29 results on '"Martín-Biedma B"'
Search Results
2. Association between xerostomia, oral and general health, and obesity in adults. A cross-sectional pilot study
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Martín-Biedma B, Mario Pérez-Sayáns, Andrés Blanco-Carrión, J. A. Suárez-Quintanilla, Francisco Gómez-García, Pilar Gándara-Vila, Eva María Otero-Rey, and A Pérez-González
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Adult ,Male ,medicine.medical_specialty ,Pilot Projects ,Logistic regression ,Xerostomia ,Tooth mobility ,symbols.namesake ,access ,stomatognathic system ,Internal medicine ,Surveys and Questionnaires ,medicine ,Humans ,Obesity ,General Dentistry ,UNESCO:CIENCIAS MÉDICAS ,Oral Medicine and Pathology ,business.industry ,Research ,Gold standard ,Anthropometry ,medicine.disease ,Dry mouth ,stomatognathic diseases ,Bonferroni correction ,Cross-Sectional Studies ,Otorhinolaryngology ,covid-19 ,disability ,symbols ,Quality of Life ,Surgery ,dental care ,Female ,Analysis of variance ,medicine.symptom ,business - Abstract
Background The objective of this study was to analyse the association between oral and general health variables and obesity indicators with the sensation of dry mouth or xerostomia as evaluated on the Xerostomia Inventory (XI). Material and Methods A total of 354 randomly selected subjects participated in this cross-sectional pilot study and completed an anonymous questionnaire. Anthropometric, clinical, and xerostomic variables were evaluated. Kruskal-Wallis, ANOVA and Bonferroni test were used for multiple comparisons. ROC curves and multinomial logistic regression were used to determine the (OR) risk of xerostomia. Results A total of 30.7 % of respondents reported xerostomia based on XI. The dry mouth question, the XI taken as a “gold standard”, showed a diagnostic sensitivity of 70.37 %, and a specificity of 83.27 % (AUC=0.768, p
- Published
- 2021
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3. Dentin growth after direct pulp capping with the different fractions of plasma rich in growth factors (PRGF) vs. MAT: experimental study in animal model
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Universidad de Sevilla. Departamento de Estomatología, Gaviño-Orduña, J. F., Caviedes-Bucheli, J., Manzanares-Céspedes, M. C., Román-Richón, S., Martín-Biedma, B., Segura Egea, Juan José, López-López, J., Universidad de Sevilla. Departamento de Estomatología, Gaviño-Orduña, J. F., Caviedes-Bucheli, J., Manzanares-Céspedes, M. C., Román-Richón, S., Martín-Biedma, B., Segura Egea, Juan José, and López-López, J.
- Abstract
Background: This study aimed to evaluate the area of dentin growth in rabbit incisors after pulp capping with plasma rich in growth factors (PRGF) compared with mineral trioxide aggregate (MTA) by fluorescence. Methods: twenty-seven upper and lower incisors of rabbits were divided into 4 groups: poor PRGF (F1) (n = 9 teeth), rich PRGF (F2) (n = 8 teeth), ProRoot MTA (positive control, n = 5 teeth), and untreated (NC) (negative control, n = 5). Fluorochrome markers were injected 24 h before surgery and the day before euthanasia, 28 days after the vital pulp therapy (VPT). Two transverse cuts were performed to every tooth: the first cut (A), 1 mm incisal to the gingival margin, and the second cut (B), 5 mm apical to the first cut. The sections were assessed with histomorphometric evaluation by fluorescence microscopy, comparing the dentin area between fluorescence marks and the total mineralized area. Results: The higher percentage of dentin growth was observed in the F2 group (B = 63.25%, A = 36.52%), followed by F1 (B = 57.63%, A = 30,12%) and MTA (B = 38.64%, A = 15.74%). The group with lowest percentage of dentin growth was the NC group (B = 29.22%, A = 7.82%). Significant difference (p < 0.05) was found between F2 group and MTA, also statistically significant difference has been observed comparing dentin growth areas of NC group with F1 and F2 groups. Conclusions: The application of PRGF rich and poor fraction as a pulp capping material stimulated dentin formation more intensively than MTA and NC.
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- 2021
4. Association between xerostomia, oral and general health, and obesity in adults. A cross-sectional pilot study
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Pérez-González, A., primary, Suárez-Quintanilla, JA., additional, Otero-Rey, E., additional, Blanco-Carrión, A., additional, Gómez-García, FJ., additional, Gándara-Vila, P., additional, Martín-Biedma, B., additional, and Pérez-Sayáns, M., additional
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- 2021
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5. Oral candidosis in the older patient
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Otero Rey, E., Peñamaría Mallón, M., Rodríguez Piñón, M., Martín Biedma, B., and Blanco Carrión, A.
