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2. Women Skin Microbiota Modifications during Pregnancy

Author

Radocchia, Giulia, primary, Brunetti, Francesca, additional, Marazzato, Massimiliano, additional, Totino, Valentina, additional, Neroni, Bruna, additional, Bonfiglio, Giulia, additional, Conte, Antonietta Lucia, additional, Pantanella, Fabrizio, additional, Ciolli, Paola, additional, and Schippa, Serena, additional

Published
2024
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6. Poliprotect vs Omeprazole in the relief of heartburn, epigastric pain and burning in patients without erosive esophagitis and gastro-duodenal lesions: A Randomized, Controlled Trial

Author

Corazziari, Enrico Stefano, Gasbarrini, Antonio, D'Alba, Lucia, D'Ovidio, Valeria, Riggio, Oliviero, Passaretti, Sandro, Annibale, Bruno, Cicala, Michele, Repici, Alessandro, Bassotti, Gabrio, Ciacci, Carolina, Di Sabatino, Antonio, Neri, Matteo, Bragazzi, Maria Consiglia, Ribichini, Emanuela, Radocchia, Giulia, Iovino, Paola, Marazzato, Massimiliano, Schippa, Serena, Badiali, Danilo, Gasbarrini, Antonio (ORCID:0000-0002-7278-4823), Corazziari, Enrico Stefano, Gasbarrini, Antonio, D'Alba, Lucia, D'Ovidio, Valeria, Riggio, Oliviero, Passaretti, Sandro, Annibale, Bruno, Cicala, Michele, Repici, Alessandro, Bassotti, Gabrio, Ciacci, Carolina, Di Sabatino, Antonio, Neri, Matteo, Bragazzi, Maria Consiglia, Ribichini, Emanuela, Radocchia, Giulia, Iovino, Paola, Marazzato, Massimiliano, Schippa, Serena, Badiali, Danilo, and Gasbarrini, Antonio (ORCID:0000-0002-7278-4823)

Abstract

Background: In the treatment of upper GI endoscopy-negative patients with heartburn and epigastric pain or burning, antacids, anti-reflux agents, and mucosal protective agents (MPA) are widely used, alone or as add-on treatment to increase response to proton pump inhibitors, which are not indicated in infancy and pregnancy, and account for significant cost expenditure. Aims methods: In this randomized, controlled, double-blind, double-dummy, multicenter trial assessing the efficacy and safety of MPA Poliprotect (neoBianacid®) versus Omeprazole in the relief of heartburn and epigastric pain/burning, 275 endoscopy-negative outpatients were given a 4-week treatment with Omeprazole (20 mg q.d.) or Poliprotect (5 times a day for the initial 2 weeks, and on-demand thereafter), followed by an open-label 4-week treatment period with Poliprotect on demand. Gut microbiota change was assessed. Results: A 2-week treatment with Poliprotect proved non-inferior to Omeprazole for symptom relief (between-group difference in the change in VAS symptom score: [mean, 95% CI] -5.4, -9.9 to -0.1; -6.2, -10.8 to -1.6; ITT and PP populations, respectively).Poliprotect's benefit remained unaltered after shifting to on-demand intake, with no gut microbiota variation. The initial benefit of Omeprazole was maintained against significantly higher use of rescue medicine sachets (mean, 95% CI: Poliprotect 3.9, 2.8-5.0; Omeprazole 8.2, 4.8-11.6) and associated with an increased abundance of oral cavity genera in the intestinal microbiota. No relevant adverse events were reported in either treatment arm. Conclusions: Poliprotect proved non-inferior to standard-dose Omeprazole in symptomatic patients with heartburn/epigastric burning without erosive esophagitis and gastro-duodenal lesions. Gut microbiota was not affected by Poliprotect treatment. The study is registered in Clinicaltrial.gov (NCT03238534) and the EudraCT Database (2015-005216-15).

