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Additional file 3: Supplementary Table S3. The genome properties and statistics of Citrobacter sp. XT1-2-2

Additional file 4: Supplementary Table S4. Basic information of sulfate reduction pathwaygenes on Chromosomal and BLASTP analysis in swissprot database.

Additional file 7: Supplementary Table S7. Basic information of the Citrobacter species used for the pan genome analysis.

Additional file 5: Supplementary Table S5. Basic information of heavy metal resistancegenes on Chromosomal and BLASTP analysis in swissprot database.

Additional file 2: Supplementary Table S2. Project information of Citrobacter sp. XT1-2-2.

Additional file 11: Supplementary Table S11. Classification of the heavy metal resistancegenes in the gene category.

Additional file 1: Table S1. Strains and plasmids used in this study. Table S2. Nucleotide sequences of primers. Figure S1. Construction and verification of S. spinosa-ΔacuC. Figure S2. Construction and verification of S. spinosa-acuC. Figure S3. The insecticidal activity of the wild-type and mutant strains on H. armigera. Figure S4. The content of Acetyl-CoA between the wild-type and overexpression strains in 48 h, 96 h and 192 h. Figure S5. The acetate content remained in bacteria between the wild-type and overexpression strains during fermentation. Figure S6. The consumption curve of glucose content in the fermentation medium during the growth process. Figure S7. The SDS-PAGE analysis of whole-cell proteins of the wild-type and mutant strains at 48 h. Figure S8. Comparison of acuC protein sequences among different bacteria.

Additional file 1: Table S1. Strains, plasmids and primers used in this study. Table S2. qRT-PCR primers used in this study. Table S3. Biological insecticidal activity of wt, Δbfr and ::bfr. Figure S1. LC-MS/MS identification of butenyl-spinosyn. Figure S2. Phylogenetic tree analysis of bacterioferritin. Figure S3. Construction and recombination schematic diagram of pKCcas9dO-sgRNA-UHA-DHA and pOJ260-kasOp*-bfr. Figure S4. Identification of Δbfr and ::bfr. Figure S5. Tricine-SDS-PAGE analysis and 1D-LC-MS/MS identification of heterologously expressed protein Bfr. Figure S6. Butenyl-spinosyn yield curve of the wild-type and mutants. Figure S7. Statistics of total protein and differential protein between the wild-type and mutant strains identified in the quantitative proteome. Figure S8. The expression fold change of bus family proteins between bfr mutant strains and wild-type strain. Figure S9. Verification of the transcription levels of iron homeostasis and oxidative stress-related proteins.