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4 results on '"PDMS microdevice"'

1. PDMS-Based Microdevices for the Capture of MicroRNA Biomarkers.

Author

Lunelli, Lorenzo, Barbaresco, Federica, Scordo, Giorgio, Potrich, Cristina, Vanzetti, Lia, Marasso, Simone Luigi, Cocuzza, Matteo, Pirri, Candido Fabrizio, and Pederzolli, Cecilia

Subjects
MICROFLUIDIC devices, LABS on a chip, NON-small-cell lung carcinoma, MICRORNA, BIOMARKERS
Abstract

The isolation and analysis of circulating biomarkers, the main concern of liquid biopsy, could greatly benefit from microfluidics. Microfluidics has indeed the huge potentiality to bring liquid biopsy into the clinical practice. Here, two polydimethylsiloxane (PDMS)-based microdevices are presented as valid tools for capturing microRNAs biomarkers from clinically-relevant samples. After an extensive study of functionalized polydimethylsiloxane (PDMS) properties in adsorbing/eluting microRNAs, the best conditions were transferred to the microdevices, which were thoroughly characterized. The channels morphology and chemical composition were measured, and parameters for the automation of measures were setup. The best working conditions were then used with microdevices, which were proven to capture microRNAs on all channel surfaces. Finally, microfluidic devices were successfully validated via real-time PCR for the detection of a pool of microRNAs related to non-small cell lung cancer, selected as proof-of-principle. The microfluidic approach described here will allow a step forward towards the realization of an efficient microdevice, possibly automated and integrated into a microfluidic lab-on-a-chip with high analytical potentialities. [ABSTRACT FROM AUTHOR]

Published
2020
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2. PDMS-Based Microdevices for the Capture of MicroRNA Biomarkers

Author

Lorenzo Lunelli, Federica Barbaresco, Giorgio Scordo, Cristina Potrich, Lia Vanzetti, Simone Luigi Marasso, Matteo Cocuzza, Candido Fabrizio Pirri, and Cecilia Pederzolli

Subjects
PDMS microdevice, surface functionalization, microRNAs, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
Abstract

The isolation and analysis of circulating biomarkers, the main concern of liquid biopsy, could greatly benefit from microfluidics. Microfluidics has indeed the huge potentiality to bring liquid biopsy into the clinical practice. Here, two polydimethylsiloxane (PDMS)-based microdevices are presented as valid tools for capturing microRNAs biomarkers from clinically-relevant samples. After an extensive study of functionalized polydimethylsiloxane (PDMS) properties in adsorbing/eluting microRNAs, the best conditions were transferred to the microdevices, which were thoroughly characterized. The channels morphology and chemical composition were measured, and parameters for the automation of measures were setup. The best working conditions were then used with microdevices, which were proven to capture microRNAs on all channel surfaces. Finally, microfluidic devices were successfully validated via real-time PCR for the detection of a pool of microRNAs related to non-small cell lung cancer, selected as proof-of-principle. The microfluidic approach described here will allow a step forward towards the realization of an efficient microdevice, possibly automated and integrated into a microfluidic lab-on-a-chip with high analytical potentialities.

Published
2020
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3. Epoxy resin mold and PDMS microfluidic devices through photopolymer flexographic printing plate

Author

Karla Vizuete, Gustavo Rosero, Carol M. Olmos, Luis Cumbal, Ana Peñaherrera, Maximiliano S. Pérez, Alexis Debut, Betiana Lerner, Igor de Sá Carneiro, Carlos R. Arroyo, Camilo Perez, and Andrea V. Vaca

Subjects
Fabrication, Materials science, Scanning electron microscope, PHOTOPOLYMER FLEXOGRAPHIC, Microfluidics, Nanotechnology, 02 engineering and technology, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, chemistry.chemical_compound, Mold, Flexography, Materials Chemistry, medicine, PDMS MICRODEVICE, Electrical and Electronic Engineering, Instrumentation, MICRODROPLETS GENERATION, MICROFLUIDICS, Polydimethylsiloxane, Metals and Alloys, Epoxy, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Photopolymer, purl.org/becyt/ford/2 [https], chemistry, visual_art, visual_art.visual_art_medium, EPOXY RESIN, purl.org/becyt/ford/2.5 [https], 0210 nano-technology
Abstract

