33 results on '"Ngandjio A"'
Search Results
2. First report of New Delhi Metallo-Beta-Lactamase-5 (NDM-5) - producing Escherichia coli isolates from water environment in Cameroon
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Megueya, Armelle Leslie, primary, Makuetche, Kévine, additional, Scaccia, Nazareno, additional, Costa, Silvia Figueiredo, additional, Foncesca, Joyce Vanessa da Silva, additional, Komurian–Pradel, Florence, additional, Tchatchouang, Serges, additional, Simo, Pierrette, additional, Tchatchueng, Jules, additional, Nono, Arsène Djoko, additional, Ngang, Essia, additional, Ngandjio, Antoinette, additional, and Nzouankeu, Ariane, additional
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- 2023
- Full Text
- View/download PDF
3. First report of New Delhi metallo-beta-lactamase-5 (NDM-5)-producing Escherichia coli isolates from water environment in Cameroon
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Megueya, Armelle Leslie, Makuetche, Kévine, Scaccia, Nazareno, Costa, Silvia Figueiredo, da Silva Foncesca, Joyce Vanessa, Komurian-Pradel, Florence, Tchatchouang, Serges, Simo, Pierrette, Tchatchueng, Jules, Nono, Arsène Djoko, Ngang, Essia, Ngandjio, Antoinette, and Nzouankeu, Ariane
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- 2024
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4. Author Correction: Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance (Nature Communications, (2022), 13, 1, (7251), 10.1038/s41467-022-34312-7)
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Munk, Patrick, Brinch, Christian, Møller, Frederik Duus, Petersen, Thomas N., Hendriksen, Rene S., Seyfarth, Anne Mette, Kjeldgaard, Jette S., Svendsen, Christina Aaby, van Bunnik, Bram, Berglund, Fanny, Bego, Artan, Power, Pablo, Rees, Catherine, Lambrinidis, Dionisia, Neilson, Elizabeth Heather Jakobsen, Gibb, Karen, Coventry, Kris, Collignon, Peter, Cassar, Susan, Allerberger, Franz, Begum, Anowara, Hossain, Zenat Zebin, Worrell, Carlon, Vandenberg, Olivier, Pieters, Ilse, Victorien, Dougnon Tamègnon, Gutierrez, Angela Daniela Salazar, Soria, Freddy, Grujić, Vesna Rudić, Mazalica, Nataša, Rahube, Teddie O., Tagliati, Carlos Alberto, Rodrigues, Dalia, Oliveira, Guilherme, de Souza, Larissa Camila Ribeiro, Ivanov, Ivan, Juste, Bonkoungou Isidore, Oumar, Traoré, Sopheak, Thet, Vuthy, Yith, Ngandjio, Antoinette, Nzouankeu, Ariane, Olivier, Ziem A.Abah Jacques, Yost, Christopher K., Kumar, Pratik, Brar, Satinder Kaur, Tabo, Djim Adjim, Adell, Aiko D., Paredes-Osses, Esteban, Koopmans, Marion, and Virology
- Abstract
In this article, the author name Antoinette Ngandjio was incorrectly written as Antoinette Ngandijo. In this article, the affiliation details for Author Sara Cuadros-Orellana were incorrectly given as ‘Centro de Biotecnologνa de los Recursos Naturales, Facultad de Ciencias Agrarias y Forestales, Talca, Chile’ but should have been ‘Universidad Catolica del Maule, Centro de Biotecnología de los Recursos Naturales, Facultad de Ciencias Agrarias y Forestales, Talca, Chile’. The original article has been corrected.
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- 2023
5. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa
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Murielle Baltazar, Antoinette Ngandjio, Kathryn Elizabeth Holt, Elodie Lepillet, Maria Pardos de la Gandara, Jean-Marc Collard, Raymond Bercion, Ariane Nzouankeu, Simon Le Hello, Gordon Dougan, Marie-Christine Fonkoua, and François-Xavier Weill
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Salmonella enterica serotype Typhi ,bacteria ,multidrug resistance ,MDR ,pulsed-field gel electrophoresis ,PFGE ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
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- 2015
- Full Text
- View/download PDF
6. Histoplasmosis in HIV-Infected Persons, Yaoundé, Cameroon
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Christine E. Mandengue, Antoinette Ngandjio, and Paul J.A. Atangana
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Cameroon ,HIV/AIDS and other retroviruses ,tuberculosis ,histoplasmosis ,fungi ,viruses ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2015
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7. Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258
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Breurec, S., Guessennd, N., Timinouni, M., Le, T.T.H., Cao, V., Ngandjio, A., Randrianirina, F., Thiberge, J.M., Kinana, A., Dufougeray, A., Perrier-Gros-Claude, J.D., Boisier, P., Garin, B., and Brisse, S.
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- 2013
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8. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010.
