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19. Plasma-Membrane-Localized Transporter NREET1 is Responsible for Rare Earth Element Uptake in Hyperaccumulator Dicranopteris linearis

Author

Zheng, Hong-Xiang, Liu, Wen-Shen, Sun, Dan, Zhu, Shi-Chen, Li, Yang, Yang, Yu-Lu, Liu, Ruo-Rong, Feng, Hua-Yuan, Cai, Xuan, Cao, Yue, Xu, Guo-Hua, Morel, Jean Louis, van der Ent, A., Ma, Lena Q., Liu, Yao-Guang, Rylott, Elizabeth L., Qiu, Rong-Liang, Tang, Ye-Tao, Zheng, Hong-Xiang, Liu, Wen-Shen, Sun, Dan, Zhu, Shi-Chen, Li, Yang, Yang, Yu-Lu, Liu, Ruo-Rong, Feng, Hua-Yuan, Cai, Xuan, Cao, Yue, Xu, Guo-Hua, Morel, Jean Louis, van der Ent, A., Ma, Lena Q., Liu, Yao-Guang, Rylott, Elizabeth L., Qiu, Rong-Liang, and Tang, Ye-Tao

Abstract

Rare earth elements (REEs) are critical for numerous modern technologies, and demand is increasing globally; however,production steps are resource-intensive and environmentally damaging.Some plant species are able to hyper accumulate REEs, and understanding the biology behind this phenomenon could play a pivotal role in developing more environmentally friendly REE recovery technologies. Here, we identified a REE transporter NRAMP REE Transporter 1 (NREET1)from the REE hyper accumulator fern Dicranopteris linearis. AlthoughNREET1 belongs to the natural resistance-associated macrophage protein(NRAMP) family, it shares a low similarity with other NRAMP members.When expressed in yeast, NREET1 exhibited REE transport capacity, but itcould not transport divalent metals, such as zinc, nickel, manganese, or iron.NREET1 is mainly expressed in D. linearis roots and predominantly localized in the plasma membrane. Expression studies in Arabidopsis thaliana revealed that NREET1 functions as a transporter mediating REE uptake and transfer from root cell walls into the cytoplasm. Moreover, NREET1 has a higher affinity for transporting light REEs compared to heavy REEs, which is consistent to the preferential enrichment of light REEs in field-grown D. linearis. We therefore conclude that NREET1 may play an important role in the uptake and consequently hyper accumulation of REEs in D.linearis. These findings lay the foundation for the use of synthetic biology techniques to design and produce sustainable, plant-based REE recovery systems.

Published
2023

21. Ca Minerals and Oral Bioavailability of Pb, Cd, and As from Indoor Dust in Mice: Mechanisms and Health Implications

Author

Li, Hong-Bo, primary, Xue, Rong-Yue, additional, Chen, Xiao-Qiang, additional, Lin, Xin-Ying, additional, Shi, Xiao-Xia, additional, Du, Hai-Yan, additional, Yin, Nai-Yi, additional, Cui, Yan-Shan, additional, Li, Li-Na, additional, Scheckel, Kirk G., additional, Juhasz, Albert L., additional, Xue, Xi-Mei, additional, Zhu, Yong-Guan, additional, and Ma, Lena Q., additional

Published
2022
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33. Ca Minerals and Oral Bioavailability of Pb, Cd, and As from Indoor Dust in Mice: Mechanisms and Health Implications.

Author

Hong-Bo Li, Rong-Yue Xue, Xiao-Qiang Chen, Xin-Ying Lin, Xiao-Xia Shi, Hai-Yan Du, Nai-Yi Yin, Yan-Shan Cui, Li-Na Li, Scheckel, Kirk G., Juhasz, Albert L., Xi-Mei Xue, Yong-Guan Zhu, and Ma, Lena Q.

Subjects
ARSENIC analysis, LEAD analysis, CADMIUM analysis, ARSENIC metabolism, DUST, PHOTON absorptiometry, NEAR infrared spectroscopy, BIOAVAILABILITY, ANIMAL experimentation, CADMIUM, CHOLECALCIFEROL, HEALTH outcome assessment, PARATHYROID hormone, MESSENGER RNA, ENZYME-linked immunosorbent assay, QUALITY assurance, DESCRIPTIVE statistics, RESEARCH funding, DIETARY calcium, MINERALS, BIOLOGICAL assay, POLYMERASE chain reaction, SOLUBILITY, DATA analysis software, MICE, ASPARTIC acid, LEAD, INTESTINES
Abstract

BACKGROUND: Elevating dietary calcium (Ca) intake can reduce metal(loid)oral bioavailability. However, the ability of a range of Ca minerals to reduce oral bioavailability of lead (Pb), cadmium (Cd), and arsenic (As) from indoor dust remains unclear. OBJECTIVES: This study evaluated the ability of Ca minerals to reduce Pb, Cd, and As oral bioavailability from indoor dust and associated mechanisms. METHODS: A mouse bioassay was conducted to assess Pb, Cd, and As relative bioavailability (RBA) in three indoor dust samples, which were amended into mouse chow without and with addition of CaHPO4, CaCO3, Ca gluconate, Ca lactate, Ca aspartate, and Ca citrate at 200–5,000 μg/g Ca. The mRNA expression of Ca and phosphate (P) transporters involved in transcellular Pb, Cd and As transport in the duodenum of mice was quantified using real-time polymerase chain reaction. Serum 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], parathyroid hormone (PTH), and renal CYP27B1 activity controlling 1,25(OH)2D3 synthesis were measured using ELISA kits. Metal(loid) speciation in the feces of mice was characterized using X-ray absorption near-edge structure (XANES) spectroscopy. RESULTS: In general, mice exposed to each of the Ca minerals exhibited lower Pb-, Cd-, and As-RBA for three dusts. However, RBAs with the different Ca minerals varied. Among minerals, mice fed dietary CaHPO4 did not exhibit lower duodenal mRNA expression of Ca transporters but did have the lowest Pb and Cd oral bioavailability at the highest Ca concentration (5,000 μg/g Ca; 51%-95% and 52 -74% lower in comparison with the control). Lead phosphate precipitates (e.g., chloropyromorphite) were observed in feces of mice fed dietary CaHPO4. In comparison, mice fed organic Ca minerals (Ca gluconate, Ca lactate, Ca aspartate, and Ca citrate) had lower duodenal mRNA expression of Ca transporters, but Pb and Cd oral bioavailability was higher than in mice fed CaHPO4. In terms of As, mice fed Ca aspartate exhibited the lowest As oral bioavailability at the highest Ca concentration (5,000 μg/g Ca; 41%-72% lower) and the lowest duodenal expression of P transporter (88% lower). The presence of aspartate was not associated with higher As solubility in the intestine. DISCUSSION: Our study used a mouse model of exposure to household dust with various concentrations and species of Ca to determine whether different Ca minerals can reduce bioavailability of Pb, Cd, and As in mice and elucidate the mechanism(s) involved. This study can contribute to the practical application of optimal Ca minerals to protect humans from Pb, Cd, and As coexposure in the environment. [ABSTRACT FROM AUTHOR]

Published
2022
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