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stomatognathic diseases ,older patient ,Oral candidiasis ,oral mucosa ,gerodontology ,mucosa oral ,Candidiasis oral ,paciente mayor ,gerodontología - Abstract
La candidiasis o candidosis oral es la enfermedad infecciosa ocasionada por el crecimiento de las colonias de Cándida y la penetración de las mismas en los tejidos orales cuando las barreras físicas y las defensas del huésped se encuentran alteradas. Es una infección frecuente de la cavidad oral de los adultos de edad avanzada. Aunque la incidencia real se desconoce, se sabe que existe una prevalencia aumentada en ciertas ocasiones como ocurre en ancianos, en presencia de prótesis mucosoportadas, xerostomía o en patologías asociadas frecuentemente en los mayores. Los tipos clínicos más característicos son la forma seudomembranosa y la eritematosa (palatina y lingual). Pueden tener evolución aguda o crónica según la persistencia de los factores predisponentes. También son frecuentes procesos bucales comúnmente asociados: estomatitis protética, queilitis angular, glositis romboidal y lengua vellosa. La mayor parte de las candidiasis orales tienen un diagnóstico clínico, pero ha de confirmarse demostrando la penetración de la cándida en la mucosa oral, siendo el frotis la técnica de elección. Antes de comenzar el tratamiento, debemos estar seguros que se trata de una candidiasis oral, el tipo clínico y los factores predisponentes relacionados con la infección. Empezaremos siempre eliminando estos factores predisponentes, en el adulto mayor, la polifarmacología, la xerostomía, enfermedades crónicas y el uso de prótesis mucosoportadas son situaciones frecuentes que habrá que controlar. Instauraremos medidas higiénicas bucales y posteriormente si es necesario, utilizaremos fármacos antifúngicos, comenzando siempre con formas tópicas. Oral Candidiasis or Candidosis is the infectious disease caused by the growth of colonies of Candida and penetration in the oral tissues when physical barriers and host defenses are altered. It is the most common fungal infection of oral involvement. It is a common infection of the oral cavity in elderly adults. Although the true incidence is unknown, it is known that there is an increased prevalence in certain situations in the elderly: tissue-borne prosthesis, xerostomia or disorders frequently associated. The most characteristic clinical types are pseudomembranous and erythematous (palatal and lingual) form. They may have acute or chronic evolution as the persistence of predisposing factors. They are also frequent mouth commonly associated processes: denture stomatitis, angular cheilitis, rhomboid glossitis and hairy tongue. Most oral candidiasis have a clinical diagnosis, but must be confirmed by demonstrating penetration of candida on the oral mucosa, being the preferred technique smears. Before starting treatment, we must be sure that it is an oral candidiasis, clinical type and predisposing factors associated with infection. Always start eliminating these predisposing factors in the elderly, the polypharmacy, xerostomia, chronic diseases and the use of tissue-borne prostheses are common situations which must be controlled. We will initiate oral hygiene measures and then if necessary, use antifungal drugs, always starting with topical forms.
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- 2015
6. La endodoncia en los pacientes mayores
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Martín Biedma, B., Castelo Baz, P., Otero Rey, E., Ruiz Piñón, M., and Blanco Carrión, A.
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irrigantes ,older patient ,gerodontology ,obturación ,Endodoncia ,irrigants ,obturation ,older patients ,Endodontics - Abstract
La endodoncia en el paciente geriátrico es un procedimiento cada vez más habitual en la clínica odontológica. Debemos, dadas las características médicas y dentales de muchos de estos pacientes mayores, planificar correctamente los procesos instrumentales y prever las posibles dificultades que puedan surgir motivadas por conductos estrechos, cámaras pulpares calcificadas etc. En la instrumentación de estos dientes, someteremos a los instrumentos a alto estrés por torsión, motivado por la amplia superficie de contacto entre el instrumento y las paredes, por lo que será totalmente necesario la realización de una preinstrumentación rotatoria y consideramos que es una buena opción el uso del movimiento recíproco. La preparación de los conductos debe dejar un remanente dentinario suficiente, mantenimiento del foramen apical y conicidad progresiva que facilita la administración de irrigantes a lo largo de la longitud de los conductos y las fuerzas de condensación para la obturación. Como irrigantes utilizamos, de forma general, el hipoclorito de sodio al 5% a lo largo de toda la fase instrumental; y una combinación del hipoclorito con un quelante durante la irrigación preobturación que activaremos con algún dispositivo ultrasónico o de aspiración apical negativa. La obturación la realizaremos con sistemas termoplásticos que nos aportan el mejor sellado tridimensional del sistema de conductos. Endodontics in geriatric patients is an increasingly common procedure in the dental clinic. Medical and dental characteristics of these patients makes us properly plan our procedures to anticipate possible difficulties as narrow ducts, calcifications etc. Our procedures should provide for the possible difficulties that may arise cause by narrow ducts, calcified pulp chambers, etc. During the instrumentation of these teeth, we submit a high stress of torsion to our instruments, motivated by the wide surface of contact between the instrument and the walls. For these reason, it will be absolutely necessary to make a rotary preinstrumentation, then, for the final instrumentation, we must consider the alternating rotation as a good option. The canal preparation should leave one sufficient dentin remnant, maintenance of the apical foramen and the proper taper to facilitate the administration of irrigants along the length of the ducts and the forces of condensation for obturation. As irrigants we use, in general, sodium hypochlorite 5% throughout the entire instrumental phase; and a combination of hypochlorite with a chelator for irrigation preobturation, in this phase we will activate with any ultrasonic device or with apical negative pressure. We will make the obturation with thermoplastic systems that provide us a completely three-dimensional sealing of the root canal system.
- Published
- 2015
7. In vitro comparison of passive and continuous ultrasonic irrigation in simulated lateral canals of extracted teeth
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Pablo Castelo-Baz, Berta Rivas-Mundiña, José González Bahillo, Benjamín Martín-Biedma, Manuel Ruíz-Piñón, Giuseppe Cantatore, Purificación Varela-Patiño, Castelo Baz, P, Martín Biedma, B, Cantatore, Giuseppe, Ruíz Piñón, M, Bahillo, J, Rivas Mundiña, B, and Varela Patiño, P.
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Irrigation ,Materials science ,Time Factors ,Sodium Hypochlorite ,Dentistry ,chemistry.chemical_compound ,Sonication ,Tooth Apex ,Materials Testing ,Pressure ,Humans ,Ultrasonics ,Dental operating microscope ,Coloring Agents ,Therapeutic Irrigation ,General Dentistry ,Edetic Acid ,Microscopy ,Ultrasonic irrigation ,Root Canal Irrigants ,business.industry ,Syringes ,Direct observation ,Penetration (firestop) ,Carbon ,Sodium hypochlorite solution ,chemistry ,Continuous ultrasonic irrigation, passive ultrasonic irrigation, positive pressure irrigation, root canal irrigation, vapor lock ,Needles ,Sodium hypochlorite ,Pulp (tooth) ,Dental Pulp Cavity ,business ,Root Canal Preparation - Abstract
Introduction: Complete endodontic system disinfection requires the removal of vital and necrotic pulp tissue, microorganisms, and toxins. The purpose of this study was to compare the effects of 2 ultrasonic irrigation techniques on the penetration of sodium hypochlorite into the main canal and simulated lateral canals of extracted teeth. Methods: Two simulated lateral canals each were created 2, 4, and 6 mm from the working length in 60 single-rooted teeth (6 canals/ tooth, n = 360). To resemble the clinical situation, a closed system was created in each tooth. The teeth were randomly assigned to 3 experimental irrigation groups: group 1 (n = 20), positive pressure irrigation (PPI); group 2 (n = 20), passive ultrasonic irrigation (PUI); and group 3 (n = 20), continuous ultrasonic irrigation (CUI). Samples were evaluated by direct observation of still images recorded under a dental operating microscope. To examine irrigating solution penetration, 20% Chinese ink (Sanford Rotring GmbH, Hamburg, Germany) was added to a 5% sodium hypochlorite solution and delivered into the root canals. Results: The results showed a significantly higher (P < .05) penetration of irrigant into the lateral canals in the CUI group. PUI and CUI did not differ significantly in solution penetration into the apical thirds of the main canals. The PPI group showed a significantly lower penetration of sodium hypochlorite into the main and lateral canals compared with the CUI and PUI groups. Conclusions: CUI as a final rinse significantly increased the penetration of irrigating solution into