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Rolf S Kaas, Antoinette Ngandjio, Ariane Nzouankeu, Achiraya Siriphap, Marie-Christine Fonkoua, Frank M Aarestrup, and Rene S Hendriksen
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Medicine ,Science - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
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- 2016
- Full Text
- View/download PDF
9. Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017
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Marie-Christine Fonkoua, M. Massongo, W. Mbanzouen, D. Barger, L. Ngando, Ariane Nzouankeu, J. Tchatchueng, E.W. Pefura Yone, M. C. Tejiokem, A. Ngandjio, Université de Yaoundé I, Jamot hospital, Service de bactériologie [Yaoundé, Cameroun], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire d'Epidémiologie et de Santé Publique, Bordeaux population health (BPH), and Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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0301 basic medicine ,Adult ,Male ,Veterinary medicine ,Salmonella ,Article Subject ,Adolescent ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Drug resistance ,Microbial Sensitivity Tests ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Drug Resistance, Bacterial ,medicine ,Humans ,030212 general & internal medicine ,Cameroon ,Medical prescription ,Child ,Retrospective Studies ,General Immunology and Microbiology ,biology ,Bacteria ,Pseudomonas aeruginosa ,business.industry ,Infant ,General Medicine ,Bacterial Infections ,Acinetobacter ,Middle Aged ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Anti-Bacterial Agents ,Multiple drug resistance ,Child, Preschool ,Medicine ,Female ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,business ,Research Article - Abstract
Introduction. Antimicrobial resistance represents a growing public health threat. One of the World Health Organization’s strategic objectives is “strengthening knowledge through surveillance and research.” Sub-Saharan African countries are still far from achieving this objective. We aimed to estimate and compare the prevalence of antibacterial resistance in 2010 and 2017 in Cameroon. Methods. We conducted a retrospective study on all clinical specimens cultured in Centre Pasteur du Cameroun (CPC) in 2010 and 2017. Data were extracted from the CPC’s laboratory data information system software and then managed and analyzed using R. Bacterial resistance rates were calculated in each year and compared using chi-square or Fisher’s tests, and relative changes were calculated. Outcomes included acquired resistance (AR), WHO priority resistant pathogens, some specific resistances of clinical interest, and resistance patterns (multi, extensively, and pan drug resistances) for five selected pathogens. Results. A total of 10,218 isolates were analyzed. The overall AR rate was 96.0% (95% CI: 95.4–96.6). Most of WHO priority bacterial resistance rates increased from 2010 to 2017. The most marked increases expressed as relative changes concerned imipenem-resistant Acinetobacter (6.2% vs. 21.6%, +248.4%, p = 0.02 ), imipenem-resistant Pseudomonas aeruginosa (13.5% vs. 23.5%, +74.1%, p < 0.01 ), 3rd generation-resistant Enterobacteriaceae (23.8% vs. 40.4%, +65.8%, p < 10 − 15 ), methicillin-resistant Staphylococcus aureus (27.3% vs. 46.0%, +68.6%, p < 0.002 ), fluoroquinolone-resistant Salmonella (3.9% vs. 9.5%, +142.9%, p = 0.03 ), and fluoroquinolone-resistant Enterobacteriaceae (32.6% vs. 54.0%, +65.8%, p < 10 − 15 ). For selected pathogens, global multidrug resistance was high in 2010 and 2017 (74.9% vs. 78.0% +4.1%, p = 0.01 ), intensively drug resistance rate was 5.8% (7.0% vs. 4.7%; p = 0.07 ), and no pan drug resistance has been identified. Conclusion. Bacterial resistance to antibiotics of clinical relevance in Cameroon was high and appeared to increase between 2010 and 2017. There is a need for regular surveillance of antibacterial resistance to inform public health strategies and empirically inform prescription practices.
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- 2021
10. Genomic history of the seventh pandemic of cholera in Africa
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François-Xavier Weill, Karen H. Keddy, Mireille Dosso, Sandra Moore, Anne Laure Page, Renaud Piarroux, Jean Michel Fournier, Antoinette Ngandjio, Monakhova Ev, Julian Parkhill, Sébastien Breurec, Monzer Hamze, Nizar Fawal, Henrik Salje, Fati Sidikou, Elisabeth Njamkepo, Asish K. Mukhopadhyay, Maria Damian, Gordon Dougan, Kathryn E. Holt, Marie Laure Quilici, Daryl Domman, Raymond Bercion, Jean Rauzier, Martin A. Mengel, Cheryl L. Tarr, Patrick A. D. Grimont, Benoit Garin, Laurence Bonte, Goutam Chowdhury, Roland Grunow, Jean Louis Koeck, Berthe Marie Njanpop-Lafourcade, Guillaume Sapriel, Myriam Henkens, Carlo Pazzani, Christiane Bouchier, Ankur Mutreja, Nicholas R. Thomson, Francisco J. Luquero, Thandavarayan Ramamurthy, Bactéries pathogènes entériques (BPE), Institut Pasteur [Paris] (IP), The Wellcome Trust Sanger Institute [Cambridge], Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, National Institute for