Published
2023

7. Exploiting Bacteria for Improving Hypoxemia of COVID-19 Patients

Author

Trinchieri, Vito, primary, Marazzato, Massimiliano, additional, Ceccarelli, Giancarlo, additional, Lombardi, Francesca, additional, Piccirilli, Alessandra, additional, Santinelli, Letizia, additional, Maddaloni, Luca, additional, Vassalini, Paolo, additional, Mastroianni, Claudio Maria, additional, and d’Ettorre, Gabriella, additional

Published
2022
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8. Gut Microbiota Structure and Metabolites, Before and After Treatment in Early Rheumatoid Arthritis Patients: A Pilot Study

Author

Marazzato, Massimiliano, primary, Iannuccelli, Cristina, additional, Guzzo, Maria Paola, additional, Nencioni, Lucia, additional, Lucchino, Bruno, additional, Radocchia, Giulia, additional, Gioia, Chiara, additional, Bonfiglio, Giulia, additional, Neroni, Bruna, additional, Guerrieri, Francesca, additional, Pantanella, Fabrizio, additional, Garzoli, Stefania, additional, Vomero, Marta, additional, Barbati, Cristiana, additional, Di Franco, Manuela, additional, and Schippa, Serena, additional

Published
2022
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9. Genetic Diversity of Antimicrobial Resistance and Key Virulence Features in Two Extensively Drug-Resistant Acinetobacter baumannii Isolates

Author

Marazzato, Massimiliano, primary, Scribano, Daniela, additional, Sarshar, Meysam, additional, Brunetti, Francesca, additional, Fillo, Silvia, additional, Fortunato, Antonella, additional, Lista, Florigio, additional, Palamara, Anna Teresa, additional, Zagaglia, Carlo, additional, and Ambrosi, Cecilia, additional

Published
2022
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11. Oral Bacteriotherapy Reduces the Occurrence of Chronic Fatigue in COVID-19 Patients

Author

Santinelli, Letizia, primary, Laghi, Luca, additional, Innocenti, Giuseppe Pietro, additional, Pinacchio, Claudia, additional, Vassalini, Paolo, additional, Celani, Luigi, additional, Lazzaro, Alessandro, additional, Borrazzo, Cristian, additional, Marazzato, Massimiliano, additional, Tarsitani, Lorenzo, additional, Koukopoulos, Alexia E., additional, Mastroianni, Claudio M., additional, d'Ettorre, Gabriella, additional, and Ceccarelli, Giancarlo, additional

Published
2022
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12. Chronic Intestinal Pseudo-Obstruction: Is There a Connection with Gut Microbiota?

Author

Radocchia, Giulia, primary, Neroni, Bruna, additional, Marazzato, Massimiliano, additional, Capuzzo, Elena, additional, Zuccari, Simone, additional, Pantanella, Fabrizio, additional, Zenzeri, Letizia, additional, Evangelisti, Melania, additional, Vassallo, Francesca, additional, Parisi, Pasquale, additional, Di Nardo, Giovanni, additional, and Schippa, Serena, additional

Published
2021
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13. Oxygen Sparing Effect of Bacteriotherapy in COVID-19

Author

Ceccarelli, Giancarlo, primary, Marazzato, Massimiliano, additional, Celani, Luigi, additional, Lombardi, Francesca, additional, Piccirilli, Alessandra, additional, Mancone, Massimo, additional, Trinchieri, Vito, additional, Pugliese, Francesco, additional, Mastroianni, Claudio M., additional, and d’Ettorre, Gabriella, additional

Published
2021
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18. Nanoemulsions of Satureja montana Essential Oil: Antimicrobial and Antibiofilm Activity against Avian Escherichia coli Strains

Author

Rinaldi, Federica, primary, Maurizi, Linda, additional, Conte, Antonietta Lucia, additional, Marazzato, Massimiliano, additional, Maccelli, Alessandro, additional, Crestoni, Maria Elisa, additional, Hanieh, Patrizia Nadia, additional, Forte, Jacopo, additional, Conte, Maria Pia, additional, Zagaglia, Carlo, additional, Longhi, Catia, additional, Marianecci, Carlotta, additional, Ammendolia, Maria Grazia, additional, and Carafa, Maria, additional

Published
2021
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19. Oral Bacteriotherapy in Patients With COVID-19: A Retrospective Cohort Study

Author

Ceccarelli, Giancarlo, primary, Borrazzo, Cristian, additional, Pinacchio, Claudia, additional, Santinelli, Letizia, additional, Innocenti, Giuseppe Pietro, additional, Cavallari, Eugenio Nelson, additional, Celani, Luigi, additional, Marazzato, Massimiliano, additional, Alessandri, Francesco, additional, Ruberto, Franco, additional, Pugliese, Francesco, additional, Venditti, Mario, additional, Mastroianni, Claudio M., additional, and d'Ettorre, Gabriella, additional

