39 results on '"Jun-Sheng Zhang"'
Search Results
2. Indole Diterpenes from Mangrove Sediment-Derived Fungus Penicillium sp. UJNMF0740 Protect PC12 Cells against 6-OHDA-Induced Neurotoxicity via Regulating the PI3K/Akt Pathway
- Author
-
Xin-Xin Wang, Ze-Long Chen, Jun-Sheng Zhang, Hai-Shan Liu, Ruo-Ping Ma, Xin-Ping Liu, Ming-Yue Li, Di Ge, Jie Bao, and Hua Zhang
- Subjects
indole diterpenes ,Penicillium sp. ,neuroprotection ,PI3K/Akt pathway ,Biology (General) ,QH301-705.5 - Abstract
In our chemical investigation into Penicillium sp. UJNMF0740 derived from mangrove sediment, fourteen indole diterpene analogs, including four new ones, are purified by multiple chromatographic separation methods, with their structures being elucidated by the analyses of NMR, HR-ESIMS, and ECD data. The antibacterial and neuroprotective effects of these isolates were examined, and only compounds 6 and 9 exhibited weak antibacterial activity, while compounds 5, 8, and 10 showed protective effects against the injury of PC12 cells induced by 6-hydroxydopamine (6-OHDA). Additionally, compound 5 could suppress the apoptosis and production of reactive oxygen species (ROS) in 6-OHDA-stimulated PC12 cells as well as trigger the phosphorylation of PI3K and Akt. Taken together, our work enriches the structural diversity of indole diterpenes and hints that compounds of this skeleton can repress the 6-OHDA-induced apoptosis of PC12 cells via regulating the PI3K/Akt signaling pathway, which provides evidence for the future utilization of this fascinating class of molecules as potential neuroprotective agents.
- Published
- 2023
- Full Text
- View/download PDF
3. Sequencing and analysis of the complete mitochondrial genome of Micromys erythrotis from China and its phylogenetic analysis
- Author
-
He Cai, Qing-Qing Wang, Xin-Xu Zhao, Qian-Qian Yao, Ning Wu, Jun-Sheng Zhang, and Zhu Liu
- Subjects
control region ,mitogenome ,phylogenetic trees ,micromys erythrotis ,Genetics ,QH426-470 - Abstract
The complete mitogenome sequence of Micromys erythrotis was determined using long PCR. The genome was 16,238 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (33.7%), C (24.8%), T (29.1%) and G (12.4%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of Micromys erythrotis in Muridae at the molecular level. The mitochondrial genome would be a significant supplement for the Micromys erythrotis genetic background.
- Published
- 2021
- Full Text
- View/download PDF
4. Sequencing and analysis of the complete mitochondrial genome of Ochotona hyperborea from China and its phylogenetic analysis
- Author
-
Xin-Xu Zhao, Zhong-Wan Piao, Qiao-Jiang Yang, Qi Zhang, Qian-Qian Yao, Jun-Sheng Zhang, and Zhu Liu
- Subjects
control region ,mitogenome ,phylogenetic trees ,ochotona hyperborea ,Genetics ,QH426-470 - Abstract
The complete mitogenome sequence of Ochotona hyperborea was determined using long PCR. The genome was 17,063 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall base composition of the heavy strand is A (31.1%), C (28.7%), T (26.3%), and G (13.9%). The base compositions present clearly the A–T skew, which is most obvious in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of Ochotona hyperborea in Ochotonidae at the molecular level. The mitochondrial genome would be a significant supplement for the Ochotona hyperborea genetic background. The eight Ochotona species formed a monophyletic group with the high bootstrap value (100%) in all examinations.
- Published
- 2021
- Full Text
- View/download PDF
5. Identification of Small-Molecule Bioactive Constituents from the Leaves of Vaccinium bracteatum Confirms It as a Potential Functional Food with Health Benefits
- Author
-
Yin-Yin Wang, Jun-Sheng Zhang, Xin-Xin Wang, Lin-Lin Tian, Yu-Peng Li, Chao Wang, Ren-Fen Ma, Yi-Ke Yin, Jie Bao, and Hua Zhang
- Subjects
Vaccinium bracteatum ,antioxidant activity ,free radical scavenging activity ,neuroprotective activity ,iridoid ,lignan ,Chemical technology ,TP1-1185 - Abstract
The health benefits of Vaccinium bracteatum are well recorded in ancient Chinese medical books and were also demonstrated by modern researches. However, the relationship between its beneficial functions and specific chemical constituents has not been fully characterized. This study investigated the bioactive small-molecule constituents in the leaves of V. bracteatum, which afforded 32 compounds including ten new ones (1–9) and ten pairs of enantiomers (9–18). Their structures with absolute configurations were elucidated by spectroscopic methods, especially nuclear magnetic resonance (NMR) and electronic circular dichroism (ECD) analyses, with 1–4 bearing a novel revolving-door shaped scaffold. While half-compounds exhibited decent antioxidant activity by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, all except 19 and 20 exerted significant capturing activity against diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radicals. In addition, the new iridoids 1, 5, 6, and 7 exerted apparent neuroprotective activity toward PC12 cells, with 1 being comparable to the positive control, and selective compounds also displayed anti-diabetic and anti-inflammatory properties by inhibiting α-glucosidase and NO production, respectively. The current work revealed that the bioactive small-molecule constituents could be closely related to the functional food property of the title species.
- Published
- 2023
- Full Text
- View/download PDF
6. Peritoneal Metastasis After Treated With Abemaciclib Plus Fulvestrant for Metastatic Invasive Lobular Breast Cancer: A Case Report and Review of the Literature
- Author
-
Hong-Fei Gao, Jun-Sheng Zhang, Qiang-Zu Zhang, Teng Zhu, Ci-Qiu Yang, Liu-Lu Zhang, Mei Yang, Fei Ji, Jie-Qing Li, Min-Yi Cheng, Gang Niu, and Kun Wang
- Subjects
breast ,lobular carcinoma ,neoplasm metastasis ,peritoneum ,whole exome sequencing ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Peritoneal metastases from invasive lobular carcinoma (ILC) of breast are uncommon and usually related to poor prognosis due to difficulty of detection in clinical practice and drug resistance. Therefore, recognizing the entities of peritoneal metastases of ILC and the potential mechanism of drug resistance is of great significance for early detection and providing accurate management. We herein report a case of a 60-year-old female who presented with nausea and vomiting as the first manifestation after treated with abemaciclib (a CDK4/6 inhibitor) plus fulvestrant for 23 months due to bone metastasis of ILC. Exploratory laparotomy found multiple nodules in the peritoneum and omentum, and immunohistochemistry confirmed that the peritoneal metastatic lesions were consistent with ILC. Palliative therapy was initiated, but the patient died two months later due to disease progression with malignant ascites. Whole exome sequencing (WES) was used to detect the tumor samples and showed the peritoneal metastatic lesions had acquired ESR1 and PI3KCA mutations, potentially explaining the mechanism of endocrine therapy resistance. We argue that early diagnosis of peritoneal metastasis from breast cancer is crucial for prompt and adequate treatment and WES might be an effective supplementary technique for detection of potential gene mutations and providing accurate treatment for metastatic breast cancer patients.