Communicable Diseases [Johannesburg] (NICD), University of the Witwatersrand [Johannesburg] (WITS), Modélisation mathématique des maladies infectieuses - Mathematical modelling of Infectious Diseases, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), Hôpital de la Timone [CHU - APHM] (TIMONE), Université de la Méditerranée - Aix-Marseille 2, Institut Pasteur de Bangui, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Dakar, Epicentre [Paris] [Médecins Sans Frontières], Centre Pasteur du Cameroun, Institut Pasteur de Côte d'Ivoire, Rostov-on-Don Research Institute for Plague Control, Génomique (Plate-Forme) - Genomics Platform, Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), University of Cambridge [UK] (CAM), Translational Health Science and Technology Institute [Faridabad] (THSTI), Robert Koch Institute [Berlin] (RKI), Centre de Recherche Médicale et Sanitaire (Niamey, Niger) (CERMES), Cantacuzino Institute [Romania], Agence de Médecine Préventive, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Université Libanaise, Médecins Sans Frontières Belgique, National Institute of Cholera and Enteric Diseases, Hôpital Bouffard - Centre Hospitalier des Armées Bouffard [Djibouti], Service de Santé des Armées, Centre National de Référence des Vibrions et du Choléra - National Reference Center Vibrios and Cholera (CNR), Biodiversité des Bactéries Pathogènes Émergentes, University of Melbourne, London School of Hygiene and Tropical Medicine (LSHTM), This study was supported by the Institut Pasteur and the Institut Pasteur International Network, the Institut de Veille Sanitaire, the French government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant no. ANR-10-LABX-62-IBEID), the Fondation Le Roch-Les Mousquetaires, the Wellcome Trust through grant 098051 to the Sanger Institute, and the Indian Council of Medical Research, New Delhi, India., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), University of Bari Aldo Moro (UNIBA), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Weill, François-Xavier [0000-0001-9941-5799], Domman, Daryl [0000-0001-5389-9185], Njamkepo, Elisabeth [0000-0001-6791-6003], Tarr, Cheryl [0000-0001-6290-365X], Fawal, Nizar [0000-0002-9914-0315], Keddy, Karen H [0000-0001-8640-2235], Salje, Henrik [0000-0003-3626-4254], Bercion, Raymond [0000-0002-0466-0407], Luquero, Francisco J [0000-0003-0885-8418], Ngandjio, Antoinette [0000-0001-9777-3656], Dosso, Mireille [0000-0003-4744-8235], Monakhova, Elena [0000-0002-9216-7777], Bouchier, Christiane [0000-0003-3549-1543], Pazzani, Carlo [0000-0003-0414-2965], Grunow, Roland [0000-0001-8966-8469], Sidikou, Fati [0000-0002-0269-5454], Njanpop-Lafourcade, Berthe-Marie [0000-0001-6211-6402], Page, Anne-Laure [0000-0001-7952-3178], Koeck, Jean-Louis [0000-0002-6493-3905], Dougan, Gordon [0000-0003-0022-965X], Grimont, Patrick AD [0000-0002-6264-136X], Parkhill, Julian [0000-0002-7069-5958], Holt, Kathryn E [0000-0003-3949-2471], Piarroux, Renaud [0000-0002-4151-4134], Thomson, Nicholas R [0000-0002-4432-8505], and Apollo - University of Cambridge Repository
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0301 basic medicine ,MESH: Pandemics ,Asia ,Lineage (genetic) ,Genomic data ,Zoology ,MESH: Vibrio cholerae O1 ,Biology ,MESH: Genome, Bacterial ,medicine.disease_cause ,Africa, Southern ,West africa ,03 medical and health sciences ,Cholera ,MESH: Cholera ,Phylogenetics ,MESH: Africa, Western ,parasitic diseases ,Pandemic ,medicine ,Humans ,MESH: Phylogeny ,Pandemics ,Phylogeny ,MESH: Africa, Eastern ,MESH: Humans ,Multidisciplinary ,MESH: Asia ,Transmission (medicine) ,MESH: Genomics ,Vibrio cholerae O1 ,Genomics ,Africa, Eastern ,medicine.disease ,3. Good health ,Africa, Western ,030104 developmental biology ,Vibrio cholerae ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH: Africa, Southern ,Genome, Bacterial - Abstract
Wave upon wave of diseaseThe cholera pathogen,Vibrio cholerae, is considered to be ubiquitous in water systems, making the design of eradication measures apparently fruitless. Nevertheless, local and globalVibriopopulations remain distinct. Now, Weillet al.and Dommanet al.show that a surprising diversity between continents has been established. Latin America and Africa bear different variants of cholera toxin with different transmission dynamics and ecological niches. The data are not consistent with the establishment of long-term reservoirs of pandemic cholera or with a relationship to climate events.Science, this issue p.785, p.789
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- 2017
11. Histoplasmosis in HIV-infected persons, Yaounde, Cameroon
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Mandengue, Christine E., Ngandjio, Antoinette, and Atangana, Paul J.A.
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HIV patients -- Health aspects ,Histoplasmosis -- Health aspects ,Health - Abstract
To the Editor: In HIV-infected persons in Cameroon (Central Africa), histoplasmosis is still misdiagnosed as tuberculosis because of clinical similarities (1,2). These patients are automatically given presumptive antituberculous therapy, although [...]
