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With the advent of the Internet era and the improvement of people's living standards, more and more garbage is generated per capita. In order to explore the market-oriented operation of garbage sorting in China, this paper takes universities as an example and creates the "Internet+standardized" university garbage sorting market-oriented operation alliance to test the effect of garbage recycling projects in universities. In the initial stage of the project, the unique conditions such as strong environmental awareness, huge amount of domestic waste, high recyclable rate and regular work and rest are the inherent advantages for promoting waste classification reform. In the implementation process, this paper will establish an integrated platform benefiting schools, students, merchants and recycling enterprises, form a market-based mechanism with a multi-body linkage for resource recovery and reuse, raise the environmental awareness of college students, and explore a suitable path for waste separation to be implemented in China.

With the advent of the Internet era and the improvement of people's living standards, more and more garbage is generated per capita. If backward countries can realize garbage recycling, they can turn waste into treasure, which can not only reduce environmental pollution but also avoid resources of waste. The "Internet + Standardization" university garbage classification market-oriented operation alliance came into being. It uses the "Internet" as a technical means, based on standardized garbage classification, and uses a new market mechanism in various colleges and universities to encourage college students to take the initiative to classify garbage and improve college students. Environmental awareness. During the implementation process, we will establish an integrated platform that benefits schools, students, merchants and recycling enterprises, form a market-oriented mechanism of multi-subject linkage of resource recycling and reuse, improve the environmental protection awareness of college students, and explore a road for garbage classification suitable for implementation in China.

The large conjugated system and the d orbitals of metallic phthalocyanines (MPcs) are easily triggered by solvent molecules and light to cause variations in photo-physical and photo-chemical properties. To clarify the novel properties of photo-excited MPcs in solvents, the steady/transient state spectral data were collected to investigate four MPc templates, including FePc, ZnPc, CoPc and AlClPc. The Q bands of FePc and CoPc were prone to redshift, while the peaks of ZnPc and AlClPc tended to blueshift in various solvents compared with that in DMSO. With xenon lamp irradiation, the characteristic absorption peak intensity of FePc and ZnPc in DMSO decreased gradually and then increased after being subjected to dark condition. The transient absorption spectra and kinetic data illustrated that photo-excited FePc and CoPc produced relatively short lifetime transient intermediates. The positive absorption at 650 nm in the Q bands of FePc and CoPc could be assigned to the d-π* electron transition in transient intermediates with unpaired d-orbital electron in the open shell configuration of Fe

This article presents the experimental characterization of SiC MOSFETs exposed to heavy-ion irradiation. Different leakage paths related to the drain bias used during the tests are observed, suggesting different damage sites in the devices, which can be further verified through the post-irradiation measurements. TCAD simulations are utilized to explore the underlying failure mechanisms. Based on the evolution of the gate oxide field, it is shown that the damage first occurs in the middle of the JFET region, while gradually spreads to the channel region with the increase of drain bias, but terminates at the source region. A preliminary model to roughly emulate the drain bias dependence of such degradation behaviors is developed, and the simulation results match the experiments well. Additionally, the emission microscope (EMMI) is carried out so as to detect the latent damage at the die level. The findings in this article demonstrate that more attention should be paid to the damaged gate oxide induced by the heavy-ion strike before SiC MOSFETs could act as a drop-in replacement of Si-based counterparts in avionic applications.

International audience; Pepper (Capsicum spp.) is an important vegetable crop that provides a unique pungent sensation when eaten. Through construction of a pepper variome map, we examined the main groups that emerged during domestication and breeding of C. annuum, their relationships and temporal succession, and the molecular events underlying the main transitions. The results showed that the initial differentiation in fruit shape and pungency, increase in fruit weight, and transition from erect to pendent fruits, as well as the recent appearance of large, blocky, sweet fruits (bell peppers), were accompanied by strong selection/fixation of key alleles and introgressions in two large genomic regions. Furthermore, we identified Up, which encodes a BIG GRAIN protein involved in auxin transport, as a key domestication gene that controls erect vs pendent fruit orientation. The up mutation gained increased expression especially in the fruit pedicel through a 579bp sequence deletion in its 5 0 upstream region, resulting in the phenotype of pendent fruit. The function of Up was confirmed by virus-induced gene silencing. Taken together, these findings constitute a cornerstone for understanding the domestication and differentiation of a key horticultural crop.