- Published
- 2021
- Full Text
- View/download PDF
7. THBS1/CD47 Modulates the Interaction of γ-Catenin With E-Cadherin and Participates in Epithelial–Mesenchymal Transformation in Lipid Nephrotoxicity
- Author
-
Li Gao, Ting-ting Yang, Jun-sheng Zhang, Hong-xia Liu, Dong-cheng Cai, Lin-tao Wang, Jing Wang, Xin-wei Li, Kun Gao, Su-ya Zhang, Yu-jia Cao, Xiao-xia Ji, Miao-miao Yang, Biao Han, Sheng Wang, Lu He, Xiao-yan Nie, Dan-mei Liu, Gang Meng, and Chao-yong He
- Subjects
cd47 ,E-cadherin ,γ-catenin ,fibrosis ,inflammation ,lipid nephrotoxicity ,Biology (General) ,QH301-705.5 - Abstract
Hyperlipidemia, an important risk factor for cardiovascular and end-stage renal diseases, often aggravates renal injury and compromises kidney function. Here, histological analysis of human kidney samples revealed that high lipid levels induced the development of renal fibrosis. To elucidate the mechanism underlying lipid nephrotoxicity, we used two types of mouse models (Apoe−/− and C57BL/6 mice fed a 45 and 60% high-fat diet, respectively). Histological analysis of kidney tissues revealed high-lipid-induced renal fibrosis and inflammation; this was confirmed by examining fibrotic and inflammatory marker expression using Western blotting and real-time polymerase chain reaction. Oxidized low-density lipoprotein (OX-LDL) significantly induced the fibrotic response in HK-2 tubular epithelial cells. RNA-sequencing and Gene Ontology analysis of differentially expressed mRNAs in OX-LDL-treated HK-2 tubular epithelial cells and real-time PCR validation in Apoe−/− mice showed that the expression of thrombospondin-1 (THBS1) in the high-fat group was significantly higher than that of the other top known genes, along with significant overexpression of its receptor CD47. THBS1 knockdown cells verified its relation to OX-LDL-induced fibrosis and inflammation. Liquid chromatography tandem mass spectrometry and STRING functional protein association network analyses predicted that THBS1/CD47 modulated the interaction between γ-catenin and E-cadherin and was involved in epithelial–mesenchymal transition, which was supported by immunoprecipitation and immunohistochemistry. CD47 downregulation following transfection with small-hairpin RNA in OX-LDL-treated tubular epithelial cells and treatment with anti-CD47 antibody restored the expression of E-cadherin and attenuated renal injury, fibrosis, and inflammatory response in OX-LDL-treated cells and in type 2 diabetes mellitus. These findings indicate that CD47 may serve as a potential therapeutic target in long-term lipid-induced kidney injury.
- Published
- 2021
- Full Text
- View/download PDF
8. The role of adjuvant chemotherapy in stage I–III male breast cancer: a SEER-based analysis
- Author
-
Wei-Ping Li, Hong-Fei Gao, Fei Ji, Teng Zhu, Min-Yi Cheng, Mei Yang, Ci-Qiu Yang, Liu-Lu Zhang, Jie-Qing Li, Jun-Sheng Zhang, and Kun Wang
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background and aims: Male breast cancer is an uncommon disease. The benefit of adjuvant chemotherapy in the treatment of male breast cancer patients has not been determined. The aim of this study was to explore the value of adjuvant chemotherapy in men with stage I–III breast cancer, and we hypothesized that some male patients may safely skip adjuvant chemotherapy. Methods: Male breast cancer patients between 2010 and 2015 from the Surveillance Epidemiology and End Results database were included. Univariate and multivariate Cox analyses were used to analyse the factors associated with survival. The propensity score matching method was adopted to balance baseline characteristics. Kaplan–Meier curves were used to evaluate the impacts of adjuvant chemotherapy on survival. The primary endpoint was survival. Results: We enrolled 514 patients for this study, including 257 patients treated with chemotherapy and 257 patients without. There was a significant difference in overall survival (OS) but not in breast cancer-specific survival (BCSS) between the two groups ( p 2 cm, lymph node-positive male breast cancer patients ( p 0.05, BCSS: p > 0.05). Adjuvant chemotherapy showed no significant effects on both OS and BCSS in patients with stage I (OS: p = 0.100, BCSS: p = 0.858) and stage IIA breast cancer (OS: p > 0.05, BCSS: p > 0.05). Conclusion: For stage I and stage IIA patients, adjuvant chemotherapy could not improve OS and BCSS. Therefore, adjuvant chemotherapy might be skipped for stage I and stage IIA male breast cancer patients.
- Published
- 2020
- Full Text
- View/download PDF
9. Elucidation of Arctigenin Pharmacokinetics and Tissue Distribution after Intravenous, Oral, Hypodermic and Sublingual Administration in Rats and Beagle Dogs: Integration of In Vitro and In Vivo Findings
- Author
-
Jie Li, Xin Li, Yu-Shan Ren, Yuan-Yuan Lv, Jun-Sheng Zhang, Xiao-Li Xu, Xian-Zhen Wang, Jing-Chun Yao, Gui-Min Zhang, and Zhong Liu
- Subjects
arctigenin ,pharmacokinetics ,UPLC/MS/MS ,bioavailability ,microsomes ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Although arctigenin (AG) has diverse bioactivities, such as anti-oxidant, anti-inflammatory, anti-cancer, immunoregulatory and neuroprotective activities, its pharmacokinetics have not been systematically evaluated. The purpose of this work was to identify the pharmacokinetic properties of AG via various experiments in vivo and in vitro. In this research, rats and beagle dogs were used to investigate the PK (pharmacokinetics, PK) profiles of AG with different drug-delivery manners, including intravenous (i.v), hypodermic injection (i.h), and sublingual (s.l) administration. The data shows that AG exhibited a strong absorption capacity in both rats and beagle dogs (absorption rate < 1 h), a high absorption degree (absolute bioavailability > 100%), and a strong elimination ability (t1/2 < 2 h). The tissue distributions of AG at different time points after i.h showed that the distribution of AG in rat tissues is rapid (2.5 h to reach the peak) and wide (detectable in almost all tissues and organs). The AG concentration in the intestine was the highest, followed by that in the heart, liver, pancreas, and kidney. In vitro, AG were incubated with human, monkey, beagle dog and rat liver microsomes. The concentrations of AG were detected by UPLC-MS/MS at different time points (from 0 min to 90 min). The percentages of AG remaining in four species’ liver microsomes were human (62 ± 6.36%) > beagle dog (25.9 ± 3.24%) > rat (15.7 ± 9%) > monkey (3.69 ± 0.12%). This systematic investigation of pharmacokinetic profiles of arctigenin (AG) in vivo and in vitro is worthy of further exploration.
