13 results on '"Le, Annie"'
Search Results
2. Anti-Asian Bullying and Harassment: Symptoms of Racism in K-12 Schools during COVID-19. The Opportunity for Race-Conscious Policy and a More Equitable California. Research in Brief
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Education Trust-West, Le, Annie, Gutierrez, Rose Ann E., and Teranishi, Robert T.
- Abstract
The COVID-19 pandemic has posed many challenges for K-12 students. However, these challenges have not been experienced equally across student groups. There has been a significant increase in mainstream media coverage of anti-Asian racism, but very little attention has been given to Asian American youth, who are not immune from incidents of bullying and harassment in our K-12 schools. This brief discusses how Asian American and Pacific Islander (AAPI) students face unique challenges associated with bullying and harassment because of their racial and ethnic identity. We examine the historical context of bullying and harassment of Asian Americans and how that persists as anti-Asian racism today. Finally, we propose policy solutions to create a more positive learning environment and address racist attitudes towards this specific community. Notably, we propose that K-12 leaders disaggregate data by ethnic subgroup, collect more comprehensive data on school bullying, harassment, and victimization disaggregated by AAPI ethnic subgroups, and invest in culturally sustaining mental health resources and curriculum.
- Published
- 2021
3. Discoidin domain receptor 1 (DDR1) ablation promotes tissue fibrosis and hypoxia to induce aggressive basal-like breast cancers
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Takai, Ken, Drain, Allison P, Lawson, Devon A, Littlepage, Laurie E, Karpuj, Marcela, Kessenbrock, Kai, Le, Annie, Inoue, Kenichi, Weaver, Valerie M, and Werb, Zena
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Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Immunology ,Lung ,Cancer ,Women's Health ,Breast Cancer ,Animals ,Breast Neoplasms ,Cell Hypoxia ,Discoidin Domain Receptor 1 ,Disease-Free Survival ,Epithelial Cells ,Female ,Fibrosis ,Hypoxia-Inducible Factor 1 ,alpha Subunit ,Lung Neoplasms ,Mammary Neoplasms ,Experimental ,Mice ,DDR1 ,mammary development ,breast cancer ,necrosis/hypoxia ,basal-like phenotype ,Biological Sciences ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Developmental Biology ,Biological sciences ,Biomedical and clinical sciences ,Psychology - Abstract
The discoidin domain receptor 1 (DDR1) is overexpressed in breast carcinoma cells. Low DDR1 expression is associated with worse relapse-free survival, reflecting its controversial role in cancer progression. We detected DDR1 on luminal cells but not on myoepithelial cells of DDR1+/+ mice. We found that DDR1 loss compromises cell adhesion, consistent with data that older DDR1-/- mammary glands had more basal/myoepithelial cells. Basal cells isolated from older mice exerted higher traction forces than the luminal cells, in agreement with increased mammary branches observed in older DDR1-/- mice and higher branching by their isolated organoids. When we crossed DDR1-/- mice with MMTV-PyMT mice, the PyMT/DDR1-/- mammary tumors grew faster and had increased epithelial tension and matricellular fibrosis with a more basal phenotype and increased lung metastases. DDR1 deletion induced basal differentiation of CD90+CD24+ cancer cells, and the increase in basal cells correlated with tumor cell mitoses. K14+ basal cells, including K8+K14+ cells, were increased adjacent to necrotic fields. These data suggest that the absence of DDR1 provides a growth and adhesion advantage that favors the expansion of basal cells, potentiates fibrosis, and enhances necrosis/hypoxia and basal differentiation of transformed cells to increase their aggression and metastatic potential.
- Published
- 2018
4. From Particularities to Context: Refining Our Thinking on Illness Narratives
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Le, Annie, Miller, Kara, and McMullin, Juliet
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Health Services and Systems ,Philosophy and Religious Studies ,Health Sciences ,Clinical Research ,Cultural Competency ,Education ,Medical ,Empathy ,Health Equity ,Humans ,Literature ,Modern ,Moral Obligations ,Narration ,Patient Care ,Physician-Patient Relations ,Reference Standards ,Stereotyping ,Teaching ,Thinking - Abstract
This paper examines how illness narratives are used in medical education and their implications for clinicians' thinking and care of patients. Ideally, collecting and reading illness narratives can enhance clinicians' sensitivity and contextual thinking. And yet these narratives have become part of institutionalizing cultural competency requirements in ways that tend to favor standardization. Stereotyping and reductionistic thinking can result from these pedagogic approaches and obscure structural inequities. We end by asking how we might best teach and read illness narratives to fulfill the ethical obligations of listening and asking more informative clinical interview questions that can better meet the needs of patients and the community.