Published
2021
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20. 16S Metagenomics Reveals Dysbiosis of Nasal Core Microbiota in Children With Chronic Nasal Inflammation: Role of Adenoid Hypertrophy and Allergic Rhinitis

Author

Marazzato, Massimiliano, primary, Zicari, Anna Maria, additional, Aleandri, Marta, additional, Conte, Antonietta Lucia, additional, Longhi, Catia, additional, Vitanza, Luca, additional, Bolognino, Vanessa, additional, Zagaglia, Carlo, additional, De Castro, Giovanna, additional, Brindisi, Giulia, additional, Schiavi, Laura, additional, De Vittori, Valentina, additional, Reddel, Sofia, additional, Quagliariello, Andrea, additional, Del Chierico, Federica, additional, Putignani, Lorenza, additional, Duse, Marzia, additional, Palamara, Anna Teresa, additional, and Conte, Maria Pia, additional

Published
2020
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22. Challenges in the Management of SARS-CoV2 Infection: The Role of Oral Bacteriotherapy as Complementary Therapeutic Strategy to Avoid the Progression of COVID-19

Author

d'Ettorre, Gabriella, primary, Ceccarelli, Giancarlo, additional, Marazzato, Massimiliano, additional, Campagna, Giuseppe, additional, Pinacchio, Claudia, additional, Alessandri, Francesco, additional, Ruberto, Franco, additional, Rossi, Giacomo, additional, Celani, Luigi, additional, Scagnolari, Carolina, additional, Mastropietro, Cristina, additional, Trinchieri, Vito, additional, Recchia, Gregorio Egidio, additional, Mauro, Vera, additional, Antonelli, Guido, additional, Pugliese, Francesco, additional, and Mastroianni, Claudio Maria, additional

Published
2020
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23. Diet Supplementation, Probiotics, and Nutraceuticals in SARS-CoV-2 Infection: A Scoping Review

Author

Infusino, Fabio, primary, Marazzato, Massimiliano, additional, Mancone, Massimo, additional, Fedele, Francesco, additional, Mastroianni, Claudio Maria, additional, Severino, Paolo, additional, Ceccarelli, Giancarlo, additional, Santinelli, Letizia, additional, Cavarretta, Elena, additional, Marullo, Antonino G. M., additional, Miraldi, Fabio, additional, Carnevale, Roberto, additional, Nocella, Cristina, additional, Biondi-Zoccai, Giuseppe, additional, Pagnini, Cristiano, additional, Schiavon, Sonia, additional, Pugliese, Francesco, additional, Frati, Giacomo, additional, and d’Ettorre, Gabriella, additional

Published
2020
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24. Nasal Microbiota in RSV Bronchiolitis

Author

Schippa, Serena, primary, Frassanito, Antonella, additional, Marazzato, Massimiliano, additional, Nenna, Raffaella, additional, Petrarca, Laura, additional, Neroni, Bruna, additional, Bonfiglio, Giulia, additional, Guerrieri, Francesca, additional, Frasca, Federica, additional, Oliveto, Giuseppe, additional, Pierangeli, Alessandra, additional, and Midulla, Fabio, additional

Published
2020
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25. Bovine Lactoferrin Pre-Treatment Induces Intracellular Killing of AIEC LF82 and Reduces Bacteria-Induced DNA Damage in Differentiated Human Enterocytes

Author

Lepanto, Maria Stefania, primary, Rosa, Luigi, additional, Cutone, Antimo, additional, Scotti, Mellani Jinnett, additional, Conte, Antonietta Lucia, additional, Marazzato, Massimiliano, additional, Zagaglia, Carlo, additional, Longhi, Catia, additional, Berlutti, Francesca, additional, Musci, Giovanni, additional, Valenti, Piera, additional, and Conte, Maria Pia, additional

Published
2019
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29. Characterization of mucosa-associated and fecal microbiota of children with autism spectre disorder