- Published
- 2017
- Full Text
- View/download PDF
10. Identification of Small-Molecule Bioactive Constituents from the Leaves of
- Author
-
Yin-Yin, Wang, Jun-Sheng, Zhang, Xin-Xin, Wang, Lin-Lin, Tian, Yu-Peng, Li, Chao, Wang, Ren-Fen, Ma, Yi-Ke, Yin, Jie, Bao, and Hua, Zhang
- Abstract
The health benefits of
- Published
- 2022
11. Antiradical Aromatic Constituents from Pleurotus eryngii
- Author
-
Xin-Xin Cao, Hua Zhang, Chao Liu, Jun Sheng Zhang, and Jin-Yue Sun
- Subjects
Pharmacology ,biology ,Chemistry ,Organic Chemistry ,Drug Discovery ,Pleurotus eryngii ,Plant Science ,Food science ,biology.organism_classification - Abstract
Six aromatic compounds including a new (Z)-ferulic acid ester (1) and a new syringic acid ester (2), as well as four known ferulic acid esters (3–6), were isolated from a well-known edible mushroom, Pleurotus eryngii. Structures of these aromatics were unambiguously characterized via spectroscopic methods especially MS and NMR techniques. All the isolates were assessed for their antiradical effect and NO production inhibitory activity in RAW264.7 macrophages, and compounds 1 and 3 displayed mild DPPH radical removing activity, with IC50 values of 25.0 and 21.6 mM, respectively.
- Published
- 2021
- Full Text
- View/download PDF
12. Sequencing and analysis of the complete mitochondrial genome of Ochotona hyperborea from China and its phylogenetic analysis
- Author
-
Jun-Sheng Zhang, Zhong-Wan Piao, Zhu Liu, Qi Zhang, Xin-Xu Zhao, Qian-Qian Yao, and Qiao-Jiang Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetics ,Mitochondrial DNA ,biology ,Phylogenetic tree ,mitogenome ,Long pcr ,Coreana ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Genome ,phylogenetic trees ,03 medical and health sciences ,Ochotona hyperborea ,030104 developmental biology ,Control region ,Transfer RNA ,Molecular Biology ,Gene ,Mitogenome Announcement ,Research Article - Abstract
The complete mitogenome sequence of Ochotona hyperborea was determined using long PCR. The genome was 17,063 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall base composition of the heavy strand is A (31.1%), C (28.7%), T (26.3%), and G (13.9%). The base compositions present clearly the A–T skew, which is most obvious in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of Ochotona hyperborea in Ochotonidae at the molecular level. The mitochondrial genome would be a significant supplement for the Ochotona hyperborea genetic background. The eight Ochotona species formed a monophyletic group with the high bootstrap value (100%) in all examinations.
- Published
- 2021
13. Orientation-dependent Fiber-optic Inclinometer Based on Core-offset Michelson Interferometer
- Author
-
Hua-jie WANG, Lai-fang ZHENG, and Jun-sheng ZHANG
- Abstract
An in-fiber Michelson interferometer (MI) inclinometer, which consists of misalignment-spliced fiber with end coating, is proposed and experimentally demonstrated. The incident light divided at the misalignment-spliced joint is reflected at the end coating, and then re-coupled into the core of the fiber. Due to the phase difference between the core mode and the cladding mode, a typical Michelson interference is obtained. The fiber near the misalignment-spliced joint is inserted in two capillary quartz tubes. The inclination of the capillary quartz tube leads to a significant deformation and curvature of the misalignment-spliced joint, which causes the wavelength and amplitude of the MI spectrum to change. The experimental results indicate a good response within the angle range of 0º to 50º. Both the wavelength modulation and intensity modulation are realized, with sensitivities of 0.55 nm/deg and 0.17 dB/deg, respectively. Moreover, the sensor shows a strong orientation dependence due to the asymmetric structure in the misalignment-spliced joint.
- Published
- 2021
- Full Text
- View/download PDF
14. Peritoneal Metastasis After Treated With Abemaciclib Plus Fulvestrant for Metastatic Invasive Lobular Breast Cancer: A Case Report and Review of the Literature
- Author
-
Qiangzu Zhang, Min-Yi Cheng, Hong-Fei Gao, Fei Ji, Teng Zhu, Liulu Zhang, Ci-Qiu Yang, Kun Wang, Mei Yang, Gang Niu, Jieqing Li, and Jun-Sheng Zhang
- Subjects
Oncology ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Lobular carcinoma ,lobular carcinoma ,Case Report ,Gene mutation ,Diseases of the endocrine glands. Clinical endocrinology ,whole exome sequencing ,neoplasm metastasis ,Breast cancer ,Endocrinology ,Internal medicine ,Ascites ,medicine ,skin and connective tissue diseases ,breast ,Fulvestrant ,business.industry ,Bone metastasis ,RC648-665 ,medicine.disease ,peritoneum ,Metastatic breast cancer ,body regions ,Invasive lobular carcinoma ,medicine.symptom ,business ,medicine.drug - Abstract
Peritoneal metastases from invasive lobular carcinoma (ILC) of breast are uncommon and usually related to poor prognosis due to difficulty of detection in clinical practice and drug resistance. Therefore, recognizing the entities of peritoneal metastases of ILC and the potential mechanism of drug resistance is of great significance for early detection and providing accurate management. We herein report a case of a 60-year-old female who presented with nausea and vomiting as the first manifestation after treated with abemaciclib (a CDK4/6 inhibitor) plus fulvestrant for 23 months due to bone metastasis of ILC. Exploratory laparotomy found multiple nodules in the peritoneum and omentum, and immunohistochemistry confirmed that the peritoneal metastatic lesions were consistent with ILC. Palliative therapy was initiated, but the patient died two months later due to disease progression with malignant ascites. Whole exome sequencing (WES) was used to detect the tumor samples and showed the peritoneal metastatic lesions had acquired ESR1 and PI3KCA mutations, potentially explaining the mechanism of endocrine therapy resistance. We argue that early diagnosis of peritoneal metastasis from breast cancer is crucial for prompt and adequate treatment and WES might be an effective supplementary technique for detection of potential gene mutations and providing accurate treatment for metastatic breast cancer patients.
