44 results on '"María Julia Ruiz"'
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2. Devenir viejo en la infancia, devenir niño en la vejez. Temporalidades desajustadas en el proyecto autorial de Joaquín Sabina
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María Julia Ruiz
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Infancia ,Vejez ,Devenir ,Proyecto autorial ,Joaquín Sabina ,Language and Literature ,French literature - Italian literature - Spanish literature - Portuguese literature ,PQ1-3999 - Abstract
En el presente trabajo abordaremos el proyecto autorial (Zapata, 2011) de Joaquín Sabina en su etapa de canonización a partir de las figuraciones (Pozuelo Yvancos, 2010) y ficciones teóricas (Link, 2014) de la infancia y la vejez como instrumentos de lectura que emergen en términos de funciones del principio (Premat, 2016) y de funciones del final (Ruiz, 2021a). Indagaremos en autopoéticas textuales y en intervenciones públicas de Sabina, aquellas manifestaciones que exponen las figuraciones de infancia y vejez, para pensarlas no sólo como representaciones sobre el niño que fue o el anciano que es el autor sino también como mecanismos discursivos y comportamentales que elaboran una postura autorial (Meizoz, 2007, 2009) y un personaje de autor (Castilla del Pino, 1989) particular. De esta manera, el arco que traza la vida entre sus extremos habilita la teorización, en términos de devenir (Deleuze y Guattari, 1988), de los espacios de infancia y vejez como laboratorios de escritura (Premat, 2014, 2016), campos creativos donde poner a funcionar la maquinaria de las ficciones, insumos productivos desde donde desajustar las temporalidades para erigir una postura infantil en términos de resistencia.
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- 2022
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3. 'One writes to learn to write.' Interview with Benjamín Prado
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María Julia Ruiz
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benjamín prado ,Latin America. Spanish America ,F1201-3799 ,French literature - Italian literature - Spanish literature - Portuguese literature ,PQ1-3999 - Abstract
Interview with Benjamín Prado.
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- 2021
4. Biological Functions of Exopolysaccharides from Lactic Acid Bacteria and Their Potential Benefits for Humans and Farmed Animals
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María Laura Werning, Annel M. Hernández-Alcántara, María Julia Ruiz, Lorena Paola Soto, María Teresa Dueñas, Paloma López, and Laureano Sebastián Frizzo
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exopolysaccharides ,EPS ,lactic acid bacteria ,LAB ,probiotics ,food-producing animals ,Chemical technology ,TP1-1185 - Abstract
Lactic acid bacteria (LAB) synthesize exopolysaccharides (EPS), which are structurally diverse biopolymers with a broad range of technological properties and bioactivities. There is scientific evidence that these polymers have health-promoting properties. Most commercialized probiotic microorganisms for consumption by humans and farmed animals are LAB and some of them are EPS-producers indicating that some of their beneficial properties could be due to these polymers. Probiotic LAB are currently used to improve human health and for the prevention and treatment of specific pathologic conditions. They are also used in food-producing animal husbandry, mainly due to their abilities to promote growth and inhibit pathogens via different mechanisms, among which the production of EPS could be involved. Thus, the aim of this review is to discuss the current knowledge of the characteristics, usage and biological role of EPS from LAB, as well as their postbiotic action in humans and animals, and to predict the future contribution that they could have on the diet of food animals to improve productivity, animal health status and impact on public health.
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- 2022
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5. Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
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María Julia Ruiz, Jimena Salido, Lorena Abusamra, Yanina Ghiglione, Cintia Cevallos, Gabriel Damilano, Ana María Rodriguez, César Trifone, Natalia Laufer, Luis D. Giavedoni, Omar Sued, Horacio Salomón, María Magdalena Gherardi, and Gabriela Turk
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HIV-1 ,Serodiscordant couples ,ADCC ,HIV-1 transmission ,Medicine ,Medicine (General) ,R5-920 - Abstract
As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV− donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection.
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- 2017
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6. Imagen, postura, proyecto. Apuestas a un nuevo abordaje de la figura del autor
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María Julia Ruiz
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autor ,imagen ,postura ,proyecto autorial ,puesta en escena ,Philology. Linguistics ,P1-1091 ,Discourse analysis ,P302-302.87 - Abstract
La vuelta del autor, tanto a las páginas literarias como a las agendas de investigación, manifiesta una de las problemáticas centrales sobre el sujeto en el siglo XXI. La misma pertenece a todos los ámbitos teóricos de las ciencias humanas y exige una revisión, una problematización, un abordaje múltiple. Este sujeto que regresa -figura ponderada y olvidada en el tiempo, muerta y resurrecta- adopta nuevas formas de expresarse y de posicionarse no sólo en sus textos sino en el mundo real. La pregunta inicial versa sobre cómo abordar este concepto errático de autor que se compone en un cincuenta por ciento de lenguaje y en un cincuenta por ciento de realidad, mitad hombre y mitad simulacro, mitad sujeto y mitad discurso. El marco teórico que ensamblamos busca dar respuesta a esta pregunta mediante las metáforas escénicas, aquellas que permiten indagar en los procedimientos de puesta en escena del autor como parte de la construcción de un proyecto autorial. Este artículo, a través de una metodología cualitativa, analiza las categorías de proyecto autorial (Zapata), imagen de autor (Maingueneau, Amossy y Gramuglio) y postura de autor (Meizoz).
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- 2019
7. Phenotype, Polyfunctionality, and Antiviral Activity of in vitro Stimulated CD8+ T-Cells From HIV+ Subjects Who Initiated cART at Different Time-Points After Acute Infection
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Jimena Salido, María Julia Ruiz, César Trifone, María Inés Figueroa, María Paula Caruso, María Magdalena Gherardi, Omar Sued, Horacio Salomón, Natalia Laufer, Yanina Ghiglione, and Gabriela Turk
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HIV functional cure ,expanded CD8+ T-cell response ,polyfunctionality and phenotype ,antiviral activity ,time of cART initiation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Since anti-HIV treatment cannot cure the infection, many strategies have been proposed to eradicate the viral reservoir, which still remains as a major challenge. The success of some of these strategies will rely on the ability of HIV-specific CD8+ T-cells (CD8TC) to clear reactivated infected cells. Here, we aimed to investigate the phenotype and function of in vitro expanded CD8TC obtained from HIV+ subjects on combination antiretroviral therapy (cART), either initiated earlier (median = 3 months postinfection, ET: Early treatment) or later (median = 20 months postinfection, DT: Delayed treatment) after infection. Peripheral blood mononuclear cells from 12 DT and 13 ET subjects were obtained and stimulated with Nef and Gag peptide pools plus IL-2 for 14 days. ELISPOT was performed pre- and post-expansion. CD8TC memory/effector phenotype, PD-1 expression, polyfunctionality (CD107a/b, IFN-γ, IL-2, CCL4 (MIP-1β), and/or TNF-α production) and antiviral activity were evaluated post-expansion. Magnitude of ELISPOT responses increased after expansion by 103 times, in both groups. Expanded cells were highly polyfunctional, regardless of time of cART initiation. The memory/effector phenotype distribution was sharply skewed toward an effector phenotype after expansion in both groups although ET subjects showed significantly higher proportions of stem-cell and central memory CD8TCs. PD-1 expression was clustered in HIV-specific effector memory CD8TCs, subset that also showed the highest proportion of cytokine–producing cells. Moreover, PD-1 expression directly correlated with CD8TC functionality. Expanded CD8TCs from DT and ET subjects were highly capable of mediating antiviral activity, measured by two different assays. Antiviral function directly correlated with the proportion of fully differentiated effector cells (viral inhibition assay) as well as with CD8TC polyfunctionality and PD-1 expression (VITAL assay). In sum, we show that, despite being dampened in subjects on cART, the HIV-specific CD8TC response could be selectively stimulated and expanded in vitro, presenting a high proportion of cells able to carry-out multiple effector functions. Timing of cART initiation had an impact on the memory/effector differentiation phenotype, most likely reflecting how different periods of antigen persistence affected immune function. Overall, these results have important implications for the design and evaluation of strategies aimed at modulating CD8TCs to achieve the HIV functional cure.
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- 2018
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8. Interaction Between Macrophage Migration Inhibitory Factor and CD74 in Human Immunodeficiency Virus Type I Infected Primary Monocyte-Derived Macrophages Triggers the Production of Proinflammatory Mediators and Enhances Infection of Unactivated CD4+ T Cells
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César Trifone, Jimena Salido, María Julia Ruiz, Lin Leng, María Florencia Quiroga, Horacio Salomón, Richard Bucala, Yanina Ghiglione, and Gabriela Turk
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human immunodeficiency virus ,CD74 ,macrophage migration inhibitory factor ,primary monocyte-derived macrophages ,CD4+ T-cells ,immunopathogenesis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Understanding the mechanisms of human immunodeficiency virus type I (HIV-1) pathogenesis would facilitate the identification of new therapeutic targets to control the infection in face of current antiretroviral therapy limitations. CD74 membrane expression is upregulated in HIV-1-infected cells and the magnitude of its modulation correlates with immune hyperactivation in HIV-infected individuals. In addition, plasma level of the CD74 activating ligand macrophage migration inhibitory factor (MIF) is increased in infected subjects. However, the role played by MIF/CD74 interaction in HIV pathogenesis remains unexplored. Here, we studied the effect of MIF/CD74 interaction on primary HIV-infected monocyte-derived macrophages (MDMs) and its implications for HIV immunopathogenesis. Confocal immunofluorescence analysis of CD74 and CD44 (the MIF signal transduction co-receptor) expression indicated that both molecules colocalized at the plasma membrane specifically in wild-type HIV-infected MDMs. Treatment of infected MDMs with MIF resulted in an MIF-dependent increase in TLR4 expression. Similarly, there was a dose-dependent increase in the production of IL-6, IL-8, TNFα, IL-1β, and sICAM compared to the no-MIF condition, specifically from infected MDMs. Importantly, the effect observed on IL-6, IL-8, TNFα, and IL-1β was abrogated by impeding MIF interaction with CD74. Moreover, the use of a neutralizing αMIF antibody or an MIF antagonist reverted these effects, supporting the specificity of the results. Treatment of unactivated CD4+ T-cells with MIF-treated HIV-infected MDM-derived culture supernatants led to enhanced permissiveness to HIV-1 infection. This effect was lost when CD4+ T-cells were treated with supernatants derived from infected MDMs in which CD74/MIF interaction had been blocked. Moreover, the enhanced permissiveness of unactivated CD4+ T-cells was recapitulated by exogenous addition of IL-6, IL-8, IL-1β, and TNFα, or abrogated by neutralizing its biological activity using specific antibodies. Results obtained with BAL and NL4-3 HIV laboratory strains were reproduced using transmitted/founder primary isolates. This evidence indicated that MIF/CD74 interaction resulted in a higher production of proinflammatory cytokines from HIV-infected MDMs. This caused the generation of an inflammatory microenvironment which predisposed unactivated CD4+ T-cells to HIV-1 infection, which might contribute to viral spreading and reservoir seeding. Overall, these results support a novel role of the MIF/CD74 axis in HIV pathogenesis that deserves further investigation.