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- 2017
5. Targeting the cancer-associated fibroblasts as a treatment in triple-negative breast cancer
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Takai, Ken, Le, Annie, Weaver, Valerie M, and Werb, Zena
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Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Immunology ,Cancer ,Lung ,Breast Cancer ,Lung Cancer ,2.1 Biological and endogenous factors ,Aetiology ,Animals ,Antineoplastic Agents ,Cancer-Associated Fibroblasts ,Cell Line ,Tumor ,Cell Proliferation ,Collagen ,Dose-Response Relationship ,Drug ,Female ,Humans ,Lung Neoplasms ,Mice ,Inbred BALB C ,Molecular Targeted Therapy ,Pyridones ,Signal Transduction ,Time Factors ,Transforming Growth Factor beta ,Triple Negative Breast Neoplasms ,Tumor Burden ,Tumor Cells ,Cultured ,Tumor Microenvironment ,Xenograft Model Antitumor Assays ,pirfenidone ,triple-negative breast cancer ,fibrosis ,cancer-associated fibroblast ,transforming growth factor-beta ,transforming growth factor-β ,Oncology and carcinogenesis - Abstract
Increased collagen expression in tumors is associated with increased risk of metastasis, and triple-negative breast cancer (TNBC) has the highest propensity to develop distant metastases when there is evidence of central fibrosis. Transforming growth factor-β (TGF-β) ligands regulated by cancer-associated fibroblasts (CAFs) promote accumulation of fibrosis and cancer progression. In the present study, we have evaluated TNBC tumors with enhanced collagen to determine whether we can reduce metastasis by targeting the CAFs with Pirfenidone (PFD), an anti-fibrotic agent as well as a TGF-β antagonist. In patient-derived xenograft models, TNBC tumors exhibited accumulated collagen and activated TGF-β signaling, and developed lung metastasis. Next, primary CAFs were established from 4T1 TNBC homograft tumors, TNBC xenograft tumors and tumor specimens of breast cancer patients. CAFs promoted primary tumor growth with more fibrosis and TGF-β activation and lung metastasis in 4T1 mouse model. We then examined the effects of PFD in vitro and in vivo. We found that PFD had inhibitory effects on cell viability and collagen production of CAFs in 2D culture. Furthermore, CAFs enhanced tumor growth and PFD inhibited the tumor growth induced by CAFs by causing apoptosis in the 3D co-culture assay of 4T1 tumor cells and CAFs. In vivo, PFD alone inhibited tumor fibrosis and TGF-β signaling but did not inhibit tumor growth and lung metastasis. However, PFD inhibited tumor growth and lung metastasis synergistically in combination with doxorubicin. Thus, PFD has great potential for a novel clinically applicable TNBC therapy that targets tumor-stromal interaction.
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- 2016
6. Comparative Lipidomics of Oral Commensal and Opportunistic Bacteria.
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Wood, Paul L., Le, Annie, and Palazzolo, Dominic L.
