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Raising attentions have focused on how to alleviate greenhouse gas (GHG) emissions from orchard system while simultaneously increase fruit production. Microalgae-based biofertilizer represents a promising resource for improving soil fertility and higher productivity. However, the effects of microalgae application more especially live microalgae on GHG emissions are understudied. In this study, fruit yield and quality, GHG emissions, as well as soil organic carbon and nitrogen fractions were examined in a hawthorn orchard, under the effects of live microalgae-based biofertilizer applied at three doses and two modes. Compared with conventional fertilization, microalgae improved hawthorn yield by 15.7%−29.6% with a maximal increment at medium dose by root application, and significantly increased soluble and reducing sugars contents at high dose. While microalgae did not increase GHG emissions except for nitrous oxide at high dose by root application, instead it significantly increased methane uptake by 1.5−2.3 times in root application. In addition, microalgae showed an increasing trend in soil organic carbon content, and significantly increased the contents of soil dissolved organic carbon and microbial biomass carbon, as well as soil ammonium nitrogen and dissolved organic nitrogen at medium dose with root application. Overall, the results indicated that the live microalgae could be used as a green biofertilizer for improving fruit yield without increasing GHG emissions intensity and the comprehensive greenhouse effect, in particular at medium dose with root application. We presume that if lowering chemical fertilizer rates, application of the live microalgae-based biofertilizer may help to reduce nitrous oxide emissions without compromising fruit yield and quality. [ABSTRACT FROM AUTHOR]

Drought is more frequent and intensified due to global warming. Changed conditions in Beijing‐Tianjin–Hebei region which is drier and warmer than before, make it necessary to investigate various optimized irrigation schemes in the winter wheat production. In this study, the DSSAT–CERES‐Wheat model verified by field experimental data was applied to simulate the yield of winter wheat in Beijing–Tianjin–Hebei region from 2010 to 2069a under RCP8.5 climate scenario. The irrigation schemes were set up by adjusting the irrigation amount and irrigation structure to evaluate their adaptive capacity to climate change. The results showed that the regional average yield reduction rates of potential drought were 81.98% and 78.86% in 2010–2039a and 2040–2069a, which were higher in the north than that in the south. The yield reduction rate of potential drought increased with the decrease of irrigation amount, and the adaptive capacity declined with the decrease of irrigation amount, under the same irrigation structure. When 3‐9‐6 irrigation structure was applied, the regional averages of adaptive capacity to potential drought were 28.30%, 26.23%, and 22.22% in 2010–2039a, 29.00%, 26.67%, and 21.76% in 2040–2069a. The shortage of water resources caused by climate change and the possibility of drought limit the potential yield of winter wheat as high as 80% in this region. Priority shall be given to meeting the water demand in jointing stage and filling stage. Irrigation scheme of 3‐9‐6 structure with 180 mm irrigation amount shall be recommended and its adaptive capacity to climate change is the strongest in the near term and the medium‐term. Even if a further 20% reduction in irrigation is applied (144 mm), the dual goals of reducing yield loss and saving 8.28 × 108 t irrigation water per winter wheat season can be achieved. [ABSTRACT FROM AUTHOR]

Fe-Cr-Ni alloys were widely applied to produce aero-engine, industrial gas turbine and other equipments because of their high temperature strength and toughness and creep resistance. In-situ Al2O3/Fe-Cr-Ni composites were prepared by hot pressing sintering process and in-situ synthesis method. In order to reduce the effect of brittle phase on the properties of composites, the prepared samples were kept in vacuum for 2 h at 1000 ℃. Effect of heat treatment on microstructures and mechanical properties of Al2O3/Fe-Cr-Ni composites at room temperature was investigated by XRD and SEM. The results show that Al2O3/Fe-Cr-Ni composites are mainly composed of Fe-Cr-Ni alloy phase, Fe-Cr phase and Al2O3 ceramic reinforcement phase. The Vickers hardness, bending strength and fracture toughness of the hot pressed sintered samples are 4.16 GPa, 298.31 MPa and 8.04 MPa·m1/2, respectively. Austenitic transformation of Fe-Cr phase in composites results in the reduction of hardness to 2.98 GPa through high-heat treatment at 1000 ℃. The values of bending strength and fracture toughness increase obviously with the increasing of the ductile phase contents in the alloy matrix, which are 459.33 MPa and 12.81 MPa·m1/2, respectively.

The transcription factor SOX9 is critical for prostate development, and dysregulation of SOX9 is implicated in prostate cancer (PCa). However, the SOX9-dependent genes and pathways involved in both normal and neoplastic prostate epithelium are largely unknown. Here, we performed SOX9 ChIP sequencing analysis and transcriptome profiling of PCa cells and determined that SOX9 positively regulates multiple WNT pathway genes, including those encoding WNT receptors (frizzled [FZD] and lipoprotein receptor-related protein [LRP] family members) and the downstream β-catenin effector TCF4. Analyses of PCa xenografts and clinical samples both revealed an association between the expression of SOX9 and WNT pathway components in PCa. Finally, treatment of SOX9-expressing PCa cells with a WNT synthesis inhibitor (LGK974) reduced WNT pathway signaling in vitro and tumor growth in murine xenograft models. Together, our data indicate that SOX9 expression drives PCa by reactivating the WNT/β-catenin signaling that mediates ductal morphogenesis in fetal prostate and define a subgroup of patients who would benefit from WNT-targeted therapy., Introduction SOX9 belongs to the SOX (SRY-related HMG box) family of transcription factors and regulates many developmental processes. SOX9 mutations cause campomelic dysplasia, a disease that is characterized by extreme [...]