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- 2018
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9. From farm to table: follow-up of Shiga toxin-producing Escherichia coli throughout the pork production chain in Argentina
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Rocío eColello, María Emilia Cáceres, María Julia Ruiz, Marcelo eSanz, Analía Inés Etcheverría, and Nora Lía ePadola
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STEC ,Prevalence ,characterization ,foodborne pathogens ,pork production chain ,Microbiology ,QR1-502 - Abstract
Pigs are important reservoirs of Shiga toxin-producing Escherichia coli (STEC). The entrance of these strains into the food chain implies a risk to consumers because of the severity of hemolytic uremic syndrome. This study reports the prevalence and characterization of Shiga toxin-producing Escherichia coli (STEC) throughout the pork production chain. From 764 samples, 31 (4.05%) were stx positive by PCR screening. At farms, 2.86% of samples were stx positive; at slaughter, 4.08% of carcasses were stx positive and at boning rooms, 6% of samples were stx positive. These percentages decreased in pork meat ready for sale at sales markets (4.59%). From positive samples, 50 isolates could be characterized. At farms 37.5% of the isolates carried stx1/stx2 genes, 37.5% possessed stx2e and 25%, carried only stx2. At slaughter we detected 50% of isolates positive for stx2, 33% for stx2e and 16% for stx1/stx2. At boning rooms 59% of the isolates carried stx1/stx2, 14% stx2e and 5% stx1/stx2/stx2e. At retail markets 66% of isolates were positive for stx2, 17% stx2e and 17% stx1/stx2. For the other virulence factors, ehxA and saa were not detected and eae gene was detected in 12% of the isolates. Concerning putative adhesins, agn43 was detected in 72%, ehaA in 26%, aida in 8% and iha in 6% of isolates. The strains were typed into 14 E. coli O groups (O1, O2, O8, O15, O20, O35, O69, O78, O91, O121, O138, O142, O157, O180) and ten H groups (H9, H10, H16, H21, H26, H29, H30, H32, H45, H46). This study reports the prevalence and characterization of STEC strains through the chain pork suggesting the vertical transmission. STEC contamination originates in the farms and is transferred from pigs to carcasses in the slaughter process and increase in meat pork at boning rooms and sales markets. These results highlight the need to implement an integrated STEC control system based on good management practices on the farm and critical control point systems in the food chain.
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- 2016
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10. Biomarkers of Progression after HIV Acute/Early Infection: Nothing Compares to CD4+ T-cell Count?
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Gabriela Turk, Yanina Ghiglione, Macarena Hormanstorfer, Natalia Laufer, Romina Coloccini, Jimena Salido, César Trifone, María Julia Ruiz, Juliana Falivene, María Pía Holgado, María Paula Caruso, María Inés Figueroa, Horacio Salomón, Luis D. Giavedoni, María de los Ángeles Pando, María Magdalena Gherardi, Roberto Daniel Rabinovich, Pedro A. Pury, and Omar Sued
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HIV ,biomarkers ,acute infection ,disease progression ,decision trees ,soluble plasma factors ,HLA ,immune responses ,Microbiology ,QR1-502 - Abstract
Progression of HIV infection is variable among individuals, and definition disease progression biomarkers is still needed. Here, we aimed to categorize the predictive potential of several variables using feature selection methods and decision trees. A total of seventy-five treatment-naïve subjects were enrolled during acute/early HIV infection. CD4+ T-cell counts (CD4TC) and viral load (VL) levels were determined at enrollment and for one year. Immune activation, HIV-specific immune response, Human Leukocyte Antigen (HLA) and C-C chemokine receptor type 5 (CCR5) genotypes, and plasma levels of 39 cytokines were determined. Data were analyzed by machine learning and non-parametric methods. Variable hierarchization was performed by Weka correlation-based feature selection and J48 decision tree. Plasma interleukin (IL)-10, interferon gamma-induced protein (IP)-10, soluble IL-2 receptor alpha (sIL-2Rα) and tumor necrosis factor alpha (TNF-α) levels correlated directly with baseline VL, whereas IL-2, TNF-α, fibroblast growth factor (FGF)-2 and macrophage inflammatory protein (MIP)-1β correlated directly with CD4+ T-cell activation (p < 0.05). However, none of these cytokines had good predictive values to distinguish “progressors” from “non-progressors”. Similarly, immune activation, HIV-specific immune responses and HLA/CCR5 genotypes had low discrimination power. Baseline CD4TC was the most potent discerning variable with a cut-off of 438 cells/μL (accuracy = 0.93, κ-Cohen = 0.85). Limited discerning power of the other factors might be related to frequency, variability and/or sampling time. Future studies based on decision trees to identify biomarkers of post-treatment control are warrantied.
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- 2018
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11. Early skewed distribution of total and HIV-specific CD8+ T-cell memory phenotypes during primary HIV infection is related to reduced antiviral activity and faster disease progression.
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Yanina Ghiglione, Juliana Falivene, María Julia Ruiz, Natalia Laufer, María Eugenia Socías, Pedro Cahn, Luis Giavedoni, Omar Sued, María Magdalena Gherardi, Horacio Salomón, and Gabriela Turk
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Medicine ,Science - Abstract
The important role of the CD8+ T-cells on HIV control is well established. However, correlates of immune protection remain elusive. Although the importance of CD8+ T-cell specificity and functionality in virus control has been underscored, further unraveling the link between CD8+ T-cell differentiation and viral control is needed. Here, an immunophenotypic analysis (in terms of memory markers and Programmed cell death 1 (PD-1) expression) of the CD8+ T-cell subset found in primary HIV infection (PHI) was performed. The aim was to seek for associations with functional properties of the CD8+ T-cell subsets, viral control and subsequent disease progression. Also, results were compared with samples from Chronics and Elite Controllers. It was found that normal maturation of total and HIV-specific CD8+ T-cells into memory subsets is skewed in PHI, but not at the dramatic level observed in Chronics. Within the HIV-specific compartment, this alteration was evidenced by an accumulation of effector memory CD8+ T (TEM) cells over fully differentiated terminal effector CD8+ T (TTE) cells. Furthermore, higher proportions of total and HIV-specific CD8+ TEM cells and higher HIV-specific TEM/(TEM+TTE) ratio correlated with markers of faster progression. Analysis of PD-1 expression on total and HIV-specific CD8+ T-cells from PHI subjects revealed not only an association with disease progression but also with skewed memory CD8+ T-cell differentiation. Most notably, significant direct correlations were obtained between the functional capacity of CD8+ T-cells to inhibit viral replication in vitro with higher proportions of fully-differentiated HIV-specific CD8+ TTE cells, both at baseline and at 12 months post-infection. Thus, a relationship between preservation of CD8+ T-cell differentiation pathway and cell functionality was established. This report presents evidence concerning the link among CD8+ T-cell function, phenotype and virus control, hence supporting the instauration of early interventions to prevent irreversible immune damage.
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- 2014
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12. Calidad microbiológica de la carne picada y detección de patógenos en muestras ambientales de carnicerías de la ciudad de Tandil, provincia de Buenos Aires, Argentina
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Juan Antonio Passucci, María Julia Ruiz, Edgardo Rodríguez, Rocío Colello, Alejandra Krüger, Marcelo E. Sanz, Gerardo Anibal Leotta, Daniel Fernández Fellenz, Analía Inés Etcheverría, Elida Elichiribehety, and Nora Lía Padola
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Microbiology (medical) ,Agricultural science ,Geography ,Hygiene ,media_common.quotation_subject ,General Medicine ,Microbiological quality ,Microbiology ,Butcher ,media_common - Abstract
The aim of this work was to evaluate the hygienic-sanitary conditions of butcher shops in Tandil, Buenos Aires Province, by estimating the risk based on good manufacturing and hygiene practices, through surveys of the establishments. The analysis was performed using a scale of 1-100, and classifying them as high risk (0-40), moderate risk (41-70) or low risk (71-100). The presence of Salmonella spp., Staphylococcus aureus and Shiga toxin-producing Escherichia coli (STEC) from both, ground beef and environmental samples such as countertop, cleaver, mincer and butcher's hands, taken at butcher shops was also evaluated. Sampling was performed only once and immediately refrigerated and transported to the laboratory for analysis. All butcher shops evaluated (100) were classified as "low risk" with good hygienic-sanitary conditions. However, 75% of the ground beef samples analyzed did not meet at least one of the microbiological criteria established in the Codigo Alimentario Argentino [Argentine Food Code], article 255. We propose to establish a strategy to identify deviations and implement a plan for continuous improvement in butcher shops of Tandil city.
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- 2022
13. Empezar haciendo 'Inventario'. Fábulas del comienzo y la vejez en el proyecto autorial de Joaquín Sabina
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María Julia Ruiz
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General Medicine - Abstract
La infancia y la vejez, además de ser etapas biológicas en la vida de los sujetos, pueden operar como insumos teóricos potentes a la hora de indagar los vínculos entre la vida y el arte. En la presente oportunidad realizaremos un abordaje de aquellas “funciones del principio”, fábulas del comienzo y de la infancia de la escritura (Premat 2016) en la canción “Inventario” de Joaquín Sabina incluida en el álbum homónimo (1978) y recopilada en el volumen autopoético Con buena letra. Edición actualizada (2002, 2007). En esta canción obertura Joaquín Sabina inicia su proyecto autorial (Zapata 2011) en la etapa de emergencia, exponiendo una serie de imágenes (Amossy, Maingueneau 2009) que se convertirán en posturas (Meizoz 2007, 2009) y que delinearán una figura autorial particular, un personaje de autor (Castilla del Pino 1989) que se diseña a sí mismo (Groys 2014) en la búsqueda de una fabricación identitaria original que lo posicione de una determinada manera en la escena poético-musical de la canción de autor. A través de las notas autopoéticas dibujadas en caligrafía manuscrita en Con buena letra, indagaremos cómo Joaquín Sabina revisa su proyecto autorial a la vez que lo funda desde los íncipits de su escritura, entrada en obra y entrada en la literatura, la cual ya desde sus inicios muestra reiterados y paradójicos vestigios de vejez.
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- 2022
14. Envejecer en la infancia. Masculinidad, vejez e identidad en Lo niego todo de Joaquín Sabina
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María Julia Ruiz
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En el presente artículo indagaremos cómo en las letras de las canciones del disco Lo niego todo de Joaquín Sabina y en las declaraciones promocionales en los medios, el cantautor reconfigura su imagen de autor y problematiza su postura, aquella que lo ha acompañado durante todo su proyecto autorial para elaborar un posicionamiento nuevo: el de una masculinidad en declive que, en su proceso de envejecimiento, vuelve hacia el terreno de la infancia para ejercer resistencia desde una postura infantil. Esta masculinidad en declive se manifiesta desde la imagen de la otredad, pues el sujeto que envejece necesita reacomodar su identidad y reconstituirla. El cuerpo del varón viejo, en su pérdida de potencia sexual, de poder, de jerarquía, expone los mandatos de una masculinidad imposible. El disco de estudio Lo niego todo y el libro autopoético que lo acompaña Incluso la verdad manifiestan los mecanismos y operatorias de exclusión para con la vejez en el género masculino, a la vez que ofrecen distintas maneras de zanjar y burlar esos mandatos preestablecidos en nuestra cultura para aprender a “envejecer sin dignidad”.