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LIPIDOMICS ,STREPTOCOCCUS sanguis ,GRAM-positive bacteria ,GRAM-negative bacteria ,STREPTOCOCCUS mutans ,CANDIDA albicans ,DIROFILARIA immitis - Abstract
The oral cavity contains a vast array of microbes that contribute to the balance between oral health and disease. In addition, oral bacteria can gain access to the circulation and contribute to other diseases and chronic conditions. There are a limited number of publications available regarding the comparative lipidomics of oral bacteria and fungi involved in the construction of oral biofilms, hence our decision to study the lipidomics of representative oral bacteria and a fungus. We performed high-resolution mass spectrometric analyses (<2.0 ppm mass error) of the lipidomes from five Gram-positive commensal bacteria: Streptococcus oralis, Streptococcus intermedius, Streptococcus mitis, Streptococcus sanguinis, and Streptococcus gordonii; five Gram-positive opportunistic bacteria: Streptococcus mutans, Staphylococcus epidermis, Streptococcus acidominimus, Actinomyces viscosus, and Nanosynbacter lyticus; seven Gram-negative opportunistic bacteria: Porphyromonas gingivalis. Prevotella brevis, Proteus vulgaris, Fusobacterium nucleatum, Veillonella parvula, Treponema denticola, and Alkermansia muciniphila; and one fungus: Candida albicans. Our mass spectrometric analytical platform allowed for a detailed evaluation of the many structural modifications made by microbes for the three major lipid scaffolds: glycerol, sphingosine and fatty acyls of hydroxy fatty acids (FAHFAs). [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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7. Beyond Usual Suspects: The Racial Paradox of Asian American and Pacific Islander Gang Affiliated Youth
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Le, Annie
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Education - Abstract
Youth gangs have been described as one of the most important urban youth issues in society today. Given the permeance of street gangs in economically marginalized communities inhabited by predominantly people of color, youth gang formation, membership, and criminalization carry racial connotations. Compared to other racial groups, however, far less is known on Asian American and Pacific Islander (API) youth gangs, despite long standing gang presence in this community. The absence of APIs in this topic of inquiry implies their experiences can be understood in the absence of racial context which contributes to their problematic representation as the “model minority.” Drawing on Asian critical race theory, this qualitative study utilizes a critical phenomenological approach to explore how 30 gang affiliated API youth understand their racial identity and experiences through in-depth phenomenological interviews. Building on an ethnographic pilot study of Asian American gang affiliated youth in a schooling context, findings from this study suggest the unique origin and evolution of API youth involvement in gangs. Specifically, there is a generational distinction when it came to gang membership. Findings also demonstrate API youth rely on various strategies of racial identity construction through their gang affiliation. For example, they embrace racialized stereotypes and ascribe racial/ethnic meaning to cultural practices to make meaning of their identity. Finally, findings reveal API youth adopt the “schoolboy gangster” paradox to navigate the tensions of their racial and gang identity. This experience produces a double life where they play the role of being a devoted son at home and in schools to hide their identity as gang members. This study provides empirical contribution to a group overlooked in gang subculture and bridges the theoretical gap between youth gangs and racial categorization. Additionally, this study offers implications for policy and practice in the field of urban education committed to racial equity and social justice.
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- 2021
8. The Language and Culture of Costume Design
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Le, Annie
- Subjects
Theater ,Costume Design - Abstract
When we think of language and culture, we often think of our ethnical background, along with the influences from our society, history, media and the people around us. However, through my years here at UCSD, I have learned that there is a different form of language and culture in Costume Design. Culture impacts how we think and language helps us communicate; both play a major part in us understanding one another. For each show that I worked on, I’ve had to learn a new language to speak to directors, actors, and costume technicians. The language that I am speaking of is not English, French, or Vietnamese but rather it pertains to the choice of words I use to communicate my design. For culture, as a costume designer, we are always balancing between pop culture and historical culture. Kyle Donnelly’s adaptation of Taming of the Shrew(d), the play was set in a post-apocalyptic world where a group of women put of plays in exchange for food and water. Donnelly was highly influenced by her environment when she pitched this idea – requests in the department, politics, and the movie, Mad Max: Fury Road, which was very popular at the time. For the development of my design, I researched pop culture’s different ideas of post-apocalyptic worlds as well as the history of American stereotypes in women’s clothing and how it relates to their job (i.e. the waitress, the businesswoman, the stay-at-home mom). In my pop culture research (film & television, modern art, comics, etc.), depictions of post-apocalypse worlds are often characterized by distressed clothing, dirt, make-shift weapons and recycling, which I used in my overall design. For each individual character’s look, historical culture was a major influence – the cut of a business suit jacket, the recognizable color and shape of a waitress uniform. Knowing both pop and historical culture helped me develop the language I needed to clearly convey my ideas.To gain both the director’s and actors’ confidence, I had to educate myself and used terms they would understand by referencing things they are exposed to in their daily lives – film, art, music. I also focused on character development – the backstory, the mental and physical state, education and more. For example, Hannah Finn’s character story was that before the apocalypse, she was a high school dropout working as a waitress at a diner in a