- Published
- 2011
8. Three-Dimensional Printed Teeth in Endodontics: A New Protocol for Microcomputed Tomography Studies.
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Reis T, Barbosa C, Franco M, Silva R, Alves N, Castelo-Baz P, Martín-Cruces J, and Martín-Biedma B
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This study aimed to describe a support material removal protocol (SMRP) from inside the root canals of three-dimensional printed teeth (3DPT) obtained by the microcomputed tomography (microCT) of a natural tooth (NT), evaluate its effectiveness by comparing the 3DPT to NT in terms of internal anatomy and behaviour toward endodontic preparation, and evaluate if 3DPT are adequate to assess the differences between two preparation systems. After the SMRP, twenty 3DPT printed by PolyJet™ were microCT scanned before preparation and thereafter randomly assigned into two groups (n = 10). One group and NT were prepared using ProTaper Gold
® (PTG), and the other group with Endogal® (ENDG). MicroCT scans were carried out after preparation, and the volume increase, volume of dentin removed, centroids, transportation, and unprepared areas were compared. For the parameters evaluated, no significant differences were found between the 3DPT and NT before and after preparation ( p > 0.05), and no significant differences were found between the 3DPT PTG group and the 3DPT ENDG group ( p > 0.05). It can be concluded that the SMRP described is effective in removing the support material SUP706B™. PolyJet™ is adequate for printing 3DPT. Furthermore, 3DPT printed with high-temperature RGD525™ have similar behaviour during endodontic preparation with PTG as the NT, and 3DPT can be used to compare two preparation systems.- Published
- 2024
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9. Cone-Beam Computed Tomography for the Evaluation of Dental Pulp Chamber Volume: Implications for Clinics and Teaching.
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Llacer-Martínez M, Martín-Biedma B, Sanz MT, Aura-Tormos JI, Fos-Galve P, Fernández-Muñiz Z, Vega JA, and Jovani-Sancho M
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The dental pulp chamber volume is a fundamental measurement in the field of endodontics, but also in forensic sciences, teaching and training, or tissue engineering. This study evaluates the precision of cone-beam computed tomography (CBCT) in comparison with computed micro-tomography (micro-CT) in evaluating the pulp chamber volume of the upper central incisors ex vivo. The intra-operator and inter-operator errors were evaluated, and the results for the two techniques were compared with those of a T-test for paired samples. The intra-operator and inter-operator errors were >0.05, indicating adequate reproducibility in each operator and no significant differences between their measurements. On the other hand, no significant differences between the two measurement techniques were found. The present results demonstrate that CBCT is a precise, feasible, and reproducible technique for the evaluation of the dental pulp chamber volume ex vivo. The results provided with this method are useful for different medical domains but also for the teaching and training of undergraduate and postgraduate students. Furthermore, the findings of this study carry significant clinical implications, as the accurate assessment of the pulp chamber volume is critical in the diagnosis and treatment of various endodontic conditions. The ability of CBCT to provide reliable 3D dental anatomy measurements can enhance the planning of endodontic treatments by allowing for a better understanding of the internal tooth morphology. Additionally, the precision and reproducibility of CBCT in assessing the pulp chamber volume can contribute to improved clinical outcomes and reduced complications during endodontic procedures. These findings further support the increasingly vital role of CBCT in modern clinical practice and underscore its value as an indispensable tool in the field of dentistry.
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- 2024
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10. New Technique for Wedge Selection in Direct Class II Restorations: A Pilot Study.
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Gancedo-Gancedo T, Martín-Biedma B, Domínguez-Cachón J, Garrido-Parada S, Ababii V, Pereira-Lores P, García-Varela S, and Castelo-Baz P
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Background: Performing an appropriate Class II direct restoration is a great challenge. The correct selection of the matrix system and the elements used for its stabilization will have a great impact on the result. The aim of this study is to show a new digital method for a predictable selection of the wedge and compare it with the conventional method. Methods: Sixty patients were randomly divided into two groups. In Group 0, the wedge was selected intraoperatively by visual examination, while in Group 1 the wedge was selected preoperatively through the measurement of the cervical embrasure on the bite-wing radiography. The number of wedges used, modifications to them, and tissue damage were registered, along with the quality of the proximal contact and the marginal adaptation. Results: Student's t -test revealed a statistical difference between the number of wedges used, which was higher in Group 0 ( p < 0.05). Pearson Chi-square test showed that tissue damage was statistically higher in Group 0 ( p < 0.05), while there was no statistically significant difference between groups in wedge modifications ( p > 0.05). Group 1 revealed a higher frequency of satisfactory proximal contact and marginal adaptation ( p < 0.05). Conclusions: This new technique reduces wedges waste and tissue damage and provides an adequate interproximal anatomy.
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- 2024
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11. Acid-Sensing Ion Channels' Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body.