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- 2022
15. Becoming old in childhood, becoming a child in old age. Misaligned temporalities in the authorial project of Joaquín Sabina
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María Julia Ruiz
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proyecto autorial ,becoming ,devenir ,authorial project ,Literatura ,General Medicine ,Infancia ,Joaquín Sabina ,Vejez ,Letras ,childhood ,old age - Abstract
En el presente trabajo abordaremos el proyecto autorial (Zapata, 2011) de Joaquín Sabina en su etapa de canonización a partir de las figuraciones (Pozuelo Yvancos, 2010) y ficciones teóricas (Link, 2014) de la infancia y la vejez como instrumentos de lectura que emergen en términos de funciones del principio (Premat, 2016) y de funciones del final (Ruiz, 2021a). Indagaremos en autopoéticas textuales y en intervenciones públicas de Sabina, aquellas manifestaciones que exponen las figuraciones de infancia y vejez, para pensarlas no sólo como representaciones sobre el niño que fue o el anciano que es el autor sino también como mecanismos discursivos y comportamentales que elaboran una postura autorial (Meizoz, 2007, 2009) y un personaje de autor (Castilla del Pino, 1989) particular. De esta manera, el arco que traza la vida entre sus extremos habilita la teorización, en términos de devenir (Deleuze y Guattari, 1988), de los espacios de infancia y vejez como laboratorios de escritura (Premat, 2014, 2016), campos creativos donde poner a funcionar la maquinaria de las ficciones, insumos productivos desde donde desajustar las temporalidades para erigir una postura infantil en términos de resistencia., In the present work we will approach the authorial project (Zapata, 2011) of Joaquín Sabina in it stage of canonization from the figurations (Pozuelo Yvancos, 2010) and theoretical fictions (Link, 2014) of childhood and old age as reading instruments that they emerge in terms of functions of the beginning (Premat, 2016) and functions of the end (Ruiz, 2021a). We will investigate in textual autopoetics and in Sabina's public interventions those manifestations that expose the figurations of childhood and old age, to think of them not only as representations about the child who was or the old man who is the author, but also as discursive and behavioral mechanisms that elaborate a posture authorial (Meizoz, 2007, 2009) and a particular author character (Castilla del Pino, 1989). In this way, the arc that traces life between its extremes enables the theorization, in terms of becoming (Deleuze and Guattari, 1988), of the spaces of childhood and old age as writing laboratories (Premat, 2014, 2016), creative fields where to put to work the machinery of fictions, productive inputs from which to misalign temporalities to erect a childish posture in terms of resistance., Dossier: Infancia y vejez en la literatura española: cruces, modulaciones, figuras, Facultad de Humanidades y Ciencias de la Educación
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- 2022
16. Capacidad de Lactiplantibacillus plantarum LP5 para inhibir biopelículas de Campylobacter coli
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Laureano Sebastián Frizzo, Marcelo Lisandro Signorini, Marcelo Raúl Rosmini, Carolina Raquel Olivero, Gabriel Jorge Sequeira, María Victoria Zbrun, Lorena Paola Soto, Jorge Alberto Zimmermann, Noelí Estefanía Sirini, and María Julia Ruiz
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Abiotic Factors ,biopelículas ,superficie abiótica ,Lactiplantibacillus plantarum ,Campylobacter coli ,biofilms ,Factores Abióticos ,abiotic surface ,Lactobacillus plantarum - Abstract
El objetivo de este estudio fue evaluar la capacidad inhibitoria de Lactiplantibacillus plantarum LP5 frente a Campylobacter coli en ensayos de formación de biopelículas in vitro y exclusión competitiva. La formación de biopelículas por C. coli NCTC11366, C. coli DSPV458, C. coli DSPV541 y C. coli DSPV570 fue evaluada mediante medición de DO. La capacidad inhibitoria de L. plantarum LP5 frente a C. coli fue evaluada sobre discos de vidrio, nailon y aluminio. Sobre una biopelícula de L. plantarum se adicionó C. coli para cuantificar el efecto inhibidor de L. plantarum LP5 sobre el patógeno. Las cuatro cepas de C. coli fueron clasificadas como moderadas formadoras de biopelículas. El ensayo de exclusión competitiva mostró que la formación de biopelículas de las cepas de C. coli en todos los materiales fue significativamente mayor que la formación de biopelículas de cada patógeno en presencia de biopelículas de L. plantarum LP5. Si bien es necesario realizar más pruebas para confirmar la capacidad de supervivencia de C. coli en ambientes hostiles hasta llegar al huésped, este estudio permitiría avanzar en el esclarecimiento de su comportamiento mediante la formación de biopelículas. The objective of this study was to evaluate the inhibitory capacity of Lactiplantibacillus plantarum LP5 against Campylobacter coli in in vitro biofilm formation and competitive exclusion assays. Biofilm formation by C. coli NCTC11366, C. coli DSPV458, C. coli DSPV541 and C. coli DSPV570 was evaluated by OD measurement. The inhibitory capacity of L. plantarum LP5 against C. coli was evaluated on glass, nylon and aluminium discs, added with L. plantarum and incubated at 37°C for 72 h. C. coli was added to each washed well. The plates were incubated at 42°C for 72 h in microaerophilic conditions and the biofilms were detached for quantification. The four strains of C. coli were classified as moderate biofilm former. The competitive exclusion test showed that the biofilm formation of the C. coli strains in all materials was significantly higher than the biofilm formation of each pathogen in the presence of L. plantarum LP5 biofilms. Although it is necessary to carry out more tests to confirm the ability of C. coli to survive in hostile environments until reaching the host, this study would allow progress in the elucidation of its behaviour through the formation of biofilms. EEA Rafaela Fil: Ruiz, M.J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ruiz, M.J. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ruiz, M.J. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina Fil: Sirini, Noelí Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Sirini, Noelí Estefanía. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Zimmermann, Jorge Alberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Zbrun, María Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina. Fil: Olivero, Carolina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Olivero, Carolina Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Rosmini, Marcelo Raúl. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina. Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Signorini, Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
- Published
- 2022
17. What are we going to do with od age? Functions of the end and author's postures in 'Lo niego todo' by Joaquín Sabina
- Author
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María Julia Ruiz
- Subjects
Literature and Literary Theory ,Old age ,Humanidades ,Functions of the end ,Joaquín Sabina ,Language and Linguistics ,Authorial position ,Postura autorial ,Humanities ,Literature ,Lo niego todo ,Funciones del final ,Literatura ,Vejez - Abstract
En este trabajo abordaremos un corpus de letras de canciones de la última producción discográfica del proyecto autorial de Joaquín Sabina, Lo niego todo, perteneciente a su etapa de canonización, a partir de las nociones de figuración y ficción teórica de la vejez como instrumentos de lectura que emergen en términos de funciones del final. Indagaremos en aquellas manifestaciones que exponen las operaciones de vejez para pensarlas como mecanismos discursivos y comportamentales que elaboran una postura autorial y un personaje de autor particular. De esta manera, el arco que traza la vida entre sus extremos habilita la teorización de los espacios de infancia y vejez como laboratorios de escritura, como campos creativos donde poner a funcionar la maquinaria de las ficciones, como insumos productivos desde donde desajustar las temporalidades para erigir una postura infantil en términos de resistencia., In this work we will address a corpus of song lyrics from the latest record production of Joaquín Sabina’s authorial project, Lo niego todo, belonging to his canonization stage, based on the notions of figuration and theoretical fiction of old age as reading instruments. that emerge in terms of functions of the end. We will investigate those manifestations that expose the operations of old age to think of them as discursive and behavioral mechanisms that elaborate an authorial posture and a particular author character. In this way, the arc that traces life between its extremes enables the theorization of the spaces of childhood and old age as writing laboratories, as creative fields where to put the machinery of fictions to work, as productive inputs from which to misalign temporalities to erect a childish posture in terms of resistance.
- Published
- 2022
18. Verónica LEUCI, 'Poetas in-versos. Ficción y nombre propio en Gloria Fuertes y Ángel González'. Villa María, Eudevim, 2018, 204 pp
- Author
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María Julia Ruiz
- Subjects
General Medicine - Abstract
Reseña de Verónica Leuci, Poetas in-versos. Ficción y nombre propio en Gloria Fuertes y Ángel González. Villa María, Eudevim, 2018, 204 pp.
- Published
- 2021
19. Massive sequencing of artisan cheeses from raw sheep’s milk
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María Julia Ruiz, L. M. Medina, Analía Inés Etcheverría, Alejandra Krüger, and Nora Lía Padola
- Subjects
Geography ,bacteria ,food and beverages ,Humanities - Abstract
Lactic acid bacteria (LAB) are used in the food industry to confer aromatic characteristics and their antibacterial capacity. In this study the native flora of LAB that participates in the traditional fermentation of semi-hard cheeses made with raw sheep's milk from the region of Andalusia, Spain was analyzed. Three samples of four different commercial cheeses were taken. Massive sequencing was carried out to identify the lactic and accompanying flora. Predominant lactic flora was Lactococcus lactis, Streptococcus thermophilus, Lactobacillus paracasei and Lactococcus raffinolactis, and to a lesser extent other species of the genera Lactobacillus, Streptococcus, Pediococcus and Leuconestoc. The accompanying flora was composed of species of the genera Mycoplasma, Pseudomonas, Acinetobacter, Chryseobacterium, Mannheimia, Trueperella, Enterococcus, Vibrio, Serratia, Macrococcus, Staphylococcus, Massilia, Flavobacterium, Yersinia, Gallaecimonas, Hafnia, Leclercia, Obesumbacterium, Morganella and Kluyvera. These results show that modern molecular techniques are very good tools to identify natural LABs of artisanal dairy products. The characterization of the native flora of the artisanal cheese allows us to evaluate the microbiological diversity of the natural population of LAB and the symbiosis with another type of flora.
- Published
- 2019
20. PD-1 Expression in HIV-Specific CD8+ T cells Before Antiretroviral Therapy Is Associated With HIV Persistence
- Author
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Horacio Salomón, María Laura Polo, Omar Sued, César Ariel Trifone, María Julia Ruiz, Sharon R Lewin, Yanina Alexandra Ghiglione, Natalia Laufer, Ajantha Rhodes, Gabriela Turk, and Jimena Salido
- Subjects
Programmed Cell Death 1 Receptor ,HIV Infections ,CD8-Positive T-Lymphocytes ,030312 virology ,Lymphocyte Activation ,law.invention ,Flow cytometry ,Cohort Studies ,03 medical and health sciences ,Immune system ,law ,Secondary Prevention ,medicine ,Humans ,Cytotoxic T cell ,Pharmacology (medical) ,Polymerase chain reaction ,0303 health sciences ,medicine.diagnostic_test ,business.industry ,Effector ,virus diseases ,Viral Load ,Phenotype ,Treatment Outcome ,Infectious Diseases ,Immunology ,business ,Immunologic Memory ,Viral load ,CD8 - Abstract
BACKGROUND: The persistence of latently infected T cells remains the principal barrier to HIV cure. Understanding how the early immune responses shape persistence of HIV on antiretroviral therapy (ART) will be fundamental for potential eradication. Here, we aimed to determine the relationship between CD8 T-cell function and phenotype before therapy and HIV persistence on ART. METHODS: Blood samples from 29 individuals enrolled during primary HIV infection (at baseline and every 3 months up to 2 years post-ART initiation) were obtained. HIV-specific T-cell function and expression of the activation markers were evaluated before ART by flow cytometry. Cell-associated HIV DNA and unspliced (US)-RNA were quantified in purified CD4 T cells by real-time polymerase chain reaction. Data were analyzed using nonparametric statistics. RESULTS: Elevated immune activation, dominance of monofunctional CD8 T cells, and skewed distribution of memory profile were observed before ART. After ART initiation, HIV DNA and US-RNA levels rapidly diminished, reaching a plateau by 30 weeks after ART. The proportion of baseline HIV-specific effector memory and terminal effector CD8 T cells directly correlated with HIV DNA levels at 1 year after ART. A strong positive correlation was observed between the proportion of bulk and HIV-specific PD-1 CD8 T cells measured before ART and HIV DNA at 1 year after ART. CONCLUSIONS: A higher proportion of terminally differentiated CD8 T cells and increased PD1 expression were associated with HIV persistence on ART after treatment of primary infection. Thus, the quality of the early CD8 T-cell immune response may serve as a predictor of HIV persistence on ART.