- Published
- 2021
15. Study on Magnets for Beam Transport System of SC200 Superconducting Proton Therapy Facility
- Author
-
Zheng Jinxing, Jun-Sheng Zhang, Yuntao Song, and Liu Haiyang
- Subjects
Physics ,business.industry ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,Magnetic field ,Dipole ,Optics ,Electromagnetic coil ,Magnet ,Quadrupole ,Physics::Accelerator Physics ,Electrical and Electronic Engineering ,Quadrupole magnet ,business ,Proton therapy ,Beam (structure) - Abstract
The SC200 proton therapy facility is consisting of a superconducting cyclotron, a gantry treatment room and a fixed beam room. The proton beams are transported mainly with beam transport devices, such as dipole magnets, quadrupole magnets, and corrector magnets which lie on the beam transport line layout of which is based on the beam optics design. A series of dipole, quadrupole, and corrector magnets ultimately make high-quality transmission of proton beams possible. Dipole magnets are mainly for beams deflection, quadrupole magnets are for beam focusing, and corrector magnets for orbit correction of beams. This paper shows the design of such magnets for the beam transport line. Besides, main parameters of magnets are presented and magnetic analysis work is also illustrated in this paper. The magnetic fields of dipole and quadrupole magnets are measured by hall mapping system and rotary coil measuring system. The results show that the measuring results are a little worse than the simulation results. However, both methods can meet the design requirement and the basic trend is similar to each other.
- Published
- 2019
- Full Text
- View/download PDF
16. A new tigliane-type diterpenoid from Euphorbia tirucalli
- Author
-
Han-Zhuang Weng, Gui-Hua Tang, Sheng Yin, Jun-Sheng Zhang, Yang Tian, and Jia-Luo Huang
- Subjects
P-glycoprotein Inhibitor ,biology ,010405 organic chemistry ,Euphorbia tirucalli ,Chemistry ,Stereochemistry ,Organic Chemistry ,Plant Science ,Ring (chemistry) ,biology.organism_classification ,01 natural sciences ,Biochemistry ,Terpenoid ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry - Abstract
Five tigliane (1−5) and one ingenane (6) diterpenoids were isolated from the ethanol extract of Euphorbia tirucalli. The structures of these compounds were identified based on analysis of their spectroscopic data. Among them, compound 12-O-(2E,4E,6E,8E-tetradecatetraenoyl)-13-O-isobutyroyl-4β-deoxyphorbol (1) was a new tigliane. The Rho123 effluxion assay showed that tiglianes with a trans-fused 5/7 ring system such as compounds 1, 2, and 4 had potent inhibitory activity against P-glycoprotein in HepG2/ADR cells.
- Published
- 2021
- Full Text
- View/download PDF
17. Sequencing and analysis of the complete mitochondrial genome of the lesser bandicoot rat (Bandicota bengalensis) from China and its phylogenetic analysis
- Author
-
Zhu, Liu, primary, Qing-Qing, Wang, additional, Xue-Ting, Jiang, additional, Wen-Jing, Jiang, additional, Xin-Xu, Zhao, additional, Qian-Qian, Yao, additional, Jun-Sheng, Zhang, additional, and Zhong-Wan, Piao, additional
- Published
- 2021
- Full Text
- View/download PDF
18. Comparison of Overall Survival Between Invasive Lobular Breast Carcinoma and Invasive Ductal Breast Carcinoma: A Propensity Score Matching Study Based on SEER Database
- Author
-
Weijun Pan, Jun-Sheng Zhang, Yi Zhang, Mei Yang, Kun Wang, Chuqian Lei, Fei Ji, Jieqing Li, Ci-Qiu Yang, Hong-Fei Gao, and Liulu Zhang
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,overall survival ,Subgroup analysis ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,breast cancer ,Internal medicine ,invasive lobular carcinoma ,medicine ,invasive ductal carcinoma ,SEER program ,Stage (cooking) ,skin and connective tissue diseases ,neoplasms ,Survival analysis ,Original Research ,business.industry ,Cancer ,Retrospective cohort study ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,body regions ,030104 developmental biology ,030220 oncology & carcinogenesis ,Invasive lobular carcinoma ,business ,Invasive Lobular Breast Carcinoma - Abstract
ObjectiveInvasive lobular carcinoma (ILC) and invasive ductal carcinoma (IDC) account for most breast cancers. However, the overall survival (OS) differences between ILC and IDC remain controversial. This study aimed to compare nonmetastatic ILC to IDC in terms of survival and prognostic factors for ILC.MethodsThis retrospective cohort study used data from the Surveillance, Epidemiology and End Results (SEER) Cancer Database (www.seer.cancer.gov). Women diagnosed with nonmetastatic ILC and IDC between 2006 and 2016 were included. A propensity score matching (PSM) method was used in our analysis to reduce baseline differences in clinicopathological characteristics and survival outcomes. Kaplan-Meier curves and log-rank test were used for survival analysis.ResultsCompared to IDC patients, ILC patients were diagnosed later in life with poorly differentiated and larger lesions, as well as increased expression of estrogen receptors (ERs) and/or progesterone receptors (PRs). A lower rate of radiation therapy and chemotherapy was observed in ILC. After PSM, ILC, and IDC patients exhibited similar OS (HR=1.017, p=0.409, 95% CI: 0.967–1.069). In subgroup analysis of HR-negative, AJCC stage III, N2/N3 stage patients, or those who received radiotherapy, ILC patients exhibited worse OS compared to IDC patients. Furthermore, multivariate analysis revealed a 47% survival benefit for IDC compared to ILC in HR-negative patients who received chemotherapy (HR=1.47, p=0.01, 95% CI: 1.09–1.97).ConclusionsOur results demonstrated that ILC and IDC patients had similar OS after PSM. However, ILC patients with high risk indicators had worse OS compared to IDC patients by subgroup analysis.
- Published
- 2020
- Full Text
- View/download PDF
19. Cytotoxic macrocyclic diterpenoids from Jatropha multifida
- Author
-
Sheng Yin, Shen Li, Yao Zhang, Gui-Hua Tang, Abrar Ahmed, and Jun-Sheng Zhang
- Subjects
Models, Molecular ,Macrocyclic Compounds ,Stereochemistry ,Apoptosis ,Jatropha ,Crystallography, X-Ray ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Cell Line, Tumor ,Neoplasms ,Drug Discovery ,Ic50 values ,Humans ,Cytotoxic T cell ,Cytotoxicity ,Molecular Biology ,010405 organic chemistry ,Chemistry ,Cell Cycle ,Organic Chemistry ,Absolute configuration ,Cell cycle ,Antineoplastic Agents, Phytogenic ,0104 chemical sciences ,Cell culture ,Diterpenes ,Jatropha multifida - Abstract
Nine new macrocyclic diterpenoids (1–9), jatromultones A-I, along with eight known analogues (10–17) were isolated from the trunks of Jatropha multifida. The structures of the new compounds, including their absolute configurations, were elucidated by combination of spectroscopic analysis, single crystal X-ray diffraction, Rh2(OCOCF3)4-induced CD method, and chemical correlations. All compounds were screened for the cytotoxicity against five cancer cell lines, including one drug-resistant cell line, and seven compounds exhibited significant activity with IC50 values less than 10 μM. Compound 4 with IC50 values ranging from 2.69 to 6.44 μM toward all cell lines was selected for further mechanistic study, which showed that 4 could arrest cell cycle at G2/M phase and induce apoptosis. The brief structure-activity relationships (SARs) of these macrocyclic diterpenoids were also discussed.