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Martínez-Barbero G, García-Mesa Y, Cobo R, Cuendias P, Martín-Biedma B, García-Suárez O, Feito J, Cobo T, and Vega JA
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- Humans, Acid Sensing Ion Channels metabolism, Chemoreceptor Cells metabolism, Peripheral Nervous System metabolism, Carotid Body metabolism, Acidosis metabolism
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The carotid body is a major peripheral chemoreceptor that senses changes in arterial blood oxygen, carbon dioxide, and pH, which is important for the regulation of breathing and cardiovascular function. The mechanisms by which the carotid body senses O
2 and CO2 are well known; conversely, the mechanisms by which it senses pH variations are almost unknown. Here, we used immunohistochemistry to investigate how the human carotid body contributes to the detection of acidosis, analyzing whether it expresses acid-sensing ion channels (ASICs) and determining whether these channels are in the chemosensory glomic cells or in the afferent nerves. In ASIC1, ASIC2, and ASIC3, and to a much lesser extent ASIC4, immunoreactivity was detected in subpopulations of type I glomus cells, as well as in the nerves of the carotid body. In addition, immunoreactivity was found for all ASIC subunits in the neurons of the petrosal and superior cervical sympathetic ganglia, where afferent and efferent neurons are located, respectively, innervating the carotid body. This study reports for the first time the occurrence of ASIC proteins in the human carotid body, demonstrating that they are present in glomus chemosensory cells (ASIC1 < ASIC2 > ASIC3 > ASIC4) and nerves, presumably in both the afferent and efferent neurons supplying the organ. These results suggest that the detection of acidosis by the carotid body can be mediated via the ASIC ion channels present in the type I glomus cells or directly via sensory nerve fibers., Competing Interests: The authors declare no conflict of interest.- Published
- 2023
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12. Author Correction: Functionalization of gutta-percha surfaces with argon and oxygen plasma treatments to enhance adhesiveness.
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Ferreira I, Lopes C, Rodrigues MS, Rodrigues PV, Castro C, Braga AC, Lopes M, Vaz F, Pina-Vaz I, and Martín-Biedma B
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- 2023
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13. Functionalization of gutta-percha surfaces with argon and oxygen plasma treatments to enhance adhesiveness.
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Ferreira I, Lopes C, Rodrigues MS, Rodrigues PV, Castro C, Braga AC, Lopes M, Vaz F, Pina-Vaz I, and Martín-Biedma B
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- Humans, Adhesiveness, Argon, Oxygen, Gutta-Percha, Gases
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Gutta-percha's lack of adhesion has been presented as a drawback to avoid gaps at sealer/gutta-percha interface. Plasma treatments have been scarcely assessed on gutta-percha surfaces as a method of enhancing adhesiveness. This study aimed to evaluate the effect of low-pressure Argon and Oxygen plasma atmospheres on conventional and bioceramic gutta-percha standardized smooth discs, assessing their roughness, surface free energy, chemical structure, and sealer wettability. A Low-Pressure Plasma Cleaner by Diener Electronic (Zepto Model) was used. Different gases (Argon or Oxygen), powers (25 W, or 50 W), and exposure times (30 s, 60 s, 120 s, or 180 s) were tested in control and experimental groups. Kruskal-Wallis and Student's t-test were used in data analysis. Statistically significant differences were detected when P < 0.05. Both gases showed different behaviors according to the parameters selected. Even though chemical changes were detected, the basic molecular structure was maintained. Argon or Oxygen plasma treatments favoured the wetting of conventional and bioceramic gutta-perchas by Endoresin and AH Plus Bioceramic sealers (P < 0.001). Overall, the functionalization of gutta-percha surfaces with Argon or Oxygen plasma treatments can increase roughness, surface free energy and wettability, which might improve its adhesive properties when compared to non-treated gutta-percha., (© 2023. The Author(s).)
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- 2023
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14. Pacinian Corpuscles as a Diagnostic Clue of Ledderhose Disease-A Case Report and Mapping of Pacinian Corpuscles of the Sole.
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Feito J, Esteban R, García-Martínez ML, García-Alonso FJ, Rodríguez-Martín R, Rivas-Marcos MB, Cobo JL, Martín-Biedma B, Lahoz M, and Vega JA
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Background: Plantar fibromatosis, known as Ledderhose disease, is a neoplastic disease characterized by a locally-aggressive bland fibroblastic proliferation. Although Pacinian corpuscles alterations are commonly described in palmar fibromatosis, there are still no references about Pacinian corpuscles alterations in the rarer plantar version., Methods: We present a case report where a wide cutaneous resection, including the plantar fascia was performed, allowing a detailed study of Pacinian corpuscles. Pacinian corpuscles were analyzed using immunohistochemistry for neurofilament proteins, S100 protein, CD34, vimentin, glucose transporter 1, epithelial membrane antigen, neural-cell adhesion molecule, actin, desmin, type IV collagen, and high-affinity neurotrophin Trk-receptors. Moreover, the density and the size of the corpuscles were determined., Results: A clear increase in the number (hyperplasia) of Pacinian corpuscles was evidenced in the Ledderhose disease plantar fascia in comparison with similarly aged normal subjects. Pacinian hypertrophy was not demonstrated, but a significant decrease in the number of corpuscular lamellae was noted, with a subsequent increase in the interlamellar spaces. Pacinian corpuscles from the pathological plantar fascia showed an abnormal structure and immunohistochemical profile, generally without identifiable axons, and also absence of an inner core or an intermediate layer. Moreover, other molecules related with trophic maintenance of corpuscles were also absent. Finally, a vascular proliferation was commonly noted in some corpuscles, which involved all corpuscular constituents., Conclusion: The observed Pacinian corpuscles hyperplasia could be considered a diagnostic clue of plantar fibromatosis.
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- 2022
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15. Effect of Rotational Speed on the Resistance of NiTi Alloy Endodontic Rotary Files to Cyclic Fatigue-An In Vitro Study.