- Published
- 2019
21. [Microbiological quality of fresh ground beef and detection of pathogens in environmental samples taken from butcher shops in the city of Tandil, Buenos Aires Province, Argentina]
- Author
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María Julia, Ruiz, Nora Lia, Padola, Gerardo, Leotta, Rocío, Colello, Juan, Passucci, Edgardo, Rodríguez, Daniel, Fernández Fellenz, Alejandra, Krüger, Marcelo, Sanz, Elida, Elichiribehety, and Analía Inés, Etcheverría
- Subjects
Staphylococcus aureus ,Meat ,Shiga-Toxigenic Escherichia coli ,Salmonella ,Argentina ,Food Microbiology ,Animals ,Cattle - Abstract
The aim of this work was to evaluate the hygienic-sanitary conditions of butcher shops in Tandil, Buenos Aires Province, by estimating the risk based on good manufacturing and hygiene practices, through surveys of the establishments. The analysis was performed using a scale of 1-100, and classifying them as high risk (0-40), moderate risk (41-70) or low risk (71-100). The presence of Salmonella spp., Staphylococcus aureus and Shiga toxin-producing Escherichia coli (STEC) from both, ground beef and environmental samples such as countertop, cleaver, mincer and butcher's hands, taken at butcher shops was also evaluated. Sampling was performed only once and immediately refrigerated and transported to the laboratory for analysis. All butcher shops evaluated (100) were classified as "low risk" with good hygienic-sanitary conditions. However, 75% of the ground beef samples analyzed did not meet at least one of the microbiological criteria established in the Código Alimentario Argentino [Argentine Food Code], article 255. We propose to establish a strategy to identify deviations and implement a plan for continuous improvement in butcher shops of Tandil city.
- Published
- 2020
22. Distinguishing Between Inter-domain and Intra-domain Emergence
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María Julia Ruiz and Olimpia Lombardi
- Subjects
Philosophy of science ,Multidisciplinary ,History and Philosophy of Science ,Inter-domain ,060302 philosophy ,05 social sciences ,Ontic ,06 humanities and the arts ,Sociology ,0509 other social sciences ,050905 science studies ,0603 philosophy, ethics and religion ,Epistemology - Abstract
Currently, there are almost as many conceptions of emergence as authors who address the issue. Most literature on the matter focuses either on discussing, evaluating and comparing particular contributions or accounts of emergence, or on assessing a particular case study. Our aim in this paper is rather different. We here set out to introduce a distinction that has not been sufficiently taken into account in previous discussions on this topic: the distinction between inter-domain emergence—a relation between items belonging to different ontic domains—and intra-domain emergence—a relation between items belonging to a same ontic domain. Our final purpose is not to assume and defend a definite stance on emergence, but to stress the relevance of such distinction when attempting to argue for or against emergence, in the first place. We will also address the connections between emergence so distinguished and more general philosophical perspectives, suggesting where would reductionists and pluralists stand with respect to intra- and inter-domain emergence.
- Published
- 2018
23. 'Dealing with the changeable and blurry edges of living things: a modified version of property-cluster kinds'
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Jon Umerez and María Julia Ruiz
- Subjects
Philosophy of science ,Natural kind ,Property (philosophy) ,Computer science ,media_common.quotation_subject ,05 social sciences ,06 humanities and the arts ,050905 science studies ,0603 philosophy, ethics and religion ,Epistemology ,Living systems ,Faith ,Philosophy ,History and Philosophy of Science ,060302 philosophy ,Natural (music) ,0509 other social sciences ,Set (psychology) ,Skepticism ,media_common - Abstract
Despite many attempts to achieve an adequate definition of living systems by means of a set of necessary and sufficient conditions, the opinion that such an enterprise is inexorably destined to fail is increasingly gaining support. However, we believe options do not just come down to either having faith in a future success or endorsing skepticism. In this paper, we aim to redirect the discussion of the problem by shifting the focus of attention from strict definitions (in terms of necessary and sufficient conditions) towards a philosophical framework that allows conceiving of living systems as a natural kind, but whereby natural kinds are not to be defined by fixed necessary and sufficient conditions. We argue for a property-cluster kind approach according to which living systems constitute a natural kind with vague boundaries, capable of changing, and whose members do not need to instantiate every property. We draw from Boyd’s homeostatic property-cluster theory and introduce two modifications, one regarding homeostatic mechanisms and another related to the scientific role of kinds. Thus, our view overcomes some difficulties of Boyd’s theory and we are able to account for the natural kindhood of living things. We also emphasize the most appealing features of our approach for specific research fields and address three objections to this sort of approach.
- Published
- 2018
24. Dudar a dúo. Propuestas teórico-metodológicas para un abordaje del dúo Joaquín Sabina & Benjamín Prado
- Author
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María Julia Ruiz
- Subjects
General Medicine - Abstract
La escritura colaborativa en literatura y, más específicamente, los dúos de escritores, no suelen ser temas de interés científico. Considerados como un ejercicio de estilo, un juego literario o un género menor, la crítica no se ha encargado de indagar en este modo particular de creación poética, aquella que rompe los paradigmas de la genialidad autorial y la supremacía del yo en busca de pluralidad. Nos acercaremos a esta zona de vacancia para sentar las bases teórico-metodológicas que indagarán de qué manera Benjamín Prado y Joaquín Sabina se permiten “dudar a dúo” y operar como una instancia productora de imágenes y posturas de un personaje de autor (en) singular.
- Published
- 2021
25. Secuenciación y evaluación del efecto antimicrobiano de bacterias acidolácticas frente a cepas de Salmonella Tiphymurium
- Author
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Analía Inés Etcheverría, L. M. Medina, María Julia Ruiz, and Rocío Colello
- Subjects
Otras Ingenierías y Tecnologías ,media_common.quotation_subject ,Ciencias Veterinarias ,General Medicine ,Art ,INGENIERÍAS Y TECNOLOGÍAS ,Alimentos y Bebidas ,PEDIOCOCUS ,LACTOBACILLUS ,purl.org/becyt/ford/2 [https] ,CIENCIAS AGRÍCOLAS ,purl.org/becyt/ford/4.3 [https] ,Humanities ,purl.org/becyt/ford/4 [https] ,media_common ,SALMONELLA TYPHIMURIUM ,BIOLOGICAL CONTROL ,purl.org/becyt/ford/2.11 [https] - Abstract
La producción intensiva y extensiva de cerdo en Argentina y España respectivamente presenta factores productivos diferentes. Sin embargo, cuando se trata de amenazas ante patógenos, específicamente Salmonella, ambos se ven afectados. Salmonella es un patógeno común en los alimentos relacionado con las toxiinfecciones alimentarias que provoca graves problemas en la salud pública. Las bacterias acidolácticas, específicamente los géneros Lactobacillus y Pediococcus se han caracterizado en los últimos años por tener gran importancia como antagonistas de diferentes bacterias patógenas. Una alternativa de control es la aplicación de Lactobacillus a lo largo de la cadena productiva de cerdo, evitando así la llegada de este patógeno a los consumidores. Se realizó una evaluación preliminar de la capacidad antagónica de bacterias acidolácticas identificadas tras secuenciación como Lactobacillus plantarum y Pediococcus acidilactici ante Salmonella Tiphymurium aislada de producción intensiva de cerdo de Argentina y Salmonella Tiphymurium aislada de producción extensiva de cerdo en España. Los resultados obtenidos reflejaron una alta capacidad antimicrobiana de las BAL seleccionadas ante ambas cepas patógenas (93.75%). De esta manera, se proponen como potenciales bacterias biopreservadoras en la producción de carne de cerdo y subproductos. The intensive and extensive production of pigs in Argentina and Spain, respectively, present different production factors. However, when dealing with pathogen threats, specifically Salmonella, both are affected by them. Salmonella is a common pathogen in food related to food outbreaks that cause serious problems in public health. Lactic acid bacteria, specifically the genera Lactobacillus and Pediococcus, have been characterized in the past few years for being of great importance as antagonists of different pathogenic bacteria. One control alternative is the application of Lactobacillus throughout the pig production chain, thus preventing the arrival of this pathogen to consumers. A preliminary assessment was made of the antagonistic capacity of lactic acid bacteria identified after sequencing as Lactobacillus plantarum and Pediococcus acidilactici against Salmonella Tiphymurium isolated from intensive pig production in Argentina, and Salmonella Tiphymurium isolated from extensive pig production in Spain. The results obtained showed the high antimicrobial capacity of the LAB selected against both pathogenic strains (93.75%). Thus, they can be proposed as potential biopreservative bacteria in the production of pig meat and its subproducts. Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Medina, Luis Manuel. Universidad de Córdoba. Departamento de Bromatología y Tecnología de los Alimentos; España
- Published
- 2019
26. Env-Specific IgA from Viremic HIV-Infected Subjects Compromises Antibody-Dependent Cellular Cytotoxicity
- Author
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Juliana Falivene, Yanina Alexandra Ghiglione, María Julia Ruiz, María Pía Holgado, Pedro Cahn, Horacio Salomón, María Eugenia Socías, Ana M. Rodríguez, Luis D. Giavedoni, María Magdalena Gherardi, Natalia Laufer, Gabriela Turk, and Omar Sued
- Subjects
Adult ,Male ,0301 basic medicine ,Immunoglobulin A ,Immunology ,HIV Infections ,chemical and pharmacologic phenomena ,Viremia ,HIV Antibodies ,HIV Envelope Protein gp120 ,Microbiology ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Immunity ,Virology ,medicine ,Humans ,Fluorometry ,030212 general & internal medicine ,HIV vaccine ,Young adult ,Aged ,Antibody-dependent cell-mediated cytotoxicity ,biology ,Antibody-Dependent Cell Cytotoxicity ,virus diseases ,hemic and immune systems ,Middle Aged ,medicine.disease ,3. Good health ,Chronic infection ,030104 developmental biology ,Insect Science ,biology.protein ,Pathogenesis and Immunity ,Antibody - Abstract
Elucidating the factors that modulate HIV-specific antibody-dependent cellular cytotoxicity (ADCC) will help in understanding its role in HIV immunity. The aim of this study was to determine whether IgA could modify the magnitude of ADCC in HIV infection, abrogating its protective role. Plasma samples from 20 HIV-positive (HIV + ) subjects enrolled during primary HIV infection (PHI), 10 chronically infected subjects (chronic), and 7 elite controllers (EC) were used. ADCC was determined by using a fluorometric ADCC assay, before and after removal of plasma IgA. Data were analyzed by using nonparametric statistics. ADCC was documented in 80% of PHI enrollment samples and in 100% of PHI 12-month, chronic, and EC samples; it peaked after acute infection, reached a plateau in chronic infection, and decreased after initiation of antiretroviral treatment (ART). Significant associations between ADCC and disease progression were found only after removal of plasma IgA from 12-month PHI samples: the magnitude of ADCC not only increased after IgA removal but also correlated with CD4 + T-cell preservation. This work provides evidence that gp120-specific IgA was capable of modifying ADCC responses during natural HIV infection for the first time and adds to similar evidence provided in other settings. Furthermore, it underscores the complexity of the ADCC phenomenon and will help in an understanding of its underlying mechanisms. IMPORTANCE Although the induction of ADCC-mediating antibodies in HIV-infected subjects has been extensively documented, the association of these antibodies with protection from disease progression is poorly understood. Here, we demonstrate that plasma IgA is a factor capable of modifying the magnitude of IgG-mediated ADCC in HIV infection, mitigating its beneficial effect. These results help in understanding why previous studies failed to demonstrate correlations between ADCC and disease progression, and they also contribute to the notion that an HIV vaccine should stimulate the production of ADCC-mediating IgG antibodies but not IgA.
