1. Methiothepin Increases Chemotherapy Efficacy against Resistant Melanoma Cells.
- Author
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Durand N, Simsir M, Signetti L, Labbal F, Ballotti R, and Mus-Veteau I
- Subjects
- Cell Line, Tumor, Cell Proliferation drug effects, Doxorubicin administration & dosage, Humans, Patched-1 Receptor metabolism, Pyridones administration & dosage, Pyrimidinones administration & dosage, Vemurafenib administration & dosage, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Drug Resistance, Neoplasm drug effects, Melanoma drug therapy, Methiothepin pharmacology, Methiothepin therapeutic use, Skin Neoplasms drug therapy
- Abstract
We previously reported that methiothepin, a small molecule known as a nonselective serotonin 5-HT receptor antagonist, inhibited the doxorubicin efflux activity of the Hedgehog receptor Ptch1 and enhanced the cytotoxic, pro-apoptotic, anti-proliferative, and anti-clonogenic effects of doxorubicin on adrenocortical carcinoma cells. Here, we show that methiothepin also inhibits doxorubicin efflux and increases doxorubicin cytotoxicity in melanoma cells which endogenously overexpress Ptch1. Melanoma patients having the BRAF
V600E mutation are treated with vemurafenib, an inhibitor of BRAFV600E , often in combination with trametinib, an inhibitor of MEK. Almost all patients ultimately acquire resistance to the treatment leading to disease progression. Here, we report that methiothepin overcomes the resistance of BRAFV600E melanoma cells by enhancing the cytotoxicity of vemurafenib and trametinib on these cells leading to melanoma cells death. We observe that the addition of methiothepin to vemurafenib prevents migration of resistant melanoma cells more efficiently than vemurafenib alone. Our results provide an additional proof that Ptch1 drug efflux inhibition increases the effectiveness of anti-cancer treatments and overcomes resistance of melanoma cells expressing Ptch1.- Published
- 2021
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