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146 results on '"Lu, Xueqin"'

35. Roles of colloidal particles and soluble biopolymers in long-term performance and fouling behaviors of submerged anaerobic membrane bioreactor treating methanolic wastewater

Author

Lu, Xueqin, primary, Zheng, Chaoting, additional, Zhen, Guangyin, additional, Tan, Yujie, additional, Zhou, Yuhan, additional, Zhang, Zhongyi, additional, Niu, Chengxin, additional, Li, Wanjiang, additional, Kudisi, Dilibaierkezi, additional, Wang, Yue, additional, and Li, Yu-You, additional

Published
2021
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36. Molecular cloning and expression analysis of seven sucrose synthase genes in bamboo (Bambusa emeiensis): investigation of possible roles in the regulation of cellulose biosynthesis and response to hormones

Author

Xu Gang, Huang Yan, Long Zhijian, Ying Cao, Lu Xueqin, Hu Shanglian, and Liao Qian

Subjects
0106 biological sciences, 0301 basic medicine, 2,6-DCB, lcsh:Biotechnology, Biology, Molecular cloning, 01 natural sciences, Isozyme, 03 medical and health sciences, chemistry.chemical_compound, lcsh:TP248.13-248.65, Gene, Abscisic acid, sucrose synthase, chemistry.chemical_classification, Methyl jasmonate, ATP synthase, 030104 developmental biology, Enzyme, Bambusa emeiensis, chemistry, Biochemistry, expression pattern, biology.protein, Sucrose synthase, cellulose biosynthesis, 010606 plant biology & botany, Biotechnology
Abstract

Sucrose synthase (SUS, EC 2.4.1.13) is a key enzyme involved in sucrose metabolism. In this study, amplification by polymerase chain reaction confirmed that there are at least seven SUS genes (BeSUS1-7) in the Bambusa emeiensis genome. The expression patterns of the BeSUSs genes differed from each other in various bamboo tissues. Among these BeSUSs, BeSUS2 was expressed predominately in the root and BeSUS5 was most abundant in developing shoots, indicating that these two isozymes might play more important physiological roles in root and shoot than the other BeSUSs genes, respectively. In addition, BeSUSs response to abscisic acid (ABA), methyl jasmonate (MeJA) and 2,6-dichlorobenzonitrile treatment (2,6-DCB, an inhibitor of cellulose synthesis) was also investigated in leaves. The results showed that the expression levels of the BeSUS1, 5 and 7 genes were not only conspicuously induced by ABA treatment but also up-regulated by exogenous application of MeJA, indicating that these three isozymes are most likely involved in ABA and MeJA stress responses and are important in meeting the increased glycolytic demand that occurs during these stresses. Moreover, after 2,6-DCB treatment, the cellulose content of leaves was decreased and the transcripts of BeSUS2, 3 and 7 were markedly decreased, while those of BeSUS 5 and 6 were conspicuously induced. The results suggest that the possible roles of BeSUSs genes in the pathway of sucrose metabolism, bamboo resistance to abiotic stresses and the regulation of cellulose biosynthesis may be divergent.

Published
2017

44. In vitro callus induction and plant regeneration from mature seed embryo and young shoots in a giant sympodial bamboo, Dendrocalamus farinosus (Keng et Keng f.) Chia et H.L. Fung

Author

Ke Chen, Duan Ning, Jian-ying Zhou, Ren Peng, Hu Shanglian, Lu Xueqin, and Cao Ying

Subjects
Bamboo, fungi, food and beverages, Embryo, Biology, Applied Microbiology and Biotechnology, Sympodial, Callus culture, plant regeneration, making-pulp use, Dendrocalamus farinosus, chemistry.chemical_compound, Basal shoot, Murashige and Skoog medium, chemistry, Callus, Botany, Shoot, Genetics, Kinetin, Agronomy and Crop Science, Molecular Biology, Biotechnology
Abstract

