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15 results on '"Komatsu, Yumiko"'

4. Eel Serum Synergistically Enhances Growth of Fish Cell Line GAKS

Author

TAKUMI Shota, KOMATSU Yumiko, DOMEN Yasuhito, SUMIZAWA Tomoyuki, SHIOZAKI Kazuhiro, ISHIKAWA Manabu, KOMATSU Masaharu, HAYASHI Seiichi, and YAMADA Shoji

Subjects
cell culture, eel serum, fish cell line, GAKS
Abstract

Generation of cell lines derived from fish is getting to increase. Generally, mammalian cell line culture needs appropriate media with approximately 5 to 10 % fetal calf serum. However, the optimal culture conditions for fish cell lines have not been established so far. We investigated the effect of exposure to inactivated fish serum on cell culture of goldfish scale cell line GAKS. The 5 % sera from gibel, carp, tilapia, and eel were toxic to GAKS cells. In addition, these fish serum were toxic to not only the GAKS, but also the mammalian cell lines HEK293 and SH-SY5Y. GAKS cells did not proliferate under the culture condition with 5% fish serum. Although it is known that fish serum contains thiaminase, the effect of supplemental addition of vitamin B1 was negligible for the proliferation of GAKS cells. Lower concentrations of fish sera, especially less than 5 % of eel serum stimulated the cell survival of GAKS compared with 5 % of each fish serum. The culture medium containing around 1 % eel serum coupled with 1% FCS was comparable to 5 % FCS conditions for proliferation of GAKS cells. Furthermore, the passage culture was successful under these culture conditions.

Published
2021

8. Safety and tolerability of diazoxide in Japanese patients with hyperinsulinemic hypoglycemia

Author

Komatsu, Yumiko, 1000070552420, Nakamura, Akinobu, Takihata, Masahiro, Inoue, Yuichiro, Yahagi, Satoko, Tajima, Kazuki, Tsuchiya, Hirohisa, Takano, Tatsuro, Yamakawa, Tadashi, Yoshida, Masahiro, 1000030360902, Miyoshi, Hideaki, Terauchi, Yasuo, Komatsu, Yumiko, 1000070552420, Nakamura, Akinobu, Takihata, Masahiro, Inoue, Yuichiro, Yahagi, Satoko, Tajima, Kazuki, Tsuchiya, Hirohisa, Takano, Tatsuro, Yamakawa, Tadashi, Yoshida, Masahiro, 1000030360902, Miyoshi, Hideaki, and Terauchi, Yasuo

Abstract

Diazoxide is a non-diuretic benzothiadiazine derivative, one of a group of substances introduced into clinical practice in the 1950s for the treatment of hypertension. Fajans reported the use of diazoxide for the treatment of insulinoma in 1979. Although patients with hyperinsulinemic hypoglycemia worldwide have been treated with diazoxide for more than 30 years, there are no recent reports about the adverse effects of this drug in Asian patients, including Japanese patients. Herein, we report the results of our retrospective clinical record review of 6 Japanese patients (3 females and 3 males, ranging in age from 58 to 91 years) with hyperinsulinemic hypoglycemia and inoperable insulinoma treated with diazoxide. Diazoxide improved control of hypoglycemic symptoms and maintained normoglycemia in 5 of the 6 patients, and was ineffective in one patient. Surprisingly, although all 6 patients received diazoxide according to the treatment strategy recommended in Western patients, 5 of the 6 patients developed edema and two developed congestive heart failure. Thus, when starting treatment with diazoxide in Japanese patients, the symptoms and signs of fluid retention should be evaluated carefully. Also, appropriate protocols for treatment with diazoxide should be evaluated by means of clinical trials in Japanese patients with hyperinsulinemic hypoglycemia.

Published
2016

10. Safety and tolerability of diazoxide in Japanese patients with hyperinsulinemic hypoglycemia

Author

Komatsu, Yumiko, primary, Nakamura, Akinobu, additional, Takihata, Masahiro, additional, Inoue, Yuichiro, additional, Yahagi, Satoko, additional, Tajima, Kazuki, additional, Tsuchiya, Hirohisa, additional, Takano, Tatsuro, additional, Yamakawa, Tadashi, additional, Yoshida, Masahiro, additional, Miyoshi, Hideaki, additional, and Terauchi, Yasuo, additional

Published
2016
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