- Published
- 2016
27. Diferentes métodos para aislamiento y detección de Salmonella spp. en canales porcinas
- Author
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Analía Inés Etcheverría, Nora Lía Padola, Cristina Monteavaro, Rocío Colello, Grisel Ramallo, María Julia Ruiz, and Cristina Villalobo
- Subjects
Salmonella spp. - PCR – Inmunocromatografía - Pruebas bioquímicas ,Ciencias Veterinarias ,General Medicine ,Biochemical tests ,SALMONELLA SPP ,INMUNOCROMATOGRAFIA ,Inmunocromatografía ,PCR ,CIENCIAS AGRÍCOLAS ,Otras Ciencias Veterinarias ,Salmonella spp ,PRUEBAS BIOQUÍMICAS ,Immunochromatography ,purl.org/becyt/ford/4.3 [https] ,purl.org/becyt/ford/4 [https] ,TP248.13-248.65 ,Biotechnology - Abstract
La Organización Mundial de la Salud (OMS) define salmonelosis como una de las enfermedades transmitida por alimentos (ETA) de mayor casuística, ampliamente extendida en todo el mundo. La enfermedad es producida por Salmonella spp. y causa una de las zoonosis más frecuentes y de mayor impacto económico. El hombre adquiere la infección después de la ingestión de alimen-tos contaminados, aunque también puede transmitirse de persona a persona o por vía fecal-oral. Actualmente, las técnicas micro-biológicas de aislamiento convencional para detección de Salmonella spp. son establecidas por el Código Alimentario Argentino para verificar la aptitud de un producto para consumo, pero éstas requieren de 4 a 5 días para la obtención de un resultado, tiem-po que juega en contra para el productor y la conservación de dichos alimentos. Por este motivo en este trabajo se analizan los métodos de diagnóstico tradicional según Normas ISO 6579:2002 con algunas modificaciones, los métodos de inmunoensayo comerciales y la Reacción en Cadena de la Polimerasa técnica (PCR) de detección del gen invA implicado en el proceso de inva-sión de cepas patógenas. Se analizaron 60 muestras procedentes de canales porcinas destinadas a comercialización. Se detectó un 10% de Salmonella spp. Se pudo determinar que el diagnóstico molecular por PCR posee alta sensibilidad, pero no es alenta-dor el resultado que reflejan los test comerciales inmunocromatográficos ya que queda en evidencia la necesidad de alta carga microbiana para un diagnóstico certero. The World Health Organization (WHO) defines salmonellosis as one of the most important foodborne diseases, widely spread worldwide. The disease is produced by Salmonella spp. and causes one of the most frequent zoonoses and of greater economic impact. The infection is acquired after ingestion of contaminated food, although it can also be transmitted from person to person or by fecal-oral route. Currently, conventional isolation microbiological techniques for detection of Salmonella spp. are established by the Argentine Food Code to verify the suitability of a product for consumption. But microbiological techniques require 4 to 5 days to obtain a result, time that plays against the producer and the conservation of such foods. For this reason in this work we analyze the traditional diagnostic methods according to ISO standards 6579: 2002 with some modifications, the commercial immunoassay methods and the Polymerase Chain Reaction technique (PCR) detection of the invA gene involved in the process of invasion of pathogenic strains. Sixty samples from pigs destined for commercialization were analyzed. 10% of Salmonella spp. was detected. It was possible to determine that the molecular diagnosis by PCR has high sensitivity, but it is not encouraging the result that reflect the commercial immunochromatographic tests since it is evident the need of high microbial load for a correct diagnosis. Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Ramallo, Grisel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Villalobo, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Monteavaro, Cristina Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina
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- 2018
28. Interaction between macrophage migration inhibitory factor and CD74 in human immunodeficiency virus type I infected primary monocyte-derived macrophages triggers the production of proinflammatory mediators and enhances infection of unactivated CD4+ T cells
- Author
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Jimena Salido, Yanina Alexandra Ghiglione, César Ariel Trifone, Richard Bucala, María Florencia Quiroga, Gabriela Turk, María Julia Ruiz, Lin Leng, and Horacio Salomón
- Subjects
0301 basic medicine ,Permissiveness ,lcsh:Immunologic diseases. Allergy ,CIENCIAS MÉDICAS Y DE LA SALUD ,CD74 ,IMMUNOPATHOGENESIS ,Immunology ,Inmunología ,primary monocyte-derived macrophages ,CD4+ T-cells ,Proinflammatory cytokine ,03 medical and health sciences ,Immune system ,Immunology and Allergy ,Original Research ,biology ,human immunodeficiency virus ,Chemistry ,immunopathogenesis ,purl.org/becyt/ford/3.1 [https] ,HUMAN IMMUNODEFICIENCY VIRUS ,Medicina Básica ,030104 developmental biology ,PRIMARY MONOCYTE-DERIVED MACROPHAGES ,MACROPHAGE MIGRATION INHIBITORY FACTOR ,TLR4 ,biology.protein ,macrophage migration inhibitory factor ,purl.org/becyt/ford/3 [https] ,Tumor necrosis factor alpha ,Macrophage migration inhibitory factor ,Antibody ,lcsh:RC581-607 ,CD4+ T-CELLS - Abstract
Understanding the mechanisms of human immunodeficiency virus type I (HIV-1) pathogenesis would facilitate the identification of new therapeutic targets to control the infection in face of current antiretroviral therapy limitations. CD74 membrane expression is upregulated in HIV-1-infected cells and the magnitude of its modulation correlates with immune hyperactivation in HIV-infected individuals. In addition, plasma level of the CD74 activating ligand macrophage migration inhibitory factor (MIF) is increased in infected subjects. However, the role played by MIF/CD74 interaction in HIV pathogenesis remains unexplored. Here, we studied the effect of MIF/CD74 interaction on primary HIV-infected monocyte-derived macrophages (MDMs) and its implications for HIV immunopathogenesis. Confocal immunofluorescence analysis of CD74 and CD44 (the MIF signal transduction co-receptor) expression indicated that both molecules colocalized at the plasma membrane specifically in wild-type HIV-infected MDMs. Treatment of infected MDMs with MIF resulted in an MIF-dependent increase in TLR4 expression. Similarly, there was a dose-dependent increase in the production of IL-6, IL-8, TNFα, IL-1β, and sICAM compared to the no-MIF condition, specifically from infected MDMs. Importantly, the effect observed on IL-6, IL-8, TNFα, and IL-1β was abrogated by impeding MIF interaction with CD74. Moreover, the use of a neutralizing αMIF antibody or an MIF antagonist reverted these effects, supporting the specificity of the results. Treatment of unactivated CD4+ T-cells with MIF-treated HIV-infected MDM-derived culture supernatants led to enhanced permissiveness to HIV-1 infection. This effect was lost when CD4+ T-cells were treated with supernatants derived from infected MDMs in which CD74/MIF interaction had been blocked. Moreover, the enhanced permissiveness of unactivated CD4+ T-cells was recapitulated by exogenous addition of IL-6, IL-8, IL-1β, and TNFα, or abrogated by neutralizing its biological activity using specific antibodies. Results obtained with BAL and NL4-3 HIV laboratory strains were reproduced using transmitted/founder primary isolates. This evidence indicated that MIF/CD74 interaction resulted in a higher production of proinflammatory cytokines from HIV-infected MDMs. This caused the generation of an inflammatory microenvironment which predisposed unactivated CD4+ T-cells to HIV-1 infection, which might contribute to viral spreading and reservoir seeding. Overall, these results support a novel role of the MIF/CD74 axis in HIV pathogenesis that deserves further investigation. Fil: Trifone, César Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Salido, Jimena Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Leng, Lin. University of Yale; Estados Unidos Fil: Quiroga, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Salomon, Horacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Bucala, Richard. University of Yale; Estados Unidos Fil: Ghiglione, Yanina Alexandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
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- 2018
29. Detection and characterization of Salmonella Serotypes in the production chain of two pig farms in Buenos Aires Province, Argentina
- Author
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Rocío Colello, Analía Inés Etcheverría, Gerardo Anibal Leotta, Valeria M. Padín, Ariel Diego Rogé, Nora Lía Padola, and María Julia Ruiz
- Subjects
0301 basic medicine ,Microbiology (medical) ,Serotype ,Salmonella ,prevalence ,030106 microbiology ,lcsh:QR1-502 ,Eric pcr ,medicine.disease_cause ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,Otras Ciencias Veterinarias ,MDR ,medicine ,Prevalence ,Pig farms ,pork production chain ,Ciencias Veterinarias ,Pork production chain ,Salmonella serotypes ,food and beverages ,030104 developmental biology ,Geography ,CIENCIAS AGRÍCOLAS ,ERIC-PCR ,purl.org/becyt/ford/4.3 [https] ,Humanities ,purl.org/becyt/ford/4 [https] ,Production chain - Abstract
The aim of the present study was to determine the prevalence of Salmonella in the pork production chain and to characterize Salmonella isolates. From 764 samples, 35 (4.6%) were positive for Salmonella spp., as determined by biochemical tests and the presence of the invA gene. From these, 2.6, 2.0, 8.8, and 8.0% corresponded to samples collected from farms, slaughterhouses, boning rooms and retail markets, respectively. Salmonella strains were classified into five serotypes and distributed as follows: S. Typhimurium in the pork production chain, S. Kentucky in farms and slaughterhouses, S. Brandenburg in slaughterhouses, S. Livingstone in farms and S. Agona in boning rooms and retail markets. Interestingly, the antimicrobial susceptibility testing indicated that all 35 Salmonella spp.-positive isolates were resistant to at least one antimicrobial agent, and 30 were multidrug-resistant (MDR) and resistant to different classes of antibiotics. The enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis showed clonal relatedness among strains isolated from farms, boning rooms and retail markets. The presence of antibiotic-resistant Salmonella in food poses a potential health hazard to consumers., Instituto de Genética Veterinaria
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- 2018
30. Effect of Bismuth Hydroxide Gel on Shiga Toxin Producing Escherichia coli
- Author
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María Emilia Cáceres, Rocío Colello, Analía Inés Etcheverría, María Julia Ruiz, and Nora Lía Padola
- Subjects
Antifungal ,CONTROL ,Endocrine toxicology ,STRATEGIES ,medicine.drug_class ,Food toxicology ,media_common.quotation_subject ,Veterinary toxicology ,BISMUTH HYDROXIDE GEL ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,STEC NON-O157 ,Biología Celular, Microbiología ,FOOD ,medicine ,SHIGA TOXIN ,Industrial toxicology ,purl.org/becyt/ford/1.6 [https] ,Shiga toxin-producing Escherichia coli ,media_common ,Systems toxicology ,Art ,STEC O157 ,HEMOLYTIC UREMIC SYNDROME ,Humanities ,CIENCIAS NATURALES Y EXACTAS - Abstract
Shiga toxin-producing Escherichia coli (STEC) are emerging pathogens associatedwith severe and fatal disease in children as Hemolytic Uremic Syndrome (HUS).These bacteria are shedding with feces of cattle contaminating the environmentand would enter the food chain if the slaughter process is not done correctly.The prevention measures and control strategies are the key tools to reducethe transmission of STEC. Bismuth hydroxide gel has been widely used asantidiarrheal. In this study, the effects of Bismuth hydroxide gel on culture ofSTEC O157:H7, 026:H11 and O103:H2 was assayed. To evaluate the effects onthe viability of STEC O157:H7, O91:H21 and O26:H11 on glass surfaces, twotypes of novel Bismuth hydroxide presentations, emulsion spray and aerosolwere assayed. STEC strains were cultured in LB broth and Bismuth hydroxide gel(Soubeiran Chobet, S.R.L., and City of Buenos Aires, Argentina) was added to eachplate. At different times, an aliquote of each culture were plated onto MacConkeyagar for colony counts. To evaluate the effects on the viability of STEC O157 andnon-O157 strains on glass surfaces, Bismuth hydroxide gel emulsion spray andaerosol was independently sprayed on sterile glass plates previously scatteredwith STEC O157:H7, O91:H21 and O26:H11. The effects were determined atdifferent times by swabbing on MacConkey agar plates and counting CFU. In bothassays, STEC strains without the addition of bismuth hydroxide gel were used ascontrols.All the STEC strains were affected in their growth after the application ofBismuth hydroxide gel in LB broth. Bismuth spray and aerosol were effective forSETC viability on surfaces although the spray showed more efficiency than theaerosol. Since that contaminated surfaces with STEC represent a risk in the foodindustry, Bismuth Hydroxide gel in these novel presentations is promising asdecontaminant on inert surfaces. Fil: Colello, Rocío. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Etcheverría, Analía Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Ruiz, Maria Julia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Cáceres, María Emilia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