Author

IEBBA, VALERIO, SCHIPPA, Serena, VALITUTTI, FRANCESCO, SANTANGELO, FLORIANA, S. Gatti, I. Celletti, S. Leoni, TROVATO, CHIARA MARIA, IORFIDA, DONATELLA, P. Di Matteo, Marazzato, Massimiliano, CONTE, Maria Pia, BARBATO, Maria, Iebba, Valerio, Schippa, Serena, Valitutti, Francesco, Santangelo, Floriana, S., Gatti, I., Celletti, S., Leoni, Trovato, CHIARA MARIA, Iorfida, Donatella, P., Di Matteo, Marazzato, Massimiliano, Conte, Maria Pia, and Barbato, Maria

Published
2013

31. Lactoferrin differently modulates the inflammatory response in epithelial models mimicking human inflammatory and infectious diseases

Author

Frioni, Alessandra, Conte, Maria Pia, Cutone, Antimo, Longhi, Catia, Giovanni, Musci, BONACCORSI DI PATTI, Maria Carmela, Maria Carmela Bonaccorsi Patti, D. I., Di Patti, M. C. B., Natalizi, Tiziana, Marazzato, Massimiliano, Patrizia, Puddu, Paesano, Rosalba, Valenti, Piera, Berlutti, Francesca, and Lepanto, MARIA STEFANIA

Subjects
Genetics and Molecular Biology (all), Cystic Fibrosis, Chron's disease, Cell, Ferroportin, Biochemistry, Crohn Disease, Models, Pathogen, Cation Transport Proteins, Cells, Cultured, Cultured, biology, Lactoferrin, Medicine (all), Metals and Alloys, medicine.anatomical_structure, Pseudomonas aeruginosa, medicine.symptom, Inflammation Mediators, General Agricultural and Biological Sciences, Infection, Intracellular, Cells, Iron, cystic fibrosis, ferroportin, inflammation, lactoferrin, infection, chron's disease, Inflammation, Bronchi, Respiratory Mucosa, Cystic fibrosis, Animals, Antimicrobial Cationic Peptides, Cattle, Epithelial Cells, Escherichia coli, Humans, Models, Biological, Biomaterials, 2506, Agricultural and Biological Sciences (all), Biochemistry, Genetics and Molecular Biology (all), General Biochemistry, Genetics and Molecular Biology, Microbiology, medicine, Intracellular parasite, Biological, Epithelium, biology.protein
Abstract

Conflicting data are reported on pro- or anti-inflammatory activity of bovine lactoferrin (bLf) in different cell models as phagocytes or epithelial cell lines infected by bacteria. Here we evaluated the bLf effect on epithelial models mimicking two human pathologies characterized by inflammation and infection with specific bacterial species. Primary bronchial epithelium from a cystic fibrosis (CF) patient and differentiated intestinal epithelial cells were infected with Pseudomonas aeruginosa LESB58 isolated from a CF patient and Adherent-Invasive Escherichia coli LF82 isolated from a Crohn's disease patient. Surprisingly, bLf significantly reduced the intracellular bacterial survival, but differently modulated the inflammatory response. These data lead us to hypothesize that bLf differentially acts depending on the epithelial model and infecting pathogen. To verify this hypothesis, we explored whether bLf could modulate ferroportin (Fpn), the only known cellular iron exporter from cells, that, by lowering the intracellular iron level, determines a non permissive environment for intracellular pathogens. Here, for the first time, we describe the bLf ability to up-regulate Fpn protein in infected epithelial models. Our data suggest that the mechanism underlying the bLf modulating activity on inflammatory response in epithelial cells is complex and the bLf involvement in modulating cellular iron homeostasis should be taken into account.

Published
2014

33. The Adherent/Invasive Escherichia coli Strain LF82 Invades and Persists in Human Prostate Cell Line RWPE-1, Activating a Strong Inflammatory Response

Author

Conte, Maria P., primary, Aleandri, Marta, additional, Marazzato, Massimiliano, additional, Conte, Antonietta L., additional, Ambrosi, Cecilia, additional, Nicoletti, Mauro, additional, Zagaglia, Carlo, additional, Gambara, Guido, additional, Palombi, Fioretta, additional, De Cesaris, Paola, additional, Ziparo, Elio, additional, Palamara, Anna T., additional, Riccioli, Anna, additional, and Longhi, Catia, additional

Published
2016
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37. Influenza A Virus Infection of Intestinal Epithelial Cells Enhances the Adhesion Ability of Crohn’s Disease Associated Escherichia coli Strains