The method for callus induction, adventitious shoot induction and plant regeneration derived from mature embryos of the seed and young shoots in Dendrocalamus farinosus was examined. For young shoots, the lowest callus induction frequency (21.0 to 29.7%) was obtained and the induced callus became brown and perished after two weeks. For mature embryos of the seed, an efficient protocol for callus induction, adventitious shoot induction and plant regeneration was developed. The best callus induction medium for mature embryos was observed to be Murashige and Skoog (MS) supplemented with 2.0 mg l -1 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) in combination with 0.2 mg l -1 kinetin (Kn) plus 0.4 mg l -1 indole-3-butyric acid (IBA). Callus induction frequency was 95%. The highest adventitious shoot induction frequency (91.2 ± 2.18%) was obtained on MS medium supplemented with 2.5 mg l -1 kn plus 0.5 mg l -1 indole-3-acetic acid (IAA). The regenerated adventitious shoots were rooted in vitro on MS medium with 0.4 mg l -1 IBA plus 0.25 mg l -1 IAA. Rooted plantlets successfully acclimatized to the greenhouse and 90.1% survived after being transplanted to plastic pots (measuring 30 cm in diameter) with garden soil. Key words : Callus culture, plant regeneration, making-pulp use, Dendrocalamus farinosus.

Published
2011

45. Continuous micro-current stimulation to upgrade methanolic wastewater biodegradation and biomethane recovery in an upflow anaerobic sludge blanket (UASB) reactor

Author

Zhen, Guangyin, primary, Lu, Xueqin, additional, Kobayashi, Takuro, additional, Su, Lianghu, additional, Kumar, Gopalakrishnan, additional, Bakonyi, Péter, additional, He, Yan, additional, Sivagurunathan, Periyasamy, additional, Nemestóthy, Nándor, additional, Xu, Kaiqin, additional, and Zhao, Youcai, additional

Published
2017
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50. Molecular cloning and expression analysis of seven sucrose synthase genes in bamboo (<italic>Bambusa emeiensis</italic>): investigation of possible roles in the regulation of cellulose biosynthesis and response to hormones.

Author

Huang, Yan, Liao, Qian, Hu, Shanglian, Cao, Ying, Xu, Gang, Long, Zhijian, and Lu, Xueqin

Subjects
MOLECULAR cloning, GENE expression, SUCROSE synthase, BIOSYNTHESIS, POLYMERASE chain reaction, ABSCISIC acid
Abstract

Sucrose synthase (SUS, EC 2.4.1.13) is a key enzyme involved in sucrose metabolism. In this study, amplification by polymerase chain reaction confirmed that there are at least seven SUS genes (BeSUS1-7) in the Bambusa emeiensis genome. The expression patterns of the BeSUSs genes differed from each other in various bamboo tissues. Among these BeSUSs, BeSUS2 was expressed predominately in the root and BeSUS5 was most abundant in developing shoots, indicating that these two isozymes might play more important physiological roles in root and shoot than the other BeSUSs genes, respectively. In addition, BeSUSs response to abscisic acid (ABA), methyl jasmonate (MeJA) and 2,6-dichlorobenzonitrile treatment (2,6-DCB, an inhibitor of cellulose synthesis) was also investigated in leaves. The results showed that the expression levels of the BeSUS1, 5 and 7 genes were not only conspicuously induced by ABA treatment but also up-regulated by exogenous application of MeJA, indicating that these three isozymes are most likely involved in ABA and MeJA stress responses and are important in meeting the increased glycolytic demand that occurs during these stresses. Moreover, after 2,6-DCB treatment, the cellulose content of leaves was decreased and the transcripts of BeSUS2, 3 and 7 were markedly decreased, while those of BeSUS 5 and 6 were conspicuously induced. The results suggest that the possible roles of BeSUSs genes in the pathway of sucrose metabolism, bamboo resistance to abiotic stresses and the regulation of cellulose biosynthesis may be divergent. [ABSTRACT FROM AUTHOR]

Published
2018
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