small town. She was poor, uneducated but funny and loves to make jokes. She was at work when the apocalypse took place, which explains why she’s still in her work uniform. Her character also wears a chest armor made from cans and uses a large ladle that has nails through it as a weapon (to mimic a mace), which was inspired by things she would have found in the diner kitchen. Both the director and the actor loved the visual and emotional story created for the character and understood the concept that I was going for. When presenting to the costume shop, I introduced the general post-apocalyptic theme, however, instead of talking about each character’s backstory, we spoke about construction, fit and function of each outfit. The language use when speaking to the shop is much more technical. I had to learn clothing and construction terminology – such as raglan sleeve, a gather, a blind stitch, etc. – to properly articulate my design. Using Finn’s costume as an example again, with the technicians, we talked about the history of the style of the uniform and how it should fit, where wear and tear should be, how distressed it should look and how the can apron should be constructed to look like it was home-made. Costume vocabulary and details are very important when it comes to the construction. I was very specific when providing Finn’s actions in the play and the technicians offered solutions to some clothing restrictions she was experiencing, such as adding a gusset to the underarm area of the sleeve to give her more room to stretch her arms up. Without knowing the proper language, we would have had a harder time understanding the needs of the show. Marco Barricelli’s production of The Green Cockatoo was an entirely different language and culture from Shrew(d). Barricelli and the actors has little to no knowledge of the French period and culture for which this play was written. To make is easier for them to understand, I provided historical reference images, such as paintings and French fashion plates, as well as research from pop culture (the movie, Marie Antoinette) to help familiarize the look. I also focused on colors to differentiate social class, and researched the French body (i.e. how they walk, stand, sit, hold an accessory, etc.) to help the actors fully embrace the period and help create a visual realistic piece for the director. All parties would want to know why I made the choices I made and having that knowledge puts confidence in my answers and modernizing some costume pieces gave comfort to everyone, physically and mentally. For this production, I had seven corsets, two dresses and two men’s coats built. The shop’s focus was on how clothing was accurately constructed during this time and learning about all the understructure need to create the silhouette of the period. Together, we studied period clothing patterns, use of fabric and embellishments. My design was a mix of historical reference and self-imagination and the language of period clothing helped both the shop and I to fully realize each piece. For Claire Roberson’s character, Severine, we exaggerated the width of her hips by using a larger bum roll to emphasize her status and wealth. From the cut of her dress to the intricate beaded lace, along with a wig and white face makeup, all these technical changes helped elevate her character further, while also being semi-period appropriate. A famous quote from Judith Dolan, “We don’t copy the period, we use the period.” The language and culture of contemporary shows, such as Go. Please. Go, and (w)holeness, in my opinion, are more intricate and challenging. We deal with choosing our own outfits, what accessories to wear and how to do our hair daily, thus it is often difficult to separate fashion from costumes. This proven to be so while working on my most recent production, Revolt. She Said. Revolt Again. The director, Sean Graney, wanted the costumes to be motivated by what the actors wear in their daily life instead of what clothes the character they were playing would wear, thus blurring the line of fashion and costume. I did a background research on all the actors to learn their style while also trying to appeal to Graney’s taste in clothes. It was difficult navigating eight different types of styles, all influenced by their different cultures and the language used to speak to men verse speaking to women about clothes also came in to play. However, another important factor was missing (which led to some miscommunication) and that was the generational difference between the director and the actors. Having allowed the actors the freedom to choose their own costumes, they introduced fashion that they are currently attracted to, one being the crop top. I learned that Graney dislikes the crop top because it reminds him of the fashion he grew up with in the 90s, which he thought was tacky, therefore, in his mind, all crop tops are tacky. Neither could fully understand why the other liked or disliked the crop top and it was because of this fashion cultural difference. I learned when speaking with the actors, I talked about how their individual style adds to the show, and with the director, I spoke of the group as an ensemble and how it visually relates to the rest of the production. On the other hand, the shop technicians did not care for current or past fashion, rather we focused on detailing and visual effect. The colors of this show were red, brown and white, and the technicians helped me incorporate all three colors into each outfit without making them look too absurd or abnormal. Such an example would be in Mary-Rose Branick’s costume. Branick had a red polo shirt with brown corduroy overalls. She was missing white in her outfit and so we took out her brown zipper and replaced it with a white zipper. Having everyone in a controlled color pallet and changing minor details helped to push the clothes further to looking like costumes than ordinary street clothes. The language of costume construction was key in helping to make such changes. Every show that I’ve worked on has provided me with a different experience and new challenges. The languages and cultures I’ve learned has assisted me in helping the director visualize the production, understand how it relates to the actor and the character they are playing and how to properly communicate with the team who builds these items. The language we speak and cultures we learn is used to appeal to the visual, the emotional and the technical. Therefore, costume designers must be multi-cultural and multi-lingual to be successful.