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Faus-Matoses V, Faus-Llácer V, Ruiz-Sánchez C, Jaramillo-Vásconez S, Faus-Matoses I, Martín-Biedma B, and Zubizarreta-Macho Á
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The present study aims to evaluate and contrast the function of the rotational speed of NiTi alloy endodontic rotary files on how resistant they are to dynamic cyclic fatigue. Methods: A total of 150 NiTi alloy endodontic rotary files with similar geometrical design and metallurgical properties were randomly divided into study groups: Group A: 200 rpm (n = 30); Group B: 350 rpm (n = 30); Group C: 500 rpm (n = 30); Group D: reciprocating movement at 350 rpm with 120° counterclockwise and 30° clockwise motion (350 rpm+) (n = 30); and Group E: reciprocating movement at 400 rpm with 120° counterclockwise and 30° clockwise motion (400 rpm+) (n = 30). A dynamic device was designed to carry out dynamic cyclic fatigue tests using artificial root canal systems made from stainless steel with an apical diameter of 250 µm, 5 mm radius of curvature, 60° curvature angle, and 6% taper, and 20 mm in length. A Weibull statistical analysis and ANOVA test were used to analyze the results. Results: The ANOVA analysis showed differences in time to failure among all the study groups that were of statistical significance (p < 0.001). Conclusions: NiTi alloy endodontic rotary files using reciprocating movement at 350 rpm with 120° counterclockwise and 30° clockwise motion exhibit greater resistance to dynamic cyclic fatigue than files used with a reciprocating movement at 400 rpm with 120° counterclockwise and 30° clockwise motion, continuous rotational speed at 200 rpm, continuous rotational speed at 350 rpm, or continuous rotational speed at 500 rpm; it is therefore advisable to use reciprocating movements at a low speed.
- Published
- 2022
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16. Merkel Cell Carcinoma Display PIEZO2 Immunoreactivity.
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García-Mesa Y, Martín-Sanz R, García-Piqueras J, Cobo R, Muñoz-Bravo S, García-Suárez O, Martín-Biedma B, Vega JA, and Feito J
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As an essential component of mechano-gated ion channels, critically required for mechanotransduction in mammalian cells, PIEZO2 is known to be characteristically expressed by Merkel cells in human skin. Here, we immunohistochemically investigated the occurrence of Piezo channels in a case series of Merkel cell carcinoma. A panel of antibodies was used to characterize Merkel cells, and to detect PIEZO2 expression. All analyzed tumors displayed PIEZO2 in nearly all cells, showing two patterns of immunostaining: membranous and perinuclear dot-like. PIEZO2 co-localized with cytokeratin 20, chromogranin A, synaptophysin and neurofilament. Moreover, neurofilament immunoreactive structures resembling nerve-Merkel cell contacts were occasionally found. PIEZO2 was also detected in cells of the sweat ducts. The role of PIEZO2 in Merkel cell carcinoma is still unknown, but it could be related with the mechanical regulation of the tumor biology or be a mere vestige of the Merkel cell derivation.
- Published
- 2022
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17. Endodontic Rotary Files, What Should an Endodontist Know?
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Dablanca-Blanco AB, Castelo-Baz P, Miguéns-Vila R, Álvarez-Novoa P, and Martín-Biedma B
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- Equipment Design, Humans, Titanium, Endodontists, Root Canal Preparation
- Abstract
Clinicians should be aware of all the characteristics and capacities of the instruments that are possible to use when conducting a root canal treatment. The wide variety of nickel-titanium (Ni-Ti) rotary systems on the market and the lack of standardisation of this type of instrument makes the choice in each specific case difficult. Therefore, this review is intended to summarize the characteristics that should be taken into account when choosing one instrument over another. It will be essential to know characteristics, of alloy from which the instrument is made. Moreover, the geometry of the instrument will determine its behaviour, being the mass, the one that marks its resistance to a greater extent. The movement performed by the file is another of the fundamental keys to understand rotary instruments. In conclusion, when performing root canal treatment, the characteristics of the instrument and the tooth must be taken into account, and the operator's own limitations should be known. This paper provides the key points to keep in mind when making this type of treatment.
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- 2022
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18. Effectiveness of Different Irrigant Activation Systems on Smear Layer Removal: A Scanning Electron Microscopic Study.
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Miguéns-Vila R, Martín-Biedma B, Aboy-Pazos S, Uroz-Torres D, Álvarez-Nóvoa P, Dablanca-Blanco AB, Varela-Aneiros I, and Castelo-Baz P
- Abstract
The aim of this study was to evaluate the effectiveness of smear layer removal after the use of different irrigation methods (passive ultrasonic irrigation (PUI), continuous ultrasonic irrigation (CUI), apical negative pressure irrigation and conventional irrigation) using scanning electron microscopy (SEM) as an analytical tool. A total of 100 single-canal teeth were decoronated and randomly divided into five groups ( n = 20) according to the irrigation method used: conventional irrigation with front outlet syringe, conventional irrigation with lateral outlet syringe, apical negative pressure irrigation (EndoVac), PUI with Irrisafe and CUI with ProUltra PiezoFlow ultrasonic irrigation needle. Root canal preparation was performed with the ProTaper Gold system up to the F4 instrument, and 5.25% NaOCl was used as an irrigant. After chemical-mechanical preparation, the roots were split longitudinally, and the coronal, middle and apical thirds were examined. SEM digital photomicrographs were taken at ×1000 magnification to evaluate the amount of smear layer in each root canal third; CUI significantly removed more smear layer than any other irrigant activation protocol ( p < 0.05); CUI was more effective in removing the smear layer than the other irrigation protocols. However, none of the irrigation protocols were able to produce root canals completely free from smear layer.
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- 2022
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19. In-Silico Detection of Oral Prokaryotic Species With Highly Similar 16S rRNA Sequence Segments Using Different Primer Pairs.