- Published
- 2018
- Full Text
- View/download PDF
20. Diterpenoid glucosides with cystathionine γ-lyase inhibitory activity from Tinospora sinensis
- Author
-
Kongkai Zhu, Youtian Hu, De-Feng Xu, Jun-Sheng Zhang, Kun-Sheng Song, Fang Wu, and Hua Zhang
- Subjects
Tinospora ,Circular dichroism ,Stereochemistry ,Cystathionine γ lyase ,Inhibitory postsynaptic potential ,Biochemistry ,Structure-Activity Relationship ,Glucosides ,Tinospora sinensis ,parasitic diseases ,Drug Discovery ,Humans ,Enzyme Inhibitors ,Molecular Biology ,Density Functional Theory ,chemistry.chemical_classification ,Dose-Response Relationship, Drug ,Molecular Structure ,biology ,Organic Chemistry ,Cystathionine gamma-Lyase ,Absolute configuration ,Cystathionine beta synthase ,Terpenoid ,Enzyme ,chemistry ,biology.protein ,Diterpenes - Abstract
Fifteen previously undescribed nor-clerodane diterpenoid glucosides tinosinesides C-Q (1-15), along with four known analogues (16-19), were isolated from the stems of Tinospora sinensis. The structures of the new compounds were elucidated by spectroscopic means, and their absolute configurations were established on the basis of time-dependent density functional theory (TD-DFT) based electronic circular dichroism (ECD) calculation and chemical methods. All the isolates were evaluated for their inhibitory effects on cystathionine γ-lyase (CSE), a natural enzyme responsible for the synthesis of H2S. Compounds 4 and 5 represent rare examples of natural CSE inhibitors and the possible binding mode to CSE was further probed by molecular docking experiment.
- Published
- 2021
- Full Text
- View/download PDF
21. Sequencing and analysis of the complete mitochondrial genome of Ochotona coreana from China and its phylogenetic analysis
- Author
-
Jun-Sheng, Zhang, primary, Bo-Qi, Li, additional, Qiao-Jiang, Yang, additional, Qi, Zhang, additional, Chen, Wang, additional, and Zhu, Liu, additional
- Published
- 2020
- Full Text
- View/download PDF
22. Lindera cyclopentenedione intermediates from the roots of Lindera aggregata
- Author
-
Sheng Yin, Wei Li, Lin Chen, Jun-Jie Deng, Jun-Sheng Zhang, Gui-Hua Tang, Bo Liu, and Abrar Ahmed
- Subjects
Lindera ,biology ,010405 organic chemistry ,Chemistry ,Stereochemistry ,General Chemical Engineering ,General Chemistry ,010402 general chemistry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Lindera aggregata ,chemistry.chemical_compound ,Monomer ,Biosynthesis ,Enantiomer - Abstract
Chromatographic fractionation of the roots of Lindera aggregata has led to the isolation of three new monomers of Lindera cyclopentenedione derivatives (1–3), a pair of new enantiomers of bi-linderone derivatives (4a/4b), and six known Lindera cyclopentenediones (5–8 and 9a/9b). Their structures were determined by NMR and MS data. The absolute configurations of the new bi-linderone derivative enantiomers (4a/4b) were determined by ECD calculation. (±)-Lindepentone A (1) presents the novel skeleton of 3,5-dioxocyclopent-1-enecarboxylate. Lindoxepines A (2) and B (3) present an unprecedented oxepine-2,5-dione derivative skeleton, which may be enlightening for the in vivo biosynthesis of the monomers of Lindera cyclopentenediones. A possible biosynthetic pathway for 1 and a plausible biosynthetic pathway from stilbenes to Lindera cyclopentenediones via the key intermediates 2 and 3 were postulated. The inhibitory activity of these compounds against three microorganisms was also evaluated.
- Published
- 2018
- Full Text
- View/download PDF
23. Thiophene enantiomers from the aerial parts of Eclipta prostrata
- Author
-
Jie Bao, Jin-Hai Yu, Hei He, Jun-Sheng Zhang, Shu-Juan Yu, and Hua Zhang
- Subjects
Pharmacology ,biology ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Pharmaceutical Science ,General Medicine ,biology.organism_classification ,01 natural sciences ,Thiophene derivatives ,0104 chemical sciences ,Analytical Chemistry ,Chiral column chromatography ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,Drug Discovery ,Thiophene ,Molecular Medicine ,Organic chemistry ,Enantiomer ,Antibacterial activity ,Derivatization ,Eclipta prostrata - Abstract
Ten thiophene derivatives (1-10), including two previously undescribed ones (1 and 2), have been obtained and structurally characterized from the aerial parts of a traditional Chinese herb Eclipta prostrata. Six of them with one chiral center were identified to be scalemic mixtures, and the pure enantiomers of two isolates (1 and 3) were successfully separated via chemical derivatization and chiral HPLC, with the absolute configurations being established by analysis of optical rotations. All the thiophenes were subjected to a series of assays and compounds 9 and 10 exhibited mild antibacterial activity against Staphylococcus aureus.[Formula: see text].