- Published
- 2018
9. The Difficult-to-Control Asthmatic: A Systematic Approach
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Le Annie V and Simon Ronald A
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract With the judicious use of inhaled corticosteroids, β2 agonists, and leukotriene modifiers, most patients with asthma are easily controlled and managed. However, approximately 5% of asthmatics do not respond to standard therapy and are classified as "difficult to control." 1 Typically, these are patients who complain of symptoms interfering with daily living despite long-term treatment with inhaled corticosteroids in doses up to 2,000 μg daily. Many factors can contribute to poor response to conventional therapy, and especially for these patients, a systematic approach is needed to identify the underlying causes. First, the diagnosis of asthma and adherence to the medication regimen should be confirmed. Next, potential persisting exacerbating triggers need to be identified and addressed. Concomitant disorders should be discovered and treated. Lastly, the impact and implications of socioeconomic and psychological factors on disease control can be significant and should be acknowledged and discussed with the individual patient. Less conventional and novel strategies for treating corticosteroid-resistant asthma do exist. However, their use is based on small studies that do not meet evidence-based criteria; therefore, it is essential to sort through and address the above issues before reverting to other therapy.
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- 2006
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10. MAPK phosphatase-1 facilitates the loss of oxidative myofibers associated with obesity in mice
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Roth, Rachel J., Le, Annie M., Zhang, Lei, Kahn, Mario, Samuel, Varman T., Shulman, Gerald I., and Bennett, Anton M.
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Obesity ,Phosphatases ,Health care industry - Abstract
Oxidative myofibers, also known as slow-twitch myofibers, help maintain the metabolic health of mammals, and it has been proposed that decreased numbers correlate with increased risk of obesity. The transcriptional coactivator PPARγ coactivator 1α (PGC-1α) plays a central role in maintaining levels of oxidative myofibers in skeletal muscle. Indeed, loss of PGC-1α expression has been linked to a reduction in the proportion of oxidative myofibers in the skeletal muscle of obese mice. MAPK phosphatase-1 (MKP-1) is encoded by mkp-1, a stress-responsive immediate-early gene that dephosphorylates MAPKs in the nucleus. Previously we showed that mice deficient in MKP-1 have enhanced energy expenditure and are resistant to diet-induced obesity. Here we show in mice that excess dietary fat induced MKP-1 overexpression in skeletal muscle, and that this resulted in reduced p38 MAPK-mediated phosphorylation of PGC-1α on sites that promoted its stability. Consistent with this, MKP-1-deficient mice expressed higher levels of PGC-1α in skeletal muscle than did wild-type mice and were refractory to the loss of oxidative myofibers when fed a high-fat diet. Collectively, these data demonstrate an essential role for MKP-1 as a regulator of the myofiber composition of skeletal muscle and suggest a potential role for MKP-1 in metabolic syndrome., Introduction The MAPK pathway plays a critical role in physiological and pathophysiological processes. The growth factor-responsive Erks (Erk1 and Erk2) and the stress-responsive MAPKs (p38 MAPK and JNK) are established [...]
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- 2009
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11. (Re)conceptualizing Protests: Activism, Resistance, and AANAPISIs
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Gutierrez, Rose Ann E., primary and Le, Annie, additional
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- 2018
- Full Text
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12. Overexpression of SMURF1 inhibits BMP signaling in chicken articular chondrocytes and murine C3H10T1/2 multipotent mesenchymal cells
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Le, Annie and Le, Annie
- Abstract
Advised by Michael Zuscik, Ph.D., Department of Orthopaedics, University of Rochester Medical Center.
13. Overexpression of SMURF1 inhibits BMP signaling in chicken articular chondrocytes and murine C3H10T1/2 multipotent mesenchymal cells
- Author
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Le, Annie and Le, Annie
- Abstract
Advised by Michael Zuscik, Ph.D., Department of Orthopaedics, University of Rochester Medical Center.
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