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Brassicas are one of the most agronomically eminent oilseeds that are employed as a variety of oilseed, vegetable, and fodder crops. The experiment was conducted with thirty-four genotypes/varieties seeds of Indian mustard [Brassica juncea Czern & Coss (L.)] for oil content, Iodine value, oil stability index and fatty acid composition during 2018-2019. The experiment was laid out in completely randomized design with three replications. The range of variability of contents of oil, palmitate, stearate, oleate, linoleate, linolenate, ecosenate, doecosenate, iodine value and oil stability index varied from 33.52 to 42.15%, 1.53 to 4.98%, 0.16 to 2.71%, 5.06 to 17.78%, 17.88 to 32.15%, 11.82 to 19.85%, 5.44 to 11.89%, 28.82 to 47.66%, 114.43 to 131.71 and 1.08 to 1.99, respectively. The Brassica juncea genotype-KMR-15-6 followed by genotype- KMR-17-6 had the higher oil content, oleic acid content and low value of erucic acid which indicates that seed oil this B. juncea species genotype is possibly suitable for both human consumption and industrial purposes.