Photopolymer flexographic printing plate is a new photopolymeric material used for microdevices fabrication. This work demonstrates that a photopolymer flexographic master mold can be used for the fabrication of PDMS (polydimethylsiloxane) microdevices by a multi-step manufacturing process. The methodology entails three main fabrication steps: (1) a photopolymer flexographic printing plate mold (FMold) is generated by UV exposure through a transparent film, (2) an epoxy resin mold (ERmold) is fabricated by transferring the features of the photopolymer mold and (3) a PDMS microdevice is manufactured from the epoxy resin mold. The characterization of the manufactured PDMS microdevices was performed using scanning electron microscopy (SEM) and profilometry. Results showed high accuracy in the replication of the profiles. To show the feasibility of the fabrication process a microdevice for microdroplet generation was designed, manufactured and tested. Hence, the manufacturing process described in this work provides an easy, robust, and low-cost strategy that facilitates the scaling-up of microfluidic devices without requiring any sophisticated equipment. Fil: Olmos Carreno, Carol Maritza. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vaca Mora, Andrea Vanessa. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rosero, Gustavo. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina Fil: Peñaherrera Pazmiño, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina Fil: Pérez Sosa, Camilo José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina Fil: de Sá Carneiro, Igor. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina Fil: Vizuete, Karla. Universidad de Las Fuerzas Armadas Espe; Ecuador Fil: Arroyo, Carlos R.. Universidad de Las Fuerzas Armadas; Ecuador Fil: Debut, Alexis. Universidad de Las Fuerzas Armadas; Ecuador Fil: Perez, Maximiliano Sebastian. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; Argentina Fil: Cumbal, Luis. Universidad de Las Fuerzas Armadas; Ecuador Fil: Lerner, Betiana. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina

Published
2019

4. PDMS-Based Microdevices for the Capture of MicroRNA Biomarkers

Author

Lunelli, Lorenzo, Barbaresco, Federica, Scordo, Giorgio, Potrich, Cristina, Vanzetti, Lia, Marasso, Simone Luigi, Cocuzza, Matteo, Pirri, Candido Fabrizio, Pederzolli, and Cecilia

Subjects
Materials science, CELL LUNG-CANCER, Microfluidics, Nanotechnology, PDMS microdevice, 02 engineering and technology, lcsh:Technology, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, SERUM MICRORNA, General Materials Science, Liquid biopsy, lcsh:QH301-705.5, Instrumentation, 030304 developmental biology, surface functionalization, Fluid Flow and Transfer Processes, 0303 health sciences, PURIFICATION, microRNAs, Polydimethylsiloxane, lcsh:T, CHIP, Process Chemistry and Technology, General Engineering, 021001 nanoscience & nanotechnology, lcsh:QC1-999, Computer Science Applications, Clinical Practice, Circulating biomarkers, lcsh:Biology (General), lcsh:QD1-999, chemistry, lcsh:TA1-2040, POLYDIMETHYLSILOXANE, Non small cell, lcsh:Engineering (General). Civil engineering (General), 0210 nano-technology, lcsh:Physics
Abstract

The isolation and analysis of circulating biomarkers, the main concern of liquid biopsy, could greatly benefit from microfluidics. Microfluidics has indeed the huge potentiality to bring liquid biopsy into the clinical practice. Here, two polydimethylsiloxane (PDMS)-based microdevices are presented as valid tools for capturing microRNAs biomarkers from clinically-relevant samples. After an extensive study of functionalized polydimethylsiloxane (PDMS) properties in adsorbing/eluting microRNAs, the best conditions were transferred to the microdevices, which were thoroughly characterized. The channels morphology and chemical composition were measured, and parameters for the automation of measures were setup. The best working conditions were then used with microdevices, which were proven to capture microRNAs on all channel surfaces. Finally, microfluidic devices were successfully validated via real-time PCR for the detection of a pool of microRNAs related to non-small cell lung cancer, selected as proof-of-principle. The microfluidic approach described here will allow a step forward towards the realization of an efficient microdevice, possibly automated and integrated into a microfluidic lab-on-a-chip with high analytical potentialities.

Published
2020
Full Text
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