- Published
- 2015
12. Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017
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Massongo, M., primary, Ngando, L., additional, Pefura Yone, E. W., additional, NZouankeu, Ariane, additional, Mbanzouen, W., additional, Fonkoua, M. C., additional, Ngandjio, A., additional, Tchatchueng, J., additional, Barger, D., additional, and Tejiokem, M. C., additional
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- 2021
- Full Text
- View/download PDF
13. Trends in Gonococcal Antimicrobial Resistance in Cameroon: Before and After the Launching of the Gonococcal Antimicrobial Surveillance Program
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Esther Sokeng, Carole Tayimetha, Ariane Nzouankeu, Marcelle Abanda, Marie-Christine Fonkoua, Gaëlle Tchouwa, and Antoinette Ngandjio
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Antibiotic resistance ,business.industry ,Medicine ,Antimicrobial ,business ,Microbiology - Published
- 2016
14. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students
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Ngandjio, Antoinette, Clerc, Maithe, Fonkoua, Marie Christine, Thonnon, Jocelyn, Lunel, Françoise, Bébéar, Christiane, Bianchi, Anne, and de Barbeyrac, Bertille
- Published
- 2004
15. Erratum: Global phylogeography and evolutionary history of Shigella dysenteriae type 1
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Elisabeth Njamkepo, Nizar Fawal, Alicia Tran-Dien, Jane Hawkey, Nancy Strockbine, Claire Jenkins, Kaisar A. Talukder, Raymond Bercion, Konstantin Kuleshov, Renáta Kolínská, Julie E. Russell, Lidia Kaftyreva, Marie Accou-Demartin, Andreas Karas, Olivier Vandenberg, Alison E. Mather, Carl J. Mason, Andrew J. Page, Thandavarayan Ramamurthy, Chantal Bizet, Andrzej Gamian, Isabelle Carle, Amy Gassama Sow, Christiane Bouchier, Astrid Louise Wester, Monique Lejay-Collin, Marie-Christine Fonkoua, Simon Le Hello, Martin J. Blaser, Cecilia Jernberg, Corinne Ruckly, Audrey Mérens, Anne-Laure Page, Martin Aslett, Peter Roggentin, Angelika Fruth, Erick Denamur, Malabi Venkatesan, Hervé Bercovier, Ladaporn Bodhidatta, Chien-Shun Chiou, Dominique Clermont, Bianca Colonna, Svetlana Egorova, Gururaja P. Pazhani, Analia V. Ezernitchi, Ghislaine Guigon, Simon R. Harris, Hidemasa Izumiya, Agnieszka Korzeniowska-Kowal, Anna Lutyńska, Malika Gouali, Francine Grimont, Céline Langendorf, Monika Marejková, Lorea A.M. Peterson, Guillermo Perez-Perez, Antoinette Ngandjio, Alexander Podkolzin, Erika Souche, Mariia Makarova, German A. Shipulin, Changyun Ye, Helena Žemličková, Mária Herpay, Patrick A. D. Grimont, Julian Parkhill, Philippe Sansonetti, Kathryn E. Holt, Sylvain Brisse, Nicholas R. Thomson, and François-Xavier Weill
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Microbiology (medical) ,Immunology ,Genetics ,Cell Biology ,Applied Microbiology and Biotechnology ,Microbiology - Published
- 2016
16. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
- Author
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Frank Møller Aarestrup, Antoinette Ngandjio, Rene S. Hendriksen, Achiraya Siriphap, Ariane Nzouankeu, Marie-Christine Fonkoua, and Rolf Sommer Kaas
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Bacterial Diseases ,0301 basic medicine ,Disease reservoir ,Marine and Aquatic Sciences ,lcsh:Medicine ,Plant Science ,Pathology and Laboratory Medicine ,Plant Genetics ,medicine.disease_cause ,El Tor ,Genome ,Disease Outbreaks ,Geographical Locations ,Cholera ,Medicine and Health Sciences ,Plant Genomics ,Cameroon ,lcsh:Science ,Phylogeny ,Genetics ,Multidisciplinary ,biology ,Vibrio cholerae O1 ,Genomics ,Anti-Bacterial Agents ,Bacterial Pathogens ,Infectious Diseases ,Medical Microbiology ,Vibrio cholerae ,Pathogens ,Research Article ,Neglected Tropical Diseases ,Freshwater Environments ,Biotechnology ,Genotype ,Chad ,Microbial Sensitivity Tests ,Serogroup ,History, 21st Century ,Polymorphism, Single Nucleotide ,Microbiology ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,Phylogenetics ,Microbial Control ,Vibrio Cholerae ,parasitic diseases ,medicine ,Humans ,Microbial Pathogens ,Disease Reservoirs ,Vibrio ,Pharmacology ,Bacteria ,Ecology and Environmental Sciences ,lcsh:R ,Organisms ,Biology and Life Sciences ,Aquatic Environments ,Outbreak ,Sequence Analysis, DNA ,Bodies of Water ,Tropical Diseases ,medicine.disease ,biology.organism_classification ,Lakes ,030104 developmental biology ,People and Places ,Africa ,Earth Sciences ,Multilocus sequence typing ,Plant Biotechnology ,lcsh:Q ,Antimicrobial Resistance ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
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- 2016
17. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa
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Pardos de la Gandara, Maria, Baltazar, Murielle, Ngandjio, Antoinette, Holt, Kathryn Elizabeth, Lepillet, Elodie, Pardos De La Gándara, María, Collard, Jean-Marc, Bercion, Raymond, Nzouankeu, Ariane, Le Hello, Simon, Dougan, Gordon, Fonkoua, Marie-Christine, Weill, François-Xavier, Institut Pasteur [Paris], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), University of Melbourne, The Wellcome Trust Sanger Institute [Cambridge], Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS), Institut Scientifique de Santé Publique [Belgique] - Scientific Institute of Public Health [Belgium] (WIV-ISP), Institut Pasteur de Bangui, This study was supported by the Institut Pasteur, the Réseau International des Instituts Pasteur, the Institut de Veille Sanitaire, and the French Government Investissement d’Avenir Program (Integrative Biology of Emerging Infectious Diseases, Laboratory of Excellence, grant ANR-10-LABX-62-IBEID)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris] (IP)