- Published
- 2020
- Full Text
- View/download PDF
24. Bioactive sesquiterpenoids from the flower buds of Tussilago farfara
- Author
-
Xiu-Qing Song, Jun-Sheng Zhang, Jia Sun, Hua Zhang, Jin-Hai Yu, and Kai-Lu Liu
- Subjects
Circular dichroism ,Magnetic Resonance Spectroscopy ,Stereochemistry ,Molecular Conformation ,Apoptosis ,Tussilago ,Flowers ,01 natural sciences ,Biochemistry ,chemistry.chemical_compound ,Cell Line, Tumor ,Drug Discovery ,Humans ,Glycoside Hydrolase Inhibitors ,Benzofuran ,Molecular Biology ,Cell Proliferation ,biology ,010405 organic chemistry ,Chemistry ,Circular Dichroism ,Organic Chemistry ,Absolute configuration ,Stereoisomerism ,alpha-Glucosidases ,Carbon-13 NMR ,biology.organism_classification ,0104 chemical sciences ,Benzopyran ,010404 medicinal & biomolecular chemistry ,S Phase Cell Cycle Checkpoints ,Enantiomer ,Sesquiterpenes ,Derivative (chemistry) - Abstract
Eleven new compounds including five bisabolane (1-5) and three oplopane (6-8) sesquiterpenoids, a pair of benzopyran enantiomers (9 & 10) and a benzofuran derivative (11), along with six known sesquiterpenoid co-metabolites (12-17), have been obtained from the flower buds of Tussilago farfara. Their structures were elucidated by comprehensive spectroscopic analyses and comparison with structurally related known analogues. The absolute configurations of all the compounds except 11 were unequivocally assigned by various techniques, including Mosher's method and time-dependent density functional theory (TD-DFT) based calculations of 13C NMR and electronic circular dichroism (ECD) data. The C-8 absolute configuration on the sidechain of this group of bisabolane sesquiterpenoids was assigned for the first time. Our bioassays have established that compounds 3, 4, 13 and 14 showed significant α-glucosidase inhibitory activities, while 6, 8 and 14 displayed moderate antiproliferative effects against two human tumor cell lines A549 and MDA-MB-231. Further flow cytometric analysis revealed that 14 effectively induced cell apoptosis and arrested cell cycle at the S/G2 phases in A549 cells, in a dose-dependent manner.
- Published
- 2021
- Full Text
- View/download PDF
25. New prenylated coumarins from the stems of Toddalia asiatica
- Author
-
Jia-Luo Huang, Min‐Hong Jiang, You-Kai Xu, Wei Li, Jun-Sheng Zhang, Sheng Yin, Gui-Hua Tang, and Abrar Ahmed
- Subjects
010405 organic chemistry ,Stereochemistry ,General Chemical Engineering ,General Chemistry ,Phenolic acid ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Prenylation ,Toddalia asiatica ,Ic50 values ,Enantiomer ,Derivative (chemistry) - Abstract
Eight new prenylated coumarins (1a/1b, 2a/2b, and 3–6) including two pairs of enantiomers (1a/1b and 2a/2b), a new phenolic acid derivative, methyl (E)-3,4-bis(4-hydroxyphenyl)-4-oxobut-2-enoate (7), and 33 known compounds (8–40) were isolated from the stems of Toddalia asiatica. Their structures were established from spectroscopic data and by chemical methods. The absolute configurations of two pairs of enantiomers (1a/1b and 2a/2b) were determined by X-ray diffraction analysis together with ECD and specific optical rotation calculations. The inhibitory effects of selected compounds against phosphodiesterase-4 (PDE4) were evaluated, and compounds 12, 19, 21–23, 26, 34, and 35 exhibited PDE4 inhibition activities with IC50 values less than 10 μM.
- Published
- 2017
- Full Text
- View/download PDF
26. The complete mitochondrial genome of the striped hamster (Cricetulus barabesis) China and its phylogenetic analysis
- Author
-
Zhu, Liu, primary, Ke-Song, Qin, additional, Bo-Qi, Li, additional, Cheng-Wen, Yu, additional, He, Cai, additional, Jun-Sheng, Zhang, additional, Jin-Xun, Li, additional, and Zhu, Wang, additional
- Published
- 2019
- Full Text
- View/download PDF
27. Sequencing and analysis of the complete mitochondrial genome of Crocidura tanakae from China and its phylogenetic analysis
- Author
-
Zhi-Min, Jin, primary, Ke-Song, Qin, additional, Bo-Qi, Li, additional, Jun-Sheng, Zhang, additional, He, Cai, additional, Jin-Xun, Li, additional, Zhu, Wang, additional, and Zhu, Liu, additional
- Published
- 2019
- Full Text
- View/download PDF
28. Polycyclic polyprenylated acylphloroglucinols: natural phosphodiesterase-4 inhibitors from Hypericum sampsonii
- Author
-
Sheng Yin, Yi-Hong Zou, Zhen-Zhen Li, Gui-Hua Tang, Jun-Sheng Zhang, and Yan-Qiong Guo
- Subjects
Bicyclic molecule ,010405 organic chemistry ,Stereochemistry ,General Chemical Engineering ,Substrate (chemistry) ,General Chemistry ,010402 general chemistry ,Ring (chemistry) ,01 natural sciences ,Adenosine ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,medicine ,Nonane ,Undecane ,IC50 ,Rolipram ,medicine.drug - Abstract
Chemical investigation of the aerial parts of Hypericum sampsonii led to the isolation of seven new polycyclic polyprenylated acylphloroglucinols, hypersampsonones A–G (1–7), together with 23 known analogs (8–30). Their structures including the absolute configurations were elucidated by combined spectroscopic analysis, quantum chemical ECD calculations, and chemical methods. Compound 1 represents an unprecedented cyclocitral monoterpene-coupled bicyclo[3.3.1]nonane skeleton, while 2 features an unusual hexahydrofuro[2,3-b]furan-diepoxy ring system fused in a tricyclo[4.3.1.15,7]undecane skeleton. All the compounds were screened by using tritium-labeled adenosine 3′,5′-cyclic monophosphate ([3H]-cAMP) as substrate for their inhibitory activity against phosphodiesterase-4 (PDE4), which is a drug target for the treatment of asthma and chronic obstructive pulmonary disease. Compounds 1, 18–19, 21, and 25–30 exhibited inhibition with IC50 values less than 10 μM, in which compound 19 represented the most active compound (IC50 = 0.64 μM), being comparable to the positive control, rolipram (IC50 = 0.62 μM).
- Published
- 2016
- Full Text
- View/download PDF
29. Biscembranoids and Cembranoids from the Soft Coral Sarcophyton elegans
- Author
-
Gui-Hua Tang, Yi-Hong Zou, Yun-Shao Xu, Abrar Ahmed, Jun-Sheng Zhang, Man-Xi Ge, Lan-Lan Lou, Yunyun Chen, Sheng Yin, and Wei Li
- Subjects
South china ,Stereochemistry ,Coral ,Pharmaceutical Science ,Biology ,Crystallography, X-Ray ,Nitric Oxide ,01 natural sciences ,Article ,cembranoids ,Cell Line ,Mice ,X-Ray Diffraction ,Drug Discovery ,Ic50 values ,Animals ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Molecular Structure ,Sarcophyton elegans ,010405 organic chemistry ,biscembranoids ,NO inhibition ,Anthozoa ,Cycloaddition ,0104 chemical sciences ,Biosynthetic Pathways ,010404 medicinal & biomolecular chemistry ,Diterpenes - Abstract
Two novel biscembranoids, sarelengans A and B (1 and 2), five new cembranoids, sarelengans C-G (3-7), along with two known cembranoids (8 and 9) were isolated from the South China Sea soft coral Sarcophyton elegans. Their structures were determined by spectroscopic and chemical methods, and those of 1, 4, 5, and 6 were confirmed by single crystal X-ray diffraction. Compounds 1 and 2 represent the first example of biscembranoids featuring a trans-fused A/B-ring conjunction between the two cembranoid units. Their unique structures may shed light on an unusual biosynthetic pathway involving a cembranoid-∆⁸ rather than the normal cembranoid-∆¹ unit in the endo-Diels-Alder cycloaddition. Compounds 2 and 3 exhibited potential inhibitory effects on nitric oxide production in RAW 264.7 macrophages, with IC50 values being at 18.2 and 32.5 μM, respectively.