Elevated atmospheric CO2 concentrations (eCO2) has become the main feature and cause of global change that could affect crop growth in many aspects, including physiological processes and morphological development in plants and nutrient cycling and nutrient uptake from the soil. Studying the responses of crop growth to different nitrogen (N) supply forms under elevated atmospheric CO2 concentrations can guide nutrient management strategies for agricultural production under future climate change scenarios. Few studies addressed the effect of eCO2 on N uptake and morphological development for plants. This study was conducted in the CO2-controlled light incubators based on the sand-pot incubation using wheat and maize as experimental plants. Six treatments were set with two different environmental CO2 concentrations (aCO2, 390 µmol mol−1; eCO2, 690 µmol mol−1) and three different N supply forms, including ammonium−N, nitrate−N and ammonium-nitrate with 1:1 ratio. The following results were obtained: (i) Wheat and maize seedlings, as nitrate-preferring crops, grew better under mixed N forms than under single N forms. For the single N supply treatment, seedlings with nitrate−N supply showed better growth than with ammonium. (ii) For wheat plants, seedlings with a single ammonium−N supply showed slender height and fewer tillers; seedlings with a single nitrate−N supply were characterized by slightly shorter plant height, more tillers, and higher aboveground biomass. (iii) Compared to the aCO2 group, wheat seedlings with the ammonium−N supply showed an increased maximum root length and a decreased carbon concentration in root exudates; wheat seedlings with a single nitrate−N supply under eCO2 showed a significant increase in biomass and a decreased carbon concentration in root exudates; wheat seedlings with a mixed N supply under eCO2 explored a significant increase in carbon concentration in root exudate and a relatively lower N concentration. (iv) For maize plants, seedlings with either single ammonium−N or nitrate−N supply did not show significant differences in most growing indices. Maize seedlings with a mixed N supply exhibited an increase in aboveground biomass and N concentration in root exudates compared to those with a single N supply. (v) Compared with the aCO2 group, maize seedlings with mixed N supply under eCO2 conditions exhibited significant increases in plant height, aboveground biomass, and N concentration in root exudates. Single ammonium−N supply was toxic to wheat and maize plants under eCO2 conditions. We recommend raising the ratio of ammonium-to-nitrate under mixed N supply to improve the coordination of carbon and N metabolism for efficient crop growth under climatic change conditions of elevated atmospheric CO2 concentration. [ABSTRACT FROM AUTHOR]

Fusion of the androgen receptor-regulated (AR-regulated) TMPRSS2 gene with ERG in prostate cancer (PCa) causes androgen-stimulated overexpression of ERG, an ETS transcription factor, but critical downstream effectors of ERG-mediating PCa [...]

Human papillomavirus (HPV) is an important etiological factor of head and neck squamous cell carcinoma (HNSCC). HPV+ HNSCC patients usually have a better prognosis, which probably results from the higher infiltration of B lymphocytes. This study was purposed to detect the infiltration of B lymphocyte subsets and the correlation between B lymphocyte subsets and the prognosis in HPV‐related HNSCC. In this study, 124 HPV+ and 513 HPV− HNSCC samples were obtained from the Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database for transcriptomic analysis. Infiltration of B lymphocytes subsets was detected with 7 HPV+ HNSCC and 13 HPV− HNSCC tissues through immunohistochemistry and immunofluorescence. One HPV− HNSCC sample was detected with single‐cell sequencing for chemokine analysis. In the results, the infiltration of plasma cells (CD19+CD38+) and memory B cells (MS4A1+CD27+) was higher in HPV+ HNSCC samples. High infiltration of plasma cells and memory B cells was related to a better prognosis. High density of B lymphocytes was positively correlated with high CXCL13 production mainly from CD4+ T lymphocytes in HNSCC. These results indicated that a high density of plasma cells and memory B cells could predict excellent prognosis. CD4+ T lymphocytes might affect B lymphocytes and their subsets through the CXCL13/CXCR5 axis in HNSCC. [ABSTRACT FROM AUTHOR]

The previously generated recombinant human (rh) interferon (IFN)-λ1 protein has a short half-life, and this feature makes it challenging to conduct studies on potential clinical applications for rhIFN-λ1. In an attempt to overcome this difficulty, we constructed a 'long-life' version of rhIFN-λ1. This modified rhIFN-λ1, named rhIFN-λ1-CTPON, has a human chorionic gonadotropin β subunit carboxyl-terminal peptide (CTP) and an N-glycosylation sequence linked to its C-terminus. We confirmed the sequence of rhIFN-λ1-CTPON by mass spectrometry and then measured its biological activities. The results show that rhIFN-λ1-CTPON had antiviral activity and anti-proliferation activity in vitro that were similar to those of rhIFN-λ1 and that it similarly promoted natural killer cell cytotoxicity. Notably, the in vivo half-life of rhIFN-λ1-CTPON was determined to be 3-fold higher than that of rhIFN-λ1. We also assessed the anti-hepatitis B virus activity of rhIFN-λ1-CTPON; it was able to inhibit the production of the antigens HBs-Ag and HBe-Ag and induce antiviral gene expression. In conclusion, rhIFN-λ1-CTPON has a longer half-life than rhIFN-λ1 and has similar biological activities, so rhIFN-λ1-CTPON is an appropriate substitute for rhIFN-λ1 in the further study of potential clinical applications for rhIFN- λ1. [ABSTRACT FROM AUTHOR]