Previous studies indicate that individuals’ default behaviors on online peer-to-peer (P2P) lending platforms greatly influence other borrowers’ default intentions. However, the mechanism of this impact is not clear. Moreover, there is scarce research in regard to which factors influence the relationship between an individual’s default behavior and an observer’s default intention. These important questions are yet to be resolved; hence, we conducted two experiments using the scenario-based research method, focusing on Chinese online P2P lending platforms. Our results indicate that an individual’s default behavior can trigger an observer’s default intention as a result of the imperfect punitive measures as they currently exist on Chinese online P2P lending platforms. Both the observer’s moral disengagement level and pragmatic self-activation level serve as mediating variables. In situations where an observer knows an individual’s default behavior, the level of intimacy between the defaulter and observer positively affects the relationship between their default behavior and intention. The intimacy level also positively influences the relationship between the individual’s default behavior and the two mediator variables. Based on the findings, we provide management suggestions in the context of online P2P lending. Our study sets a foundation for future research to utilize other methods to extend the present research findings to other regions and domains.

Changes in the dissolved oxygen concentration of the ocean have important implications for marine ecosystems and global climate change. However, limited by measurement techniques, the hydrology data is not always complete. Thus, accurate prediction on marine dissolved oxygen concentration (MDOC), is a powerful supplement to the current observation data. Deep neural network is a powerful model to do the prediction, while it is usually difficult and time-consuming to tune its structure. Meanwhile, deep jointly informed neural network (DJINN) provides a user friendly method to tune the structure of neural networks. In this article, a deep learning-based model called marine deep jointly informed neural network (M-DJINN), is proposed to predict MDOC. M-DJINN improves DJINN performance via initializing the weights of neural network using a zero-mean Gaussian distribution with a variance related to the number of neurons in the neighbor layer. In M-DJINN, to get an ideal deep neural network structure, users only need to tune the tree number and max tree depth. While predicting MDOC on World Ocean Database 2013(WOD13) data with M-DJINN, the novel model proves better than DJINN on both accuracy and convergency. When the max tree depth is set to 10, the mean squared error (MSE) is reduced by 17.6% compared with DJINN. It can be concluded from the experiments that with enough training data, the performance of M-DJINN may keep improving by deepening the neural networks.

We present a new magnetometry method integrating an ensemble of nitrogen-vacancy (NV) centers in a single-crystal diamond with an extended dynamic range for monitoring the fast changing magnetic-field. The NV-center spin resonance frequency is tracked using a closed-loop frequency locked technique with fast frequency hopping to achieve a 10 kHz measurement bandwidth, thus, allowing for the detection of fast changing magnetic signals up to 0.723 T/s.This technique exhibits an extended dynamic range subjected to the working bandwidth of the microwave source. This extended dynamic range can reach up to 4.3 mT, which is 86 times broader than the intrinsic dynamic range. The essential components for NV spin control and signal processing such as signal generation, microwave frequency control, data processing and readout are integrated in a board-level system. With this platform, we demonstrate broadband magnetometry with an optimized sensitivity of 4.2 nT-Hz-1/2. This magnetometry method has the potential to be implemented in a multichannel frequency locked vector magnetometer suitable for a wide range of practical applications such as magnetocardiography and high-precision current sensors., Comment: 18 pages, 9 figures

We previously designed a dual-axis piezoelectric MEMS mirror with a low crosstalk gimbal structure, which is utilized as the key device for further research for laser beam scanning. This paper mainly focuses on studying the Lissajous scanning resolution of this MEMS mirror with frequency ratio and phase modulation. For accurately evaluating the scanning resolution, the center angular resolution of Lissajous scanning is redefined by theoretical calculation and verified with experimental measurement. Meanwhile, the scanning nonlinearity of MEMS mirror is studied carefully. Finally, the MEMS mirror works at the state of pseudo-resonance, and the center angular resolution better than 0.16° (H) × 0.03° (V) is achieved at a scanning Field of view (FoV) of 35.0° (H) × 16.5° (V). Moreover, a feasible route of resolution adjustable Lissajous scanning is provided by optimization of frequency ratio and phase modulation, which is helpful for high definition and high frame rate (HDHF) laser scanning imaging with the dual-axis mirror.

