1. Human S100A10 plays a crucial role in the acquisition of the endometrial receptivity phenotype
- Author
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Samir Hamamah, Laurent Tiers, Hélène Perrochia, Laurence Bissonnette, Isaac Jacques Kadoch, Christophe Hirtz, Delphine Haouzi, Loubna Drissennek, François Bissonnette, Sylvain Lehmann, Yannick Antoine, Développement embryonnaire précoce humain et pluripotence EmbryoPluripotency (UMR 1203), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), OVO Fertility, Plateforme de Protéomique Clinique, CHU Saint-Eloi, Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Hôpital Arnaud de Villeneuve [CHRU Montpellier], and This work was partially supported by a grant from the Ferring Pharmaceutical Company and FEDER (Fonds Européen deDéveloppement Régional).
- Subjects
Proteomics ,0301 basic medicine ,Angiogenesis ,endometrial receptivity ,[SDV]Life Sciences [q-bio] ,Apoptosis ,Endometrium ,Mass Spectrometry ,Extracellular matrix ,Transcriptome ,0302 clinical medicine ,Cell Movement ,Pregnancy ,Annexin A2 ,Cells, Cultured ,030219 obstetrics & reproductive medicine ,S100 Proteins ,early implantation ,Leukocyte extravasation ,Trophoblasts ,Phenotype ,medicine.anatomical_structure ,Gene Knockdown Techniques ,Female ,Research Paper ,Adult ,medicine.medical_specialty ,Stromal cell ,Down-Regulation ,Biology ,proteome ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Internal medicine ,Cell Adhesion ,medicine ,Humans ,Gene silencing ,Embryo Implantation ,Gene Silencing ,human ,Cell Shape ,Menstrual Cycle ,Cell Proliferation ,Decidualization ,Epithelial Cells ,Cell Biology ,030104 developmental biology ,Endocrinology ,S100A10 ,Cancer research ,Stromal Cells - Abstract
International audience; In assisted reproduction, about 30% of embryo implantation failures are related to inadequate endometrial receptivity. To identify molecules involved in endometrial receptivity acquisition, we investigated, using a SELDI-TOF approach, the protein expression profile of early-secretory and mid-secretory endometrium samples. Among the proteins upregulated in mid-secretory endometrium, we investigated the function of S100A10 in endometrial receptivity and implantation process. S100A10 was expressed in epithelial and stromal cells of the endometrium of fertile patients during the implantation windows. Conversely, it was downregulated in the mid-secretory endometrium of infertile patients diagnosed as non-receptive. Transcriptome analysis of human endometrial epithelial and stromal cells where S100A10 was silenced by shRNA revealed the deregulation of 37 and 256 genes, respectively, related to components of the extracellular matrix and intercellular connections. Functional annotations of these deregulated genes highlighted alterations of the leukocyte extravasation signaling and angiogenesis pathways that play a crucial role during implantation. S100A10 silencing also affected the migration of primary endometrial epithelial and stromal cells, decidualization and secretory transformation of primary endometrial stromal cells and epithelial cells respectively, and promoted apoptosis in serum-starved endometrial epithelial cells. Our findings identify S100A10 as a player in endometrial receptivity acquisition.
- Published
- 2015
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