Author

Aleandri, Marta, primary, Conte, Maria Pia, additional, Simonetti, Giovanna, additional, Panella, Simona, additional, Celestino, Ignacio, additional, Checconi, Paola, additional, Marazzato, Massimiliano, additional, Longhi, Catia, additional, Goldoni, Paola, additional, Nicoletti, Mauro, additional, Barnich, Nicolas, additional, Palamara, Anna Teresa, additional, Schippa, Serena, additional, and Nencioni, Lucia, additional

Published
2015
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38. Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

Author

Conte, Maria Pia, primary, Longhi, Catia, additional, Marazzato, Massimiliano, additional, Conte, Antonietta Lucia, additional, Aleandri, Marta, additional, Lepanto, Maria Stefania, additional, Zagaglia, Carlo, additional, Nicoletti, Mauro, additional, Aloi, Marina, additional, Totino, Valentina, additional, Palamara, Anna Teresa, additional, and Schippa, Serena, additional

Published
2014
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39. The Shigella flexneriOmpA amino acid residues 188EVQ190are essential for the interaction with the virulence factor PhoN2

Author

Scribano, Daniela, Damico, Rosanna, Ambrosi, Cecilia, Superti, Fabiana, Marazzato, Massimiliano, Conte, Maria Pia, Longhi, Catia, Palamara, Anna Teresa, Zagaglia, Carlo, and Nicoletti, Mauro

Abstract

Shigella flexneriis an intracellular pathogen that deploys an arsenal of virulence factors promoting host cell invasion, intracellular multiplication and intra- and inter-cellular dissemination. We have previously reported that the interaction between apyrase (PhoN2), a periplasmic ATP-diphosphohydrolase, and the C-terminal domain of the outer membrane (OM) protein OmpA is likely required for proper IcsA exposition at the old bacterial pole and thus for full virulence expression of Shigella flexneri(Scribano et al., 2014). OmpA, that is the major OM protein of Gram-negative bacteria, is a multifaceted protein that plays many different roles both in the OM structural integrity and in the virulence of several pathogens. Here, by using yeast two-hybrid technology and by constructing an in silico3D model of OmpA from S. flexneri5a strain M90T, we observed that the OmpA residues 188EVQ190are likely essential for PhoN2-OmpA interaction. The 188EVQ190amino acids are located within a flexible region of the OmpA protein that could represent a scaffold for protein-protein interaction.

Published
2016
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41. The Adherent/Invasive Escherichia coliStrain LF82 Invades and Persists in Human Prostate Cell Line RWPE-1, Activating a Strong Inflammatory Response

Author

Conte, Maria P., Aleandri, Marta, Marazzato, Massimiliano, Conte, Antonietta L., Ambrosi, Cecilia, Nicoletti, Mauro, Zagaglia, Carlo, Gambara, Guido, Palombi, Fioretta, De Cesaris, Paola, Ziparo, Elio, Palamara, Anna T., Riccioli, Anna, and Longhi, Catia

Abstract

ABSTRACTAdherent/invasive Escherichia coli(AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli(ExPEC), neonatal meningitis-associated E. coli(NMEC), and uropathogenic E. coli(UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect prostate cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 prostate cell line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within prostate epithelial cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silicoanalysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitrodata support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.

Published
2016
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43. The effect of probiotic administration on metabolomics and glucose metabolism in CF patients

Author

Michal Gur, Nehama Zuckerman‐Levin, Kamal Masarweh, Moneera Hanna, Luca Laghi, Massimiliano Marazzato, Shir Levanon, Fahed Hakim, Ronen Bar–Yoseph, Michael Wilschanski, Lea Bentur, Gur, Michal, Zuckerman-Levin, Nehama, Masarweh, Kamal, Hanna, Moneera, Laghi, Luca, Marazzato, Massimiliano, Levanon, Shir, Hakim, Fahed, Bar-Yoseph, Ronen, Wilschanski, Michael, and Bentur, Lea

Subjects
Pulmonary and Respiratory Medicine, Adult, Blood Glucose, Indoles, Adolescent, Cystic Fibrosis, glucose metabolism, Glutamine, Pilot Projects, Young Adult, Glucose Intolerance, Diabetes Mellitus, Humans, Pilot Project, Mannitol, Cysteine, Prospective Studies, Child, Cytokine, Cystic Fibrosi, Blood Glucose Self-Monitoring, Probiotics, Diabetes Mellitu, Arabinose, Lactulose, CFRD, Dysbiosi, Prospective Studie, Indole, Pediatrics, Perinatology and Child Health, Lactates, Quality of Life, Lactate, Cytokines, Dysbiosis, Insulin Resistance, probiotic, metabolomic, Human
Abstract