The hydrogen bond formation with formic acid would affect the complementary pair of bases between uracil and adenine, but the binding modes and spectral properties of hydrogen bonds are still obscure. Density functional theory and time-dependent density functional theory were applied to investigate the intermolecular hydrogen bonds between uracil and formic acid. The reduced density gradient (RDG), bond lengths and vibration absorption frequencies revealed that the most probable uracil-formic acid (U-FA) interaction mode formed in the position c of FA and the site 1 of U, that is, the mode 1c. The theoretical parameters in excited state complexes manifested that the variety of hydrogen bond configurations led to different degrees of strengthening or weakening of molecular interaction. In the implicit solvent (water), the formations of O-H∙∙∙O in the uracil-formic acid complexes were promoted obviously. These theoretical studies would positively affect the researches of life science and medicinal chemistry.

This paper presents a simple and reliable routine for batch fabrication of wear-resistant and conductive probe with a PtSi tip. The fabrication process is based on inductively coupled plasma (ICP) etching, metal evaporation, and annealing. Si tips with curvature radii less than 10 nm were produced with good wafer-level uniformity using isotropic etching and thermal oxygen sharpening. The surface roughness of the etched tip post was reduced by optimized isotropic etching. The dependence of the platinum silicide morphology on annealing conditions were also systematically investigated, and conductive and wear-resistant probes with PtSi tips of curvature radii less than 30 nm were batch fabricated and applied for scanning piezoelectric samples.

Pepper (Capsicum spp.) is one of the earliest domesticated crops, providing a unique pungent sensation when eaten. Through the construction of the first pepper variome, we describe the main groups that emerged during domestication and breeding of C. annuum, their relations and temporal succession, and the molecular events underlying the main transitions. The initial differentiation in fruit shape and pungency, increase in fruit weight, and transition from erect to pendent fruits, and the recent appearance of blocky, large, sweet fruits (bell peppers), were accompanied by strong selection/fixation of key alleles and introgressions in two large genomic regions. Furthermore, we describe the identification of Up, a key domestication gene controlling erect vs pendent fruit orientation, encoding a BIG GRAIN protein involved in auxin transport, and Flip1 associated with capsaicinoid content, encoding a protein involved in phospholipid flipping. The function of Up was confirmed by virus-induced gene silencing. These findings constitute a cornerstone for understanding the domestication and differentiation of a key horticultural crop.

Slow desalination kinetics and poor durability of the electrodes are two key limitations of electrochemical deionization (EDI) that are considered to be the next generation of capacitive desalination (CDI). Herein, we report the design of a high-efficiency chloride removal electrode material for accelerating the desalination kinetics and concurrently improving the durability of EDI, which is based on coating NiMn-Cl layered double hydroxides (LDHs) on the surface of electrospun carbon nanofibers (CNFs@LDHs). The salient features of the as-developed CNFs@LDHs are that applying layer-structured LDHs with abundant redox-active sites to accelerate the pseudo-capacitive ion storage via fast ion intercalation/deintercalation, and leveraging the rigid CNF backbone to strengthen its durability by preventing the potential aggregation of LDHs. As expected, the CNFs@LDH based EDI system displays an ultrafast desalination rate of 0.51 mg g-1 s-1 and outstanding long-term stability (only 10.66 % desalination capacity reduction after 35 cycles), which is achieved without sacrificing its excellent desalination capacity (72.04 mg g-1). This work could be inspirational for the future design of ultrafast yet durable EDI approaching industrial desalination applications.

Corrole and oxaporphyrin have been successfully synthesized and applied in many research fields such as organic photoelectronics and sensors with the unique photophysical and chemical properties. However, the low synthesis yields of oxacorrole drive researchers turning their attention to theoretical studies for more reasonable molecular structure as the appeal of energy conservation and green chemistry. Corroles, oxacorroles (OC) and dioxacorroles (DOC), a total of 14 molecules, are calculated to systematically explore their structures, intramolecular hydrogen bonds, molecular aromatic and absorption spectral properties influenced by the inner O atoms positions with density functional theory (DFT) and time-dependent density functional theory (TDDFT). The smaller NICS(1)