- Published
- 2017
30. Fringe field interference effects on field quality for quadrupole magnets
- Author
-
Jun-Sheng Zhang, Wu-Quan Zhang, and Jian Zhou
- Subjects
Superconductivity ,Physics ,Nuclear and High Energy Physics ,010308 nuclear & particles physics ,business.industry ,Field homogeneity ,Cyclotron ,01 natural sciences ,law.invention ,Optics ,Beamline ,law ,0103 physical sciences ,Homogeneity (physics) ,Field quality ,010306 general physics ,business ,Quadrupole magnet ,Multipole expansion ,Instrumentation - Abstract
A study of fringe field interference effects on field quality of quadrupole magnets for a newly developed superconducting proton cyclotron beamline was presented in this paper. The integral field gradient homogeneity was better than ± 5 × 10E-4 without fringe field interference. The influence of fringe field interference on field quality and integral field degradation was analyzed with 3D simulations. The results showed that the field quality was not affected when only yoke interference was considered. Besides, the characteristics of field quality with current interference were also studied. The integral field degradation and multipole field components were analyzed to research the variation of field quality. It showed that the inhomogeneity of integral field degradation at different beam orbits was the main disturbance for integral field quality. Finally, a normalized formula of the integral field degradation with different parameters was established, which will be the data reference for the newly developed superconducting proton cyclotron beam line assembly in the near future.
- Published
- 2019
- Full Text
- View/download PDF
31. Natural nitric oxide (NO) inhibitors from Aristolochia mollissima
- Author
-
Bao Cheng, Qiong Gu, Sheng Yin, Jun-Sheng Zhang, Jing-Mei Bao, Zhongbin Cheng, Zhang-Hua Sun, Gui-Hua Tang, and Zhen Dong
- Subjects
Quantum chemical ,chemistry.chemical_compound ,chemistry ,Lipopolysaccharide ,Stereochemistry ,General Chemical Engineering ,Ic50 values ,Positive control ,General Chemistry ,Quercetin ,IC50 ,Aristolochia mollissima ,Nitric oxide - Abstract
Six new sesquiterpenoids, aristomollins A–F (1–6), and 24 known analogues (7–30) were isolated from the leaves and stems of Aristolochia mollissima. Their structures were elucidated by spectroscopic analysis, and the absolute configurations of compounds 2–5 were determined by the chemical correlations and quantum chemical ECD calculations. Compound 1 represented an unprecedented 5,6-seco-4,5-cyclohumulane skeleton. All the compounds were examined for their inhibitory effects on the nitric oxide (NO) production induced by lipopolysaccharide (LPS) in BV-2 microglial cells, and compounds 4, 9, 28, and 30 exhibited pronounced inhibition of NO production with IC50 values in the range of 5.7–9.9 μM, being more active than the positive control, quercetin (IC50 = 15.7 μM).
- Published
- 2014
- Full Text
- View/download PDF
32. A new bisabolane sesquiterpenoid and a new abietane diterpenoid from Cephalotaxus sinensis
- Author
-
Yi-Hong Zou, Wei Li, Pek-Ha Sam, Abrar Ahmed, Sheng Yin, Jun-Sheng Zhang, and Gui-Hua Tang
- Subjects
Magnetic Resonance Spectroscopy ,Stereochemistry ,Phytochemicals ,Plant Science ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Cephalotaxus ,chemistry.chemical_compound ,Abietane ,Cephalotaxus sinensis ,biology ,Molecular Structure ,010405 organic chemistry ,Spectrum Analysis ,Organic Chemistry ,Nuclear magnetic resonance spectroscopy ,biology.organism_classification ,Terpenoid ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry ,Phytochemical ,Abietanes ,Spectrum analysis ,Sesquiterpenes - Abstract
Phytochemical investigation of Cephalotaxus sinensis has led to the isolation of a new bisabolane sesquiterpenoid (1), a new abietane diterpenoid (2), and 13 known compounds (3–15). Their structures were elucidated by extensive spectroscopic analysis (MS, UV, IR, and NMR).
- Published
- 2017
- Full Text
- View/download PDF
33. A new selaginellin derivative and a new triarylbenzophenone analog from the whole plant of Selaginella pulvinata
- Author
-
You-Kai Xu, Sheng Yin, Xin Liu, Han-Zhuang Weng, Gui-Hua Tang, and Jun-Sheng Zhang
- Subjects
Selaginellaceae ,Pharmacology ,Molecular Structure ,Cyclohexanones ,010405 organic chemistry ,Stereochemistry ,Chemistry ,Biphenyl Compounds ,Organic Chemistry ,Pharmaceutical Science ,General Medicine ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,Phosphodiesterase-4 ,Complementary and alternative medicine ,Drug Discovery ,Ic50 values ,Molecular Medicine ,Phosphodiesterase 4 Inhibitors ,Selaginella pulvinata ,Selaginellin T - Abstract
Five selaginellin derivatives (1 and 3–6) including a new one, selaginellin T (1), and a new triarylbenzophenone analog, selagibenzophenone A (2), were isolated from the whole plants of Selaginella pulvinata. Their structures were determined by 1D- and 2D-NMR and HR-ESI-MS data. Selagibenzophenone A (2) is the first example of naturally occurring triarylbenzophenone. The results of the phosphodiesterase-4 (PDE4) inhibitory screening assays showed that compounds 1−6 exhibited potent activities with the IC50 values in the range of 1.04−9.35 μM.