Background and objectives Cystic fibrosis (CF)-related diabetes (CFRD) affects 50% of CF adults. Gut microbial imbalance (dysbiosis) aggravates their inflammatory response and contributes to insulin resistance (IR). We hypothesized that probiotics may improve glucose tolerance by correcting dysbiosis. Methods A single-center prospective pilot study assessing the effect of Vivomixx (R) probiotic (450 billion/sachet) on clinical status, spirometry, lung clearance index (LCI), and quality of life (QOL) questionnaires; inflammatory parameters (urine and stool metabolomics, blood cytokines); and glucose metabolism (oral glucose tolerance test [OGTT]), continuous glucose monitoring [CGM], and homeostasis model assessment of IR (HOMA-IR) in CF patients. Results Twenty-three CF patients (six CFRD), mean age 17.7 +/- 8.2 years. After 4 months of probiotic administration, urinary cysteine (p = 0.018), lactulose (p = 0.028), arabinose (p = 0.036), mannitol (p = 0.041), and indole 3-lactate (p = 0.046) significantly increased, while 3-methylhistidine (p = 0.046) and N-acetyl glutamine (p = 0.047) decreased. Stool 2-Hydroxyisobutyrate (p = 0.022) and 3-methyl-2-oxovalerate (p = 0.034) decreased. Principal component analysis, based on urine metabolites, found significant partitions between subjects at the end of treatment compared to baseline (p = 0.004). After 2 months of probiotics, the digestive symptoms domain of Cystic Fibrosis Questionnaire-Revised improved (p = 0.007). In the nondiabetic patients, a slight decrease in HOMA-IR, from 2.28 to 1.86, was observed. There was no significant change in spirometry results, LCI, blood cytokines and CGM. Conclusions Changes in urine and stool metabolic profiles, following the administration of probiotics, may suggest a positive effect on glucose metabolism in CF. Larger long-term studies are needed to confirm our findings. Understanding the interplay between dysbiosis, inflammation, and glucose metabolism may help preventing CFRD.

Published
2022

44. Oral Bacteriotherapy Reduces the Occurrence of Chronic Fatigue in COVID-19 Patients

Author

Letizia Santinelli, Luca Laghi, Giuseppe Pietro Innocenti, Claudia Pinacchio, Paolo Vassalini, Luigi Celani, Alessandro Lazzaro, Cristian Borrazzo, Massimiliano Marazzato, Lorenzo Tarsitani, Alexia E. Koukopoulos, Claudio M. Mastroianni, Gabriella d'Ettorre, Giancarlo Ceccarelli, Santinelli, Letizia, Laghi, Luca, Innocenti, Giuseppe Pietro, Pinacchio, Claudia, Vassalini, Paolo, Celani, Luigi, Lazzaro, Alessandro, Borrazzo, Cristian, Marazzato, Massimiliano, Tarsitani, Lorenzo, Koukopoulos, Alexia E., Mastroianni, Claudio M., d'Ettorre, Gabriella, and Ceccarelli, Giancarlo

Subjects
Nutrition and Dietetics, Nutrition. Foods and food supply, chronic fatigue, COVID-19, probiotics, metabolomics, FAS, arginine, asparagine, lactate, Endocrinology, Diabetes and Metabolism, Arginine, Lactate, TX341-641, Asparagine, probiotic, Food Science, Nutrition, Original Research, metabolomic
Abstract

Long COVID refers to patients with symptoms as fatigue, “brain fog,” pain, suggesting the chronic involvement of the central nervous system (CNS) in COVID-19. The supplementation with probiotic (OB) would have a positive effect on metabolic homeostasis, negatively impacting the occurrence of symptoms related to the CNS after hospital discharge. On a total of 58 patients hospitalized for COVID-19, 24 (41.4%) received OB during hospitalization (OB+) while 34 (58.6%) taken only the standard treatment (OB–). Serum metabolomic profiling of patients has been performed at both hospital acceptance (T0) and discharge (T1). Six months after discharge, fatigue perceived by participants was assessed by administrating the Fatigue Assessment Scale. 70.7% of participants reported fatigue while 29.3% were negative for such condition. The OB+ group showed a significantly lower proportion of subjects reporting fatigue than the OB– one (p < 0.01). Furthermore, OB+ subjects were characterized by significantly increased concentrations of serum Arginine, Asparagine, Lactate opposite to lower levels of 3-Hydroxyisobutirate than those not treated with probiotics. Our results strongly suggest that in COVID-19, the administration of probiotics during hospitalization may prevent the development of chronic fatigue by impacting key metabolites involved in the utilization of glucose as well as in energy pathways.