Fertility restoration of cytoplasmic male sterility (CMS) in Capsicum annuum is controlled by multiple alleles of Restorer-of-fertility (Rf) genes. The isolation of additional Rf genes should therefore enrich the knowledge of CMS/Rf systems and accelerate their exploitation in hybrid seed production. In this study, the fertility restorer gene CaRfm of ‘0601 M’, a non-pungent bell pepper, was genetically mapped to a 1.2-cM region flanked by KASP markers S761 and S183. CaRfm was then physically mapped to a 128.96-Kb interval predicted from 24 recombinants with two co-segregated markers, S423 and S424. CaPPR6 encoding a pentatricopeptide repeat (PPR) protein was suggested as the most likely candidate gene for the CaRfm locus on the basis of sequence alignment as well as genotyping of tightly linked markers. In addition, molecular markers S1597 and S1609, which are immediately adjacent to CaRfm at 15.7 and 57.8-Kb respectively, were developed and applied to marker-assisted selection. The results provided friendly markers for breeding pepper restorer lines and laid the foundation for elucidating the male fertility restoration mechanism. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-021-01109-9.

Heparan sulfate (HS) is a sulfated polysaccharide presenting on the animal cell surface and in the extracellular matrix that plays a critical role in a range of different biological processes. The receptor for advanced glycation end-products (RAGE), as a major receptor linked to numerous diseases, can interact with HS to activate signaling of RAGE ligands in the disease process. We herein report a concise chemoenzymatic approach for the synthesis of HS-mimetic glycopolymers as RAGE antagonists. Glycopolymer with GlcA units was chemically synthesized and subsequently modified by enzymatic elongation and chemical sulfation. Six HS-mimetic glycopolymers with pendant sulfated di-, tri-, or tetrasaccharides were successfully prepared for evaluation of their interaction with RAGE. Upon evaluation, the HS-mimetic glycopolymer decorated with overall sulfated trisaccharides was determined to have the highest binding affinity with RAGE. These findings would promote the further exploration of HS-RAGE interactions and the development of RAGE antagonists.

This work reports a batch fabrication process for silicon nanometer tip based on isotropic inductively coupled plasma (ICP) etching technology. The silicon tips with nanometer apex and small surface roughness are produced at wafer-level with good etching homogeneity and repeatability. An ICP etching routine is developed to make silicon tips with apex radius less than 5 nm, aspect ratio greater than 5 at a tip height of 200 nm, and tip height more than 10 &mu, m, and high fabrication yield is achieved by mask compensation and precisely controlling lateral etch depth, which is significant for large-scale manufacturing.

Eukaryotic filamentous yellow-green algae from the Tribonema genus are considered to be excellent candidates for biofuels and value-added products, owing to their ability to grow under autotrophic, mixotrophic, and heterotrophic conditions and synthesize large amounts of fatty acids, especially unsaturated fatty acids. To elucidate the molecular mechanism of fatty acids and/or establish the organism as a model strain, the development of genetic methods is important. Towards this goal, here, we constructed a genetic transformation method to introduce exogenous genes for the first time into the eukaryotic filamentous alga Tribonema minus via particle bombardment. In this study, we constructed pSimple-tub-eGFP and pEASY-tub-nptⅡ plasmids in which the green fluorescence protein (eGFP) gene and the neomycin phosphotransferase Ⅱ-encoding G418-resistant gene (nptⅡ) were flanked by the T. minus-derived tubulin gene (tub) promoter and terminator, respectively. The two plasmids were introduced into T. minus cells through particle-gun bombardment under various test conditions. By combining agar and liquid selecting methods to exclude the pseudotransformants under long-term antibiotic treatment, plasmids pSimple-tub-eGFP and pEASY-tub- nptⅡ were successfully transformed into the genome of T. minus, which was verified using green fluorescence detection and the polymerase chain reaction, respectively. These results suggest new possibilities for efficient genetic engineering of T. minus for future genetic improvement.

