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Here, we report a case of hepatocellular carcinoma detected on computed tomography and treated with laparoscopic anatomical liver resection in a 69-year-old woman who was being followed-up for hepatitis C. Intraoperative liver segmentation is necessary to accomplish laparoscopic anatomical liver resection. Therefore, the day before surgery, hepatic artery embolization was performed with an indocyanine green-Lipiodol Ⓡ mixture and Gelpart Ⓡ containing indocyanine green to mark the region for hepatectomy. The next day, surgeons visually confirmed the resection segments on indocyanine green fluorescence imaging and performed laparoscopic anatomical liver resection. No major complications resulted from this method. In conclusion, hepatic artery embolization with an indocyanine green-Lipiodol Ⓡ mixture is effective and safe for liver segment identification during laparoscopic anatomical liver resection., Competing Interests: The authors declare that they have no conflicts of interest to report. This case report has been presented at CIRSE2019., (© 2020 Japanese Society of Interventional Radiology.)