1. Isolation of Cyanobacterial Strains from Water and Soil Samples in Hanoi and Investigation of Their Cytotoxic Activity
- Author
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Nguyen Thi Bich Loan, Pham Thi Hang, Ngo Thi Trang, Nguyen Thuy Lien, Tran Thi Hang, Lai Ngoc Khanh, Tran Thi Thuy Anh, Nguyen Thi Thu Lien, and Tong Thi Ngoc Anh
- Subjects
Soil test ,Chemistry ,Cytotoxic T cell ,Isolation (microbiology) ,Microbiology - Abstract
As a part of ongoing efforts to exploit the pharmaceutical potential of domestic cyanobacteria, six strains belonging to the Nostocales order have been isolated from several sampling sites in Ha Noi as prerequisite material. The cytotoxic activity evaluation based on the MTT test resulted in four extracts from two strains (NK7 and NK1111) exhibiting the inhibitory activity against HeLa cells, with IC50 values ranging from 84.6 µg/mL to 257.3 µg/mL. In addition, the bioassay-guided isolation of the HK7 methanol extract led to one cytotoxic activity K3 fraction possessing two natural compounds. Compounds 1 showed the accurate mass of 352.2633 Da with the formula of C21H36O4 similar to three cytotoxic compounds 7Z-plakortide H, 10-gingerdiol, and ebelactone B. Compound 2 had the accurate mass of 278.1545 Da with the formula of C16H22O4 similar to four cytotoxic compounds pestaloficiol G, penicitrinol E-D, and isoversiol C.
- Published
- 2021