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Background: Recruitment and retention of participants are important factors in empirical studies. Methods that increase recruitment and retention can reduce costs and burden on researchers related to the need for over-recruitment because of attrition. Rideshare services such as Uber and Lyft are a potential means for decreasing this burden., Objective: This study aimed to understand the role rideshare utilization plays in participant recruitment and retention in research trials., Methods: Data are presented for a study (N=42) in which rideshare services were utilized for participant transportation to and from study visits during a 2-session, in-laboratory research study., Results: Retention at visit 2 was greater than 95% (42/44) in the initial study. In a follow-up survey of the participants from the original trial, participants (N=32) reported that the rideshare service was an important reason they returned for all study visits. Participants reported whether they would prefer differing levels of additional monetary compensation or a ride from a rideshare service. When the additional compensation was less than US $15, participants reported a preference for the rideshare service., Conclusions: Rideshare services may represent a relatively low cost means for increasing study retention. Specifically, findings indicate that rideshare services may not be crucial for initial participant recruitment but for their retention in multi-visit studies., (©Eleanor Ladd Schneider Leavens, Elise Marie Stevens, Emma Irene Brett, Neil Molina, Thad Ryan Leffingwell, Theodore Lee Wagener. Originally published in the Journal of Medical Internet Research (http://www.jmir.org), 08.04.2019.)