Published
2021

45. Escherichia coli Population-Based Study in Pediatric Crohn’s Disease

Author

Serena Schippa, Valentina Totino, Massimiliano Marazzato, Maria Stefania Lepanto, Floriana Santangelo, Marta Aleandri, Antonella Gagliardi, Catia Longhi, Fabrizio Pantanella, Valerio Iebba, Maria Pia Conte, Schippa, Serena, Totino, Valentina, Marazzato, Massimiliano, Stefania Lepanto, Maria, Santangelo, Floriana, Aleandri, Marta, Gagliardi, Antonella, Longhi, Catia, Pantanella, Fabrizio, Iebba, Valerio, and Pia Conte, Maria

Subjects
crohn’s disease, mucosa-associated, escherichia coli
Abstract

Escherichia coli is assumed to be involved in inflammatory bowel disease (IBD) by many authors. The present Short Report was aimed at analyzing E. coli population isolates from ileal samples collected from 4 CD and 3 non-CD (control group) diagnosed pediatric patients. A total of 539 mu- cosa-associated E. coli strains were characterized by: Random Amplified Polymorphic DNA (RAPD), adhesive and virulence factors, and for their phylogenetic groups. A significant separation be- tween RAPD profiles of the two CD and non-CD cohorts (P < 0.0001), along with a significant reduc- tion of intra-species genomic variability in E. coli populations isolated from CD group (P < 0.0001) was found as assessed by Dice index, indicating a different selective pressure in CD intestinal ha- bitat. A predominance of phylogenetic group A was found in control subjects (P < 0.0001). Results on adhesive and virulence factors evidenced peculiar genes significantly related to CD E. coli pop- ulations (K1, IbeA) (P < 0.0001) and to total DNA from biopsy specimens K1 (P < 0.0001). Results suggest that among E. coli population, particular variants may be favorite in the intestinal habitat of CD pediatric patients. These genotype variants could represent the genetic background that, via evolutionary phenomena driven by a persistent inflammatory state, may evolve in Adherent Inva- sive Escherichia coli (AIEC) like strains found in adult CD patients.

Published
2014

46. Characterization of mucosa-associated and fecal microbiota of children with austism spectrum disorder

Author

Maria Barbato, Serena Schippa, Francesco Valitutti, Valerio Iebba, Floriana Santangelo, Chiara Maria Trovato, Stefania Leoni, S. Gatti, Massimiliano Marazzato, Maria Pia Conte, Vincenzo Leuzzi, Salvatore Cucchiara, Barbato, Maria, Schippa, Serena, Valitutti, Francesco, Iebba, Valerio, Santangelo, Floriana, Maria Trovato, Chiara, Leoni, Stefania, Gatti, S., Marazzato, Massimiliano, Pia Conte, Maria, Leuzzi, Vincenzo, and Cucchiara, Salvatore

Published
2012

47. Plasmid-mediated fluoroquinolone resistance determinants in Escherichia coli from community uncomplicated urinary tract infection in an area of high prevalence of quinolone resistance

Author

Lucia Pallecchi, Maria Pia Conte, Massimiliano Marazzato, Valerio Iebba, C. Gallinelli, Antonella Comanducci, Catia Longhi, Valentina Totino, Eleonora Riccobono, Serena Schippa, Floriana Santangelo, Longhi, Catia, Pia Conte, Maria, Marazzato, Massimiliano, Iebba, Valerio, Totino, Valentina, Santangelo, Floriana, Gallinelli, Carmela, Pallecchi, L., Riccobono, E., Schippa, Serena, and Comanducci, Antonella