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Regueira-Iglesias A, Vázquez-González L, Balsa-Castro C, Blanco-Pintos T, Martín-Biedma B, Arce VM, Carreira MJ, and Tomás I
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- Archaea genetics, High-Throughput Nucleotide Sequencing methods, Humans, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA methods, Microbiota genetics
- Abstract
Although clustering by operational taxonomic units (OTUs) is widely used in the oral microbial literature, no research has specifically evaluated the extent of the limitations of this sequence clustering-based method in the oral microbiome. Consequently, our objectives were to: 1) evaluate in-silico the coverage of a set of previously selected primer pairs to detect oral species having 16S rRNA sequence segments with ≥97% similarity; 2) describe oral species with highly similar sequence segments and determine whether they belong to distinct genera or other higher taxonomic ranks. Thirty-nine primer pairs were employed to obtain the in-silico amplicons from the complete genomes of 186 bacterial and 135 archaeal species. Each fasta file for the same primer pair was inserted as subject and query in BLASTN for obtaining the similarity percentage between amplicons belonging to different oral species. Amplicons with 100% alignment coverage of the query sequences and with an amplicon similarity value ≥97% (ASI97) were selected. For each primer, the species coverage with no ASI97 (SC-NASI97) was calculated. Based on the SC-NASI97 parameter, the best primer pairs were OP_F053-KP_R020 for bacteria (region V1-V3; primer pair position for Escherichia coli J01859.1: 9-356); KP_F018-KP_R002 for archaea (V4; undefined-532); and OP_F114-KP_R031 for both (V3-V5; 340-801). Around 80% of the oral-bacteria and oral-archaea species analyzed had an ASI97 with at least one other species. These very similar species play different roles in the oral microbiota and belong to bacterial genera such as Campylobacter , Rothia , Streptococcus and Tannerella , and archaeal genera such as Halovivax , Methanosarcina and Methanosalsum . Moreover, ~20% and ~30% of these two-by-two similarity relationships were established between species from different bacterial and archaeal genera, respectively. Even taxa from distinct families, orders, and classes could be grouped in the same possible OTU. Consequently, regardless of the primer pair used, sequence clustering with a 97% similarity provides an inaccurate description of oral-bacterial and oral-archaeal species, which can greatly affect microbial diversity parameters. As a result, OTU clustering conditions the credibility of associations between some oral species and certain health and disease conditions. This significantly limits the comparability of the microbial diversity findings reported in oral microbiome literature., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Regueira-Iglesias, Vázquez-González, Balsa-Castro, Blanco-Pintos, Martín-Biedma, Arce, Carreira and Tomás.)
- Published
- 2022
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20. Efficacy of continuous apical negative ultrasonic irrigation (CANUI) in penetration of simulated lateral canals in extracted teeth.
- Author
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Castelo-Baz P, Lozano FJR, Ginzo-Villamayor MJ, Vila RM, Seoane-Romero J, Martín-Cruces J, and Martín-Biedma B
- Subjects
- Humans, Materials Testing, Root Canal Preparation methods, Therapeutic Irrigation instrumentation, Tooth Extraction, Ultrasonics, Root Canal Irrigants administration & dosage, Root Canal Preparation instrumentation, Sodium Hypochlorite administration & dosage, Tooth diagnostic imaging
- Abstract
The aim of this study was to evaluate the efficacy of continuous apical negative ultrasonic irrigation into simulated lateral canals and the apical third in straight and curved root canals. Two simulated lateral canals were created 2, 4 and 6 mm from the working length in 120 single-rooted teeth (6 canals/tooth, n = 360 straight, n = 360 curved). The teeth were randomly divided into 3 experimental groups: positive pressure irrigation (PPI) (n = 20); passive ultrasonic irrigation (PUI) (n = 20); continuous apical negative ultrasonic irrigation (CANUI) (n = 20). 20% Chinese ink was added to a 5% sodium hypochlorite solution and delivered into the root canals. The results showed a significantly higher (P < 0.05) penetration of irrigant into the lateral canals and up to working length in the CANUI group for straight and curved roots. CANUI improves penetration into the lateral canals and up to the working length of the cleared teeth in straight and curved roots.
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- 2021
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21. Ageing of the somatosensory system at the periphery: age-related changes in cutaneous mechanoreceptors.
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García-Piqueras J, García-Mesa Y, Cárcaba L, Feito J, Torres-Parejo I, Martín-Biedma B, Cobo J, García-Suárez O, and Vega JA
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Fingers innervation, Humans, Male, Mechanoreceptors pathology, Middle Aged, Skin innervation, Young Adult, Aging pathology, Merkel Cells pathology, Pacinian Corpuscles pathology, Touch physiology
- Abstract
Decline of tactile sensation associated with ageing depends on modifications in skin and both central and peripheral nervous systems. At present, age-related changes in the periphery of the somatosensory system, particularly concerning the effects on mechanoreceptors, remain unknown. Here we used immunohistochemistry to analyse the age-dependent changes in Meissner's and Pacinian corpuscles as well as in Merkel cell-neurite complexes. Moreover, variations in the neurotrophic TrkB-BDNF system and the mechanoprotein Piezo2 (involved in maintenance of cutaneous mechanoreceptors and light touch, respectively) were evaluated. The number of Meissner's corpuscles and Merkel cells decreased progressively with ageing. Meissner's corpuscles were smaller, rounded in morphology and located deeper in the dermis, and signs of corpuscular denervation were found in the oldest subjects. Pacinian corpuscles generally showed no relevant age-related alterations. Reduced expression of Piezo2 in the axon of Meissner's corpuscles and in Merkel cells was observed in old subjects, as well was a decline in the BDNF-TrkB neurotrophic system. This study demonstrates that cutaneous Meissner's corpuscles and Merkel cell-neurite complexes (and less evidently Pacinian corpuscles) undergo morphological and size changes during the ageing process, as well as a reduction in terms of density. Furthermore, the mechanoprotein Piezo2 and the neurotrophic TrkB-BDNF system are reduced in aged corpuscles. Taken together, these alterations might explain part of the impairment of the somatosensory system associated with ageing., (© 2019 Anatomical Society.)
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- 2019
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22. Fracture and deformation of ProTaper Next instruments after clinical use.
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Fernández-Pazos G, Martín-Biedma B, Varela-Patiño P, Ruíz-Piñón M, and Castelo-Baz P
- Abstract
Background: The aim of this study was to evaluate the fracture and defects observed in ProTaper Next files discarded after a normal clinical use., Material and Methods: 571 ProTaper Next rotary instruments were collected after clinical use from the clinic of endodontics over 12 months. The length of the files was measured using a digital caliper to determine any fracture, later all the files were evaluated under a stereomicroscope to observe defects such as unwinding, curving or fracture. The data obtained were analyzed using a chi-square and z test., Results: 13.83% of the discarded files showed defects, the most frequent defect was fracture (7.53%). The highest rate of fracture was observed in the X1 (17.04) files (19.87%). The presence of deformations without fracture was also more frequent in the 17.04 file (11.8%)., Conclusions: Because of the relatively high incidence of deformation of smaller files, these instruments should be considered as a single use. It is important not to exceed the maximum uses recommended by the manufacturer to reduce the risk of cyclic fatigue, the main cause of fracture of the files (79.07%). It is also important to observe each file after use to discard small defects or fractures. Key words: Clinical use, deformation, fracture, M-Wire, ProTaper Next., Competing Interests: Conflict of interest statement: The authors have declared that no conflict of interest exist.