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- 2018
31. Biomarkers of progression after HIV acute/early infection: Nothing compares to CD4+ T-cell count?
- Author
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María Magdalena Gherardi, Jimena Salido, María Paula Caruso, María A. Pando, Omar Sued, María Julia Ruiz, Luis D. Giavedoni, Roberto Daniel Rabinovich, María Pía Holgado, Horacio Salomón, Juliana Falivene, Yanina Alexandra Ghiglione, César Ariel Trifone, Macarena Hormanstorfer, Natalia Laufer, Gabriela Turk, Romina Soledad Coloccini, María Inés Figueroa, and Pedro A. Pury
- Subjects
CD4-Positive T-Lymphocytes ,Male ,0301 basic medicine ,soluble plasma factors ,IMMUNE RESPONSES ,lcsh:QR1-502 ,HIV Infections ,lcsh:Microbiology ,purl.org/becyt/ford/1 [https] ,0302 clinical medicine ,Interferon ,Medicine ,030212 general & internal medicine ,Macrophage inflammatory protein ,Interleukin ,Viral Load ,SOLUBLE PLASMA FACTORS ,3. Good health ,HLA ,Infectious Diseases ,Acute Disease ,Cytokines ,Female ,Tumor necrosis factor alpha ,Viral load ,CIENCIAS NATURALES Y EXACTAS ,medicine.drug ,Adult ,Receptors, CCR5 ,BIOMARKERS ,Alpha (ethology) ,Human leukocyte antigen ,Article ,Ciencias Biológicas ,03 medical and health sciences ,disease progression ,Immune system ,Virology ,Humans ,purl.org/becyt/ford/1.6 [https] ,DECISION TREES ,decision trees ,business.industry ,DISEASE PROGRESSION ,biomarkers ,HIV ,immune responses ,CD4 Lymphocyte Count ,Chemokine CXCL10 ,030104 developmental biology ,Immunology ,HIV-1 ,ACUTE INFECTION ,acute infection ,business ,Virología - Abstract
Progression of HIV infection is variable among individuals, and definition disease progression biomarkers is still needed. Here, we aimed to categorize the predictive potential of several variables using feature selection methods and decision trees. A total of seventy-five treatment-naïve subjects were enrolled during acute/early HIV infection. CD4+ T-cell counts (CD4TC) and viral load (VL) levels were determined at enrollment and for one year. Immune activation, HIV-specific immune response, Human Leukocyte Antigen (HLA) and C-C chemokine receptor type 5 (CCR5) genotypes, and plasma levels of 39 cytokines were determined. Data were analyzed by machine learning and non-parametric methods. Variable hierarchization was performed by Weka correlation-based feature selection and J48 decision tree. Plasma interleukin (IL)-10, interferon gamma-induced protein (IP)-10, soluble IL-2 receptor alpha (sIL-2Rα) and tumor necrosis factor alpha (TNF-α) levels correlated directly with baseline VL, whereas IL-2, TNF-α, fibroblast growth factor (FGF)-2 and macrophage inflammatory protein (MIP)-1β correlated directly with CD4+ T-cell activation (p < 0.05). However, none of these cytokines had good predictive values to distinguish “progressors” from “non-progressors”. Similarly, immune activation, HIV-specific immune responses and HLA/CCR5 genotypes had low discrimination power. Baseline CD4TC was the most potent discerning variable with a cut-off of 438 cells/μL (accuracy = 0.93, κ-Cohen = 0.85). Limited discerning power of the other factors might be related to frequency, variability and/or sampling time. Future studies based on decision trees to identify biomarkers of post-treatment control are warrantied. Fil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Ghiglione, Yanina Alexandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Hormanstorfer, Macarena. Fundación Huésped; Argentina Fil: Laufer, Natalia Lorna. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina Fil: Coloccini, Romina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Salido, Jimena Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Trifone, César Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Falivene, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Holgado, María Pía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Caruso, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Figueroa, María Inés. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina. Fundación Huésped; Argentina Fil: Salomon, Horacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Giavedoni, Luis D.. Texas Biomedical Research Institute; Estados Unidos Fil: Pando, María de los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Gherardi, Maria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Rabinovich, Roberto Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Pury, Pedro Angel. Universidad Nacional de Córdoba; Argentina Fil: Sued, Omar Gustavo. Fundación Huésped; Argentina
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- 2018
32. Evaluation of Different Parameters of Humoral and Cellular Immune Responses in HIV Serodiscordant Heterosexual Couples: Humoral Response Potentially Implicated in Modulating Transmission Rates
- Author
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G. Damilano, Natalia Laufer, María Magdalena Gherardi, Gabriela Turk, Jimena Salido, María Julia Ruiz, Omar Sued, Luis D. Giavedoni, Yanina Alexandra Ghiglione, Lorena Abusamra, César Ariel Trifone, Ana M. Rodríguez, Cintia Cevallos, and Horacio Salomón
- Subjects
0301 basic medicine ,Immunoglobulin A ,Chemokine ,HIV-1 transmission ,Otras Ciencias Biológicas ,lcsh:Medicine ,Context (language use) ,General Biochemistry, Genetics and Molecular Biology ,Immunoglobulin G ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,030212 general & internal medicine ,purl.org/becyt/ford/1.6 [https] ,Adcc ,Antibody-dependent cell-mediated cytotoxicity ,lcsh:R5-920 ,biology ,Serodiscordant couples ,lcsh:R ,Hiv-1 ,Hiv-1 Transmission ,virus diseases ,General Medicine ,Virology ,3. Good health ,030104 developmental biology ,Serodiscordant ,Immunology ,HIV-1 ,biology.protein ,Serodiscordant Couples ,Antibody ,ADCC ,lcsh:Medicine (General) ,CIENCIAS NATURALES Y EXACTAS - Abstract
As the HIV/AIDS pandemic still progresses, understanding the mechanisms governing viral transmission as well as protection from HIV acquisition is fundamental. In this context, cohorts of HIV serodiscordant heterosexual couples (SDC) represent a unique tool. The present study was aimed to evaluate specific parameters of innate, cellular and humoral immune responses in SDC. Specifically, plasma levels of cytokines and chemokines, HIV-specific T-cell responses, gp120-specific IgG and IgA antibodies, and HIV-specific antibody-dependent cellular cytotoxicity (ADCC) activity were assessed in nine HIV-exposed seronegative individuals (ESN) and their corresponding HIV seropositive partners (HIV+-P), in eighteen chronically infected HIV subjects (C), nine chronically infected subjects known to be HIV transmitters (CT) and ten healthy HIV− donors (HD). Very low magnitude HIV-specific cellular responses were found in two out of six ESN. Interestingly, HIV+-P had the highest ADCC magnitude, the lowest IgA levels and the highest IgG/IgA ratio, all compared to CT. Positive correlations between CD4+ T-cell counts and both IgG/IgA ratios and %ADCC killing uniquely distinguished HIV+-P. Additionally, evidence of IgA interference with ADCC responses from HIV+-P and CT is provided. These data suggest for the first time a potential role of ADCC and/or gp120-specific IgG/IgA balance in modulating heterosexual transmission. In sum, this study provides key information to understand the host factors that influence viral transmission, which should be considered in both the development of prophylactic vaccines and novel immunotherapies for HIV-1 infection. Fil: Ruiz, María Julia. Fil: Salido, Jimena. Fil: Abusamra, Lorena. Fil: Ghiglione, Yanina. Fil: Cevallos, Cintia. Fil: Damilano, Gabriel. Fil: Rodriguez, Ana María. Fil: Trifone, César Ariel. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina Fil: Laufer, Natalia Lorna. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina Fil: Giavedoni, Luis D.. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina Fil: Sued, Omar. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina Fil: Salomón, Horacio. Fil: Turk, Gabriela Julia Ana.
- Published
- 2017
33. HIV-TB coinfection impairs CD8+T-cell differentiation and function while dehydroepiandrosterone improves cytotoxic antitubercular immune responses
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Pedro Cahn, Patricia Maidana, María Belén Vecchione, Matias Tomas Angerami, Horacio Salomón, Viviana Mesch, María Julia Ruiz, Guadalupe Verónica Suarez, María Florencia Quiroga, Natalia Laufer, Omar Sued, Diego Ameri, Gabriela Turk, Bibiana Fabre, and Oscar Bottasso
- Subjects
Immune system ,Immunology ,Coinfection ,medicine ,Human immunodeficiency virus (HIV) ,Immunology and Allergy ,Cytotoxic T cell ,Biology ,medicine.disease ,medicine.disease_cause ,Virology - Abstract
Fil: Suarez, Guadalupe Veronica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina
- Published
- 2015
34. Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART
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Caroline Dufour, Maria Julia Ruiz, Amélie Pagliuzza, Corentin Richard, Aniqa Shahid, Rémi Fromentin, Rosalie Ponte, Amélie Cattin, Tomas Raul Wiche Salinas, Syim Salahuddin, Teslin Sandstrom, Stephanie Burke Schinkel, Cecilia T. Costiniuk, Mohammad-Ali Jenabian, Petronela Ancuta, Jean-Pierre Routy, Éric A. Cohen, Zabrina L. Brumme, Christopher Power, Jonathan B. Angel, and Nicolas Chomont
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CP: Microbiology ,Biology (General) ,QH301-705.5 - Abstract
Summary: HIV persists in tissues during antiretroviral therapy (ART), but the relative contribution of different anatomical compartments to the viral reservoir in humans remains unknown. We performed an extensive characterization of HIV reservoirs in two men who donated their bodies to HIV cure research and who had been on suppressive ART for years. HIV DNA is detected in all tissues, with large variations across anatomical compartments and between participants. Intact HIV genomes represent 2% and 25% of all proviruses in the two participants and are mainly detected in secondary lymphoid organs, with the spleen and mediastinal lymph nodes harboring intact viral genomes in both individuals. Multiple copies of identical HIV genomes are found in all tissues, indicating that clonal expansions are common in anatomical sites. The majority (>85%) of these expanded clones are shared across multiple tissues. These findings suggest that infected cells expand, migrate, and possibly circulate between anatomical sites.
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- 2023
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35. Correction to: 'Dealing with the changeable and blurry edges of living things: a modified version of property-cluster kinds'
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María Julia Ruiz and Jon Umerez
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World Wide Web ,Philosophy ,Philosophy of science ,Property (philosophy) ,History and Philosophy of Science ,Computer science ,Cluster (physics) ,Internet portal - Abstract
The article “Dealing with the changeable and blurry edges of living things: a modified version of property-cluster kinds”, written by Maria J. Ferreira Ruiz and Jon Umerez, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on June 29, 2018 without open access.