- Published
- 2017
- Full Text
- View/download PDF
34. Sequencing and analysis of the complete mitochondrial genome of the Ussuri shrew (Sorex mirabilis) from China
- Author
-
Zhu, Liu, primary, Xin-Min, Tian, additional, Zhi-Min, Jin, additional, Ming, Dong, additional, Jun-Sheng, Zhang, additional, and Jian-Yu, Du, additional
- Published
- 2017
- Full Text
- View/download PDF
35. Sequencing and analysis of the complete mitochondrial genome of the slender shrew (Sorex gracillimus) from China
- Author
-
Zhu, Liu, primary, Xin-Min, Tian, additional, Jian-Li, Jin, additional, Zhi-Min, Jin, additional, Dian-Wei, Li, additional, and Jun-Sheng, Zhang, additional
- Published
- 2017
- Full Text
- View/download PDF
36. Bioactive Cembranoids from the South China Sea Soft Coral Sarcophyton elegans
- Author
-
Cheng-Qi Fan, Qiao Liu, Gui-Hua Tang, Xin Liu, Hongsheng Wang, Jun-Sheng Zhang, and Sheng Yin
- Subjects
South china ,Coral ,Pharmaceutical Science ,Zoology ,Biology ,Article ,cembranoids ,Analytical Chemistry ,lcsh:QD241-441 ,lcsh:Organic chemistry ,Cell Movement ,Cell Line, Tumor ,Drug Discovery ,Animals ,Humans ,Sarcophyton elegans ,Physical and Theoretical Chemistry ,Ecology ,Organic Chemistry ,antimigratory activity ,Anthozoa ,Chemistry (miscellaneous) ,Molecular Medicine ,Female ,Diterpenes ,Human breast - Abstract
Four new cembranoids, sarcophelegans A–D (1–4) and six known analogues (5–10) were isolated from the South China Sea soft coral Sarcophyton elegans. Their structures were elucidated through detailed spectroscopic analysis, and the absolute configuration of 1 was confirmed by single-crystal X-ray diffraction. The antimigratory potential of compounds 1–10 were evaluated and compounds 2 and 6 were found to inhibit human breast tumor MDA-MB-231 cell migration at 10 μM.
- Published
- 2015
- Full Text
- View/download PDF
37. Sequencing and analysis of the complete mitochondrial genome of flat-skulled shrew (Sorex roboratus) from China
- Author
-
Zhu, Liu, primary, Ao-Nan, Wang, additional, Jun-Sheng, Zhang, additional, Xi, Yang, additional, Huan, Liu, additional, and Zhi-Min, Jin, additional
- Published
- 2017
- Full Text
- View/download PDF
38. Comparison of methimazole/hydrocortisone ointment with oral methimazole in patients with graves disease: A prospective, randomized, open-label, parallel-group, 18-month study
- Author
-
Wen-bo Qi, Jian Zhang, Ling Chen, Hong-qing Wang, Jie Bai, Jun-sheng Zhang, Hui-qing Li, Men Wang, Jun Liang, Yan-yan Gao, and Juan-qing Ren
- Subjects
medicine.medical_specialty ,endocrine system ,endocrine system diseases ,medicine.drug_class ,medicine.medical_treatment ,Graves' disease ,ointment ,Neutropenia ,Gastroenterology ,Article ,Internal medicine ,medicine ,hyperthyroidism ,Pharmacology (medical) ,Adverse effect ,Pharmacology ,treatment ,transdermal administration ,business.industry ,Antithyroid agent ,medicine.disease ,eye diseases ,Surgery ,Tolerability ,antithyroid drugs ,Corticosteroid ,Liver function ,Thyroid function ,business - Abstract
Background: Thionamide antithyroid drugs (ATDs) have certain disadvantages and are associated with some adverse events (AEs). To overcome the problems associated with ATDs, a compound antithyroid ointment (CATO) containing methimazole (MMI) and hydrocortisone has been developed for use as a local thyroid treatment (LTT). Objective: The aim of this study was to assess the clinical effectiveness and tolerability of CATO LTT in patients with Graves disease (GD). Methods: This was a prospective, randomized, open-label, parallel-group clinical trial conducted at the Provincial Hospital Affiliated to Shandong University (Jinan, China). Patients with GD aged 19 to 65 years were randomized to receive either CATO LTT 0.3 g/d or oral MMI 37.5 mg/d (control group) treatment for 18 months, with a 4-year follow-up period. Hyperthyroid symptoms, thyroid function, granulocyte count, liver function, and AEs were assessed at baseline and every 2 weeks until serum thyroid hormone (TH) concentration normalized, at which point patients were assessed monthly. The primary efficacy end points were the duration of treatment required for serum TH concentration to normalize and the remission rate after completing the 18-month treatment regimen. Results: A total of 154 patients (133 women, 21 men; mean [SD] age, 39.6 [11.8] years; all Han Chinese) participated in the study; all patients completed the 18-month treatment period. Compared with the MMI group (n 76), the CATO- treated group (n 78) had a significantly shorter median (range) time to restoration of normal serum thyroid hormone concentration (43 [12–150] vs 22 [7–60] days; P < 0.001), a significantly lower rate of recurrence of hyperthyroidism (309/1520 [20.3%] vs 193/1368 [14.1%] person-time; P < 0.001), a significantly lower drug hypothyroidism rate (185/1520 [12.2%] vs 54/1368 [3.9%] person-time; P < 0.001), and a higher remission rate (year 1:46/69 [66.7%] vs 65/72 [90.3%] patients, P 0.001; year 2:40/69 [58.0%] vs 60/72 [83.3%] patients, P - 0.001; year 3:34/69 [49.3%] vs 57/72 [79.2%] patients, P < 0.001; and year 4:30/69 [43.5%] vs 55/72 [76.4%] patients, P < 0.001). Systemic AEs occurred in 6 patients (7.9%) in the MMI group (drug neutropenia, 2 patients [2.6%]; epistaxis, 1 [1.3%]; hepatopathy, 1 [1.3%]; and other systemic AEs, 2 [2.6%]), while no systemic AEs were observed/reported in the CATO group. Conclusion: This study suggests that CATO LTT was well tolerated and more effective than oral MMI treatment in controlling thyrotoxicosis and promoting remission of GD in these Han Chinese patients.
- Published
- 2008
39. Biscembranoids and Cembranoids from the Soft Coral Sarcophyton elegans.
- Author
-
Wei Li, Yi-Hong Zou, Man-Xi Ge, Lan-Lan Lou, Yun-Shao Xu, Ahmed, Abrar, Yun-Yun Chen, Jun-Sheng Zhang, Gui-Hua Tang, and Sheng Yin
- Abstract
Two novel biscembranoids, sarelengans A and B (1 and 2), five new cembranoids, sarelengans C–G (3–7), along with two known cembranoids (8 and 9) were isolated from the South China Sea soft coral Sarcophyton elegans. Their structures were determined by spectroscopic and chemical methods, and those of 1, 4, 5, and 6 were confirmed by single crystal X-ray diffraction. Compounds 1 and 2 represent the first example of biscembranoids featuring a trans-fused A/B-ring conjunction between the two cembranoid units. Their unique structures may shed light on an unusual biosynthetic pathway involving a cembranoid-Δ
8 rather than the normal cembranoid-Δ¹ unit in the endo-Diels-Alder cycloaddition. Compounds 2 and 3 exhibited potential inhibitory effects on nitric oxide production in RAW264.7 macrophages, with IC50 values being at 18.2 and 32.5 μM, respectively. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.