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Serotype ,MESH: Geography ,Epidemiology ,lcsh:Medicine ,Salmonella enterica serotype Typhi ,Drug resistance ,Salmonella typhi ,Plasmid ,Drug Resistance, Multiple, Bacterial ,MDR ,MESH: Genetic Variation ,bacteria ,0303 health sciences ,MESH: Guinea ,MESH: Microbial Sensitivity Tests ,Geography ,biology ,MESH: Polymorphism, Single Nucleotide ,MESH: Salmonella typhi ,Dispatch ,PFGE ,Anti-Bacterial Agents ,Infectious Diseases ,Salmonella enterica ,pulsed-field gel electrophoresis ,Microbiology (medical) ,Microbial Sensitivity Tests ,Polymorphism, Single Nucleotide ,Typhoid fever ,Microbiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,multidrug resistance ,MESH: Anti-Bacterial Agents ,medicine ,Humans ,lcsh:RC109-216 ,MESH: Typhoid Fever ,Typhoid Fever ,030304 developmental biology ,Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa ,MESH: Humans ,030306 microbiology ,Haplotype ,lcsh:R ,Genetic Variation ,MESH: Drug Resistance, Multiple, Bacterial ,MESH: Haplotypes ,biology.organism_classification ,medicine.disease ,Virology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Multiple drug resistance ,Haplotypes ,Guinea ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
- Published
- 2015
18. Erratum: Global phylogeography and evolutionary history of Shigella dysenteriae type 1
- Author
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Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A, Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E, Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison AE, Mason, Carl J, Page, Andrew J, Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carles, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Hello, Simon Le, Blaser, Martin J, Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja GP, Ezernitchi, Analia V, Guigon, Ghislaine, Harris, Simon R, Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A M LA, Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A, Ye, Changyun, Zemlickova, Helena, Herpay, Mária, Grimont, Patrick A D PA, Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn KE, Brisse, Sylvain, Thomson, Nicholas R, Weill, François-Xavier, Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A, Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E, Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison AE, Mason, Carl J, Page, Andrew J, Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carles, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Hello, Simon Le, Blaser, Martin J, Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja GP, Ezernitchi, Analia V, Guigon, Ghislaine, Harris, Simon R, Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A M LA, Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A, Ye, Changyun, Zemlickova, Helena, Herpay, Mária, Grimont, Patrick A D PA, Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn KE, Brisse, Sylvain, Thomson, Nicholas R, and Weill, François-Xavier
- Abstract
info:eu-repo/semantics/published
- Published
- 2016
19. Global phylogeography and evolutionary history of Shigella dysenteriae type 1
- Author
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Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A, Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E, Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison AE, Mason, Carl J, Page, Andrew J, Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carles, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Hello, Simon Le, Blaser, Martin J, Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja GP, Ezernitchi, Analia V, Guigon, Ghislaine, Harris, Simon R, Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A M LA, Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A, Ye, Changyun, Zemlickova, Helena, Herpay, Mária, Grimont, Patrick A D PA, Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn KE, Brisse, Sylvain, Thomson, Nicholas R, Weill, François-Xavier, Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A, Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E, Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison AE, Mason, Carl J, Page, Andrew J, Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carles, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Hello, Simon Le, Blaser, Martin J, Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja GP, Ezernitchi, Analia V, Guigon, Ghislaine, Harris, Simon R, Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A M LA, Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A, Ye, Changyun, Zemlickova, Helena, Herpay, Mária, Grimont, Patrick A D PA, Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn KE, Brisse, Sylvain, Thomson, Nicholas R, and Weill, François-Xavier
- Abstract
Together with plague, smallpox and typhus, epidemics of dysentery have been a major scourge of human populations for centuries(1). A previous genomic study concluded that Shigella dysenteriae type 1 (Sd1), the epidemic dysentery bacillus, emerged and spread worldwide after the First World War, with no clear pattern of transmission(2). This is not consistent with the massive cyclic dysentery epidemics reported in Europe during the eighteenth and nineteenth centuries(1,3,4) and the first isolation of Sd1 in Japan in 1897(5). Here, we report a whole-genome analysis of 331 Sd1 isolates from around the world, collected between 1915 and 2011, providing us with unprecedented insight into the historical spread of this pathogen. We show here that Sd1 has existed since at least the eighteenth century and that it swept the globe at the end of the nineteenth century, diversifying into distinct lineages associated with the First World War, Second World War and various conflicts or natural disasters across Africa, Asia and Central America. We also provide a unique historical perspective on the evolution of antibiotic resistance over a 100-year period, beginning decades before the antibiotic era, and identify a prevalent multiple antibiotic-resistant lineage in South Asia that was transmitted in several waves to Africa, where it caused severe outbreaks of disease., SCOPUS: ar.j, info:eu-repo/semantics/published
- Published
- 2016
20. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
- Author
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Kaas, Rolf Sommer, Ngandjio, Antoinette, Nzouankeu, Ariane, Siriphap, Achiraya, Fonkoua, Marie-Christine, Aarestrup, Frank Møller, Hendriksen, Rene S., Kaas, Rolf Sommer, Ngandjio, Antoinette, Nzouankeu, Ariane, Siriphap, Achiraya, Fonkoua, Marie-Christine, Aarestrup, Frank Møller, and Hendriksen, Rene S.
- Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
- Published
- 2016
21. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students
- Author
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Anne Bianchi, Marie Christine Fonkoua, Antoinette Ngandjio, Bertille de Barbeyrac, Christiane Bébéar, Jocelyn Thonnon, Françoise Lunel, and M. Clerc
- Subjects
Microbiology (medical) ,Genotype ,HpaII ,Restriction Mapping ,EcoRI ,Porins ,Chlamydia trachomatis ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Microbiology ,Male Urogenital Diseases ,medicine ,Humans ,Cameroon ,Typing ,Genetics ,General Medicine ,Chlamydia Infections ,Female Urogenital Diseases ,Restriction enzyme ,biology.protein ,Restriction fragment length polymorphism ,Nested polymerase chain reaction ,Polymorphism, Restriction Fragment Length - Abstract
Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR- and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification of 15 different patterns. In all, 16/18 reference strains were clearly identified. These reference patterns were successfully used to genotype 34 of 35 (28 strains and 7 clinical specimens) samples from infected students, collected during a screening programme in Yaounde (Cameroon). Genotypes D, Da, E, F, G and J were found. The most prevalent omp1 genotype was E (n = 14; 40 %), followed by F (n = 7; 20 %). As RFLP patterns of reference strains are essential for typing clinical isolates, they will greatly facilitate C. trachomatis characterization in many resource-limited laboratories.
- Published
- 2004
22. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
- Author
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Kaas, Rolf S., primary, Ngandjio, Antoinette, additional, Nzouankeu, Ariane, additional, Siriphap, Achiraya, additional, Fonkoua, Marie-Christine, additional, Aarestrup, Frank M., additional, and Hendriksen, Rene S., additional
- Published
- 2016
- Full Text
- View/download PDF
23. Multidrug-Resistant Salmonella enterica, Serotype Typhi, Gulf of Guinea Region, Africa
- Author
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Baltazar, M, Ngandjio, A, Holt, KE, Lepillet, E, Pardos de la Gandara, M, Collard, J-M, Bercion, R, Nzouankeu, A, Le Hello, S, Dougan, G, Fonkoua, M-C, Weill, F-X, Baltazar, M, Ngandjio, A, Holt, KE, Lepillet, E, Pardos de la Gandara, M, Collard, J-M, Bercion, R, Nzouankeu, A, Le Hello, S, Dougan, G, Fonkoua, M-C, and Weill, F-X
- Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
- Published
- 2015
24. Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258
- Author
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Jean-David Perrier-Gros-Claude, V Cao, T A H Le, Benoit Garin, N Guessennd, Sylvain Brisse, M Timinouni, A Ngandjio, Jean-Michel Thiberge, Frédérique Randrianirina, Alfred Dieudonné Kinana, P. Boisier, A. Dufougeray, Sébastien Breurec, Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Côte d'Ivoire, Institut Pasteur du Maroc, Institut National d'Hygiène et d'Épidémiologie de Hanoi (NIHE), Institut Pasteur d'Ho Chi Minh Ville, Centre Pasteur du Cameroun, Institut Pasteur de Madagascar, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Institut Pasteur [Paris], We thank Fatou Bintou Dieye (Institut Pasteur, Dakar, Senegal), Laure Diancourt and Patrice Courvalin (Institut Pasteur, Paris, France), Guillaume Arlet (Hoˆ pital Tenon, Paris, France) and Marie Christine Ploy (CHU Dupuytren, Limoges, France) for their contributions, as well as all the clinicians involved in this study., and Institut Pasteur [Paris] (IP)
- Subjects
Microbiology (medical) ,CTX-M-15 ,Veterinary medicine ,Genotype ,Klebsiella pneumoniae ,multilocus sequence typing ,beta-Lactam Resistance ,Microbiology ,03 medical and health sciences ,Intergenic region ,Antibiotic resistance ,Genetic variation ,parasitic diseases ,Humans ,antimicrobial resistance ,Developing Countries ,030304 developmental biology ,CG15 ,0303 health sciences ,Genetic diversity ,Molecular Epidemiology ,Molecular epidemiology ,biology ,030306 microbiology ,1. No poverty ,Genetic Variation ,General Medicine ,extended-spectrum β-lactamase ,biology.organism_classification ,extended-spectrum b-lactamase ,3. Good health ,Anti-Bacterial Agents ,Cephalosporins ,Klebsiella Infections ,Infectious Diseases ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Vietnam ,Genes, Bacterial ,Africa ,CG258 ,Multilocus sequence typing ,epidemiology ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; The molecular epidemiology of third-generation cephalosporin-resistant (3GC-R) Klebsiella pneumoniae in developing countries is poorly documented. From February 2007 to March 2008, we collected 135 3GC-R K. pneumoniae isolates from seven major towns in Maghreb (Morocco), West Africa (Senegal, Côte d'Ivoire), Central Africa (Cameroon), East Africa (Madagascar) and Southeast Asia (Vietnam). Their genetic diversity, assessed by multilocus sequence typing, was high (60 sequence types), reflecting multiclonality. However, two major clonal groups, CG15 (n = 23, 17% of isolates) and CG258 (n = 18, 13%), were detected in almost all participating centres. The two major clonal groups have previously been described in other parts of the world, indicating their global spread. The high diversity of enterobacterial repetitive intergenic consensus sequence-PCR banding patterns at the local level indicates that most isolates were epidemiologically unrelated. The isolates were characterized by the presence of multiple resistance determinants, most notably the concomitant presence of the aac(6')-Ib-cr, qnr and bla(CTX-M-15) genes in 61 isolates (45%) belonging to 31 sequence types. These isolates were detected across a large geographical area including Cameroon (n = 1), Vietnam (n = 4), Madagascar (n = 10), Côte d'Ivoire (n = 12), Morocco (n = 13) and Senegal (n = 21). These results have major implications for patient management and highlight a potential reservoir for resistance determinants.