Eukaryotic filamentous yellow-green algae from the Tribonema genus are considered to be excellent candidates for biofuels and value-added products, owing to their ability to grow under autotrophic, mixotrophic, and heterotrophic conditions and synthesize large amounts of fatty acids, especially unsaturated fatty acids. To elucidate the molecular mechanism of fatty acids and/or establish the organism as a model strain, the development of genetic methods is important. Towards this goal, here, we constructed a genetic transformation method to introduce exogenous genes for the first time into the eukaryotic filamentous alga Tribonema minus via particle bombardment. In this study, we constructed pSimple-tub-eGFP and pEASY-tub-nptⅡ plasmids in which the green fluorescence protein (eGFP) gene and the neomycin phosphotransferase Ⅱ-encoding G418-resistant gene (nptⅡ) were flanked by the T. minus-derived tubulin gene (tub) promoter and terminator, respectively. The two plasmids were introduced into T. minus cells through particle-gun bombardment under various test conditions. By combining agar and liquid selecting methods to exclude the pseudotransformants under long-term antibiotic treatment, plasmids pSimple-tub-eGFP and pEASY-tub- nptⅡ were successfully transformed into the genome of T. minus, which was verified using green fluorescence detection and the polymerase chain reaction, respectively. These results suggest new possibilities for efficient genetic engineering of T. minus for future genetic improvement.

Additional file 1: Table S1. Sequences of the SSR, InDel, and KASPar markers used in this study to map AnRGO5 on chromosome 5. Table S2. Sequences of primers used to perform RT-qPCR experiments. Table S3. Sequences of primers used to clone candidate genes.

A sequence-to-sequence (seq2seq) learning with neural networks empirically shows to be an effective framework for grammatical error correction (GEC), which takes a sentence with errors as input and outputs the corrected one. However, the performance of GEC models with the seq2seq framework heavily relies on the size and quality of the corpus on hand. We propose a method inspired by adversarial training to generate more meaningful and valuable training examples by continually identifying the weak spots of a model, and to enhance the model by gradually adding the generated adversarial examples to the training set. Extensive experimental results show that such adversarial training can improve both the generalization and robustness of GEC models.

The photochemical reaction process of 4-methyl-7-hydroxycoumarin (7H4MC) was studied by UV-Vis spectrometer and fluorescence spectroscopy. The results have shown that the characteristic peak of the UV-Vis spectra at 323 nm of 7H4MC undergoes a significant decrease in absorbance and a slight blue shift when was illuminated; the absorbance of the absorption peak at 279 nm rises, and there is a tendency for new peaks to appear. May be, there are structural changes or new materials generation. The addition of imidazole, VC, 3-indole methanol, GSH and other substances also have different effects on the photochemical reaction of 7H4MC. Among them, only imidazole has a certain inhibitory effect on the process of its photochemical reaction.

Background Colletotrichum species are the causal agents of anthracnose, a major disease affecting the yield and quality of pepper (Capsicum spp.). Colletotrichum scovillei is widespread in China, has strong pathogenicity and drug resistance, and causes anthracnose disease in pepper fruits that severely reduces production. Previously, an anti-anthracnose locus AnRGO5 was mapped to the P5 chromosome on the basis of analyses of fruit at the green mature stage. The aim of this study was to narrow down the interval of this locus and identify the gene responsible for conferring resistance. Results On the basis of results of re-sequencing of Capsicum chinense ‘PBC932’ and C. annuum ‘77013’, we developed Kompetitive allele-specific PCR (KASPar) markers and insertion–deletion (InDel) markers linked to AnRGO5 at the green mature fruit stage and used them to construct a genetic linkage map (42 markers, 24.4 cM in length). Using data obtained in phenotypic and genotypic analyses of BC4S1, BC4S2, and BC4S3 populations, AnRGO5 was located between the markers P5in-2266-404 and P5in-2268-978 within a physical distance of 164 kb. This region contained five genes, including CA05g17730. CA05g17730 encodes ‘R1C-3-like’ putative late blight resistance protein homologs. The transcript level of CA05g17730 differed between ‘PBC932’ and ‘77013’. The structure of the CA05g17730 gene also differed between ‘PBC932’ and ‘77013’. Conclusions We narrowed down the QTL interval to a region containing five genes. These results will be useful for further research on the mechanisms of resistance to anthracnose, and for marker assisted selection for anthracnose-resistant capsicum lines.

The equation error method, output error method, and neural network method are used to model the aircraft longitudinal aerodynamic coefficients from simulated flight data. The equation error method and the output error method are based on postulated mathematical models of the longitudinal aerodynamic coefficients, and the least squared principle and the maximum likelihood principle are used to estimate the model parameters respectively. The neural network method uses the BP network output to fit the aerodynamic coefficients without assumptions of mathematical model of aerodynamic coefficients. Two sets of simulation tests were conducted, one set of data is used for model identification, and the other set is used for model validation.