- Published
- 2018
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23. Use of antibiotics by spanish dentists receiving postgraduate training in endodontics.
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Alonso-Ezpeleta O, Martín-Jiménez M, Martín-Biedma B, López-López J, Forner-Navarro L, Martín-González J, Montero-Miralles P, Jiménez-Sánchez MDC, Velasco-Ortega E, and Segura-Egea JJ
- Abstract
Background: The incidence of endodontic infections is high. The contribution of Endodontics to the global problem of antibiotic resistance could be significant. The ESE, together with the World Health Organization, are promoting the World Antibiotic Awareness Week (13-19 November 2017) to promote the appropriate use of systemic antibiotics in Endodontics. The objective of this study was to determine the prescription pattern of antibiotics in the treatment of endodontic infections of Spanish dentists attending specialization programs in Endodontics., Material and Methods: Dentists from five Spanish endodontic postgraduate programs were requested to answer a one-page questionnaire surveying about antibiotics indications. Seventy-three dentists were required to participate in this investigation, and 67 (91.2%) fulfilled satisfactorily the survey and were included in the study. Data were analyzed using descriptive statistics and chi square test., Results: The average duration of antibiotic therapy was 6.8±1.2 days. All respondents chose amoxicillin as first choice antibiotic in patients with no medical allergies, alone (40%) or associated to clavulanic acid (60%). The first drug of choice for penicillin allergic patients was clindamycin (72%). For cases of irreversible pulpitis, 22% of respondents prescribed antibiotics. For the scenario of a necrotic pulp, symptomatic apical periodontitis and no swelling, 37% prescribed antibiotics. A quarter of dentists prescribed antibiotics for necrotic pulps with asymptomatic apical periodontitis and a sinus tract., Conclusions: The results of this study show that postgraduate training in Endodontics provides greater awareness of the correct indications of antibiotics. Dentists who have received specialized training in Endodontics have a prescription pattern of antibiotics more adjusted to the guidelines recommended by international organizations and by scientific societies. Key words: Antibiotics, apical periodontitis, dental curriculum, endodontic infections, postgraduate endodontic training., Competing Interests: Conflict of interest statement: Authors have no conflict of interest to declare.
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- 2018
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24. Vertical Root Fracture initiation in curved roots after root canal preparation: A dentinal micro-crack analysis with LED transillumination.
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Miguéns-Vila R, Martín-Biedma B, Varela-Patiño P, Ruíz-Piñón M, and Castelo-Baz P
- Abstract
Background: One of the causative factors of root defects is the increased friction produced by rotary instrumentation. A high canal curvature may increase stress, making the tooth more susceptible to dentinal cracks. The purpose of this study was to evaluate dentinal micro-crack formation with the ProTaper NEXT and ProTaper Universal systems using LED transillumination, and to analyze the micro-crack generated at the point of maximum canal curvature., Material and Methods: 60 human mandibular premolars with curvatures between 30-49° and radii between 2-4 mm were used. The root canals were instrumented using the Protaper Universal® and Protaper NEXT® systems, with the aid of the Proglider® system. The obtained samples were sectioned transversely before subsequent analysis with LED transillumination at 2 mm and 8 mm from the apex and at the point of maximum canal curvature. Defects were scored: 0 for no defects; and 1 for micro-cracks., Results: Root defects were not observed in the control group. The ProTaper NEXT system caused fewer defects (16.7%) than the ProTaper Universal system (40%) ( P <0.05). The ProTaper Universal system caused significantly more micro-cracks at the point of maximum canal curvature than the ProTaper NEXT system ( P <0.05)., Conclusions: Rotary instrumentation systems often generate root defects, but the ProTaper NEXT system generated fewer dentinal defects than the ProTaper Universal system. A higher prevalence of defects was found at the point of maximum curvature in the ProTaper Universal group. Key words: Curved root, Micro-crack, point of maximum canal curvature, ProTaper NEXT, ProTaper Universal, Vertical root fracture., Competing Interests: Conflict of interest statement:The authors declare that no competing interests exist.
- Published
- 2017
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25. Management of large class II lesions in molars: how to restore and when to perform surgical crown lengthening?
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Dablanca-Blanco AB, Blanco-Carrión J, Martín-Biedma B, Varela-Patiño P, Bello-Castro A, and Castelo-Baz P
- Abstract
The restoration of endodontic tooth is always a challenge for the clinician, not only due to excessive loss of tooth structure but also invasion of the biological width due to large decayed lesions. In this paper, the 7 most common clinical scenarios in molars with class II lesions ever deeper were examined. This includes both the type of restoration (direct or indirect) and the management of the cavity margin, such as the need for deep margin elevation (DME) or crown lengthening. It is necessary to have the DME when the healthy tooth remnant is in the sulcus or at the epithelium level. For caries that reaches the connective tissue or the bone crest, crown lengthening is required. Endocrowns are a good treatment option in the endodontically treated tooth when the loss of structure is advanced., Competing Interests: Conflict of Interest: No potential conflict of interest relevant to this article was reported.
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- 2017
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26. Alternating versus continuous rotation: Root canal transportation and centering ratio with the ProTaper Next.