- Published
- 2012
25. Emergence of multi-drug resistant Salmonella enterica serotype Stanleyville infections among children in Yaounde, Cameroon
- Author
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Paul Koki Ndombo, Antoinette Ngandjio, Marie-Christine Fonkoua, Hortense Gonsu Kamga, and Patrice Tchendjou
- Subjects
Serotype ,Male ,biology ,Adolescent ,business.industry ,Infant ,Salmonella enterica ,biology.organism_classification ,Virology ,Drug Resistance, Multiple ,Microbiology ,Infectious Diseases ,Anti-Infective Agents ,Pediatrics, Perinatology and Child Health ,Salmonella Infections ,Multi drug resistant ,Medicine ,Humans ,Female ,Cameroon ,Serotyping ,business - Published
- 2011
26. Multidrug-ResistantSalmonella entericaSerotype Typhi, Gulf of Guinea Region, Africa
- Author
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Baltazar, Murielle, primary, Ngandjio, Antoinette, additional, Holt, Kathryn Elizabeth, additional, Lepillet, Elodie, additional, Pardos de la Gandara, Maria, additional, Collard, Jean-Marc, additional, Bercion, Raymond, additional, Nzouankeu, Ariane, additional, Le Hello, Simon, additional, Dougan, Gordon, additional, Fonkoua, Marie-Christine, additional, and Weill, François-Xavier, additional
- Published
- 2015
- Full Text
- View/download PDF
27. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatis Infection and Genotyping of Isolated C. trachomatis Strains
- Author
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Françoise Lunel, Jocelyn Thonnon, Antoinette Ngandjio, Friede Njock, Marie Christine Fonkoua, Régis Pouillot, Christiane Bébéar, Bertille de Barbeyrac, M. Clerc, and Anne Bianchi
- Subjects
Microbiology (medical) ,Sexually transmitted disease ,Adult ,Male ,Genotype ,Chlamydiology and Rickettsiology ,Porins ,Chlamydia trachomatis ,medicine.disease_cause ,Risk Factors ,Prevalence ,Medicine ,Humans ,Chlamydiaceae ,Cameroon ,Genotyping ,Chlamydia ,Molecular epidemiology ,biology ,business.industry ,Chlamydia Infections ,biology.organism_classification ,medicine.disease ,Virology ,Chlamydiales ,Female ,business - Abstract
The prevalence of Chlamydia trachomatis infection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women ( P = 0.026) and a previous sexually transmitted infection in men ( P = 0.023). The most frequent genotypes determined by sequencing the omp1 genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
- Published
- 2003
28. Multiple contaminations of chickens with Campylobacter, Escherichia coli and Salmonella in Yaounde (Cameroon)
- Author
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Nzouankeu, Ariane, primary, Ngandjio, Antoinette, additional, Ejenguele, Guy, additional, Njine, Thomas, additional, and Ndayo Wouafo, Marguerite, additional
- Published
- 2010
- Full Text
- View/download PDF
29. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa.