- Published
- 2018
36. Modification of the HIV-specific CD8+ T-cell responses in an elite controller after Chikungunya virus infection
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Héctor Pérez, María Julia Ruiz, Yanina Alexandra Ghiglione, Horacio Salomón, Yamila Martin, César Ariel Trifone, Jimena Salido, Patricia Patterson, Natalia Laufer, and Gabriela Turk
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Epidemiology ,Immunology ,Public Health, Environmental and Occupational Health ,Human immunodeficiency virus (HIV) ,Biology ,medicine.disease_cause ,Virology ,Microbiology ,QR1-502 ,Infectious Diseases ,Control theory ,Chikungunya Virus Infection ,medicine ,Cytotoxic T cell ,Public aspects of medicine ,RA1-1270 - Published
- 2016
37. From Farm to Table: Follow-Up of Shiga Toxin-Producing Escherichia coli Throughout the Pork Production Chain in Argentina
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Nora Lía Padola, María Julia Ruiz, Analía Inés Etcheverría, María Emilia Cáceres, Rocío Colello, and Marcelo E. Sanz
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0301 basic medicine ,Microbiology (medical) ,animal diseases ,prevalence ,030106 microbiology ,lcsh:QR1-502 ,Virulence ,Biology ,medicine.disease_cause ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,fluids and secretions ,STX2 ,Otras Ciencias Veterinarias ,medicine ,characterization ,Food science ,foodborne pathogens ,Escherichia coli ,Shiga toxin-producing Escherichia coli ,Management practices ,Original Research ,pork production chain ,Ciencias Veterinarias ,FOODBORNE PATHOGENS ,food and beverages ,PREVALENCE ,Bacterial adhesin ,STEC ,030104 developmental biology ,CIENCIAS AGRÍCOLAS ,PORK PRODUCTION CHAIN ,Pork meat ,purl.org/becyt/ford/4.3 [https] ,CHARACTERIZATION ,purl.org/becyt/ford/4 [https] ,Production chain - Abstract
Pigs are important reservoirs of Shiga toxin-producing Escherichia coli (STEC). The entrance of these strains into the food chain implies a risk to consumers because of the severity of hemolytic uremic syndrome. This study reports the prevalence and characterization of STEC throughout the pork production chain. From 764 samples, 31 (4.05%) were stx positive by PCR screening. At farms, 2.86% of samples were stx positive; at slaughter, 4.08% of carcasses were stx positive and at boning rooms, 6% of samples were stx positive. These percentages decreased in pork meat ready for sale at sales markets (4.59%). From positive samples, 50 isolates could be characterized. At farms 37.5% of the isolates carried stx1/stx2 genes, 37.5% possessed stx2e and 25%, carried only stx2. At slaughter we detected 50% of isolates positive for stx2, 33% for stx2e, and 16% for stx1/stx2. At boning rooms 59% of the isolates carried stx1/stx2, 14% stx2e, and 5% stx1/stx2/stx2e. At retail markets 66% of isolates were positive for stx2, 17% stx2e, and 17% stx1/stx2. For the other virulence factors, ehxA and saa were not detected and eae gene was detected in 12% of the isolates. Concerning putative adhesins, agn43 was detected in 72%, ehaA in 26%, aida in 8%, and iha in 6% of isolates. The strains were typed into 14 E. coli O groups (O1, O2, O8, O15, O20, O35, O69, O78, O91, O121, O138, O142, O157, O180) and 10 H groups (H9, H10, H16, H21, H26, H29, H30, H32, H45, H46). This study reports the prevalence and characterization of STEC strains through the chain pork suggesting the vertical transmission. STEC contamination originates in the farms and is transferred from pigs to carcasses in the slaughter process and increase in meat pork at boning rooms and sales markets. These results highlight the need to implement an integrated STEC control system based on good management practices on the farm and critical control point systems in the food chain. Fil: Colello, Rocío. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Ruiz, María Julia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Sanz, Marcelo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina Fil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina
- Published
- 2016
38. Th17 and Th17/Treg ratio at early HIV infection associate with protective HIV-specific CD8+ T-cell responses and disease progression
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María Pía Holgado, María Eugenia Socías, Omar Sued, Cynthia Maeto, Luis D. Giavedoni, María Magdalena Gherardi, Yanina Alexandra Ghiglione, Pedro Cahn, Juliana Falivene, Natalia Laufer, Gabriela Turk, María Inés Figueroa, María Julia Ruiz, and Horacio Salomón
- Subjects
Adult ,CIENCIAS MÉDICAS Y DE LA SALUD ,TH 17 ,Human immunodeficiency virus (HIV) ,Ciencias de la Salud ,chemical and pharmacologic phenomena ,HIV Infections ,Biology ,CD38 ,CD8-Positive T-Lymphocytes ,medicine.disease_cause ,Lymphocyte Activation ,Primary HIV infection ,T-Lymphocytes, Regulatory ,Article ,purl.org/becyt/ford/3.3 [https] ,Immunity ,medicine ,Cytotoxic T cell ,Humans ,Lymphocyte Count ,Multidisciplinary ,T REG ,Disease progression ,PRIMARY HIV INFECTION ,hemic and immune systems ,Viral Load ,Lymphocyte Subsets ,3. Good health ,Enfermedades Infecciosas ,Immunology ,HIV-1 ,Disease Progression ,Th17 Cells ,purl.org/becyt/ford/3 [https] ,Viral load ,CD8 - Abstract
The aim of this study was to analyze Th17 and Treg subsets and their correlation with anti-HIV T-cell responses and clinical parameters during (acute/early) primary HIV infection (PHI) and up to one year post-infection (p.i). Samples from 14 healthy donors (HDs), 40 PHI patients, 17 Chronics, and 13 Elite controllers (ECs) were studied. The percentages of Th17 and Treg subsets were severely altered in Chronics, whereas all HIV-infected individuals (including ECs) showed Th17/Treg imbalance compared to HDs, in concordance with higher frequencies of activated CD8+ T-cells (HLA-DR+/CD38+). Better clinical status (higher CD4 counts, lower viral loads and activation) was associated with higher Th17 and lower Treg levels. We found positive correlations between Th17 at baseline and anti-HIV CD8+ T-cell functionality: viral inhibitory activity (VIA) and key polyfunctions (IFN-γ+/CD107A/B+) at both early and later times p.i, highlighting the prognostic value of Th17 cells to preserve an effective HIV T-cell immunity. Th17/Treg ratio and the IL-17 relative mean fluorescence intensity (rMFI of IL-17) were also positively correlated with VIA. Taken together, our results suggested a potential link between Th17 and Th17/Treg ratio with key HIV-specific CD8+ T-cell responses against the infection. Fil: Falivene, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Ghiglione, Yanina Alexandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Laufer, Natalia Lorna. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina Fil: Socías, María Eugenia. Fundación Huésped; Argentina Fil: Holgado, María Pía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Ruiz, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Maeto, Cynthia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Figueroa, María Inés. Fundación Huésped; Argentina Fil: Giavedoni, Luis D.. Texas Biomedical Research Institute; Estados Unidos Fil: Cahn, Pedro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina. Fundación Huésped; Argentina Fil: Salomon, Horacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Sued, Omar Gustavo. Fundación Huésped; Argentina Fil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Gherardi, Maria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
- Published
- 2015
39. Early skewed distribution of total and HIV-specific CD8+ T-cell memory phenotypes during primary HIV infection is related to reduced antiviral activity and faster disease progression
- Author
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María Magdalena Gherardi, Pedro Cahn, Natalia Laufer, Juliana Falivene, María Julia Ruiz, Horacio Salomón, Gabriela Turk, María Eugenia Socías, Yanina Alexandra Ghiglione, Luis D. Giavedoni, and Omar Sued
- Subjects
Reduced Antiviral Activity ,Cellular differentiation ,Programmed Cell Death 1 Receptor ,Epitopes, T-Lymphocyte ,lcsh:Medicine ,HIV Infections ,T-Cell Antigen Receptor Specificity ,CD8-Positive T-Lymphocytes ,Lymphocyte Activation ,Cell-Mediated Immunity ,purl.org/becyt/ford/1 [https] ,Immunophenotyping ,T-Lymphocyte Subsets ,Antiretroviral Therapy, Highly Active ,Cytotoxic T cell ,lcsh:Science ,Multidisciplinary ,Cell Differentiation ,Viral Load ,Phenotype ,Primary Hiv Infection ,Disease Progression ,Cytokines ,Infectious diseases ,Viral load ,CIENCIAS NATURALES Y EXACTAS ,Research Article ,Otras Ciencias Biológicas ,Immunology ,Viral diseases ,Biology ,Microbiology ,Virus ,Ciencias Biológicas ,Immune Activation ,Immune system ,Virology ,Humans ,Viremia ,Cd8+ T-Cell ,purl.org/becyt/ford/1.6 [https] ,Immunity to Infections ,Medicine and health sciences ,lcsh:R ,Immunity ,HIV ,Biology and Life Sciences ,CD4 Lymphocyte Count ,Viral replication ,Case-Control Studies ,lcsh:Q ,Peptides ,Immunologic Memory ,CD8 - Abstract
The important role of the CD8+ T-cells on HIV control is well established. However, correlates of immune protection remain elusive. Although the importance of CD8+ T-cell specificity and functionality in virus control has been underscored, further unraveling the link between CD8+ T-cell differentiation and viral control is needed. Here, an immunophenotypic analysis (in terms of memory markers and Programmed cell death 1 (PD-1) expression) of the CD8+ T-cell subset found in primary HIV infection (PHI) was performed. The aim was to seek for associations with functional properties of the CD8+ T-cell subsets, viral control and subsequent disease progression. Also, results were compared with samples from Chronics and Elite Controllers. It was found that normal maturation of total and HIV-specific CD8+ T-cells into memory subsets is skewed in PHI, but not at the dramatic level observed in Chronics. Within the HIV-specific compartment, this alteration was evidenced by an accumulation of effector memory CD8+ T (TEM) cells over fully differentiated terminal effector CD8+ T (TTE) cells. Furthermore, higher proportions of total and HIV-specific CD8+ TEM cells and higher HIV-specific TEM/(TEM+TTE) ratio correlated with markers of faster progression. Analysis of PD-1 expression on total and HIV-specific CD8+ T-cells from PHI subjects revealed not only an association with disease progression but also with skewed memory CD8+ T-cell differentiation. Most notably, significant direct correlations were obtained between the functional capacity of CD8+ T-cells to inhibit viral replication in vitro with higher proportions of fully-differentiated HIV-specific CD8+ TTE cells, both at baseline and at 12 months post-infection. Thus, a relationship between preservation of CD8+ T-cell differentiation pathway and cell functionality was established. This report presents evidence concerning the link among CD8+ T-cell function, phenotype and virus control, hence supporting the instauration of early interventions to prevent irreversible immune damage. Fil: Ghiglione, Yanina Alexandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Falivene, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Ruiz, Maria Juliz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Laufer, Natalia Lorna. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Socías, María Eugenia. Fundación Huésped; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina Fil: Cahn, Pedro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina. Fundación Huésped; Argentina Fil: Giavedoni, Luis. Southwest National Primate Research Center; Estados Unidos Fil: Sued, Omar Gustavo. Fundación Huésped; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos ; Argentina Fil: Gherardi, Maria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Salomon, Horacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
- Published
- 2014
40. Persistent but dysfunctional mucosal SARS-CoV-2-specific IgA and low lung IL-1β associate with COVID-19 fatal outcome: A cross-sectional analysis
- Author
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Maria Julia Ruiz, Gabriel Siracusano, Andréa Cottignies-Calamarte, Daniela Tudor, Fernando Real, Aiwei Zhu, Claudia Pastori, Claude Capron, Arielle R. Rosenberg, Nigel Temperton, Diego Cantoni, Hanqing Liao, Nicola Ternette, Pierre Moine, Mathieu Godement, Guillaume Geri, Jean-Daniel Chiche, Djillali Annane, Elisabeth Cramer Bordé, Lucia Lopalco, and Morgane Bomsel
- Subjects
SARS-CoV-2 ,COVID-19 ,mucosal immunity ,IgA ,severe infection ,inflammatory cytokine ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The role of the mucosal pulmonary antibody response in coronavirus disease 2019 (COVID-19) outcome remains unclear. Here, we found that in bronchoalveolar lavage (BAL) samples from 48 patients with severe COVID-19-infected with the ancestral Wuhan virus, mucosal IgG and IgA specific for S1, receptor-binding domain (RBD), S2, and nucleocapsid protein (NP) emerged in BAL containing viruses early in infection and persist after virus elimination, with more IgA than IgG for all antigens tested. Furthermore, spike-IgA and spike-IgG immune complexes were detected in BAL, especially when the lung virus has been cleared. BAL IgG and IgA recognized the four main RBD variants. BAL neutralizing titers were higher early in COVID-19 when virus replicates in the lung than later in infection after viral clearance. Patients with fatal COVID-19, in contrast to survivors, developed higher levels of mucosal spike-specific IgA than IgG but lost neutralizing activities over time and had reduced IL-1β in the lung. Altogether, mucosal spike and NP-specific IgG and S1-specific IgA persisting after lung severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) clearance and low pulmonary IL-1β correlate with COVID-19 fatal outcome. Thus, mucosal SARS-CoV-2-specific antibodies may have adverse functions in addition to protective neutralization.HighlightsMucosal pulmonary antibody response in COVID-19 outcome remains unclear. We show that in severe COVID-19 patients, mucosal pulmonary non-neutralizing SARS-CoV-2 IgA persit after viral clearance in the lung. Furthermore, low lung IL-1β correlate with fatal COVID-19. Altogether, mucosal IgA may exert harmful functions beside protective neutralization.
