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Appropriate harvesting management is essential for sustainable forage production and improving nutritional quality. In this study, four forage crops, including Medicago sativa (Ms), Vicia sativa (Vs), Avena sativa (As), and Sorghum Sudangrass (Ss) were evaluated under two harvesting modes, i.e. single harvest and multiple cuttings. Results showed that multiple cuttings increased the forage yield of Ms., As, and Ss by 82 %–114 %, 7.5 %–51 %, and 21 %–150 % compared to the single harvest. The acid and neutral detergent fiber concentration decreased, while crude protein contents increased with multiple cuttings. Also, multiple cuttings increased the ether extract content of Ms. and Ss and reduced the ash content by 9.42 % to 33.04 % from 2015 to 2016. The variation range of legume forages' annual yield was 18.66 %–33.04 %, while that of gramineous forages was 9.42 %–25.98 %. The relative feeding value (RFV) of Ms. was higher than that of other forages under multiple cuttings. In general, multiple cuttings decreased the SOC content, but the effect was insignificant for the two leguminous forages. Correlations analysis showed a significant positive relationship of RFV and CP with precipitation while a negative relationship of ADF and NDF with temperature. Moreover, results of yield stability, contribution rate, and TOPSIS (Technique for Order Preference by Similarity to an Ideal Solution) analysis showed that the response of legume crops to multiple cutting was superior to that of gramineous crops. Overall, the cultivation of leguminous forage under multiple cuttings can provide a basis for the development of pastoral agriculture production and animal husbandry in arid regions of China.

Melanoma is one of the highly malignant tumors whose incidence and fatality rates have been increased year by year. However, in addition to early surgical resection, there still lacks specific targeted drugs and treatment strategies. In this study, it was discovered that hinokiflavone (HF) encapsulated in zeolitic imidazolate framework-8 (ZIF-8) exhibited a superior anti-melanoma effect in vitro and in vivo. HF was encapsulated in ZIF-8 through a one-step synthesis method, and polyethylene glycol (PEG-2000) was used to optimize the size and dispersion of the drug-loaded complex (PEG/ZIF-8@HF). The results show that the prepared PEG/ZIF-8@HF has a high encapsulation efficiency (92.12%) and can achieve selective drug release in an acidic microenvironment. The results of in vitro anti-melanoma experiments indicate that PEG/ZIF-8@HF shows up-regulation of reactive oxygen species (ROS) levels and can restrain the migration and invasion of B16F10 cells. Moreover, in vivo animal experiments further confirm that PEG/ZIF-8@HF shows anti-tumor effect by up-regulating the pro-apoptotic proteins caspase-3 and caspase-8, and down-regulating the migration-promoting invasion protein MMP-9. This study developed a safe and effective oral administration of HF based on the high-efficiency delivery ZIF-8 system, which provides an effective treatment strategy for melanoma.

Increasing forage yield and nutritional quality under the premise of maintaining relatively stable land area and soil nutrient content is a necessary condition for the sustainable development of grassland animal husbandry. Different cutting models [simulated grazing (SG), hay harvesting (H)] of oat (Avena sativa), common vetch (Vicia sativa) and their mixture (Avena sativa + Vicia sativa) were studied on the Loess Plateau. The results show that (1) SG could increase forage yield, crude protein, and crude fat content and decrease crude ash content. In 2014, the yield of Avena sativa per hectare was 3,578.11 kg higher than that of H; (2) the model analysis for predicting nutritional components showed that the Crude protein (CP) and EE contents of forages in each variety (combination) showed a linear downward trend with increasing forage yield. Redundancy analysis showed that precipitation, especially in the growing season, was positively correlated with grass yield and CP content; and (3) there were significant differences in soil organic carbon, total nitrogen, NO3−-N, and NH4+-N contents for the different forage varieties (combinations) under different use modes; the values first decreased, then increased, and finally decreased. According to the comprehensive evaluation value calculated by Technique for Order Preference by Similarity to an Ideal Solution, mixed sowing was better than monoculture, and SG obtained better results than H. Overall, mixed sowing under SG can improve forage yield and nutritional quality. At the same time, precipitation regulation is the key factor affecting the production performance of rainfed cultivated grassland on the Loess Plateau.