- Author
-
Baltazar, Murielle, Ngandjio, Antoinette, Holt, Kathryn Elizabeth, Lepillet, Elodie, Pardos de la Gandara, Maria, Collard, Jean-Marc, Bercion, Raymond, Nzouankeu, Ariane, Le Hello, Simon, Dougan, Gordon, Fonkoua, Marie-Christine, and Weill, François-Xavier
- Subjects
- *
MULTIDRUG resistance in bacteria , *SALMONELLA enterica serovar Typhi , *HAPLOTYPES , *PLASMIDS - Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
30. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatis Infection and Genotyping of Isolated C. trachomatis Strains
- Author
-
Ngandjio, Antoinette, primary, Clerc, Maithe, additional, Fonkoua, Marie Christine, additional, Thonnon, Jocelyn, additional, Njock, Friede, additional, Pouillot, Regis, additional, Lunel, Françoise, additional, Bebear, Christiane, additional, de Barbeyrac, Bertille, additional, and Bianchi, Anne, additional
- Published
- 2003
- Full Text
- View/download PDF
31. First report of New Delhi metallo-beta-lactamase-5 (NDM-5)-producing Escherichia coliisolates from water environment in Cameroon
- Author
-
Megueya, Armelle Leslie, Makuetche, Kévine, Scaccia, Nazareno, Costa, Silvia Figueiredo, da Silva Foncesca, Joyce Vanessa, Komurian-Pradel, Florence, Tchatchouang, Serges, Simo, Pierrette, Tchatchueng, Jules, Nono, Arsène Djoko, Ngang, Essia, Ngandjio, Antoinette, and Nzouankeu, Ariane
- Published
- 2023
- Full Text
- View/download PDF
32. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatisInfection and Genotyping of Isolated C. trachomatisStrains
- Author
-
Ngandjio, Antoinette, Clerc, Maithe, Fonkoua, Marie Christine, Thonnon, Jocelyn, Njock, Friede, Pouillot, Regis, Lunel, Franc¸oise, Bebear, Christiane, de Barbeyrac, Bertille, and Bianchi, Anne
- Abstract
ABSTRACTThe prevalence of Chlamydia trachomatisinfection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women (P= 0.026) and a previous sexually transmitted infection in men (P= 0.023). The most frequent genotypes determined by sequencing the omp1genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
- Published
- 2003
- Full Text
- View/download PDF
33. Intégrons de classe 3 : aspects mécanistiques et épidémiologiques
- Author
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Simo Tchuinte, Pierrette Landrie, STAR, ABES, Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Université de Limoges, Marie-Cécile Ploy, Antoinette Brigitte Chikaha Tchouya-Ngandjio, and Olivier Barraud
- Subjects
Resistance aux antibiotiques ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,Class 3 integrons ,Antibiotic resistance ,Effluents ,Intégrons de classe 3 ,Delftia ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Integrons are bacterial genetic elements able to capture and express genes embedded within gene cassettes. They are widely described among Gram-negative bacteria and generally confer a multidrug resistance phenotype. Resistance integrons (RI) play an important role in the acquisition of antibiotic resistance. There are 3 main classes of RI. Class 3 RI has been poorly studied class with less than ten fully IR3 characterized. Objectives of this thesis were (i) to conduct an epidemiological study of class 3 RI in France and Cameroon and (ii) to better understand the modes of expression of the integrase and cassettes of IR3. We described 3 new class 3 RI isolated from environmental bacteria belonging to genus Aeromonas, Acinetobacter and Citrobacter. Gene cassettes encoded resistance to betalactams, aminoglycosides and quaternary ammonium compounds. We also described IR3 from three Delftia strains (2 D.acidovorans and 1 D.tsuruhatensis) in Africa containing cassettes that do not encode antibiotic resistance. The fundamental part of the work showed that the PintI3(1) promoter is involved in the expression of the intI3 gene. Furthermore, we demonstrated that variants of the Pc promoter and variants of the PintI3(1) promoter are functional with different strengths. These results showed that the environment may constitute a reservoir of class 3 integrons and that these genetic elements could play an important role in the spread of the resistance in this ecosystem., Les intégrons sont des supports génétiques bactériens de capture, d’expression et de dissémination de gènes de résistance aux antibiotiques sous forme de cassettes. Ils sont majoritairement décrits chez les bactéries à Gram négatif chez qui ils confèrent généralement un phénotype de multirésistance. Les intégrons de résistance (IR) jouent un rôle majeur dans l’acquisition de la résistance dans le monde bactérien. Il existe 3 principales classes d’IR ; les IR de classe 1, les IR de classe 2 et les IR de classe 3 (IR3). Contrairement aux 2 premières classes, les IR3 représentent la classe d’intégrons de résistance la moins étudiée. Très peu de travaux s’intéressent à leur étude et on dénombre actuellement moins de 10 IR3 entièrement caractérisés. Les objectifs de ce travail de thèse étaient (i) d’effectuer une étude épidémiologique des IR3 en France et au Cameroun et (ii) d’étudier les modalités d’expression de l’intégrase et des cassettes de ces intégrons. Nos travaux ont permis d’isoler puis de décrire 3 nouveaux IR3 présents au sein de bactéries environnementales appartenant aux genres Aeromonas, Acinetobacter et Citrobacter. Les cassettes de ces IR3 codent des résistances aux bétalactamines, aminosides et ammoniums quaternaires. De plus, nous avons caractérisé des IR3 dans 3 souches de Delftia spp. (2 D.acidovorans et 1 D. tsuruhatensis) isolées en Afrique ; les cassettes de ces intégrons ne codent pas de résistance aux antibiotiques. L’axe plus fondamental de ce travail de thèse a permis de montrer que le PintI3(1) est le promoteur impliqué dans l’expression du gène intI3. De plus, nous avons montré que les variants du promoteur Pc, ainsi que les variants du promoteur PintI3(1) sont fonctionnels et de force différente. Il ressort de nos travaux que l’environnement constituerait un réservoir d’intégrons de classe 3 et que ces supports génétiques pourraient jouer un rôle important dans la dissémination de la résistance au sein de cet écosystème.
- Published
- 2016
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