- Published
- 2022
- Full Text
- View/download PDF
41. Early Gag Immunodominance of the HIV-Specific T-Cell Response during Acute/Early Infection Is Associated with Higher CD8 T-Cell Antiviral Activity and Correlates with Preservation of the CD4 TCell Compartment
- Author
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María Julia Ruiz, Romina Soledad Coloccini, Juliana Falivene, Omar Sued, María A. Pando, Pedro Cahn, Natalia Laufer, María Magdalena Gherardi, Gabriela Turk, Yanina Alexandra Ghiglione, Ana M. Rodríguez, María Eugenia Socías, Luis D. Giavedoni, and Horacio Salomón
- Subjects
CD4-Positive T-Lymphocytes ,Nef Protein ,Enzyme-Linked Immunospot Assay ,Immunology ,Molecular Sequence Data ,Human immunodeficiency virus (HIV) ,Argentina ,HIV Infections ,Immunodominance ,Biology ,CD8-Positive T-Lymphocytes ,T cell response ,medicine.disease_cause ,Microbiology ,Polymerase Chain Reaction ,gag Gene Products, Human Immunodeficiency Virus ,Statistics, Nonparametric ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,Biología Celular, Microbiología ,HLA Antigens ,Virology ,anti-Gag response ,primary HIV infection ,medicine ,Cytotoxic T cell ,Humans ,Base sequence ,nef Gene Products, Human Immunodeficiency Virus ,purl.org/becyt/ford/1.6 [https] ,Acute-Phase Reaction ,CTL response ,Cd4 t cell ,Base Sequence ,Sequence Analysis, DNA ,Insect Science ,Pathogenesis and Immunity ,Cytokines ,Enzyme linked immunospot assay ,inhibitory viral activity ,Virología ,Biomarkers ,CIENCIAS NATURALES Y EXACTAS - Abstract
The important role of the CD8 T-cell response on HIV control is well established. Moreover, the acute phase of infection represents a proper scenario to delineate the antiviral cellular functions that best correlate with control. Here, multiple functional aspects (specificity, ex vivo viral inhibitory activity [VIA] and polyfunctionality) of the HIV-specific CD8 T-cell subset arising early after infection, and their association with disease progression markers, were examined. Blood samples from 44 subjects recruited within 6 months from infection (primary HIV infection [PHI] group), 16 chronically infected subjects, 11 elite controllers (EC), and 10 healthy donors were obtained. Results indicated that, although Nef dominated the anti-HIV response during acute/early infection, a higher proportion of early anti-Gag T cells correlated with delayed progression. Polyfunctional HIV-specific CD8 T cells were detected at early time points but did not associate with virus control. Conversely, higher CD4 T-cell set points were observed in PHI subjects with higher HIV-specific CD8 T-cell VIA at baseline. Importantly, VIA levels correlated with the magnitude of the anti-Gag cellular response. The advantage of Gag-specific cells may result from their enhanced ability to mediate lysis of infected cells (evidenced by a higher capacity to degranulate and to mediate VIA) and to simultaneously produce IFN-. Finally, Gag immunodominance was associated with elevated plasma levels of interleukin 2 (IL-2) and macrophage inflammatory protein 1 (MIP-1). All together, this study underscores the importance of CD8 T-cell specificity in the improved control of disease progression, which was related to the capacity of Gag-specific cells to mediate both lytic and nonlytic antiviral mechanisms at early time points postinfection. Fil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologia. Centro Nacional de Referencia del Sida; Argentina Fil: Ghiglione, Yanina Alexandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologia. Centro Nacional de Referencia del Sida; Argentina Fil: Falivene, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologia. Centro Nacional de Referencia del Sida; Argentina Fil: Socias, María Eugenia. Fundación Huésped; Argentina Fil: Laufer, Natalia Lorna. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologia. Centro Nacional de Referencia del Sida; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos; Fil: Coloccini, Romina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologia. Centro Nacional de Referencia del Sida; Argentina Fil: Rodríguez, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral; Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina Fil: Pando, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologia. Centro Nacional de Referencia del Sida; Argentina Fil: Giavedoni, Luis David. Texas Biomedical Research Institute. Southwest National Primate Research Center. Department of Virology and Immunology; Estados Unidos de América; Fil: Cahn, Pedro. Fundación Huésped; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos; Fil: Sued, Omar Gustavo. Fundación Huésped; Argentina Fil: Salomon, Horacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina Fil: Gherardi, Maria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomedicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiologia. Centro Nacional de Referencia del Sida; Argentina
- Published
- 2013
42. Pharmacological Inhibition of PPARy Boosts HIV Reactivation and Th17 Effector Functions, while Preventing Progeny Virion Release and de novo Infection
- Author
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Delphine Planas, Augustine Fert, Yuwei Zhang, Jean-Philippe Goulet, Jonathan Richard, Andrés Finzi, Maria Julia Ruiz, Laurence Raymond Marchand, Debashree Chatterjee, Huicheng Chen, Tomas Raul Wiche Salinas, Annie Gosselin, Eric A. Cohen, Jean-Pierre Routy, Nicolas Chomont, and Petronela Ancuta
- Subjects
hiv-1 ,art ,cd4+ t cells ,th17 ,ppary ,il-21 ,Pathology ,RB1-214 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The frequency and functions of Th17-polarized CCR6+RORyt+CD4+ T cells are rapidly compromised upon HIV infection and are not restored with long-term viral suppressive antiretroviral therapy (ART). In line with this, Th17 cells represent selective HIV-1 infection targets mainly at mucosal sites, with long-lived Th17 subsets carrying replication-competent HIV-DNA during ART. Therefore, novel Th17-specific therapeutic interventions are needed as a supplement of ART to reach the goal of HIV remission/cure. Th17 cells express high levels of peroxisome proliferator-activated receptor gamma (PPARy), a transcriptional factor that represses the transcription of the HIV provirus and the rorc gene, which encodes for the Th17-specific master regulator RORyt/RORC2. Thus, we hypothesized that the pharmacological inhibition of PPARy will facilitate HIV reservoir reactivation while enhancing Th17 effector functions. Consistent with this prediction, the PPARy antagonist T0070907 significantly increased HIV transcription (cell-associated HIV-RNA) and RORyt-mediated Th17 effector functions (IL-17A). Unexpectedly, the PPARy antagonism limited HIV outgrowth from cells of ART-treated people living with HIV (PLWH), as well as HIV replication in vitro. Mechanistically, PPARy inhibition in CCR6+CD4+ T cells induced the upregulation of transcripts linked to Th17-polarisation (RORyt, STAT3, BCL6 IL-17A/F, IL-21) and HIV transcription (NCOA1-3, CDK9, HTATIP2). Interestingly, several transcripts involved in HIV-restriction were upregulated (Caveolin-1, TRIM22, TRIM5α, BST2, miR-29), whereas HIV permissiveness transcripts were downregulated (CCR5, furin), consistent with the decrease in HIV outgrowth/replication. Finally, PPARy inhibition increased intracellular HIV-p24 expression and prevented BST-2 downregulation on infected T cells, suggesting that progeny virion release is restricted by BST-2-dependent mechanisms. These results provide a strong rationale for considering PPARy antagonism as a novel strategy for HIV-reservoir purging and restoring Th17-mediated mucosal immunity in ART-treated PLWH.
- Published
- 2020
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43. Diferentes métodos para aislamiento y detección de Salmonella spp. en canales porcinas
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Maria Julia Ruiz, Grisel Ramallo, Rocío Colello, Cristina Villalobo, Cristina Monteavaro, Analía Etcheverría, and Nora L. Padola
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Salmonella spp. - PCR – Inmunocromatografía - Pruebas bioquímicas ,Biotechnology ,TP248.13-248.65 - Abstract
La Organización Mundial de la Salud (OMS) define salmonelosis como una de las enfermedades transmitida por alimentos (ETA) de mayor casuística, ampliamente extendida en todo el mundo. La enfermedad es producida por Salmonella spp. y causa una de las zoonosis más frecuentes y de mayor impacto económico. El hombre adquiere la infección después de la ingestión de alimentos contaminados, aunque también puede transmitirse de persona a persona o por vía fecal-oral. Actualmente, las técnicas microbiológicas de aislamiento convencional para detección de Salmonella spp. son establecidas por el Código Alimentario Argentino para verificar la aptitud de un producto para consumo, pero éstas requieren de 4 a 5 días para la obtención de un resultado, tiempo que juega en contra para el productor y la conservación de dichos alimentos. Por este motivo en este trabajo se analizan los métodos de diagnóstico tradicional según Normas ISO 6579:2002 con algunas modificaciones, los métodos de inmunoensayo comerciales y la Reacción en Cadena de la Polimerasa técnica (PCR) de detección del gen invA implicado en el proceso de invasión de cepas patógenas. Se analizaron 60 muestras procedentes de canales porcinas destinadas a comercialización. Se detectó un 10% de Salmonella spp. Se pudo determinar que el diagnóstico molecular por PCR posee alta sensibilidad, pero no es alentador el resultado que reflejan los test comerciales inmunocromatográficos ya que queda en evidencia la necesidad de alta carga microbiana para un diagnóstico certero.
- Published
- 2018
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44. Modification of the HIV-specific CD8+ T-cell responses in an elite controller after Chikungunya virus infection
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Yanina Ghiglione, Maria Julia Ruiz, Jimena Salido, César Trifone, Yamila Martin, Patricia Patterson, Héctor Pérez, Horacio Salomon, Gabriela Turk, and Natalia Laufer
- Subjects
Microbiology ,QR1-502 ,Public aspects of medicine ,RA1-1270 - Published
- 2016
- Full Text
- View/download PDF
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