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González-Chapela J, Castelo-Baz P, Varela-Patiño P, Martín-Biedma B, and Ruíz-Piñón M
- Abstract
Background: The technique of alternating rotation has been used with NiTi rotary instruments to increase the resistance to fracture compared with conventional continuous rotation. However, it is still not clear what type of instrumentation could provide better results in the preservation of the original canal anatomy., Aim: The aim of this study is to determine the influence of the type of rotation on canal transportation and centering ability using cone-beam computed tomography (CBCT) imaging., Materials and Methods: In total, 50 mesial canals of mandibular molars with curvatures between 30° and 60° were divided into two experimental groups ( n = 25 each) according to the type of movement employed with the ProTaper Next (PTN) instruments: Group A (alternating rotation) and Group B (continuous rotation). Canals were scanned before and after instrumentation using a CBCT scanner to evaluate root canal transportation and the centering ratio at 3, 5, and 7 mm from the apex. Data were analyzed statistically using Student's t -test; the significance level was set at P < 0.05., Results: There was no significant difference between the groups in canal transportation or the centering ratio at any of the three studied cross sections (3, 5, and 7 mm)., Conclusions: PTN instruments promoted minimal apical transportation and remained relatively centered within the root canal, with no significant difference between alternating and continuous rotation., Competing Interests: There are no conflicts of interest.
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- 2017
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27. Continuous Apical Negative-Pressure Ultrasonic Irrigation (CANUI): A new concept for activating irrigants.
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Castelo-Baz P, Varela-Patiño P, Ruíz-Piñón M, Abella F, Miguéns-Vila R, and Martín-Biedma B
- Abstract
Background: Irrigation of the root canal system is an essential step in the endodontic treatment. The aim of this article is to introduce continuous apical negative-pressure ultrasonic irrigation (CANUI), a new irrigation concept, and compare the characteristics of this new technique with current devices for activating the irrigant., Material and Methods: CANUI is designed for cleaning and disinfecting the root canal system. The device consists of a tube inside another tube that allows the continuous ultrasonic exchange of fresh irrigant, as the irrigant is simultaneously aspirated apically. The coronal and apical tubes are 0.75 and 0.3 mm in diameter, respectively. It is composed of a nickel-titanium microcannula suitable for the working length of curved canals., Results: The CANUI technique has the advantages of apical negative pressure (to avoid apical extrusion of the irrigant) and continuous ultrasonic irrigation (continuous refreshment of the irrigant and forced introduction into the canal ramifications)., Conclusions: The CANUI technique could improve the irrigation technique of dentists to reach more disinfection in endodontic treatments. Key words: Apical negative pressure, continuous ultrasonic irrigation, irrigation in curved canals, irrigation systems., Competing Interests: Conflict of interest statement:The authors deny any conflict of interest.
- Published
- 2017
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28. In vitro comparison of passive and continuous ultrasonic irrigation in curved root canals.
- Author
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Castelo-Baz P, Varela-Patiño P, Cantatore G, Domínguez-Perez A, Ruíz-Piñón M, Miguéns-Vila R, and Martín-Biedma B
- Abstract
Background: The efficacy of endodontic irrigation procedures can be compromised by the complexity of the root canal system. Delivering irrigants to the apical third of curved canals presents a particular challenge to endodontists. This study compared the effects of two ultrasonic irrigation techniques on the penetration of sodium hypochlorite into the main canal and simulated lateral canals of curved roots in extracted teeth., Material and Methods: Two sets of simulated lateral canals were created at 2, 4, and 6 mm from the working length in 60 single-rooted teeth (6 canals/tooth, n = 360 canals). The teeth were randomly divided into three experimental irrigation groups: group 1 (n = 20), positive pressure irrigation (PPI); group 2 (n = 20), passive ultrasonic irrigation (PUI); and group 3 (n = 20), continuous ultrasonic irrigation (CUI). To assess the irrigation solution penetration, 20% Chinese ink (Sanford Rotring GmbH, Hamburg, Germany) was added to a 5% sodium hypochlorite solution and delivered into the curved root canals. The penetration of contrast solution into the simulated lateral canals was scored by counting the number of lateral canals (0-2) penetrated to at least 50% of the total length., Results: The CUI group showed significantly higher ( P < 0.05) irrigant penetration into the lateral canals and into the apical third of the main canals. The PPI group showed significantly lower sodium hypochlorite penetration ( P < 0.001) into the main and lateral canals compared with that in the CUI and PUI groups. Significantly higher irrigant penetration was observed in the PUI group than the PPI group., Conclusions: Using CUI as the final rinse significantly increased the penetration of irrigant solution into the simulated lateral canals and apical third of curved roots. Key words: Continuous ultrasonic irrigation, curved root canals, passive ultrasonic irrigation, positive pressure irrigation, root canal irrigation., Competing Interests: The authors deny any confict of interest.
- Published
- 2016
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29. Unusual root morphology in second mandibular molar with a radix entomolaris, and comparison between cone-beam computed tomography and digital periapical radiography: a case report.
- Author
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López-Rosales E, Castelo-Baz P, De Moor R, Ruíz-Piñón M, Martín-Biedma B, and Varela-Patiño P
- Subjects
- Humans, Male, Mandible diagnostic imaging, Mandibular Nerve, Middle Aged, Molar anatomy & histology, Molar diagnostic imaging, Root Canal Therapy methods, Tooth Abnormalities diagnostic imaging, Tooth Root anatomy & histology, Tooth Root diagnostic imaging, Treatment Outcome, Cone-Beam Computed Tomography, Mandible pathology, Molar pathology, Nerve Block methods, Radiographic Image Enhancement, Tooth Abnormalities pathology, Tooth Root pathology
- Abstract
Introduction: Radix entomolaris presents with an unusual morphology and is a rare occurrence. It is mainly observed in mandibular first molars. The incidence varies in different populations but it is far from common. This is especially true for mandibular second molars which possess the lowest prevalence. Some case reports have shown the presence of this finding in mandibular second molars; however, cases of patients of a white background have not been reported., Case Presentation: The diagnosis and treatment of an infected radix entomolaris in a mandibular second molar in a 45-year-old white man is presented. The diagnosis was made with standard endodontic techniques. Conventional radiographic imaging was augmented with cone-beam computed tomography scans and three-dimensional images which were constructed with dedicated software. The endodontic treatment was done using accepted endodontic procedures. Clinical and radiographic evidence of healing was seen after a 14-month follow-up., Conclusions: The implications of complex and unpredictable root anatomy are discussed in this report. The clinician should consider the possibility of encountering a mandibular second molar with a radix entomolaris. Cone-beam computed tomography is a useful tool in the diagnosis and improvement of root canal therapy.
- Published
- 2015
- Full Text
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