloosterman, A., Konialis, C., Kratzer, A., Phillips, P., Mevag, B., Pfitzinger, H., Rand, S., Rosen, B., Schmitter, H., Schneider, P., and Vide, M.

Subjects
DNA testing -- International aspects, Criminal investigation -- International aspects, Evidence, Criminal -- International aspects
Published
1996

6. Report of the European DNA Profiling Group (EDNAP)--an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656

Author

Gill, P, d'Aloja, E, Dupuy, B, Eriksen, Birthe, Jangblad, M, Johnsson, V, Kloosterman, A D, Kratzer, A, Lareu, M V, Mevag, B, Morling, N, Phillips, C, Pfitzinger, H, Rand, S, Sabatier, M, Scheithauer, R, Schmitter, H, Schneider, Peter M., Skitsa, I, Vide, M C, Gill, P, d'Aloja, E, Dupuy, B, Eriksen, Birthe, Jangblad, M, Johnsson, V, Kloosterman, A D, Kratzer, A, Lareu, M V, Mevag, B, Morling, N, Phillips, C, Pfitzinger, H, Rand, S, Sabatier, M, Scheithauer, R, Schmitter, H, Schneider, Peter M., Skitsa, I, and Vide, M C

Abstract

Udgivelsesdato: 1998-Dec-21, This paper describes the results of three collaborative exercises which continues the EDNAP theme to explore whether uniformity of DNA profiling results could be achieved between European laboratories using STRs. In an earlier exercise, complex hypervariable AAAG-repeat STR loci were investigated, but reproducibility was found to be poor because of the variation of techniques used by participating laboratories. In the exercise reported here, an internal allelic ladder composed of ACTBP2 and D11S554 fragments was distributed. This ladder was used to size ACTBP2 analysed by a "singleplex" PCR amplification and D11S554 combined with APOAI1 in a separate "duplex" reaction. Laboratories were asked to test 7 blood stains, one of which was a known control, and to report the results to the co-ordinating laboratory. The exercise demonstrated that ACTBP2 showed good reproducibility between laboratories, whereas further testing would be needed to validate APOAI1 and D11S554 for interlaboratory comparisons. In separate exercises, the simple loci D12S391 and D1S1656 were tested; both of these showed excellent reproducibility between laboratories.

Published
1998

7. Report of the European DNA profiling group (EDNAP): an investigation of the complex STR loci D21S11 and HUMFIBRA (FGA)

Author

Gill, Peter, primary, d'Aloja, E, additional, Andersen, J, additional, Dupuy, B, additional, Jangblad, M, additional, Johnsson, V, additional, Kloosterman, A.D, additional, Kratzer, A, additional, Lareu, M.V, additional, Meldegaard, M, additional, Phillips, C, additional, Pfitzinger, H, additional, Rand, S, additional, Sabatier, M, additional, Scheithauer, R, additional, Schmitter, H, additional, Schneider, P, additional, and Vide, M.C, additional

Published
1997
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9. Report on the second EDNAP collaborative STR exercise

Author

Kimpton, Colin, primary, Gill, Peter, additional, D'Aloja, E., additional, Andersen, J.F., additional, Bar, W., additional, Holgersson, S., additional, Jacobsen, S., additional, Johnsson, V., additional, Kloosterman, A.D., additional, Lareu, M.V., additional, Nellemann, L., additional, Pfitzinger, H., additional, Phillips, C.P., additional, Rand, S., additional, Schmitter, H., additional, Schneider, P.M., additional, Sternersen, M., additional, and Vide, M.C., additional

Published
1995
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10. Report of the European DNA profiling group (EDNAP) — towards standardisation of short tandem repeat (STR) loci

Author

Gill, P., primary, Kimpton, C., additional, D'Aloja, E., additional, Andersen, J.F., additional, Bar, W., additional, Brinkmann, B., additional, Holgersson, S., additional, Johnsson, V., additional, Kloosterman, A.D., additional, Lareu, M.V., additional, Nellemann, L., additional, Pfitzinger, H., additional, Phillips, C.P., additional, Schmitter, H., additional, Schneider, P.M., additional, and Stenersen, M., additional

Published
1994
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11. Results of collaborative study regarding the standardization of the Y-linked STR system DYS385 by the European DNA Profiling (EDNAP) group.

Author

Schneider PM, d'Aloja E, Dupuy BM, Eriksen B, Jangblad A, Kloosterman AD, Kratzer A, Lareu MV, Pfitzinger H, Rand S, Scheithauer R, Schmitter H, Skitsa I, Syndercombe-Court D, and Vide MC

Subjects
Blood Protein Electrophoresis methods, Blood Protein Electrophoresis standards, Europe, Genetics, Population, Humans, Male, Reproducibility of Results, Blood Stains, DNA Fingerprinting methods, DNA Fingerprinting standards, Genetic Linkage genetics, International Cooperation, Minisatellite Repeats genetics, Y Chromosome genetics
Abstract

Y-chromosome linked short tandem repeat (STR) loci are inherited as a closely linked haplotype, which appears to remain stable in a given paternal lineage over many generations. In forensic cases, Y-linked STRs are particularly useful for the identification of human remains as well as in rape cases with mixed male/female stain samples. DYS385 is derived from tandemly duplicated segments of the Y chromosome thus giving rise to two fragments of variable length which do not behave like alleles but genotypes. The European DNA Profiling (EDNAP) group has carried out a collaborative exercise among 14 participating laboratories using DYS385 for typing of five unknown bloodstains and a control sample. Furthermore, population data from eight different European countries with samples sizes between 91 and 150 male individuals were collected. The results confirm previous observations that DYS385 is one of the most informative Y-linked STR loci. It could also be demonstrated that reproducible results can be obtained independently from the electrophoretic separation and detection methods used. Thus DYS385 may serve as a useful complementation to the routinely used autosomal STR systems in special cases.

Published
1999
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12. Adjustment of the tRNA population to the codon usage in chloroplasts.

Author

Pfitzinger H, Guillemaut P, Weil JH, and Pillay DT

Subjects
Fabaceae genetics, Plant Proteins genetics, Plants genetics, Plants, Medicinal, Chloroplasts metabolism, Codon, RNA, Messenger, RNA, Transfer genetics
Abstract

In chloroplasts there is a correlation between the amounts of tRNAs specific for a given amino acid and the codons specifying this amino acid. Furthermore, for the amino acids coded for by more than one codon, the population of isoaccepting tRNAs is adjusted to the frequency of synonymous codons used in chloroplast protein genes. A comparison by two-dimensional gel electrophoresis of the tRNA populations extracted from chloroplasts and from chloroplast polysomes shows that all chloroplast tRNAs are involved in protein biosynthesis.

Published
1987
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