Subjects
Male, Drug Resistance, Ceftazidime, medicine.disease_cause, Polymerase Chain Reaction, Plasmid, Medical microbiology, Ampicillin, 80 and over, Prevalence, Uropathogenic Escherichia coli, Escherichia coli Infections, Aged, 80 and over, Bacterial, General Medicine, Middle Aged, Anti-Bacterial Agents, Ciprofloxacin, Community-Acquired Infections, Infectious Diseases, Italy, Urinary Tract Infections, Adolescent, Adult, Aged, DNA, Bacterial, Female, Fluoroquinolones, Genes, Bacterial, Humans, Microbial Sensitivity Tests, Plasmids, Young Adult, beta-Lactamases, Drug Resistance, Bacterial, medicine.drug, Microbiology (medical), medicine.medical_specialty, Urinary system, Microbiology, Antibiotic resistance, medicine, Escherichia coli, business.industry, DNA, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Genes, business
Abstract

In Italy fluoroquinolones (FQs) are extensively prescribed in empirical therapy of uncomplicated urinary tract infection (UTI) despite recommendations in national guidelines and widespread antibiotic resistance in community. To survey the dissemination of plasmid-mediated quinolone resistance in a peak area of FQs consumption, E. coli strains from 154 community and 41 local hospital patients were collected; low level ciprofloxacin resistance qnrA, qnrB, qnrS, and aac(6) -Ib-cr genes were screened by PCR and patterns of transferable resistances were determined. Clinical ciprofloxacin resistance in hospital doubled community value, while overall rates of FQ resistance genes were similar (31.6% and 27.8%). Prevalence of aac(6)-Ib-cr gene was 11% in outpatients (21%, inpatients) and risk of harbouring this variant was significantly associated with gentamicin resistance; linkage to ceftazidime resistance was significant (P0 0.001) and six out of eight strains produced CTX-M-15 and TEM-1 beta lactamases. In transconjugants, the unique pattern ampicillin/kanamycin- gentamicin/ ESBL + was associated with aac(6)-Ib-cr gene presence and with an increase of ciprofloxacin MIC value. Data highlight the need to monitor the resistance risk factors in the local community to provide clinicians with well-grounded guidelines for UTI therapy. © Springer-Verlag 2012.

Published
2011

48. Bacterial biofilm associated with a case of capsular contracture.

Author

Conte MP, Superti F, Moio M, Ammendolia MG, Longhi C, Aleandri M, Marazzato M, Goldoni P, Parisi P, Borab Z, Palamara AT, and Carlesimo B

Subjects
Adult, Anti-Bacterial Agents pharmacology, Bacteria drug effects, Bacteria isolation & purification, Bacterial Infections pathology, Female, Humans, Microbial Sensitivity Tests, Bacterial Infections microbiology, Biofilms, Breast Implants adverse effects
Abstract

Capsular contracture is one of the most common complications of implant-based breast augmentation. Despite its prevalence, the etiology of capsular contracture remains controversial although the surface texture of the breast implant, the anatomical position of the prosthesis and the presence of bacterial biofilm could be considered trigger factors. In fact, all medical implants are susceptible to bacterial colonization and biofilm formation. The present study demonstrated the presence of microbial biofilm constituted by cocci in a breast implant obtained from a patient with Baker grade II capsular contracture. This suggests that subclinical infection can be present and involved in low grade capsular contracture.

Published
2018

49. Features of uropathogenic Escherichia coli able to invade a prostate cell line.

Author

Longhi C, Comanducci A, Riccioli A, Ziparo E, Marazzato M, Aleandri M, Conte AL, Lepanto MS, Goldoni P, and Conte MP

Subjects
Adolescent, Adult, Bacterial Adhesion physiology, Cell Line, Female, Humans, Male, Middle Aged, Uropathogenic Escherichia coli classification, Young Adult, Escherichia coli Infections microbiology, Prostate cytology, Urinary Tract Infections microbiology, Uropathogenic Escherichia coli physiology
Abstract

RWPE-1 normal prostate cells were tested as an experimental model for adhesion/invasion assays by genotypically and phenotypically characterized community uropathogenic strains of Escherichia coli (UPEC), a frequent cause of urinary tract infections (UTIs) and significant etiologic agent also in bacterial prostatitis. Adhesive ability and strong biofilm production was significantly associated with the bacterial invasive phenotype. Invasive strains derived mainly from male and pediatric patients. This study suggests that such a cell model could usefully integrate other available methods of urovirulence analysis, to deepen knowledge on the bacterial interaction with host cells.

Published
2016

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